CN114609770B - Auxiliary device for improving microscopic imaging effect of plant seeds and working method thereof - Google Patents
Auxiliary device for improving microscopic imaging effect of plant seeds and working method thereof Download PDFInfo
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- CN114609770B CN114609770B CN202210405736.1A CN202210405736A CN114609770B CN 114609770 B CN114609770 B CN 114609770B CN 202210405736 A CN202210405736 A CN 202210405736A CN 114609770 B CN114609770 B CN 114609770B
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- 238000003384 imaging method Methods 0.000 title claims abstract description 69
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- 230000007246 mechanism Effects 0.000 claims abstract description 18
- 238000010586 diagram Methods 0.000 claims description 7
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- 230000008859 change Effects 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims description 6
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- 230000002349 favourable effect Effects 0.000 abstract description 2
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- 238000005286 illumination Methods 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
- G02B21/245—Devices for focusing using auxiliary sources, detectors
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
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Abstract
The invention relates to an auxiliary device for improving microscopic imaging effect of plant seeds and a working method thereof, the device comprises a bracket which is arranged around the periphery of an objective table, wherein a plurality of light adjusting mechanisms are uniformly arranged on the bracket along the circumferential direction, a plurality of layers of light source mounting frames which can move up and down are vertically arranged on the light adjusting mechanisms, light sources are arranged on the light source mounting frames, the light source on the light source mounting frame at the lowest layer can rotate along a horizontal axis so as to lean against the objective table obliquely and irradiate the objective table, and then the shadow at the bottom of a photographed object is eliminated through refraction of light rays in glass of the objective table. The device is favorable for improving the definition of seed microscopic imaging and enhancing the stereoscopic imaging effect of seed microscopic imaging.
Description
Technical Field
The invention belongs to the technical field of microscopic imaging, and particularly relates to an auxiliary device for improving a microscopic imaging effect of plant seeds and a working method thereof.
Background
The stereoscopic microscope is a microscope with positive stereoscopic impression. The method is widely applied to morphological feature observation of plant seeds, and becomes an essential key instrument for plant morphological research. In the morphological detection and identification process of plant seeds at the port, microscopic morphological characteristics of the seeds need to be observed through a stereoscopic microscope so as to judge the types of the plant seeds. Taking clear microscopic pictures plays a vital role in seed specimen preservation, species identification and related scientific research. Although the structure of the current stereoscopic microscope is perfect, the definition of microscopic imaging of plant seeds is not high and the imaging is lack of stereoscopic impression due to the manual operation factors of the instrument and a photographer. Therefore, further improvement of the microscopic imaging definition of plant seeds under a stereoscopic microscope and enhancement of the stereoscopic imaging effect are an overwhelming trend. How to improve the microscopic imaging definition of plant seeds and to enhance the stereoscopic imaging effect is also an urgent problem to be solved at present.
The current stereoscopic microscope has the following problems: the configuration light source is fixed on the objective lens or the bracket of the microscope, and is mostly provided with single light source or double light sources with the same direction, the light emitted by the configuration light source irradiates the weed seed sample on the objective table, then the weed seed sample enters the imaging system through the reflection of the objective lens, and finally the obtained imaging information is transmitted to the control computer. Light rays in a single direction or two different directions can easily cause that the partial part of the seed sample can not receive the light rays completely or partially, so that an image of the partial part of the seed obtained on a control computer is unclear or has shadows, and the definition of microscopic imaging of the seed is directly affected; the single light source or the peripheral light source is configured to uniformly adjust the brightness, so that the seed microscopic image has poor stereoscopic imaging effect.
Disclosure of Invention
The invention aims to provide an auxiliary device for improving the microscopic imaging effect of plant seeds and a working method thereof, and the device is favorable for improving the definition and the stereoscopic imaging effect of microscopic imaging of the seeds.
In order to achieve the above purpose, the invention adopts the following technical scheme: the utility model provides an improve auxiliary device of microscopic imaging effect of plant seed, includes the support of enclosing to locate objective table week portion, the support evenly is provided with a plurality of light adjustment mechanism along circumference, light adjustment mechanism is provided with the light source mounting bracket that the multilayer can reciprocate along vertical, installs the light source on each light source mounting bracket, and the light source on the light source mounting bracket of the lowest floor can be rotatory along the horizontal axis to lean on the objective table and shine to the objective table to one side, and then through the refraction of light in objective table glass, eliminate the shade of being shot the thing bottom.
Further, the left end and the right end of the lamplight adjusting mechanism are respectively provided with a vertical sliding rail, each layer of light source mounting frame is horizontally arranged between the two vertical sliding rails, and one side or two sides of the lamplight adjusting mechanism are provided with a ball screw pair and a driving motor thereof so as to drive the light source mounting frame to move up and down along the vertical sliding rails; the light source mounting frame at the lowest layer is provided with a horizontal rotating frame capable of rotating along a horizontal shaft and is driven to rotate by a rotating motor, and the light source is mounted on the horizontal rotating frame to rotate under the driving of the light source mounting frame.
Further, the LED lamp comprises a power supply unit and a control unit, wherein the power supply unit is respectively and electrically connected with each light source, the driving motor and the rotating motor so as to supply power to the light source, the driving motor and the rotating motor; the control unit is respectively and electrically connected with the power supply unit, each light source, the driving motor and the rotating motor, so as to flexibly control the light emission of each light source and adjust the optical parameters, the irradiation height and the irradiation angle of each light source.
Further, the light source is a light tube arranged horizontally or a plurality of point light sources arranged in an array along the horizontal direction, and the plurality of point light sources can be simultaneously or independently controlled to switch and adjust optical parameters.
Further, the bracket is formed by connecting a plurality of lateral frames, and each light adjusting mechanism is respectively arranged on the corresponding lateral frame; the adjacent lateral frames are detachably connected, so that the support can be opened laterally, and the object stage is enclosed in the support.
Further, the bracket is of a quadrilateral structure formed by connecting four lateral frames, and each lateral frame is provided with the light adjusting mechanism; the adjacent side frames are detachably hinged, so that the number of the side frames is increased or reduced according to the requirement, and the brackets with different polygonal structures are flexibly formed.
Further, the objective table is of a two-layer glass structure, the lower layer is a fixed bottom layer glass plate, the bottom layer glass plate is a black opaque plate, a white opaque plate or transparent glass, and the upper layer is high-light-transmittance glass capable of horizontally moving.
The invention also provides a working method of the auxiliary device for improving the microscopic imaging effect of the plant seeds, which is characterized by comprising the following steps:
step S1, after a seed is placed on an objective table, automatically adjusting a light source mounting frame at the lowest layer to a set height according to the type, shape and size information of the seed and the shape and size information of the objective table, and simultaneously rotating a light source to an initial angle and imaging; then observing shadow parts in the imaging diagram, and continuously adjusting the height of the light source mounting frame and the rotation angle of the light source so as to eliminate shadows generated by photographed seeds on the objective table;
s2, observing the brightness of the top surface and the periphery of the seed in the imaging graph, when the brightness is lower than the brightness of the bottom surface of the seed, starting the light sources on the light source mounting frames on the upper layers, and continuously adjusting the positions of the light sources through the corresponding light source mounting frames to enable the light sources to irradiate on different positions of the seed; stopping moving the light source position when the brightness of the top surface of the seed is the same as that of other parts;
and S3, observing the definition and the stereoscopic impression of the whole imaging of the seeds, respectively adjusting the brightness of the light sources in all directions of each layer according to the observation result, and stopping adjusting the light sources when the image reaches the optimal display state.
Further, the control unit of the auxiliary device is connected with the imaging device; in the imaging process, the control unit acquires an imaging image from the imaging device in real time, correlates the imaging image with the position state information of each light source, and then performs the following processing in the adjustment process:
in step S1, comparing and analyzing the change of the shadow part in the imaging diagram, and determining the height of the mounting frame of the lowest light source and the selection angle of the light source with the minimum shadow in the imaging diagram as the target;
in step S2, comparing and analyzing the change of the brightness difference of different parts of the seeds, and determining the positions of the light sources of the upper layers by taking the minimum brightness difference of different parts of the seeds as a target;
in step S3, the contrast analysis is performed on the sharpness and the stereoscopic impression of the whole seed imaging, and the brightness of the light sources in each layer and each direction is determined with the imaging sharpness and the stereoscopic impression being the strongest target.
Compared with the prior art, the invention has the following beneficial effects: the device is provided with an adjusting structure capable of flexibly adjusting illumination intensity, height and angle around an objective table, so that shadows of all angles of a photographed object (photographed plant seed) are effectively eliminated, and definition and third dimension of microscopic imaging of a stereoscopic microscope are greatly improved. In addition, the device has ingenious structural design, is simple and convenient to operate and is easy to assemble and disassemble. Therefore, the invention has strong practicability and wide application prospect.
Drawings
Fig. 1 is a schematic side view of an embodiment of the present invention.
Fig. 2 is a schematic top view of an embodiment of the present invention.
Fig. 3 is a control schematic of an embodiment of the present invention.
Fig. 4 is a schematic view showing an opened state of the stand in the embodiment of the present invention.
Detailed Description
The invention will be further described with reference to the accompanying drawings and examples.
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
As shown in fig. 1 and 2, this embodiment provides an auxiliary device for improving microscopic imaging effect of plant seeds, including a support 1 surrounding the periphery of an objective table, the support is uniformly provided with a plurality of light adjusting mechanisms 2 along the circumferential direction, the light adjusting mechanisms 2 are vertically provided with a plurality of light source mounting frames 3 capable of moving up and down, each light source mounting frame is provided with a light source 4, a light source 401 on a light source mounting frame 301 at the lowest layer can rotate along a horizontal axis so as to lean against the objective table 5 and irradiate the objective table 5, and then the shadow of the bottom of a photographed object is eliminated through refraction of light rays in the glass of the objective table.
In this embodiment, the two left and right ends of the light adjusting mechanism 2 are respectively provided with a vertical sliding rail 6, each layer of light source mounting frame 3 is horizontally installed between two vertical sliding rails 6, and one side or two sides of the light source mounting frame is provided with a ball screw pair and a driving motor 9 thereof so as to drive the light source mounting frame to move up and down along the vertical sliding rails 6; the light source mounting frame 301 at the lowest layer is provided with a horizontal rotating frame 8 which can rotate along a horizontal axis and is driven to rotate by a rotating motor, and the light source 401 is mounted on the horizontal rotating frame 8 to rotate under the driving of the horizontal rotating frame.
As shown in fig. 3, the device further comprises a power supply unit and a control unit, wherein the power supply unit is respectively and electrically connected with each light source, the driving motor and the rotating motor so as to supply power to the light source, the driving motor and the rotating motor; the control unit is respectively and electrically connected with the power supply unit, each light source, the driving motor and the rotating motor so as to independently control the light emission of each light source and adjust the optical parameters, the irradiation height and the irradiation angle of each light source. In the preferred embodiment, function keys can be provided to set the usual light source switch and adjustment to a fixed option, like a layer of light source is turned on or off at the same time, so that the operation convenience of the user can be increased.
According to practical needs, the light source can be a lamp tube arranged horizontally or a plurality of point light sources arranged in an array along the horizontal direction, and the point light sources can be simultaneously or independently controlled by switching and optical parameter adjustment. In this embodiment, the light source uses a horizontal lamp.
In this embodiment, the bracket 1 is formed by connecting a plurality of lateral frames 101, and each light adjusting mechanism 2 is respectively mounted on the corresponding lateral frame 101; the adjacent lateral frames 101 are detachably connected so that the support can be opened laterally to enclose the object table in the support. In this embodiment, the bracket 1 is a quadrilateral structure formed by connecting four side frames 101, and each side frame 101 is provided with the light adjusting mechanism 2; the adjacent side frames are detachably hinged, so that the number of the side frames is increased or reduced according to the requirement, and the brackets with different polygonal structures are flexibly formed. The open state of the stand 1 is shown in fig. 4.
In this embodiment, the objective table 5 is a two-layer glass structure, the upper layer is a high light-transmitting glass capable of moving horizontally, the lower layer is a fixed bottom glass plate, and the bottom glass plate may be a black opaque plate, a white opaque plate or a transparent glass, and one of them may be selected for fixation.
The embodiment also provides a working method of the auxiliary device for improving the microscopic imaging effect of the plant seeds, which comprises the following steps:
step S1, after a seed is placed on an objective table, automatically adjusting a light source mounting frame at the lowest layer to a set height according to the type, shape and size information of the seed and the shape and size information of the objective table, and simultaneously rotating a light source to an initial angle and imaging; then observing the shadow part in the imaging diagram, and continuously adjusting the height of the light source mounting frame and the rotation angle of the light source so as to eliminate the shadow generated by the photographed seeds on the objective table.
S2, observing the brightness of the top surface and the periphery of the seed in the imaging graph, when the brightness is lower than the brightness of the bottom surface of the seed, starting the light sources on the light source mounting frames on the upper layers, and continuously adjusting the positions of the light sources through the corresponding light source mounting frames to enable the light sources to irradiate on different positions of the seed; and stopping moving the light source position when the brightness of the top surface of the seed is the same as that of other parts.
And S3, observing the definition and the stereoscopic impression of the whole imaging of the seeds, respectively adjusting the brightness of the light sources in all directions of each layer according to the observation result, and stopping adjusting the light sources when the image reaches the optimal display state.
In a preferred embodiment of the invention, the control unit of the auxiliary device is connected with the imaging device, and the auxiliary device realizes automatic adjustment through the control unit. In the imaging process, the control unit acquires an imaging image from the imaging device in real time, correlates the imaging image with the position state information of each light source, and then performs the following processing in the adjustment process:
in step S1, the change of the shadow part in the imaging chart is compared and analyzed, and the height of the lowest light source mounting frame and the selection angle of the light source are determined with the aim of minimum shadow in the imaging chart.
In step S2, the change in the difference between the brightnesses of the different portions of the seed is compared and analyzed, and the positions of the light sources in the upper layers are determined with the minimum difference between the brightnesses of the different portions of the seed as a target.
In step S3, the contrast analysis is performed on the sharpness and the stereoscopic impression of the whole seed imaging, and the brightness of the light sources in each layer and each direction is determined with the imaging sharpness and the stereoscopic impression being the strongest target.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the invention in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to the equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims (7)
1. The working method of the auxiliary device for improving the microscopic imaging effect of the plant seeds is characterized by comprising a bracket which is arranged on the periphery of an objective table in a surrounding manner, wherein a plurality of light adjusting mechanisms are uniformly arranged on the bracket along the circumferential direction, a plurality of layers of light source mounting frames capable of moving up and down are vertically arranged on the light adjusting mechanisms, light sources are arranged on the light source mounting frames, the light source on the light source mounting frame on the lowest layer can rotate along a horizontal axis to lean against the objective table and irradiate the objective table, and then the shadow at the bottom of a photographed object is eliminated through the refraction of light rays in the glass of the objective table;
the working method of the auxiliary device for improving the microscopic imaging effect of the plant seeds comprises the following steps:
step S1, after a seed is placed on an objective table, automatically adjusting a light source mounting frame at the lowest layer to a set height according to the type, shape and size information of the seed and the shape and size information of the objective table, and simultaneously rotating a light source to an initial angle and imaging; then observing shadow parts in the imaging diagram, and continuously adjusting the height of the light source mounting frame and the rotation angle of the light source so as to eliminate shadows generated by photographed seeds on the objective table;
s2, observing the brightness of the top surface and the periphery of the seed in the imaging graph, when the brightness is lower than the brightness of the bottom surface of the seed, starting the light sources on the light source mounting frames on the upper layers, and continuously adjusting the positions of the light sources through the corresponding light source mounting frames to enable the light sources to irradiate on different positions of the seed; stopping moving the light source position when the brightness of the top surface of the seed is the same as that of other parts;
s3, observing the definition and the stereoscopic impression of the integral imaging of the seeds, respectively adjusting the brightness of each layer of azimuth light source according to the observation result, and stopping adjusting the light source when the image reaches the optimal display state;
the control unit of the auxiliary device is connected with the imaging device; in the imaging process, the control unit acquires an imaging image from the imaging device in real time, correlates the imaging image with the position state information of each light source, and then performs the following processing in the adjustment process:
in step S1, comparing and analyzing the change of the shadow part in the imaging diagram, and determining the height of the mounting frame of the lowest light source and the selection angle of the light source with the minimum shadow in the imaging diagram as the target;
in step S2, comparing and analyzing the change of the brightness difference of different parts of the seeds, and determining the positions of the light sources of the upper layers by taking the minimum brightness difference of different parts of the seeds as a target;
in step S3, the contrast analysis is performed on the sharpness and the stereoscopic impression of the whole seed imaging, and the brightness of the light sources in each layer and each direction is determined with the imaging sharpness and the stereoscopic impression being the strongest target.
2. The working method of the auxiliary device for improving the microscopic imaging effect of the plant seeds according to claim 1, wherein the left end and the right end of the light adjusting mechanism are respectively provided with a vertical sliding rail, each layer of light source mounting frame is horizontally arranged between the two vertical sliding rails, and one side or two sides of the light source mounting frame is provided with a ball screw pair and a driving motor thereof so as to drive the light source mounting frame to move up and down along the vertical sliding rails; the light source mounting frame at the lowest layer is provided with a horizontal rotating frame capable of rotating along a horizontal shaft and is driven to rotate by a rotating motor, and the light source is mounted on the horizontal rotating frame to rotate under the driving of the light source mounting frame.
3. The method of claim 2, comprising a power supply unit and a control unit, wherein the power supply unit is electrically connected to each light source, the driving motor and the rotating motor respectively to supply power to the light sources, the driving motor and the rotating motor; the control unit is respectively and electrically connected with the power supply unit, each light source, the driving motor and the rotating motor, so as to flexibly control the light emission of each light source and adjust the optical parameters, the irradiation height and the irradiation angle of each light source.
4. The method of claim 1, wherein the light source is a horizontally arranged lamp tube or a plurality of point light sources arranged in an array along a horizontal direction, and the plurality of point light sources can be simultaneously or individually controlled for switching and optical parameter adjustment.
5. The method of claim 1, wherein the bracket is formed by connecting a plurality of side frames, and each light adjusting mechanism is respectively arranged on the corresponding side frame; the adjacent lateral frames are detachably connected, so that the support can be opened laterally, and the object stage is enclosed in the support.
6. The method for improving the microscopic imaging effect of plant seeds according to claim 5, wherein the bracket is a quadrilateral structure formed by connecting four side frames, and each side frame is provided with the light adjusting mechanism; the adjacent side frames are detachably hinged, so that the number of the side frames is increased or reduced according to the requirement, and the brackets with different polygonal structures are flexibly formed.
7. The method of claim 1, wherein the stage has a two-layer structure, the lower layer is a fixed bottom glass plate, the bottom glass plate is a black opaque plate, a white opaque plate or transparent glass, and the upper layer is a high light-transmitting glass capable of moving horizontally.
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| CN202210405736.1A CN114609770B (en) | 2022-04-18 | 2022-04-18 | Auxiliary device for improving microscopic imaging effect of plant seeds and working method thereof |
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| CN202210405736.1A CN114609770B (en) | 2022-04-18 | 2022-04-18 | Auxiliary device for improving microscopic imaging effect of plant seeds and working method thereof |
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| CN114609770B true CN114609770B (en) | 2024-03-26 |
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