KR20220021236A - membrane centrifugal concentrator - Google Patents

Abstract

Disclosed is a membrane centrifugal concentrator which comprises an inlet part having a hollow shape with an open upper part so that a mixed sample flows into the inside, and a housing part formed at the lower part of the inlet part to communicate with the inside of the inlet part. The housing part includes a housing body having a hollow shape with a narrow cross section in the direction of centrifugal force acting during centrifugal separation, and a filter body formed to mount a filter paper on one side of the housing body. The filter body is formed with a plurality of filter holes passing through the inner and outer peripheral surfaces on one side so that a first sample passing through the filter paper can be discharged to the outside. According to the present invention, in the case of recovery of precipitate during centrifugation, the entire amount of the precipitate can be easily recovered. In the case of separation of a mixed sample using a filter paper, a separation procedure is simplified, and thus accuracy and reliability of test results can be increased.

Description

Membrane centrifugal concentrator

The present invention relates to a centrifugal concentration and filter used by separately mounting a membrane filter inside a centrifuge tube to separate, purify, and concentrate proteins, viruses, etc., such as antibodies. Accordingly, the present invention relates to a membrane centrifugal concentrator capable of stably and easily separating and concentrating a sample of a specific size from a mixed sample having different molecular weights.

The centrifugation method refers to a method of separating, purifying, and concentrating a sample or a sample having a different specific gravity using centrifugal force. The centrifugation method can be divided into a separation method and a filtration method. The separation method is a method of separation using the difference in specific gravity in a mixture in which suspended substances such as particulates and colloids are mixed. On the other hand, the filtration method is a method of separating a sample using the relative particle size according to whether the filter passes or not.

In particular, the centrifugal separation method using a separation method is a method in which a solution containing suspended matter is rotated at a high speed to precipitate the suspended matter and separate it from the solution.

In general, the centrifugation method uses a tube for centrifugation standardized by capacity. The mixed sample to be separated is injected into the centrifuge tube. Then, when the tube for centrifugation is rotated at high speed, the sediment is precipitated on the bottom surface of the tube for centrifugation due to the difference between centrifugal force and specific gravity, and the solution layer is suspended on the upper surface of the sediment. At this time, the solution layer in the separated sample can be separated from the precipitate by being discharged in a pouring form.

On the other hand, if you want to separate the solution in the centrifugation method, extraction is performed only by discharging the solution in the form of pouring from the separated sample. Extraction can be made by scooping using or washing with distilled water to recover and drying. For this reason, it is difficult and complicated to extract the precipitate, and it is difficult to extract the entire amount of the precipitate.

In particular, when the amount of the sample or sediment is small, it is more difficult to extract the sediment. In addition, if the entire amount of sediment is not extracted, the accuracy or reliability of the inspection result is deteriorated. For this reason, it is installed in a clean bench (clean bench) in order to maintain the sterile state of the centrifuge tube for extracting the entire amount of the sediment, an additional procedure that requires the use of a suction machine and a pipette (pipet) is required.

Therefore, the conventional tube for centrifugation has difficulties in extracting the sample separated through centrifugation, and thus related prior arts have been proposed to solve this problem.

The proposed prior art is a separable centrifuge tube (Korean Patent Application Laid-Open No. 10-2012-0123816), which is a tubular main tube with an open top and a bottom, and a cap coupled to open and close the upper part of the main tube. and a recovery tube detachably coupled to the lower part of the main tube and having a concave inner shape to accommodate the sediment in the sample.

In the case of the prior art, in the case of the recovery of a small amount of sediment, there is still a limit to the recovery of the entire amount, which causes a decrease in the accuracy and reliability of the inspection result. In addition, in the case of separation of a mixed sample using filter paper, there is a problem of limited use.

Korean Patent Laid-Open Patent No. 10-2012-0123816 : Separable centrifuge tube

The present invention was created to solve the problems of the prior art, and an object of the present invention is to provide a membrane centrifugal concentrator capable of easily extracting a sample separated by centrifugation and recovering the entire amount of the precipitate.

