CN105132277B - A kind of new separation of lymphocytes pipe - Google Patents
A kind of new separation of lymphocytes pipe Download PDFInfo
- Publication number
- CN105132277B CN105132277B CN201510628819.7A CN201510628819A CN105132277B CN 105132277 B CN105132277 B CN 105132277B CN 201510628819 A CN201510628819 A CN 201510628819A CN 105132277 B CN105132277 B CN 105132277B
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- China
- Prior art keywords
- plug
- unit
- cylindrical structure
- separation
- lymphocyte
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/05—Means for pre-treatment of biological substances by centrifugation
Abstract
The invention discloses a kind of new separation of lymphocytes pipe, including body, the body is tubular, and the bottom of tubular is centrum shape, a plug-in unit is provided with body, the plug-in unit is integrally formed with a concave structure by a cylindrical structure and formed, and the concave structure is located at the top of cylindrical structure, the bottom hollow out of cylindrical structure；Several cavernous structures for being used to separate lymphocyte are additionally provided with the plug-in unit.Lymphocyte separation medium is added below plug-in unit and then sample is added more than plug-in unit, it can directly make the layering of target lymphocyte above plug-in unit by centrifugation, by impurity separation below plug-in unit, take the direct can of lymphocyte pour out can, will not be spilt below plug-in unit.Its operation is simpler, and reduces impurity caused by error when pipette tips are drawn, purer than common separation.
Description
Technical field
The present invention relates to a kind of cell Separating tube, more particularly to a kind of separation of lymphocytes pipe, belong to bio-medical technology
Field.
Background technology
There was only common separation of lymphocytes pipe in the market, its method is by sample-adding product and lymphocyte separation medium
Centrifuged, can be layered in separating pipe after centrifugation, then experimenter needs to draw target lymphocyte with pipette tips.Its shortcoming is exactly
Easy suck up impurity in suction process, operation is cumbersome, not only wastes time and energy, and error is big.
The content of the invention
In order to solve the above technical problems, the present invention proposes new lymphocyte a kind of simple to operate and that separation is pure
Separating pipe.
In order to achieve the above object, technical scheme is as follows：The present invention devises a kind of unique plug-in application
In cell Separating tube, it can prevent density gradient centrifugation liquid and blood sample from mixing.The centre position of this plug-in unit
A center sorting hole is provided with, density gradient centrifugation liquid is added into bottom, sample edge by center sorting hole using pipette
The top that tube wall is added in plug-in unit, is thus added in without cautious above density gradient centrifugation liquid.Can be light after centrifugation
Pine pours into blood plasma and lymphocyte in new centrifuge tube, and impurity stays in plug-in unit bottom, saves the operating time and operation misses
Difference.Its concrete scheme is as follows：
A kind of new separation of lymphocytes pipe, including body, the body is tubular, and the bottom of tubular is centrum shape,
A plug-in unit is provided with body, the plug-in unit is integrally formed with a concave structure by a cylindrical structure and formed, described
Concave structure is located at the top of cylindrical structure, the bottom hollow out of cylindrical structure；It is additionally provided with the plug-in unit some
The individual cavernous structure for being used to separate lymphocyte.
Positioned at the center sorting hole that is provided centrally with of the concave structure, the center sorting hole it is a diameter of
1.8mm~2.3mm, lymphocyte separation medium are located at the lower section of plug-in unit, the pyramidal portion of body, are blood above plug-in unit, blood
Gone down in centrifugal process by center sorting hole, meanwhile, lymphocyte separation medium is expressed to plug-in unit from the cavernous structure of plug-in unit
Above, thus it is easy to come separation of lymphocytes.
Several U-type groove holes, the rounding in the U-type groove hole are also uniformly offered on the edge of the concave structure
Angular diameter is 0.8mm~1.2mm, and the opening in several U-type groove holes is outwardly, and the number in the U-type groove hole is three, when
When carrying out separation of lymphocytes, the blood sample above plug-in unit and lymph separating liquid is facilitated to carry out mass exchange.
Preferably, being provided with several reinforcements in the plug-in unit, the setting of reinforcement serves what is stablized and reinforce
Effect.
Preferably, the bottom of the cylindrical structure uniformly offers several arcuate sockets, the arc of the arcuate socket
Depth is 1mm, width 4mm, and the number of arcuate socket is three, arcuate socket to be provided for centrifuge tube lymph in centrifugation thin
Sample above born of the same parents' separating liquid and plug-in unit swaps.
Preferably, the diameter of the concave structure is bigger 1mm than the diameter of cylindrical structure, and the edge of concave structure is tight
The inwall of body is pasted, the diameter design of concave structure is bigger 1mm than the diameter of cylindrical structure, and its effect is such U-type groove hole
Aperture is also formed between body, when centrifugation, lymphocyte separation medium can be split into plug-in unit by these apertures
Above.
Preferably, the opening in the U-type groove hole is in contact with cylindrical structure, and form a waist-shaped hole.
Beneficial effects of the present invention：Lymphocyte separation medium is added below plug-in unit and then sample is added more than plug-in unit,
It can directly make the layering of target lymphocyte that by impurity separation below plug-in unit, it is thin to take lymph above plug-in unit by centrifugation
The direct can of born of the same parents is poured out, and will not be spilt below plug-in unit.Its operation is simpler, and reduces miscellaneous caused by error when pipette tips are drawn
Matter, it is purer than common separation.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structural representation of plug-in unit of the present invention；
Wherein：1. body, 2. plug-in units, 3. cylindrical structures, 4. concave structures, 5. center sorting holes, 6.U type grooves hole, 7.
Arcuate socket, 8. reinforcements.
Embodiment
With reference to embodiment, the present invention is further detailed explanation.
As depicted in figs. 1 and 2, a kind of new separation of lymphocytes pipe, including body 1, the body 1 are tubular, and cylinder
The bottom of shape is centrum shape, a plug-in unit 2 is provided with body 1, the plug-in unit 2 is by a cylindrical structure 3 and a concave surface
Structure 4 is integrally formed composition, and the concave structure 4 is located at the top of cylindrical structure 3, the bottom hollow out of cylindrical structure 3；Position
It is used to separate the cavernous structure of lymphocyte in being additionally provided with several on the plug-in unit 2.
Be provided centrally with a center sorting hole 5 positioned at the concave structure 4, the center sorting hole 5 it is a diameter of
1.8mm~2.3mm, the preferred 2mm of the present embodiment, if again should in the range of can, lymphocyte separation medium is located under plug-in unit 2
Side, the pyramidal portion of body 1, plug-in unit 2 are above blood, and blood is gone down in centrifugal process by center sorting hole 5, meanwhile,
The cavernous structure of lymphocyte separation medium from plug-in unit 2 is expressed to above plug-in unit 2, is thus easy to open separation of lymphocytes
Come.
Also uniformly offer several U-type groove holes 6 on the edge of the concave structure 4, the U-type groove hole, 6
A diameter of 0.8mm~the 1.2mm of rounding, preferred 1mm in the present embodiment, provided of course that within the range, can, and several
Outwardly, the number in the U-type groove hole 6 is three to the opening in U-type groove hole 6, convenient when separation of lymphocytes is being carried out
Blood sample and lymphocyte separation medium on plug-in unit 2 carry out mass exchange.
The bottom of the cylindrical structure 3 uniformly offers several arcuate sockets 7, and the arc depth of the arcuate socket 7 is
1mm, width 4mm, and the number of arcuate socket 7 be three, arcuate socket 7 be provided for centrifuge tube centrifugation when separating liquid and insert
Sample above part 2 swaps.
Several reinforcements 8 are provided with the plug-in unit 2, the setting of reinforcement 8 serves effect that is stable and reinforcing.
The diameter of the concave structure 4 is bigger 1mm than the diameter of cylindrical structure 3, and body is close at the edge of concave structure 4
Inwall, so, plug-in unit 2 would not easily be shifted over phenomenon in body 1, by the diameter design of concave structure 4 than circle
The big 1mm of diameter of column structure 3, its effect are also to form aperture between such U-type groove hole 6 and body 1, when centrifugation,
Lymphocyte separation medium can be split into above plug-in unit by these apertures.
The opening in the U-type groove hole 6 is in contact with cylindrical structure 3, and forms a waist-shaped hole.
Beneficial effects of the present invention：Lymphocyte separation medium is added below plug-in unit and then sample is added more than plug-in unit,
It can directly make the layering of target lymphocyte that by impurity separation below plug-in unit, it is thin to take lymph above plug-in unit by centrifugation
The direct can of born of the same parents pour out can, will not be spilt below plug-in unit.Its operation is simpler, and reduces when pipette tips are drawn caused by error
Impurity, purer than common separation, separation more rapidly, centrifuges 10 minutes；And centrifuge brake need not be closed.
Its centrifugal method is：
(1) in use, lymphocyte separation medium is added into the lower section of plug-in unit 2, the centrum portion of body 1 by center sorting hole
Point；
(2) then, blood sample is added from the edge of body 1, blood sample is located at the top of plug-in unit 2；
(3) and then centrifuged, above plug-in unit 2, impurity is separated below plug-in unit 2 for lymphocyte layering after centrifugation；
(4) when sampling by separating pipe in turn, center sorting hole is just on the lofty perch of plug-in unit 2, plus surface
The liquid in plug-in unit 2 inside the effect of tension force can not be poured out, and target substance above is poured out.Thus can be well by lymph
Cell is separated.
Claims (5)
1. a kind of new separation of lymphocytes pipe, including body (1), the body (1) is tubular, and the bottom of tubular is centrum
Shape, it is provided with a plug-in unit (2) in body (1), it is characterised in that the plug-in unit (2) is by a cylindrical structure (3) and one
Concave structure (4) is integrally formed composition, and the concave structure (4) is located at the top of cylindrical structure (3), cylindrical structure (3)
Bottom hollow out；Several cavernous structures for being used to separate lymphocyte, the poroid knot are additionally provided with the plug-in unit (2)
Structure is respectively to be uniformly arranged on several U-type groove holes (6) of concave structure (4) edge and be uniformly arranged on cylindrical structure
(3) several arcuate sockets (7) of bottom；The opening of the U-type groove hole (6) outwardly, a diameter of 0.8mm of its rounding~
1.2mm；The arc depth of the arcuate socket (7) is 1mm, width 4mm；And the diameter of concave structure (4) is than cylindrical structure (3)
The big 1mm of diameter, and the inwall of body (1) is close at the edge of concave structure (4)；Set positioned at the center of the concave structure (4)
It is equipped with a center sorting hole (5), a diameter of 1.8mm~2.3mm of the center sorting hole (5).
2. new separation of lymphocytes pipe according to claim 1, it is characterised in that the number of the U-type groove hole (6) is
Three.
3. new separation of lymphocytes pipe according to claim 2, it is characterised in that the number of the arcuate socket (7) is
Three.
4. new separation of lymphocytes pipe according to claim 2, it is characterised in that the opening of the U-type groove hole (6) with
Cylindrical structure (3) is in contact, and forms a waist-shaped hole.
5. new separation of lymphocytes pipe according to claim 1, it is characterised in that the plug-in unit (2) is if interior be provided with
Dry reinforcement (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510628819.7A CN105132277B (en) | 2015-09-29 | 2015-09-29 | A kind of new separation of lymphocytes pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510628819.7A CN105132277B (en) | 2015-09-29 | 2015-09-29 | A kind of new separation of lymphocytes pipe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105132277A CN105132277A (en) | 2015-12-09 |
| CN105132277B true CN105132277B (en) | 2018-03-09 |
Family
ID=54717853
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510628819.7A Active CN105132277B (en) | 2015-09-29 | 2015-09-29 | A kind of new separation of lymphocytes pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105132277B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110591901A (en) * | 2019-10-27 | 2019-12-20 | 李文强 | Cell separation tube |
| CN110702909A (en) * | 2019-10-31 | 2020-01-17 | 骏实生物科技(上海)有限公司 | Negative enrichment detection method for circulating tumor cells |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958776A (en) * | 2006-10-13 | 2007-05-09 | 深圳市达科为生物技术有限公司 | Separator tube for lymphocyte, method for separating lymphocyte in blood |
| CN202359118U (en) * | 2011-12-01 | 2012-08-01 | 石国清 | Gradient separation device of cast-off cells |
| CN104031831A (en) * | 2014-07-01 | 2014-09-10 | 北京圣浦博大生物科技有限公司 | Blood cell separation tube and separation method of mononuclear cell |
| CN204022830U (en) * | 2014-08-19 | 2014-12-17 | 任峰 | A kind of cell Separating tube for separating of extracting PBMC |
| CN104762196A (en) * | 2015-04-14 | 2015-07-08 | 深圳市达科为生物工程有限公司 | Cell separation tube |
| CN204981871U (en) * | 2015-09-29 | 2016-01-20 | 杭州科鼎生物科技有限公司 | Novel lymphocyte separator tube |
-
2015
- 2015-09-29 CN CN201510628819.7A patent/CN105132277B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958776A (en) * | 2006-10-13 | 2007-05-09 | 深圳市达科为生物技术有限公司 | Separator tube for lymphocyte, method for separating lymphocyte in blood |
| CN202359118U (en) * | 2011-12-01 | 2012-08-01 | 石国清 | Gradient separation device of cast-off cells |
| CN104031831A (en) * | 2014-07-01 | 2014-09-10 | 北京圣浦博大生物科技有限公司 | Blood cell separation tube and separation method of mononuclear cell |
| CN204022830U (en) * | 2014-08-19 | 2014-12-17 | 任峰 | A kind of cell Separating tube for separating of extracting PBMC |
| CN104762196A (en) * | 2015-04-14 | 2015-07-08 | 深圳市达科为生物工程有限公司 | Cell separation tube |
| CN204981871U (en) * | 2015-09-29 | 2016-01-20 | 杭州科鼎生物科技有限公司 | Novel lymphocyte separator tube |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105132277A (en) | 2015-12-09 |
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Effective date of registration: 20191209 Address after: 310051 No.88 Jiangling Road, Xixing street, Binjiang District, Hangzhou City, Zhejiang Province Patentee after: Zhejiang Baidi Biotechnology Co., Ltd Address before: 310000 Zhejiang District of Xihu District city of Hangzhou province Shengyuan gudun road 6-503 Patentee before: HANGZHOU BIOCODE TECHNOLOGY CO., LTD. |