CN107479178B - Full-automatic microscope scanning system without object carrying table - Google Patents
Full-automatic microscope scanning system without object carrying table Download PDFInfo
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- CN107479178B CN107479178B CN201710739634.2A CN201710739634A CN107479178B CN 107479178 B CN107479178 B CN 107479178B CN 201710739634 A CN201710739634 A CN 201710739634A CN 107479178 B CN107479178 B CN 107479178B
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/33—Immersion oils, or microscope systems or objectives for use with immersion fluids
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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Abstract
The invention discloses a full-automatic microscope scanning system without a carrying table, which comprises a microscope module, a slide cabin module, a slide moving module, a dipping oil dripping module, an objective lens conversion module, a visual field conversion module, an electric appliance control module and a computer. The invention greatly improves the quality of morphological examination results by detecting and analyzing the morphologies of visible components such as complex and variable cells, and avoids misdiagnosis and missed diagnosis caused by overlooking morphological examination due to excessive dependence on automatic equipment; compared with the traditional microscopic analysis, the automatic degree and the working efficiency are greatly improved, the artificial interference is reduced, and the analysis precision is higher.
Description
Technical Field
The invention relates to the technical field of medical clinical examination, in particular to a full-automatic microscope scanning system without a loading table, which can automatically identify a target object, automatically carry out the whole microscopic examination process according to set steps and time, can accurately and accurately transmit, judge and read, is safe and quick, is environment-friendly and saves human resources.
Background
The morphological examination is the basis of clinical examination and laboratory diagnosis, is an important part of blood and body fluid examination, and is widely applied to various fields of clinical diagnosis, pathological cell histology, biology, pharmaceutical chemistry, genetic genes and the like. Is the most classical, most direct, most effective and most economical means for disease diagnosis and differential diagnosis, even the "gold standard" for disease diagnosis. With the development of natural science, medical examination is developed from original manual operation to computer-controlled automatic operation, but due to complexity and variability of morphology of visible components such as cells, an automatic instrument cannot completely recognize the visible components, and error analysis or omission occurs in many cases, so that morphological identification and confirmation still need to be performed under a microscope. Moreover, due to the wide application of antibiotics, the number of cells of the organism is not changed greatly when the organism suffers from certain diseases, but the morphological change is obvious, so that the judgment needs to be made by blood cell morphological examination. With the rapid development of science and technology, the application of automation and electronic instruments and the like in clinical examination enriches detection methods and improves examination efficiency, so that part of examinees think that biochemical instrument analysis can replace microscopic examination, and in addition, the shortage of the examinees and the pursuit of examination result timeliness lead the examinees to excessively depend on automation equipment and ignore morphological examination, thereby influencing examination quality, causing missed diagnosis, misdiagnosis and even medical dispute. And correspondingly careful under-mirror morphological observation can provide a favorable basis for clinical diagnosis. The traditional manual control microscope cannot meet the requirements of clinical and clinical laboratories, and a full-automatic control microscope with the action accurately controlled by a computer is urgently needed.
Disclosure of Invention
The invention provides a full-automatic microscope scanning system without a carrying table, which is used for solving the problems that the existing automatic instrument cannot completely identify the shapes of complex and variable visible components such as cells and the like and the traditional manual control microscope mode cannot meet the requirements of clinics and clinical laboratories on efficiency, precision and the like.
The technical scheme of the invention is as follows:
a full-automatic microscope scanning system without a carrying table comprises: the microscope comprises a microscope module, a slide cabin module, a slide moving module, a lens oil dripping module, an objective lens conversion module, a visual field conversion module, a control module and a computer.
