CN106980007A - The culture apparatus and assay method determined for soil organic carbon mineralization ability - Google Patents
The culture apparatus and assay method determined for soil organic carbon mineralization ability Download PDFInfo
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- CN106980007A CN106980007A CN201710137437.3A CN201710137437A CN106980007A CN 106980007 A CN106980007 A CN 106980007A CN 201710137437 A CN201710137437 A CN 201710137437A CN 106980007 A CN106980007 A CN 106980007A
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- 239000002689 soil Substances 0.000 title claims abstract description 118
- 230000033558 biomineral tissue development Effects 0.000 title claims abstract description 37
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 24
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 24
- 238000003556 assay Methods 0.000 title abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 67
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 34
- 239000007789 gas Substances 0.000 claims abstract description 24
- 239000000523 sample Substances 0.000 claims description 85
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 13
- 229920002379 silicone rubber Polymers 0.000 claims description 4
- 238000004448 titration Methods 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 238000006073 displacement reaction Methods 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen(.) Chemical compound [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 2
- 230000011218 segmentation Effects 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000004016 soil organic matter Substances 0.000 description 2
- 229920005479 Lucite® Polymers 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 1
- 229910001626 barium chloride Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Geology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Remote Sensing (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of culture apparatus and assay method determined for soil organic carbon mineralization ability, including incubator and nitrogen feedway, soil sample culture tube and triangular flask are provided with incubator, nitrogen is passed through into soil sample culture tube before culture, the CO that will in itself contain in soil sample culture tube2Drain, then the soil in soil sample culture tube is cultivated, utilize CO of the triangular flask to release2Gas is collected, and the culture apparatus and soil organic carbon mineralization ability assay method then carried out in mineralization rate measure, the present invention continuously, accurately, effectively can be measured to soil organic carbon mineralization ability, and cost is relatively low.
Description
Technical field
Determined the invention belongs to pedotheque culture technique field, more particularly to a kind of soil organic carbon mineralization ability that is used for
Culture apparatus and utilize the device carry out soil organic carbon mineralization ability method for measuring.
Background technology
Soil organic carbon mineralization is a critical process of soil organic matter dynamic change, and it refers to that soil passes through dynamic, plant
Thing and microorganism are metabolized, while discharging CO2Process.This process is directly connected to the release and confession of soil nutrient
Give, be also related to the formation and discharge of greenhouse gases.Control organic carbon mineralization factor of influence (such as substrate, temperature, humidity) right
Soil is cultivated, and is determined under respective conditions, different type, deep soil organic carbon mineralization speed, is become for setting up weather
Change, air pollution model and global carbon balance are significant.
Traditional soil organic carbon mineralization cultural method, such as Wu Jianguo et al. exists《Acta Phytoecologica Sinica》Deliver《Six disks
The comparison that several Land-Uses in mountain forest area influence on soil organic carbon mineralization》(2004,28 (4) 530-538) is different soil
Ground Land use systems, the wind desiceted soil of different depth are mounted in 1000ml wide-mouth bottle, and placement fills 0.1mol/L in wide-mouth bottle
The beaker of NaOH solution, the CO discharged to absorb in incubation2Gas, after wide-mouth bottle is sealed, is put into growth cabinet
In, 30 DEG C of temperature is controlled, after 1,2,4,8,18,28,38,48,68,88,130,150 and 180d is placed respectively, alkali lye is taken out
Add BaCl2(1mol/L) solution 3ml, the CO discharged with HCl titration measurings2.First, this method can not be excluded in wide-mouth bottle
The CO itself contained2To the influence that causes of experiment, secondly, need repeatedly to open wide-mouth bottle in experimentation and take out and change NaOH
Solution, it is impossible to continuous to collect the CO discharged during soil incubation2, experimental implementation is cumbersome, and opening wide-mouth bottle can every time
Certain error is caused to experiment.Hold in addition, other most soil organic carbon mineralization cultural methods have ignored culture
The CO contained in itself in device2The influence caused to experimental result.
