CN105136665B - Living cells culture network-type intelligence imaging analysis instrument in case - Google Patents
Living cells culture network-type intelligence imaging analysis instrument in case Download PDFInfo
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- CN105136665B CN105136665B CN201510535321.6A CN201510535321A CN105136665B CN 105136665 B CN105136665 B CN 105136665B CN 201510535321 A CN201510535321 A CN 201510535321A CN 105136665 B CN105136665 B CN 105136665B
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Abstract
The invention discloses living cells culture network-type intelligence imaging analysis instrument in a plants case, it is set directly at during use in cell incubator, intelligence degree is high, it includes workbench, it is arranged on the stent of workbench side, by the micro- light source mechanism for being arranged on bench-top side, L-type microoptic mechanism, the microscopic imaging device that photoelectronic imaging parts are formed, the image of device intake is digitized into converting transmission to high in the clouds, it is connected by network with computer or mobile phone, it further includes the objective table for culture dish holding, carrier precision rotation platform mechanism, transverse moving mechanism and longitudinal moving mechanism, micro- light source mechanism is arranged on cantilever tip and directly over objective table.Its advantage is：The non-contact observational study of cell culture can be achieved, i.e., need not take out culture dish and view and emulate under the microscope, clearly observation incubator inner cell culture real-time change situation can be realized on computer or mobile phone.
Description
Technical field
The present invention relates to a kind of technical field of cell culture, is especially a kind of case that can be used on cell incubator
Interior living cells culture network-type intelligence imaging analysis instrument.
Background technology
In recent years, the development of Bluetooth technology is getting faster, also more and more extensive in each field application, especially in computer and
The development of application on mobile phone is particularly rapid, and the application range of Bluetooth technology runs far deeper than these.Present more and more people
Studying and moving towards Bluetooth technology among our daily lifes, and having certain effect.Pass through bluetooth and WiFi technology
Combination, with reference to cell phone application so that equipment is more intelligent, and not only data can be synchronized to cloud server, record user's
Daily data message, by cloud computing, can also analyze various reports to user by daily data message.
To the environmental requirement in incubator and its stringent, current tradition in cell cultivation process in bioengineering field
Cell incubator although control system is suppressed using micro computer automatically in terms of environment in control cabinet, can effectively avoid and cultivated
Chamber door is opened in journey causes inner cavity temperature fluctuation big, but to take out culture dish every time for researcher and be observed
It is still a complicated job.Non-contact view and emulate need not take out culture dish under the microscope in field of cell culture at present
Observation, the administration authority account number of cloud server distribution can be just logged in by computer or cell phone application end, is clearly apparent culture in real time
The technology of the situation of change of case inner cell also beyond example.
The content of the invention
In view of the defects of above-mentioned prior art, it is an object of the invention to：Living cells culture network-type in one plants case is provided
Intelligent imaging analysis instrument need not take out culture dish and view and emulate under the microscope, it can be achieved that the non-contact observational study of cell culture, energy
It is enough that clearly observation incubator inner cell culture real-time change situation is realized on computer or mobile phone.
The present invention technical solution be：Living cells culture network-type intelligence imaging analysis instrument in case, including workbench,
The stent of workbench side is arranged on, the microoptic mechanism of bench-top side is fixed on, is connected with microoptic mechanism
And the photoelectronic imaging parts of computer or mobile phone, the objective table above workbench for culture dish holding can be connected, set
In cantilever tip and micro- light source mechanism directly over the objective table, it is fixed on objective table bottom and can turns with dynamic object stage
Dynamic carrier precision rotation platform mechanism, be arranged on carrier precision rotation platform actuator base and can be moved left and right with dynamic object stage
Transverse moving mechanism and be arranged on transverse moving mechanism bottom and can with dynamic object stage move back and forth longitudinal moving mechanism.
