CN113308370A - Intelligent extraction equipment and extraction method for stem cells - Google Patents
Intelligent extraction equipment and extraction method for stem cells Download PDFInfo
- Publication number
- CN113308370A CN113308370A CN202110512944.7A CN202110512944A CN113308370A CN 113308370 A CN113308370 A CN 113308370A CN 202110512944 A CN202110512944 A CN 202110512944A CN 113308370 A CN113308370 A CN 113308370A
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- enzyme reaction
- stem cells
- input shaft
- storehouse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 45
- 238000000605 extraction Methods 0.000 title claims abstract description 29
- 238000006911 enzymatic reaction Methods 0.000 claims abstract description 56
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 238000000926 separation method Methods 0.000 claims abstract description 30
- 102000004190 Enzymes Human genes 0.000 claims abstract description 21
- 108090000790 Enzymes Proteins 0.000 claims abstract description 21
- 206010024796 Logorrhoea Diseases 0.000 claims description 20
- 238000013467 fragmentation Methods 0.000 claims description 19
- 238000006062 fragmentation reaction Methods 0.000 claims description 19
- 238000006243 chemical reaction Methods 0.000 claims description 18
- 238000009826 distribution Methods 0.000 claims description 13
- 230000008878 coupling Effects 0.000 claims description 11
- 238000010168 coupling process Methods 0.000 claims description 11
- 238000005859 coupling reaction Methods 0.000 claims description 11
- 238000007599 discharging Methods 0.000 claims description 9
- 238000009434 installation Methods 0.000 claims description 8
- 238000012546 transfer Methods 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims 2
- 230000006978 adaptation Effects 0.000 claims 1
- 210000002826 placenta Anatomy 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 5
- 238000005192 partition Methods 0.000 abstract description 3
- 238000003860 storage Methods 0.000 abstract description 3
- 239000002245 particle Substances 0.000 abstract description 2
- 239000007921 spray Substances 0.000 abstract description 2
- 238000007789 sealing Methods 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 5
- 238000001914 filtration Methods 0.000 description 3
- 210000005059 placental tissue Anatomy 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000011218 segmentation Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000004991 placental stem cell Anatomy 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/05—Means for pre-treatment of biological substances by centrifugation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/09—Means for pre-treatment of biological substances by enzymatic treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention relates to the technical field of stem cell extraction devices, and discloses intelligent stem cell extraction equipment and an extraction method thereof, wherein biological enzymes in a biological enzyme storage tank are discharged into a high-pressure pump through a liquid inlet pipe, are discharged into the liquid inlet pipe and the liquid outlet pipe through the pressurization effect of the high-pressure pump, are discharged into a liquid conveying pipe through the liquid outlet pipe, and are finally sprayed on a placenta through a porous spray head, so that biological enzyme reaction can be realized, placenta particles attached to the inner wall can be cleaned, subsequent operation is facilitated, a discharge partition plate is moved outwards by pulling a semicircular handle outwards, the minced placenta enters an enzyme reaction box, a double-shaft micro-motor is started, the double-shaft micro-motor enables two centrifugal input shafts to rotate, the two centrifugal input shafts respectively drive corresponding eccentric wheels to rotate, so that a vibration pump drives the enzyme reaction box to vibrate under the action of a high-frequency spring, the biological enzyme reaction can be promoted by the vibration of the enzyme reaction box, and the stem cell separation rate is accelerated.
Description
Technical Field
The invention relates to the technical field of stem cell extraction devices, in particular to stem cell intelligent extraction equipment and an extraction method thereof.
Background
Stem cells are a type of pluripotent cells with self-replication ability, which can be differentiated into various functional cells under certain conditions, and are classified into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and are classified into three types according to the development potential of the stem cells: the stem cell is an insufficiently differentiated and immature cell, has the potential function of regenerating various tissues, organs and human bodies, and is called as a universal cell in the medical field.
Used stem cell all draws from human placenta in the medical treatment, stem cell need be with the placenta mince in the extraction process, biological enzyme reaction and filtration, a series of operations all need operating personnel to change different equipment segmentation and go on, this mode is not only time-consuming and energy-consuming, and stem cell bacterial infection's probability has been increaseed, on the other hand, after carrying out shredding to the placenta, adhere to many placenta granules on the conventional shredder mechanism inner wall easily, neither do not do benefit to the stem cell separation and draw, can breed the follow-up use that the bacterium influences the device when the clearance is incomplete.
When the stem cells react with the biological enzyme, a corresponding reaction rate promoting mechanism is needed, the separation rate of the stem cells is increased, the subsequent separation is convenient, meanwhile, the solid-liquid separation is needed in a centrifugal mode, and the problem that the stem cells are not completely separated from placenta tissues due to the traditional static filtration is avoided.
Therefore, we disclose an intelligent extraction device for stem cells to meet the extraction requirement of placental stem cells.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides intelligent extraction equipment for stem cells, which has the advantages of cleaning the inner wall, accelerating enzyme biological reaction, separating solid and liquid and the like, and solves the problems that the placenta needs to be cut up, the enzyme biological reaction and the filtration need to be carried out in the extraction process of the stem cells, operators need to replace different equipment for carrying out a series of operations in a segmented manner, and the bacterial infection probability of the stem cells is increased.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: an intelligent stem cell extraction device comprises a collection bin, wherein the top of the collection bin is fixedly connected with a separation reaction bin, the top of the separation reaction bin is fixedly connected with a crushing bin, the inner wall of one side of the crushing bin is provided with three rectangular limiting grooves which are uniformly distributed, the top of the crushing bin is provided with a sealing cover, the top of the sealing cover is fixedly connected with an arc-shaped handle, the bottom of the sealing cover is fixedly connected with three rectangular limiting blocks, the positions of the three rectangular limiting blocks correspond to the positions of the three rectangular limiting grooves respectively and are matched with each other, the bottom of the sealing cover is fixedly connected with a crushing motor, the output end of the crushing motor is fixedly connected with a coupler, one end of the coupler is fixedly connected with a crushing input shaft, one side, which is close to each other, of the three rectangular limiting blocks is fixedly connected with the same engagement disc, and the top of the engagement disc is provided with a rotating hole, the one end of fragmentation input shaft runs through change the hole, and extend to outside the one side of changeing the hole, one side fixedly connected with fragmentation sword of fragmentation input shaft, the blown down tank has been seted up to one side inner wall of fragmentation feed bin, sliding connection has ejection of compact baffle in the blown down tank.
Preferably, the one end of ejection of compact baffle extends to the outside of garrulous feed bin, the one end fixedly connected with semicircle handle of ejection of compact baffle, the bottom fixedly connected with discharging pipe of garrulous feed bin, the bottom of discharging pipe extends to in the separation reaction storehouse, the bottom fixedly connected with enzyme reaction case of discharging pipe, enzyme reaction case is located in the separation reaction storehouse.
Preferably, a plurality of separating holes which are uniformly distributed are formed in one side of the enzyme reaction box, a guide column is fixedly connected to the inner wall of the bottom of the enzyme reaction box, a vibration pump is arranged at the top end of the guide column, an installation block is fixedly connected to one side of the vibration pump, the position of the installation block corresponds to that of the guide column, and a high-frequency spring is fixedly sleeved on one side of the guide column.
Preferably, the both ends of high frequency spring respectively with enzyme reaction case with the installation piece is connected, fixedly connected with biax micromotor in the reciprocating pump, the equal fixedly connected with centrifugal input shaft in both ends of biax micromotor, two the equal fixedly connected with eccentric wheel in both ends that the each other keeps away from of centrifugal input shaft, the outside of enzyme reaction case is equipped with the liquid collection frame.
Preferably, the liquid collection frame is close to a plurality of even agreeing with lugs that distribute of one side fixedly connected with of enzyme reaction case, a plurality of even that distribute agree with the position of lug respectively with a plurality of even that distribute the position of separation hole corresponds each other, one side fixedly connected with wide-angle camera of liquid collection frame, fixedly connected with centrifugal motor in the collection storehouse.
Preferably, centrifugal motor's output fixedly connected with shaft coupling, the one end fixedly connected with input shaft of shaft coupling, it has seted up the rotation hole to collect the storehouse top, the position in rotation hole with the position of input shaft is corresponding, the one end of input shaft runs through the rotation hole, and extends to outside one side in rotation hole, the top fixedly connected with connection pad of input shaft, centrifugal motor's outside fixedly connected with annular division board.
Preferably, the top of connection pad with the enzyme reaction case is connected, the enzyme reaction case with the collection storehouse the top is seted up same a plurality of collection holes that distribute evenly, collect the top fixedly connected with biological enzyme bin, high-pressure pump and the controller in storehouse, the top fixedly connected with feed liquor pipe of biological enzyme bin, the other end of feed liquor pipe with the high-pressure pump is connected.
Preferably, one side fixedly connected with transfer line of high-pressure pump, annular spacing groove and a plurality of the even perforation of distribution have been seted up in the outside of garrulous feed bin, annular spacing inslot fixedly connected with shunt tubes, a plurality of even porous shower nozzles of distribution of one side fixedly connected with of shunt tubes, the position of a plurality of even porous shower nozzles of distribution respectively with a plurality of the perforated position corresponds each other, and is a plurality of the one end of porous shower nozzle runs through corresponding respectively the perforation, and extends to in one side of garrulous feed bin.
Preferably, one side fixedly connected with drain pipe of transfer line, the one end of drain pipe with the high-pressure pump is connected, the top fixedly connected with data line of high-pressure pump, the one end of data line with the controller is connected, one side fixedly connected with conduction line of controller, the one end of conduction line extends to in the enzyme reaction box, with wide-angle camera is connected, the controller with the conduction line the data line is electric connection.
(III) advantageous effects
Compared with the prior art, the invention provides intelligent extraction equipment for stem cells, which has the following beneficial effects:
1. this stem cell intelligence draws equipment drives the rotation of fragmentation input shaft through the shaft coupling, and the fragmentation input shaft drives the rotation of fragmentation sword, thereby the rotation of fragmentation sword can be minced placenta.
2. This equipment is drawed to stem cell intelligence, through the feed liquor pipe in with the biological enzyme in the biological enzyme bin arrange the high-pressure pump, through the supercharging of high-pressure pump, and then in arranging biological enzyme into the discharge liquid pipe, in the discharge liquid pipe row gets into the transfer line, spills on the placenta through porous shower nozzle at last to can realize biological enzyme reaction, can clear up the follow-up operation of being convenient for to the adnexed placenta granule of inner wall simultaneously.
3. This equipment is drawed to stem cell intelligence, through outside pulling semicircle handle, make ejection of compact baffle outwards remove, thereby make the placenta after cutting up get into the enzyme reaction incasement, start the little motor of biax, the little motor of biax makes two centrifugal input shafts rotate, two centrifugal input shafts drive corresponding eccentric wheel respectively and rotate, make the reciprocating pump drive enzyme reaction case vibrations under the effect of high frequency spring, thereby can promote biological enzyme reaction through the vibrations of enzyme reaction case, accelerate stem cell separation rate.
4. This equipment is drawed to stem cell intelligence, the output through centrifugal motor passes through the shaft coupling and makes the input shaft rotate, and the input shaft and then makes the connection pad rotate, thereby the connection pad makes the enzyme reaction case rotate, rotates through the enzyme reaction case and makes liquid throw away through separation hole and collection hole and get into in the collection storehouse to make stem cell in the placenta tissue separation more thorough, thereby realized the integration operation, avoid changing different equipment segmentation and go on and increase the infection probability.
5. This stem cell intelligence draws equipment, through the setting of wide angle camera for enzyme reaction box separation hole can be corresponding with the position that agrees with the lug after the stall, corresponds in the back spreads into the controller with the signal through the conducting wire, thereby closes centrifugal motor through the controller.
6. This equipment is drawed to stem cell intelligence through the setting of liquid collection frame, can effectively inject the liquid of throwing away in the enzyme reaction case in collection hole department, on the one hand, can prevent that the liquid of throwing away from spatters the inner wall in separation reaction storehouse, and on the other hand has effectively improved the collection rate of liquid, has reduced the loss of liquid.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic cross-sectional perspective view of the present invention;
FIG. 3 is a schematic cross-sectional perspective view of a material crushing bin according to the present invention;
FIG. 4 is a schematic perspective view of a reducing input shaft according to the present invention;
FIG. 5 is a schematic sectional view of a reaction chamber according to the present invention;
FIG. 6 is a schematic cross-sectional perspective view of a fluid collection frame according to the present invention;
FIG. 7 is a schematic sectional perspective view of an enzyme reaction cassette according to the present invention;
FIG. 8 is a schematic sectional perspective view of a vibration pump according to the present invention;
FIG. 9 is a schematic view of the present invention in a partially cut-away perspective configuration;
fig. 10 is a schematic perspective view of a portion of the present invention.
In the figure: 1. a collection bin; 2. separating the reaction bin; 3. a crushing bin; 4. a rectangular limiting groove; 5. a sealing cover; 6. an arc-shaped handle; 7. a rectangular limiting block; 8. a shredding motor; 9. fragmenting the input shaft; 10. fitting the disc; 11. a shredding knife; 12. a discharge chute; 13. a discharge partition plate; 14. a semicircular handle; 15. a discharge pipe; 16. an enzyme reaction chamber; 17. a separation well; 18. a guide post; 19. vibrating the pump; 20. mounting blocks; 21. a high-frequency spring; 22. a dual-axis micromotor; 23. a centrifugal input shaft; 24. an eccentric wheel; 25. a liquid collection frame; 26. fitting the bump; 27. a wide-angle camera; 28. a centrifugal motor; 29. an annular partition plate; 30. a connecting disc; 31. a collection well; 32. a biological enzyme storage tank; 33. a high pressure pump; 34. a controller; 35. a liquid inlet pipe; 36. a transfusion tube; 37. a shunt tube; 38. a porous showerhead; 39. a liquid outlet pipe; 40. annular spacing groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As described in the background of the invention, the prior art has shortcomings, and in order to solve the above technical problems, the present application proposes an intelligent stem cell extraction device
In a typical embodiment of the present application, as shown in fig. 1-10, an intelligent stem cell extraction device comprises a collection bin 1, a separation reaction bin 2 is fixedly connected to the top of the collection bin 1, a crushing bin 3 is fixedly connected to the top of the separation reaction bin 2, three rectangular limiting grooves 4 uniformly distributed are formed in the inner wall of one side of the crushing bin 3, a sealing cover 5 is arranged on the top of the crushing bin 3, an arc-shaped handle 6 is fixedly connected to the top of the sealing cover 5, three rectangular limiting blocks 7 are fixedly connected to the bottom of the sealing cover 5, the three rectangular limiting blocks 7 correspond to the three rectangular limiting grooves 4 respectively and are matched with each other, a crushing motor 8 is fixedly connected to the bottom of the sealing cover 5, a coupler is fixedly connected to the output end of the crushing motor 8, a crushing input shaft 9 is fixedly connected to one end of the coupler, and the same engagement disc 10 is fixedly connected to the side where the three rectangular limiting blocks 7 are close to each other, the hole that changes has been seted up at the top of agreeing with dish 10, and the one end of fragmentation input shaft 9 runs through the hole that changes, and outside extending to the one side in hole, one side fixedly connected with fragmentation sword 11 of fragmentation input shaft 9, discharge chute 12 has been seted up to one side inner wall in crushed aggregates storehouse 3, and sliding connection has ejection of compact baffle 13 in discharge chute 12.
Further, in above-mentioned scheme, the one end of ejection of compact baffle 13 extends to the outside of garrulous feed bin 3, the one end fixedly connected with semicircle handle 14 of ejection of compact baffle 13, the bottom fixedly connected with discharging pipe 15 in garrulous feed bin 3, the bottom of discharging pipe 15 extends to in the separation reaction storehouse 2, the bottom fixedly connected with enzyme reaction case 16 of discharging pipe 15, enzyme reaction case 16 is located separation reaction storehouse 2, through the setting of semicircle handle 14, the ejection of compact baffle 15 of being convenient for is pulled out.
Furthermore, in the above scheme, a plurality of uniformly distributed separation holes 17 are formed in one side of the enzyme reaction box 16, a guide column 18 is fixedly connected to the inner wall of the bottom of the enzyme reaction box 16, a vibration pump 19 is arranged at the top end of the guide column 18, an installation block 20 is fixedly connected to one side of the vibration pump 19, the position of the installation block 20 corresponds to the position of the guide column 18, a high-frequency spring 21 is fixedly sleeved on one side of the guide column 18, and the vibration of the enzyme reaction box 16 is facilitated through the arrangement of the high-frequency spring 21.
Further, in the above scheme, two ends of the high-frequency spring 21 are respectively connected with the enzyme reaction box 16 and the mounting block 20, the vibration pump 19 is internally and fixedly connected with the double-shaft micro motor 22, two ends of the double-shaft micro motor 22 are both fixedly connected with the centrifugal input shafts 23, two ends of the two centrifugal input shafts 23, which are far away from each other, are both fixedly connected with the eccentric wheels 24, the outer side of the enzyme reaction box 16 is provided with the liquid collection frame 25, and the vibration pump 19 can vibrate through the arrangement of the eccentric wheels 24.
Further, in the above scheme, one side of the liquid collection frame 25 close to the enzyme reaction tank 16 is fixedly connected with a plurality of engaging bumps 26 which are uniformly distributed, the positions of the engaging bumps 26 which are uniformly distributed correspond to the positions of the separating holes 17 which are uniformly distributed, one side of the liquid collection frame 25 is fixedly connected with a wide-angle camera 27, the interior of the collection bin 1 is fixedly connected with a centrifugal motor 28, and the positions of the engaging bumps 26 and the separating holes 17 can be detected through the arrangement of the wide-angle camera 27.
Further, in the above scheme, the output fixedly connected with shaft coupling of centrifugal motor 28, the one end fixedly connected with input shaft of shaft coupling, the rotation hole has been seted up at collection storehouse 1 top, the position in rotation hole is corresponding with the position of input shaft, the one end of input shaft runs through the rotation hole, and outside extending to one side in rotation hole, the top fixedly connected with connection pad 30 of input shaft, centrifugal motor 28's outside fixedly connected with annular division board 29, through the setting of annular division board 29, prevent that liquid from getting into and collecting storehouse 1 in with the contact of centrifugal motor 28.
Further, in the above scheme, the top of connection pad 30 is connected with enzyme reaction box 16, enzyme reaction box 16 and the top of collecting bin 1 are seted up same a plurality of collection holes 31 that distribute evenly, the top fixedly connected with biological enzyme bin 32 of collecting bin 1, high-pressure pump 33 and controller 34, the top fixedly connected with feed liquor pipe 35 of biological enzyme bin 32, the other end of feed liquor pipe 35 is connected with high-pressure pump 33, through the setting of collection hole 31, be convenient for liquid gets into in the collecting bin 1.
Further, in above-mentioned scheme, one side fixedly connected with transfer line 36 of high-pressure pump 33, annular spacing groove 40 and the even perforation of a plurality of distributions have been seted up to the outside in crushed aggregates storehouse 3, fixedly connected with shunt tubes 37 in the annular spacing groove 40, a plurality of even porous shower nozzles 38 of distribution of one side fixedly connected with of shunt tubes 37, the even porous shower nozzle 38's of a plurality of distributions position corresponds with a plurality of fenestrate positions each other respectively, corresponding perforation is run through respectively to the one end of a plurality of porous shower nozzles 38, and extend to in one side of crushed aggregates storehouse 3, through fenestrate setting, be convenient for porous shower nozzle get into in the crushed aggregates storehouse 3.
Further, in the above scheme, one side of the infusion tube 36 is fixedly connected with a liquid outlet tube 39, one end of the liquid outlet tube 39 is connected with the high-pressure pump 33, the top end of the high-pressure pump 33 is fixedly connected with a data line, one end of the data line is connected with the controller 34, one side of the controller 34 is fixedly connected with a conducting wire, one end of the conducting wire extends into the enzyme reaction box 16 and is connected with the wide-angle camera 27, the controller 34 is electrically connected with the conducting wire and the data line, and the centrifugal motor 28 can be started and stopped through the arrangement of the controller 34.
When using, put into garrulous feed bin 3 with the placenta in, start the garrulous motor 8, the output of garrulous motor 8 passes through the shaft coupling and drives garrulous input shaft 9 and rotate, and garrulous input shaft 9 drives garrulous sword 11 and rotates, thereby garrulous sword 11 rotates and can cut up the placenta.
The fragmentation motor 8 is closed after the fragmentation, the high-pressure pump 33 is started, the biological enzyme in the biological enzyme storage tank 32 is discharged into the high-pressure pump 33 through the liquid inlet pipe 35, the biological enzyme is discharged into the liquid inlet pipe 39 through the pressurization effect of the high-pressure pump 33, then the biological enzyme is discharged into the liquid outlet pipe 39, the biological enzyme is discharged into the liquid conveying pipe 36 through the liquid outlet pipe 39, and finally the biological enzyme is sprayed onto the placenta through the porous spray nozzle 38, so that the biological enzyme reaction can be realized, meanwhile, the placenta particles attached to the inner wall can be cleaned, and the subsequent operation is facilitated.
Through outwards pulling semicircle handle 14 for ejection of compact baffle 13 outwards removes, thereby make the placenta after cutting up get into enzyme reaction case 16 in, start biax micromotor 22, biax micromotor 22 makes two centrifugal input shafts 23 rotate, two centrifugal input shafts 23 drive corresponding eccentric wheel 24 respectively and rotate, make reciprocating pump 19 drive enzyme reaction case 16 vibrations under the effect of high frequency spring 21, thereby can promote biological enzyme reaction through the vibrations of enzyme reaction case 16, accelerate dry cell separation rate.
The centrifugal motor 28 is started, the output end of the centrifugal motor 28 enables the input shaft to rotate through the coupler, the input shaft further enables the connecting disc 30 to rotate, the connecting disc 30 enables the enzyme reaction box 16 to rotate, liquid is thrown out through the separation hole 17 and the collection hole 31 into the collection bin 1 through the rotation of the enzyme reaction box 16, and therefore stem cells are separated more thoroughly in placenta tissues, integrated operation is achieved, and the phenomenon that different devices are replaced in a segmented mode is avoided, and the infection probability is increased.
Through the arrangement of the wide-angle camera 27, the separation hole 17 can correspond to the position of the fit lug 26 after the enzyme reaction box 16 stops rotating, and after the correspondence, a signal is transmitted into the controller 34 through a conducting wire, and the centrifugal motor 28 is turned off through the controller 34.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. The utility model provides a stem cell intelligence extraction equipment, includes collection storehouse (1), its characterized in that: collect top fixedly connected with separation reaction storehouse (2) in storehouse (1), the garrulous feed bin of top fixedly connected with (3) in separation reaction storehouse (2), the top of garrulous feed bin (3) is equipped with sealed lid (5), bottom fixedly connected with fragmentation motor (8) of sealed lid (5), the output fixedly connected with shaft coupling of fragmentation motor (8), the one end fixedly connected with fragmentation input shaft (9) of shaft coupling, one side fixedly connected with fragmentation sword (11) of fragmentation input shaft (9), blown down tank (12) have been seted up to one side inner wall of garrulous feed bin (3), sliding connection has ejection of compact baffle (13) in blown down tank (12).
2. The intelligent extraction equipment of stem cells as claimed in claim 1, wherein: the inner wall of the other side of the crushing bin (3) is provided with three rectangular limiting grooves (4) which are uniformly distributed; the top fixedly connected with arc handle (6) of sealed lid (5), the three rectangle stopper (7) of bottom fixedly connected with of sealed lid (5), it is three the position of rectangle stopper (7) respectively with three the position of rectangle spacing groove (4) is corresponding, and looks adaptation, and is three one side fixedly connected with that rectangle stopper (7) are close to each other agrees with dish (10), the commentaries on classics hole has been seted up at the top of agreeing with dish (10), the one end of fragmentation input shaft (9) is run through change the hole, and extend to outside the one side in change the hole.
3. The intelligent extraction equipment of stem cells as claimed in claim 2, wherein: the one end of ejection of compact baffle (13) extends to the outside of garrulous feed bin (3), the one end fixedly connected with semicircle handle (14) of ejection of compact baffle (13), the bottom fixedly connected with discharging pipe (15) of garrulous feed bin (3), the bottom of discharging pipe (15) extends to in the separation reaction storehouse (2), the bottom fixedly connected with enzyme reaction case (16) of discharging pipe (15), enzyme reaction case (16) are located in separation reaction storehouse (2).
4. The intelligent extraction equipment of stem cells as claimed in claim 3, wherein: a plurality of evenly distributed separation holes (17) are formed in one side of the enzyme reaction box (16), a guide column (18) is fixedly connected to the inner wall of the bottom of the enzyme reaction box (16), a vibration pump (19) is arranged on the top end of the guide column (18), an installation block (20) is fixedly connected to one side of the vibration pump (19), the position of the installation block (20) corresponds to the position of the guide column (18), and a high-frequency spring (21) is fixedly sleeved on one side of the guide column (18).
5. The intelligent extraction equipment of stem cells as claimed in claim 4, wherein: the both ends of high frequency spring (21) respectively with enzyme reaction case (16) with installation piece (20) are connected, the little motor (22) of fixedly connected with biax in vibrations pump (19), the equal fixedly connected with centrifugation input shaft (23) in both ends of the little motor (22) of biax, two the equal fixedly connected with eccentric wheel (24) in both ends of the mutual keeping away from of centrifugation input shaft (23), the outside of enzyme reaction case (16) is equipped with liquid collection frame (25).
6. The intelligent extraction equipment of stem cells as claimed in claim 5, wherein: liquid collection frame (25) are close to a plurality of even agreeing with lug (26) of distribution of one side fixedly connected with of enzyme reaction case (16), a plurality of even distributions agree with the position of lug (26) respectively with a plurality of even distributions the position of separation hole (17) corresponds each other, one side fixedly connected with wide-angle camera (27) of liquid collection frame (25), collect storehouse (1) internal fixedly connected with centrifugal motor (28).
7. The intelligent extraction equipment of stem cells of claim 6, characterized in that: the output fixedly connected with shaft coupling of centrifugal motor (28), the one end fixedly connected with input shaft of shaft coupling, it has seted up the rotation hole to collect storehouse (1) top, the position in rotation hole with the position of input shaft is corresponding, the one end of input shaft runs through rotate the hole, and extend to outside one side in rotation hole, the top fixedly connected with connection pad (30) of input shaft, the outside fixedly connected with annular division board (29) of centrifugal motor (28).
8. The intelligent extraction equipment of stem cells as claimed in claim 7, wherein: the top of connection pad (30) with enzyme reaction case (16) are connected, enzyme reaction case (16) with the collection hole (31) that same a plurality of distributions are even are seted up at the top of collecting storehouse (1), collect top fixedly connected with biological enzyme bin (32), high-pressure pump (33) and controller (34) of storehouse (1), the top fixedly connected with feed liquor pipe (35) of biological enzyme bin (32), the other end of feed liquor pipe (35) with high-pressure pump (33) are connected.
9. The intelligent extraction equipment of stem cells as claimed in claim 8, wherein: one side fixedly connected with transfer line (36) of high-pressure pump (33), annular spacing groove (40) and the even perforation of a plurality of distributions have been seted up in the outside of garrulous feed bin (3), fixedly connected with shunt tubes (37) in annular spacing groove (40), a plurality of even porous shower nozzles (38) of distribution of one side fixedly connected with of shunt tubes (37), the position of the even porous shower nozzle (38) of a plurality of distributions respectively with a plurality of the fenestrate position corresponds each other, and is a plurality of the one end of porous shower nozzle (38) runs through corresponding respectively the perforation, and extends to in one side of garrulous feed bin (3).
10. The intelligent extraction equipment of stem cells of claim 9, characterized in that: one side fixedly connected with drain pipe (39) of transfer line (36), the one end of drain pipe (39) with high-pressure pump (33) are connected, the top fixedly connected with data line of high-pressure pump (33), the one end of data line with controller (34) are connected, one side fixedly connected with conducting wire of controller (34), the one end of conducting wire extends to in enzyme reaction case (16), with wide angle camera (27) are connected, controller (34) with the conducting wire the data line is electric connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110512944.7A CN113308370A (en) | 2021-05-11 | 2021-05-11 | Intelligent extraction equipment and extraction method for stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110512944.7A CN113308370A (en) | 2021-05-11 | 2021-05-11 | Intelligent extraction equipment and extraction method for stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113308370A true CN113308370A (en) | 2021-08-27 |
Family
ID=77372829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110512944.7A Pending CN113308370A (en) | 2021-05-11 | 2021-05-11 | Intelligent extraction equipment and extraction method for stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113308370A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113943651A (en) * | 2021-11-17 | 2022-01-18 | 郭兴华 | Stem cell extraction centrifugal extraction process |
| CN117535121A (en) * | 2024-01-09 | 2024-02-09 | 四川博浩达生物科技有限公司 | Industrial enzyme sample extraction equipment |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4028190A (en) * | 1974-11-07 | 1977-06-07 | Merck & Co., Inc. | Apparatus for preparing isolated cells from tissue |
| CN209113875U (en) * | 2018-10-17 | 2019-07-16 | 上海杏家医疗科技有限公司 | A kind of placenta stem-cell extraction perifusion apparatus |
| CN209989380U (en) * | 2019-01-16 | 2020-01-24 | 卡替（上海）生物技术股份有限公司 | Stem cell extraction equipment based on dental pulp |
| CN213060835U (en) * | 2020-07-10 | 2021-04-27 | 姜帅 | Biological methane anaerobic fermentation equipment |
-
2021
- 2021-05-11 CN CN202110512944.7A patent/CN113308370A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4028190A (en) * | 1974-11-07 | 1977-06-07 | Merck & Co., Inc. | Apparatus for preparing isolated cells from tissue |
| CN209113875U (en) * | 2018-10-17 | 2019-07-16 | 上海杏家医疗科技有限公司 | A kind of placenta stem-cell extraction perifusion apparatus |
| CN209989380U (en) * | 2019-01-16 | 2020-01-24 | 卡替（上海）生物技术股份有限公司 | Stem cell extraction equipment based on dental pulp |
| CN213060835U (en) * | 2020-07-10 | 2021-04-27 | 姜帅 | Biological methane anaerobic fermentation equipment |
Non-Patent Citations (1)
| Title |
|---|
| 马立峰: "《普通高等教育十三五规划教材 矿山机械 下》", 31 March 2021, 冶金工业出版社, pages: 253 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113943651A (en) * | 2021-11-17 | 2022-01-18 | 郭兴华 | Stem cell extraction centrifugal extraction process |
| CN113943651B (en) * | 2021-11-17 | 2023-08-18 | 北京集美生物科技有限公司 | Stem cell extraction centrifugal extraction process |
| CN117535121A (en) * | 2024-01-09 | 2024-02-09 | 四川博浩达生物科技有限公司 | Industrial enzyme sample extraction equipment |
| CN117535121B (en) * | 2024-01-09 | 2024-03-19 | 四川博浩达生物科技有限公司 | Industrial enzyme sample extraction equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113308370A (en) | Intelligent extraction equipment and extraction method for stem cells | |
| JP3139358U (en) | Used paper diaper crushing and separating recovery device | |
| CN111849762B (en) | Stem cell extraction element | |
| CN210752928U (en) | Separator is smashed to solid for chemistry experiments | |
| CN220110561U (en) | Collecting assembly for sludge treatment | |
| CN210857072U (en) | Leaf cleans processor | |
| CN219292371U (en) | Kitchen garbage innocent treatment equipment | |
| CN208658645U (en) | A kind of fresh-keeping pressing device of fruit juice | |
| CN216678392U (en) | Medical waste spray type treatment and disinfection device | |
| CN208786346U (en) | A kind of probiotics prilling granulator | |
| CN108855505A (en) | Sludge decontamination processing system | |
| CN212975021U (en) | Liquid preparation tank with filtering device | |
| CN213726918U (en) | Novel bio-pharmaceuticals dispersion devices convenient to ejection of compact | |
| CN109894236B (en) | Waste automobile crushing equipment convenient for dust removal | |
| CN208839722U (en) | A kind of medicinal herb grinder | |
| CN207468219U (en) | A kind of slit filter sewage grid maker | |
| CN101422667A (en) | Filter device in glucose production process | |
| CN108821523A (en) | Sludge treating system | |
| CN219072255U (en) | Nanometer inorganic catalyst production facility for plastic degradation | |
| CN201313010Y (en) | Filtering device after secondary decoloring in the anhydrous dextrose production process | |
| CN108821524A (en) | Sludge removes heavy metal treatment systems | |
| CN219091261U (en) | Natural plant advanced high-speed centrifugal auxiliary separator | |
| CN108310012A (en) | A kind of good airproof performance, cleaning easy to disassemble stem cell medicine preparing device | |
| CN109821853A (en) | A kind of technique of hospital's fixed-end forces | |
| CN217696230U (en) | ICU nursing patient food treatment facility |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |