CN108362825A - A kind of interior soil organic carbon mineralization culture monitoring device - Google Patents

A kind of interior soil organic carbon mineralization culture monitoring device Download PDF

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Publication number
CN108362825A
CN108362825A CN201810473621.XA CN201810473621A CN108362825A CN 108362825 A CN108362825 A CN 108362825A CN 201810473621 A CN201810473621 A CN 201810473621A CN 108362825 A CN108362825 A CN 108362825A
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China
Prior art keywords
temperature
culture
bottle
organic carbon
rubber
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CN201810473621.XA
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Chinese (zh)
Inventor
张笑培
周新国
王和洲
杨慎骄
刘安能
陈金平
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Farmland Irrigation Research Institute of CAAS
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Farmland Irrigation Research Institute of CAAS
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Priority to CN201810473621.XA priority Critical patent/CN108362825A/en
Publication of CN108362825A publication Critical patent/CN108362825A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/16Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of indoor soil organic carbon mineralization culture monitoring device, which includes thermostatic water bath, temperature securing component, culture bottle, absorption bottle, conduit, and the tube at one end passes through plug in first rubber to extend to inside the culture bottle;The other end passes through plug in second rubber to extend to sodium hydroxide liquid levels or less.Device provided by the present application controls cultivation temperature using the thermostatic water bath of temperature control box control, specific temperature condition can be provided for soil organic carbon mineralization.Culture bottle and absorption bottle separate, and are connected by conduit, can be with METHOD FOR CONTINUOUS DETERMINATION soil organic carbon mineralization rate and accumulation mineralising amount.Culture bottle holds the soil sample incubator of soil and claims have the beaker of certain water to be put into bottle together, plug sealing, simple in structure in blend rubber, easy to operate, it is at low cost, efficiently solve the problems, such as that the inconvenience of existing technologies operation, equipment cost is high, the inconvenience such as complicated.

