CN110184187A - The online scope of minicell culture - Google Patents
The online scope of minicell culture Download PDFInfo
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- CN110184187A CN110184187A CN201910608500.6A CN201910608500A CN110184187A CN 110184187 A CN110184187 A CN 110184187A CN 201910608500 A CN201910608500 A CN 201910608500A CN 110184187 A CN110184187 A CN 110184187A
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- workbench
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- 230000005540 biological transmission Effects 0.000 claims abstract description 11
- 238000005286 illumination Methods 0.000 claims abstract description 11
- 239000012530 fluid Substances 0.000 claims abstract description 10
- 238000003860 storage Methods 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 12
- 230000003321 amplification Effects 0.000 abstract description 6
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 6
- 238000011065 in-situ storage Methods 0.000 abstract description 4
- 230000001413 cellular effect Effects 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000000799 fluorescence microscopy Methods 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- HGAZMNJKRQFZKS-UHFFFAOYSA-N chloroethene;ethenyl acetate Chemical compound ClC=C.CC(=O)OC=C HGAZMNJKRQFZKS-UHFFFAOYSA-N 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of online scopes of minicell culture, include workbench, micro- behaviour's column is provided on workbench, transverse arm is provided on micro- behaviour's column, illumination apparatus is provided on transverse arm, culture dish is provided on the table top of workbench, sample is placed in culture dish, the lower section of workbench is closed case, minitype inversion microscope is provided in closed case, there is auto-focusing adjusting bracket below minitype inversion microscope, culture dish is connected by catheter with fluid storage and transmitter, there is wireless transmission antenna on inverted fluorescence microscope.The back of workbench has the antenna of wireless transmission, for being wirelessly transferred the experimental data of record.Product of the present invention have the characteristics that it is small in size, can be put into inside routine culture case in real time, in situ detection cellular morphology pattern, acquired results can be real-time transmitted on computer or mobile phone, even if experimenter is facilitated to observe；The amplification factor of object lens can also be stimulated cell, can be automatically adjusted by adjusting the intensity, wave-length coverage or the other liquid of filling of different light sources, guarantee that tested sample is imaged under different amplification.
Description
Technical field
The present invention relates to a kind of online scopes of minicell culture, belong to technical field of biological.
Background technique
Cell culture needs routine observation, such as growthform, has pollution-free, pH value, cell count, survival test letter
Breath.Cell observation system is larger at present, mainly fluorescence microscope, and volume is larger, and cell is after incubator culture for a period of time
Need often to take out to be put on microscope and observe, be easy contamination of cells, it is time-consuming it is bothersome, can not in real time, in-situ monitoring cell
State.
Summary of the invention
Technical problem to be solved by the invention is to provide it is a kind of can observe in real time, safe and reliable, cell easy to use
Incubator.
The technical scheme is that
A kind of online scope of minicell culture includes workbench, is provided with micro- behaviour's column on workbench, sets on micro- behaviour's column
It is equipped with transverse arm, illumination apparatus is provided on transverse arm, culture dish is provided on the table top of workbench, places sample, work in culture dish
The lower section for making platform is closed case, and minitype inversion microscope is provided in closed case, is had below minitype inversion microscope automatic
Focal adjustment frame, culture dish are connected by catheter with fluid storage and transmitter, there is wireless transmission on inverted fluorescence microscope
Antenna.
The beneficial effects of the present invention are:
The present invention is built-in in conventional carbon dioxide incubator, since volume is very small (162*132*180mm), the scope
It can be placed directly in conventional carbon dioxide incubator, liquid-adding device can also be placed into incubator simultaneously as needed, be passed through
The outer remote control operation of case fills other liquid in Tissue Culture Dish.