Microoptic mechanism, photoelectronic imaging parts and micro- light source mechanism
Further, the top of the objective table is equipped with multiple grooves for culture dish holding, in the objective table
Between be equipped with a through hole.
Further, the shape of the groove is at least one of circular, square.
Further, the carrier precision rotation platform mechanism includes the first stepper motor, installed in the first stepper motor top
The micro- rotating mechanism of planet at end and it is connected with the shaft of the first stepper motor and is used to support the stage carrier of objective table, institute
State and a protective shell is additionally provided with the top of stage carrier, the shaft of protective shell and the first stepper motor is fixed, and the protective shell is worn
The through hole crossed on objective table is simultaneously fixed with objective table, and the top of the protective shell is provided with a fixed knob.
Further, the longitudinal moving mechanism includes fixed supporting table on the table, is fixed on bench-top
Longitudinal stainless steel wire rail, be slidably arranged on longitudinal stainless steel wire rail longitudinal slide unit, be fixed in supporting table and be used for band
Move longitudinal ball screw of longitudinal slide unit anterior-posterior translation and be arranged on stent side and be used to drive longitudinal ball screw to rotate
The second stepper motor, it is described longitudinal direction ball screw be located at workbench top and be located at longitudinal slide unit lower section, described second
The shaft of stepper motor is fixed through stent and with one end of longitudinal ball screw.
Further, the transverse moving mechanism includes being fixed on horizontal stainless steel wire rail at the top of longitudinal slide unit, slides
The horizontal slide unit being arranged on horizontal stainless steel wire rail, the both sides at the top of the longitudinal slide unit are vertically provided with fixed plate and solid
Determine platform, the transverse moving mechanism, which further includes, to be arranged between fixed plate and fixed station and for driving horizontal slide unit or so to translate
Transverse ball lead screw and be fixed on the outside of fixed plate and the 3rd stepper motor for driving transverse ball lead screw to rotate, institute
State the top that transverse ball lead screw is located at horizontal slide unit, the shaft of the 3rd stepper motor through fixed plate and with horizontal ball
One end of screw is fixed.
Further, first stepper motor is fixed on the top of horizontal slide unit.
Further, the microoptic mechanism includes full-automatic Nosepiece, speculum and eyepiece, full-automatic object lens
Multiple object lens are housed, after culture dish is placed with objective table, the light that micro- light source mechanism irradiates is irradiated on converter
On culture dish, light is imaged after object lens and speculum on eyepiece.
Further, the microoptic mechanism is L-type microoptic mechanism.
Further, the photoelectronic imaging parts include ccd image sensor, A/D modular converters and Digital Signal Processing
Module, the ccd image sensor by the video conversion obtained on eyepiece into analog signal, and by A/D modular converters and
Digital signal processing module converts analog signals into the digital signal that can connect mobile phone or computer.
It is using living cells culture network-type intelligence imaging analysis instrument, its advantage in case provided by the present invention：Can
Realize the non-contact observational study of cell culture, i.e., need not take out culture dish and view and emulate under the microscope, can be on computer or mobile phone
Realize and clearly observe incubator inner cell culture real-time change situation, can carry out qualitative and quantitative analysis on cellular level,
The observational studies such as living cell growth graphical analysis, living cells dynamic tracer, the great advantage of the device is without frequently opening training
Support chamber door take out culture dish under the microscope observational study, may be such that culture dish local environment temperature humidity keep be basically unchanged
State, maintains the correctness, reasonability and reliability of cell research, realizes the screen of cell culture real-time monitored research
And mobile.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the side view of the present invention；
Fig. 3 is the partial structural diagram of the present invention；
Fig. 4 is the index path of the microoptic mechanism of the present invention；
Fig. 5 is the structure diagram of the microoptic mechanism of the present invention；
Fig. 6 is the theory structure block diagram of video signal collective/transmission of the present invention；
Fig. 7 is the theory structure block diagram of the photoelectronic imaging parts of the present invention；
Fig. 8 is the circuit diagram of the A/D modular converters of the present invention；
Fig. 9 is the principle of the present invention structure diagram；
The amplification circuit diagram of the photoelectronic imaging parts of Figure 10 present invention；
Figure 11 is the functional block diagram of the Image Data Compression processing of the present invention；
Figure 12 is the Operation interface diagram of the present invention when in use；
Figure 13 is the principle flow chart that the present invention is run；
Figure 14 is the structure diagram of the objective table of the present invention；
Figure 15 is the functional block diagram of the photoelectronic imaging parts of the present invention；
Figure 16 is the schematic diagram that the present invention is put into incubator.
