CN101126699B - Pollen grain image data collecting system - Google Patents
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- CN101126699B CN101126699B CN2007101752176A CN200710175217A CN101126699B CN 101126699 B CN101126699 B CN 101126699B CN 2007101752176 A CN2007101752176 A CN 2007101752176A CN 200710175217 A CN200710175217 A CN 200710175217A CN 101126699 B CN101126699 B CN 101126699B
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Abstract
The utility model relates to an image data acquisition system for pollen particles, which comprises an electrical worktable system, an image pickup module, an image transmission module, an illuminating system, a display module, and a central control system; wherein, the electrical worktable system is used to control the movement of the pollen particles samples and enable a microscope to focus on the pollen particles; the image pickup module is used to acquire and transform the amplified image of the pollen particles samples shown in the microscope into image files; the image transmission module is used to convey the image files transformed by the image pickup module to the central control system; the illuminating system is used for lighting when the microscope is focused on the pollen particles samples; the display module is used to show the whole acquisition process; the central control system is used to control the movement of the electrical worktable system, the lighting of the illuminating system, and show the amplified image of the pollen particles samples and the transformed image files in the display module, meanwhile the transformed image files are stored, thus realizing the image data acquisition of the pollen particles. The utility model solves the current problems of low efficiency in pollen analyzing and monitoring, and the inability to guarantee the accuracy of the pollen monitoring results by manpower.
Description
Technical field
The present invention relates to pollen granule sub-image data collecting field, relate in particular to a kind of pollen granule sub-image data acquisition system (DAS).
Background technology
Pollen is the male sex-cell of plant, can be divided into anemophily, entomophila, hydrophily pollen etc. with circulation way, and anemophilous pollen is also referred to as gas and passes pollen.When pollen was propagated in air, some people just can produce allergic reaction suck pollen in respiratory after, i.e. pollinosis (Pollinosis).The professional is according to for many years experience, and summed up 5 standards judging sensitization pollen: 1) pollen amount is big, and content is higher in air; 2) the pollen volume is little, is easy to suck respiratory tract; 3) great majority pass pollen for gas; 4) plant that produces this kind pollen grows extensively; 5) pollen contains and can cause irritated antigen, and this is the most important point.It is the important composition composition of atmosphere bioaerosol that gas passes pollen, be easy in air, propagate, when passing, gas just can cause the popular of pollinosis when sensitization pollen reaches finite concentration, thereby influence the normal life of Susceptible population, even jeopardize patient's life, cause inestimable loss to social economy to a certain extent.Entomophila and hydrophily pollen are seldom propagated in air, can not cause the popular of pollinosis, and therefore, the monitoring of gas biography pollen concentration is extremely important.
Current main employing settling methods is gathered pollen, carries out pollen classification and statistical counting with artificial microscopy method then.So-called artificial microscopy method is exactly the pollen print after experienced observation person conscientiously observes 400 times of amplifications of Photobiology microscopically line by line, manually differentiates the pollen granule subcategory, the number and the pollen frequency of the different pollen of complicate statistics.Mainly there is following shortcoming in artificial microscopy method:
1) observation person's labour intensity is bigger, easily causes physical fatigue, thereby reduces monitoring accuracy;
2) observer's observation technology is had relatively high expectations, need can grasp the microscopic morphology feature of different pollen particles exactly through long-term constantly technical training, correctly discern pollen, one has staff redeployment, the new pollen observer of necessary training;
3) monitoring result is subjected to artificial factor, and the monitoring result difference that different observers draw is bigger;
4) send out the peak period at pollen, longer because of reading the sheet time under the mirror, easily cause observer's eyes muscular fatigue, influence counting and work efficiency.
Because there is above-mentioned shortcoming in current techniques, makes that current artificial microscopy method is not high to the efficient of pollen monitoring analysis, and be difficult to guarantee the accuracy rate of pollen monitoring result, therefore the current technical scheme of needing a kind of pollen granule sub-image data acquisition system (DAS) badly.
Summary of the invention
Technical matters to be solved by this invention provides a kind of pollen granule sub-image data acquisition system (DAS), solves current artificial not highly to the efficient of pollen monitoring analysis, and is difficult to guarantee the problem of the accuracy rate of pollen monitoring result.
In order to address the above problem, the invention provides a kind of pollen granule sub-image data acquisition system (DAS), comprise microscope, electric table system, photographing module, image transmission module, illuminator, central control system, and air pressure module, wherein,
The electric table system is used to control moving of pollen granule subsample, and microscope is focused on the pollen granule subsample;
Photographing module is used to catch the magnified image of the shown pollen granule subsample of microscope, and is converted into image file;
Image transmission module is used for giving central control system with the image file transfers of photographing module conversion;
Illuminator, the illumination when being used for microscope focusing pollen granule subsample comprises the reflection source all around that direction of illumination is downward;
Display module is used to show the process of whole collection;
Central control system, be used for to the electric table system carry out motion control, to the illuminator control of throwing light on, and the image file after the magnified image of the pollen granule subsample that microscope is shown and the conversion shows on display module, image file after the storage conversion is simultaneously finished the collection of pollen granule sub-image data;
The air pressure module is used for when microscope focuses on the pollen granule subsample, by the control of central control system, forms a positive air pressure on the pollen granule subsample, comes particle in the air between filtering pollen granule subsample and the microscopical object lens.
Further, said system also can comprise, described electric table system comprises three electric tables, motion-control module;
Three electric tables are used for mobile pollen granule subsample and make microscope focus on the pollen granule subsample;
Motion-control module is used for controlling and drive the displacement of three electric tables on X, Y, three directions of Z axle by keyboard or JOG control enclosure.
Further, said system can comprise that also described photographing module comprises camera, image capture module, wherein,
Camera is used to catch the magnified image of the shown pollen granule subsample of microscope;
Image capture module is used to obtain the image of camera collection, and is converted into image file.
Further, said system can comprise that also described camera comprises CCD camera or CMOS camera.
Further, said system can comprise that also described illuminator also comprises transmitted light source, the On/Off of central control system control light source and the direction of illumination of regulating and controlling light source and the power of light source and the light wave that makes each light emitted different wave length.
Further, said system can comprise that also the downward reflection source all around of described direction of illumination is installed in around the camera, and upwards the described transmitted light source of projection is placed in three electric tables.
Further, said system can comprise that also described air pressure module is installed in the micro objective side, contains gas outlet in the air pressure module, and gas outlet is parallel to the micro objective side, guarantees to form on the pollen granule subsample positive air pressure vertically downward.
Further, said system also can comprise, the opening and closing of described central control system control air pressure module and the power of the control positive air pressure pressure that forms.
Further, said system can comprise that also described central control system comprises memory module, and memory module is used for the file that whole gatherer process produces is stored.
For prior art, use the present invention and have the following advantages:
Use three electric tables of precision optical machinery with manual or automated manner control pollen print displacement, utilize realtime graphic demonstration and acquisition system to show and collection pollen particle micro-imaging with manual or automated manner, can alleviate monitoring personnel's labour intensity to a great extent, improve automation degree of equipment.
Description of drawings
Fig. 1 is the structural drawing of pollen granule sub-image data acquisition system (DAS) in the specific embodiment of the invention;
Fig. 2 is the schematic appearance of pollen granule sub-image data acquisition system (DAS) in the specific embodiment of the invention;
Fig. 3 is the schematic appearance of three electric tables in the specific embodiment of the invention;
Fig. 4 is the process flow diagram of a kind of pollen granule sub-image collecting method in the specific embodiment of the invention.
Embodiment
The invention will be further described below in conjunction with the drawings and specific embodiments.
A kind of pollen granule sub-image data acquisition system (DAS) and analytic system are that light harvesting, mechanical, electrical, computer image processing technology are in high precision, the robotization micro image collection system of one.This system mainly utilizes three electric tables of precision optical machinery, there is pollen granule subsample (promptly gas passes the print of pollen particle) to carry out high magnification optics amplification imaging by microscope (optical microscope) to collection, and the pollen granule sub-image after will amplifying through the CCD camera system deposits hard disc of computer in, and the applies image analysis system analyzes the object image data then.But this system's range of application is wider, and it can be used for the analysis of raindrop particle diameter spectrum, the analysis of air contaminant particles spectrum etc., and this system is mainly used in collection and the analysis that realizes pollen granule sub-image data at present.
Divide according to function, this system mainly is made up of the two large divisions: pollen granule sub-image data acquisition system (DAS) and pollen granule sub-image data analysis system.
Fig. 1 has described the structural drawing of pollen granule sub-image data acquisition system (DAS), and its outward appearance comprises microscope, electric table system, photographing module, image transmission module as shown in Figure 2, illuminator, and air pressure module, display module, central control system, wherein,
The electric table system is used to control moving of pollen granule subsample, and microscope is focused on the pollen granule subsample; The electric table system comprises three electric tables, motion-control module;
Three electric tables, its outward appearance are used for mobile pollen granule subsample and make microscope focus on the pollen granule subsample as shown in Figure 3;
Motion-control module is used for controlling and drive the displacement of three electric tables on X, Y, three directions of Z axle by keyboard or JOG control enclosure;
Photographing module is used to catch the magnified image of the shown pollen granule subsample of microscope, and is converted into image file; Photographing module comprises camera, image capture module;
Camera is used to catch the magnified image of the shown pollen granule subsample of microscope;
Camera comprises CCD camera or CMOS camera.
Image capture module is used to obtain the image of camera collection, and is converted into image file.
Image transmission module is used for giving central control system with the image file transfers of photographing module conversion;
Illuminator, illumination when being used for microscope focusing pollen granule subsample, comprise the downward a plurality of reflection sources of direction of illumination all around, reach the upwards transmitted light source of projection, central control system can be controlled the On/Off of light source, and the power of the direction of illumination of regulating and controlling light source and light source and the light wave that makes each light emitted different wave length; Wherein the downward a plurality of all around reflection sources of direction of illumination can be installed in around the camera, and upwards the transmitted light source of projection can be placed in three electric tables of pollen granule subsample;
Provide illuminator can reach good illuminating effect and image sample clearly, improve the image sampling resolution.
Trickle object is when amplification imaging, and its highest resolution depends on the wavelength of the light wave that is reflected, and wavelength is short more, and resolution is just high more, and the light source of illuminator of the present invention can be launched short wavelength's light wave here.
The air pressure module is used for when microscope focuses on the pollen granule subsample, by the control of central control system, forms a positive air pressure on the pollen granule subsample;
The air pressure module is installed in the micro objective side, contains gas outlet in the air pressure module, and gas outlet is parallel to the micro objective side, guarantees to form on the pollen granule subsample positive air pressure vertically downward.
By the air pressure module can filtering pollen granule subsample and microscopical object lens between air in particle, improve sampling degree of accuracy and resolution.
Central control system can be controlled the opening and closing of air pressure module and the power of the control positive air pressure pressure that forms.
Display module is used to show the process of whole collection;
Central control system, be used for control to whole pollen granule sub-image data acquisition, comprise the electric table system is carried out motion control, illuminator thrown light on control and the On/Off of control air pressure module and the power that produces positive air pressure pressure, and the image file after the magnified image of the pollen granule subsample that microscope is shown and the conversion shows on display module, image file after the storage conversion is simultaneously finished the collection of pollen granule sub-image data; Central control system comprises memory module;
Memory module is used for the file that whole gatherer process produces is stored.
The invention will be further described below in conjunction with instantiation.
In this example, pollen granule sub-image data acquisition system (DAS) comprises continuous adjustable focus microscope, three electrical measuring instruments of contactless micro-imaging, and the CCD camera, image pick-up card, illuminator, the positive pressure air pump, computing machine, wherein,
The electric table system is that three electrical measuring instruments of contactless micro-imaging, motion-control module are that three electric table drive systems (comprising stepper drive motors and motion control card), central control system are that computing machine, image capture module are that image pick-up card (abbreviation capture card), air pressure module are the positive pressure air pump.
The adjustable focus microscope can be an electronic automatic continuous zoom microscope continuously; Enlargement ratio can be realized the 75-750x continuous vari-focus for 75-750x (1.5-15x);
Three electrical measuring instruments of wherein contactless micro-imaging comprise, comprise three electric tables, three electric table drive systems (comprising stepper drive motors and motion control card), electrical measuring instrument Control Software (abbreviation Control Software);
Three electric table drive systems are used for and computing machine exchange command and parameter, and drive three electric tables and move;
The mobile control of three electric tables is divided into manually and electronic two kinds; Can realize Z axle manual coarse adjustment and fine setting by Z axle focusing handwheel.The electrical measuring instrument Control Software normally starts after the operation, can realize the electricity driving displacement of worktable on X, Y, three directions of Z axle by keyboard or JOG control enclosure.
The electronic bearing accuracy of three electric tables is higher, and concrete parameter is as follows:
.X, Y, Z axle bearing accuracy:＜=1～3um
.X, Y-axis repetitive positioning accuracy: 0.5～1um
.Z axle is measured repetitive positioning accuracy:＜=1～3um
. table stroke (i.e. observation and moving range): X＜=100mm, Y＜=100mm
. glass platform size: 180mm*180mm
Three electronic movings range of electric table Z axle are littler than manual moving range, should be earlier during focal plane in the location with the focusing handwheel thick focal plane of harmonizing, and then the electricity consumption flowing mode carries out accurate focal plane and regulates.
The Keyboard Control mode: the Keyboard Control button ←, →, ↑, ↓ four keys can control three electric tables and move on X-Y plane; Two keys of PageUp, PageDn are used to control electric table moving on Z-direction.
JOG control enclosure control mode: the control handle on the rotation JOG control enclosure panel, when making it point to the PC sign, the JOG control enclosure can not be controlled the motion of three worktable, by three working table movement of computer control, is " √ " before the control enclosure in the upper right corner, this computer-chronograph master interface indication JOG; Control handle on the rotating panel when making it point to the JOG sign, has only the JOG control enclosure can control the motion of three worktable, and computing machine can not controlled three working table movement, is " * " before the control enclosure indication JOG in the upper right corner, this computer-chronograph master interface.When JOG controls when effective, click the X+ on the control enclosure panel, X-, Y+, Y-, Z+, the Z-button can be controlled the motion of three worktable respectively.
After bringing to focus and choosing image position, just can carry out the pollen granule sub-image and observe and image acquisition with the electrical measuring instrument Control Software.
Three electric tables are used for focusing on and the moving target thing in the automatic or manual mode.
Transmitted light source is installed in three electric tables in the illuminator, and reflection source is installed in around the CCD camera;
Reflection source can be a soft fiber cold light, and parameter can be 12V100W, around continuous adjustable focus microscope camera lens, is used for opaque article and observes.
Transmitted light source, parameter can be 6V20W, in table base, is used for transparent substance and observes.
The positive pressure air pump is installed in the micro objective side, and gas outlet is parallel to the micro objective side, guarantees to form on the pollen granule subsample positive air pressure vertically downward;
The CCD camera links to each other with microscope by C type interface, links to each other with image pick-up card by the CS-232 interface, is used to catch microscopical magnified image;
Image pick-up card, motion control card are inserted in respectively in the PCI slot of computing machine (being PC) main frame, and Control Software is installed on computers.Computing machine can be controlled illuminator and positive pressure air pump in sampling process, the image that the CCD camera obtains passes to computing machine and saves as image file via image pick-up card; Via three electric table drive system exchange commands and parameter, realized control between Control Software and three electric tables to non-contact 3-D image electrical measuring instrument.
Can show CCD institute capturing video in real time by computing machine; Can set the electronic mobile step distance of X, Y, three directions of X; Can adjust the position of three electric tables, make three shaft mechanicals all get back to true origin; Can also be with manual or automated manner memory image file etc.
Fig. 4 has described a kind of flow process of pollen granule sub-image collecting method, may further comprise the steps,
Step 110, open system are provided with the parameter of three electrical measuring instruments of contactless micro-imaging, start computing machine three electrical measuring instruments of contactless micro-imaging are controlled;
After normal unlatching of system's all hardware equipment and the operation, start computing machine three electrical measuring instruments of contactless micro-imaging are controlled.
Step-length when computing machine can be set three electric table keyboard fast moving by the fast moving step-length.The keyboard fast moving is exactly when pinning Ctrl, pin three electric tables keyboard control key ' ←, →, ↑, ↓, PageUp or PageDn ', three electric tables will be with ' fast moving step-length ' fast moving.
Computing machine can be set the stroke range of electronic focusing.
Pollen granule increment sheet is placed on the glass plate of three electric tables, and place the microscope camera lens under, microscope enlargement factor handle places 5 places, regulate the Z axle to the layer at pollen particle place with focusing handwheel, re-use keyboard and carry out the precision focusing, and regulate transmitted light source and reflection source, make the pollen particle on the display present image clearly;
Set the sampling frame number, select store path, import primary filename, set initial serial number, set sampling interval,, start the automatic continuous acquisition function of several pollen granule sub-images by ' beginning ' button.
