CN205313547U - Centrifuging tube suitable for a single karyocyte layering - Google Patents
Centrifuging tube suitable for a single karyocyte layering Download PDFInfo
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- CN205313547U CN205313547U CN201521129673.3U CN201521129673U CN205313547U CN 205313547 U CN205313547 U CN 205313547U CN 201521129673 U CN201521129673 U CN 201521129673U CN 205313547 U CN205313547 U CN 205313547U
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Abstract
The utility model discloses a centrifuging tube suitable for a single karyocyte layering, including tube cap, upper portion pipe shaft, middle part pipe shaft and the lower part pipe shaft that from top to bottom sets gradually, the diameter of upper portion pipe shaft from top to bottom diminishes gradually, and its lower part is with 150 circular arc angle downwardly extending in order to form the low tube interface, the mid portion of middle part pipe shaft is hollow out construction, hose in the middle of being provided with in the hollow out construction, the upper portion of middle hose with the low tube interface connection of upper portion pipe shaft, the lower part of middle hose with the top tube interface connection of lower part pipe shaft, wherein, the lower part pipe shaft is tapered flat structure. The utility model discloses a centrifuging tube is when the centrifugation, and tunica albuginea layer and plasma layer can be floated on hose upper strata, and parting liquid and red blood cell layer can be depositd in hose lower floor, only need clip the hose with the hemostatic forceps during separation, pour tunica albuginea layer and plasma layer liquid can, can ensure to exhaust completely tunica albuginea layer cell like this and can not sneak into the red blood cell again.
Description
Technical field
The utility model relates to a kind of centrifuge tube being applicable to single core cell layering.
Background technology
At present, peripheral blood mononuclear cell (Peripheralbloodmononuclearcell, PBMC) i.e. periphery blood has the cell of single core, comprises lymphocyte and monocyte. First vitro detection lymphocyte wants separating peripheral blood mononuclear cells, separation method main at present is Ficoll-hypaque (dextran-urografic acid methylglucamine salt) density gradient centrifugation, because the proportion of each formed elements exists difference in blood, therefore it is separated. Red corpuscle and granulocyte density are greater than layering liquid, are aggregated into string money shape because red corpuscle runs into Ficoll simultaneously and are deposited at the bottom of pipe. Thrombocyte is then suspended in because density is little in blood plasma, has the suitable single core cell of the liquid-tight degree with layering only intensive in the interface of plasma layer and layering liquid, in tunica albuginea shape, draws this confluent monolayer cells and pass through washing centrifugal resuspended. This method separation single core cell purity can reach 95%, and lymphocyte accounts for 90%～95%, and cell acquisition rate can reach more than 80%.
But in actual mechanical process, the absorption of tunica albuginea layer is the work testing very much experiment operator experience, need careful being inserted by suction pipe above single core cell tunica albuginea layer, draw this layer of single core cell along test tube wall periphery, avoid being drawn to the parting liquid below tunica albuginea layer as far as possible. This operation steps needs experiment operator to have comparatively skilled experimental operating skill, not so can have influence on the collecting amount of single core cell. In order to address this problem, we devise a centrifuge tube being specifically designed to single core cell density gradient centrifugation.
Practical novel content
The utility model object is that the defect existed for prior art provides a kind of centrifuge tube being applicable to single core cell layering.
The utility model for achieving the above object, adopts following technical scheme: a kind of centrifuge tube being applicable to single core cell layering, comprises the pipe lid, top pipe shaft, middle part pipe shaft and the bottom pipe shaft that arrange successively from top to bottom;The diameter of described top pipe shaft from top to bottom diminishes gradually, and its underpart extends to be formed lower interface tube downwards with the arc angle of 150 °; The middle portion of described middle part pipe shaft is engraved structure, is provided with intermediate hose in described engraved structure, and the top of described intermediate hose is connected with the lower interface tube of described top pipe shaft, and the bottom of described intermediate hose is connected with the upper interface tube of described bottom pipe shaft; Wherein, described bottom pipe shaft is tapered flat-bottom structure.
