CN206132546U - Detect quick biodegradability's of chemicals device - Google Patents
Detect quick biodegradability's of chemicals device Download PDFInfo
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- CN206132546U CN206132546U CN201621110490.1U CN201621110490U CN206132546U CN 206132546 U CN206132546 U CN 206132546U CN 201621110490 U CN201621110490 U CN 201621110490U CN 206132546 U CN206132546 U CN 206132546U
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- bottle
- carbon dioxide
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- bottles
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Abstract
The utility model relates to a detect the quick biodegradable's of chemicals device ( carbon dioxide produces the assay method), it has included the box that has temperature control mechanism and venting mechanism, temperature control mechanism comprises 2 water baths, venting mechanism includes 6 aeration pump, and oils and carbon dioxide are got rid of through water and alkali lye to air that every aeration pump produced, ventilate to the culture solution in the blake bottle, and the carbon dioxide who produces in the culture solution is through the detected bottle of afterbody absorption carbon dioxide. The utility model discloses device simple accurate, maneuverability is strong. Equipment structure is simple, the connecting line is simple, make and maintain conveniently, can be used to that dissolve the aquatic, the indissoluble is separated, the quick biodegradable of type chemicals such as semi -volatile and adsorptivity is experimental.
Description
Technical field
The utility model is related to a kind of device of detection chemicals biological degradability, and in particular to one kind is by calculating CO2
Yield is characterizing the experimental rig of organic chemicals rapid biodegradability.
Background technology
Rapid biodegradability refers to that tested material contacts the biodegradability for showing in limiting time with inoculum,
It is to carry out the current Organization of Economy and Cooperation Development of the important indicator of harmfulness discriminating and risk assessment (OECD) to chemicals to push away
The classical method of testing for going out includes dissolved organic carbon (DOC) abatement test (301A), carbon dioxide (CO2) produce test
(301B), improved MITI test (I) (301C), improved OECD screening tests (301E) and exhale by closed bottle test (301D)
Inhale measurement Law test (301F).Wherein 301A, 301D and 301E test program relative ease, DOC indexs have special TOC analyses
Instrument measuring；For 301C and 301F, there is more ripe oxygen respirometer and other corollary equipments both at home and abroad, because
This test job more can smoothly be carried out, and 301B be had only, due to CO2Yield and system is bubble-tight is difficult to control to, to mesh
Before till, people cannot using it is a kind of effectively and practical test device by determining 28d carbon dioxide yield determining drop
Solution rate.And due to the particularity of specialty test, also seldom there is the complete instrument and equipment for this kind of test in the market.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of for chemicals rapid biodegradability test (two
Carbonoxide produce determination method) detection means, so as to the fast degraded biologically of more preferable evaluating chemical product.
To solve above-mentioned technical problem, the technical solution of the utility model is as follows：
A kind of device of detection chemicals fast degraded biologically, it includes ventilation unit, culture unit and detector unit, institute
State three units to be integrated in a casing；The ventilation unit includes that 1 aeration pump, 1 gas flowmeter, 1 air are net
Gas cylinder and 2 carbon dioxide absorption bottles, the culture unit includes 1 blake bottle and 1 water-bath, and the detector unit includes
3 detection bottles, the aeration pump and gas flowmeter are connected with air inlet bottle entrance, and air inlet bottle is exported and 2 dioxies
Change carbon absorption bottle to be sequentially connected, the gas vent of second carbon dioxide absorption bottle is connected with the blake bottle in culture unit, trains
Foster bottle is placed in water-bath, carries out temperature control；The outlet of the blake bottle is sequentially connected with 3 detection bottles in detector unit.
Specifically, described device includes 6 ventilation units, 6 culture units and 6 detector units, and 6 covering devices are mutual
It is independent.Respectively to each culture unit Input gas, and flow control can be carried out, Jing water and alkali lye remove oils and carbon dioxide
Air, the nutrient solution in blake bottle is ventilated, absorb the carbon dioxide of the biodegradable middle generation of tested material.
Specifically, pure water is housed in described air air cleaning bottle, alkali lye is housed in the carbon dioxide absorption bottle.
Specifically, barium hydroxide, the CO that tested material cultivation stage gives off are housed in the detection bottle2The detection of Jing afterbodys
Bottle absorbing carbon dioxide, then quantitative determined.
Beneficial effect：
The utility model device is simple and easy to do, workable, can simultaneously carry out multigroup experiment, effectively improves work effect
Rate.
This device structure is simple, connecting line is simple, manufacture and easy to maintenance.
This equipment can be used for the fast degraded biologically of the class chemicals such as dissolving in water, indissoluble solution, half volatile and adsorptivity
Test.
Description of the drawings
Fig. 1：A kind of device front view of detection chemicals fast degraded biologically that the utility model is related to；
Fig. 2：A kind of device top view of detection chemicals fast degraded biologically that the utility model is related to；
Fig. 3：A kind of device left view of detection chemicals fast degraded biologically that the utility model is related to；
Fig. 4：A kind of device pneumatic diagram of detection chemicals fast degraded biologically that the utility model is related to；
Fig. 5：Blake bottle, CO2 absorb in a kind of device of detection chemicals fast degraded biologically that the utility model is related to
Bottle and detection bottle connection figure.
Wherein：1. power switch；2. timing knob；3. pumping source of ventilating is switched；4. mass air flow sensor；5. air valve；6. ball
Valve；7. gas path pipe；8. rubber hose；9. timed unit；10. socket；11. ventilation pumps；12.250ml CO2Absorption bottle；
13.250ml detection bottle；14.2.5L blake bottle；
Specific embodiment
Technical solutions of the utility model are further described below by way of example.
It, to explanation of the present utility model, is not to restriction of the present utility model that this specific embodiment is only.This area
Technical staff easily read and the present embodiment can be made after specification the modification without creative contribution, but as long as at this
All protected by Patent Law in the right of utility model.
Embodiment 1：A kind of device of detection chemicals fast degraded biologically
A kind of device of detection chemicals fast degraded biologically, it includes ventilation unit, culture unit and detector unit, institute
State three units to be integrated in a casing；The ventilation unit includes that 1 aeration pump, 1 gas flowmeter, 1 air are net
Gas cylinder and 2 carbon dioxide absorption bottles, the culture unit includes 1 blake bottle and 1 water-bath, and the detector unit includes
3 detection bottles, the aeration pump and gas flowmeter are connected with air inlet bottle entrance, and air inlet bottle is exported and 2 dioxies
Change carbon absorption bottle to be sequentially connected, the gas vent of second carbon dioxide absorption bottle is connected with the blake bottle in culture unit, trains
Foster bottle is placed in water-bath, carries out temperature control；The outlet of the blake bottle is sequentially connected with 3 detection bottles in detector unit.
Described device includes 6 ventilation units, 6 culture units and 6 detector units, and 6 covering devices are separate.Can
Respectively to each culture unit Input gas, and flow control is carried out, the air of Jing water and alkali lye removal oils and carbon dioxide,
Nutrient solution in blake bottle is ventilated, the carbon dioxide of the biodegradable middle generation of tested material is absorbed.
Pure water is housed in described air air cleaning bottle, alkali lye is housed in the carbon dioxide absorption bottle.
Barium hydroxide, the CO that tested material cultivation stage gives off are housed in the detection bottle2The detection bottle of Jing afterbodys absorbs
Carbon dioxide, then quantitative determined.
Embodiment 2：Test procedure and data processing
Activated sludge is gathered to laboratory from Nanjing sewage treatment plants, deionized water fully wash after again
Be configured to concentration be 4g (dry weight)/L suspension as inoculum.