Current pollen granule sub-image is the starting point of continuous acquisition, gathers (X to totalframes * Y to totalframes) individual file altogether with fixing stepping from the off, and filename is with basic name of file and file sequence number composition.The sampling interval time set time interval between three double displacements of electric table.
Three electric tables fixedly step distance can be selected by the drop-down button under the red point in the upper right corner, software master interface.
Can see moving of three electric tables in the automatic continuous acquisition process of several pollen granule sub-images, when the collection of continuous pollen granule sub-image is finished, three electric tables are retracted the start position before the continuous acquisition and are stopped to move, and finish the automatic continuous acquisition of several pollen granule sub-images.
Be the test figure result who obtains by the present invention below.
Table 1. test figure statistics
| The print sequence number | Picture number (width of cloth) | True pollen number | Read accurate number | Misread number | The skip number |
| 1 | 150 width of cloth | 1925 | 1783 | 76 | 141 |
| 2 | 100 width of cloth | 1891 | 1703 | 95 | 190 |
| 3 | 119 width of cloth | 93 | 78 | 6 | 15 |
| Amount to | 369 width of cloth | 3909 | 3564 | 177 | 346 |
The total accuracy of reading rate of table 2. is analyzed
| The print sequence number | Read accurate rate | Misread rate | The skip rate | The statistics accuracy rate |
| 1 | 93％ | 4％ | 7％ | 97％ |
| 2 | 90％ | 5％ | 10％ | 95％ |
| 3 | 84％ | 6％ | 16％ | 90％ |
| On average | 89％ | 5％ | 11％ | 94％ |
From above-mentioned test figure as can be seen, use the present invention and carry out pollen discriminating and statistics, realized the function of automatic statistics pollen frequency, more than total counting number rate of accuracy reached to 70%, reduced the error of human factor.
Use three electric tables of precision optical machinery with manual or automated manner control pollen print displacement, utilize realtime graphic demonstration and acquisition system to show and collection pollen particle micro-imaging with manual or automated manner, can alleviate monitoring personnel's labour intensity to a great extent, improve automation degree of equipment.
The above; only for the preferable specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and anyly is familiar with the people of this technology in the disclosed technical scope of the present invention; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claim.
Claims (4)
- 4. system as claimed in claim 3 is characterized in that, described camera comprises CCD camera or CMOS camera.
- 5. the system as claimed in claim 1, it is characterized in that described air pressure module is installed in the micro objective side, contains gas outlet in the air pressure module, gas outlet is parallel to the micro objective side, guarantees to form on the pollen granule subsample positive air pressure vertically downward.
- 6. system as claimed in claim 5 is characterized in that, the opening and closing of described central control system control air pressure module and the power of the control positive air pressure pressure that forms.
- 7. the system as claimed in claim 1 is characterized in that, described central control system comprises memory module, and memory module is used for the file that whole gatherer process produces is stored.
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| CN2007101752176A CN101126699B (en) | 2007-09-27 | 2007-09-27 | Pollen grain image data collecting system |
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| CN103196912A (en) * | 2013-04-09 | 2013-07-10 | 中国烟草总公司郑州烟草研究院 | Microcosmic characterization method for smoke aerosol particles in cellosilk surface accumulation process and capturing and shooting device |
| CN105444670A (en) * | 2015-12-02 | 2016-03-30 | 库天下（北京）信息技术有限公司 | Remote detection and identification system and method for high-end consumer goods |
| CN107664642A (en) * | 2017-11-01 | 2018-02-06 | 航天新长征大道科技有限公司 | A kind of apparent flaws device for fast detecting |
| CN110251078A (en) * | 2019-01-30 | 2019-09-20 | 北京大学第三医院（北京大学第三临床医学院） | Microscopical imaging method, imaging system and microscope |
| CN115220132A (en) * | 2022-07-04 | 2022-10-21 | 山东浪潮智慧医疗科技有限公司 | Method for forecasting pollen concentration in atmosphere |
| CN115202026A (en) * | 2022-07-08 | 2022-10-18 | 天津大学 | Collection system suitable for air-borne pollen slide microscopic image |
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