CN219363660U

CN219363660U - Lymphocyte culture device

Info

Publication number: CN219363660U
Application number: CN202223444867.5U
Authority: CN
Inventors: 张晗; 李立东
Original assignee: Beijing Taidong Biotechnology Co ltd
Current assignee: Beijing Taidong Biotechnology Co ltd
Priority date: 2022-12-22
Filing date: 2022-12-22
Publication date: 2023-07-18
Anticipated expiration: 2032-12-22

Abstract

The utility model discloses a lymphocyte culture device which comprises an upper box body, wherein the upper box body is fixedly arranged in the middle of the inside of a cell culture box supporting body, a lower box body is arranged at the lower end of the upper box body, a filter membrane is arranged between the upper box body and the lower box body, an upper bottle cap is arranged at the right end of the upper box body, a lower bottle cap is arranged at the right end of the lower box body, the cell culture box supporting body is electrically connected with an external controller, and a filter membrane between the upper box body and the lower box body is arranged, so that a cell culture medium with insufficient nutrition can be effectively and rapidly replaced, the cell damage is small, the culture environment of lymphocytes in a flowing state can be effectively realized, the growth environment of the lymphocytes in the body is more similar, meanwhile, the full acquisition of nutrient substances of the cells to the culture medium is facilitated, and the whole culture capacity is conveniently increased by the arrangement of the upper box body and the lower box body structure, and the large-capacity culture use is facilitated.

Description

Lymphocyte culture device

Technical Field

The utility model relates to the technical field of in-vitro maintenance of cell samples, in particular to a lymphocyte culture device.

Background

In recent years, research and development of cell therapy are rapid, and the in vitro maintenance culture of cells is an indispensable technical means in a plurality of fields including immune cell therapy, stem cell therapy and the like. The lymphocyte is used as a suspension cell from blood, the operation of in vitro maintenance and expansion culture of the cell is complex, and especially for large-scale lymphocyte culture, the traditional cell culture flask is not suitable for rapidly replacing a cell culture medium, and the volume of the culture flask and nutrient substances of the culture medium cannot be effectively utilized due to natural sedimentation of the cell. The conventional lymphocyte culture generally adopts a cell culture bottle of T25, T75 and T225, the separated and purified lymphocyte is added into the culture bottle containing the lymphocyte culture medium, under the condition that the nutrient of the culture medium is insufficient or even exhausted, the cell suspension is required to be taken out to a centrifuge tube, and the lymphocyte and the culture medium are separated by a centrifugal method, so that the aims of removing the nutrient-deficient culture medium and adding fresh culture medium are fulfilled.

At present, the technology has complicated process in the aspects of medium replacement and cell sample operation treatment, and the centrifugation process can increase cell damage, so that simple amplification treatment can not be carried out when the technology is used for transforming into large-scale industrialized cell culture. For uneven cell distribution and insufficient nutrient consumption of a culture medium caused by natural settlement of lymphocytes, no solution is adopted by adopting a traditional cell culture bottle and a traditional culture box at present, and a small number of special culture devices can solve the problem, but are generally complex and are not applicable to the traditional cell culture box.

Therefore, there is a need to propose a lymphocyte culture apparatus to solve the above technical problems.

Disclosure of Invention

The utility model aims to provide a lymphocyte culture device, which solves the problems that the prior device cannot efficiently culture lymphocytes with large capacity, is inconvenient to replace a cell culture medium and is not very convenient and efficient to use in the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a lymphocyte culture device, includes the upper box body, the upper box body is installed and is fixed in the middle of the inside of cell culture box support organism, the lower extreme of upper box body is provided with down the box body, be provided with the filter membrane between upper box body and the lower box body, the right-hand member of upper box body is provided with the bottle lid, the right-hand member of lower box body is provided with down the bottle lid, cell culture box support organism and external control ware electric connection.

The cell culture box support machine body comprises a driving motor, a concave shell, an inner sleeve cavity and a support fixing hole, wherein the inner sleeve cavity is formed in the concave shell, the support fixing holes are formed in the inner sides of the two ends of the concave shell, the driving motor is arranged on the outer side of the support fixing hole, and an output shaft of the driving motor is connected onto the upper box body through a coupling.

The upper box body comprises a culture sleeve cavity, a box body shell, an outer side supporting frame and an upper bottle opening, wherein the culture sleeve cavity is formed in the box body shell, the outer side supporting frame is arranged in the middle of two ends of the box body shell, the right side is provided with the upper bottle opening in the outer side of the outer side supporting frame, the upper bottle opening is connected to an upper bottle cover, the box body shell is sleeved on a cell culture box supporting body through the outer side supporting frame, and the right side is connected to the cell culture box supporting body.

The lower box body comprises a culture medium seepage port, a lower bottle opening, an inner culture cavity and a box body shell, wherein the inner culture cavity is formed in the box body shell, the culture medium seepage port is formed in the inner side of the upper end of the box body shell, the lower bottle opening is formed in the right end of the box body shell, the lower bottle opening is connected to a lower bottle cover, and the box body shell is connected to an upper box body.

The rotating diameter of the upper box body and the lower box body is smaller than the rotatable diameter of the inner side of the cell culture box supporting body.

Wherein, go up box body and last bottle lid through screw thread rotation fixed connection.

Wherein, lower box body and lower bottle lid pass through screw thread rotation fixed connection.

In summary, the beneficial effects of the utility model are as follows due to the adoption of the technology:

1. according to the utility model, the filter membrane between the upper box body and the lower box body is arranged, so that the cell culture medium with insufficient nutrition is effectively and rapidly replaced, the damage to cells is small, the culture environment of lymphocytes in a flowing state can be effectively realized, the condition that the lymphocytes are closer to the growth environment in vivo is effectively met, and meanwhile, the full acquisition of the nutrition substances of the cells to the culture medium is facilitated.

2. In the utility model, the upper box body and the lower box body are arranged, so that the whole culture capacity is conveniently increased, and the large-capacity culture is conveniently used.

Drawings

FIG. 1 is a schematic diagram showing a perspective structure of a lymphocyte culture apparatus according to the present utility model;

FIG. 2 is a schematic diagram showing the structure of a cell culture cassette support body of a lymphocyte culture apparatus according to the present utility model;

FIG. 3 is a schematic diagram showing the structure of an upper case of a lymphocyte culture apparatus according to the present utility model;

FIG. 4 is a schematic view of the structure of the lower case of a lymphocyte culture apparatus according to the present utility model.

In the figure: 1. an upper case body; 11. a culture sleeve cavity; 12. a box body shell; 13. an outer support; 14. an upper bottle mouth; 2. a filter membrane; 3. a bottle cap is arranged; 4. a lower bottle cap; 5. the cell culture box supports the body; 51. a driving motor; 52. a concave housing; 53. an internal jacket cavity; 54. a bracket fixing hole; 6. a lower case body; 61. medium seepage port; 62. a lower bottle mouth; 63. an inner culture chamber; 64. and a box body shell.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model. Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model.

Example 1

Referring to fig. 1 and 2, a lymphocyte culture apparatus, including upper box body 1, upper box body 1 installs and fixes in the middle of the inside of cell culture box support organism 5, the lower extreme of upper box body 1 is provided with down box body 6, be provided with filter membrane 2 between upper box body 1 and the lower box body 6, the right-hand member of upper box body 1 is provided with upper bottle lid 3, the right-hand member of lower box body 6 is provided with down bottle lid 4, cell culture box support organism 5 and external control ware electric connection, when using, through opening upper bottle lid 3 and lower bottle lid 4 on upper box body 1 and the lower box body 6 right-hand member, thereby be convenient for add lymphocyte culture and use, and whole in the culture, through filter membrane 2 between upper box body 1 and the lower box body 6, thereby the culture medium can effectively permeate, and the lymphocyte of culture can not pass through, be convenient for cultivate the separation use like this, and cell culture box support organism 5 is convenient for drive upper box body 1 and rotate, make the rotation rocking use of being convenient for.

In the utility model, the cell culture box supporting body 5 comprises a driving motor 51, a concave shell 52, an inner sleeve cavity 53 and a bracket fixing hole 54, the inner sleeve cavity 53 is arranged in the concave shell 52, the bracket fixing hole 54 is arranged at the inner sides of two ends of the concave shell 52, the driving motor 51 is arranged at the outer side of the right bracket fixing hole 54, an output shaft of the driving motor 51 is connected to the upper box body 1 through a coupling, the concave shell 52 of the cell culture box supporting body 5 is convenient for limiting placement and rotation use due to the area of the inner sleeve cavity 53, the upper box body 1 and the lower box body 6 are convenient for sleeve placement due to the area of the bracket fixing hole 54, and the output shaft of the driving motor 51 is connected to the upper box body 1 through the coupling, so that the driving motor 51 is convenient for stably driving the upper box body 1 to rotate and shake during rotation.

In the present utility model, the rotational diameter of the upper case 1 and the lower case 6 is smaller than the inside rotatable diameter of the cell culture cassette support body 5 so that no hindrance occurs at the time of rotation.

In the utility model, the upper box body 1 and the upper bottle cap 3 are fixedly connected through screw threads in a rotating way, so that the assembly, the disassembly and the use are convenient.

In the utility model, the lower box body 6 and the lower bottle cap 4 are fixedly connected through screw threads in a rotating way, so that the assembly, the disassembly and the use are convenient.

Working principle: when in use, the upper bottle cap 3 and the lower bottle cap 4 on the right ends of the upper box body 1 and the lower box body 6 are opened, so that lymphocyte culture is conveniently added, and the whole culture box is in culture, through the filter membrane 2 between the upper box body 1 and the lower box body 6, a culture medium can effectively permeate, and the cultured lymphocytes cannot pass through, so that the culture separation is convenient, the cell culture box support body 5 is convenient for driving the upper box body 1 to rotate, and the cell culture box is convenient for rotating and shaking.

Example two

Referring to fig. 1 and 3, in comparison with the first embodiment, the upper case 1 includes a culture chamber 11, a case housing 12, an outer support 13, and an upper bottle opening 14, the culture chamber 11 is provided in the case housing 12, the outer support 13 is provided in the middle of two ends of the case housing 12, the upper bottle opening 14 is provided in the outer side of the right outer support 13, the upper bottle opening 14 is connected to the upper bottle cap 3, the case housing 12 is sleeved on the cell culture case support body 5 through the outer support 13, and the right outer support 13 is connected to the cell culture case support body 5.

Working principle: the box body shell 12 of the upper box body 1 is connected to the cell culture box support body 5 in a sleeved mode through the right outer side support frame 13, so that the box body shell 12 can be used in a stable rotation mode, the box body shell 12 is used in a culture mode due to the region of the culture sleeve cavity 11, the culture is convenient, and the box body shell 12 is used in a culture mode through the upper bottle opening 14 at the right end, and the culture addition and the discharge are convenient.

Example III

Referring to fig. 1 and 4, in the present embodiment, compared with the second embodiment, the lower case 6 includes a culture medium permeation port 61, a lower bottle mouth 62, an inner culture cavity 63, and a case housing 64, the inner culture cavity 63 is provided in the case housing 64, the culture medium permeation port 61 is provided in the inner side of the upper end of the case housing 64, the lower bottle mouth 62 is provided in the right end of the case housing 64, the lower bottle mouth 62 is connected to the lower bottle cap 4, and the case housing 64 is connected to the upper case 1.

Working principle: the box body shell 64 of the lower box body 6 is connected to the upper box body 1, so that the upper box body 1 can conveniently drive the box body shell 64 to rotationally shake when in rotation, the box body shell 64 is convenient for culture and use due to the area of the inner culture cavity 63, and the box body shell 64 is convenient for permeation and use due to the culture medium permeation port 61 at the inner side of the upper end, and the box body shell 64 is convenient for culture and addition and discharge and use due to the lower bottle opening 62.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Claims

1. A lymphocyte culture apparatus, comprising an upper casing (1), characterized in that: the cell culture box comprises an upper box body (1), a cell culture box support body (5) and a cell culture box, wherein the upper box body (1) is fixedly arranged in the middle of the inside of the cell culture box support body (5), a lower box body (6) is arranged at the lower end of the upper box body (1), a filter membrane (2) is arranged between the upper box body (1) and the lower box body (6), an upper bottle cap (3) is arranged at the right end of the upper box body (1), a lower bottle cap (4) is arranged at the right end of the lower box body (6), and the cell culture box support body (5) is electrically connected with an external controller.

2. The lymphocyte culture apparatus according to claim 1, wherein: cell culture box supports organism (5) including driving motor (51), concave shell (52), inside cover chamber (53) and support fixed orifices (54), the inside of concave shell (52) is provided with inside cover chamber (53), the both ends inboard of concave shell (52) is provided with support fixed orifices (54), the right side the outside of support fixed orifices (54) is provided with driving motor (51), the output shaft of driving motor (51) passes through the coupling joint on last box body (1).

3. The lymphocyte culture apparatus according to claim 1, wherein: the upper box body (1) comprises a culture sleeve cavity (11), a box body shell (12), an outer side supporting frame (13) and an upper bottle opening (14), wherein the culture sleeve cavity (11) is arranged in the box body shell (12), the outer side supporting frame (13) is arranged in the middle of two ends of the box body shell (12), the outer side of the outer side supporting frame (13) is provided with the upper bottle opening (14), the upper bottle opening (14) is connected to the upper bottle cover (3), the box body shell (12) is sleeved on the cell culture box supporting body (5) through the outer side supporting frame (13), and the outer side supporting frame (13) is connected to the cell culture box supporting body (5).

4. The lymphocyte culture apparatus according to claim 1, wherein: lower box body (6) include culture medium oozes mouth (61), lower bottleneck (62), inboard cultivate chamber (63) and box body shell (64), the inside of box body shell (64) is provided with inboard cultivate chamber (63), the upper end inboard of box body shell (64) is provided with culture medium and oozes mouth (61), the right-hand member of box body shell (64) is provided with bottleneck (62) down, bottleneck (62) are connected on lower bottle lid (4) down, box body shell (64) are connected on last box body (1).

5. The lymphocyte culture apparatus according to claim 1, wherein: the rotating diameter of the upper box body (1) and the lower box body (6) is smaller than the inner rotatable diameter of the cell culture box supporting machine body (5).

6. The lymphocyte culture apparatus according to claim 1, wherein: the upper box body (1) and the upper bottle cap (3) are fixedly connected through screw threads in a rotating way.

7. The lymphocyte culture apparatus according to claim 1, wherein: the lower box body (6) and the lower bottle cap (4) are fixedly connected through screw threads in a rotating way.