CN220201947U - Single cell screening, arranging, collecting and detecting device - Google Patents
Single cell screening, arranging, collecting and detecting device Download PDFInfo
- Publication number
- CN220201947U CN220201947U CN202321569328.6U CN202321569328U CN220201947U CN 220201947 U CN220201947 U CN 220201947U CN 202321569328 U CN202321569328 U CN 202321569328U CN 220201947 U CN220201947 U CN 220201947U
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- Prior art keywords
- liquid
- collecting box
- wall
- fixedly connected
- single cell
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- 238000012216 screening Methods 0.000 title claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 62
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 238000002347 injection Methods 0.000 claims abstract description 10
- 239000007924 injection Substances 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims description 5
- 238000003556 assay Methods 0.000 claims 6
- 238000004113 cell culture Methods 0.000 abstract description 23
- 239000006285 cell suspension Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000002699 waste material Substances 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Abstract
The utility model discloses a single-cell screening, arranging, collecting and detecting device which comprises a collecting box, wherein the lower surface of the collecting box is fixedly connected with a bottom bracket, the inner bottom wall of the collecting box is fixedly connected with a liquid conveying pipe, the inner wall of the liquid conveying pipe is rotatably connected with a liquid guide pipe, and the upper surface of the liquid guide pipe is fixedly connected with a gear plate. This single cell screening is arranged and is collected detection device, through collecting the box, send the liquid pipe, the catheter, the toothed disc, lug and the setting of culture dish, utilize the syringe to inject cell culture liquid through the injection mouth and get into conveying hose, the check valve in the conveying hose can prevent cell culture liquid refluence, cell culture liquid in the conveying hose gets into after sending the liquid pipe rethread catheter to the play liquid hole entering chute of lug upper surface, through the setting of chute, cell culture liquid can be evenly carried to in the culture dish, no longer need the manual work to carry out independent interpolation cell culture liquid to a plurality of culture dishes, reduce the manpower extravagant.
Description
Technical Field
The utility model relates to the technical field of cell collection devices, in particular to a single cell screening, arranging, collecting and detecting device.
Background
The single cell suspension is the basis for in-vitro cell culture and flow cell analysis, and the traditional method for collecting the single cell suspension is to filter tissue cells through a cell sieve at present, so that the single cell suspension is obtained, but the cell sieve has the defects that the filtering area is too small, the single cell suspension is not suitable for treating a large amount of tissue cell mixed solution, and the effective components of a single cell suspension sample are easily inactivated in the collecting process because an operator is difficult to control the separation time.
Through searching, chinese patent publication (bulletin) No. CN214881470U discloses a cell collection device, and this patent passes through the setting of boiler mechanism, and a large amount of treatment tissue cell mixed liquor, and the single cell suspension that obtains simultaneously is because low temperature environment, activity is improved, although this patent can guarantee single cell suspension's activity, but at present after collecting single cell, need add cell culture to guarantee cell's activity, and current single cell collection detection device mostly need the manual work to add cell culture liquid, and when detecting the single cell of collecting, need take out single cell earlier and detect easily, put into the collection device again after detecting, the process is complicated, influence detection effect, therefore it is very necessary to design a single cell screening arrangement collection detection device.
Disclosure of Invention
The utility model mainly aims to provide a single cell screening, arranging, collecting and detecting device which can effectively solve the problems in the background technology.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:
the single-cell screening, arranging, collecting and detecting device comprises a collecting box, wherein the lower surface of the collecting box is fixedly connected with a bottom bracket, the inner bottom wall of the collecting box is fixedly connected with a liquid conveying pipe, the inner wall of the liquid conveying pipe is rotationally connected with a liquid guide pipe, the upper surface of the liquid guide pipe is fixedly connected with a gear disc, the inner wall of the gear disc is provided with a protruding block, one side of the outer surface of the protruding block is provided with a liquid outlet hole, and the liquid outlet hole is communicated with the liquid guide pipe; the chute has been seted up to surface one side of lug, the inner wall fixedly connected with culture dish of gear plate, the chute switches on with the culture dish mutually, surface one side fixedly connected with conveying hose of liquid feed pipe, conveying hose runs through inner wall one side of collecting box and extends to surface one side of collecting box, conveying hose's inner wall is provided with the check valve, the injection mouth has been seted up to conveying hose's one end.
In order to enable the collection box to be closed, as the single-cell screening arrangement collection detection device, a box cover is connected to the upper surface of the collection box in a threaded manner.
In order to seal the observation hole, the single cell screening, arranging, collecting and detecting device is characterized in that the observation hole is formed in the upper surface of the box cover, and the inner wall of the observation hole is connected with the sealing cover in a threaded manner.
In order to facilitate prevention of a microscope, as a single cell screening arrangement collection detection device, a microscope placing table is fixedly connected to the upper surface of the box cover.
In order to enable the gear disc to rotate, as the single-cell screening, arranging, collecting and detecting device, disclosed by the utility model, the inner wall of the collecting box is rotatably connected with the worm, the worm penetrates through one side of the inner wall of the collecting box and extends to one side of the outer surface of the collecting box, and the worm is in meshed connection with the gear disc.
