CN110663387A - Plant13C isotope labeling device - Google Patents
Plant13C isotope labeling device Download PDFInfo
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- CN110663387A CN110663387A CN201911094415.9A CN201911094415A CN110663387A CN 110663387 A CN110663387 A CN 110663387A CN 201911094415 A CN201911094415 A CN 201911094415A CN 110663387 A CN110663387 A CN 110663387A
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- 238000001948 isotopic labelling Methods 0.000 title claims abstract description 23
- 239000004698 Polyethylene Substances 0.000 claims abstract description 67
- -1 polyethylene Polymers 0.000 claims abstract description 67
- 229920000573 polyethylene Polymers 0.000 claims abstract description 67
- 230000001105 regulatory effect Effects 0.000 claims abstract description 12
- 238000010521 absorption reaction Methods 0.000 claims abstract description 9
- 239000007789 gas Substances 0.000 claims description 73
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 238000005406 washing Methods 0.000 claims description 18
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 15
- 229910052802 copper Inorganic materials 0.000 claims description 15
- 239000010949 copper Substances 0.000 claims description 15
- 238000002372 labelling Methods 0.000 claims description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000011229 interlayer Substances 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 2
- 239000010410 layer Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000004868 gas analysis Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 27
- 238000000034 method Methods 0.000 description 11
- 239000000463 material Substances 0.000 description 7
- 238000005485 electric heating Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 239000000741 silica gel Substances 0.000 description 4
- 229910002027 silica gel Inorganic materials 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241000219000 Populus Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000005865 ionizing radiation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000004158 soil respiration Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3504—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing gases, e.g. multi-gas analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01V—GEOPHYSICS; GRAVITATIONAL MEASUREMENTS; DETECTING MASSES OR OBJECTS; TAGS
- G01V15/00—Tags attached to, or associated with, an object, in order to enable detection of the object
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- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Pathology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geophysics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a plant13C isotope labeling device relates to isotope labeling technical field. Including mark device, air-blower, infrared gas analysis appearance, gas absorption device, thermometer, pressure regulator, temperature regulating device and gas generating device, mark device includes three-way pipe, four-way pipe, PVC pipe, polyethylene cover bag and polyethylene film, peg graft each other through the PVC pipe between three-way pipe and the four-way pipe, the surface of three-way pipe, four-way pipe and PVC pipe all cup joints with the inside of polyethylene cover bag, the bottom fixed mounting of polyethylene cover bag has the polyethylene film. By arranging the three-way pipe, the four-way pipe and the PVC pipe, the height of the marking device is equal to three meters, plants which are planted in the field and are as high as two points and seven meters can be successfully marked, the problem that a common marking device can only mark short potted plants is solved, and the problem that the common marking device can only mark the short potted plants is expandedIs wide in13The application range of the C isotope labeling technology.
Description
Technical Field
The invention relates to the technical field of isotope labeling, in particular to a plant13C isotope labeling device.
Background
Carbon isotopes of nature including12C、13C and14c, wherein12C、13C is a stable isotope which is,14c is radioactive. The most abundant carbon isotope in nature is12C, and13c and14the abundance of C is low. Because the stable isotope has the advantages of stable physical properties, no ionizing radiation and the like, the stable isotope is stable13The C isotope technique has been widely used to study the turnover of organic carbon in soil. In the prior art, the method has the defects that,13c-isotopic labeling includes natural labeling and artificial labeling. Natural markers are typically used in field in situ trials, require replacement of C3 and C4 plants, and require relatively long replacement times (in years); the artificial marking can select proper reagent, the marking is carried out under completely controllable conditions, and the abundance of the target can be artificially regulated and controlled. The manual marking uses a suitable marking device, and the existing marking device has the following problems: limited by materials, the volume is relatively small, only short crops such as rice and wheat can be marked, and woody plants with higher height cannot be marked; limited by the structure of the device and the like, the device can only be used for marking potted plants generally, and is not suitable for in-situ marking of plants in the field. Therefore, in order to realize field in-situ marking of woody plants, a relatively large-sized plant having a simple structure, convenient assembly and transportation, and a high degree of mass is required13C isotope labeling device.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a method for processing woody plants with relatively high height13The device solves the defect that the prior device can only mark the plants with relatively low height (such as herbaceous plants), and widens the application range of the prior device13Application of C isotope labeling technology.