Shown in figure：1- workbench, 2- stents, 3- microoptics mechanism, the full-automatic Nosepieces of 31-, 32- reflections
Mirror, 33- eyepieces, 4- photoelectronic imaging parts, 41-CCD imaging sensors, 42-A/D modular converters, 43- Digital Signal Processing moulds
Block, 5- objective tables, the micro- light source mechanisms of 6-, 7- carrier precision rotation platforms mechanism, the first stepper motors of 71-, the micro- rotation of 72- planets
Mechanism, 73- stage carriers, 8- transverse moving mechanisms, 81- transverse direction stainless steel wire rails, 82- horizontal slide units, 83- transverse direction ball wires
Bar, the 3rd stepper motors of 84-, 9- longitudinal moving mechanisms, 91- supporting tables, 92- longitudinal directions stainless steel wire rail, 93- longitudinal slide units, 94-
Longitudinal ball screw, the second stepper motors of 95-, 10- grooves, 11- through holes, 12- protective shells, 13- fixed knobs, 14- fixed plates,
15- fixed stations.
Embodiment
For it is relatively directly perceived, be fully understood by technical scheme, now just combine attached drawing of the present invention carry out it is non-limiting
Feature description it is as follows：
As shown in Fig. 1-Figure 16, living cells culture network-type intelligence imaging analysis instrument in case, including workbench 1, be arranged on
The stent 2 of 1 side of workbench, the microoptic mechanism 3 for being fixed on 1 top side of workbench, be connected with microoptic mechanism 3 and
The photoelectronic imaging parts 4 of computer or mobile phone, the objective table 5 above workbench 1 for culture dish holding can be connected, set
On 2 top of stent and it is located at the micro- light source mechanism 6 of the surface of objective table 5, is fixed on 5 bottom of objective table and loading can be driven
Carrier precision rotation platform mechanism 7 that platform 5 rotates, be arranged on 7 bottom of carrier precision rotation platform mechanism and can be left with dynamic object stage 5
The transverse moving mechanism 8 that moves right and it is arranged on 8 bottom of transverse moving mechanism and can be moved back and forth with dynamic object stage 5 vertical
To moving mechanism 9.Micro-optics mechanism 3, photoelectronic imaging parts 4 and micro- light source mechanism 6 form microscopic imaging device, i.e., such as Fig. 1
Shown, after forming microscopic imaging device, which is located at the side of bench-top, and the image of device intake is digitized into turning
Change and be transmitted to high in the clouds, be connected by network with computer or mobile phone.
The top of objective table 5 is equipped with multiple grooves 10 for culture dish holding, and the shape of groove 10 is circular, square
At least one of.Certainly, the shape of groove 10 is a kind of better embodiment of the invention for circular, square, specifically
Other shapes, such as ellipse, rectangle etc. can also be used during use.The culture dish of different size can be nested in objective table
In the groove 10 reserved on 5, the size of groove 10 can just make the bottom of culture dish be emerging in the visual model of microoptic mechanism 3
In enclosing.Moreover, objective table 5 can be replaced according to the groove 10 of culture dish holding.
Carrier precision rotation platform mechanism 7 includes the first stepper motor 71, the planet installed in 71 top of the first stepper motor
Micro- rotating mechanism 72 and it is connected with the shaft of the first stepper motor 71 and is used to support the stage carrier 73 of objective table 5, is carried
The top of thing platform stent 73 is additionally provided with a protective shell 12, and protective shell 12 and the shaft of the first stepper motor 71 are fixed, protective shell 12
Fixed through the through hole 11 on objective table 5 and with objective table 5, the top of protective shell 12 is provided with a fixed knob 13.User is led to
The cell imaging of different zones can easily be observed by overregulating the swing of the first stepper motor 71.The micro- rotating mechanism of planet
72 often step further 0.1 degree of rotations.
Supporting table 91 that longitudinal moving mechanism 9 includes being fixed on workbench 1, be fixed on the longitudinal direction at the top of workbench 1 not
Rust steel wire rail 92, the longitudinal slide unit 93 being slidably arranged on longitudinal stainless steel wire rail 92, be fixed in supporting table 91 and be used for band
Move longitudinal ball screw 94 of 93 anterior-posterior translation of longitudinal slide unit and be arranged on 2 side of stent and be used to drive longitudinal ball screw
94 the second stepper motors 95 rotated, longitudinal ball screw 94 are located at the top of workbench 1 and are located at the lower section of longitudinal slide unit 93,
The shaft of second stepper motor 95 is fixed through stent 2 and with one end of longitudinal ball screw 94.
Transverse moving mechanism 8 includes being fixed on the horizontal stainless steel wire rail 81 at the top of longitudinal slide unit 93, is slidably arranged in horizontal stroke
To the horizontal slide unit 82 on stainless steel wire rail 81, the both sides at the top of longitudinal slide unit 93 are vertically provided with fixed plate 14 and fixed station
15, transverse moving mechanism 8, which further includes, to be arranged between fixed plate 14 and fixed station 15 and for driving horizontal slide unit 82 or so flat
The transverse ball lead screw 83 of shifting and it is fixed on the outside of fixed plate 14 and the 3rd step for driving transverse ball lead screw 83 to rotate
Stepper motor 84, transverse ball lead screw 83 are located at the top of horizontal slide unit 82, and the shaft of the 3rd stepper motor 84 passes through fixed plate 14
And fixed with one end of transverse ball lead screw 83.First stepper motor 71 is fixed on the top of horizontal slide unit 82 so that carrier essence
Close turntable mechanism 7 can be connected with transverse moving mechanism 8.
Longitudinal moving mechanism 9 and transverse moving mechanism 8 form two-dimentional platform mechanism, and two-dimentional platform mechanism may be implemented in carrier precision
Turntable mechanism 7 can translate around after fixing, in order to observe all cells on whole two dimensional surface.
As shown in Figure 4 and Figure 5, microoptic mechanism 3 includes full-automatic Nosepiece 31, speculum 32 and eyepiece 33,
After culture dish is placed with objective table 5, full-automatic Nosepiece 31 is equipped with multiple object lens, and micro- light source mechanism 6 is irradiated
Light be irradiated on culture dish, light is imaged after object lens and speculum 32 on eyepiece 33.To reduce this hair as far as possible
The volume of bright whole device, is the L-type light that can be turned round the light path design of light source to microscope imaging according to the refraction principle of light
System.On full-automatic Nosepiece 31 equipped with amplification factor be respectively 5 ×, 10 ×, 20 × and 40 × object lens, carrying out
During cell observation, the timing of full-automatic Nosepiece 31 focuses on automatically can then ensure that for a long time Jiao is flat in photographic process in real time
The stabilization in face and image it is clear.The selection criteria of object lens is：It must be appropriate for working long hours in 37 DEG C or so, humidity 90%
Above, CO2Concentration is in 5% or so cell growth environment, when specifically used, can make full-automatic Nosepiece 31 and mesh
Mirror 33 is arranged to have antifog, defrosting function.
