CN106980007B - Culture apparatus and measuring method for the measurement of soil organic carbon mineralization ability - Google Patents
Culture apparatus and measuring method for the measurement of soil organic carbon mineralization ability Download PDFInfo
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- CN106980007B CN106980007B CN201710137437.3A CN201710137437A CN106980007B CN 106980007 B CN106980007 B CN 106980007B CN 201710137437 A CN201710137437 A CN 201710137437A CN 106980007 B CN106980007 B CN 106980007B
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- 239000002689 soil Substances 0.000 title claims abstract description 117
- 230000033558 biomineral tissue development Effects 0.000 title claims abstract description 37
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000005259 measurement Methods 0.000 title claims abstract description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 67
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 34
- 239000007789 gas Substances 0.000 claims abstract description 24
- 239000000523 sample Substances 0.000 claims description 85
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 38
- 239000000243 solution Substances 0.000 claims description 16
- 238000010438 heat treatment Methods 0.000 claims description 13
- 238000002955 isolation Methods 0.000 claims description 7
- 229920002379 silicone rubber Polymers 0.000 claims description 4
- 238000004448 titration Methods 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 239000004016 soil organic matter Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 238000006073 displacement reaction Methods 0.000 claims description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen(.) Chemical compound [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 2
- 230000011218 segmentation Effects 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000007789 sealing Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
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- Life Sciences & Earth Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
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- General Life Sciences & Earth Sciences (AREA)
- Environmental & Geological Engineering (AREA)
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Abstract
The invention discloses a kind of culture apparatus and measuring method for the measurement of soil organic carbon mineralization ability, including incubator and nitrogen feedway, soil sample culture tube and triangular flask are provided in incubator, it is passed through nitrogen into soil sample culture tube before culture, the CO that will contain in itself in soil sample culture tube2It drains, then the soil in soil sample culture tube is cultivated, using triangular flask to the CO of release2Gas is collected, and then carries out mineralization rate measurement, and the culture apparatus and soil organic carbon mineralization ability measuring method in the present invention continuous, accurately, effectively can be measured soil organic carbon mineralization ability, and cost is relatively low.
Description
Technical field
The invention belongs to pedotheque culture technique fields, more particularly to a kind of soil organic carbon mineralization ability that is used for measure
Culture apparatus and utilize the device carry out the method for measuring of soil organic carbon mineralization ability.
Background technique
Soil organic carbon mineralization is a critical process of soil organic matter dynamic change, it refers to that soil passes through dynamic, plant
Object and microorganism are metabolized, while releasing CO2Process.This process is directly related to the release and confession of soil nutrient
It gives, is also related to the formation and discharge of greenhouse gases.It is right to control organic carbon mineralization impact factor (such as substrate, temperature, humidity)
Soil is cultivated, and is measured under respective conditions, different type, deep soil organic carbon mineralization rate, is become for establishing weather
Change, air pollution model and global carbon balance are of great significance.
Traditional soil organic carbon mineralization cultural method, " six disks delivered such as Wu Jianguo et al. at " Acta Phytoecologica Sinica "
The comparison that several Land-Uses in mountain forest area influence soil organic carbon mineralization " (2004,28 (4) 530-538) different soil
Ground Land use systems, different depth wind desiceted soil mounted in 1000ml wide-mouth bottle in, and in wide-mouth bottle place fill 0.1mol/L
The beaker of NaOH solution, to absorb the CO discharged in incubation2Gas after sealing wide-mouth bottle, is put into growth cabinet
In, 30 DEG C of temperature are controlled, after placing 1,2,4,8,18,28,38,48,68,88,130,150 and 180d respectively, takes out lye
BaCl is added2(1mol/L) solution 3ml, the CO discharged with HCl titration measuring2.Firstly, this method can not rule out in wide-mouth bottle
The CO itself contained2It is influenced caused by experiment, takes out secondly, needing repeatedly to open wide-mouth bottle during the experiment and replace NaOH
Solution can not continuously collect the CO discharged during soil incubation2, experimental implementation is cumbersome, and opens wide-mouth bottle every time and can
Certain error is caused to experiment.In addition, other most of soil organic carbon mineralization cultural methods have ignored culture and hold
The CO contained in itself in device2It is influenced caused by experimental result.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the present invention provides a kind of for soil organic matter mine
Change ability measurement culture apparatus and using the device carry out the method for measuring of soil organic carbon mineralization ability, can continuously,
Accurately, effectively soil organic carbon mineralization ability is measured.
Technical solution: in order to solve the above technical problems, provided by the present invention for the measurement of soil organic carbon mineralization ability
Culture apparatus, comprising: incubator and nitrogen feedway；Isolation board is provided in incubator, isolation board is isolated by incubator
At the gentle body collecting zone in soil sample culture region, for placing soil sample culture tube, air collection space is used in soil sample culture region
Place triangular flask；It is filled with pedotheque in the soil sample culture tube, for being cultivated soil sample sample to generate CO2Gas
Body is additionally provided with temp probe in the soil sample culture tube；The triangular flask passes through between gas-guide tube and the soil sample culture tube
It is connected to, built with NaOH solution, the CO that is discharged for collecting soil sample2Gas；It is additionally provided in the incubator
Heating device and refrigerating plant are provided with control panel outside the incubator；The nitrogen feedway is arranged in the training
The outside for supporting case, is connected to by gas-guide tube with the soil sample culture tube.
Correspondingly, the organic mineralization ability method for measuring of soil is carried out using culture apparatus the invention also discloses a kind of,
The following steps are included:
Soil sample acquisition: the pedotheque of acquisition is placed in soil sample culture tube, and by the top end opening of soil sample culture tube, the
One air guide interface tube and the second air guide interface tube are sealed, and then the pedotheque being collected is put into incubator；
CO2Nitrogen injection before gas collection: the first air guide interface tube and nitrogen of gas-guide tube connection soil sample culture tube are utilized
Air feed system, the air in nitrogen displacement soil sample culture tube being passed through using nitrogen feedway；
Temperature control: starting heating device and refrigerating plant carry out Mineralized Culture to pedotheque；
CO2Gas collection: being connected to the second air guide interface tube and triangular flask of soil sample culture tube using gas-guide tube, triangular flask
NaOH solution absorbs the CO discharged during soil organic carbon mineralization2；
Mineralization rate measurement: the CO of NaOH solution absorption is measured with HCl titration2, finally CO2Cumulative release amount conversion
For mineralization rate.
The utility model has the advantages that the culture apparatus in the present invention for the measurement of soil organic carbon mineralization ability is received equipped with individual gas
Collect region, for collecting CO2Triangular flask can substitute at any time, and then may be implemented continuously to collect in the same soil sample incubation
The CO released2；Equipped with nitrogen feedway, it is passed through nitrogen into soil sample culture tube before culture, it will be in soil sample culture tube
The CO itself contained2It drains, is passed through nitrogen into soil sample culture tube again after culture, the CO that will be generated in mineralization process2Gas
Body is all pushed into gas collection unit, to further reduce experimental error；Incubator uses plastic material, and price is low
It is honest and clean；It is equipped with insulating inside incubator, and is provided with temperature control zone, can effectively control temperature required for soil sample culture
Degree condition.The organic mineralization ability measuring method of soil in the present invention, using above-mentioned culture apparatus, to soil sample culture before culture
Nitrogen is passed through in pipe, the CO that will contain in itself in soil sample culture tube2It drains；Further, it is trained again to soil sample after culture
It supports in pipe and is passed through nitrogen, the CO that will be generated in mineralization process2Gas is all pushed into triangular flask, to further reduce experiment
Error；In addition, replacing rubber ring to carry out uniformly smearing sealing to each interface of device using vacuum silicon rubber, system is effectively guaranteed
Air-tightness.
Detailed description of the invention
Fig. 1 is the structural schematic diagram in the present invention for the culture apparatus of soil organic carbon mineralization ability measurement；
Fig. 2 is the structural schematic diagram of soil sample culture tube；
In Fig. 1,1 is incubator, and 2 be isolation board, and 3 be soil sample culture tube, and 4 be triangular flask, and 5 be heating tube, and 6 be electric wind
Fan, 7 be gas flow optimized valve, and 8 be flowmeter, and 9 be nitrogen feedway, and 10 be control panel；11 be temp probe；In Fig. 2,
31 be the top end opening of soil sample culture tube, and 32 be the first air guide interface tube of soil sample culture tube, and 33 be the second of soil sample culture tube
Air guide interface tube.
