CN113943651A - Stem cell extraction centrifugal extraction process - Google Patents
Stem cell extraction centrifugal extraction process Download PDFInfo
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- CN113943651A CN113943651A CN202111359789.6A CN202111359789A CN113943651A CN 113943651 A CN113943651 A CN 113943651A CN 202111359789 A CN202111359789 A CN 202111359789A CN 113943651 A CN113943651 A CN 113943651A
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Abstract
The invention discloses a stem cell extraction centrifugal extraction process, which is characterized by comprising the following steps: s1, designing a centrifugal extraction device; s2, when in extraction, an operator starts a motor of the centrifugal extraction device, the motor drives a first rotating shaft to rotate through a first gear and a second gear, and the first rotating shaft drives the extrusion wheel to eccentrically rotate by taking the first rotating shaft as an axis; the sliding column is always attached to the outer wall of the extrusion wheel under the action of the first spring, and when the extrusion wheel rotates, the sliding column is extruded rightwards, so that the sliding column is driven to slide rightwards on the inner wall of the liquid extraction shell and the first spring is extruded at the same time; s4, the gear drives the gear ring to rotate, so that the centrifugal cylinder is driven to rotate, the lantern ring is driven to rotate in the arc-shaped chute on the inner wall of the centrifugal shell, and digestive juice flows into the lower part of the intermittent blanking shell from the blanking hole and then falls into the lower part of the centrifugal cylinder; s5, when the residue in the centrifugal cylinder needs to be cleaned, the sealing plate is pushed downwards to move downwards and extrude the spring IV, and the sealing plate is separated from the bottom of the inner wall of the centrifugal cylinder.
Description
Technical Field
The invention relates to the technical field of stem cell extraction, in particular to a stem cell extraction centrifugal extraction process.
Background
The cells are multipotential cells with self-replication ability, can be differentiated into various functional cells under certain conditions, are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and are divided into three types according to the development potentiality of the stem cells: the stem cell is an insufficiently differentiated and immature cell, has the potential function of regenerating various tissues, organs and human bodies, and is called as a universal cell in the medical field.
In the prior art, when placenta stem cells are extracted, most of minced placenta fragments are placed in a container to naturally react with corresponding biological enzymes, then the cells are centrifuged, and digestive juice and placenta residues are subjected to solid-liquid separation. The placenta piece of current device mixes inhomogeneously with dissolving enzyme, leads to the digestion effect poor, influences the cell and draws the effect, and digestion reaction and cytocentrifuge are mostly separated, lead to cell extraction efficiency slower.
Therefore, we disclose a stem cell extraction centrifugation extraction process to solve the above problems.
Disclosure of Invention
The invention aims to provide a centrifugal extraction process for extracting stem cells, which has the advantages of automatic addition of collagenase, uniform mixing, good digestion effect, synchronization of digestion reaction and cell centrifugation and high cell extraction efficiency, and solves the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a stem cell extraction centrifugation extraction process is characterized by comprising the following steps:
s1, designing a centrifugal extraction device;
s2, when in extraction, an operator starts a motor (5) of the centrifugal extraction device, the motor drives a first rotating shaft (7) to rotate through a first gear (6) and a second gear (8), and the first rotating shaft drives an extrusion wheel (9) to eccentrically rotate by taking the first rotating shaft (7) as an axis;
the sliding column (13) is always attached to the outer wall of the extrusion wheel (9) under the action of the first spring (20), when the extrusion wheel rotates, the sliding column (13) is extruded rightwards, the sliding column is driven to slide rightwards on the inner wall of the liquid pumping shell (12) and meanwhile the first spring (20) is extruded, the pressure of the inner cavity of the liquid storage shell (14) is increased, the second sealing ball (26) and the first sealing ball (23) are simultaneously under the pressure action of a collagenase solution in the liquid storage shell (14), the second sealing ball (26) starts to move towards the liquid guide pipe (16) under the hydraulic action and simultaneously compresses the third spring (25), the outer wall of the second sealing ball is not attached to the inner wall of the liquid storage shell (14), and the collagenase solution in the inner cavity of the liquid storage shell (14) is extruded into the inner cavity of the liquid guide pipe (16) and is guided into the centrifugal shell (3); when the sliding column (13) moves leftwards, the interior of the liquid storage shell (14) is in a negative pressure state, the collagenase solution in the liquid storage tank (17) is pumped into the liquid storage shell (14) through the liquid pumping pipe (15), and automatic liquid supplementation is realized, so that the collagenase solution is automatically pumped;
s3, throwing the placenta fragments into the centrifugal shell (3), and leading the placenta fragments and the collagenase solution into the feeding cover (4) and then into the intermittent feeding shell (37) for digestion reaction;
s4, a gear IV (28) drives a gear ring (29) to rotate, so that a centrifugal cylinder (30) is driven to rotate, a lantern ring (32) is driven to rotate in an arc-shaped chute in the inner wall of a centrifugal shell (3), digestive juice flows into the lower part of an intermittent blanking shell (37) from a blanking hole (39) and then falls into the lower part of the centrifugal cylinder (30), centrifugal force is generated when the centrifugal cylinder rotates, the digestive juice is thrown out of the centrifugal cylinder (30) through a water outlet hole (31) under the centrifugal force action of the centrifugal cylinder (30), and placenta residues are left in the centrifugal cylinder (30), so that centrifugal separation of the digestive juice and placenta residues is realized; after being thrown out of the centrifugal cylinder (30), the digestive juice flows out of a liquid outlet pipe (19) on the outer wall of the centrifugal shell (3) and is collected in a centralized way through a collection box (18), so that the subsequent purification operation is facilitated;
s5, when the residue in the centrifugal cylinder (30) needs to be cleaned, the sealing plate (33) is pressed downwards to move downwards and extrude the spring IV (35), and at the moment, the sealing plate (33) is separated from the bottom of the inner wall of the centrifugal cylinder (30).