The content of the invention
Goal of the invention：In order to overcome the deficiencies in the prior art, the present invention provides a kind of for soil organic matter ore deposit
Change ability determine culture apparatus and using the device progress soil organic carbon mineralization ability method for measuring, can continuously,
Accurately, effectively soil organic carbon mineralization ability is measured.
Technical scheme：In order to solve the above technical problems, determined provided by the present invention for soil organic carbon mineralization ability
Culture apparatus, including：Incubator and nitrogen feedway；Division board is provided with incubator, division board isolates incubator
Into the gentle body collecting zone in soil sample culture region, soil sample culture region is used to place soil sample culture tube, and air collection space is used for
Place triangular flask；Pedotheque is filled with the soil sample culture tube, for being cultivated soil sample sample to produce CO2Gas
Temp probe is additionally provided with body, the soil sample culture tube；The triangular flask passes through between airway tube and the soil sample culture tube
Connected, built with NaOH solution, for collecting the CO that soil sample is discharged2Gas；It is additionally provided with the incubator
Heater and refrigerating plant, control panel is provided with outside the incubator；The nitrogen feedway is arranged on the training
The outside of case is supported, is connected by airway tube with the soil sample culture tube.
Accordingly, the invention also discloses a kind of using the culture apparatus progress organic mineralization ability method for measuring of soil,
Comprise the following steps：
Soil sample is gathered：The pedotheque of collection is placed in soil sample culture tube, and by the top end opening of soil sample culture tube,
One air guide interface tube and the second air guide interface tube are sealed, and then the pedotheque being collected is put into incubator；
CO2Nitrogen injection before gas collection：The the first air guide interface tube and nitrogen of soil sample culture tube are connected using airway tube
Air in air feed system, the nitrogen displacement soil sample culture tube being passed through using nitrogen feedway；
Temperature control：Start heater and refrigerating plant, Mineralized Culture is carried out to pedotheque；
CO2Gas collection：The the second air guide interface tube and triangular flask of soil sample culture tube are connected using airway tube, triangular flask
NaOH solution absorbs the CO discharged during soil organic carbon mineralization2；
Mineralization rate is determined：The CO of NaOH solution absorption is determined with HCl titrations2, finally CO2Cumulative release amount is converted
For mineralization rate.
Beneficial effect：The culture apparatus for being used for soil organic carbon mineralization ability measure in the present invention is received provided with single gas
Collect region, for collecting CO2Triangular flask can substitute at any time, and then can realize and continuously collect in same soil sample incubation
The CO discharged2；Provided with nitrogen feedway, nitrogen is passed through into soil sample culture tube before culture, by soil sample culture tube
The CO itself contained2Drain, nitrogen is being passed through into soil sample culture tube again after culture terminates, by the CO produced in mineralization process2Gas
Body is all pushed into gas collection unit, so as to further reduce experimental error；Incubator uses plastic material, and price is low
It is honest and clean；Insulating is provided with inside incubator, and is provided with temperature control zone, the temperature required for soil sample culture can be effectively controlled
Degree condition.The organic mineralization ability assay method of soil in the present invention, using above-mentioned culture apparatus, to soil sample culture before culture
Nitrogen is passed through in pipe, the CO that will in itself contain in soil sample culture tube2Drain；Further, trained again to soil sample after culture terminates
Support in pipe and be passed through nitrogen, by the CO produced in mineralization process2Gas is all pushed into triangular flask, so as to further reduce experiment
Error；Seal in addition, replacing rubber ring to carry out uniform smear to each interface of device using vacuum silicon rubber, be effectively guaranteed system
Air-tightness.
Brief description of the drawings
Fig. 1 is to be used for the structural representation for the culture apparatus that soil organic carbon mineralization ability is determined in the present invention；
Fig. 2 is the structural representation of soil sample culture tube；
In Fig. 1,1 is incubator, and 2 be division board, and 3 be soil sample culture tube, and 4 be triangular flask, and 5 be heating tube, and 6 be electric wind
Fan, 7 be gas flow optimized valve, and 8 be flowmeter, and 9 be nitrogen feedway, and 10 be control panel；11 be temp probe；In Fig. 2,
31 be the top end opening of soil sample culture tube, and 32 be the first air guide interface tube of soil sample culture tube, and 33 be the second of soil sample culture tube
Air guide interface tube.
Embodiment
To make the purpose and technical scheme of the embodiment of the present invention clearer, below in conjunction with the attached of the embodiment of the present invention
Figure, the technical scheme to the embodiment of the present invention is clearly and completely described.
As shown in figure 1, including for the culture apparatus that soil organic carbon mineralization ability is determined：Incubator 1 and nitrogen supply
Device 9；Division board 2 is provided with incubator 1, incubator 1 is isolated into two regions by division board 2, and a region is referred to as soil
Sample culture region, for placing soil sample culture tube 3, another region is referred to as air collection space, for placing triangular flask 4, soil
Sample culture tube 3 is correspondingly arranged with triangular flask 4, i.e., one one triangular flask 4 of correspondence of soil sample culture tube 3；Loaded in soil sample culture tube 3
There is pedotheque, for being cultivated soil sample sample to produce CO2Temp probe is additionally provided with gas, soil sample culture tube 3
11；Triangular flask 4 between airway tube and soil sample culture tube 3 by being connected, built with excessive NaOH solution, for collecting
The CO that soil sample is discharged2Gas；Heater 5 and refrigerating plant 6 are additionally provided with incubator 1, is set outside incubator 1
There is control panel 10, be electrically connected between control panel 10 and heater 5 and refrigerating plant 6, is easy to the He of heater 5
Refrigerating plant 6 is controlled；Nitrogen feedway 9 is arranged on the outside of incubator 1, is connected by airway tube with soil sample culture tube 3
It is logical, it is that soil sample culture tube 3 is passed through nitrogen.
Incubator 1 use be provided with closed structure, a length of 50cm, a width of 40m, a height of 50cm, the casing of incubator 1 every
Hot heat-insulation layer, such as casing can use double-decker, and casing outer body material is plastics, and internal layer uses common heat-insulated guarantor
Adiabator, such as polyethylene foam sheet (EPS), the gap between inside and outside two layers powers the connections of the circuits such as fan, heating tube.Incubator
Culturing rack is provided with 1, by soil sample culture region segmentation into several soil sample culture units, multiple soil sample cultures can be placed simultaneously
Pipe, the height of soil sample culture tube 3 is 15cm, a diameter of 10cm, if place multiple soil sample culture tubes 3, can be selected at one
Set temp probe 11 to carry out temperature acquisition in soil sample culture tube 3, reduce cost.
As shown in figs. 1 and 2, soil sample culture tube 3 is lucite material, using the tubular structure of upper end open, one
Side wall lower ends are provided with the first air guide interface tube 32, and another sidewall upper relative with the side wall is provided with the second air guide interface tube
33, the first air guide interface tube 32 is connected by airway tube with nitrogen feedway 9, and gas flow optimized is additionally provided with the airway tube
Valve 7 and flowmeter 8；Second air guide interface tube 33 is connected by airway tube with triangular flask 4.
Above-mentioned nitrogen feedway 9 can use steel cylinder, and heater 5 can use heating tube, and refrigerating plant 6 can be adopted
Use electric fan.Heater 5 can use immerging metal tube heater, and respectively set one at the position up and down of the casing of incubator 1, make culture
Soil sample culture tube 3 in case 1 can be heated evenly；Refrigerating plant 6 can be placed in the left and right of bottom using two small electric fans
Two ends.