In order to achieve the above object, the membrane centrifugal concentrator according to the present invention includes an inlet having a hollow top with an open top so that a mixed sample is introduced into the inside, and a housing formed at a lower portion of the inlet to communicate with the inside of the inlet. Including, wherein the housing part is provided with a housing body having a hollow shape in which the cross section is narrowed along the direction of centrifugal force applied during centrifugation, and a filter body formed to mount a filter paper on one side of the housing body, the filtering body comprising: It is characterized in that a plurality of filtration holes are formed to penetrate the inner and outer peripheral surfaces on one side so that the first sample passing through the filter paper can be discharged to the outside.

The housing body further includes a collecting part formed to communicate with the inner space of the housing body at the lower part of the housing body and formed to collect the second sample remaining without passing through the filter paper, wherein the collecting part has an upper part open to communicate with the inner space of the housing part It is characterized in that it is provided with a collecting body having a shape that gradually narrows as it extends in the direction of the centrifugal force.

The filtration body is provided on the inner surface and characterized in that it includes a guide having one end connected to the filtration hole so that the first sample passing through the filter paper can be guided to the filtration hole.

In addition, the collecting unit further includes a sliding unit formed to move along the upper surface of the collecting body to open a collecting space in which the second sample is collected in the collecting unit, wherein the sliding unit is mounted on the housing body to the collecting body. A first sliding member having a coupling groove formed along the upper surface of the first sliding member and a second coupling protrusion provided with a coupling protrusion to be coupled with the coupling groove along the formation direction of the coupling recess on the collecting body so that the collecting body slides on the first sliding member The sliding member and the collecting space are airtightly formed so that the cross section of the L-shaped sliding sealing material and the housing body to fill the gap created after the coupling protrusion and the coupling groove are fastened is formed so as to be able to move up and down, and in contact with the rear of the collecting body when descending. A fixing member that blocks the collecting body from sliding in an opening direction, a fixing magnet formed on the upper end of the fixing member, and a contact portion in contact with the housing body when the fixing member rises are provided, and the fixing member is It characterized in that it is provided with a body magnet coupled to the fixed magnet and magnetic force to maintain the raised state.

In addition, the collecting unit includes a rotating unit formed so that the upper portion of the collecting body is opened by rotating the rotating unit is installed on the corresponding surface of the housing body and the collecting body, the rotating member is formed to rotate the collecting body and the centrifuge A rotating sealing material formed along a corresponding surface of the housing body and the collecting body so that the collecting body and the housing body are airtight during centrifugation by A first velcro formed on the lower surface of the housing body and a second velcro formed so that the collecting body is fixed to the outer surface of the collecting body by fastening with the first velcro.

In addition, it includes a pressurizing unit which is introduced through the upper part of the inlet to pressurize the mixed sample, wherein the pressurizing unit includes a pressurizing plate formed so as to be able to move up and down inside the inlet so that the mixed sample does not remain inside the inlet. A pressure sealing material formed on the inner side of the inlet and a contact surface of the pressure plate and the pressure plate are fixed inside the housing body to move in the direction of the centrifugal force, and a holding rod guiding the lifting and lowering of the pressure plate is provided, wherein the holding rod is the pressure plate It is characterized in that it is fastened to penetrate vertically.

In addition, the pressure plate includes a first pressure plate formed to correspond to the inner upper end of the housing body so that the mixed sample accommodated in the housing body does not flow back to the inlet during centrifugation, and the outer extension direction of the first pressure plate a second pressing plate provided with a bending member to be bent at a predetermined angle with respect to the first pressing plate along the inner surface of the housing body, and one end of the bending member is connected to the first pressing plate and the other end is connected to the second pressing plate It is characterized in that it comprises a bending connecting member formed on the lower surface of the bending member.

According to the present invention, in the case of recovery of sediment during centrifugation, the entire amount of sediment can be easily recovered, and it is effective to simplify the separation procedure of a mixed sample using filter paper to improve the accuracy and reliability of the test result.

1 is a state diagram showing a first embodiment of a membrane centrifugal concentrator according to the present invention,
Figure 2 is a partially cut and separated perspective view according to the embodiment of Figure 1,
Figure 3 is a perspective view of the filtration body according to the embodiment of Figure 1,
4 to 5 are partial cross-sectional views and longitudinal cross-sectional views of a second embodiment of a filter for a centrifuge tube including a sliding unit,
6 to 7 are partial cross-sectional views and longitudinal cross-sectional views of a third embodiment of a filter for a centrifuge tube including a rotation unit,
8 is a partial cross-sectional view of a fourth chamber example of a filter for a centrifuge tube including a pressurizing unit according to the present invention.