In the scheme, the centrifugal extraction device comprises a mounting bottom plate (1), wherein a support column (2) is fixedly connected to the upper surface of the mounting bottom plate (1), and a centrifugal shell (3) is fixedly connected to the top of the support column (2); the top of the mounting bottom plate (1) is fixedly connected with a collecting box (18) relative to the right side position of the centrifugal shell (3), and the right side wall of the centrifugal shell (3) is fixedly connected with a liquid outlet pipe (19); one end of the liquid outlet pipe (19) far away from the centrifugal shell (3) extends into the collection box (18), a feeding cover (4) used for throwing placenta fragments and adding collagenase solution is arranged at the top of the centrifugal shell (3), and a motor (5) is fixedly arranged at the top of the centrifugal shell (3); the output shaft of the motor (5) is fixedly connected with a first gear (6), the upper surface of the centrifugal shell (3) is provided with a through hole, and the inner wall of the through hole is fixedly connected with a first rotating shaft (7) in a rotating mode.
In the scheme, the bottom end of the first rotating shaft (7) extends into the centrifugal shell (3) and is fixedly connected with a third gear (10), the top end of the first rotating shaft (7) is fixedly connected with an extrusion wheel (9), the top end of the first rotating shaft (7) is positioned at an eccentric position at the bottom of the extrusion wheel (9), and the outer wall of the first rotating shaft (7) is provided with a second gear (8) which is meshed with the first gear (6); a positioning plate (11) is fixedly mounted at the top of the mounting base plate (1), a through hole is formed in the inner wall of the positioning plate (11), a liquid pumping shell (12) is fixedly mounted on the inner wall of the through hole, a sliding column (13) is connected to the inner wall of the liquid pumping shell (12) in a sliding mode, the left side wall of the sliding column (13) is of an arc-shaped structure and is attached to the outer wall of the extrusion wheel (9), and a first spring (20) is arranged between the right side wall of the sliding column (13) and the inner wall of the liquid pumping shell (12);
the right side wall of the liquid pumping shell (12) is fixedly connected with a liquid storage shell (14), the rear side of the inner wall of the liquid storage shell (14) is fixedly connected with a first supporting rod (21), the back of the first supporting rod (21) is fixedly connected with a second spring (22), and the back of the second spring (22) is fixedly connected with a first sealing ball (23); a liquid pumping pipe (15) is fixedly connected to the back of the liquid storage shell (14), one end, far away from the liquid storage shell (14), of the liquid pumping pipe (15) is fixedly connected with a liquid storage box (17), the bottom of the liquid storage box (17) is fixedly connected with the upper surface of the mounting bottom plate (1), and a second supporting rod (24) is fixedly connected to the front side of the inner wall of the liquid storage shell (14); the back of the second supporting rod (24) is fixedly connected with a third spring (25), and the back of the third spring (25) is fixedly connected with a second sealing ball (26); the liquid feeding device is characterized in that a liquid guide pipe (16) is fixedly connected to the front face of the liquid storage shell (14), one end, far away from the liquid storage shell (14), of the liquid guide pipe (16) extends to the upper portion of the feeding cover (4), a centrifugal cylinder (30) is arranged in the centrifugal shell (3), a lantern ring (32) is fixedly connected to the bottom of the outer wall of the centrifugal cylinder (30), and an arc-shaped sliding groove for the lantern ring (32) to be in sliding connection is formed in the inner wall of the centrifugal shell (3).
In the scheme, a sealing plate (33) is connected to the bottom of the inner wall of the centrifugal cylinder (30) in a sliding mode, a spring IV (35) is arranged between the middle position of the bottom of the sealing plate (33) and the top of the mounting bottom plate (1), and telescopic rods (34) with limiting functions are arranged on the left side and the right side of the bottom of the sealing plate (33) relative to the spring IV (35); the top fixedly connected with connecting rod (38) of closing plate (33), the top of connecting rod (38) extends to the top and the fixedly connected with intermittent type unloading casing (37) of apopore (31), the top bore of intermittent type unloading casing (37) is greater than the bottom bore of intermittent type unloading casing (37), and the outer wall top of intermittent type unloading casing (37) and the inner wall laminating of centrifuge tube (30), two sets of blanking holes (39) have been seted up to the inner wall bottom of intermittent type unloading casing (37).
In the scheme, the bottom of the gear III (10) is fixedly connected with a rotating shaft III (40), and the bottom end of the rotating shaft III (40) is fixedly connected with two groups of baffle plates (41) for closing the two groups of blanking holes (39); baffle (41) are fan-shaped structure, the lower surface of baffle (41) and the laminating of the inner wall bottom of intermittent type unloading casing (37).
In the scheme, the left side and the right side of the bottom end of the rotating shaft III (40) are fixedly connected with scraping pieces (42), the scraping pieces (42) are of bent structures, and the bottoms of the scraping pieces (42) are attached to the inner wall of the intermittent blanking shell (37).
In step S3, the first rotating shaft (7) drives the fourth gear (28) to rotate through the third gear (10), and simultaneously drives the second rotating shaft (27) and the stirring blades (36) to rotate, and the stirring blades (36) stir the placenta fragments and the collagenase solution thrown into the intermittent blanking shell (37), so as to accelerate the digestion reaction speed of the placenta fragments and the collagenase solution.
In step S3, the third gear (10) drives the third baffle (41) to rotate through the third rotating shaft (40), and when the third baffle is displaced above the blanking hole (39), the blanking hole (39) is closed, and at this time, the digestive juice formed by the placenta fragments and the collagenase solution cannot flow out of the blanking hole (39); the blanking holes (39) are gradually exposed along with the continuous rotation of the baffle (41), and at the moment, the digestive juice flows into the lower part of the intermittent blanking shell (37) from the blanking holes (39), and the circulation is carried out, so that the intermittent discharge is realized, and the placenta fragments and the collagenase solution can be fully digested and reacted in the intermittent blanking shell (37).
The beneficial effects are as follows:
1. when the extrusion wheel rotates, the sliding column can be extruded rightwards, the sliding column is further driven to slide rightwards on the inner wall of the liquid pumping shell, the spring I is extruded simultaneously, the pressure of the inner cavity of the liquid storage shell is increased, the sealing ball body II and the sealing ball body I are simultaneously under the pressure action of collagenase solution in the liquid storage shell, the sealing ball body II starts to move towards the liquid guide pipe due to the hydraulic action at the moment, the spring III is compressed simultaneously, the outer wall of the sealing ball body II is not attached to the inner wall of the liquid storage shell any more at the moment, the collagenase solution in the inner cavity of the liquid storage shell is extruded into the inner cavity of the liquid guide pipe and is guided into the centrifugal shell, and on the contrary, when the sliding column moves leftwards, the liquid storage shell is in a negative pressure state at the moment, and then collagenase solution in the liquid storage box is pumped into the liquid storage shell through the liquid suction pipe, automatic liquid replenishing is achieved, and automatic liquid extraction is achieved.
2. The three synchronous rotations of the gear are driven through the rotation of the rotating shaft, the gear four which is meshed with the gear three is driven to rotate, the rotating shaft two and the stirring fan blades are driven to rotate simultaneously, and the placenta fragments and the collagenase solution which are thrown into the intermittent blanking shell are stirred through the three groups of stirring fan blades, so that the digestion reaction speed of the placenta fragments and the collagenase solution is accelerated.
3. The three gears can drive the rotating shaft to rotate synchronously when rotating, the baffle is driven to rotate, the bottom of the baffle is attached to the bottom of the inner wall of the intermittent blanking shell, when the baffle is displaced to the upper side of the blanking hole, the blanking hole is sealed, digestion liquid formed by the placenta fragments and the collagenase solution cannot flow out of the blanking hole, the blanking hole is exposed gradually along with the continuous rotation of the baffle, the digestion liquid flows into the lower portion of the intermittent blanking shell from the blanking hole, the circulation is carried out, the intermittent discharging function is realized, and the placenta fragments and the collagenase solution can be subjected to full digestion reaction in the intermittent blanking shell.
4. The three groups of gears rotate four times to drive a gear ring meshed with the gears to rotate synchronously, so that the centrifugal cylinder is driven to rotate, the lantern ring is driven to rotate in an arc-shaped chute formed in the inner wall of the centrifugal shell, digestive juice flows into the lower portion of the intermittent blanking shell from the blanking hole and then falls into the lower portion of the centrifugal cylinder, a certain centrifugal force is generated when the centrifugal cylinder rotates, the digestive juice penetrates through the water outlet hole under the centrifugal force action of the centrifugal cylinder and is thrown out of the centrifugal cylinder, placenta residues are remained in the centrifugal cylinder, and centrifugal separation of the digestive juice and the placenta residues is achieved.
Drawings
FIG. 1 is a schematic diagram of a centrifugal pumping device according to the present invention;
FIG. 2 is a schematic view of an extrusion wheel of the centrifugal pumping device of the present invention;
FIG. 3 is a schematic view of a configuration of a slide column of the centrifugal pumping device used in the present invention;
FIG. 4 is a schematic diagram of a centrifuge bowl of the centrifugal pumping device of the present invention;
FIG. 5 is a schematic view of the intermittent blanking shell structure of the centrifugal pumping device used in the present invention;
FIG. 6 is a schematic view of a baffle structure of a centrifugal pumping device used in the present invention;
FIG. 7 is a schematic view of the structure of the blanking hole of the centrifugal extracting device used in the present invention.