(II) technical scheme
In order to realize the purposes of simple structure, convenient assembly and light material marking device, the invention provides the following technical scheme: plant13The C isotope labeling device comprises a labeling device, a blower,Infrared gas analyzer, gas absorption device, thermometer, pressure regulating device, temperature regulating device and gas generating device, the mark device includes three-way pipe, four-way pipe, PVC pipe, polyethylene cover bag and polyethylene film, peg graft each other through the PVC pipe between three-way pipe and the four-way pipe, the surface of three-way pipe, four-way pipe and PVC pipe all cup joints with the inside of polyethylene cover bag, mark device's bottom fixed mounting has polyethylene film to avoid soil breathing the CO who produces2Discharging into a marking device;
the infrared gas analyzer is characterized in that a blower is fixedly arranged inside the polyethylene bag, an air outlet pipe is fixedly arranged at the output end of the blower, an infrared gas analyzer (British ADC company, EGA61 multichannel soil respiration measuring system) is fixedly arranged outside the polyethylene bag, a connecting pipe is fixedly arranged at the output end of the infrared gas analyzer, and one end, far away from the infrared gas analyzer, of the connecting pipe extends to the inside of the polyethylene bag;
the gas absorption device comprises a first air pump, the interior of the polyethylene bag is fixedly provided with the first air pump, the output end of the first air pump is fixedly provided with a first hose, the exterior of the polyethylene bag is fixedly provided with a gas washing bottle, one end of the first hose, far away from the first air pump, extends into the gas washing bottle, the interior of the gas washing bottle is fixedly provided with a second hose, one end of the second hose, far away from the gas washing bottle, extends into the polyethylene bag, and the surface of the PVC pipe is fixedly provided with a thermometer;
the pressure regulating device includes the second air pump, the inside fixed mounting of polyethylene cover bag has the second air pump, the left end of air pocket pipe has first hose air pocket pipe and second hose air pocket pipe through Y shape tee bend fixed mounting, the one end fixed connection that the air pocket pipe was kept away from to the output of second air pump and first hose air pocket pipe, the equal fixed mounting in inside of first hose air pocket pipe and second hose air pocket pipe has the valve, the outside fixed mounting of polyethylene cover bag has medical oxygen bag, the right-hand member of air pocket pipe extends to the inside of medical oxygen bag, the fixed surface of PVC pipe installs the empty box manometer.
As a preferred technical scheme of the invention, the number of the three-way pipes is four, every two three-way pipes are horizontally arranged in a group, the number of the four-way pipes is four, every two four-way pipes are horizontally arranged in a group, the four three-way pipes are respectively perpendicular to the four-way pipes in pairs, opposite surfaces of the four three-way pipes are mutually spliced through PVC pipes, opposite surfaces of the four-way pipes are mutually spliced through PVC pipes, opposite surfaces of the three-way pipes and the four-way pipes are mutually spliced through PVC pipes, PVC pipes are respectively spliced at the bottom ends of the four-way pipes, the height of the marking device is equal to three meters, and the length and the width of the marking device are respectively one meter.
As a preferable technical scheme of the invention, NaOH solution is contained in the gas washing bottle.
As a preferable technical proposal of the invention, the outside of the polyethylene bag is fixedly provided with a temperature control device, the temperature control device comprises a water tank, a heat preservation barrel, a third air pump, a spiral red copper heat exchange tube and a delivery pipe, the water tank is internally provided with water, the spiral red copper heat exchange tube is positioned below the water surface in the water tank, the heat preservation barrel wraps the whole water tank and an interlayer is arranged between the heat preservation barrel and the water tank, the output end of the third air pump extends into the water tank through the delivery pipe and is fixedly connected with the top of the spiral red copper heat exchange pipe, the bottom fixed mounting of screw-tupe red copper heat exchange tube has the conveyer pipe and the conveyer pipe one end of keeping away from screw-tupe red copper heat exchange tube extends to the inside of polyethylene cover bag, the outside of basin is provided with the intermediate layer, the interbedded outside is provided with the heat-preserving container.