Specific embodiment
To keep purpose and the technical solution of the embodiment of the present invention clearer, below in conjunction with the attached of the embodiment of the present invention
Figure, is clearly and completely described the technical solution of the embodiment of the present invention.
As shown in Figure 1, the culture apparatus for the measurement of soil organic carbon mineralization ability includes: incubator 1 and nitrogen supply
Device 9；Isolation board 2 is provided in incubator 1, incubator 1 is isolated into two regions by isolation board 2, and a region is known as soil
Sample culture region, for placing soil sample culture tube 3, another region is known as air collection space, for placing triangular flask 4, soil
Sample culture tube 3 is correspondingly arranged with triangular flask 4, i.e. the corresponding triangular flask 4 of a soil sample culture tube 3；It is loaded in soil sample culture tube 3
There is pedotheque, for being cultivated soil sample sample to generate CO2Gas is additionally provided with temp probe in soil sample culture tube 3
11；Triangular flask 4 between gas-guide tube and soil sample culture tube 3 by being connected to, built with excessive NaOH solution, for collecting
The CO that soil sample is discharged2Gas；It is additionally provided with heating device 5 and refrigerating plant 6 in incubator 1, is arranged outside incubator 1
There is control panel 10, be electrically connected between control panel 10 and heating device 5 and refrigerating plant 6, convenient for 5 He of heating device
Refrigerating plant 6 is controlled；The outside of incubator 1 is arranged in nitrogen feedway 9, is connected by gas-guide tube and soil sample culture tube 3
It is logical, nitrogen is passed through for soil sample culture tube 3.
Incubator 1 uses closed structure, a length of 50cm, width 40m, a height of 50cm, be provided in the cabinet of incubator 1 every
Hot insulating layer, such as cabinet can use double-layer structure, and cabinet outer body material is plastics, and internal layer uses common heat-insulated guarantor
Adiabator, such as polyethylene foam sheet (EPS), the connection of gap between inside and outside two layers for routes such as electric fan, heating tubes.Incubator
Culturing rack is equipped in 1 can place multiple soil sample cultures by soil sample culture region segmentation at several soil sample culture units simultaneously
Pipe, the height of soil sample culture tube 3 are if that 15cm, diameter 10cm can choose place multiple soil sample culture tubes 3 at one
Temp probe 11 is set in soil sample culture tube 3 to carry out temperature acquisition, reduces cost.
As shown in figs. 1 and 2, soil sample culture tube 3 is organic glass material, using the tubular structure of upper end opening, one
Side wall lower ends are provided with the first air guide interface tube 32, and another sidewall upper opposite with the side wall is provided with the second air guide interface tube
33, the first air guide interface tube 32 is connected to by gas-guide tube with nitrogen feedway 9, is additionally provided with gas flow optimized on the gas-guide tube
Valve 7 and flowmeter 8；Second air guide interface tube 33 is connected to by gas-guide tube with triangular flask 4.
Above-mentioned nitrogen feedway 9 can use steel cylinder, and heating device 5 can use heating tube, and refrigerating plant 6 can be adopted
Use electric fan.Heating device 5 can use immerging metal tube heater, and respectively set one at the position up and down of 1 cabinet of incubator, make to cultivate
Soil sample culture tube 3 in case 1 can be heated evenly；Refrigerating plant 6 can use two small electric fans, be placed in the left and right of bottom
Both ends.