When being determined using the organic mineralization ability of culture apparatus progress soil in Fig. 1, comprise the following steps：
Soil sample is gathered：Representative pedotheque (number of samples is taken at random in soil region to be determined with sample acquisition machine
Depending on experiment specific requirement), pedotheque is placed in soil sample culture tube 3, and by the top end opening 31 of soil sample culture tube, the
One air guide interface tube 32 and the second air guide interface tube 33 are sealed, and then the sample being collected is put into incubator 1；
Nitrogen injects：The the first air guide interface tube 3.2 and nitrogen feedway 9 of soil sample culture tube 3 are connected using airway tube,
And with vacuum silicon rubber, to air guide interface tube, uniformly smearing plays sealing function, then opens gas flow optimized valve 7, then open nitrogen
The switch of feedway 9, is at the uniform velocity passed through nitrogen gas, and flow control is carried out by flowmeter 8, and the gas volume for being passed through nitrogen is big
In or equal to add remaining volume after soil sample in soil incubation pipe, air in each culture tube is drained with this, removed original
CO2The interference of gas；The the second air guide interface tube and triangular flask 4 of soil sample culture tube 3 are connected using airway tube, and uses vacuum silicon rubber
Air guide interface tube is uniformly smeared and plays sealing function；
Temperature control：Start the heater 5 and refrigerating plant 6 of the present apparatus, and observe temp probe in soil sample culture tube
11 reading, adjusts control panel 10, the temperature needed for the temperature of temperature control zone reaches culture, in the case, right
Pedotheque carries out Mineralized Culture；
CO2Gas collection：The CO discharged during soil organic carbon mineralization is absorbed using the NaOH solution of triangular flask 42；
Mineralization rate is determined：The CO of NaOH solution absorption is determined with HCl titrations2, finally CO2Cumulative release amount is converted
For mineralization rate.
In order to further improve experimental error, in CO2After gas collection step terminates, can also in soil sample culture tube again
It is secondary to be passed through nitrogen, the volume of nitrogen is passed through to be advisable equal to or slightly less than soil sample culture pipe volume, in order to by mineralization process
The CO of generation2Gas is all in push-in triangular flask.
When carrying out the organic mineralization ability of soil using the culture apparatus in the present invention and determining, it is only necessary to according to experiment need by
Triangular flask is replaced, and needs repeatedly to open wide-mouth bottle compared to the measure device introduced in background technology to replace NaOH
Solution causes CO2Gas loss, can continuously collect the CO discharged during soil incubation2.In addition, passing through control panel pair
Incubator carries out temperature control, is easy to operation, relative to constant incubator, testing cost is reduced again.
Claims (8)
1. a kind of culture apparatus determined for soil organic carbon mineralization ability, it is characterised in that the device includes：Incubator
（1）With nitrogen feedway（9）；In incubator（1）Inside it is provided with division board（2）, division board（2）By incubator（1）It is isolated into
The gentle body collecting zone in soil sample culture region, soil sample culture region is used to place soil sample culture tube（3）, air collection space is used for
Place triangular flask（4）；The soil sample culture tube（3）Inside it is filled with pedotheque, the soil sample culture tube（3）Inside it is additionally provided with temperature
Degree probe（11）；The triangular flask（4）Pass through airway tube and the soil sample culture tube（3）Between connected, built with
NaOH solution；In the incubator（1）Inside it is additionally provided with heater（5）And refrigerating plant（6）, in the incubator（1）Outside
It is provided with control panel（10）；The nitrogen feedway（9）It is arranged on the incubator（1）Outside, by airway tube with
The soil sample culture tube（3）Connection.
2. culture apparatus according to claim 1, it is characterised in that the soil sample culture tube（3）Using upper end open
Tubular structure, one side wall lower ends are provided with the first air guide interface tube（32）, another sidewall upper relative with the side wall set
There is the second air guide interface tube（33）, the first air guide interface tube（32）Pass through airway tube and the nitrogen feedway（9）Even
It is logical；The second air guide interface tube（33）Pass through airway tube and the triangular flask（4）Connection.