Further, described pipe lid adopts plastic material to make, and its diameter is 3.4cm, and height is 1.2cm, and thick is 0.15cm.
Further, described pipe lid is threaded with described top pipe shaft, and the outside of described pipe lid is provided with an anti-skidding vertical line of circle.
Further, described top pipe shaft adopts plastic material to make, and its length is 53cm, and its upper diameter is 29cm, and its underpart diameter is 23.6cm.
Further, the length of the lower interface tube of described top pipe shaft is 6cm, and diameter is 0.85cm.
Further, described intermediate hose adopts transparent rubber material to make.
Further, the length of described bottom pipe shaft is 43cm.
The beneficial effects of the utility model: centrifuge tube of the present utility model is when centrifugal, tunica albuginea layer and plasma layer can float over flexible pipe upper strata, parting liquid and red corpuscle layer can be deposited in flexible pipe lower floor, only need during separation to clamp flexible pipe with mosquito forceps, pour out the liquid of tunica albuginea layer and plasma layer, can guarantee that exhausting tunica albuginea confluent monolayer cells completely can not be mixed into again red corpuscle like this.
Accompanying drawing explanation
Fig. 1 structural representation of the present utility model.
Embodiment
Shown in Fig. 1, it relates to a kind of centrifuge tube being applicable to single core cell layering, comprise the pipe lid 1, top pipe shaft 2, middle part pipe shaft 3 and the bottom pipe shaft 4 that arrange successively from top to bottom; The diameter of described top pipe shaft 2 from top to bottom diminishes gradually, and its underpart extends to be formed lower interface tube downwards with the arc angle of 150 °; The middle portion of described middle part pipe shaft 3 is engraved structure, intermediate hose 31 it is provided with in described engraved structure, the top of described intermediate hose 31 is connected with the lower interface tube of described top pipe shaft 2, and the bottom of described intermediate hose 31 is connected with the upper interface tube of described bottom pipe shaft 4; Wherein, described bottom pipe shaft 4 is in tapered flat-bottom structure.
During specific design, described pipe lid 1 adopts plastic material to make, and its diameter is 3.4cm, and height is 1.2cm, and thick is 0.15cm. Described pipe lid 1 is threaded with described top pipe shaft 2, and the outside of described pipe lid 1 is provided with an anti-skidding vertical line of circle. Described top pipe shaft 2 adopts plastic material to make, and its length is 53cm, and its upper diameter is 29cm, and its underpart diameter is 23.6cm. The length of the lower interface tube of described top pipe shaft 2 is 6cm, and diameter is 0.85cm. Described intermediate hose 31 adopts transparent rubber material to make. The length of described bottom pipe shaft 4 is 43cm.
After tested, when the pore size of interface tube is set to 0.85cm instantly, when test tube can be made to topple over, the surface tension of liquid that Ficoll surface produces can guarantee that the red corpuscle layer bottom test tube can not be poured out.
The total volume of this centrifuge tube of the present utility model is 40ml, is applicable to 15ml-20ml blood separation single core cell.
Experimental procedure:
1, in centrifuge tube, add 20ml lymphocyte separation medium (being just positioned at below rubber hose), slowly add 20ml blood, it is ensured that liquid level layering.
2, carefully centrifuge tube is put into whizzer 400g centrifugal 30 minutes.
3, after centrifugal layering, it is seen that orlop is red corpuscle and granulocyte (about 5-8ml) last layer is 20ml lymphocyte separation medium, and flexible pipe top is divided into part lymphocyte separation medium, tunica albuginea layer, blood plasma.
4, get vascular clamp and clamp flexible pipe, directly centrifuge tube is vertically poured out the liquid on flexible pipe upper strata, whole single core cells can be obtained easily and fast.
