CN209989380U - Stem cell extraction equipment based on dental pulp - Google Patents
Stem cell extraction equipment based on dental pulp Download PDFInfo
- Publication number
- CN209989380U CN209989380U CN201920071545.XU CN201920071545U CN209989380U CN 209989380 U CN209989380 U CN 209989380U CN 201920071545 U CN201920071545 U CN 201920071545U CN 209989380 U CN209989380 U CN 209989380U
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- Prior art keywords
- stem cell
- cell extraction
- fixedly connected
- fixed connection
- extraction apparatus
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 41
- 238000000605 extraction Methods 0.000 title claims abstract description 25
- 210000003074 dental pulp Anatomy 0.000 title claims abstract description 15
- 238000003756 stirring Methods 0.000 claims abstract description 24
- 238000002156 mixing Methods 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims description 9
- 230000001502 supplementing effect Effects 0.000 claims description 7
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 claims description 6
- 239000013589 supplement Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 15
- 108090000790 Enzymes Proteins 0.000 abstract description 9
- 102000004190 Enzymes Human genes 0.000 abstract description 9
- 238000000354 decomposition reaction Methods 0.000 abstract description 4
- 239000011259 mixed solution Substances 0.000 abstract description 4
- 238000000926 separation method Methods 0.000 abstract description 4
- 238000005119 centrifugation Methods 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 description 8
- 238000001802 infusion Methods 0.000 description 7
- 238000004321 preservation Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
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Abstract
The utility model relates to a stem cell draws technical field, discloses a stem cell draws equipment based on dental pulp, comprising a base plate, bottom plate upper surface left side fixed connection stand, stand top fixed connection stirring storehouse, stand lower part fixed connection crossbeam, the crossbeam middle part is rotated and is connected the pivot, fixed surface connects second driving motor under the crossbeam, the first bevel gear of second driving motor output shaft fixed connection. The utility model discloses can be used for among the stem cell extraction element, constitute mixing mechanism through setting up (mixing) shaft and puddler, make pulp and decomposition enzyme solution intensive mixing through this mechanism to set up pivot and centrifugal bottle and constitute centrifugal mechanism, thereby make stem cell and mixed solution separation, make things convenient for the extraction of later stage stem cell, whole device easy operation, a device has accomplished mix and two works of centrifugation, and convenient to use has improved operating personnel's work efficiency, suitable using widely.
Description
Technical Field
The utility model relates to a stem cell draws technical field, specifically is a stem cell draws equipment based on dental pulp.
Background
Stem cells are primitive cells having self-renewal and multipotential differentiation potential, are cells of origin of the body, can be differentiated into one or more cells constituting tissues or organs of the human body under specific conditions, and are widely used in basic medical research. Self-renewal refers to the ability of stem cells to maintain the size of stem cell populations through cell division; the multipotential differentiation means that stem cells have plasticity and can be differentiated into different tissue cells under different environments so as to form various complex tissues and organs of an organism.
Chinese patent (publication number: CN 208136244U) discloses an adipose-derived stem cell extraction device, which comprises a treatment bag and a preservation bag, wherein the treatment bag and the preservation bag are both sealed bags, the treatment bag and the preservation bag are communicated with each other through a connecting pipe, and a pipe clamp is arranged in the middle of the connecting pipe. A first lead-in pipe and a second lead-in pipe are arranged above the treatment bag, and a fat stem cell collecting pipe and a waste liquid pipe are arranged below the treatment bag. The first inlet pipe and the second inlet pipe are provided with a sealing cover at the inlet, the waste liquid pipe and the adipose-derived stem cell collecting pipe are respectively provided with a pipe clamp, the preservation bag is provided with a third inlet pipe, the third inlet pipe is provided with a sealing cover at the inlet, the device is only provided with a preservation container, but is not provided with a mixing and centrifugal separation device, and still needs to be placed in other equipment for operation, so that the whole extraction work is complex, and the work efficiency is not high.
Disclosure of Invention
The utility model provides a stem cell draws equipment based on dental pulp has solved the problem of proposing among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides a stem cell draws equipment based on dental pulp, includes the bottom plate, bottom plate upper surface left side fixed connection stand, stand top fixed connection stirring storehouse, stand lower part fixed connection crossbeam, crossbeam middle part are rotated and are connected the pivot, fixed surface connects second driving motor under the crossbeam, the first bevel gear of second driving motor output shaft fixed connection, first bevel gear meshing connection second bevel gear, second bevel gear fixed connection pivot lower part, the articulated anchor clamps in pivot upper portion, anchor clamps fixed connection centrifuge bottle, the first driving motor of stand right side fixed connection, the first band pulley of first driving motor output shaft fixed connection, first band pulley pass through the belt and connect the second band pulley, second band pulley fixed connection (mixing) shaft left side, (mixing) shaft middle part fixed connection puddler, stirring storehouse lower part is equipped with the discharge opening.
As a preferred technical scheme of the utility model, anchor clamps include fixed grip block, the articulated activity grip block in fixed grip block left side.
As an optimal technical scheme of the utility model, activity grip block sliding connection bolt, bolt screw thread part coupling nut.
As an optimized technical scheme of the utility model, discharge opening left side wall upper portion articulates baffle, baffle lower part sliding connection slider.
As a preferred technical scheme of the utility model, discharge opening left side wall middle part threaded connection lead screw, the articulated slider of lead screw right-hand member.
As an optimal technical scheme of the utility model, stirring storehouse right side wall fixed connection fluid infusion jar, fluid infusion jar lower part is equipped with the choke valve.
As an optimized technical proposal of the utility model, the inside of the fluid infusion tank is provided with a solution of the decomposition enzyme.
As an optimized technical scheme of the utility model, the external scale that is equipped with of fluid infusion jar.
The utility model discloses have following useful part:
the utility model discloses can be used for among the stem cell extraction element, constitute mixing mechanism through setting up (mixing) shaft and puddler, make pulp and decomposition enzyme solution intensive mixing through this mechanism to set up pivot and centrifugal bottle and constitute centrifugal mechanism, thereby make stem cell and mixed solution separation, make things convenient for the extraction of later stage stem cell, whole device easy operation, a device has accomplished mix and two works of centrifugation, and convenient to use has improved operating personnel's work efficiency, suitable using widely.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
Fig. 1 is a schematic structural view of a stem cell extraction apparatus based on dental pulp.
Fig. 2 is a cross-sectional view a-a of a dental pulp-based stem cell extraction apparatus.
Fig. 3 is a partially enlarged schematic view of B in a dental pulp-based stem cell extraction apparatus.
Fig. 4 is a partially enlarged schematic view of C in a dental pulp-based stem cell extraction apparatus.
In the figure: 1. a base plate; 2. a column; 3. a cross beam; 4. a rotating shaft; 5. centrifuging the bottle; 6. a clamp; 7. a first drive motor; 8. a first pulley; 9. a belt; 10. a second pulley; 11. a stirring shaft; 12. a stirring bin; 13. a liquid replenishing tank; 14. a throttle valve; 15. a stirring rod; 16. a discharge opening; 17. a second bevel gear; 18. a first bevel gear; 19. a second drive motor; 20. fixing the clamping plate; 21. a movable clamping plate; 22. a bolt; 23. a nut; 24. a screw rod; 25. a baffle plate; 26. a slider; 27. a graduated scale; 28. and (3) decomposing the enzyme solution.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example one
Referring to fig. 1 and 2, a stem cell extraction device based on dental pulp comprises a bottom plate 1, wherein an upright post 2 is fixedly connected to the left side of the upper surface of the bottom plate 1, a stirring bin 12 is fixedly connected to the top of the upright post 2, a cross beam 3 is fixedly connected to the lower portion of the upright post 2, a rotating shaft 4 is rotatably connected to the middle portion of the cross beam 3, a second driving motor 19 is fixedly connected to the lower surface of the cross beam 3, an output shaft of the second driving motor 19 is fixedly connected to a first bevel gear 18, the first bevel gear 18 is engaged with a second bevel gear 17, the second bevel gear 17 is fixedly connected to the lower portion of the rotating shaft 4, a clamp 6 is hinged to the upper portion of the rotating shaft 4, the clamp 6 is fixedly connected to a centrifuge bottle 5, the clamp 6 comprises a fixed clamping plate 20, a movable clamping plate 21 is hinged.
Referring to fig. 1 and 3, a first driving motor 7 is fixedly connected to the right side of the upright column 2, an output shaft of the first driving motor 7 is fixedly connected to a first belt pulley 8, the first belt pulley 8 is connected to a second belt pulley 10 through a belt 9, the second belt pulley 10 is fixedly connected to the left side of a stirring shaft 11, a stirring rod 15 is fixedly connected to the middle of the stirring shaft 11, a discharge opening 16 is arranged at the lower portion of the stirring bin 12, a baffle 25 is hinged to the upper portion of the left side wall of the discharge opening 16, a slider 26 is slidably connected to the lower portion of the baffle 25, a lead screw 24 is in threaded connection with the middle portion of the left.
Example two
Referring to fig. 1 and 4, the other contents of the present embodiment are the same as those of the first embodiment, except that: the right side wall of the stirring bin 12 is fixedly connected with a liquid supplementing tank 13, a throttle valve 14 is arranged at the lower part of the liquid supplementing tank 13, a decomposition enzyme solution 28 is arranged inside the liquid supplementing tank 13, and a graduated scale 27 is arranged outside the liquid supplementing tank 13. Since it is necessary to add the lytic enzyme when the stem cells are extracted, it is troublesome to add the lytic enzyme every time of extraction, so the fluid infusion tank 13 is directly arranged on the right side of the stirring bin 12, the lytic enzyme solution 28 is put into the fluid infusion tank 13, the lytic enzyme solution 28 is controlled to enter the stirring bin 12 through the throttle valve 14, and the volume of the added lytic enzyme solution 28 can be controlled according to the graduated scale 27 arranged outside the fluid infusion tank 13.
In the implementation process of the utility model, firstly, the pulp solution is put into the stirring bin 12, the throttle valve 14 is opened, so that the decomposing enzyme solution 28 in the fluid supplementing tank 13 enters the stirring bin 12, the added decomposing enzyme solution 28 is controlled by observing the graduated scale 27, after the adding of the decomposing enzyme solution 28 is finished, the throttle valve 14 is closed, the first driving motor 7 is started, the first driving motor 7 drives the stirring shaft 11 to rotate through belt transmission, so as to drive the stirring rod 15 to rotate, the stirring rod 15 fully mixes the pulp inside the stirring bin 12 with the decomposing enzyme, after the mixing is finished, the screw rod 24 is rotated, the screw rod 24 moves to the left side, so as to drive the baffle 25 to rotate clockwise, the stirred pulp enters the centrifugal bottle 5 through the discharge opening 16, the cover of the centrifugal bottle 5 is covered, and the nut 23 fixes the centrifugal bottle 5 and the screwing clamp 6, at this moment, the second driving motor 19 is started, the second driving motor 19 drives the rotating shaft 4 to rotate through a bevel gear in meshed connection, so that the rotating shaft 4 drives the centrifugal bottle 5 to rotate at a high speed, the dental pulp inside is subjected to centrifugal treatment, stem cells are separated from the mixed solution after the centrifugal treatment, the centrifugal bottle 5 is taken down, and the stem cells are taken out by using a rubber-tipped dropper; the utility model discloses can be used for among the stem cell extraction element, constitute mixing mechanism through setting up (mixing) shaft 11 and puddler 15, make dental pulp and 28 intensive mixings of resolvase solution through this mechanism to set up pivot 4 and centrifuge bottle 5 and constitute centrifugal mechanism, thereby make stem cell and mixed solution separation, make things convenient for the extraction of later stage stem cell, whole device easy operation, a device has accomplished and has mixed and two works of centrifugation, high durability and convenient use, operating personnel's work efficiency has been improved, suitable using widely.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (8)
1. A stem cell extraction device based on dental pulp comprises a bottom plate (1), wherein the left side of the upper surface of the bottom plate (1) is fixedly connected with an upright post (2), the top of the upright post (2) is fixedly connected with a stirring bin (12), the lower part of the upright post (2) is fixedly connected with a cross beam (3), the middle part of the cross beam (3) is rotatably connected with a rotating shaft (4), the stem cell extraction device is characterized in that the lower surface of the cross beam (3) is fixedly connected with a second driving motor (19), an output shaft of the second driving motor (19) is fixedly connected with a first bevel gear (18), the first bevel gear (18) is meshed with a second bevel gear (17), the second bevel gear (17) is fixedly connected with the lower part of the rotating shaft (4), the upper part of the rotating shaft (4) is hinged with a clamp (6), the clamp (6) is fixedly connected with a centrifuge bottle (5), the right side of the upright post, first band pulley (8) pass through belt (9) and connect second band pulley (10), and second band pulley (10) fixed connection (mixing) shaft (11) left side, (mixing) shaft (11) middle part fixed connection puddler (15), stirring storehouse (12) lower part is equipped with discharge opening (16).
2. The endodontic-based stem cell extraction apparatus of claim 1, wherein the clamp (6) includes a fixed clamping plate (20), and a movable clamping plate (21) is hinged to the left side of the fixed clamping plate (20).
3. The endodontic-based stem cell extraction apparatus of claim 2, wherein the movable clamping plate (21) is slidably coupled to a bolt (22), and the threaded portion of the bolt (22) is coupled to a nut (23).
4. The endodontic-based stem cell extraction apparatus of claim 1, wherein the discharge opening (16) has a shutter (25) hinged to an upper portion of a left sidewall, and the shutter (25) is slidably connected to a slider (26) at a lower portion thereof.
5. The endodontic-based stem cell extraction apparatus of claim 1 or 4, wherein a screw (24) is screwed to a middle portion of a left side wall of the discharge opening (16), and a slider (26) is hinged to a right end of the screw (24).
6. The dental pulp-based stem cell extraction apparatus according to claim 1, wherein the right side wall of the stirring bin (12) is fixedly connected with a liquid supplementing tank (13), and a throttle valve (14) is arranged at the lower part of the liquid supplementing tank (13).
7. The endodontic-based stem cell extraction apparatus of claim 6, wherein the solution supplement tank (13) is internally provided with a lytic enzyme solution (28).
8. The endodontic-based stem cell extraction apparatus of claim 7, wherein a scale (27) is provided outside the replenisher tank (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920071545.XU CN209989380U (en) | 2019-01-16 | 2019-01-16 | Stem cell extraction equipment based on dental pulp |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920071545.XU CN209989380U (en) | 2019-01-16 | 2019-01-16 | Stem cell extraction equipment based on dental pulp |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209989380U true CN209989380U (en) | 2020-01-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920071545.XU Expired - Fee Related CN209989380U (en) | 2019-01-16 | 2019-01-16 | Stem cell extraction equipment based on dental pulp |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209989380U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113308370A (en) * | 2021-05-11 | 2021-08-27 | 吕宇彤 | Intelligent extraction equipment and extraction method for stem cells |
-
2019
- 2019-01-16 CN CN201920071545.XU patent/CN209989380U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113308370A (en) * | 2021-05-11 | 2021-08-27 | 吕宇彤 | Intelligent extraction equipment and extraction method for stem cells |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200124 |