CN216274166U

CN216274166U - Autologous pulp stem cell extraction device

Info

Publication number: CN216274166U
Application number: CN202023333001.8U
Authority: CN
Inventors: 赵辉; 高萧芳; 孙宪凯
Original assignee: Shenzhen Source Gene Technology Co ltd; Yuanchuanggene Chongqing Cell Application Technology Research Institute Co ltd; Zhengzhou Bain Biotechnology Co ltd; Zhengzhou Revo Gene Industry Co ltd
Current assignee: Shenzhen Source Gene Technology Co ltd; Yuanchuanggene Chongqing Cell Application Technology Research Institute Co ltd; Zhengzhou Bain Biotechnology Co ltd; Zhengzhou Revo Gene Industry Co ltd
Priority date: 2020-12-29
Filing date: 2020-12-29
Publication date: 2022-04-12
Anticipated expiration: 2030-12-29

Abstract

The utility model relates to an autologous dental pulp stem cell extraction device, which comprises a support frame, wherein a stirring bin is fixedly connected to an upright post of the support frame, the top of the stirring bin is provided with a sample inlet and a decomposition enzyme port, the bottom of the stirring bin is provided with a discharge port, and the discharge port is connected with a separation bottle; the separation bottle is connected with the base of the support frame through a first gear, a motor is fixed on the base, and a second gear fixedly connected with the motor is meshed with the first gear. The nozzle of the centrifugal tube is prevented from being exposed in the air in the centrifugal process, so that the pollution of cell sap is prevented, and the quality of dental pulp stem cells is ensured.

Description

Autologous pulp stem cell extraction device

Technical Field

The utility model relates to the technical field of stem cell extraction, in particular to an autologous dental pulp stem cell extraction device.

Background

The stem cell is a kind of multipotential cell with self-replicating ability, under a certain condition, the stem cell can be differentiated into various functional cells, and the dental pulp stem cell is a kind of stem cell extracted from human tooth tissue.

In the extraction of the dental pulp stem cells, the fallen teeth can be wrapped by sterilized gauze, the teeth are extruded and crushed to leak out of the dental pulp cavity, the dental pulp tissues are collected into a centrifuge tube after being taken out, a proper amount of collagenase and neutral protease are respectively added to form a mixed culture solution, the centrifuge tube is rotated to decompose the dental pulp stem cells by using the culture solution, so that the dental pulp stem cells are extracted, however, generally, when the collagenase and the neutral protease are added, the mouth of the centrifuge tube can be directly exposed in the air, and the pollution or damage of the dental pulp stem cells is easily caused.

Disclosure of Invention

In order to solve the above problems, an object of the present invention is to provide an autologous dental pulp stem cell extraction device that avoids contamination or damage during the extraction of dental pulp stem cells.

The technical problem to be solved by the utility model is realized by adopting the following technical scheme:

the utility model provides an autologous pulp stem cell extraction element which characterized in that: the device comprises a support frame, wherein a stirring bin is fixedly connected to an upright post of the support frame, the top of the stirring bin is provided with a sample inlet and a decomposition enzyme port, the bottom of the stirring bin is provided with a discharge port, and the discharge port is connected with a separation bottle; the separation bottle is connected with the base of the support frame through a first gear, a motor is fixed on the upright post, and a second gear fixedly connected with the motor is meshed with the first gear.

Further, the middle part of the stirring bin is provided with a stirring part which is fixedly connected with a stirring motor, and the stirring part is driven by the stirring motor to rotate to complete stirring.

Further, the stirring bin is connected with the upright post through a clamping device, and the clamping device can move up and down on the upright post.

Furthermore, the discharge hole is divided into two layers to form an annular sandwich structure.

Further, the discharge port is connected with the separation bottle through a bearing, and the bearing is fixed on the outer layer between the discharge port sandwich structures; the bottle mouth of the separation bottle is movably arranged in the interlayer structure, so that the outer side of the bottle mouth of the separation bottle is connected with the bearing, and the inner side of the bottle mouth is movably connected with the inner layer of the discharge port interlayer structure.

Furthermore, a one-way ball valve is arranged on the discharge hole.

Furthermore, the decomposition enzyme port is connected with a decomposition enzyme tank, scales are marked on the tank wall of the decomposition enzyme tank, and a throttle valve is arranged on the decomposition enzyme port.