Description

A kind of interior soil organic carbon mineralization culture monitoring device
Technical field
The present invention relates to atmospheric monitoring technical fields in soil, more particularly to a kind of indoor soil organic carbon mineralization culture Monitoring device.
Background technology
Carbon in soil is discharged to air, is mainly acted on by organic carbon mineralization, is entered air from pedosphere in the form of CO2 Circle.Soil organic carbon mineralization is a critical process of soil organic matter dynamic change, mainly utilized by edaphon, It decomposes active organic carbon in soil and completes the metabolic cycles of itself, while releasing the process of CO2, direct relation Soil Trace Gases Formation and Soil organic carbon pool storage.Disclose soil organic carbon mineralization rule, for carbon base in soil scientific management and Effective control of Global climate change etc. has highly important practice significance.
Currently, studying the more advanced method of soil organic carbon mineralization has gas chromatography and infrared ray gas analysis method. Gas chromatography, which is primarily adapted for use in, measures small sample in a short time(1-2min)The amount of the CO2 inside released;Infrared-gas point Analyzer method, the air-channel system opened by computer installation establish CO2 concentration in system and change the linear relationship with putting into practice to count Calculate the mineralising of organic carbon.These experiment sensing equipments are complicated, and inconvenient, equipment cost is high, if breaking down repair fiber crops It is tired.Organic carbon mineralization is generally required controls temperature in climatic chamber, is limited experimental condition.
Invention content
The present invention provides a kind of indoor soil organic carbon mineralization culture monitoring devices.
The present invention provides following schemes:
A kind of interior soil organic carbon mineralization culture monitoring device, including:
Thermostatic water bath, the thermostatic water bath are used to place the liquid for adjusting cultivation temperature;
Temperature securing component, the temperature securing component are used to obtain the real-time temperature values of the liquid;
Temperature-controlled package, the temperature-controlled package are connected with the temperature securing component, and the temperature-controlled package is used for The fluid temperature, which is adjusted, according to the real-time temperature values got by the temperature securing component remains preset culture temperature Degree;
Culture bottle, the culture bottle are located in the thermostatic water bath, and the culture bottle is for placing culture soil sample to be detected;It is described Culture bottle bottle mouth position is provided with plug in the first rubber;
Absorption bottle, the absorption bottle are located in the thermostatic water bath, the hydrogen in the absorption bottle for placing absorbing carbon dioxide Sodium oxide molybdena liquid;The absorption bottle bottle mouth position is provided with plug in the second rubber;
Conduit, the tube at one end pass through plug in first rubber to extend to inside the culture bottle;The other end passes through described Plug extends to sodium hydroxide liquid levels or less in second rubber.
Preferably:The temperature securing component includes the temperature sensor being located in the thermostatic water bath.
Preferably:The temperature-controlled package includes temperature control box and the heater strip in the thermostatic water bath, described Temperature control box is connected with the temperature sensor and the heater strip respectively.
Preferably:Soil sample culture dish and evaporating dish are placed in the culture bottle, the soil sample culture dish is for placing Culture soil sample to be detected, the evaporating dish is for placing distilled water.
Preferably:It is equipped with draw ring beyond the Great Wall in plug and second rubber in first rubber.
Preferably:The absorption bottle is 50 milliliters of triangular flasks.
Preferably:It is provided with partition board in the thermostatic water bath.
According to specific embodiment provided by the invention, the invention discloses following technique effects:
By the invention it is possible to realize that a kind of indoor soil organic carbon mineralization culture monitoring device is somebody's turn to do in an implementation mode Device may include thermostatic water bath, and the thermostatic water bath is used to place the liquid for adjusting cultivation temperature;Temperature securing component, The temperature securing component is used to obtain the real-time temperature values of the liquid;Temperature-controlled package, the temperature-controlled package with The temperature securing component is connected, and the temperature-controlled package is used for real-time according to being got by the temperature securing component Temperature value adjusts the fluid temperature and remains preset cultivation temperature;Culture bottle, the culture bottle are located at the thermostatic water bath Interior, the culture bottle is for placing culture soil sample to be detected;The culture bottle bottle mouth position is provided with plug in the first rubber;It absorbs Bottle, the absorption bottle are located in the thermostatic water bath, the caustic lye of soda in the absorption bottle for placing absorbing carbon dioxide Body;The absorption bottle bottle mouth position is provided with plug in the second rubber;Conduit, the tube at one end pass through the first rubber Nei Saiyan It extends to inside the culture bottle;The other end passes through plug in second rubber to extend to sodium hydroxide liquid levels or less.This Shen The device that please be provided controls cultivation temperature using the thermostatic water bath of temperature control box control, can provide spy for soil organic carbon mineralization Fixed temperature condition.Culture bottle and absorption bottle separate, and are connected by conduit, with METHOD FOR CONTINUOUS DETERMINATION soil organic carbon mineralization rate and can tire out Product mineralising amount.Culture bottle holds the soil sample incubator of soil and claims have the beaker of certain water to be put into bottle together, in blend rubber Plug sealing, simple in structure, easy to operate, at low cost, the inconvenience, the equipment cost that efficiently solve existing technologies operation be high, structure The problem of the inconvenience such as complexity.A certain amount of lye is held in absorption bottle, triangle bottleneck is equipped with rubber stopper, and rubber stopper is inserted with glass and leads Pipe is connected with culture bottle, can replace triangular flask at any time, to discharging CO2 METHOD FOR CONTINUOUS DETERMINATIONs.
Certainly, it implements any of the products of the present invention and does not necessarily require achieving all the advantages described above at the same time.
Description of the drawings
It in order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will be to institute in embodiment Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the present invention Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings Obtain other attached drawings.
Fig. 1 is a kind of structural representation of indoor soil organic carbon mineralization culture monitoring device provided in an embodiment of the present invention Figure.
In figure:Plug 6, absorption in thermostatic water bath 1, temperature sensor 2, temperature control box 3, heater strip 4, culture bottle 5, the first rubber Plug 8, conduit 9, soil sample culture dish 10, evaporating dish 11, partition board 12 in the 7, second rubber of bottle.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained belong to what the present invention protected Range.
Embodiment
Referring to Fig. 1, for the kind interior soil organic carbon mineralization culture monitoring device that the embodiment of the present invention one provides, such as Fig. 1 Shown, which includes thermostatic water bath 1, and the thermostatic water bath 1 is used to place the liquid for adjusting cultivation temperature;
Temperature securing component, the temperature securing component are used to obtain the real-time temperature values of the liquid;The temperature acquisition group Part includes the temperature sensor 2 being located in the thermostatic water bath.
Temperature-controlled package, the temperature-controlled package are connected with the temperature securing component, the temperature-controlled package Preset training is remained for adjusting the fluid temperature according to the real-time temperature values got by the temperature securing component Support temperature;The temperature-controlled package include temperature control box 3 and in the thermostatic water bath 1 heater strip 4, the temperature control box 4 are connected with the temperature sensor 2 and the heater strip 4 respectively.
Culture bottle 5, the culture bottle 5 are located in the thermostatic water bath 1, and the culture bottle 5 is for placing culture to be detected Soil sample;5 bottle mouth position of the culture bottle is provided with plug 6 in the first rubber;Be placed in the culture bottle 5 soil sample culture dish 10 and Evaporating dish 11, the soil sample culture dish 10 is for placing culture soil sample to be detected, and the evaporating dish 11 is for placing distilled water.
Absorption bottle 7, the absorption bottle 7 are located in the thermostatic water bath 1, absorb dioxy for placing in the absorption bottle 7 Change the sodium hydroxide liquid of carbon;7 bottle mouth position of the absorption bottle is provided with plug 8 in the second rubber;The absorption bottle 7 is 50 milliliter three Angle bottle.
Conduit 9,9 one end of the conduit pass through plug 6 in first rubber to extend to inside the culture bottle 5;The other end Plug 8 extends to sodium hydroxide liquid levels or less in second rubber.
Further, plug 6 and interior fill in of second rubber are equipped with draw ring on 8 in first rubber.The constant temperature Partition board 12 is provided in sink 1.
The device keeps cultivation temperature with thermostatic water bath, is sealed to culture bottle with plug in rubber, often cultivates a timing Between after replace CO2 and absorb triangular flask, titrated with dilute hydrochloric acid solution, while changing CO2 equipped with new lye and absorbing triangle Continue closed culture in bottle.Soil sample incubator weight method adds water to keep constant weight, titrates complete natural ventilation 30min, then Average daily mineralising amount for measuring soil organic matter and Organic carbon decomposition rate.
The device may include temperature control box, temperature sensor, thermostatic water bath, heater strip, culture bottle, gas bottle.Culture bottle Including plug, soil sample culture vessel, evaporating dish, 2L plastic bottles in plastic outer cover, rubber;Gas bottle includes:Three with rubber stopper Angle bottle, the NaOH solution that built-in CO2 absorbs.Draw ring is equipped in the rubber beyond the Great Wall, convenient for handling.Plug is inserted in the rubber Conduit is used for transmission release gas.The CO2 absorption bottles use 50ml triangular flasks, hold lye conveniently, titration is quick.Culture Bottle bottle uses 2L plastic bottles, and bore is larger, is conveniently put into soil sample incubator, and holds the small burning that distilled water keeps air humidity Cup.Small culture dish is placed in culture bottle, big with air contact surfaces, and mineralising is apparent, and it is quick to hold soil sample.
The structure is being using process:
Soil collection:The soil sample sample of acquisition is placed in culture dish, culture dish is put into soil sample culture bottle, soil sample culture bottle Bottle stopper is inserted into grass tube, is connected with gas bottle by conduit, culture bottle is then put into thermostatic water bath;
Temperature controls:Thermostatic water bath temperature is set, temperature regulating device is started, Mineralized Culture is carried out to pedotheque;
CO2 gas collections:The catheter interface that soil sample culture bottle is connected to using gas-guide tube is connected with triangular flask, and the NaOH of triangular flask is molten Liquid absorbs the CO2 discharged during soil organic carbon mineralization.
Mineralization rate measures:The CO2 absorbed with HCl titration measuring NaOH solutions finally converts CO2 Cumulative release amounts For mineralization rate.
The culture assay device measured for soil organic carbon mineralization ability in device provided by the present application has individual control Incubator, thermostatic water bath use iron sheet material, and for additional thermal insulation board convenient for heat preservation, babinet is interior to be equipped with heater strip and temperature sensor, adopts With constant temperature water bath cultivation, setting water temperature is controlled by temperature control box and carries out case body temperature control.It is received equipped with individual gas Collect region, the triangular flask for collecting CO2 can substitute at any time, and realization is continuously cultivated and collected in the same soil sample incubation The CO2 discharged.
In short, device provided by the present application, controls cultivation temperature using the thermostatic water bath of temperature control box control, can be soil Organic carbon mineralization provides specific temperature condition.Culture bottle and absorption bottle separate, and are connected by conduit, can be had with METHOD FOR CONTINUOUS DETERMINATION soil Machine carbon mineralization rate and accumulation mineralising amount.Culture bottle holds the soil sample incubator of soil and claims have the beaker of certain water to be put into together In bottle, plug sealing, simple in structure in blend rubber, easy to operate, at low cost, efficiently solve existing technologies operation inconvenience, Equipment cost is high, the inconvenient problem such as complicated.A certain amount of lye is held in absorption bottle, triangle bottleneck is equipped with rubber stopper, Rubber stopper is inserted with grass tube and is connected with culture bottle, can replace triangular flask at any time, to discharging CO2 METHOD FOR CONTINUOUS DETERMINATIONs.
It should be noted that herein, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those Element, but also include other elements that are not explicitly listed, or further include for this process, method, article or equipment Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that There is also other identical elements in process, method, article or equipment including the element.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the scope of the present invention.It is all Any modification, equivalent replacement, improvement and so within the spirit and principles in the present invention, are all contained in protection scope of the present invention It is interior.

Claims (7)

1. a kind of interior soil organic carbon mineralization culture monitoring device, which is characterized in that including:Thermostatic water bath, the thermostatted water Slot is used to place the liquid for adjusting cultivation temperature;Temperature securing component, the temperature securing component is for obtaining the liquid The real-time temperature values of body;Temperature-controlled package, the temperature-controlled package are connected with the temperature securing component, the temperature tune Section component is used to be remained according to the real-time temperature values adjusting fluid temperature got by the temperature securing component pre- The cultivation temperature set;Culture bottle, the culture bottle are located in the thermostatic water bath, and the culture bottle is for placing culture to be detected Soil sample;The culture bottle bottle mouth position is provided with plug in the first rubber;Absorption bottle, the absorption bottle are located in the thermostatic water bath, Sodium hydroxide liquid in the absorption bottle for placing absorbing carbon dioxide;The absorption bottle bottle mouth position is provided with the second rubber Interior plug;Conduit, the tube at one end pass through plug in first rubber to extend to inside the culture bottle;The other end passes through described Plug extends to sodium hydroxide liquid levels or less in second rubber.
2. interior soil organic carbon mineralization culture monitoring device according to claim 1, which is characterized in that the temperature obtains It includes the temperature sensor being located in the thermostatic water bath to take component.
3. interior soil organic carbon mineralization culture monitoring device according to claim 2, which is characterized in that the temperature tune Section component includes temperature control box and the heater strip in the thermostatic water bath, the temperature control box respectively with the temperature sensor And the heater strip is connected.
4. interior soil organic carbon mineralization culture monitoring device according to claim 1, which is characterized in that the culture bottle It is inside placed with soil sample culture dish and evaporating dish, the soil sample culture dish is for placing culture soil sample to be detected, the evaporating dish For placing distilled water.
5. interior soil organic carbon mineralization culture monitoring device according to claim 1, which is characterized in that first rubber It is equipped with draw ring beyond the Great Wall in plug and second rubber in glue.
6. interior soil organic carbon mineralization culture monitoring device according to claim 1, which is characterized in that the absorption bottle For 50 milliliters of triangular flasks.
7. interior soil organic carbon mineralization culture monitoring device according to claim 1, which is characterized in that the thermostatted water Partition board is provided in slot.
CN201810473621.XA 2018-05-17 2018-05-17 A kind of interior soil organic carbon mineralization culture monitoring device Pending CN108362825A (en)

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111077286A (en) * 2019-12-31 2020-04-28 重庆三峡学院 Method for evaluating influence of dry-wet cycle regulation on decomposition of active and inert carbon reservoirs
CN111812089A (en) * 2020-07-24 2020-10-23 北京林业大学 Mineralization of CO by organic carbon in soil2Device and method for continuously measuring release amount

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201975470U (en) * 2011-03-15 2011-09-14 肇庆理士电源技术有限公司 Constant-temperature water groove
CN205109686U (en) * 2015-10-31 2016-03-30 浙江沪江线业有限公司 Water bath with thermostatic control oscillator
CN206096125U (en) * 2016-10-26 2017-04-12 贵州大学 Device of organic carbon mineralize mineralization of indoor survey soil
CN106980007A (en) * 2017-03-09 2017-07-25 河海大学 The culture apparatus and assay method determined for soil organic carbon mineralization ability
CN206762921U (en) * 2017-05-23 2017-12-19 燕山大学 A kind of multichannel circulation constant temperature water bath device
CN206945389U (en) * 2017-06-29 2018-01-30 中国农业科学院草原研究所 A kind of soil organic carbon mineralization culture apparatus
CN208239382U (en) * 2018-05-17 2018-12-14 中国农业科学院农田灌溉研究所 A kind of interior soil organic carbon mineralization culture monitoring device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201975470U (en) * 2011-03-15 2011-09-14 肇庆理士电源技术有限公司 Constant-temperature water groove
CN205109686U (en) * 2015-10-31 2016-03-30 浙江沪江线业有限公司 Water bath with thermostatic control oscillator
CN206096125U (en) * 2016-10-26 2017-04-12 贵州大学 Device of organic carbon mineralize mineralization of indoor survey soil
CN106980007A (en) * 2017-03-09 2017-07-25 河海大学 The culture apparatus and assay method determined for soil organic carbon mineralization ability
CN206762921U (en) * 2017-05-23 2017-12-19 燕山大学 A kind of multichannel circulation constant temperature water bath device
CN206945389U (en) * 2017-06-29 2018-01-30 中国农业科学院草原研究所 A kind of soil organic carbon mineralization culture apparatus
CN208239382U (en) * 2018-05-17 2018-12-14 中国农业科学院农田灌溉研究所 A kind of interior soil organic carbon mineralization culture monitoring device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111077286A (en) * 2019-12-31 2020-04-28 重庆三峡学院 Method for evaluating influence of dry-wet cycle regulation on decomposition of active and inert carbon reservoirs
CN111812089A (en) * 2020-07-24 2020-10-23 北京林业大学 Mineralization of CO by organic carbon in soil2Device and method for continuously measuring release amount

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