200mL pure water is equipped with 22 ± 2 DEG C of 5 environment, air by 1, two are equipped with 200mL 0.5mol/L hydrogen
The CO of sodium hydroxide solution2Absorption bottle, with the flow velocity of 30～100mL/min air is passed through, to remove oils and carbon dioxide.Afterbody
3 detection bottles of the barium hydroxide equipped with 0.0125mol/L of connection.Add test molten in the test blake bottle of the brown of 2.5L
Liquid, tested material concentration is set in 10～20mg TOC/L.Before on-test, in 6 blake bottles inorganic medium is separately added into
And inoculum, it is passed through the air 24h of carbon dioxide removal.After ventilation, the tested material of normal concentration, each culture are added in test group
Bottle batch arranges as follows：Comprising tested material, inoculum and culture medium by trial jar；It is only blank right containing inoculum and culture medium
According to and containing reference substance, inoculum and culture medium program control.
After on-test, each barium hydroxide reaction for processing the carbon dioxide for producing and detecting in bottle, the product of carbon dioxide
Raw amount is determined by titrating remaining barium hydroxide with 0.05mol/L HCl.Determining CO2During yield, tear open from device
Going out to be directly connected to the barium hydroxide detection bottle of blake bottle is used to titrate, and other two detection bottle order reach is connected to test bottle,
And a new barium hydroxide prepared detects bottle in the connection of end, test period is 28d.Dioxy is determined by potentiometric titrimeter
Change carbon yield.
Carbon dioxide yield is determined by remaining alkali lye amount in detection bottle, when the hydroxide using 0.0125mol/L
When barium solution makees absorbing liquid, carbon dioxide yield is determined by the HCI of 0.05mol/L.(therefore 50mL hydrochloric acid drips
Determine 100mL barium hydroxides).
Because the mol ratio that the carbon dioxide for producing reacts with barium hydroxide is 1：1, the reaction mole of hydrochloric acid and barium hydroxide
Than being 2：1, form barium chloride with remaining barium hydroxide in HCI absorption bottle, it is known that the molal weight of carbon dioxide is
44g, carbon dioxide yield is calculated by formula 1：
Biodegradation percentage is calculated by formula 2：
Method is required
(1) the inorganic carbon content of culture medium is less than 5%, during off-test, CO in blank detection bottle2Yield be less than
40mg/L。
(2) biological degradability of reference substance 14d reaches 60%.
(3) difference of the biological degradation rate parallel test result of tested material is less than 20%
Embodiment 2：Aniline test
As shown in Figure 1, the device of detection chemicals fast degraded biologically of the present utility model, an including casing, internal 6
Individual unit, can simultaneously carry out the test of 6 groups of samples.1st, power switch；2nd, timing knob；3rd, pumping source of ventilating is switched；4th, air
Flowmeter；5th, air valve；6th, ball valve.Each unit and an outside tissue culture are supported unit and be connecteds, in placement temperature regulating device, temperature regulating device by
Water-bath is constituted.
As shown in figure 5, in 3 250ml CO of long tube connection2Load scavenging solution in absorption bottle, be to remove in insufflation gas
Carbon dioxide and oily substance, it is ensured that in incubation without excessive carbon dioxide enter, tested material be unique organic carbon source,
Experimental test data are not affected.Load the detection liquid (barium hydroxide of 0.0125mol/L in 3 250ml detection bottles of short tube connection
Solution), it is the carbon dioxide for absorbing tested material release in blake bottle.Under dark condition, in three groups of 2.5L blake bottles, it is separately added into
Inorganic medium and inoculum, add aniline, make concentration of aniline be 15.5mg TOC/L；Three groups of blake bottles of blank control test
In only add inorganic medium and inoculum.
As shown in figure 3, opening air valve, the air for making removing carbon dioxide is passed through in blake bottle, is cultivated.Setting time
28 days.Barium carbonate sediment content in quantitative test reaction bulb, determines carbon dioxide production amount, to determine degrading aniline rate.
The HCI result of table 1
The biological degradation rate result of table 2
During off-test, CO in blank2Accumulation yield be less than 40mg CO2/ L, aniline (initial concentration
15.5mg TOC/L) 28d biological degradation rates be 84.93% (>60%), aniline determines relative standard deviation for three times and is less than
1.83%, result of the test is effective.