Furthermore, a discharge opening is formed in the center of the bottom of the separation bottle, a through hole matched with the discharge opening is formed in the base of the support frame, a discharge pipe penetrating through the through hole is arranged on the discharge opening, and a stop valve is arranged on the discharge pipe.

The utility model discloses an autologous dental pulp stem cell extraction device, which has the beneficial effects that: the stirring bin is connected with the separating flask, and the centrifuge flask is connected with the stirring bin bearing, does not influence the stirring bin when the separating flask is rotatory, has also avoided simultaneously exposing the mouth of pipe of centrifuging tube in the air at centrifugal process, prevents to cause the pollution of cell liquid, guarantees the quality of pulp stem cell. The mixing part is arranged, so that the dental pulp and the decomposition enzyme are fully mixed by the mixing of the mixing part, and the quality of separation and purification is improved.

Drawings

The utility model is described in detail below with reference to examples and figures, in which:

fig. 1 is a schematic structural diagram of an autologous dental pulp stem cell extraction device 1 according to an embodiment of the present invention.

Fig. 2 is a schematic structural diagram of an autologous dental pulp stem cell extraction device in accordance with embodiment 2 of the present invention.

In the attached drawing, 1 is the support frame, 2 is the stand, 3 is the base, 4 is clamping device, 5 is the introduction port, 6 is the resolvase mouth, 7 is the resolvase jar, 8 is the stirring storehouse, 9 is the stirring portion, 10 is agitator motor, 11 is the discharge gate, 12 is the check ball valve, 13 is the bearing, 14 is the knockout drum, 15 is first gear, 16 is the second gear, 17 is the motor, 18 is the discharge tube, 19 is the stop valve, 20 is the discharge opening, 21 is the choke valve.

Detailed Description

The embodiments of the utility model are further illustrated in the following figures:

example 1:

referring to the attached figure 1, the autologous dental pulp stem cell extraction device comprises a support frame 1, wherein a stirring bin 8 is fixedly connected to a stand column 2 of the support frame 1, a sample inlet 5 and a decomposition enzyme port 6 are arranged at the top of the stirring bin 8, a discharge port 11 is arranged at the bottom of the stirring bin 8, and the discharge port 11 is connected with a separation bottle 14; the separation bottle 14 is connected with the base 3 of the support frame 1 through a first gear 15, a motor 17 is fixed on the upright post 2, and a second gear 16 fixedly connected with the motor 17 is meshed with the first gear 15. The stirring bin 8 is connected with the separation bottle 14, so that the pollution caused in the separation and purification process is prevented, and the quality of dental pulp stem cells is prevented from being influenced. The pulp solution and the decomposing enzyme are added from the top of the stirring bin 8 and are stirred and mixed uniformly, so that the quality of separation and purification is improved.

In order to mix the pulp solution and the decomposing enzyme more uniformly, the middle part of the stirring bin 8 is provided with a stirring part 9, the stirring part 9 is fixedly connected with a stirring motor 10, and the stirring part 9 is driven by the stirring motor 10 to rotate to complete stirring. Stirring storehouse 8 passes through clamping device 4 and connects stand 2, and clamping device 4 can reciprocate on stand 2. The stirring part 9 penetrates through the wall of the stirring bin 8, so that the stirring motor 10 is arranged outside the stirring bin 8.

In order to reduce the friction when the separation bottle 14 rotates and to avoid the influence on the stirring chamber 8 when rotating. The discharge port 11 is formed in two layers to form an annular sandwich structure. The discharge port 11 is connected with the separation bottle 14 through a bearing 13, and the bearing 13 is fixed on the outer layer between the sandwich structures of the discharge port 11; the bottle mouth of the separation bottle 14 is movably arranged in the sandwich structure, so that the outer side of the bottle mouth of the separation bottle 14 is connected with the bearing 13, and the inner side of the bottle mouth is movably connected with the inner layer of the sandwich structure of the discharge hole 11. The outer side of the bearing 13 is fixed on the outer layer of the sandwich structure of the discharge port 11, and the inner side is connected with the outer side of the bottle mouth of the separation bottle 14. Preferably, a groove is formed in the inner side wall of the bearing 13, and the outer side of the bottle mouth of the separation bottle 14 is connected with the bearing 13 through a ring-shaped member provided with a protrusion corresponding to the groove, so that the protrusion is just arranged in the groove.

The discharge port 11 is provided with a one-way ball valve 12.

The decomposing enzyme port 6 is connected with a decomposing enzyme tank 7, scales are marked on the tank wall of the decomposing enzyme tank 7, and a throttle valve 21 is arranged on the decomposing enzyme port 6. The amount of added lytic enzyme is controlled by the scale set.

Example 2:

referring to fig. 2, the difference from embodiment 1 is that a discharge opening 20 is provided at the bottom center of the separation bottle 14, a through hole matching with the discharge opening 20 is provided on the base 3 of the support frame 1, a discharge pipe 18 penetrating through the through hole is provided on the discharge opening 20, and a stop valve 19 is provided on the discharge pipe 18. After centrifugation is complete, the pouring of the separated liquid is completed without removing the separation flask 14.

In the implementation process of the utility model, after the device is installed, firstly pulp solution is added into the stirring bin 8 from the sample inlet 5, the throttle valve 21 is opened, the required amount of the decomposition enzyme is added into the stirring bin 8 from the decomposition enzyme port 6, the stirring motor 10 is started in the process of adding the pulp solution and the decomposition enzyme, and the mixed solution is stirred by the stirring part 9, so that the pulp and the decomposition enzyme are fully mixed. And (3) closing the throttle valve 21 after adding the decomposition enzyme, opening the one-way ball valve 12 after uniform mixing to enable the mixed solution to flow into the separation bottle 14 from the discharge hole 11, opening the motor 17, driving the first gear 15 and the separation bottle 14 to rotate by the second gear 16 under the driving of the motor 17 to separate dental pulp in the separation bottle 14, and separating dental pulp stem cells from the mixed solution after centrifugal treatment to separate the dental pulp stem cells.

According to the utility model, the clamping device 4 can be moved to enable the clamping device 4 to drive the stirring bin 8 to move upwards, so that the stirring bin 8 is separated from the separation bottle 14, the separation bottle 14 is taken down, and stem cells are sucked out by a dropper; the separation can also be performed after pouring out by opening the stop valve 19 at the bottom of the separation bottle 14 without moving the stirring bin 8.

The above embodiments are only preferred embodiments of the present invention, and the description is only for the purpose of assisting understanding of the method and the core idea of the present invention, and is not intended to limit the present invention, and any modifications, equivalents and improvements made by those skilled in the art within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides an autologous pulp stem cell extraction element which characterized in that: the device comprises a support frame, wherein a stirring bin is fixedly connected to an upright post of the support frame, the top of the stirring bin is provided with a sample inlet and a decomposition enzyme port, the bottom of the stirring bin is provided with a discharge port, and the discharge port is connected with a separation bottle; the separation bottle is connected with the base of the support frame through a first gear, a motor is fixed on the upright post, and a second gear fixedly connected with the motor is meshed with the first gear.

2. The extraction device of autologous dental pulp stem cells of claim 1, wherein: stirring portion is provided with in the middle part of the stirring storehouse, stirring portion fixed connection agitator motor to the rotatory stirring of accomplishing under agitator motor's the drive.

3. The extraction device of autologous dental pulp stem cells of claim 1, wherein: the stirring bin is connected with the upright post through a clamping device, and the clamping device can move up and down on the upright post.

4. The extraction device of autologous dental pulp stem cells of claim 1, wherein: the discharge port is divided into two layers to form an annular sandwich structure.

5. The apparatus for extracting autologous dental pulp stem cells according to claim 4, wherein: the discharge port is connected with the separation bottle through a bearing, and the bearing is fixed on the outer layer between the discharge port sandwich structures; the bottle mouth of the separation bottle is movably arranged in the interlayer structure, so that the outer side of the bottle mouth of the separation bottle is connected with the bearing, and the inner side of the bottle mouth is movably connected with the inner layer of the discharge port interlayer structure.

6. The extraction device of autologous dental pulp stem cells of claim 1, wherein: and the discharge port is provided with a one-way ball valve.

7. The extraction device of autologous dental pulp stem cells of claim 1, wherein: the decomposition enzyme port is connected with a decomposition enzyme tank, scales are marked on the tank wall of the decomposition enzyme tank, and a throttle valve is arranged on the decomposition enzyme port.

8. The extraction device of autologous dental pulp stem cells of claim 1, wherein: the bottom center of separation bottle is provided with the discharge opening, be provided with on the base of support frame with discharge opening assorted through-hole, be provided with the discharge tube that runs through the through-hole on the discharge opening, be provided with the stop valve on the discharge tube.