Wherein the microscope module is used for transmitting the image in the slide to the computer through the microscope and the digital camera; the slide bin module is used for storing slides; the slide moving module is used for taking and placing the slide from the slide bin, transmitting the slide to the oil dropping level and the microscope observation position and adjusting the focal length; the immersion lens oil dripping module is used for dripping immersion lens oil onto a glass slide needing oil dripping; the objective lens conversion module is used for converting microscope objective lenses with different multiplying powers; the field conversion module is used for converting different microscope fields; the control module is used for controlling the slide moving module, the immersion lens oil dripping module, the objective lens conversion module and the visual field conversion module to act; the computer is used for sending instructions to the control module. The computer is also provided with an image processing knowledge base for analyzing the images to obtain a detection conclusion.
As a preferable scheme of the microscope module, the microscope module comprises a microscope, a high definition camera adapter and a digital camera. The microscope is fixed on the bottom plate, the high-definition camera adapter is fixed on the microscope eyepiece base, the digital camera is connected with the high-definition camera adapter, and a data line of the digital camera is connected to the computer.
As an optimized proposal of the slide bin module, the slide bin module comprises a right vertical plate, a rebound self-locking device, a guide rail, a slide block, a bracket, a slide bin limit groove, a slide bin and a limit block. The rebound self-locking device is fixed on the right vertical plate and is divided into an upper part and a lower part which are used for locking or popping up the slide bin limiting groove; the guide rail is fixed in the middle of the right vertical plate and divided into an upper part and a lower part; the sliding block is connected to the guide rail, and the bracket is fixed on the sliding block; the slide bin limiting groove is fixed on the bracket and used for placing the slide bin; the slide bin is used for storing slides; the limiting block is fixed on the right vertical plate and used for preventing the upper and lower two slide bin limiting grooves from exceeding the boundary when being ejected out.
As a preferable mode of the above slide moving module, the slide moving module includes an X-axis control device, a Y-axis control device, a Z-axis control device, a cantilever, and an electric clamp. The X-axis control device is fixed on the bottom plate and used for moving the cantilever in the horizontal direction; the Y-axis control device is fixed on a movable bracket of the X-axis control device and is used for moving the cantilever in the front-back direction; the Z-axis control device is fixed on a movable support of the Y-axis control device and is used for moving the cantilever in the vertical direction; the cantilever is fixed on a movable bracket of the Z-axis control device and used for supporting the electric clamp; the electric clamp is fixed on the cantilever and used for clamping and placing the glass slide.
As an optimal scheme of the immersion lens oil dripping module, the immersion lens oil dripping module comprises an oil dripping pump, an oil dripping pipe, a support and an oil dripping needle. The oil dropping pump is fixed on the back of the right vertical plate, and the immersion oil is sucked to the oil dropping needle from the bottle through the oil dropping pipe and finally dropped onto the glass sheet; the oil dripping needle is fixed on the bracket, and the bracket is fixed above the right vertical plate.
As a preferred scheme of the objective lens conversion module, the objective lens conversion module comprises a stepping motor fixing seat, a stepping motor, a synchronous belt, an objective lens gear ring, a synchronous wheel fixing frame and a synchronous wheel; the bottom surface of the stepping motor fixing seat is fixed on the bottom plate, and the left side of the stepping motor fixing seat is fixed on the left vertical plate and used for supporting the stepping motor; the synchronizing wheel fixing frame is fixed on the bottom plate and used for supporting the synchronizing wheel; the synchronous belt is connected with the stepping motor, the synchronous wheel and the objective gear ring, so that the objective can be freely switched.
As a preferred scheme of the above vision field conversion module, the vision field conversion module comprises a fixed seat, a screw motor, a bracket and a vision field plugboard; the fixed seat is fixed on the bottom plate and used for supporting the screw rod motor; the screw rod motor is fixed on the fixed seat and used for moving the visual field conversion module left and right; the bracket is fixed on the screw rod motor slide block; the visual field inserting plate is fixed on the bracket.
As a preferable scheme of the control module, the control module includes a switching power supply, a main control circuit board, a wiring board, and a relay control board. The switching power supply is fixed on the back of the rear vertical plate and used for supplying power to all electric devices; the main control circuit board is fixed on the back of the vertical plate and used for controlling the work of each electric device; the wiring board is fixed on the side surface of the left vertical plate and used for switching the main control circuit board and the lines of all electric devices; the relay control board is fixed on the back of the vertical plate and used for receiving the instruction of the main control circuit board to control the opening and closing operations of the electric clamp.
The computer is used for sending instructions to the control module so as to control the slide moving module, the immersion lens oil dripping module, the objective lens conversion module and the visual field conversion module to act; the computer is also provided with an image processing knowledge base which is used for storing data and images and analyzing the images to obtain a detection conclusion.
The full-automatic microscope scanning system without the object carrying table can be widely applied to identification of various samples in a clinical laboratory, remote medical pathological consultation, high-precision microscopic measurement, automatic acquisition and identification of microscopic targets and the like. The invention greatly improves the quality of morphological examination results by detecting and analyzing the morphologies of visible components such as complex and variable cells, and avoids misdiagnosis and missed diagnosis caused by overlooking morphological examination due to excessive dependence on automatic equipment; compared with the traditional microscopic analysis, the degree of automation and the working efficiency are greatly improved, the artificial interference is reduced, and the analysis precision is higher.
Drawings
The invention is described below with reference to the accompanying drawings:
FIG. 1 is a schematic view of the entire structure of embodiment 1;
FIG. 2 is a schematic structural view of a microscope module in example 1;
FIG. 3 is a schematic view of the structure of the slide magazine module in example 1;
FIG. 4 is a schematic block diagram of a slide moving module according to embodiment 1;
FIG. 5 is a schematic structural view of a immersion lens oil dropping module in example 1;
FIG. 6 is a schematic structural view of an objective lens conversion module according to embodiment 1;
fig. 7 is a schematic structural diagram of a field conversion module in embodiment 1;
fig. 8 is a schematic structural diagram of a control module in embodiment 1;
in the figure, 1-microscope module; 46-a base plate; 8-a microscope; 9-high definition camera adapter; 10-a digital camera; 2-slide magazine module; 11-a right vertical plate; 12-rebound self-locking device; 13-a guide rail; 14-a slide block; 15-a scaffold; 16-slide bin limiting groove; 17-slide bin; 18-a limiting block; 3-a slide moving module; 19-X axis control; 20-Y axis control means; 21-Z axis control means; 22-a cantilever; 23-electric clamp; 4-immersion lens oil dripping module; 24-a drop oil pump; 25-oil drip pipe; 26-a scaffold; 27-oil-dripping needle; 5-objective lens conversion module; 28-step motor fixing seat; 29-a stepper motor; 30-a synchronous belt; 31-objective ring gear; 32-a synchronizing wheel fixing frame; 33-a synchronizing wheel; 34-a limiting photoelectric fixing piece; 35-limiting photoelectric; 6-field of view conversion module; 36-a fixed seat; 37-a screw motor; 38-a scaffold; 39-field plate; 7-a control module; 40-a switching power supply; 41-a main control circuit board; 42-patch panel; 43-relay control panel; 44-a left vertical plate; 45-rear vertical plate.
Detailed Description
The present invention will be further described with reference to the following specific examples.
Example 1
Fig. 1 shows an embodiment of a stage-free fully automatic microscope scanning system, which includes a microscope module 1, a slide chamber module 2, a slide moving module 3, a dipping oil dropping module 4, an objective lens conversion module 5, a field conversion module 6, a control module 7 and a computer.
Wherein the microscope module is used for transmitting the image in the slide to the computer through the microscope and the digital camera. As shown in fig. 2, the microscope module includes a microscope 8, a high definition camera interface 9, and a digital camera 10; wherein, microscope 8 is fixed on bottom plate 46, and high definition camera adapter 9 is fixed on the microscope trinocular seat, and digital camera 10 is connected with high definition camera adapter 9, and the data line of digital camera 10 is connected to in the computer. The microscope with more control functions can also automatically control and adjust the field diaphragm, the objective lens, the condenser lens, the aperture and the like.
