CN108587884B - Centrifuge tube - Google Patents

Abstract

The invention provides a centrifuge tube, and belongs to the technical field of cell separation. Comprises an upper pipe body, a lower pipe body, a filtering membrane and a pipe cover. The upper pipe body is of a structure with openings at the top and the bottom. The upper pipe body is of a top end opening structure, the bottom end of the upper pipe body is detachably inserted into the lower pipe body, and the outer side wall of the upper pipe body is in sealing fit with the inner side wall of the lower pipe body. The filtering membrane is plugged at the bottom end of the upper tube body, and can allow the separation liquid to pass through to prevent the mononuclear cells from passing through. The pipe cover is covered on the top end of the upper pipe body. The filtering membrane can allow the separating liquid to pass through and has a blocking effect on the mononuclear cells, the separating liquid and the red blood cells are positioned below the filtering membrane, and the white membrane layer where the plasma and the mononuclear cells are positioned is positioned above the filtering membrane, so that the white membrane layer is effectively isolated from the separating liquid and the red blood cells, and the purity of the separated mononuclear cells is ensured.

Description

Centrifuge tube

Technical Field

The invention relates to the technical field of cell separation, in particular to a centrifuge tube.

Background

Currently, peripheral blood mononuclear cells (PBMC cells) include lymphocytes and monocytes. In vitro lymphocyte detection, peripheral blood mononuclear cells are first separated, and the main separation method is density gradient centrifugation, which is used for separating blood components due to the difference in specific gravity. The density of the red blood cells is larger than that of the separating liquid, and the red blood cells are deposited at the bottom of the tube; platelets are suspended in the plasma due to the small density, and only mononuclear cells corresponding to the separation liquid density are densely arranged in the interface between the plasma layer and the separation liquid and are in a white membrane shape, namely, the white membrane layer, and the mononuclear cells can be obtained by sucking the white membrane layer.

In the actual operation process, the suction pipe is required to be inserted into the white membrane layer where the mononuclear cells are located so as to suck the mononuclear cells in the layer, and the separation liquid and the red blood cells positioned at the lower layer of the white membrane layer are easy to suck in the process of sucking the mononuclear cells, so that the purity of the mononuclear cells is difficult to ensure.

Disclosure of Invention

The invention aims to provide a centrifuge tube, which aims to solve the problem that the purity of separated mononuclear cells is difficult to ensure in the conventional centrifuge tube.

The invention is realized in the following way:

based on the above object, the present invention provides a centrifuge tube comprising:

the upper pipe body is of a structure with openings at the top and the bottom;

the upper pipe body is of a top end opening structure, the bottom end of the upper pipe body is detachably inserted into the lower pipe body, and the outer side wall of the upper pipe body is in sealing fit with the inner side wall of the lower pipe body;

the filtering membrane is plugged in the opening at the bottom end of the upper tube body and can allow the separation liquid to pass through to prevent the mononuclear cells from passing through;

and the pipe cover is covered on the top end of the upper pipe body.

In one embodiment of the invention, the upper pipe body and the lower pipe body are provided with lifting structures;

when the upper pipe body rotates relative to the lower pipe body, the upper pipe body can axially move relative to the lower pipe body.

In one embodiment of the invention, the lifting structure comprises a protruding part arranged on the outer side wall of the upper pipe body and a chute arranged on the inner side wall of the lower pipe body, wherein the protruding part is clamped in the chute;

the sliding chute comprises a first groove section, a second groove section, a third groove section and a fourth groove section which are sequentially communicated, wherein the first groove section and the third groove section are arranged along the circumferential direction of the lower pipe body, the second groove section is a spiral groove, the fourth groove section is arranged along the axial direction of the lower pipe body, and the fourth groove section penetrates through the top end of the lower pipe body;

when the protruding part moves in the spiral groove, the upper pipe body can axially move relative to the lower pipe body.

In one embodiment of the invention, the centrifuge tube further comprises an inner tube and a closure;

the inner pipe body is arranged in the upper pipe body, the inner pipe body can axially move relative to the upper pipe body;

the sealing piece is used for opening or closing the liquid outlet.

In one embodiment of the present invention, the sealing member is rotatably connected to the inner tube, and the sealing member can rotate around the axis of the inner tube relative to the inner tube to open or close the liquid outlet.

In one embodiment of the invention, the inner side wall of the inner pipe body is provided with a ring protrusion, and the sealing piece is positioned between the ring protrusion and the bottom wall of the inner pipe body.

In one embodiment of the invention, the sealing piece is provided with a plug connector, the plug connector is movably arranged on the sealing piece, and the plug connector can vertically move relative to the sealing piece;

the bottom wall of the inner pipe body is provided with a jack, and when the sealing piece closes the liquid outlet, the plug connector can be inserted into the jack so as to limit the sealing piece to rotate relative to the inner pipe body.

In one embodiment of the invention, the centrifuge tube further comprises a rotating shaft, the rotating shaft and the inner tube body are coaxially arranged, the rotating shaft is rotatably arranged on the inner tube body, one end of the rotating shaft is fixedly connected with the sealing piece, and the other end of the rotating shaft penetrates through the top wall of the inner tube body and is positioned in the upper tube body.

In one embodiment of the present invention, a limiting structure is disposed between the inner tube and the upper tube to limit rotation of the inner tube relative to the upper tube.

In one embodiment of the present invention, the limiting structure includes a protrusion disposed on an outer sidewall of the inner pipe body and a clamping groove disposed on an inner sidewall of the upper pipe body, the clamping groove is disposed along an axial direction of the upper pipe body, the clamping groove penetrates through a top end of the upper pipe body, and the protrusion is clamped in the clamping groove;

when the inner pipe body axially moves relative to the upper pipe body, the protrusion can move in the clamping groove.

The beneficial effects of the invention are as follows:

the invention provides a centrifuge tube, which comprises an upper tube body, a lower tube body, a filtering membrane and a tube cover, wherein the upper tube body is detachably inserted into the lower tube body, the filtering membrane is arranged at the bottom end of the upper tube body, and the tube cover is arranged at the top end of the upper tube body. Blood and separating liquid are injected into the centrifuge tube, and after centrifugal treatment, the filtering membrane can allow the separating liquid to pass through, meanwhile, the separating liquid and the red blood cells are located below the filtering membrane, and the white membrane layer where the blood plasma and the single nuclear cells are located is located above the filtering membrane, so that the white membrane layer is effectively isolated from the separating liquid and the red blood cells, and the purity of the separated single nuclear cells is ensured. Because the upper tube body is detachably connected with the lower tube body, after the upper tube body is taken down, only the tunica albuginea layer and the plasma positioned on the upper side of the tunica albuginea layer are arranged in the upper tube body, and the tunica albuginea layer positioned on the lower layer is sucked through the suction tube, so that the operation is simple and convenient.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a centrifuge tube according to example 1 of the present invention;

FIG. 2 is an expanded view of the lower tubular body shown in FIG. 1;

FIG. 3 is a schematic diagram of a centrifuge tube according to example 2 of the present invention;

fig. 4 is a schematic view showing the connection of the inner tube, the sealing member and the rotating shaft shown in fig. 3.

Icon: 100-centrifuging tube; 10-an upper pipe body; 11-a projection; 12-clamping grooves; 20-lower pipe body; 21-a chute; 211-a first trough section; 212-a second trough section; 213-a third groove section; 214-a fourth trough section; 30-filtering the membrane; 40-tube cap; 50-an inner tube body; 51-accommodating space; 52-a liquid inlet; 53-a liquid outlet; 54-ring bulge; 55-bulge; 56-jack; 60-sealing piece; 70-rotating shaft; 80-plug-in connector; 90-spring.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

In the description of the embodiments of the present invention, it should be noted that, the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship conventionally put in use of the product of the present invention as understood by those skilled in the art, merely for convenience of describing the present invention and simplifying the description, and is not indicative or implying that the apparatus or element to be referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.