Embodiment 3：Sodium benzoate test
As shown in figure 5, in 3 250ml CO of long tube connection2Load scavenging solution in absorption bottle, be to remove in insufflation gas
Carbon dioxide and oily substance, it is ensured that in incubation without excessive carbon dioxide enter, tested material be unique organic carbon source,
Experimental test data are not affected.Load the detection liquid (hydroxide of 0.0125mol/L in 3 250ml detection bottles of short tube connection
Barium solution), it is the carbon dioxide for absorbing tested material release in blake bottle.Under dark condition, by Sodium Benzoate 15.5mg TOC/L
It is another to add culture medium and domestic sewage treatment plant without the activated sludge of domestication as connecing in being respectively put into three groups of 2.5L blake bottles
Kind thing, totally three groups；Culture medium and inoculum are only added in three groups of blake bottles of blank control test.
As shown in figure 5, opening air valve, the air for making removing carbon dioxide is passed through in blake bottle, is cultivated.Setting time
28 days.Barium carbonate sediment content in quantitative test blake bottle, to determine degradation rate.
The HCI result of table 3
The biological degradation rate result of table 4
During off-test, CO in blank2Accumulation yield be less than 40mg CO2/ L, Sodium Benzoate (initial concentration
15.5mg TOC/L) 28d biological degradation rates be 87.78% (>60%), Sodium Benzoate determines relative standard deviation for three times and is less than
2.85%, result of the test is effective.
Claims (4)
1. a kind of device of detection chemicals fast degraded biologically, it is characterised in that it includes ventilation unit, culture unit and inspection
Unit is surveyed, three units are integrated in a casing；The ventilation unit include 1 aeration pump, 1 gas flowmeter, 1
Individual air air cleaning bottle and 2 carbon dioxide absorption bottles, the culture unit includes 1 blake bottle and 1 water-bath, the detection
Unit includes 3 detection bottles, and the aeration pump and gas flowmeter are connected with air inlet bottle entrance, air inlet bottle export and
2 carbon dioxide absorption bottles are sequentially connected, the blake bottle in the gas vent and culture unit of second carbon dioxide absorption bottle
It is connected, blake bottle is placed in water-bath, outlet and 3 detection bottles in detector unit of the blake bottle are sequentially connected.
2. it is according to claim 1 it is a kind of detection chemicals fast degraded biologically device, it is characterised in that described dress
Put including 6 ventilation units, 6 culture units and 6 detector units, 6 covering devices are separate.
3. it is according to claim 1 it is a kind of detection chemicals fast degraded biologically device, it is characterised in that described sky
Pure water is housed in gas air cleaning bottle, alkali lye is housed in the carbon dioxide absorption bottle.
4. it is according to claim 1 it is a kind of detection chemicals fast degraded biologically device, it is characterised in that the detection
Barium hydroxide is housed in bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621110490.1U CN206132546U (en) | 2016-10-10 | 2016-10-10 | Detect quick biodegradability's of chemicals device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621110490.1U CN206132546U (en) | 2016-10-10 | 2016-10-10 | Detect quick biodegradability's of chemicals device |
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| Publication Number | Publication Date |
|---|---|
| CN206132546U true CN206132546U (en) | 2017-04-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621110490.1U Expired - Fee Related CN206132546U (en) | 2016-10-10 | 2016-10-10 | Detect quick biodegradability's of chemicals device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114428168A (en) * | 2022-01-26 | 2022-05-03 | 北京托摩根生物科技有限公司 | Biodegradation instrument for composting method |
| CN114487371A (en) * | 2022-01-26 | 2022-05-13 | 北京托摩根生物科技有限公司 | Water planting method biodegradation instrument |
-
2016
- 2016-10-10 CN CN201621110490.1U patent/CN206132546U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114428168A (en) * | 2022-01-26 | 2022-05-03 | 北京托摩根生物科技有限公司 | Biodegradation instrument for composting method |
| CN114487371A (en) * | 2022-01-26 | 2022-05-13 | 北京托摩根生物科技有限公司 | Water planting method biodegradation instrument |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170426 Termination date: 20211010 |