CN211339495U

CN211339495U - Separation device for separating PBMC (peripheral blood mononuclear cell)

Info

Publication number: CN211339495U
Application number: CN201922149148.2U
Authority: CN
Inventors: 刘洋洋; 崔恒宓
Original assignee: Jiangsu Yinuo Biomedical Research Institute Co ltd
Current assignee: Jiangsu Yinuo Biomedical Research Institute Co ltd
Priority date: 2019-12-04
Filing date: 2019-12-04
Publication date: 2020-08-25
Anticipated expiration: 2029-12-04

Abstract

The utility model discloses a separator for separating Peripheral Blood Mononuclear Cell (PBMC) cell, include the centrifuging tube and be located the inside separator of centrifuging tube, separator includes hollow supporting part and is located the inside separating layer of supporting part, definition the upper end of supporting part has relative A end and B end, the separating layer by the A end to the below slope of B end, the separating layer will the supporting part is separated into about the space proportion and is done, the separating layer is close to the separation hole has been seted up to one side of B end. The utility model discloses simple structure, convenient to use, and low in production cost are suitable for using widely.

Description

Separation device for separating PBMC (peripheral blood mononuclear cell)

Technical Field

The utility model belongs to the technical field of biological assay equipment, especially, relate to a cell separation device.

Background

Peripheral Blood Mononuclear Cells (PBMC) are predominantly an immunologically relevant cell population in Peripheral blood. Has important roles in basic research and some clinical cell therapies. The rapid separation of high purity PMBC cells from peripheral blood is a very important technical approach. The Ficoll density gradient centrifugation is conventionally used for separating cells, and when a separated density gradient layer is finally obtained, the PBMC cells on the upper layer need to be carefully sucked or poured, the red blood cell pollution on the lower layer is prevented, and the operation needs to be carefully carried out.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the main technical problem who solves provides a separator for separating PBMC cell, simple structure, convenient to use, and low in production cost is suitable for and uses widely.

In order to solve the technical problem, the utility model discloses a technical scheme be: the utility model provides a separator for separating PBMC cell, includes the centrifuging tube and is located the inside separator of centrifuging tube, separator includes hollow supporting part and is located the inside separating layer of supporting part, the definition the upper end of supporting part has relative A end and B end, the separating layer by the A end to the below slope of B end, the separating layer will the interior partition of centrifuging tube is the space ratio about 2:1, the separating hole has been seted up to one side that the separating layer is close to the B end.

Further, the separation layer and the upper end of the support portion constitute an eccentric cone.

Further, the inclination angle of the separation layer is 20 °.

Further, the outer wall of the supporting part is matched with the inner wall of the centrifugal tube.

Further, the lower end of the support portion matches the upper edge of the conical structure below the centrifuge tube.

Further, the separation layer is an impermeable polystyrene plastic film.

Further, the diameter of the separation hole is 5 mm.

Further, the centrifuge tube is 50 ml.

Further, the supporting part is a hollow cylinder.

Further, the separation layer is an elliptical separation layer.

The beneficial effects of the utility model are that following several points have at least:

1. the separation device for separating peripheral blood mononuclear cells of the utility model has convenient and simple operation, can realize the shortening of the separation time, and can quickly extract PBMC with low red blood cell pollution; the separating piece can be directly placed in a 50ml centrifugal tube which is conventionally used in a laboratory, and an additional centrifugal tube is not needed;

2. the separation device of the utility model adopts the inclined separation interface with the opening at the side edge, so as to prevent the blood layer from being mixed with the separation layer and avoid the need of closing the brake of the centrifuge during centrifugation; the isolated PMBC cells can be rapidly poured from one side into a centrifuge tube directly after centrifugation without concern for contaminating the isolated PBMC cells with a backflow of blood cells.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic structural view of the separating member of the present invention;

the parts in the drawings are marked as follows:

centrifuge tube 1, separator 2, support 21, separation layer 22, separation hole 23 and inclination angle α.

Detailed Description

The following detailed description of the preferred embodiments of the present invention will be provided in conjunction with the accompanying drawings, so as to enable those skilled in the art to more easily understand the advantages and features of the present invention, and thereby define the scope of the invention more clearly and clearly.

Example (b): a separation device for separating PBMC cells is shown in figures 1-2 and comprises a centrifuge tube 1 and a separation part 2 positioned in the centrifuge tube, wherein the separation part comprises a hollow support part 21 and a separation layer 22 positioned in the support part, the upper end of the support part is defined to be provided with an opposite A end and an opposite B end, the separation layer inclines from the A end to the lower part of the B end, the separation layer divides the interior of the centrifuge tube into an upper space and a lower space in a ratio of 2:1, and one side, close to the B end, of the separation layer is provided with a separation hole 23.

The separating layer and the upper end of the supporting part form an eccentric cone.

The angle of inclination alpha of the separating layer is 20 degrees.

The outer wall of the supporting part is matched with the inner wall of the centrifugal tube.

The lower extreme of supporting part with the upper edge phase-match of the toper structure of the below of centrifuging tube.

The separation layer is an impermeable polystyrene plastic film.

The diameter of the separation hole is 5 mm.

The centrifuge tube is 50 ml.

The supporting part is a hollow cylinder.

The separation layer is an elliptical separation layer.

The utility model discloses a use method as follows:

(1) in the biological safety cabinet, the separating piece is placed in a 50ml centrifugal tube, and the centrifugal tube is inclined to ensure that the separating hole of the separating layer is arranged above; the cell separation solution was injected into the lower layer of the separator through the separation hole using a syringe. The tube was tilted so that the separation well was above, the lower layer of the separator was filled with cell separation fluid, and excess air was vented. When the centrifugal tube is vertically placed, the cell separation solution only needs to be submerged in the separation hole; adding the whole blood diluted by PBS 1:1 into a centrifuge tube along the tube wall;

(2) after a centrifugal tube cover is covered, a centrifugal machine is used for separating by 1000g centrifugal force for 10 min; after separation, significant stratification occurred within the centrifuge tubes: the red blood cells enter the lower part of the separation (the bottom of the centrifuge tube), and the red blood cells, the separation liquid (the isolation layer), the PBMC cell layer and the diluted blood plasma are arranged upwards in sequence;

(3) after centrifugation, the centrifuge tube is dumped along the opposite side of the separation well, PBMC and serum and a small portion of the centrate will be easily discharged from the centrifuge tube, and all red blood cells and most of the cell centrate are trapped under the spacer layer.

The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structure changes made in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the same principle as the present invention.

Claims

1. A separation device for separating PBMC cells, characterized by: including centrifuging tube and being located the inside separator of centrifuging tube, separator includes hollow supporting part and is located the inside separation layer of supporting part, the definition the upper end of supporting part has relative A end and B end, the separation layer by the A end to the below slope of B end, the separation layer will the interior partition of centrifuging tube becomes upper and lower space ratio and is 2:1, the separation hole has been seted up to one side that the separation layer is close to the B end.

2. An isolation device for isolating PBMC cells according to claim 1, characterized in that: the separating layer and the upper end of the supporting part form an eccentric cone.

3. An isolation device for isolating PBMC cells according to claim 2, characterized in that: the inclination angle of the separation layer is 20 degrees.

4. An isolation device for isolating PBMC cells according to claim 1, characterized in that: the outer wall of the supporting part is matched with the inner wall of the centrifugal tube.

5. An isolation device for isolating PBMC cells according to claim 1, characterized in that: the lower extreme of supporting part with the upper edge phase-match of the toper structure of the below of centrifuging tube.

6. An isolation device for isolating PBMC cells according to claim 1, characterized in that: the separation layer is an impermeable polystyrene plastic film.

7. An isolation device for isolating PBMC cells according to claim 1, characterized in that: the diameter of the separation hole is 5 mm.

8. An isolation device for isolating PBMC cells according to claim 1, characterized in that: the centrifuge tube is 50 ml.

9. An isolation device for isolating PBMC cells according to claim 1, characterized in that: the supporting part is a hollow cylinder.

10. An isolation device for isolating PBMC cells according to claim 1, characterized in that: the separation layer is an elliptical separation layer.