In the figure: 1. mounting a bottom plate; 2. a support pillar; 3. a centrifugal housing; 4. a feed hood; 5. a motor; 6. a first gear; 7. a first rotating shaft; 8. a second gear; 9. an extrusion wheel; 10. a third gear; 11. positioning a plate; 12. a liquid pumping shell; 13. a traveler; 14. a liquid storage housing; 15. a liquid pumping pipe; 16. a catheter; 17. a liquid storage tank; 18. a collection box; 19. a liquid outlet pipe; 20. a first spring; 21. a first supporting rod; 22. a second spring; 23. sealing the first sphere; 24. a second supporting rod; 25. a third spring; 26. a second sealing ball body; 27. a second rotating shaft; 28. a fourth gear; 29. a toothed ring; 30. a centrifugal cylinder; 31. a water outlet hole; 32. a collar; 33. a sealing plate; 34. a telescopic rod; 35. a fourth spring; 36. a stirring fan blade; 37. intermittent blanking of the shell; 38. a connecting rod; 39. a blanking hole; 40. a rotating shaft III; 41. a baffle plate; 42. and (4) scraping the blade.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
A stem cell extraction centrifugation extraction process is characterized by comprising the following steps:
s1, designing a centrifugal extraction device;
the first embodiment is as follows:
as shown in fig. 1-2, a centrifugal extracting device comprises a mounting base plate 1, a supporting column 2 is fixedly connected to the upper surface of the mounting base plate 1, a centrifugal shell 3 is fixedly connected to the top of the supporting column 2, a collecting box 18 is fixedly connected to the top of the mounting base plate 1 at a position corresponding to the right side of the centrifugal shell 3, a liquid outlet pipe 19 is fixedly connected to the right side wall of the centrifugal shell 3, one end of the liquid outlet pipe 19 away from the centrifugal shell 3 extends into the collecting box 18, a feeding cover 4 for throwing placenta fragments and adding collagenase solution is arranged at the top of the centrifugal shell 3, a motor 5 is fixedly mounted at the top of the centrifugal shell 3, a first gear 6 is fixedly connected to an output shaft of the motor 5, a through hole is formed in the upper surface of the centrifugal shell 3, a first rotating shaft 7 is rotatably connected to the inner wall of the through hole in a fixed shaft manner, a bottom end of the first rotating shaft 7 extends into the centrifugal shell 3 and is fixedly connected with a third gear 10, the top end of the first rotating shaft 7 is fixedly connected with an extrusion wheel 9, the top end of the first rotating shaft 7 is located at an eccentric position of the bottom of the extrusion wheel 9, and a second gear 8 meshed with the first gear 6 is arranged on the outer wall of the first rotating shaft 7.
The motor 5 is started, the first gear 6 is driven to rotate through the motor 5, the second gear 8 meshed with the first gear 6 is driven to rotate, the first rotating shaft 7 is driven to rotate, the extrusion wheel 9 is driven to rotate through the rotation of the first rotating shaft 7, and due to the fact that the top end of the first rotating shaft 7 is located at the eccentric position of the bottom of the extrusion wheel 9, when the first rotating shaft 7 rotates, the extrusion wheel 9 can rotate eccentrically by taking the first rotating shaft 7 as an axis.
As shown in fig. 3, preferably, a positioning plate 11 is fixedly mounted at the top of the mounting base plate 1, a through hole is formed in the inner wall of the positioning plate 11, a liquid pumping housing 12 is fixedly mounted on the inner wall of the through hole, a sliding column 13 is slidably connected to the inner wall of the liquid pumping housing 12, the left side wall of the sliding column 13 is of an arc-shaped structure and is attached to the outer wall of the squeezing wheel 9, and a first spring 20 is arranged between the right side wall of the sliding column 13 and the inner wall of the liquid pumping housing 12.
The sliding column 13 is always attached to the outer wall of the extrusion wheel 9 under the action of elastic potential energy of the first spring 20, and when the extrusion wheel 9 rotates, the sliding column 13 is extruded rightwards, so that the sliding column 13 is driven to slide rightwards on the inner wall of the liquid pumping shell 12, and the first spring 20 is extruded at the same time.
As shown in fig. 3, the right side wall of the liquid pumping housing 12 is fixedly connected with a liquid storage housing 14, the rear side of the inner wall of the liquid storage housing 14 is fixedly connected with a first support rod 21, the back of the first support rod 21 is fixedly connected with a second spring 22, the back of the second spring 22 is fixedly connected with a first sealing ball 23, the aperture of the contact position of the liquid storage housing 14 and the first sealing ball 23 is the same as the diameter of the first sealing ball 23, the back of the liquid storage housing 14 is fixedly connected with a liquid pumping pipe 15, one end of the liquid pumping pipe 15 far away from the liquid storage housing 14 is fixedly connected with a liquid storage tank 17, and the bottom of the liquid storage tank 17 is fixedly connected with the upper surface of the mounting base plate 1.
When the sliding column 13 moves leftwards, the interior of the liquid storage shell 14 is in a negative pressure state, and then the collagenase solution in the liquid storage tank 17 is pumped into the liquid storage shell 14 through the liquid pumping pipe 15, so that the collagenase solution is automatically replenished, and the collagenase solution is automatically pumped.
As shown in fig. 3, a second support rod 24 is fixedly connected to the front side of the inner wall of the liquid storage housing 14, a third spring 25 is fixedly connected to the back of the second support rod 24, a second sealing ball 26 is fixedly connected to the back of the third spring 25, the aperture of the contact position of the liquid storage housing 14 and the second sealing ball 26 is the same as the diameter of the second sealing ball 26, a liquid guide tube 16 is fixedly connected to the front of the liquid storage housing 14, and one end of the liquid guide tube 16, which is far away from the liquid storage housing 14, extends to the upper side of the feeding cover 4.
At the moment, the pressure intensity of the inner cavity of the liquid storage shell 14 is increased, the second sealing ball 26 and the first sealing ball 23 are simultaneously under the pressure action of the collagenase solution in the liquid storage shell 14, at the moment, the second sealing ball 26 starts to move towards the liquid guide pipe 16 under the hydraulic action, meanwhile, the third spring 25 is compressed, at the moment, the outer wall of the second sealing ball 26 is not attached to the inner wall of the liquid storage shell 14 any more, and the collagenase solution in the inner cavity of the liquid storage shell 14 is extruded into the inner cavity of the liquid guide pipe 16 and is guided into the centrifugal shell 3.
As shown in fig. 5, a centrifuge tube 30 is disposed in the centrifuge housing 3, a lantern ring 32 is fixedly connected to the bottom of the outer wall of the centrifuge tube 30, an arc-shaped chute for slidably connecting the lantern ring 32 is formed in the inner wall of the centrifuge housing 3, a water outlet hole 31 is formed in the outer wall of the centrifuge tube 30 above the lantern ring 32, a toothed ring 29 is fixedly connected to the top of the centrifuge tube 30, three sets of second rotating shafts 27 arranged in an annular shape are rotatably connected to the top of the inner wall of the centrifuge housing 3 in a fixed-axis manner, a fourth gear 28 meshed with the third gear 10 and the toothed ring 29 is fixedly connected to the outer wall of the second rotating shaft 27, and stirring blades 36 for stirring placenta fragments and collagenase solution are fixedly connected to the bottom of the second rotating shafts 27.
The rotation of the three groups of gears four 28 can drive the gear ring 29 meshed with the gears four 28 to synchronously rotate, so as to drive the centrifugal cylinder 30 to rotate, meanwhile, the lantern ring 32 is driven to rotate in an arc-shaped chute formed in the inner wall of the centrifugal shell 3, digestive juice flows into the lower part of the intermittent blanking shell 37 from the blanking hole 39 and then falls into the lower part of the centrifugal cylinder 30, a certain centrifugal force is generated when the centrifugal cylinder 30 rotates, the digestive juice is thrown out of the centrifugal cylinder 30 through the water outlet hole 31 under the centrifugal force of the centrifugal cylinder 30, placenta residues are remained in the centrifugal cylinder 30, and the centrifugal separation of the digestive juice and the placenta residues is realized.
As shown in fig. 5, a sealing plate 33 is slidably connected to the bottom of the inner wall of the centrifuge tube 30, a spring four 35 is disposed between the bottom middle position of the sealing plate 33 and the top of the mounting base plate 1, telescopic rods 34 with limiting function are disposed on the left and right sides of the bottom of the sealing plate 33 relative to the spring four 35, a connecting rod 38 is fixedly connected to the top of the sealing plate 33, the top end of the connecting rod 38 extends to the upper side of the water outlet hole 31 and is fixedly connected with an intermittent blanking shell 37, the top end caliber of the intermittent blanking shell 37 is greater than the bottom end caliber of the intermittent blanking shell 37, the top of the outer wall of the intermittent blanking shell 37 is attached to the inner wall of the centrifuge tube 30, and two sets of blanking holes 39 are disposed at the bottom of the inner wall of the intermittent blanking shell 37.
When the residue in the centrifuge tube 30 needs to be cleaned, the sealing plate 33 is pushed downwards only, so that the sealing plate 33 moves downwards and extrudes the spring IV 35, and at the moment, the sealing plate 33 is separated from the bottom of the inner wall of the centrifuge tube 30, so that the placenta residue above the sealing plate 33 can be taken out conveniently.
As shown in fig. 6, a third rotating shaft 40 is fixedly connected to the bottom of the third gear 10, two sets of baffles 41 for closing the two sets of blanking holes 39 are fixedly connected to the bottom end of the third rotating shaft 40, the baffles 41 are fan-shaped, and the lower surfaces of the baffles 41 are attached to the bottom of the inner wall of the intermittent blanking shell 37.