It is able to achieve real-time monitoring, since microscope is constantly in working condition, the virtual condition of cell during the cultivation process,
It with actual observation and can record；It can be wirelessly transferred as a result, result is recorded via wireless transport module and wireless transmission
In antenna transmission to the computer or mobile phone of outside, experimenter can check the growth conditions of cell at any time；Changeable cell is raw
Long environment: the growing environment of cell can be changed in wavelength, intensity and additional liquid by adjusting illumination light source；It is automatic right
Burnt: since cell size has differences, different cells need to focus again, and being inverted mini microscope can be carried out automatically by software
Focus adjustment；Fluorescence imaging: being inverted mini microscope has the function of light field imaging and fluorescence imaging.
Product of the present invention have the characteristics that it is small in size, can be put into inside routine culture case in real time, in situ detection cellular morphology shape
Looks, acquired results can be real-time transmitted on computer or mobile phone, facilitate experimenter's immediate observation；It can be by adjusting the strong of different light sources
Degree, wave-length coverage and increase other interference liquid to stimulate cell, can also automatically adjust the amplification factor of object lens, guarantee
Tested sample is imaged under different amplification.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention.
Fig. 2 has cultivated the fluorogram of 1 hour cell before being liquid feeding；
Fig. 3 is the fluorogram that light intensity increases to 1 hour after 50mW；
Fig. 4 is that the solution 100uL that pH is 7.5, the cell shape after 1 hour is added by liquid feeding transmitter 9 after observation 2 hours
Looks change figure；
Fig. 5 is to check cytological map be recorded after Fig. 4 is observed 4 hours.
In figure: 1- illumination apparatus；The micro- behaviour's column of 2-；3- sample；4- culture dish；5- wireless transmission antenna；6- minitype inversion
Microscope；7- auto-focusing adjusting bracket；8- catheter；9- fluid storage and transmitter；10- workbench；11- transverse arm.
Specific embodiment
Below with reference to specific drawings and examples, the invention will be further described.
A kind of online scope of minicell culture includes a workbench 10, is provided with micro- behaviour's column on workbench 10
2, transverse arm 11 is provided on micro- behaviour's column 2, and illumination apparatus 1 is provided on transverse arm 11, culture is provided on the table top of workbench 10
Ware 4, culture dish 4 is interior to place sample 3, and the lower section of workbench 10 is closed case, and it is micro- to be provided with minitype inversion in closed case
Mirror 6, there is auto-focusing adjusting bracket 7 below minitype inversion microscope 6, and culture dish 4 passes through catheter 8 and fluid storage and transmitter
9 are connected, and have wireless transmission antenna 5 on inverted fluorescence microscope 6.The table top of workbench 10 is transparent glass.
Micro- about 2 behaviour's column moves left and right illumination apparatus 1, and after sample cell is placed into culture dish, entire scope can be straight
It connects to be placed in inside conventional carbon dioxide incubator and carries out online cell culture and observation, the image or screen observed can be by nothings
Line transmitting device 5 is transferred on external computer or mobile phone；Liquid is interfered to be injected into training by fluid storage and transmitter 9 and catheter
It supports inside ware.
Illumination apparatus 1 has different wave length, intensity control.10 dimension volume of cabinet of the present invention is small (162*132*180mm),
Sample can be put into real-time, in situ detection cellular morphology pattern, acquired results inside routine culture case can be real-time transmitted to computer
Or on mobile phone, facilitate experimenter's immediate observation；Cell can also be carried out by adjusting intensity, the wave-length coverage of different light sources
Stimulation, the amplification factor that can automatically adjust object lens guarantee that tested sample is imaged under different amplification.
Auto-focusing imaging can be carried out by auto-focusing frame 7 between inverted fluorescence microscope 6 and sample 3；Imaging results (depending on
Frequency or picture) it can be transmitted on PC or mobile phone via radio transmission antenna 5；It 1 position of illumination apparatus can be by micro- behaviour's column 2
Carry out upper-lower, left-right positions adjusting；The wavelength of illumination apparatus 1 can customize selection according to demand；Additional liquid is via fluid storage
And transmitter 9 and catheter 8 enter in culture dish 4, speed and volume are controllable；Focusing frame, micro- behaviour's column and radio transmission antenna
It is each attached to above workbench 10, workbench is in sealing state, prevents gas or liquid in incubator from entering influence micro-
Mirror function.