When being measured using the organic mineralization ability of culture apparatus progress soil in Fig. 1, comprising the following steps:
Soil sample acquisition: representative pedotheque (number of samples is taken at random in soil region to be determined with sample acquisition machine
Depending on testing specific requirement), pedotheque is placed in soil sample culture tube 3, and by the top end opening of soil sample culture tube 31,
One air guide interface tube 32 and the second air guide interface tube 33 are sealed, and then the sample being collected is put into incubator 1；
Nitrogen injection: being connected to the first air guide interface tube 3.2 and nitrogen feedway 9 of soil sample culture tube 3 using gas-guide tube,
And air guide interface tube is uniformly smeared with vacuum silicon rubber and plays sealing function, gas flow optimized valve 7 is then opened, then open nitrogen
The switch of feedway 9, is at the uniform velocity passed through nitrogen gas, carries out flow control by flowmeter 8, the gas volume for being passed through nitrogen is big
In or be equal to soil incubation pipe in be added soil sample after remaining volume, air in each culture tube is drained with this, remove it is original
CO2The interference of gas；Using the second air guide interface tube and triangular flask 4 of gas-guide tube connection soil sample culture tube 3, and use vacuum silicon rubber
Air guide interface tube is uniformly smeared and plays sealing function；
Temperature control: start the heating device 5 and refrigerating plant 6 of the present apparatus, and observe temp probe in soil sample culture tube
11 reading adjusts control panel 10, the temperature needed for the temperature of temperature control zone reaches culture, in the case, right
Pedotheque carries out Mineralized Culture；
CO2Gas collection: the CO discharged during soil organic carbon mineralization is absorbed using the NaOH solution of triangular flask 42；
Mineralization rate measurement: the CO of NaOH solution absorption is measured with HCl titration2, finally CO2Cumulative release amount conversion
For mineralization rate.
In order to further increase experimental error, in CO2After gas collection step, can also in soil sample culture tube again
Secondary to be passed through nitrogen, be passed through the volume of nitrogen is advisable with being equal to or slightly less than soil sample culture pipe volume, in order to will be in mineralization process
The CO of generation2Gas is all in push-in triangular flask.
When carrying out the organic mineralization ability of soil using the culture apparatus in the present invention and measuring, it is only necessary to according to experiment need by
Triangular flask is replaced, and needs repeatedly to open wide-mouth bottle compared to the measurement device introduced in background technique to replace NaOH
Solution causes CO2Gas loss can continuously collect the CO discharged during soil incubation2.In addition, passing through control panel pair
Incubator carries out temperature control, convenient for operation, relative to constant incubator, and reduces testing cost.
Claims (8)
1. a kind of culture apparatus for the measurement of soil organic carbon mineralization ability, which is characterized in that the device includes: incubator
(1) and nitrogen feedway (9)；It is provided with isolation board (2) in incubator (1), incubator (1) is isolated by isolation board (2)
The gentle body collecting zone in soil sample culture region, for placing soil sample culture tube (3), air collection space is used in soil sample culture region
It places triangular flask (4)；It is filled with pedotheque in the soil sample culture tube (3), is additionally provided with temperature in the soil sample culture tube (3)
Degree probe (11)；The triangular flask (4) passes through to be connected between gas-guide tube and the soil sample culture tube (3), built with
NaOH solution；Heating device (5) and refrigerating plant (6) are additionally provided in the incubator (1), the incubator (1) outside
It is provided with control panel (10)；The nitrogen feedway (9) setting in the outside of the incubator (1), by gas-guide tube with
Soil sample culture tube (3) connection.
2. culture apparatus according to claim 1, which is characterized in that the soil sample culture tube (3) is using upper end opening
Tubular structure, one side wall lower ends are provided with the first air guide interface tube (32), another sidewall upper setting opposite with the side wall
There is the second air guide interface tube (33), the first air guide interface tube (32) is connected by gas-guide tube and the nitrogen feedway (9)
It is logical；The second air guide interface tube (33) is connected to by gas-guide tube with the triangular flask (4).
3. culture apparatus according to claim 2, which is characterized in that in the first air guide interface tube (32) and the nitrogen
Control damper (7) and flowmeter (8) are additionally provided on gas-guide tube between air feed system (9).
4. culture apparatus as claimed in any of claims 1 to 3, which is characterized in that the cabinet of the incubator (1)
Inside it is provided with insulating.
5. culture apparatus as claimed in any of claims 1 to 3, which is characterized in that also set in the incubator (1)
It is equipped with culturing rack, by the soil sample culture region segmentation at several soil sample culture units, for placing the soil sample culture tube
(3)。
6. a kind of carry out the method for measuring of soil organic carbon mineralization ability, feature using culture apparatus as claimed in claim 2
It is, comprising the following steps:
Soil sample acquisition: the pedotheque of acquisition is placed in soil sample culture tube, and the top end opening of soil sample culture tube, first are led
Air pipe interface and the second air guide interface tube are sealed, and then the pedotheque being collected is put into incubator；
CO2Nitrogen injection before gas collection: it is supplied using the first air guide interface tube and nitrogen of gas-guide tube connection soil sample culture tube
Device, the air in nitrogen displacement soil sample culture tube being passed through using nitrogen feedway；
Temperature control: starting heating device and refrigerating plant carry out Mineralized Culture to pedotheque；CO2Gas collection: using leading
Tracheae is connected to the second air guide interface tube and triangular flask of soil sample culture tube, and the NaOH solution in triangular flask absorbs soil organic matter mine
The CO discharged during change2；
Mineralization rate measurement: the CO of NaOH solution absorption is measured with HCl titration2, finally CO2Cumulative release amount is converted into mine
Change rate.
7. soil organic carbon mineralization ability method for measuring according to claim 6, which is characterized in that in the CO2Gas
After collection step, further include the steps that again to being passed through nitrogen again in soil sample culture tube.
8. soil organic carbon mineralization ability method for measuring according to claim 6 or 7, which is characterized in that trained to soil sample
It supports when pipe air guide interface tube is sealed and uses vacuum silicon rubber.
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| CN201710137437.3A CN106980007B (en) | 2017-03-09 | 2017-03-09 | Culture apparatus and measuring method for the measurement of soil organic carbon mineralization ability |
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| CN201710137437.3A CN106980007B (en) | 2017-03-09 | 2017-03-09 | Culture apparatus and measuring method for the measurement of soil organic carbon mineralization ability |
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| CN106980007B true CN106980007B (en) | 2019-04-30 |
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| CN108362825A (en) * | 2018-05-17 | 2018-08-03 | 中国农业科学院农田灌溉研究所 | A kind of interior soil organic carbon mineralization culture monitoring device |
| CN111812089A (en) * | 2020-07-24 | 2020-10-23 | 北京林业大学 | Mineralization of CO by organic carbon in soil2Device and method for continuously measuring release amount |
| CN112198299B (en) * | 2020-09-29 | 2023-07-04 | 南京林业大学 | Method for measuring mineralization capacity of organic carbon in soil based on Bayes theory |
| CN113444612B (en) * | 2021-05-20 | 2022-12-13 | 北京工业大学 | Microcosmic culture device and application thereof in quantitative analysis of soil carbon diffusion and microorganism utilization process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH11337484A (en) * | 1998-03-26 | 1999-12-10 | Kubota Corp | Soil analyzing method, spectroscopic analyser and agricultural working vehicle loaded with spectroscopic analyser |
| DE102012008584B3 (en) * | 2012-04-27 | 2013-06-13 | Falko Kuhnke | Device for analyzing small-volume soil gas samples to determine concentration of carbon dioxide, has stamper for occupying internal diameter of probe such that dead volume is minimized compared to actual sample volume |
| CN104111319B (en) * | 2014-07-29 | 2016-06-29 | 东北农业大学 | Can the vertical soil mineralization rate determination device of inducing QI cylinder |
| CN205404505U (en) * | 2016-03-04 | 2016-07-27 | 湖南农业大学 | Soil gas collecting device |
| CN106018025B (en) * | 2016-05-25 | 2019-03-05 | 河海大学 | A kind of auto-manual system culture apparatus for the measurement of soil denitrification potentiality |
| CN205941537U (en) * | 2016-06-13 | 2017-02-08 | 湖北省农业科学院粮食作物研究所 | Organic carbon mineralize mineralization of formula of ventilating in succession soil survey device |
| CN106442066B (en) * | 2016-11-09 | 2023-10-13 | 中国农业科学院农业资源与农业区划研究所 | Soil culture device with small gas collector |
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