Hereinafter, a membrane centrifugal concentrator according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings. Since the present invention can have various changes and can have various forms, specific embodiments are illustrated in the drawings and described in detail in the text. However, this is not intended to limit the present invention to the specific disclosed form, it should be understood to include all modifications, equivalents and substitutes included in the spirit and scope of the present invention. In describing each figure, like reference numerals have been used for like elements. With respect to the accompanying drawings, the dimensions of the structures are enlarged than actual for clarity of the present invention.

Terms such as first, second, etc. may be used to describe various elements, but the elements should not be limited by the terms. The above terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, a first component may be referred to as a second component, and similarly, a second component may also be referred to as a first component.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In the present application, terms such as “comprise” or “have” are intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features It is to be understood that it does not preclude the possibility of the presence or addition of numbers, steps, operations, components, parts, or combinations thereof.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

1 to 3 show a first embodiment of the present invention.

Referring to the drawings, the centrifuge tube 1 includes a main tube 20 , a tube cap 30 and a membrane centrifugal concentrator 10 of the present invention.

A centrifugal force acts on the membrane centrifugal concentrator 10 in a direction in which the centrifuge tube 1 is mounted on the centrifuge.

The main tube 20 is provided in the form of a cylinder in which the upper end of the hollow tube is opened along the direction of the centrifugal force, and the lower end is blocked to accommodate the mixed sample.

The membrane centrifugal concentrator 10 is coupled to the inside of the main tube 20 .

In this case, the tube cap 30 is coupled to the upper end of the main tube 20 to seal the inside of the main tube 20 .

The mixed sample is a mixture of materials having different specific gravity. The mixed sample of the present invention includes the first sample (2), which is a suspended solution passing through the membrane centrifugal concentrator (10), and the membrane centrifugal concentrator (10). ), but the second sample 3, which is a sediment remaining inside, is taken as an example, but the present invention is not limited by the drawings, and the composition and separation of the mixed sample may be variously changed.

The membrane centrifugal concentrator 10 of the present invention includes an inlet part 100 , a housing part 200 , and a collecting part 300 .

The inlet 100 has a hollow shape with an open top so that the mixed sample is introduced into the inlet 100 . The inlet 100 may be formed in a polygonal shape, such as a square, hexagon, or octagon, without having a hollow cross-section limited to a circular shape as shown in the drawings.

In addition, when the membrane centrifugal concentrator 10 of the present invention and the main tube 20 are combined, the upper end of the inlet 100 is caught protruding in a radial direction from the outer peripheral surface so as to be caught on the upper end of the main tube 20 . A portion 110 is formed.

The housing part 200 is formed in a lower portion of the inlet part 100 to communicate with the inside of the inlet part 100 and includes a housing body 210 , a filtering body 220 , and a filter paper 230 .

The housing body 210 has a hollow shape in which the cross section is narrowed along the centrifugal force action direction.

The filter body 220 is formed so that the filter paper 230 can be mounted on one side of the housing body 210 . The filter body 220 is a structure that can be detached from one side of the housing body 210, and may have a polygonal shape such as a circular plate or a triangular plate, but preferably a square plate shape as shown in the drawings.

In addition, along the inner edge of the filtering body 220, one side of the filter fixing protrusion 223 is sharp is formed.

The filter fixing protrusion 223 allows the filter paper 230 to be in close contact with the filtering body 220 to be mounted on the housing body 210 .

The filter paper 230 is mounted inside the filtering body 220 to filter out a sample having a particle size greater than or equal to a predetermined size from the mixed sample inside the housing body 210 . Therefore, by changing the filter paper 230, it is possible to easily separate the size of the particles desired by the user in the mixed sample.

The filtering body 220 has a plurality of filtering holes 221 formed on one side so as to pass through the inner and outer circumferential surfaces, and includes a guide 222 .

The filter hole 221 is formed in a predetermined size so that the first sample 2 that has passed through the filter paper 230 is discharged to the outside, and the formation position, number, and formation pattern are not limited to this embodiment and are formed in various shapes. can be

The guide 222 is provided on the inner surface of the filtering body 220 so that the first sample 2 passing through the filter paper 230 can be guided to the filtering hole 221 at one end of the filtering hole 221 . ) to be connected. And, the guide 222 is not limited to a square groove formed on the inner surface of the filtering body 220 as shown in the drawings, but may be formed in the form of a polygonal groove such as a triangular groove or a semi-circular groove.

In addition, although the guide 222 is not shown in the drawing, a protrusion shape protruding from the inner surface of the filtering body 220 toward the inner space of the housing body 210 is also possible.

The collecting unit 300 is provided with the collecting body 310 having a collecting space 320 formed with a predetermined space so that the second sample 3 is collected.

The collecting unit 300 is formed to communicate with the inner space of the housing body 210 , and is formed so that the second sample 3 remaining without passing through the filter paper 230 is collected.

The collecting body 310 is formed in a shape in which the upper part of the collecting part 300 is opened to communicate with the inner space of the housing part 200 and becomes narrower as it extends in the action direction of the centrifugal force.

Since the inlet part 100, the housing part 200 and the collecting part 300 come in direct contact with the mixed sample, it does not chemically react with the mixed sample so as not to change the characteristics of the mixed sample and is made of a chemically very stable material. It is preferable to form For example, the inlet part 100 , the housing part 200 , and the collecting part 300 may be formed of a Teflon material having excellent chemical inert properties.

In the membrane centrifugal concentrator 10 of the present invention according to the first embodiment, the guide 222 is provided in the filtration body 220 so that the first sample 2 is guided to the filtration hole 221 . There is an advantage in that the first sample 2 is easily discharged.

Meanwhile, FIGS. 4 to 5 are partial cross-sectional views according to a second embodiment of the present invention.

Elements having the same function as in the drawings shown above are denoted by the same reference numerals.

The collecting unit 300 of this embodiment is slid with respect to the housing body 210 to the housing unit 200 through the sliding unit 400 so that the inner space of the collecting unit 300 can be exposed to the outside. are connected

The sliding unit 400 has a first sliding member 410 provided such that the collecting unit 300 moves along the upper surface of the collecting body 310 to open the collecting space 320 to the collecting unit 300 . ), a second sliding member 420 , a sliding sealing member 430 and a fixing member 440 .

The first sliding member 410 is formed at the lower portion of the housing body 210 , and a coupling groove 411 is provided along a corresponding surface of the collecting body 310 .

The second sliding member 420 is formed on the upper portion of the collecting body 310, and at the upper end of the collecting body 310 so that the collecting body 310 is slidably coupled to the first sliding member 410. A coupling protrusion 421 is provided so as to be engaged with the coupling groove 411 along the formation direction of the coupling groove 411 .

The coupling protrusion 421 has a rectangular cross-section cut in the direction of centrifugal force, but although not shown in the drawing, it may be formed in a polygonal shape such as a circle or a triangle.

The coupling groove 411 and the coupling protrusion 421 are not limited to those shown in the drawings, and include a conventional technique capable of sliding coupling.

The sliding sealing material 430 is formed so that the collecting space 320 is hermetically introduced into an external gap between the coupling protrusion 421 and the coupling groove 411 after they are fastened.

In more detail, the sliding sealing material 430 includes an inlet portion 431 that is introduced into a spaced gap between the upper outer circumferential surface of the collecting body 310 and the lower side surface of the first sliding member 410, and the inlet A blocking portion 441 extending along the lower surface of the first sliding member 410 from the lower end of the portion 431 is provided. At this time, the cross-section formed of the inlet portion 431 and the blocking portion 441 is an L-shaped letter.

In addition, as the sliding sealing material 430, various materials that can improve the airtightness of the collecting unit 300 and effectively prevent leakage of the sample may be used, but for example, an O-ring made of a Teflon material (Teflon) O-ring).

The fixing member 440 is formed so as to be able to ascend and descend on the housing body 210, and when descending, contact the rear of the collecting body 310 to block the collecting body 310 from sliding in the opening direction. It includes a fixed magnet 441 , a body magnet 442 , and a fixed body 443 .

The fixing magnet 441 is formed on the upper end of the fixing member 440 and is made of a material having a magnetic force.

The body magnet 442 is provided in a contact portion that comes into contact with the housing body 210 when the fixing member 440 is raised, and the fixing magnet 441 and magnetic force are provided to maintain the fixing member 440 in an elevated state. combine with

The stationary magnet 441 and the body magnet 442 can be magnetically coupled to each other in various conventional techniques.

The fixed body 443 is formed to be elongated along the action direction of the centrifugal force so as to be drawn into the inside of the housing body 210 . In addition, the fixing body 443 is formed so as not to interfere with the sliding sealing member 430 when the fixing member 440 is lifted.

A fixed inlet 444 is provided inside the housing body 210 so that the fixing member 440 rises and is introduced into the housing body 210 , and the fixed inlet 444 is located below the fixed inlet 444 . A separation preventing portion 445 is formed so that the fixing member 440 does not separate.

The separation preventing portion 445 is formed to protrude in a direction away from the inner wall of the lower end of the fixed inlet portion 444 .

The lower portion of the fixing body 443 is formed to coincide with the lower surface of the housing body 210 so that the fixing member 440 does not protrude to the outside when the fixing member 440 rises and enters the fixing inlet 444 .

In addition, the upper portion of the fixing body 443 is a separation prevention engaging portion 446 to be hooked on the upper end of the separation preventing portion 445 so that the fixing member 440 does not separate from the housing body 210 when descending. is formed

The separation preventing locking portion 446 is formed to protrude at a predetermined interval in a direction away from the outer surface of the fixed body 443 .

Hereinafter, the centrifugation process according to the second embodiment will be described in detail as follows.

In a state in which the main tube 20 and the membrane centrifugal concentrator 40 according to the present invention are coupled, a mixed sample to be separated is filled inside, and the tube cap 30 is coupled to the upper part of the main tube 20 to seal it. When the combined centrifuge tube 1 is rotated at a high speed at a predetermined speed, the mixed sample is separated into a first sample 2 and a second sample 3 due to a difference in centrifugal force and specific gravity.

When the separation of the sample is completed, the tube cap 30 is removed, the centrifuge tube filter 40 of the present invention is separated, and the first sample 2 collected inside is recovered by tilting the main tube 20 .

Then, after removing the sliding sealing material 430 of the membrane centrifugal concentrator 40, the stationary magnet 441 and the body magnet 442 are magnetically coupled so that the fixing member 440 is lifted and fixed, and the collecting body The 310 slides open, so that the second sample 3 can be recovered with tweezers.

The present invention described above has an advantage that the second sample 3 can be more easily recovered by forming the collecting body 310 to be separated from the housing body 210 .

Meanwhile, FIGS. 6 to 7 are partial cross-sectional views according to a third embodiment of the present invention.

The collecting unit 300 of this embodiment rotates with respect to the lower end of the housing body 210 so that the inner space of the collecting unit 300 is exposed to the outside through the rotating unit 500 through the housing unit 200. is connected to

The rotating unit 500 is provided with a rotating member 510, a rotating sealing material 520, and a Velcro unit 530 formed so that the collecting unit 300 is rotated to open the upper portion of the collecting body 310.

The rotating member 510 is installed on a corresponding surface of the housing body 210 and the collecting body 310 , and is formed to rotate the collecting body 310 . The rotation member 510 may be applied to a conventional technology capable of rotation, but as shown in the drawings, a hinge shape by a hinge shaft is preferable.

The rotating sealing material 520 is formed along the corresponding surface of the housing body 210 and the collecting body 310 so that the collecting body 310 and the housing body 210 are airtight during centrifugation by the centrifugal separator. do.

In addition, various conventional techniques may be used for the rotating sealing material 520 so that the collecting space 320 is hermetically sealed. However, an O-ring formed of a Teflon material that can improve the sealing force of the collecting unit 300 and effectively prevent leakage of the sample is preferable.

The velcro part 530 includes a first velcro 531 formed on the lower surface of the housing body 210 and the first velcro 531 formed on the outer surface of the collecting body 310 so that the collecting body 310 is not opened during centrifugation. A second velcro 532 formed so that the collecting body 310 is fixed by fastening with one velcro 531 is formed.

In addition, although not shown in the drawings, the Velcro part 530 may be in the form of a Snap Button or a Clip.

Hereinafter, the centrifugal separation process according to the third embodiment of the present invention will be described in detail as follows.

In a state in which the main tube 20 and the membrane centrifugal concentrator 50 are coupled, a mixed sample to be separated is filled therein, and the tube cap 30 is coupled to the upper part of the main tube 20 to seal it. When the combined centrifuge tube (1) is rotated at a predetermined speed, the mixed sample is separated into a first sample (2) and a second sample (3) due to a difference in centrifugal force and specific gravity.