Time centrifugal, tunica albuginea layer and plasma layer can float over flexible pipe upper strata, and parting liquid and red corpuscle layer can be deposited in flexible pipe lower floor, only need to clamp flexible pipe with mosquito forceps during separation, the liquid pouring out tunica albuginea layer and plasma layer is just passable, can guarantee that exhausting tunica albuginea confluent monolayer cells completely can not be mixed into again red corpuscle like this.
The foregoing is only better embodiment of the present utility model, not in order to limit the utility model, all within spirit of the present utility model and principle, any amendment of doing, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (7)
1. one kind is applicable to the centrifuge tube of single core cell layering, it is characterised in that, comprise the pipe lid (1), top pipe shaft (2), middle part pipe shaft (3) and the bottom pipe shaft (4) that arrange successively from top to bottom; The diameter on described top pipe shaft (2) from top to bottom diminishes gradually, and its underpart extends to be formed lower interface tube downwards with the arc angle of 150 °; The middle portion at described middle part pipe shaft (3) is engraved structure, intermediate hose (31) it is provided with in described engraved structure, the top of described intermediate hose (31) is connected with the lower interface tube on described top pipe shaft (2), and the bottom of described intermediate hose (31) is connected with the upper interface tube of described bottom pipe shaft (4); Wherein, described bottom pipe shaft (4) is in tapered flat-bottom structure.
2. a kind of centrifuge tube being applicable to single core cell layering as claimed in claim 1, it is characterised in that, described pipe lid (1) adopts plastic material to make, and its diameter is 3.4cm, and height is 1.2cm, and thick is 0.15cm.
3. a kind of centrifuge tube being applicable to single core cell layering as claimed in claim 2, it is characterized in that, described pipe lid (1) is threaded with described top pipe shaft (2), and the outside of described pipe lid (1) is provided with an anti-skidding vertical line of circle.
4. a kind of centrifuge tube being applicable to single core cell layering as claimed in claim 1, it is characterised in that, described top pipe shaft (2) adopts plastic material to make, and its length is 53cm, and its upper diameter is 29cm, and its underpart diameter is 23.6cm.
5. a kind of centrifuge tube being applicable to single core cell layering as claimed in claim 1, it is characterised in that, the length of the lower interface tube on described top pipe shaft (2) is 6cm, and diameter is 0.85cm.
6. a kind of centrifuge tube being applicable to single core cell layering as claimed in claim 1, it is characterised in that, described intermediate hose (31) adopts transparent rubber material to make.
7. a kind of centrifuge tube being applicable to single core cell layering as claimed in claim 1, it is characterised in that, the length of described bottom pipe shaft (4) is 43cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521129673.3U CN205313547U (en) | 2015-12-30 | 2015-12-30 | Centrifuging tube suitable for a single karyocyte layering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521129673.3U CN205313547U (en) | 2015-12-30 | 2015-12-30 | Centrifuging tube suitable for a single karyocyte layering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205313547U true CN205313547U (en) | 2016-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521129673.3U Active CN205313547U (en) | 2015-12-30 | 2015-12-30 | Centrifuging tube suitable for a single karyocyte layering |
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| Country | Link |
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| CN (1) | CN205313547U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929399A (en) * | 2015-12-30 | 2017-07-07 | 协和华东干细胞基因工程有限公司 | A kind of centrifuge tube suitable for mononuclearcell layering |
| CN113440927A (en) * | 2020-03-27 | 2021-09-28 | 张文杰 | Device and method for extracting water-soluble components of emulsified and broom-corn-like fat |
-
2015
- 2015-12-30 CN CN201521129673.3U patent/CN205313547U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929399A (en) * | 2015-12-30 | 2017-07-07 | 协和华东干细胞基因工程有限公司 | A kind of centrifuge tube suitable for mononuclearcell layering |
| CN106929399B (en) * | 2015-12-30 | 2020-03-31 | 协和华东干细胞基因工程有限公司 | Centrifuging tube suitable for mononuclear cell layering |
| CN113440927A (en) * | 2020-03-27 | 2021-09-28 | 张文杰 | Device and method for extracting water-soluble components of emulsified and broom-corn-like fat |
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