As a preferred technical scheme of the invention, a gas generating device is fixedly arranged outside the polyethylene bag, the gas generating device comprises a wide-mouth bottle, a separating funnel, a first gas guide tube, a second gas guide tube, a third gas guide tube and a first air pump, a double-hole rubber plug is fixedly arranged inside the wide-mouth bottle, one end of the separating funnel is inserted into one hole of the double-hole rubber plug and extends to the inside of the wide-mouth bottle, a first gas guide tube is fixedly arranged in the other hole of the double-hole rubber plug, one end of the first gas guide tube, far away from the wide-mouth bottle, is respectively communicated with the second gas guide tube and the third gas guide tube through a Y-shaped tee joint, one end of the second gas guide tube, far away from the first gas guide tube, extends to the inside of the polyethylene bag, one end of the third gas guide tube, far away from the first gas guide tube extends to the inside of the polyethylene bag, the first air pump is located inside the polyethylene sleeve bag, the second air duct and the third air duct are both provided with check valves and valves capable of being completely closed, the check valves and the valves are used for preventing the backflow of generated gas and ensuring that the gas can only enter the polyethylene sleeve bag from the generating device.
(III) advantageous effects
Compared with the prior art, the invention provides a plant13The C isotope labeling device has the following beneficial effects:
1. the plant is13The C isotope labeling device is provided with the three-way pipe, the four-way pipe and the PVC pipe, the height of the labeling device is equal to three meters, the plants which are planted in the field and are as high as seven meters at two points can be successfully labeled, the problem that the common labeling device can only label short potted plants is solved, and the plant width is widened13Application of C isotope labeling technology.
2. The plant is13The C isotope labeling device is light in material and convenient to transport, and solves the problems of large weight and difficult transportation of the conventional large hard box body.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the gas generator of the present invention;
FIG. 3 is a schematic structural diagram of a temperature control device according to the present invention.
In the figure: 1. a marking device; 11. a three-way pipe; 12. a four-way pipe; 13. PVC pipes; 14. polyethylene bags; 15. a polyethylene film; 2. a blower; 21. an air outlet pipe; 3. an infrared gas analyzer; 31. a connecting pipe; 4. a gas absorption device; 41. a first air pump; 42. a first hose; 43. a gas washing bottle; 44. a second hose; 5. a thermometer; 6. a pressure regulating device; 61. a second air pump; 62. an air bag guide tube; 621. a first hose air bag conduit; 622. a second hose air bag conduit; 63. a medical oxygen bag; 64. an empty-box pressure gauge; 7. a temperature control device; 71. a water tank; 72. a third air pump; 73. a spiral red copper heat exchange tube; 74. a delivery pipe; 75. an electric heating tube; 76. a heat-preserving barrel; 77. an interlayer; 8. a gas generating device; 81. a jar; 82. a separating funnel; 83. a first air duct; 84. a second air duct; 85. a third air duct; 86. a first air pump.
Examples
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to FIGS. 1-3, the present invention discloses a plant13The C isotope labeling device comprises a labeling device 1, a blower 2, an infrared gas analyzer 3, a gas absorption device 4, a thermometer 5, a pressure regulating device 6, a temperature control device 7 and a gas generating device 8, wherein the labeling device 1 comprises a three-way pipe 11, a four-way pipe 12, a PVC pipe 13, a polyethylene sleeve bag 14 and a polyethylene film 15, the three-way pipe 11 and the four-way pipe 12 are mutually spliced through the PVC pipe 13, the surfaces of the three-way pipe 11, the four-way pipe 12 and the PVC pipe 13 are all sleeved with the inside of the polyethylene sleeve bag 14, and the polyethylene film 15 is fixedly installed at the bottom of the labeling device 1;
the inner part of the polyethylene bag 14 is fixedly provided with an air blower 2, the model of the air blower 2 is XGB-120, the output end of the air blower 2 is fixedly provided with an air outlet pipe 21, the outer part of the polyethylene bag 14 is fixedly provided with an infrared gas analyzer 3, the model of the infrared gas analyzer 3 is EGA61, the output end of the infrared gas analyzer 3 is fixedly provided with a connecting pipe 31(4 x 6mm, made of silica gel), and one end, far away from the infrared gas analyzer 3, of the connecting pipe 31 extends into the polyethylene bag 14;