When the third gear 10 rotates, the third rotating shaft 40 is driven to synchronously rotate, the baffle 41 is driven to rotate, the bottom of the baffle 41 is attached to the bottom of the inner wall of the intermittent blanking shell 37, when the baffle 41 is displaced to the position above the blanking hole 39, the blanking hole 39 starts to be sealed, at the moment, digestive juice formed by placenta fragments and collagenase solution cannot flow out of the blanking hole 39, the blanking hole 39 is gradually exposed along with the continuous rotation of the baffle 41, and at the moment, the digestive juice flows into the position below the intermittent blanking shell 37 from the blanking hole 39, the circulation is carried out, the intermittent discharging function is realized, and the placenta fragments and the collagenase solution can be sufficiently digested and reacted in the intermittent blanking shell 37.
Example two:
as shown in fig. 6, on the basis of the first embodiment, further expansion is performed: the left side and the right side of the bottom end of the third rotating shaft 40 are fixedly connected with scraping pieces 42, the scraping pieces 42 are of bending structures, and the bottoms of the scraping pieces 42 are attached to the inner wall of the intermittent blanking shell 37.
Rotate through three 40 of pivot and can drive baffle 41 and rotate, because the lower surface of baffle 41 and the inner wall laminating of intermittent type unloading casing 37, can strike off the adnexed placenta residue of intermittent type unloading casing 37 inner wall when baffle 41 rotates, can realize self-cleaning intermittent type unloading casing 37 inner wall and automatic propelling movement placenta residue with placenta residue propelling movement to the blanking hole 39 who exposes in the while.
S2, when in extraction, an operator starts a motor 5 of the centrifugal extraction device, the motor drives a first rotating shaft 7 to rotate through a first gear 6 and a second gear 8, and the first rotating shaft drives an extrusion wheel 9 to eccentrically rotate by taking the first rotating shaft 7 as an axis;
the sliding column 13 is always attached to the outer wall of the extrusion wheel 9 under the action of the first spring 20, when the extrusion wheel rotates, the sliding column 13 is extruded rightwards, the sliding column is driven to slide rightwards on the inner wall of the liquid pumping shell 12, the first spring 20 is extruded at the same time, the pressure of the inner cavity of the liquid storage shell 14 is increased at the moment, the second sealing ball 26 and the first sealing ball 23 are simultaneously subjected to the pressure action of the collagenase solution in the liquid storage shell 14, at the moment, the second sealing ball 26 starts to move towards the direction of the liquid guide pipe 16 under the hydraulic action, the third spring 25 is compressed at the same time, the outer wall of the second sealing ball is not attached to the inner wall of the liquid storage shell 14 at the moment, and the collagenase solution in the inner cavity of the liquid storage shell 14 is extruded into the inner cavity of the liquid guide pipe 16 and is guided into the centrifugal shell 3; when the sliding column 13 moves leftwards, the interior of the liquid storage shell 14 is in a negative pressure state, the collagenase solution in the liquid storage tank 17 is pumped into the liquid storage shell 14 through the liquid pumping pipe 15, liquid is automatically replenished, and automatic extraction of the collagenase solution is realized;
s3, throwing the placenta fragments into the centrifugal shell 3, and leading the placenta fragments and the collagenase solution into the intermittent blanking shell 37 from the position of the feeding cover 4 for digestion reaction;
in step S3, the first rotating shaft 7 drives the fourth gear 28 to rotate through the third gear 10, and simultaneously drives the second rotating shaft 27 and the stirring blades 36 to rotate, and the stirring blades 36 stir the placenta fragments and the collagenase solution put into the intermittent blanking housing 37, so as to accelerate the digestion reaction speed of the placenta fragments and the collagenase solution.
In step S3, the gear wheel three 10 drives the baffle 41 to rotate through the rotating shaft three 40, and when the baffle is displaced above the blanking hole 39, the blanking hole 39 starts to be sealed, and at this time, the digestive juice formed by the placenta fragments and the collagenase solution cannot flow out of the blanking hole 39; the blanking holes 39 are gradually exposed as the baffle 41 continues to rotate, and then the digestive juice flows into the lower part of the intermittent blanking shell 37 from the blanking holes 39, and the circulation is carried out, so that the intermittent discharge is realized, and the placenta fragments and the collagenase solution can be fully digested and reacted in the intermittent blanking shell 37.
S4, the gear IV 28 drives the gear ring 29 to rotate, further drives the centrifuge tube 30 to rotate, and simultaneously drives the lantern ring 32 to rotate in the arc-shaped chute on the inner wall of the centrifuge shell 3, digestive juice flows into the lower part of the intermittent blanking shell 37 from the blanking hole 39 and then falls into the lower part of the centrifuge tube 30, centrifugal force is generated when the centrifuge tube rotates, the digestive juice is thrown out of the centrifuge tube 30 through the water outlet hole 31 under the centrifugal force action of the centrifuge tube 30, and placenta residues are left in the centrifuge tube 30, so that centrifugal separation of the digestive juice and placenta residues is realized; after being thrown out of the centrifugal cylinder 30, the digestive juice flows out of the liquid outlet pipe 19 on the outer wall of the centrifugal shell 3 and is collected in a centralized way by the collecting box 18, so that the subsequent purification operation is convenient;
s5, when the residue in the centrifuge tube 30 needs to be cleaned, the sealing plate 33 is pushed downwards to move downwards and extrude the spring IV 35, at this time, the sealing plate 33 is separated from the bottom of the inner wall of the centrifuge tube 30, and the placenta residue above the sealing plate 33 is taken out.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations may be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A stem cell extraction centrifugation extraction process is characterized by comprising the following steps:
s1, designing a centrifugal extraction device;
s2, when in extraction, an operator starts a motor (5) of the centrifugal extraction device, the motor drives a first rotating shaft (7) to rotate through a first gear (6) and a second gear (8), and the first rotating shaft drives an extrusion wheel (9) to eccentrically rotate by taking the first rotating shaft (7) as an axis;
the sliding column (13) is always attached to the outer wall of the extrusion wheel (9) under the action of the first spring (20), when the extrusion wheel rotates, the sliding column (13) is extruded rightwards, the sliding column is driven to slide rightwards on the inner wall of the liquid pumping shell (12) and meanwhile the first spring (20) is extruded, the pressure of the inner cavity of the liquid storage shell (14) is increased, the second sealing ball (26) and the first sealing ball (23) are simultaneously under the pressure action of a collagenase solution in the liquid storage shell (14), the second sealing ball (26) starts to move towards the liquid guide pipe (16) under the hydraulic action and simultaneously compresses the third spring (25), the outer wall of the second sealing ball is not attached to the inner wall of the liquid storage shell (14), and the collagenase solution in the inner cavity of the liquid storage shell (14) is extruded into the inner cavity of the liquid guide pipe (16) and is guided into the centrifugal shell (3); when the sliding column (13) moves leftwards, the interior of the liquid storage shell (14) is in a negative pressure state, the collagenase solution in the liquid storage tank (17) is pumped into the liquid storage shell (14) through the liquid pumping pipe (15), and automatic liquid supplementation is realized, so that the collagenase solution is automatically pumped;
s3, throwing the placenta fragments into the centrifugal shell (3), and leading the placenta fragments and the collagenase solution into the feeding cover (4) and then into the intermittent feeding shell (37) for digestion reaction;
s4, a gear IV (28) drives a gear ring (29) to rotate, so that a centrifugal cylinder (30) is driven to rotate, a lantern ring (32) is driven to rotate in an arc-shaped chute in the inner wall of a centrifugal shell (3), digestive juice flows into the lower part of an intermittent blanking shell (37) from a blanking hole (39) and then falls into the lower part of the centrifugal cylinder (30), centrifugal force is generated when the centrifugal cylinder rotates, the digestive juice is thrown out of the centrifugal cylinder (30) through a water outlet hole (31) under the centrifugal force action of the centrifugal cylinder (30), and placenta residues are left in the centrifugal cylinder (30), so that centrifugal separation of the digestive juice and placenta residues is realized; after being thrown out of the centrifugal cylinder (30), the digestive juice flows out of a liquid outlet pipe (19) on the outer wall of the centrifugal shell (3) and is collected in a centralized way through a collection box (18), so that the subsequent purification operation is facilitated;
s5, when the residue in the centrifugal cylinder (30) needs to be cleaned, the sealing plate (33) is pressed downwards to move downwards and extrude the spring IV (35), at the moment, the sealing plate (33) is separated from the bottom of the inner wall of the centrifugal cylinder (30), and the placenta residue above the sealing plate (33) is taken out.
2. The stem cell extraction centrifugation extraction process as claimed in claim 1, wherein: the centrifugal extraction device comprises a mounting bottom plate (1), a support column (2) is fixedly connected to the upper surface of the mounting bottom plate (1), and a centrifugal shell (3) is fixedly connected to the top of the support column (2); the top of the mounting bottom plate (1) is fixedly connected with a collecting box (18) relative to the right side position of the centrifugal shell (3), and the right side wall of the centrifugal shell (3) is fixedly connected with a liquid outlet pipe (19); one end of the liquid outlet pipe (19) far away from the centrifugal shell (3) extends into the collection box (18), a feeding cover (4) used for throwing placenta fragments and adding collagenase solution is arranged at the top of the centrifugal shell (3), and a motor (5) is fixedly arranged at the top of the centrifugal shell (3); the output shaft of the motor (5) is fixedly connected with a first gear (6), the upper surface of the centrifugal shell (3) is provided with a through hole, and the inner wall of the through hole is fixedly connected with a first rotating shaft (7) in a rotating mode.
3. The stem cell extraction centrifugation extraction process as claimed in claim 2, wherein: the bottom end of the first rotating shaft (7) extends into the centrifugal shell (3) and is fixedly connected with a third gear (10), the top end of the first rotating shaft (7) is fixedly connected with an extrusion wheel (9), the top end of the first rotating shaft (7) is located at an eccentric position of the bottom of the extrusion wheel (9), and a second gear (8) meshed with the first gear (6) is arranged on the outer wall of the first rotating shaft (7); a positioning plate (11) is fixedly mounted at the top of the mounting base plate (1), a through hole is formed in the inner wall of the positioning plate (11), a liquid pumping shell (12) is fixedly mounted on the inner wall of the through hole, a sliding column (13) is connected to the inner wall of the liquid pumping shell (12) in a sliding mode, the left side wall of the sliding column (13) is of an arc-shaped structure and is attached to the outer wall of the extrusion wheel (9), and a first spring (20) is arranged between the right side wall of the sliding column (13) and the inner wall of the liquid pumping shell (12);
the right side wall of the liquid pumping shell (12) is fixedly connected with a liquid storage shell (14), the rear side of the inner wall of the liquid storage shell (14) is fixedly connected with a first supporting rod (21), the back of the first supporting rod (21) is fixedly connected with a second spring (22), and the back of the second spring (22) is fixedly connected with a first sealing ball (23); a liquid pumping pipe (15) is fixedly connected to the back of the liquid storage shell (14), one end, far away from the liquid storage shell (14), of the liquid pumping pipe (15) is fixedly connected with a liquid storage box (17), the bottom of the liquid storage box (17) is fixedly connected with the upper surface of the mounting bottom plate (1), and a second supporting rod (24) is fixedly connected to the front side of the inner wall of the liquid storage shell (14); the back of the second supporting rod (24) is fixedly connected with a third spring (25), and the back of the third spring (25) is fixedly connected with a second sealing ball (26); the liquid feeding device is characterized in that a liquid guide pipe (16) is fixedly connected to the front face of the liquid storage shell (14), one end, far away from the liquid storage shell (14), of the liquid guide pipe (16) extends to the upper portion of the feeding cover (4), a centrifugal cylinder (30) is arranged in the centrifugal shell (3), a lantern ring (32) is fixedly connected to the bottom of the outer wall of the centrifugal cylinder (30), and an arc-shaped sliding groove for the lantern ring (32) to be in sliding connection is formed in the inner wall of the centrifugal shell (3).
4. The stem cell extraction centrifugation extraction process as claimed in claim 3, wherein: a sealing plate (33) is connected to the bottom of the inner wall of the centrifugal cylinder (30) in a sliding mode, a spring IV (35) is arranged between the middle position of the bottom of the sealing plate (33) and the top of the mounting bottom plate (1), and telescopic rods (34) with limiting functions are arranged on the left side and the right side of the bottom of the sealing plate (33) opposite to the spring IV (35); the top fixedly connected with connecting rod (38) of closing plate (33), the top of connecting rod (38) extends to the top and the fixedly connected with intermittent type unloading casing (37) of apopore (31), the top bore of intermittent type unloading casing (37) is greater than the bottom bore of intermittent type unloading casing (37), and the outer wall top of intermittent type unloading casing (37) and the inner wall laminating of centrifuge tube (30), two sets of blanking holes (39) have been seted up to the inner wall bottom of intermittent type unloading casing (37).
5. The stem cell extraction centrifugation extraction process as claimed in claim 3, wherein: the bottom of the third gear (10) is fixedly connected with a third rotating shaft (40), and the bottom end of the third rotating shaft (40) is fixedly connected with two groups of baffles (41) for closing the two groups of blanking holes (39); baffle (41) are fan-shaped structure, the lower surface of baffle (41) and the laminating of the inner wall bottom of intermittent type unloading casing (37).
6. The stem cell extraction centrifugation extraction process as claimed in claim 5, wherein: the left side and the right side of the bottom end of the third rotating shaft (40) are fixedly connected with scraping pieces (42), the scraping pieces (42) are of bent structures, and the bottoms of the scraping pieces (42) are attached to the inner wall of the intermittent blanking shell (37).
7. The stem cell extraction centrifugation extraction process as claimed in claim 6, wherein: in step S3, the first rotating shaft (7) drives the fourth gear (28) to rotate through the third gear (10), and simultaneously drives the second rotating shaft (27) and the stirring blades (36) to rotate, and the stirring blades (36) stir the placenta fragments and the collagenase solution thrown into the intermittent blanking shell (37), so as to accelerate the digestion reaction speed of the placenta fragments and the collagenase solution.
8. The stem cell extraction centrifugation extraction process of claim 7, wherein: in step S3, the third gear (10) drives the third baffle (41) to rotate through the third rotating shaft (40), and when the third baffle is displaced above the blanking hole (39), the blanking hole (39) is closed, and at this time, the digestive juice formed by the placenta fragments and the collagenase solution cannot flow out of the blanking hole (39); the blanking holes (39) are gradually exposed along with the continuous rotation of the baffle (41), and at the moment, the digestive juice flows into the lower part of the intermittent blanking shell (37) from the blanking holes (39), and the circulation is carried out, so that the intermittent discharge is realized, and the placenta fragments and the collagenase solution can be fully digested and reacted in the intermittent blanking shell (37).
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