As shown in figure 5, a kind of better embodiment as the present invention, microoptic mechanism 3 is L-type microoptic machine
Structure, i.e. microoptic mechanism 3 are designed to " L " type, and the cell culture situation being imaged passes through the L-type high magnification that specially designs
(can be replaceable according to demand) high-resolution microoptic mechanism by 3 video imagings on photoelectronic imaging parts 4, photoelectronic imaging machine
Part 4 transfer data to special processing unit carry out Image Data Compression processing and be uploaded to Cloud Server be supplied to mobile phone or
Computer carries out tele-experimentation manipulation.
Photoelectronic imaging parts 4 include ccd image sensor 41, A/D modular converters 42 and digital signal processing module 43,
The video conversion obtained on eyepiece 33 into analog signal, and is passed through A/D modular converters 42 and numeral by ccd image sensor 41
Signal processing module 43 converts analog signals into the digital signal that can connect mobile phone or computer, and digital data transmission is to special
Processing unit carry out Image Data Compression processing and be uploaded to Cloud Server being supplied to mobile phone or computer to carry out tele-experimentation behaviour
Control.
In use, the culture dish equipped with cell sample is placed in the groove 10 of objective table 5 first, then pass through first
The position of culture dish where stepper motor 71, the second stepper motor 95 and the 3rd stepper motor 84 make cell to be observed and micro- light
Source mechanism 6, microoptic mechanism 3 full-automatic Nosepiece 31 object lens be adapted to observation angle on, micro- light source machine
The light that structure 6 is sent is LED light line, and it is (red fluorescence, green that micro- light source mechanism 6 can provide white light camera system and triple channel fluorescence
Color fluorescence and blue-fluorescence), can be by suitably adjusting objective table 5 and transverse moving mechanism when observation object changes
8th, longitudinal moving mechanism 9 obtains optimal observation angle；Finally get image information in photoelectronic imaging parts 4, by
Corresponding cellular morphology information can clearly be shown on computer, mobile phone after the digital image processing techniques matched somebody with somebody.
In use, the use environment of the analyzer is unrestricted, i.e., living cells culture network-type intelligence imaging analysis in the case
Instrument fully takes into account the factors such as temperature, humidity, soda acid pH value and the fog in incubator, and resistance to gentle resistance to high humidity design can
Cell culture and continuous observation and the shooting writing function of long-time stable are provided；In terms of translucency：It is high using light transmittance
Eyeglass and 0.17mm heavy sheet glass custom-made Petri dish minimize influence of the environment to collection image definition in case.
In terms of precision controlling：(stepper motor here includes the to computer program control high-precision rotary stepper motor
One stepper motor 71, the second stepper motor 95 and the 3rd stepper motor 84), the rotation pair of 71 driving objective table 5 of the first stepper motor
The position of culture dish to be observed, which is accurately positioned, can reach 0.1 degree；In terms of light source selection：Micro- light source mechanism 6 can provide white light
Camera system and triple channel fluorescence (red fluorescence, green fluorescence and blue-fluorescence), i.e., it is bright to clap, without the time of fluorescence excitation
The problem of difference and heat production；In terms of CCD sensitivity：Scientific grade CCD resolution ratio is up to 10560*10560 reading speeds
800MHz, Pixel Dimensions are 9 μm, and minimum reachable -100 DEG C of cryogenic temperature, high-sensitive CCD makes the light injury of cell smaller；
In terms of image taking speed：Full-automatic Nosepiece 31 and automatic focusing function make image taking speed be less than 500ms, and imaging is more
It hurry up.In terms of image definition：The match use of imaging technique of synchrotron radiation light source compensate for light microscopic and cannot observe directly carefully
The defects of born of the same parents' pattern and the structural relation of tissue.
As shown in fig. 7, it is the theory structure block diagram of photoelectronic imaging parts 4, ccd image sensor 41 will be on eyepiece 33
Obtained video conversion turns analog signal into analog signal, and by A/D modular converters 42 and digital signal processing module 43
The digital signal of mobile phone or computer can be connected by changing into, and digital data transmission carries out Image Data Compression to special processing unit
Handling and being uploaded to Cloud Server is supplied to mobile phone or computer to carry out tele-experimentation manipulation.As described in Figure 8, it is A/D moduluss of conversion
The circuit diagram of block.As indicated at 11, its be row Image Data Compression functional block diagram.
As shown in fig. 6, its video signal collective/transmission structure block diagram for the present invention, photoelectronic imaging parts 4 get figure
As information, corresponding cellular morphology information can clearly be shown on computer, mobile phone after matched digital image processing techniques
Show.As described in Figure 9, corresponding cellular morphology information is transferred to by WiFi network after matched digital image processing techniques
Mobile phone or computer.
Certainly, a better embodiment as the present invention, as shown in figure 12, carrier precision rotation platform mechanism 7 and two
The upper computer software of Wei Tai mechanisms (including transverse moving mechanism 8 and longitudinal moving mechanism 9) is the control and computing of whole equipment
Center a, it is desirable to provide operation interface easy to use, it is necessary to which internal complicated control algolithm is isolated.According to the behaviour of user
Make, upper computer software accesses equipment and control panel by modules, and provides feedback of status over the display
Information and generation real-time curve.The system software mainly includes the control module of the operation interface of user, equipment and control panel, figure
As processing module.
According to the demand of user, software needs to be controlled each ancillary equipment, while will also be in computer display
The upper real-time status feedback information of display, so that operating personnel understand the working condition of equipment in time in real time, ensures whole system
Safely and steadily run.The software of living cells culture network-type intelligence imaging analysis instrument includes image analysis processing in whole case
Module, device control module and interpersonal interactive module, its hierarchical chart are as shown in figure 12.Human-computer interaction module carries for user
For easily interactive environment, user selectes test assignment, and inputs required information, and program automatically forms testing process, and adjusts
With the relevant module in backstage.Analyzed more than, the operational process of intelligent living cells culture imaging system software is as shown in figure 13.
The interface of program is largely divided into three parts, first, control menu：The menu can recall the parameter setting process of ancillary equipment, together
When can open and close electron assistant line function on CCD display windows；Second, procedure operation part：The bootable use in the part
Family is sequentially completed each several part, so as to fulfill the observation of cell is treated；Third, display portion：The part includes CCD display windows, shape
Coordinate on state display window, program power unit numerical value display window and status bar is shown.
Moreover, as shown in figure 16, cell incubator compared with the prior art, such cell incubator must be used micro-
Mirror, and the microscope of the prior art is directly anchored in cell incubator or is arranged on outside incubator, there is position
The problem of adjusting suffering is put, limitation is larger, and living cells culture network-type intelligence imaging analysis in case provided by the present invention
Instrument is a complete special equipment, and microoptic mechanism 3 is directly provided in this equipment, this equipment is direct when using
Be put into incubator box body can, it is convenient to take out.Moreover, cell incubator of the present invention relative to the prior art, it has
There is intelligent network, because it can connect mobile phone or computer uses, clearly observation training can be realized on computer or mobile phone
Case inner cell culture real-time change situation is supported, facilitates the observational study of people, and the cell incubator of these prior arts institute
Do not possess.
Living cells culture network-type intelligence imaging analysis instrument in case provided by the present invention, when use, are set directly at cell
As a kind of special device, it can be achieved that the non-contact observational study of cell culture, i.e., need not take out culture dish aobvious in incubator
Viewed and emulated under micro mirror, clearly observation incubator inner cell culture real-time change situation can be realized on computer or mobile phone, can be into
The observational studies such as qualitative and quantitative analysis, living cell growth graphical analysis, living cells dynamic tracer on row cellular level, this is
The great advantage of system be without frequently open culture chamber door take out culture dish under the microscope observational study, may be such that culture dish institute
The temperature humidity of place's environment keeps being basically unchanged state, maintains the correctness, reasonability and reliability of cell research, realizes
The screen and mobile of cell culture real-time monitored research.
Certainly, the foregoing is merely a prefered embodiment of the invention, and therefore, it does not limit the scope of the claims of the present invention, all fortune
With description of the invention and schema content institute, simply modification and equivalent structure change for it, should all similarly be contained in the special of the present invention
Within sharp protection domain.
Claims (9)
1. living cells culture network-type intelligence imaging analysis instrument in case, it is characterised in that：Including workbench, it is arranged on workbench one
The stent of side, the microoptic mechanism for being fixed on bench-top side, be connected with microoptic mechanism and can connect computer
Or mobile phone photoelectronic imaging parts, above workbench for culture dish holding objective table, be arranged on cantilever tip and position
Micro- light source mechanism directly over objective table, be fixed on objective table bottom and can be with the accurate rotation of carrier that dynamic object stage rotates
Turntable mechanism, be arranged on carrier precision rotation platform actuator base and can with the transverse moving mechanism that dynamic object stage moves left and right with
And it is arranged on transverse moving mechanism bottom and the longitudinal moving mechanism that can be moved back and forth with dynamic object stage；
The carrier precision rotation platform mechanism includes the first stepper motor, the micro- rotation of planet installed in the first stepper motor top
Mechanism and it is connected with the shaft of the first stepper motor and is used to support the stage carrier of objective table, the stage carrier
Top is additionally provided with a protective shell, and the shaft of protective shell and the first stepper motor is fixed, and the protective shell passes through logical on objective table
Kong Bingyu objective tables are fixed, and the top of the protective shell is provided with a fixed knob.
2. living cells culture network-type intelligence imaging analysis instrument in case according to claim 1, it is characterised in that：The load
The top of thing platform is equipped with multiple grooves for culture dish holding, and the centre of the objective table is equipped with a through hole.
3. living cells culture network-type intelligence imaging analysis instrument in case according to claim 2, it is characterised in that：It is described recessed
The shape of groove is at least one of circular, square.
4. living cells culture network-type intelligence imaging analysis instrument in case according to claim 1, it is characterised in that：It is described vertical
Include fixed supporting table on the table to moving mechanism, be fixed on longitudinal stainless steel wire rail of bench-top, slide and set
The longitudinal slide unit on longitudinal stainless steel wire rail is put, is fixed in supporting table and is used for the longitudinal direction for driving longitudinal slide unit anterior-posterior translation
Ball screw and it is arranged on stent side and the second stepper motor for driving longitudinal ball screw to rotate, longitudinal direction rolling
Ballscrew is located at the top of workbench and positioned at the lower section of longitudinal slide unit, the shaft of second stepper motor through stent and with
One end of longitudinal ball screw is fixed.
5. living cells culture network-type intelligence imaging analysis instrument in case according to claim 4, it is characterised in that：The horizontal stroke
Include being fixed on horizontal stainless steel wire rail at the top of longitudinal slide unit to moving mechanism, be slidably arranged on horizontal stainless steel wire rail
Horizontal slide unit, the both sides at the top of the longitudinal slide unit are vertically provided with fixed plate and fixed station, and the transverse moving mechanism is also
Including being arranged between fixed plate and fixed station and being used for transverse ball lead screw and the fixation for driving horizontal slide unit or so to translate
On the outside of fixed plate and the 3rd stepper motor for driving transverse ball lead screw to rotate, the transverse ball lead screw are located at laterally
The top of slide unit, the shaft of the 3rd stepper motor are fixed through fixed plate and with one end of transverse ball lead screw.
6. living cells culture network-type intelligence imaging analysis instrument in case according to claim 5, it is characterised in that：Described
One stepper motor is fixed on the top of horizontal slide unit.
7. living cells culture network-type intelligence imaging analysis instrument in case according to claim 6, it is characterised in that：It is described aobvious
Micro-optics mechanism includes full-automatic Nosepiece, speculum and eyepiece, and multiple object lens are housed on full-automatic Nosepiece, when
After being placed with culture dish on objective table, the light that micro- light source mechanism irradiates is irradiated on culture dish, and light is through object lens and instead
It is imaged after penetrating mirror on eyepiece.
8. living cells culture network-type intelligence imaging analysis instrument in case according to claim 7, it is characterised in that：It is described aobvious
Micro-optics mechanism is L-type microoptic mechanism.
9. living cells culture network-type intelligence imaging analysis instrument in case according to claim 7, it is characterised in that：The light
Electrical imaging parts include ccd image sensor, A/D modular converters and digital signal processing module, and the ccd image sensor will
The video conversion obtained on eyepiece will be simulated by A/D modular converters and digital signal processing module and believed into analog signal
Number it is converted into connecting the digital signal of mobile phone or computer.
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| CN107749936A (en) * | 2017-10-12 | 2018-03-02 | 武汉互创联合科技有限公司 | A kind of imaging system for embryo, egg mother cell or stem cell |
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| CN109355177A (en) * | 2018-11-29 | 2019-02-19 | 苏州度奕星科技有限公司 | A kind of living cells monitoring device |
| CN110184187A (en) * | 2019-07-08 | 2019-08-30 | 无锡瑞生医疗科技有限公司 | The online scope of minicell culture |
| CN110551632B (en) * | 2019-07-25 | 2021-02-05 | 南方医科大学珠江医院 | Real-time monitoring cell culture system and culture method thereof |
| CN111592986B (en) * | 2020-05-27 | 2024-03-19 | 南京兰伯艾克斯生物科技有限公司 | Intelligent culture and remote observation culture device and method |
| CN112080424B (en) * | 2020-09-05 | 2022-05-17 | 湖南师范大学 | Monitoring device for cell culture |
| CN114609770B (en) * | 2022-04-18 | 2024-03-26 | 福州海关技术中心 | Auxiliary device for improving microscopic imaging effect of plant seeds and working method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001228088A (en) * | 2000-02-18 | 2001-08-24 | Nippon Laser & Electronics Lab | Optical scanning apparatus for living body specimen |
| JP2003161893A (en) * | 2002-03-22 | 2003-06-06 | Olympus Optical Co Ltd | Micro die section apparatus |
| CN2867371Y (en) * | 2005-09-12 | 2007-02-07 | 桂林电子工业学院 | Automatic inverted microscope |
| CN101553749A (en) * | 2006-08-04 | 2009-10-07 | 伊康尼西斯公司 | Dynamic scanning automatic microscope and method |
| CN204964362U (en) * | 2015-08-17 | 2016-01-13 | 宁波键一电子信息技术有限公司 | Incasement cell culture network type intelligence formation of image analysis appearance alive |
-
2015
- 2015-08-17 CN CN201510535321.6A patent/CN105136665B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001228088A (en) * | 2000-02-18 | 2001-08-24 | Nippon Laser & Electronics Lab | Optical scanning apparatus for living body specimen |
| JP2003161893A (en) * | 2002-03-22 | 2003-06-06 | Olympus Optical Co Ltd | Micro die section apparatus |
| CN2867371Y (en) * | 2005-09-12 | 2007-02-07 | 桂林电子工业学院 | Automatic inverted microscope |
| CN101553749A (en) * | 2006-08-04 | 2009-10-07 | 伊康尼西斯公司 | Dynamic scanning automatic microscope and method |
| CN204964362U (en) * | 2015-08-17 | 2016-01-13 | 宁波键一电子信息技术有限公司 | Incasement cell culture network type intelligence formation of image analysis appearance alive |
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