the gas absorption device 4 comprises a first air pump 41, a first air pump 41 is fixedly installed inside the polyethylene bag set 14, a first hose 42(6 x 9mm, made of silica gel) is fixedly installed at the output end of the first air pump 41, a gas washing bottle 43 is fixedly installed outside the polyethylene bag set 14, one end, away from the first air pump 41, of the first hose 42 extends into the gas washing bottle 43, a second hose 44 is fixedly installed inside the gas washing bottle 43, one end, away from the gas washing bottle 43, of the second hose 44 extends into the polyethylene bag set 14, a thermometer 5 is fixedly installed on the surface of the PVC pipe 13, and the thermometer 5 is in the type YHZ-90191;
the pressure regulating device 6 comprises a second air pump 61, a first air pump 41 is consistent with the specification of the second air pump 61, the model of the second air pump 61 is ACO-001, the inside fixed mounting of the polyethylene bag 14 is provided with the second air pump 61, the left end of the air bag conduit 62 is provided with a first hose air bag conduit 621 and a second hose air bag conduit 622 through a Y-shaped tee joint, the output end of the second air pump 61 and the first hose air bag conduit 621 are far away from one end of the air bag conduit 62 and fixedly connected, the inside of the first hose air bag conduit 621 and the second hose air bag conduit 622 are both fixedly provided with valves, the outside fixed mounting of the polyethylene bag 14 is provided with a medical oxygen bag 63, the right end of the air bag conduit 62 extends to the inside of the medical oxygen bag 63, the surface fixed mounting of the PVC pipe 13 is provided with an air pressure gauge 64, the model of the air pressure gauge 64 is DYM3, by arranging the three-way pipe 11, the four-way pipe 12 and the PVC pipe 13, the height of the marking device 1 is equal to three meters, so that plants which are planted in the field and are as high as seven meters at two points can be successfully marked, the problem that the common marking device 1 can only mark short potted plants is solved, and the plants are widened13The application of C isotope labeling.
Specifically, the number of the three-way pipes 11 is four, every two of the three-way pipes are placed horizontally, the four-way pipes 12 are placed horizontally, every two of the four-way pipes are placed horizontally, the four three-way pipes 11 are perpendicular to the four-way pipes 12 in pairs, the opposite faces of the four-way pipes 11 are all connected with each other through PVC pipes 13 in an inserting mode, the opposite faces of the four-way pipes 12 are all connected with each other through PVC pipes 13 in an inserting mode, the opposite faces of the three-way pipes 11 and the four-way pipes 12 are connected with each other through PVC pipes 13 in an inserting mode, the bottom ends of the four-way pipes 12 are connected with PVC pipes 13 in an inserting mode, the height of the marking device 1 is equal to three meters, and the.
In the embodiment, the height of the marking device 1 is equal to three meters, so that the plants which are planted in the field and are as high as seven meters at two points can be successfully marked, and the problem that the common marking device can only mark short potted plants is solved.
Specifically, the inside of the gas washing bottle 43 contains a NaOH solution.
In this embodiment, the NaOH solution can effectively remove CO pumped into the gas washing bottle 432The marking effect of the device is improved.
Specifically, the temperature control device 7 is fixedly mounted outside the polyethylene bag 14, the temperature control device 7 includes a water tank 71, a third air pump 72(XGB-250, zhejiang tonight electromechanical limited), a spiral red copper heat exchange tube 73(19 × 1mm, red copper material), a delivery tube 74(19 × 2.5mm, PVC and steel wire composite material), an electric heating tube 75, a heat preservation barrel 76 and an interlayer 77, water is contained in the water tank 71, the electric heating tube 75 is mounted below the water surface in the water tank 71, the third air pump 72 is located inside the polyethylene bag 14, an output end of the third air pump 72 extends into the water tank 71 through the delivery tube 74 and is fixedly connected with the top of the spiral red copper heat exchange tube 73, the number of the delivery tubes 74 is two, another delivery tube 74 is fixedly mounted at the bottom of the spiral red copper heat exchange tube 73, and one end of the delivery tube 74, which is far away from the spiral red copper heat exchange tube, extends into the polyethylene bag 14, an interlayer 77 is arranged on the outer side of the water tank 71, and a heat-insulating barrel 76 is arranged on the outer side of the interlayer 77.
In this embodiment, the water tank 71 contains water, when the air temperature is low (e.g. in winter), the electric heating tube 75 below the water level in the water tank is turned on to heat to raise the water temperature, when the air temperature is high (e.g. in summer), the electric heating tube 75 is turned off and crushed ice is filled in the interlayer 77 between the heat-insulating barrel 76 and the water tank 71, the temperature of the water in the water tank 71 is adjusted according to the actual air temperature, and the temperature control device 7 can effectively adjust the temperature inside the polyethylene bag 14 in cooperation with the thermometer 5.
Specifically, the outside fixed mounting of polyethylene set bag 14 has gas generating device 8, gas generating device 8 includes wide-mouth bottle 81, separating funnel 82, first air duct 83(3 x 7mm, GG-17 borosilicate glass material), second air duct 84(6 x 9mm, silica gel material), third air duct 85(6 x 9mm, silica gel material) and first aspiration pump 86, the bottleneck department fixed mounting of wide-mouth bottle 81 has the diplopore rubber buffer, separating funnel 82 inserts a hole of diplopore rubber buffer and its lower part glass pipe extends to the inside of wide-mouth bottle 81, another hole fixed mounting of diplopore rubber buffer has first air duct 83, the one end that wide-mouth bottle 81 was kept away from to first air duct 83 is linked together with second air duct 84 and third air duct 85 respectively through Y type tee bend, the one end that first air duct 83 was kept away from to second air duct 84 extends to the inside of polyethylene set bag 14, one end of the third air duct 85, which is far away from the first air duct 83, extends to the inside of the polyethylene bag 14 and is fixedly connected with the input end of the first air pump 86, the first air pump 86 is located inside the polyethylene bag 14, and valves are installed inside the second air duct 84 and the third air duct 85. In this embodiment, open the valve on the second air duct 84 at the beginning, close the valve on the third air duct 85, let gas freely get into polyethylene bag 14, open the valve on the third air duct 85 when the reaction is close to the end, close the valve on the second air duct 84, open first aspiration pump 86 in order to form the negative pressure in first air duct 83 department, can add suitable amount of water to the wide-necked bottle 81 through separating funnel 82 simultaneously, compress the gas in the wide-necked bottle 81, make the gas in the gas generating device 8 as much as possible get into polyethylene bag 14.
The working principle and the using process of the invention are as follows: first, CO in the marking device 1 is absorbed by the gas absorption device 42The concentration is reduced, then the first hose 42 and the second hose 44 are closed, the gas washing bottle 43 is removed, the separating funnel 82, the double-hole rubber plug, the wide-mouth bottle 81, the first air duct 83, the Y-shaped tee joint, the second air duct 84, the third air duct 85 and the first air pump 86 are used for forming the gas generating device 8, and then 125ml2 mol.L is added-1To a container containing 12.50g of Ba13CO3And passing the generated gas to the marking device 1, thereby causing the gas to be generated by the gas generating device 813CO2The concentration of (D) is about 516. mu.L.L-1CO detected from EGA612The concentration was determined to be 1 hour and the labeling process was repeated 1 time, for an increase13C assimilation efficiency using unlabelled Ba12CO3Replacement of Ba13CO3Performing the same operation, repeating for 1 time, removing all devices after marking, and taking out poplar seedling from soil to obtain13C marked poplar.
In summary, the plants13The C isotope labeling device is provided with a three-way pipe 11, a four-way pipe 12 and a PVC pipe 13, the height of the labeling device 1 is equal to three meters, plants planted in the field with the height of 2.7m can be successfully labeled, the problem that a common labeling device can only label short potted plants is solved, the plants are widened13The application of C isotope labeling.
It should be noted that, in this document, terms such as "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. Plant13The C isotope labeling device comprises a labeling device (1), a blower (2), an infrared gas analyzer (3) and a gas absorption deviceDevice (4), thermometer (5), regulator (6), temperature regulating device (7) and gas generating device (8), its characterized in that: the marking device (1) comprises a three-way pipe (11), a four-way pipe (12), a PVC pipe (13), a polyethylene bag (14) and a polyethylene film (15), the three-way pipe (11) and the four-way pipe (12) are mutually inserted through the PVC pipe (13), the surfaces of the three-way pipe (11), the four-way pipe (12) and the PVC pipe (13) are all sleeved with the inside of the polyethylene bag (14), and the polyethylene film (15) is fixedly installed at the bottom of the marking device (1);
the inner part of the polyethylene bag (14) is fixedly provided with an air blower (2), the output end of the air blower (2) is fixedly provided with an air outlet pipe (21), the outer part of the polyethylene bag (14) is fixedly provided with an infrared gas analyzer (3), the output end of the infrared gas analyzer (3) is fixedly provided with a connecting pipe (31), and the input end, far away from the infrared gas analyzer (3), of the connecting pipe (31) extends to the inner part of the polyethylene bag (14);
the gas absorption device (4) comprises a first air pump (41), a first air pump (41) is fixedly installed inside the polyethylene bag (14), a first hose (42) is fixedly installed at the output end of the first air pump (41), a gas washing bottle (43) is fixedly installed outside the polyethylene bag (14), one end, away from the first air pump (41), of the first hose (42) extends into the gas washing bottle (43), a second hose (44) is fixedly installed inside the gas washing bottle (43), one end, away from the gas washing bottle (43), of the second hose (44) extends into the polyethylene bag (14), and a thermometer (5) is fixedly installed on the surface of the PVC pipe (13);
pressure regulating device (6) includes second air pump (61), the inside fixed mounting of polyethylene bagging (14) has second air pump (61), the left end of air pocket pipe (62) has first hose air pocket pipe (621) and second hose air pocket pipe (622) through Y shape tee bend fixed mounting, the output of second air pump (61) and first hose air pocket pipe (621) keep away from the one end fixed connection of air pocket pipe (62), the equal fixed mounting in inside of first hose air pocket pipe (621) and second hose air pocket pipe (622) has the valve, the outside fixed mounting of polyethylene bagging (14) has medical oxygen bag (63), the right-hand member of air pocket pipe (62) extends to the inside of medical oxygen bag (63), the fixed surface of PVC pipe (13) installs empty box manometer (64).
2. A plant according to claim 113C isotope labeling means characterized by: the number of three-way pipe (11) is four and uses per two to place for a set of front and back level, the quantity of four-way pipe (12) is four and uses per two to place for a set of front and back level, four three-way pipe (11) respectively with four-way pipe (12) are perpendicular in pairs, four three-way pipe (11) opposite face all pegs graft each other through PVC pipe (13), four the opposite face of four-way pipe (12) all pegs graft each other through PVC pipe (13), three-way pipe (11) and the opposite face of four-way pipe (12) are pegged graft each other through PVC pipe (13), four PVC pipe (13) have all been pegged graft to the bottom of four-way pipe (12), the highly equals three meters of mark device (1), length and width are one meter.
3. A plant according to claim 113C isotope labeling means characterized by: NaOH solution is contained in the gas washing bottle (43).
4. A plant according to claim 113C isotope labeling means characterized by: the outside fixed mounting of polyethylene bagging (14) has temperature regulating device (7), temperature regulating device (7) includes basin (71), third air pump (72), screw-tupe red copper heat exchange tube (73), conveyer pipe (74), electrothermal tube (75), heat-preserving container (76) and intermediate layer (77), the inside splendid attire of basin (71) has water, install electrothermal tube (75) below the surface of water of basin (71), third air pump (72) are located inside polyethylene bagging (14), the output of third air pump (72) extend to inside basin (71) and with the top fixed connection of screw-tupe red copper heat exchange tube (73) through conveyer pipe (74), the quantity of conveyer pipe (74) is two, the bottom fixed mounting of screw-tupe red copper heat exchange tube (73) has another conveyer pipe (74) and this conveyer pipe (74) keep away from the one end of screw-tupe red copper heat exchange tube and extends to the inside of polyethylene bagging (14), the water isAn interlayer (77) is arranged on the outer side of the groove (71), and a heat-insulating barrel (76) is arranged on the outer side of the interlayer (77).
5. A plant according to claim 113C isotope labeling means characterized by: the outside fixed mounting of polyethylene cover bag (14) has gas generator (8), gas generator (8) includes wide-mouth bottle (81), separating funnel (82), first air duct (83), second air duct (84), third air duct (85) and first aspiration pump (86), the bottleneck department fixed mounting of wide-mouth bottle (81) has the diplopore rubber buffer, separating funnel (82) insert a hole and the lower part glass pipe of diplopore rubber buffer and extend to the inside of wide-mouth bottle (81), another hole fixed mounting of diplopore rubber buffer has first air duct (83), the one end that wide-mouth bottle (81) were kept away from in first air duct (83) is linked together with second air duct (84) and third air duct (85) respectively through Y type tee bend, the one end that first air duct (83) were kept away from in second air duct (84) extends to the inside of polyethylene cover bag (14), one end of the third air duct (85) far away from the first air duct (83) extends to the inside of the polyethylene bag (14) and is fixedly connected with the input end of the first air pump (86), the first air pump (86) is located inside the polyethylene bag (14), and valves are installed inside the second air duct (84) and the third air duct (85).
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| CN111320158A (en) * | 2020-02-25 | 2020-06-23 | 南京林业大学 | An obtaining13C and15n double-labeling plant method and application thereof in preparation of biomass charcoal |
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