The slide magazine module is used for storing slides. As shown in fig. 3, the slide chamber module comprises a right vertical plate 11, a rebound self-locking device 12, a guide rail 13, a slide block 14, a bracket 15, a slide chamber limiting groove 16, a slide chamber 17 and a limiting block 18; wherein, the right vertical plate 11 is fixed on the bottom plate 46; the rebound self-locking device 12 is fixed on the right vertical plate and divided into an upper part and a lower part for locking or popping up the slide bin limiting groove 16; the guide rail 13 is fixed in the middle of the right vertical plate 11 and divided into an upper part and a lower part; the sliding block 14 is connected to the guide rail 13, and the bracket 15 is fixed on the sliding block 14; the slide bin limiting groove 16 is fixed on the bracket 15 and used for placing the slide bin 17; the slide magazine 17 is used for storing slides; and a limiting block 18 is fixed on the right vertical plate 11 and is used for preventing the upper and lower slide bin limiting grooves 16 from exceeding the boundary when being ejected.
The slide moving module is used for taking and putting the slide from the slide bin, conveying the slide to the drop oil level and the microscope observation position and adjusting the focal distance. As shown in fig. 4, the slide moving module includes an X-axis control device 19, a Y-axis control device 20, a Z-axis control device 21, a cantilever 22, and an electric clamp 23; wherein, the X-axis control device 19 is fixed on the bottom plate 46 and used for moving the cantilever in the horizontal direction, and three positions of photoelectricity are arranged; the Y-axis control device 20 is fixed on the movable support of the X-axis control device 19 and used for moving the cantilever in the front-back direction; the Z-axis control device 21 is fixed on a movable bracket of the Y-axis control device 20 and is used for moving the cantilever in the vertical direction; the cantilever 22 is fixed on a movable bracket of the Z-axis control device 21 and used for supporting the electric clamp; an electric clamp 23 is fixed to the cantilever 22 for clamping a slide. The automatic control of two cantilever shafts and a third focusing shaft is realized through the X, Y, Z shaft three-way automatic control platform.
And the immersion lens oil dripping module is used for dripping the immersion lens oil onto the glass slide needing oil dripping. As shown in fig. 5, the immersion lens oil dripping module comprises an oil dripping pump 24, an oil dripping pipe 25, a bracket 26 and an oil dripping needle 27; wherein, the oil dripping pump 24 is fixed on the back of the right vertical plate 11, the immersion oil is absorbed to the oil dripping needle 27 from the bottle through the oil dripping pipe 25, and finally the immersion oil is dripped on the glass sheet; the oil dripping needle 27 is fixed on the bracket 26, and the bracket 26 is fixed above the right vertical plate 11.
The objective lens conversion module is used for converting microscope objective lenses with different magnifications. As shown in fig. 6, the objective lens conversion module includes a stepping motor fixing base 28, a stepping motor 29, a synchronous belt 30, an objective lens gear ring 31, a synchronous wheel fixing frame 32, a synchronous wheel 33, a limit photoelectric fixing piece 34, and a limit photoelectric piece 35; wherein, the bottom surface of the step motor fixing seat 28 is fixed on the bottom plate 46, and the left side is fixed on the left vertical plate and used for supporting the step motor 29; the synchronizing wheel fixing frame 32 is fixed on the bottom plate and is used for supporting the synchronizing wheel 33; the synchronous belt 30 is connected with the stepping motor 29, the synchronous wheel 33 and the objective ring gear 31 to realize free switching of the objective; the limit photoelectric fixing sheet 34 is fixed on the stepping motor fixing seat 28; the limiting photoelectric cell 35 is fixed on the limiting photoelectric fixing sheet 34.
The field of view conversion module is used for converting different microscope fields of view. As shown in fig. 7, the visual field conversion module includes a fixing base 36, a lead screw motor 37, a bracket 38, and a visual field plug board 39; the fixed seat 36 is fixed on the bottom plate 46 and used for supporting the screw rod motor; the screw motor 37 is fixed on the fixed seat 36 and used for moving the vision field conversion module left and right; the bracket 38 is fixed on the slide block of the screw motor 37; the vision board 39 is fixed to the bracket 38.
The control module is used for controlling the slide moving module, the immersion lens oil dripping module, the objective lens conversion module and the visual field conversion module to act. As shown in fig. 8, the control module includes a switching power supply 40, a main control circuit board 41, a wiring board 42, and a relay control board 43; wherein, the switching power supply 40 is fixed on the back of the rear vertical plate 45 and used for supplying power to all electric devices; the main control circuit board 41 is fixed on the back of the rear vertical plate 45 and is used for controlling the work of each electric device; the wiring board 42 is fixed on the side surface of the left vertical plate 44 and is used for switching the main control circuit board and the lines of various electric devices; the relay control board 43 is fixed on the back of the back vertical board 45 and used for receiving the instruction of the main control circuit board to control the opening and closing operation of the electric clamp.
The computer is used for sending instructions to the control module so as to control the actions of the slide moving module, the immersion lens oil dripping module, the objective lens conversion module and the visual field conversion module; the computer is also provided with an image processing knowledge base which is used for storing data and images and analyzing the images to obtain a detection conclusion.
The invention greatly improves the quality of morphological examination results by detecting and analyzing the morphologies of visible components such as complex and variable cells, and avoids misdiagnosis and missed diagnosis caused by overlooking morphological examination due to excessive dependence on automatic equipment. Compared with the traditional mode, the invention has the advantages of reducing the man-made interference and improving the analysis precision, and greatly improves the automation degree and the working efficiency.
Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.
Claims (7)
1. The utility model provides a full-automatic microscope scanning system of no thing platform, its characterized in that: the microscope comprises a microscope module, a slide bin module, a slide moving module, a dipping oil dripping module, an objective lens conversion module, a visual field conversion module, a control module and a computer; wherein the microscope module is used for transmitting the image in the slide to a computer through a microscope and a digital camera; the slide bin module is used for storing slides; the slide moving module is used for taking and placing slides from the slide bin, conveying the slides to a drop oil level and a microscope observation position and adjusting focal length; the immersion lens oil dripping module is used for dripping immersion lens oil onto a glass slide needing oil dripping; the objective lens conversion module is used for converting microscope objective lenses with different multiplying powers; the field conversion module is used for converting different microscope fields; the control module is used for controlling the actions of the slide moving module, the immersion lens oil dripping module, the objective lens conversion module and the visual field conversion module; the computer is used for sending instructions to the control module so as to control the actions of the slide moving module, the immersion lens oil dripping module, the objective lens conversion module and the visual field conversion module; the slide bin module comprises a right vertical plate, a rebound self-locking device, a guide rail, a slide block, a bracket, a slide bin limiting groove, a slide bin and a limiting block; the right vertical plate is fixed on the bottom plate; the rebound self-locking device is fixed on the right vertical plate and divided into an upper part and a lower part for locking or popping up the slide bin limiting groove; the guide rail is fixed in the middle of the right vertical plate and divided into an upper part and a lower part; the sliding block is connected to the guide rail, and the bracket is fixed on the sliding block; the slide bin limiting groove is fixed on the bracket and used for placing the slide bin; the slide chamber is used for storing slides; the limiting block is fixed on the right vertical plate and used for preventing the upper and lower slide bin limiting grooves from exceeding the boundary when being ejected; the slide moving module comprises an X-axis control device, a Y-axis control device, a Z-axis control device, a cantilever and an electric clamp; the X-axis control device is fixed on the bottom plate and used for moving the cantilever in the horizontal direction; the Y-axis control device is fixed on a movable bracket of the X-axis control device and is used for moving the cantilever in the front-back direction; the Z-axis control device is fixed on a movable support of the Y-axis control device and is used for moving the cantilever in the vertical direction; the cantilever is fixed on a movable bracket of the Z-axis control device and used for supporting the electric clamp; the electric clamp is fixed on the cantilever and used for clamping and placing the glass slide.