In order to facilitate the rotation of the worm, the single-cell screening, arranging, collecting and detecting device is used as a single-cell screening, arranging, collecting and detecting device, and two ends of the worm are fixedly connected with rotating shafts.
In order to enable ventilation, as a single cell screening arrangement collection detection device of the present utility model, a ventilation hole is formed on one side of the outer surface of the collection box.
Compared with the prior art, the utility model has the following beneficial effects:
1. according to the utility model, through the arrangement of the collection box, the liquid conveying pipe, the liquid guide pipe, the gear disc, the lug and the culture dishes, the cell culture liquid is injected into the conveying hose through the injection port by using the injector, the check valve in the conveying hose can prevent the cell culture liquid from flowing backwards, the cell culture liquid in the conveying hose enters the liquid conveying pipe and then enters the chute through the liquid guide pipe to the liquid outlet hole on the upper surface of the lug, and through the arrangement of the chute, the cell culture liquid can be uniformly conveyed into the culture dishes, the independent addition of the cell culture liquid to a plurality of culture dishes by manpower is not needed, and the manpower waste is reduced.
2. According to the utility model, through the arrangement of the worm, the rotating shaft, the box cover, the sealing cover, the microscope placing table and the gear disc, when the single cell in the collection box is needed to be detected in specific use, the microscope is placed on the microscope placing table, the sealing cover is screwed off, the microscope lens is aligned to the observation hole, the rotating shaft drives the worm to rotate, the worm and the gear disc perform meshing motion so that the gear disc can rotate, the culture dishes on the inner wall of the gear disc can rotate to the observation hole one by one, the single cell in the culture dishes can be detected by using the microscope, the process of taking the single cell out of the culture dishes to be detected singly and putting the single cell back into the culture dishes after detection is avoided, and the efficiency of detecting the single cell is improved.
Drawings
FIG. 1 is a schematic elevational view of the present utility model;
FIG. 2 is a schematic view of the worm structure of the present utility model;
FIG. 3 is a schematic view of a liquid feeding tube according to the present utility model;
FIG. 4 is a schematic view of a catheter according to the present utility model;
fig. 5 is a schematic view of the structure of the injection port of the present utility model.
In the figure: 1. a collection box; 2. a bottom support; 3. a liquid feeding pipe; 4. a catheter; 5. a gear plate; 6. a bump; 7. a chute; 8. a culture dish; 9. a conveying hose; 10. an injection port; 11. a box cover; 12. a cover; 13. a microscope stand; 14. a worm; 15. a rotating shaft; 16. and (3) a vent hole.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in fig. 1-5, a single cell screening, arranging, collecting and detecting device comprises a collecting box 1, wherein the lower surface of the collecting box 1 is fixedly connected with a bottom support 2, the inner bottom wall of the collecting box 1 is fixedly connected with a liquid feeding pipe 3, the inner wall of the liquid feeding pipe 3 is rotationally connected with a liquid guide pipe 4, the upper surface of the liquid guide pipe 4 is fixedly connected with a gear disc 5, the inner wall of the gear disc 5 is provided with a convex block 6, one side of the outer surface of the convex block 6 is provided with a liquid outlet hole, and the liquid outlet hole is communicated with the liquid guide pipe 4;
in this embodiment, the chute 7 has been seted up on surface one side of lug 6, the inner wall fixedly connected with culture dish 8 of toothed disc 5, chute 7 switches on with culture dish 8 mutually, the surface one side fixedly connected with conveying hose 9 of liquid feed pipe 3, conveying hose 9 runs through the inner wall one side of collecting box 1 and extends to surface one side of collecting box 1, the inner wall of conveying hose 9 is provided with the check valve, injection port 10 has been seted up to one end of conveying hose 9.
When the cell culture liquid injection device is specifically used, the cell culture liquid is injected into the conveying hose 9 through the injection port 10 by using the injector, the cell culture liquid can be prevented from flowing backwards through the check valve in the conveying hose 9, the cell culture liquid in the conveying hose 9 enters the liquid conveying pipe 3 and then passes through the liquid guide pipe 4 to the liquid outlet hole on the upper surface of the convex block 6 to enter the chute 7, the cell culture liquid can be uniformly conveyed into the culture dishes 8 through the arrangement of the chute 7, the cell culture liquid does not need to be independently added into the plurality of culture dishes 8 manually, and the manpower waste is reduced.
In this embodiment, the upper surface of the collecting box 1 is screw-coupled with a box cover 11.
In specific use, the box cover 11 is connected to the upper surface of the collecting box 1 through threads, so that the collecting box 1 can be closed.
In this embodiment, an observation hole is formed in the upper surface of the box cover 11, and a cover 12 is screwed to the inner wall of the observation hole.
In specific use, the observation hole can be exposed after the cover 12 is screwed off, so that single cells in the culture dish 8 can be conveniently detected through the observation hole by using a microscope.
In this embodiment, a microscope stand 13 is fixedly connected to the upper surface of the cover 11.
In particular use, the microscope placement table 13 is provided to facilitate smooth placement of the microscope for inspection.
In this embodiment, the inner wall of the collecting box 1 is rotatably connected with a worm 14, the worm 14 penetrates through one side of the inner wall of the collecting box 1 and extends to one side of the outer surface of the collecting box 1, and the worm 14 is in meshed connection with the gear disc 5.
When the device is specifically used, the worm 14 can be meshed with the gear plate 5 during rotation, so that the gear plate 5 can be driven to rotate during rotation of the worm 14, and the culture dishes 8 in the gear plate 5 can be rotated to the observation holes one by one.
In this embodiment, the two ends of the worm 14 are fixedly connected with the rotating shaft 15.
In particular use, the shaft 15 is arranged to facilitate rotation of the worm 14 by rotating the shaft 15.
In this embodiment, the collecting box 1 is provided with a vent hole 16 on one side of the outer surface.
In specific use, the vent hole 16 can have the effect of ventilating the collection box 1, and the collection box 1 is placed to be closed, so that the oxygen is insufficient, and the single cell activity is poor.
Working principle: in use, the cell culture solution is injected into the conveying hose 9 through the injection port 10 by using the injector, the check valve in the conveying hose 9 can prevent the cell culture solution from flowing backwards, the cell culture solution in the conveying hose 9 enters the liquid conveying pipe 3 and then flows into the chute 7 from the liquid outlet hole on the upper surface of the lug 6 through the liquid guide pipe 4, the cell culture solution can be uniformly conveyed into the culture dishes 8 through the arrangement of the chute 7, the cell culture solution does not need to be independently added into the culture dishes 8 manually, the manpower waste is reduced, when single cells in the collection box 1 need to be detected, the microscope is placed on the microscope placing table 13, the sealing cover 12 is screwed off, the microscope lens is aligned with the observation hole, the rotating shaft 15 drives the worm 14 to rotate, the worm 14 and the gear disc 5 are meshed, so that the gear disc 5 can rotate, the culture dishes 8 on the inner wall of the gear disc 5 can rotate to the observation hole one by one, the single cells in the culture dishes 8 can be detected by using the microscope, the single cells in the culture dishes 8 do not need to be independently taken out to be detected, the single cells in the culture dishes 8 after the detection are detected, and the single cell returning process is improved.
The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (7)
1. The utility model provides a single cell screening arrangement collection detection device, includes collection box (1), its characterized in that: the liquid collecting box is characterized in that the bottom surface of the collecting box (1) is fixedly connected with a bottom support (2), the inner bottom wall of the collecting box (1) is fixedly connected with a liquid conveying pipe (3), the inner wall of the liquid conveying pipe (3) is rotationally connected with a liquid guide pipe (4), the upper surface of the liquid guide pipe (4) is fixedly connected with a gear disc (5), the inner wall of the gear disc (5) is provided with a lug (6), one side of the outer surface of the lug (6) is provided with a liquid outlet hole, and the liquid outlet hole is communicated with the liquid guide pipe (4);
chute (7) have been seted up on surface one side of lug (6), inner wall fixedly connected with culture dish (8) of gear dish (5), chute (7) are linked together with culture dish (8), surface one side fixedly connected with conveying hose (9) of liquid delivery pipe (3), conveying hose (9) run through inner wall one side of collecting box (1) and extend to surface one side of collecting box (1), the inner wall of conveying hose (9) is provided with the check valve, injection mouth (10) have been seted up to one end of conveying hose (9).
2. The single cell screening arrangement collection assay of claim 1 wherein: the upper surface of the collecting box (1) is connected with a box cover (11) in a threaded manner.
3. The single cell screening arrangement collection assay of claim 2 wherein: an observation hole is formed in the upper surface of the box cover (11), and a sealing cover (12) is connected to the inner wall of the observation hole in a threaded mode.
4. The single cell screening arrangement collection assay of claim 2 wherein: the upper surface of the box cover (11) is fixedly connected with a microscope placing table (13).
5. The single cell screening arrangement collection assay of claim 1 wherein: the inner wall of the collecting box (1) is rotationally connected with a worm (14), the worm (14) penetrates through one side of the inner wall of the collecting box (1) and extends to one side of the outer surface of the collecting box (1), and the worm (14) is in meshed connection with the gear disc (5).
6. The single cell screening array collection assay of claim 5 wherein: both ends of the worm (14) are fixedly connected with rotating shafts (15).
7. The single cell screening arrangement collection assay of claim 1 wherein: a vent hole (16) is formed in one side of the outer surface of the collecting box (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321569328.6U CN220201947U (en) | 2023-06-20 | 2023-06-20 | Single cell screening, arranging, collecting and detecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321569328.6U CN220201947U (en) | 2023-06-20 | 2023-06-20 | Single cell screening, arranging, collecting and detecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220201947U true CN220201947U (en) | 2023-12-19 |
Family
ID=89144993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321569328.6U Active CN220201947U (en) | 2023-06-20 | 2023-06-20 | Single cell screening, arranging, collecting and detecting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220201947U (en) |
-
2023
- 2023-06-20 CN CN202321569328.6U patent/CN220201947U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant |