CN106967590A

CN106967590A - Big specification cell Separating tube

Info

Publication number: CN106967590A
Application number: CN201610021645.2A
Authority: CN
Inventors: 李小阁
Original assignee: Individual
Current assignee: Li Wenqiang
Priority date: 2016-01-14
Filing date: 2016-01-14
Publication date: 2017-07-21

Abstract

The present invention announces big specification cell Separating tube, is mainly used in the separation of 40 ~ 150ml volume cells samples, client is when in use, easy to operate, effectively improves separative efficiency, cell purity and cell recoveries.

Description

Big specification cell Separating tube

Technical field

In biology and chemical field, often according to the density size of different solid particles in sample, the separation of heterogeneity in sample is realized, is mainly used in the separation of different cells in biology.

Background technology

With continuing to develop for cell biology, it is continuous ripe in particular with the application of biological therapy technology, cell separation turns into technological means the most frequently used in every RESEARCH ON CELL-BIOLOGY and clinical practice, cell is separated using density gradient method, due to good separating effect, method is simple, and cost is low, is widely used.It is the density variation by sample rate separating liquid, sample cell suspension and various cells, by the effect of centrifugal force, different cells is formed different layerings in separating pipe.

With continuing to develop for technology, Cord blood separation storage, the storage of healthy cell, more and more paid attention to, biological therapy field also has more client to need to separate more cell sample, bothered before with 50ml centrifuge tubes lock out operation, cost is high, many clients are directly using 225ml or 250ml centrifuge tubes separation cell, but when using 225ml or 250ml centrifuge tubes separation cell, liquid level contact area is than 50ml or 15ml separating pipes bigger much times, sample and separating liquid are more prone to mixing, more and more higher is required to sample-adding, when drawing tunica albuginea confluent monolayer cells, cell recoveries are lower.

The problem of present invention exists for more than, develops a kind of new separating pipe, and separating pipe sample-adding is convenient, effectively lowers the mixing of sample and separating liquid, while can effectively lift the purity and the rate of recovery of cell again, lifts separative efficiency.

The content of the invention

The invention provides a kind of big specification cell Separating tube, including centrifuge tube and distributor two parts composition, separating pipe sample-adding is convenient, effectively lowers the mixing of sample and separating liquid, while can effectively lift the purity and the rate of recovery of cell again, lifts separative efficiency.

The big specification cell Separating tube, described centrifuge tube includes two kinds of conical bottom centrifuge tubes of 225ml and 250ml specifications.

The big specification cell Separating tube, the distributor can enter centrifuge the mouth of pipe, in that context it may be convenient to be fixed in centrifuge tube with corresponding centrifuge tube dimensional fits, difference.

The big specification separating pipe, affiliated distributor, top is tubular structure, and tubular structure side wall is provided with aperture, and sample flows out through aperture, is laid on separating liquid liquid level.

The big specification separating pipe, affiliated distributor, lower end is a leg, branch has a comb shape dividing plate, the impulsive force of buffered samples in the middle of conical bottom center, leg and tubular structure, separating liquid liquid level is just under comb shape dividing plate, and during sample-adding, sample can be gently laid on separating liquid liquid level.

The big specification separating pipe, affiliated distributor, the tubular structure, taper end only has the perforate of bottom one below, when drawing tunica albuginea layer with 25ml serum pipettes, liquid relief pipe outer wall is just blocked up on aperture, makes stratified liquid orderly from bottom aperture sucks serum tube successively, it is prevented effectively from and upsets each layering, so that cell purity and the quantity lifting drawn.

Brief description of the drawings

The big specification separating pipe schematic diagrames of Fig. 1

Fig. 2 dispensing arrangements 3D schemes

Fig. 3 comb shape dividing plates 3D schemes

Each mark is as follows in figure：1 centrifuge tube；2 distributor tubular structures；3 distributor legs；4 side openings；5 comb shape dividing plates, 6 centrifugation lids, 7 separating liquids, 8 conical structures, 9 comb shape dividing plate teeth.

Embodiment

The composition of the big specification cell Separating tube of embodiment 1

Conventional 225ml centrifuge tubes or 250ml centrifuge tubes（As shown in mark 1 in Figure of description）, it is small-bore, abdominous conical centrifuge tube, centrifuge tube bore is generally between 24 ~ 30mm, maximum outside diameter is between 50 ~ 65mm, centrifuge tube is generally PP apyrogeneity sterile products.Distributor is medical PP by centrifuging mouth of pipe insertion tube bottom, distributor, PC or PS materials are constituted, by centrifuging the supporting role of the mouth of pipe and centrifuge tube bottom, are fixed in centrifuge tube.

The separation process of embodiment 2

According to the length of the leg of distributor, add a certain amount of separating liquid, typically there are 60ml, 80ml, 100ml, several specifications of 120ml, average separates the anticoagulation of 1 ~ 2 times of volume per ml separating liquids, and separating liquid liquid level keeps concordant with comb type dividing plate lower edge, is then poured slowly into fresh anticoagulation, aperture in slope in the allocated device pipe of blood stream, bottom sidewall（Mark 4 in accompanying drawing）, by comb shape dividing plate（Mark 5 in accompanying drawing）, triple buffer slows down, is uniformly laid on separating liquid, it is to avoid the mixing of blood and separating liquid.

After centrifugation, red blood cell is located at the bottom, and separating liquid, tunica albuginea layer are followed successively by upwards（Mononuclear cell layer）, plasma layer.Comb shape dividing plate is located under tunica albuginea layer, now, and distributor bottommost is inserted with 25ml serum tubes, then liquid passes sequentially through bottom side opening（Mark 4 in Fig. 1, Fig. 2）Into distributor, suck successively in pipette, it is ensured that liquid layer is not disturbed, so as to obtain more, the higher target cell of purity, realization is efficiently separated.

Claims

1. a kind of big specification cell Separating tube, the product is made up of centrifuge tube and distributor two parts, distributor is applied in combination with centrifuge tube, is effectively reduced the mixing of sample and separating liquid, improves the recovery quantity of cell.

2. the big specification cell Separating tube described in claim 1, the centrifuge tube includes two kinds of specifications, respectively 225ml specifications, centrifuge tube body outer diameter is 50mm ~ 65mm；250ml specifications, centrifuge tube body outer diameter is 50mm ~ 65mm.

3. the big specification cell Separating tube described in claim 1, the distributor, by tubular structure, comb shape dividing plate, the part of leg 3 composition, three parts are connected as a single entity, and tubular structure and leg length ratio is between 0.5 ~ 3.

4. the big specification cell Separating tube described in claim 3, the distributor, the tubular structure, it is characterised in that tubular structure on comb shape dividing plate junction and upper portion side wall with having gap, and sample enters centrifuge tube by gap.

5. the big specification cell Separating tube described in claim 3, the distributor, the tubular structure, it is characterised in that the difference of the tubular structure upper end diameter 5 ~ 22mm bigger than bottom diameter, its upper end external diameter and centrifuge tube spout diameter is positive and negative 5mm.

6. the big specification cell Separating tube described in claim 3, the distributor, the comb shape dividing plate, its dividing plate is circle, center is conical structure, during each tooth top half of comb shape dividing plate is double inclined surface structure, centrifugal process, the larger cell of proportion can be slipped under dividing plate by circular cone or inclined-plane, to prevent from stopping sinking cell.