CN105132278B - Cell Separating tube with sieve plate separation and Extraction PBMC - Google Patents
Cell Separating tube with sieve plate separation and Extraction PBMC Download PDFInfo
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- CN105132278B CN105132278B CN201510664306.1A CN201510664306A CN105132278B CN 105132278 B CN105132278 B CN 105132278B CN 201510664306 A CN201510664306 A CN 201510664306A CN 105132278 B CN105132278 B CN 105132278B
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- sieve plate
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- isolation sieve
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/05—Means for pre-treatment of biological substances by centrifugation
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Abstract
The present invention relates to a kind of cell Separating tube with sieve plate separation and Extraction PBMC, the separating pipe includes lid, body, isolation sieve plate and separating liquid, the lid coaxial threaded engagement sealing shroud is loaded on the top of body, bottom in the body isolates sieve plate with the installation of inboard wall of tube body co-axial seal, uniform interval is formed with several sieve apertures on isolation sieve plate, the body inner bottom part encapsulation cell separating liquid below the isolation sieve plate.Blood sample quickly can be added to above isolation sieve plate by the isolation sieve plate of this separating pipe, and it can prevent blood preparation from being mixed with the separating liquid below sieve plate, and, isolation sieve plate also efficiently avoid be infused in isolation sieve plate below separating liquid separating pipe be inverted, transport jolt vibrations or collision situation under, " reflux " arrives the top of the isolation sieve plate of separating pipe, ensure that cell separating liquid is constantly in the separating pipe of the lower section of isolation sieve plate, so that cell extraction operation is more reliable, conveniently, transport also more convenient.
Description
Technical field
The invention belongs to medical technology class technical field of medical instruments, is related to the apparatus that cell separates, especially a kind of band
Sieve plate separation and Extraction PBMC cell Separating tube.
Background technology
It is thin that PMBC (Periphearal blood mononuclear cell, PBMC) is primarily referred to as lymph
Born of the same parents, it is the most commonly used cell mass of immunological experiment.In basis and clinical in vitro study, monocyte is more conventional
A kind of cell.How the higher monocyte of purity is quickly and efficiently isolated from peripheral blood, be always medicine technology field
The problem constantly inquired into.The method of traditional separation monocyte is Ficoll density gradient separations, but density level bands
The difficult point of degree layering is：In sample-adding and centrifugal process, if the too fast, centrifuge tube of operator's sample-adding is uneven or centrifuge in height
The lower instantaneous brake of speed rotation, can all cause the layering of monocyte and separating liquid unintelligible, can all influence separating experiment effect.For
Solves this problem, " (patent publication No. is a kind of cell Separating tube for separation and Extraction PBMC for existing patent：
CN204022830U) " can solve this problem, the cell Separating tube carries funnel dividing plate.Because PBMC cell Separating tube is
Needing to fill could be as overall medical equipment after quantitative cell separating liquid, there is provided what medical science used.With funnel every
The PBMC cell Separating tubes of plate have been injected into the cell separation in the lower partition in the case of transport vibrations, jolting or be inverted
Liquid is possibly through " funnel hole " the anti-first half for being flowed into dividing plate.The blood that so experiment operator cannot separate needs
Liquid sample is directly injected into separating pipe, it is necessary to the separating pipe for such situation occur first also is carried out a centrifugal process, made
Obtaining cell separating liquid, all " recovery " to the bottom of separating pipe, can continue to carry out by operating process, not only waste substantial amounts of
Human and material resources, operate also very cumbersome, great inconvenience is brought to user.
By retrieval, such as the next chapter patent publication us related to present patent application is found：
A kind of cell Separating tube (CN204022830U) for separation and Extraction PBMC, it is related to one kind and is used for separation and Extraction
PBMC cell Separating tube, including lid and body, the lid coaxial threaded cover the top for being installed on body, the body
Interior middle and lower part co-axial seal installs a separation layer, and the separation layer is open-topped hollow funnel-form, the separation layer
The tapered bottom in bottom, the bottom centre of the separation layer are coaxially disposed a liquid injection hole.This cell Separating tube is due to separation layer
Blood sample, rapidly can be added on the liquid level of separating liquid by design, and can anti-Hemostatic Oral Liquid layer and separating liquid mixing, from
During the heart, without closing centrifuge brake；Directly the PBMCs isolated can be poured into easily in new centrifuge tube after centrifugation, it is time saving
And it is easy to operate, realize in a short time it is convenient, fast, efficiently extract PBMC, simplify operating procedure.
By contrast, there is the difference of essence with above-mentioned patent publication us in present patent application.
The content of the invention
The goal of the invention of the present invention is overcome the deficiencies in the prior art, there is provided one kind is simple in construction, design science is reliable,
After quantitative cell separating liquid is filled in cell Separating tube bottom, not only convenient transportation, and it can directly be operated with, save
Substantial amounts of human and material resources, easy to use, the cell with sieve plate separation and Extraction PBMC to be brought great convenience to user
Separating pipe, and the separating pipe uses simple operation, more can fast and efficiently extract PBMC.
The present invention is realized using following technical scheme：
A kind of cell Separating tube with sieve plate separation and Extraction PBMC, the separating pipe include lid, body, isolation sieve plate and
Separating liquid, lid coaxial threaded engagement sealing shroud are loaded on the top of body, and the bottom and inboard wall of tube body in the body are same
Sealing installation isolation sieve plate, isolate uniform interval on sieve plate and be formed with several sieve apertures, the body inner bottom part below the isolation sieve plate
Encapsulate cell separating liquid.
Moreover, the tapered shape in the bottom of the body, the isolation sieve plate is cylindrical, and the bottom in the body is coaxial
Be spaced it is upper and lower inwardly make the raised and lower annular projection of upper annular, be arranged in parallel between the raised and lower annular projection of the upper annular,
The marginal portion of installation isolation sieve plate is fitted together between the raised and lower annular projection of the upper annular.
Moreover, the width of the lower annular projection, height are respectively greater than the raised width of upper annular, height, the isolation
The nominal diameter of sieve plate is consistent with the nominal bore diameter of cell Separating tube, isolates a diameter of negative common difference of sieve plate, cell Separating tube
Internal diameter be plus tolerance, the two inside and outside footpath cooperates.
Moreover, the volume of the separating pipe is 50ml scale loading amounts, isolation sieve plate is set at the 15ml of the separation inside pipe wall.
Moreover, the bottom of the body is in arc-shaped, the isolation sieve plate is in the arc-shaped to raise up.
Moreover, the volume of the separating pipe is 10ml scale loading amounts, isolation sieve plate is set at the 3ml of the separation inside pipe wall.
Moreover, the nominal diameter of the isolation sieve plate is consistent with the nominal bore diameter of cell Separating tube, isolate the straight of sieve plate
Footpath is plus tolerance, is mutually interference fit with separating bore.
The advantages and positive effects of the present invention are：
1st, due to body is divided into upper and lower two part provided with isolation sieve plate, isolation sieve plate, separating liquid is encapsulated in this separating pipe
The body inner bottom part isolated below sieve plate, after the filling separating liquid of separating pipe, by centrifugally operated, due to being covered with sieve aperture on sieve plate,
Separating liquid can be all collected into the separating pipe below sieve plate by sieve aperture, and the design of the isolation sieve plate can be quickly by blood sample
Originally it is added on the liquid level of separating liquid and isolates above sieve plate, and can prevents blood preparation from being mixed with the separating liquid below sieve plate,
Moreover, isolation sieve plate also efficiently avoid be infused in isolation sieve plate below separating liquid separating pipe be inverted, transport jolt
Under vibrations or collision situation, the top of the isolation sieve plate of " reflux " to separating pipe, ensure that cell separating liquid is constantly in isolation sieve
In the separating pipe of the lower section of plate, so that cell extraction operation is more reliable, conveniently, cell Separating tube transports also more square
Just.
2nd, this separating pipe centrifuge blood preparation when, in the centrifugal process of separate blood cells, can rapidly by
Red blood cell etc. is deposited in the lower section of cell Separating tube through the hole of sieve plate in blood sample, blood platelet in sample blood, red thin
Born of the same parents may pass through sieve aperture layering and precipitating in centrifugation in the exhausted big portion in its bottom isolated below sieve plate, and required for centrifuging
Dividing PBMC cells, then separated liquid and isolation sieve plate are retained in the separating pipe above isolation sieve plate, will directly can be separated after centrifugation
The PBMC that goes out is quick, easily pours into new container, takes out light, easy to operate, quick, also saves the time, improves point
Cellifugal quality, operating procedure is simplified, efficiently extracts PBMC.
3rd, the tapered shape in the bottom of the body of this separating pipe so that reaction solution mixes more abundant, uniformly, and upper annular is convex
Rise and lower annular projection setting, enable to isolate sieve plate can be accurately positioned and can prevent isolate sieve plate enter body under
Portion, so as to lose its effect, the stability of the structure of the separating pipe is ensure that, while also extend the use longevity of the separating pipe
Life.
4th, the structure of this separating pipe is more accurate, and measurement is more accurate.
Brief description of the drawings
Fig. 1 is a kind of structure connection diagram of the present invention；
Fig. 2 is the top perspective view for isolating sieve plate in Fig. 1；
Fig. 3 is the A portions structure enlargement diagram in Fig. 1；
Fig. 4 is another structure connection diagram of the present invention；
Fig. 5 is the top perspective view for isolating sieve plate in Fig. 4.
Embodiment
For present disclosure, feature and effect can be further appreciated that, following examples are hereby enumerated, and coordinate accompanying drawing detailed
It is described as follows.It should be noted that the present embodiment is descriptive, it is not limited, it is impossible to thus limit the guarantor of the present invention
Protect scope.
A kind of cell Separating tube with sieve plate separation and Extraction PBMC, as shown in Figure 1 and Figure 4, the separating pipe include lid
1st, body 2, isolation sieve plate 4 and separating liquid 6, the lid coaxial threaded engagement sealing shroud are loaded on the top of body, the body
Sieve plate isolate in the installation of interior bottom and inboard wall of tube body co-axial seal, isolates on sieve plate being uniformly spaced that to be formed with several sieve apertures 8 (such as micro-
Small sieve aperture), the body inner bottom part encapsulation cell separating liquid below the isolation sieve plate, after the filling quantitative cell separating liquid of separating pipe,
By centrifugally operated, cell separating liquid can all be collected into the separating pipe intracavitary below sieve plate by sieve aperture, now pre-install cell
The extraction PBMC cell Separating tubes of separating liquid can be used as whole medical treatment equipment and be supplied to demander.
This separating pipe due to body is divided into upper and lower two part provided with isolation sieve plate, isolation sieve plate, separating liquid be encapsulated in every
From the body inner bottom part below sieve plate, after the filling separating liquid of separating pipe, by centrifugally operated, due to being covered with sieve aperture on sieve plate, point
Chaotropic can be all collected into the separating pipe below sieve plate by sieve aperture, and the design of the isolation sieve plate can be quickly by blood sample
It is added on the liquid level of separating liquid and isolates above sieve plate, and can prevents blood preparation from being mixed with the separating liquid below sieve plate, and
And isolation sieve plate also efficiently avoid the shake of jolting that the separating liquid being infused in below isolation sieve plate is inverted, transported in separating pipe
Under dynamic or collision situation, the top of the isolation sieve plate of " reflux " to separating pipe, ensure that cell separating liquid is constantly in isolation sieve plate
Lower section separating pipe in so that cell extraction operation is more reliable, conveniently, cell Separating tube transports also more convenient.
, can be rapidly by blood in the centrifugal process of separate blood cells when this separating pipe centrifuges blood preparation
Red blood cell etc. is deposited in the lower section of cell Separating tube through the hole of sieve plate in liquid sample, blood platelet, red blood cell in sample blood
Sieve aperture layering and precipitating is may pass through in centrifugation in the overwhelming majority in its bottom isolated below sieve plate, and required for centrifuging
Then separated liquid and isolation sieve plate are retained in the separating pipe above isolation sieve plate PBMC cells, will directly can be isolated after centrifugation
PBMC it is quick, easily pour into new container, take out light, easy to operate, quick, also save the time, improve separation
The quality of cell, operating procedure is simplified, efficiently extracts PBMC.
In the present embodiment, as shown in Figure 1, Figure 2 and Figure 3, the 7 tapered shape of bottom of the body so that reaction solution mixes
Close more abundant, uniformly, the isolation sieve plate is cylindrical, and the bottom coaxial spaced in the body is upper and lower inwardly to make
Upper annular projection 3 and lower annular projection 5, be arranged in parallel between the raised and lower annular projection of the upper annular, the upper annular it is raised and
The marginal portion of installation isolation sieve plate is fitted together between lower annular projection.The setting of the raised and lower annular projection of the upper annular, can
Isolation sieve plate is accurately positioned and can prevent from isolating the bottom that sieve plate enters body, so as to lose its effect, ensure that
The stability of the structure of the separating pipe, while also extend the service life of the separating pipe.
The width of the lower annular projection, height are slightly larger than the raised width of upper annular, height, the isolation sieve plate respectively
Nominal diameter it is consistent with the nominal bore diameter of cell Separating tube, isolate a diameter of negative common difference of sieve plate, cell Separating tube it is interior
Footpath is plus tolerance, and the two inside and outside footpath cooperates so that the separation tubular construction is more accurate, and measurement is more accurate；The separation
The volume of pipe can be 50ml scale loading amounts, and isolation sieve plate is set at the 15ml of the separation inside pipe wall.
In the present embodiment, as shown in Figure 4 and Figure 5, the bottom of the body is in arc-shaped, and the isolation sieve plate is in upward
Raised arc-shaped, the bottom of body are worked in coordination with isolating sieve plate so that reaction solution mixes more abundant, uniformly.
The volume of the separating pipe can be 10ml scale loading amounts, and isolation sieve plate is set at the 3ml of the separation inside pipe wall；Institute
It is consistent with the nominal bore diameter of cell Separating tube to state the nominal diameter of isolation sieve plate, isolates a diameter of plus tolerance of sieve plate, with dividing
Mutually it is interference fitted each other from bore so that the separation tubular construction is more accurate, and measurement is more accurate.
The application method of this cell Separating tube with sieve plate separation and Extraction PBMC is as follows：
Due to the pre- canned Quantitative Separation liquid of the cell Separating tube with sieve plate separation and Extraction PBMC, after backing out lid,
By blood preparation along isolation sieve plate above inboard wall of tube body be directly injected into separating pipe, cover lid, the centrifuge tube be put into from
Separated in scheming with 1200*g centrifugal force；After separation, the different presentation liquid layereds of cell Separating tube internal cause density are red thin
Born of the same parents in the bottom, be successively up granulocyte, separating liquid, mononuclearcell (tunica albuginea layer), the wherein blood plasma of dilution, tunica albuginea layer just
It is the monocyte for needing to extract, directly can be easily poured into the PBMC isolated in new centrifuge tube after centrifugation, use is dilute
Release liquid and clean again secondary, the PBMC of required extraction is just deposited in the bottommost of test tube, taking-up.
Claims (3)
- A kind of 1. cell Separating tube with sieve plate separation and Extraction PBMC, it is characterised in that：The separating pipe include lid, body, Isolate sieve plate and separating liquid, the lid coaxial threaded engagement sealing shroud is loaded on the top of body, the bottom in the body with Inboard wall of tube body co-axial seal installation isolation sieve plate, isolate uniform interval on sieve plate and be formed with several sieve apertures, below the isolation sieve plate Body inner bottom part encapsulates cell separating liquid；The tapered shape in bottom of the body, the isolation sieve plate is cylindrical, and the bottom coaxial spaced in the body is upper and lower The raised and lower annular projection of upper annular inwardly is made, is be arranged in parallel between the raised and lower annular projection of the upper annular, the upper annular The marginal portion of installation isolation sieve plate is fitted together between raised and lower annular projection；The width of the lower annular projection, height are respectively greater than the raised width of upper annular, height, the nominal of the isolation sieve plate Diameter is consistent with the nominal bore diameter of cell Separating tube, isolates a diameter of negative common difference of sieve plate, the internal diameter of cell Separating tube is just Tolerance, the two inside and outside footpath cooperate；The bottom of the body is in arc-shaped, and the isolation sieve plate is in the arc-shaped to raise up；The nominal diameter of the isolation sieve plate is consistent with the nominal bore diameter of cell Separating tube, isolates a diameter of positive public of sieve plate Difference, it is mutually interference fit with separating bore.
- 2. the cell Separating tube according to claim 1 with sieve plate separation and Extraction PBMC, it is characterised in that：The separating pipe Volume be 50ml scale loading amounts, isolation sieve plate is set at the 15ml of the separation inside pipe wall.
- 3. the cell Separating tube according to claim 1 with sieve plate separation and Extraction PBMC, it is characterised in that：The separating pipe Volume be 10ml scale loading amounts, isolation sieve plate is set at the 3ml of the separation inside pipe wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510664306.1A CN105132278B (en) | 2015-10-15 | 2015-10-15 | Cell Separating tube with sieve plate separation and Extraction PBMC |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510664306.1A CN105132278B (en) | 2015-10-15 | 2015-10-15 | Cell Separating tube with sieve plate separation and Extraction PBMC |
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| Publication Number | Publication Date |
|---|---|
| CN105132278A CN105132278A (en) | 2015-12-09 |
| CN105132278B true CN105132278B (en) | 2017-12-05 |
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| CN201510664306.1A Active CN105132278B (en) | 2015-10-15 | 2015-10-15 | Cell Separating tube with sieve plate separation and Extraction PBMC |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587884B (en) * | 2018-07-16 | 2023-07-25 | 苏州呼呼健康科技有限公司 | Centrifuge tube |
| CN110591901A (en) * | 2019-10-27 | 2019-12-20 | 李文强 | Cell separation tube |
| CN117282129B (en) * | 2023-11-24 | 2024-02-02 | 南昌大学第一附属医院 | Serum bilirubin removing device and method |
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| CN2892868Y (en) * | 2005-12-06 | 2007-04-25 | 刘瑞东 | Cell detaching tube |
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| JP2009038989A (en) * | 2007-08-06 | 2009-02-26 | Olympus Corp | Cell separation vessel |
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| CN204022830U (en) * | 2014-08-19 | 2014-12-17 | 任峰 | A kind of cell Separating tube for separating of extracting PBMC |
| CN104651224A (en) * | 2015-02-10 | 2015-05-27 | 张丽红 | Double-tube vacuum film sperm separation method and separation device |
| CN205115470U (en) * | 2015-10-15 | 2016-03-30 | 任峰 | Belt screen board separation and extraction PBMC's cell separator tube |
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| US7323144B2 (en) * | 2002-03-18 | 2008-01-29 | Leisure, Inc. | Apparatus for separating biological sample and separating method of the same |
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| US4857463A (en) * | 1986-04-19 | 1989-08-15 | Medical Research International Ltd. | Method of preparing intracellular parasites for storage |
| CN2892868Y (en) * | 2005-12-06 | 2007-04-25 | 刘瑞东 | Cell detaching tube |
| CN200940146Y (en) * | 2006-08-18 | 2007-08-29 | 王茜 | Sieve like large intestine cells collector |
| JP2009038989A (en) * | 2007-08-06 | 2009-02-26 | Olympus Corp | Cell separation vessel |
| CN201901672U (en) * | 2010-03-31 | 2011-07-20 | 周遵善 | Separation device for adipose-derived stem cells |
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| CN204022830U (en) * | 2014-08-19 | 2014-12-17 | 任峰 | A kind of cell Separating tube for separating of extracting PBMC |
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| Publication number | Publication date |
|---|---|
| CN105132278A (en) | 2015-12-09 |
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