3. culture apparatus according to claim 2, it is characterised in that in the first air guide interface tube（32）With the nitrogen
Air feed system（9）Between airway tube on be additionally provided with control damper（7）And flowmeter（8）.
4. culture apparatus as claimed in any of claims 1 to 3, it is characterised in that the incubator（1）Casing
Inside it is provided with insulating.
5. culture apparatus as claimed in any of claims 1 to 3, it is characterised in that the incubator（1）Inside also set
Culturing rack is equipped with, by the soil sample culture region segmentation into several soil sample culture units, for placing the soil sample culture tube
（3）.
6. a kind of culture apparatus using described in claim 1 carries out the organic mineralization ability method for measuring of soil, its feature exists
In comprising the following steps：
Soil sample is gathered：The pedotheque of collection is placed in soil sample culture tube, and the top end opening of soil sample culture tube, first are led
Air pipe interface and the second air guide interface tube are sealed, and then the pedotheque being collected is put into incubator；
CO2Nitrogen injection before gas collection：The first air guide interface tube and the nitrogen supply of soil sample culture tube are connected using airway tube
Air in device, the nitrogen displacement soil sample culture tube being passed through using nitrogen feedway；
Temperature control：Start heater and refrigerating plant, Mineralized Culture is carried out to pedotheque；
CO2Gas collection：Connected using airway tube in the second air guide interface tube and triangular flask of soil sample culture tube, triangular flask
NaOH solution absorbs the CO discharged during soil organic carbon mineralization2；
Mineralization rate is determined：The CO of NaOH solution absorption is determined with HCl titrations2, finally CO2Cumulative release amount is converted into ore deposit
Change speed.
7. the organic mineralization ability method for measuring of soil according to claim 6, it is characterised in that in the CO2Gas is received
Collection step terminate after, in addition to again to being passed through nitrogen again in soil sample culture tube the step of.
8. the organic mineralization ability method for measuring of soil according to claim 6 or 7, it is characterised in that to soil sample culture
Pipe air guide interface tube employs vacuum silicon rubber when being sealed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710137437.3A CN106980007B (en) | 2017-03-09 | 2017-03-09 | Culture apparatus and measuring method for the measurement of soil organic carbon mineralization ability |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710137437.3A CN106980007B (en) | 2017-03-09 | 2017-03-09 | Culture apparatus and measuring method for the measurement of soil organic carbon mineralization ability |
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| CN106980007A true CN106980007A (en) | 2017-07-25 |
| CN106980007B CN106980007B (en) | 2019-04-30 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108362825A (en) * | 2018-05-17 | 2018-08-03 | 中国农业科学院农田灌溉研究所 | A kind of interior soil organic carbon mineralization culture monitoring device |
| CN111812089A (en) * | 2020-07-24 | 2020-10-23 | 北京林业大学 | Mineralization of CO by organic carbon in soil2Device and method for continuously measuring release amount |
| CN112198299A (en) * | 2020-09-29 | 2021-01-08 | 南京林业大学 | Method for measuring soil organic carbon mineralization capacity based on Bayesian theory |
| CN113444612A (en) * | 2021-05-20 | 2021-09-28 | 北京工业大学 | Microcosmic culture device and application thereof in quantitative analysis of soil carbon diffusion and microorganism utilization process |
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| CN111812089A (en) * | 2020-07-24 | 2020-10-23 | 北京林业大学 | Mineralization of CO by organic carbon in soil2Device and method for continuously measuring release amount |
| CN112198299A (en) * | 2020-09-29 | 2021-01-08 | 南京林业大学 | Method for measuring soil organic carbon mineralization capacity based on Bayesian theory |
| CN113444612A (en) * | 2021-05-20 | 2021-09-28 | 北京工业大学 | Microcosmic culture device and application thereof in quantitative analysis of soil carbon diffusion and microorganism utilization process |
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| Publication number | Publication date |
|---|---|
| CN106980007B (en) | 2019-04-30 |
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