2. The stage-less fully automated microscope scanning system of claim 1, wherein: the computer is also provided with an image processing knowledge base which is used for storing data and images and analyzing the images to obtain a detection conclusion.
3. The stage-less fully automated microscope scanning system of claim 1, wherein: the microscope module comprises a microscope, a high-definition camera adapter and a digital camera; wherein the microscope is fixed on the bottom plate; the high-definition camera adapter is fixed on the microscope eyepiece base; the digital camera is connected with the high-definition camera adapter interface, and a data line of the digital camera is connected to the computer.
4. The stage-less fully automated microscope scanning system of claim 1, wherein: the immersion lens oil dripping module comprises an oil dripping pump, an oil dripping pipe, a bracket and an oil dripping needle; the oil dropping pump is fixed on the back of the right vertical plate, and the immersion oil is sucked to the oil dropping needle from the bottle through the oil dropping pipe and finally dropped onto the glass sheet; the oil dripping needle is fixed on the bracket, and the bracket is fixed above the right vertical plate.
5. The stage-less fully automated microscope scanning system of claim 1, wherein: the objective lens conversion module comprises a stepping motor fixing seat, a stepping motor, a synchronous belt, an objective lens gear ring, a synchronous wheel fixing frame and a synchronous wheel; the stepping motor fixing seat is fixed on the bottom plate and used for supporting the stepping motor; the synchronizing wheel fixing frame is fixed on the bottom plate and used for supporting the synchronizing wheel; the synchronous belt is connected with the stepping motor, the synchronous wheel and the objective gear ring, so that the objective can be freely switched.
6. The stage-less fully automated microscope scanning system of claim 1, wherein: the visual field conversion module comprises a fixed seat, a screw rod motor, a bracket and a visual field plugboard; the fixed seat is fixed on the bottom plate and used for supporting the screw rod motor; the screw rod motor is fixed on the fixed seat and used for moving the visual field conversion module left and right; the bracket is fixed on the screw rod motor slide block; the vision inserting plate is fixed on the bracket.
7. The stage-less fully automated microscope scanning system of claim 1, wherein: the control module comprises a switching power supply, a main control circuit board, a wiring board and a relay control board; the switching power supply is fixed on the back face of the rear vertical plate and used for supplying power to all electric devices; the main control circuit board is fixed on the back of the vertical plate and used for controlling the work of each electric device; the wiring board is fixed on the side face of the left vertical plate and used for switching the main control circuit board and the lines of all electric devices; the relay control board is fixed on the back of the vertical plate and used for receiving instructions of the main control circuit board to control the opening and closing operations of the electric clamp.
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| CN109358420B (en) * | 2018-12-05 | 2024-07-02 | 成都朴华科技有限公司 | Intelligent microscope |
| CN111220510A (en) * | 2020-01-15 | 2020-06-02 | 深圳市生强科技有限公司 | Full-automatic blood cell morphology scanning system |
| CN113916751B (en) * | 2021-09-15 | 2024-02-09 | 中国人民解放军总医院第一医学中心 | Automatic detection system for whole cells of micro-vision image |
| CN114035312A (en) * | 2021-10-12 | 2022-02-11 | 李昕昱 | Three-axis automatic objective table capable of adapting to multiple microscopes |
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| CN103364937A (en) * | 2013-07-11 | 2013-10-23 | 四川美生科技有限公司 | Full-automatic check analysis microscope |
| CN204188877U (en) * | 2014-08-15 | 2015-03-04 | 杭州卓腾信息技术有限公司 | Based on the panoramic scanning device of the micro-image of photoetching slide |
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Denomination of invention: A fully automatic microscope scanning system without a stage Effective date of registration: 20231109 Granted publication date: 20230321 Pledgee: Industrial and Commercial Bank of China Limited Jinan Licheng Branch Pledgor: SHANDONG STARS BIOINDUSTRY CO.,LTD. Registration number: Y2023370000129 |