Illumination apparatus 1 of the invention can produce for U.S. ThorLabs company, model: M365D1, wavelength: 365nm；Or type
Number: M488D1, wavelength: 488nm；Or model: M530D1, wavelength: 530nm also can choose other wavelength；Micro- behaviour's column 2 is
The auspicious open-birth produce in Jiangsu, model: GDG300；Wireless transmission antenna 5 is that Shenzhen Shi Jing wireless technology company produces, model:
6689；Minitype inversion microscope 6 is the auspicious open-birth produce in Jiangsu, model: MMSP1000；Auto-focusing adjusting bracket 7 is that Jiangsu is auspicious bright
Biological production, model: MMSS1000；Catheter 8 is Puri Changzhou Puri fluid technique, model: Tygon R-3605；Fluid storage
And transmitter 9 is Puri Changzhou Puri fluid technique, model: PO300；Workbench 10 be the auspicious open-birth produce in Jiangsu, model:
GZT300).
Embodiment
Experimental material: sample is HT55 (human colon cancer cell, Cobioer company production, article No. CBP60012)；It is miniature
Inverted fluorescence microscope: have 10*, 20*, 40* object lens, can fluorescence imaging, can by auto-focusing frame carry out focal length be automatically aligned to,
Minimum resolution 1um；Micro- behaviour's column: can X-axis move horizontally, Z axis vertically move lighting source, minimum precision 100um, maximum row
Journey 150mm；Lighting source: wavelength: 365nm；Catheter 8: internal diameter 500um.
Experimentation:
Sample cell is uniformly mixed in phosphate buffered saline solution-PBS, is placed in culture dish；Cell cultivates instrument placement online
In in carbon dioxide incubator, 24 degree of temperature, 22 degree of temperature；Start lighting source, optical power 10mW, light source is about from cell
100mm distance；Start miniature fluorescence microscope and focuses on computers, start recording pattern；(such as Fig. 2 after observation 1 hour
It is shown), light intensity is turned up to 50mW, observation 1 hour is continued；Cell morphology changes (as shown in Figure 3)；After observation 2 hours,
The solution 100uL that pH is 7.5 is added by liquid feeding transmitter 9, cell morphology changes (as shown in Figure 4) after 1 hour；
Observation checked cell picture (as shown in Figure 5) be recorded after 4 hours respectively.
Experimental result:
Detect the fluorescence picture that 1 hour cell has been cultivated before liquid feeding, shown in Fig. 2, light intensity increases to after 50mW 1 hour
Fluorescence picture, shown in Fig. 3, the picture of individual cells is the cell of red circle label in Fig. 2 in picture, as can be seen from Figure carefully
Born of the same parents obviously become larger, it was demonstrated that light intensity is to the irritating effect of cell；1 hour cell name field picture, filling pH are molten after filling pH solution
After liquid 4 hours, there is volume-diminished phenomenon in cell, it was demonstrated that the solution influenced growth of cell of the pH of filling, or even have poisoning
It influences.Shade in Fig. 4 and Fig. 5 is the imaging of liquid-feeding tube.
Claims (1)
1. a kind of online scope of minicell culture includes workbench (10), it is characterised in that: set on the workbench (10)
It is equipped with micro- behaviour's column (2), transverse arm is provided on micro- behaviour's column, is provided on transverse arm illumination apparatus (1), the table top of workbench (10)
On be provided with culture dish (4), place sample (3) in culture dish (4), the lower section of workbench (10) is closed case, closed case
It is inside provided with minitype inversion microscope (6), has auto-focusing adjusting bracket (7) below minitype inversion microscope (6), culture dish (4)
It is connected by catheter (8) with fluid storage and transmitter (9), there are wireless transmission antenna (5) on inverted fluorescence microscope (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910608500.6A CN110184187A (en) | 2019-07-08 | 2019-07-08 | The online scope of minicell culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910608500.6A CN110184187A (en) | 2019-07-08 | 2019-07-08 | The online scope of minicell culture |
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| Publication Number | Publication Date |
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| CN110184187A true CN110184187A (en) | 2019-08-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910608500.6A Pending CN110184187A (en) | 2019-07-08 | 2019-07-08 | The online scope of minicell culture |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110741852A (en) * | 2019-12-03 | 2020-02-04 | 江苏瑞明生物科技有限公司 | Full-automatic plant breeding monitoring system |
| CN111321078A (en) * | 2020-03-05 | 2020-06-23 | 吉林大学 | Cell culture box, control method thereof and culture dish |
| CN118440813A (en) * | 2024-05-06 | 2024-08-06 | 重庆联庆瑞奇科技有限公司 | Vapor condensation prevention device and living cell imager |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201583500U (en) * | 2009-12-29 | 2010-09-15 | 华南理工大学 | Cell real-time observing device in culturing box |
| CN102289066A (en) * | 2011-08-12 | 2011-12-21 | 北京航空航天大学 | Automatic microscopic imaging system for multicellutar culture course |
| CN102827758A (en) * | 2012-09-24 | 2012-12-19 | 长春理工大学 | Cell microscopic observation monitor in cell factory |
| CN105136665A (en) * | 2015-08-17 | 2015-12-09 | 宁波键一电子信息技术有限公司 | Network-based intelligent imaging analyzer for living cell culture in box |
| CN205241704U (en) * | 2015-08-24 | 2016-05-18 | 杭州金源生物技术有限公司 | Intelligent long -range observation monitoring devices of cell culture case |
| CN106010953A (en) * | 2016-06-30 | 2016-10-12 | 长春理工大学 | In-situ real-time microscopic monitoring device and method of cell growth state in cell fermentation tank |
| CN206872853U (en) * | 2017-06-17 | 2018-01-12 | 王江涵 | Biological cell culture apparatus |
-
2019
- 2019-07-08 CN CN201910608500.6A patent/CN110184187A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201583500U (en) * | 2009-12-29 | 2010-09-15 | 华南理工大学 | Cell real-time observing device in culturing box |
| CN102289066A (en) * | 2011-08-12 | 2011-12-21 | 北京航空航天大学 | Automatic microscopic imaging system for multicellutar culture course |
| CN102827758A (en) * | 2012-09-24 | 2012-12-19 | 长春理工大学 | Cell microscopic observation monitor in cell factory |
| CN105136665A (en) * | 2015-08-17 | 2015-12-09 | 宁波键一电子信息技术有限公司 | Network-based intelligent imaging analyzer for living cell culture in box |
| CN205241704U (en) * | 2015-08-24 | 2016-05-18 | 杭州金源生物技术有限公司 | Intelligent long -range observation monitoring devices of cell culture case |
| CN106010953A (en) * | 2016-06-30 | 2016-10-12 | 长春理工大学 | In-situ real-time microscopic monitoring device and method of cell growth state in cell fermentation tank |
| CN206872853U (en) * | 2017-06-17 | 2018-01-12 | 王江涵 | Biological cell culture apparatus |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110741852A (en) * | 2019-12-03 | 2020-02-04 | 江苏瑞明生物科技有限公司 | Full-automatic plant breeding monitoring system |
| CN111321078A (en) * | 2020-03-05 | 2020-06-23 | 吉林大学 | Cell culture box, control method thereof and culture dish |
| CN111321078B (en) * | 2020-03-05 | 2023-12-22 | 吉林大学 | Cell culture box, control method thereof and culture dish |
| CN118440813A (en) * | 2024-05-06 | 2024-08-06 | 重庆联庆瑞奇科技有限公司 | Vapor condensation prevention device and living cell imager |
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Application publication date: 20190830 |