In the description of the embodiments of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; may be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

Example 1

As shown in fig. 1, this embodiment provides a centrifuge tube 100, which includes an upper tube body 10, a lower tube body 20, a filtering membrane 30 and a tube cover 40, wherein the bottom end of the upper tube body 10 is detachably inserted into the lower tube body 20, the filtering membrane 30 is disposed at the bottom end of the upper tube body 10, and the tube cover 40 covers the top end of the upper tube body 10.

Wherein, the upper tube body 10 is cylindrical, and has a hollow structure with two open ends at the top and the bottom. In this embodiment, the outer sidewall of the upper tube body 10 is provided with a protruding portion 11, the protruding portion 11 is cylindrical, the axial direction of the protruding portion 11 is consistent with the radial direction of the upper tube body 10, and the protruding portion 11 is close to the bottom end of the upper tube body 10.

The lower pipe body 20 is cylindrical, the lower pipe body 20 is of a top end opening structure, and the inner diameter of the lower pipe body 20 is matched with the outer diameter of the upper pipe body 10.

The inner side wall of the lower pipe body 20 is provided with a chute 21, and the width of the chute 21 is consistent with the diameter of the protruding part 11 on the outer side wall of the upper pipe body 10. The chute 21 includes a first chute section 211, a second chute section 212, a third chute section 213, and a fourth chute section 214, which are sequentially communicated. Wherein, the first groove section 211 and the third groove section 213 are arranged along the circumferential direction of the lower pipe body 20, and the position of the third groove section 213 is higher than the position of the first groove section 211; the second groove section 212 is a spiral groove, and the first groove section 211 is transited to the third groove section 213 through the second groove section 212; the fourth groove section 214 is disposed along the axial direction of the lower tube body 20, and the fourth groove section 214 penetrates the top end of the lower tube body 20. As shown in fig. 2, after the inner side wall of the lower pipe body 20 is unfolded to be a plane, the first groove section 211 and the third groove section 213 are horizontally arranged, the second groove section 212 is obliquely arranged, and the fourth groove section 214 is vertically arranged.

The filter membrane 30 is provided with filter pores, the diameter of which is slightly smaller than the size of the mononuclear cells. The filter membrane is capable of allowing the passage of the separation liquid, but of course it is also capable of allowing the passage of erythrocytes, but the filter membrane 30 is not capable of allowing the passage of mononuclear cells, i.e. the filter membrane 30 has the capacity to prevent the passage of mononuclear cells.

As shown in fig. 1, the bottom end of the upper pipe body 10 is inserted into the lower pipe body 20, the outer side wall of the upper pipe body 10 is in sealing fit with the inner side wall of the lower pipe body 20, and the protruding part 11 on the outer side wall of the upper pipe body 10 is clamped in the sliding groove 21 on the inner side wall of the lower pipe body 20; the filtering membrane 30 is blocked at the opening at the bottom end of the upper pipe body 10, and the filtering membrane 30 and the upper pipe body 10 are fixed in an adhesive mode; the tube cover 40 is covered at the top end opening of the upper tube body 10, and the tube cover 40 is in threaded connection with the upper tube body 10.

After the upper pipe body 10 is in butt joint with the lower pipe body 20, the protruding part 11 on the outer side wall of the upper pipe body 10 and the sliding groove 21 on the inner side wall of the lower pipe body 20 form a lifting structure between the upper pipe body 10 and the lower pipe body 20, namely, when the upper pipe body 10 rotates relative to the lower pipe body 20, the upper pipe body 10 can axially move relative to the lower pipe body 20. In a normal state, the protruding portion 11 is clamped in the first groove section 211 of the chute 21, at this time, a certain distance exists between the filtering membrane 30 and the lower tube 20, and the lower tube 20 has a certain space for accommodating red blood cells and separating liquid. When the upper pipe body 10 rotates relative to the lower pipe body 20 under the action of external force, the protruding part 11 slides into the second groove section 212 from the first groove section 211, and the protruding part 11 slides in the second groove section 212 and moves upwards relative to the lower pipe body 20 axially at the same time as the second groove section 212 is a spiral groove, so that the distance between the filtering membrane 30 and the bottom wall of the lower pipe body 20 is increased, and the accommodating space of the lower pipe body 20 is increased; continued rotation of the upper tubular body 10 will move the projection 11 from the second slot segment 212 to the third slot segment 213, and the upper tubular body 10 will not move axially as the projection 11 moves within the first and third slot segments 211, 213; the projection 11 eventually moves to the junction of the third slot segment 213 and the fourth slot segment 214, and in this state, lifting the upper pipe 10 upward can disengage the upper pipe 10 from the lower pipe 20.

When in use, the tube cover 40 is opened, lymphocyte separation liquid and blood are poured from the top end opening of the upper tube body 10, and the tube cover 40 is covered, at this time, the separation liquid is arranged in the upper tube body 10 and the lower tube body 20; the whole centrifuge tube 100 is put into a centrifuge for centrifugation, during the centrifugation, when the lower tube body 20 rotates along with the centrifuge, the upper tube body 10 is temporarily kept still under the action of inertia, so that the upper tube body 10 and the lower tube body 20 relatively rotate, the convex part 11 moves from the first groove section 211 to the third groove section 213, the upper tube body 10 moves upwards axially, and the space for containing separating liquid and red blood cells in the lower tube body 20 is increased; in the process of axially moving the upper pipe body 10 upwards, the separation liquid in the upper pipe body 10 cannot enter the lower pipe body 20 through the filtering membrane 30 under the action of centrifugal force; after the centrifugation is completed, the separation liquid in the upper tube body 10 will be in a stationary state, and the separation liquid and red blood cells in the upper tube body 10 will enter the lower tube body 20 through the filtering membrane 30 (the red blood cells are located at the lower side of the separation liquid), while the white membrane layer where the plasma and mononuclear cells are located will be blocked by the filtering membrane 30, and the plasma and white membrane layer will be located in the upper tube body 10 (the plasma is located at the upper side of the white membrane layer) because the upper tube body 10 moves upward to vacate the redundant viewing space. Thereby effectively isolating the white membrane layer from the separating liquid and the red blood cells and ensuring the purity of the separated mononuclear cells; finally, after the upper tube body 10 is taken down, namely, the protruding part 11 slides out from the fourth groove section 214, only the tunica albuginea layer and the plasma positioned on the upper side of the tunica albuginea layer are arranged in the upper tube body 10, and the tunica albuginea layer positioned on the lower layer is sucked through the suction tube, so that the operation is simple and convenient.

Example 2

As shown in fig. 3, this embodiment provides a centrifuge tube 100 that differs from the previous embodiments in that it further includes an inner tube 50, a sealing member 60, and a rotating shaft 70.

Wherein the inner tube 50 is cylindrical, and the outer diameter of the inner tube 50 is matched with the inner diameter of the upper tube 10. The inner tube 50 is internally provided with a cylindrical accommodating space 51, the inner tube 50 is provided with a top wall and a bottom wall, the top wall of the inner tube 50 is provided with a liquid inlet 52, the bottom wall of the inner tube 50 is provided with a liquid outlet 53, and the liquid inlet 52 and the liquid outlet 53 are communicated with the accommodating holes. In this embodiment, the number of liquid inlets 52 is plural, the number of liquid outlets 53 is one, and the liquid outlets 53 are not disposed at the center of the bottom wall of the inner tube 50.

In addition, in the present embodiment, the inner sidewall of the inner tube 50 is provided with a ring protrusion 54, the ring protrusion 54 has a ring structure, the ring protrusion 54 is coaxially disposed with the inner tube 50, and a gap exists between the ring protrusion 54 and the bottom wall of the inner tube. The outer side wall of the inner tube is provided with a bulge 55, the bulge 55 is cylindrical, and the axial direction of the bulge 55 is consistent with the radial direction of the inner tube body 50.

In this embodiment, a clamping groove 12 is provided on the inner wall of the upper tube body 10, the clamping groove 12 is arranged along the axial direction of the upper tube body 10, and the clamping groove 12 penetrates through the top end of the upper tube body 10.

The inner tube 50 is arranged in the upper tube 10, the outer side wall of the inner tube 50 is in sealing fit with the inner side wall of the upper tube 10, and the protrusions 55 on the outer side wall of the inner tube 50 are clamped in the clamping grooves 12 on the inner side wall of the upper tube 10. After the inner tube 50 is disposed in the upper tube 10, the inner tube 50 can move axially relative to the upper tube 10. When the inner tube 50 moves axially relative to the upper tube 10, the projection 55 will move within the catch 12. The protrusion 55 on the outer side wall of the inner tube 50 and the clamping groove 12 on the inner side wall of the upper tube 10 form a limiting structure between the inner tube 50 and the upper tube 10, so that the inner tube 50 is limited to rotate relative to the upper tube 10.

The closure 60 functions to open or close the liquid outlet 53 on the inner tubular body 50. In this embodiment, the sealing member 60 is semicircular, and the outer diameter of the sealing member 60 matches the inner diameter of the inner tube 50. The closure 60 is rotatably connected to the inner tube 50, the closure 60 opening or tube outlet 53 in a rotational manner.

In this embodiment, the sealing member 60 is rotatably connected with the inner tube 50 through the rotating shaft 70, the sealing member 60 is disposed between the annular protrusion 54 and the bottom wall of the inner tube 50, the upper surface of the sealing member 60 contacts with the annular protrusion 54 and forms a sealing fit, and the lower surface of the sealing member 60 contacts with the bottom wall of the inner tube 50 and forms a sealing fit; the rotating shaft 70 is rotatably arranged on the inner pipe body 50, one end of the rotating shaft 70 is fixedly connected with the sealing piece 60, and the other end of the rotating shaft 70 penetrates through the top wall of the inner pipe body 50 and is positioned in the upper pipe body 10; the closure 60 and the shaft 70 are both coaxially disposed with the inner tubular body 50. When the shaft 70 rotates, the shaft 70 will rotate the closure 60 about the axis of the inner tube 50. When the sealing piece 60 is covered above the liquid outlet 53, the sealing piece 60 closes the liquid outlet 53; when the sealing member 60 is offset from the liquid outlet 53, the liquid outlet 53 is opened.

As shown in fig. 4, in this embodiment, the lower surface of the sealing member 60 is provided with a receiving hole, in which a plug member 80 is disposed, the plug member 80 is cylindrical, and the plug member 80 can move vertically in the receiving hole. In addition, a spring 90 is arranged in the accommodating hole, and the spring 90 acts on the plug connector 80, so that the plug connector 80 has a vertical downward movement trend.

The bottom wall of the inner pipe body 50 is provided with a jack 56, and the jack 56 is a through hole. When the sealing member 60 rotates to a position for closing the liquid outlet 53, the plug member 80 is aligned with the jack 56, and the plug member 80 is inserted into the jack 56, so that the sealing member 60 is limited to rotate relative to the inner tube 50, and the liquid outlet 53 is in a closed state consistently.

In use, the tube cover 40 is opened, lymphocyte separation liquid and blood are poured from the top opening of the upper tube body 10, and the tube cover 40 is covered; placing the whole centrifuge tube 100 into a centrifuge for centrifugation, wherein after centrifugation, the separating liquid and the red blood cells are positioned in the lower tube body 20, and the white membrane layer and the plasma are positioned in the inner tube body 50; after the upper tube body 10 and the lower tube body 20 are separated, the white film layer and the blood plasma are always positioned in the inner tube body 50, and the white film layer is positioned at the lower side of the blood plasma; after the tube cover 40 is removed, an operator can hold the rotating shaft 70 to lift the inner tube body 50 upwards, so that the inner tube body 50 moves vertically upwards relative to the upper tube body 10, when the bottom wall of the inner tube body 50 moves to the boundary between the white film layer and the blood plasma, the rotating shaft 70 is rotated, the sealing piece 60 is rotated to a position for closing the liquid outlet 53, the plug connector 80 is inserted into the through hole under the action of the spring 90, at this time, the white film layer is positioned in the upper tube body 10, and the blood plasma is positioned in the inner tube body 50; after the inner tube 50 is separated from the upper tube 10, a white film layer with higher purity in the upper tube 10 is finally obtained.

The embodiment provides the centrifuge tube 100, which can separate the plasma from the tunica albuginea layer rapidly and conveniently, and ensures the purity of the mononuclear cells in the tunica albuginea layer.

The rest of the structure of this embodiment is the same as that of embodiment 1, and will not be described here again.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (7)

1. A centrifuge tube, comprising:

the upper pipe body is of a structure with openings at the top and the bottom;

the upper pipe body is of a top end opening structure, the bottom end of the upper pipe body is detachably inserted into the lower pipe body, and the outer side wall of the upper pipe body is in sealing fit with the inner side wall of the lower pipe body;

the filtering membrane is plugged in the opening at the bottom end of the upper tube body and can allow the separation liquid to pass through to prevent the mononuclear cells from passing through;

the pipe cover is covered on the top end of the upper pipe body;

the upper pipe body and the lower pipe body are provided with lifting structures; when the upper pipe body rotates relative to the lower pipe body, the upper pipe body can axially move relative to the lower pipe body; the lifting structure comprises a protruding part arranged on the outer side wall of the upper pipe body and a sliding groove arranged on the inner side wall of the lower pipe body, and the protruding part is clamped in the sliding groove; the sliding chute comprises a first groove section, a second groove section, a third groove section and a fourth groove section which are sequentially communicated, wherein the first groove section and the third groove section are arranged along the circumferential direction of the lower pipe body, the position of the third groove section is higher than that of the first groove section, the second groove section is a spiral groove, the fourth groove section is arranged along the axial direction of the lower pipe body, and the fourth groove section penetrates through the top end of the lower pipe body; when the protruding part moves in the spiral groove, the upper pipe body can axially move relative to the lower pipe body;

the centrifuge tube also comprises an inner tube body and a sealing piece; the inner pipe body is arranged in the upper pipe body, the inner pipe body can axially move relative to the upper pipe body; the sealing piece is used for opening or closing the liquid outlet.

2. The centrifuge tube of claim 1, wherein said closure is rotatably coupled to said inner tube, said closure being rotatable relative to said inner tube about an axis of said inner tube to open or close said liquid outlet.

3. The centrifuge tube of claim 2, wherein the inner sidewall of the inner tube body is provided with an annular protrusion, and the sealing member is positioned between the annular protrusion and the bottom wall of the inner tube body.

4. A centrifuge tube according to claim 3, wherein the closure is provided with a plug connector, the plug connector being movably arranged on the closure, the plug connector being vertically movable relative to the closure;

the bottom wall of the inner pipe body is provided with a jack, and when the sealing piece closes the liquid outlet, the plug connector can be inserted into the jack so as to limit the sealing piece to rotate relative to the inner pipe body.

5. The centrifuge tube of claim 2, further comprising a rotating shaft coaxially disposed with the inner tube, the rotating shaft rotatably disposed in the inner tube, one end of the rotating shaft fixedly connected to the sealing member, and the other end of the rotating shaft passing through the top wall of the inner tube and being disposed in the upper tube.

6. The centrifuge tube of any of claims 1-5, wherein a limiting structure is provided between the inner tube and the upper tube to limit rotation of the inner tube relative to the upper tube.

7. The centrifuge tube of claim 6, wherein the limiting structure comprises a protrusion arranged on the outer side wall of the inner tube body and a clamping groove arranged on the inner side wall of the upper tube body, wherein the clamping groove is arranged along the axial direction of the upper tube body, the clamping groove penetrates through the top end of the upper tube body, and the protrusion is clamped in the clamping groove;

when the inner pipe body axially moves relative to the upper pipe body, the protrusion can move in the clamping groove.