When the separation of the sample is completed, the tube cap 30 is removed and the membrane centrifugal concentrator 50 is separated, and the first sample 2 collected inside is recovered by tilting the main tube 20 .

Subsequently, after the first velcro 531 is separated from the second velcro 532 in the membrane centrifugal concentrator 50, the collecting body 310 is rotated by the rotating member 510 while the collecting body ( The upper part of the 310 is opened, and the second sample 3 is separated by gravity and recovered in the collection space 320 .

The above-described membrane centrifugal concentrator 50 of the present invention is equipped with the rotation unit 500 to recover a small amount of the second sample 3 by washing with tweezers or distilled water, etc., so that the physical and chemical properties of the mixed sample This change can be prevented, so that the accuracy and reliability of the test can be improved.

Meanwhile, FIG. 8 is a cross-sectional view showing a fourth embodiment of the present invention.

A pressurizing unit 600 formed to guide and pressurize the mixed sample from the inlet 100 to the housing body 210 is provided.

The pressure unit 600 is introduced through the upper portion of the inlet 100 and formed to press the mixed sample, the pressure plate 610, the pressure sealing material 620, the holding rod 630, the bending member 640, the bending connection A member 650 is provided.

The pressure plate 610 is formed in the form of a thin plate so as to be able to move up and down inside the inlet part 100 . And the pressure plate 610 is a first pressure plate formed to correspond to the inner upper end of the housing body 210 so that the mixed sample accommodated in the housing body 210 does not flow back into the inlet 100 during centrifugation. 611 and a second pressure plate 612 formed along an outer extension direction of the first pressure plate 611 .

Since the pressure plate 610 is in direct contact with the mixed sample, it is preferable to be formed of a chemically very stable material that does not chemically react with the mixed sample so as not to change the characteristics of the mixed sample.

The pressure sealing material 620 is formed along the inner side of the inlet portion 100 and the contact surface of the pressure plate 610 so that the mixed sample does not remain inside the inlet portion 100 .

In addition, the pressure sealing material 620 is formed along the lower surface of the second pressure plate 612 so that the pressure plate 610 descends in the direction of the centrifugal force and is in close contact with the inner lower end of the inlet part 100 ( 621, and a sealing side portion 622 formed along the outer extended surface is provided.

The holding rod 630 is fixed inside the housing body 210 so that the pressure plate 610 moves in the direction of the centrifugal force, and penetrates the pressure plate 610 up and down to guide the lifting and lowering of the pressure plate 610 . is concluded

Although not shown in the drawings, a sealing material is further provided at the fastening portion so that the holding rod 630 and the pressure plate 610 are watertight when fastened.

In addition, the holding rod 630 may be formed of various materials that do not affect the mixed sample, but a cylindrical shape of a Teflon material is preferable.

The bending member 640 is formed to be bent at a predetermined angle with respect to the first pressure plate 611 along the inner surface of the housing body 210 . The bending member 640 may be formed of various materials that can be bent, and a plate spring having elasticity is preferable.

In addition, the formation position of the bending member 640 is not limited to the upper end of the pressing plate 610 as shown in the drawings, and is formed to be bent at various positions such as the inner side.

The bending connecting member 650 is formed on the contact surface of the bending member 640 such that one end of the bending member 640 is connected to the first pressing plate 611 and the other end is connected to the second pressing plate 612 .

The bending connecting member 650 can be used in various conventional techniques for connecting the pressing plate 610 and the bending member 640, but it is preferable that a material having an adhesive force is applied and attached thereto.

The above-described membrane centrifugal concentrator 60 of the present invention has a pressure plate 610 that can be lifted up and down inside the inlet 100 to pressurize the mixed sample, and thus pressurize the mixed sample during centrifugation. Therefore, it is effective to increase the effect of centrifugation.

The description of the presented embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the scope of the invention. Thus, the present invention is not to be limited to the embodiments presented herein, but is to be construed in the widest scope consistent with the principles and novel features presented herein.

10: filter for centrifuge tube 20: main tube
30: tube cap 100: inlet
110: locking part 200: housing part
210: housing body 220: filtering body
221: filtering hole 222: guide
223: filter fixing projection 230: filter paper
300: collecting unit 310: collecting body
320: collection space 400: sliding unit
410: first sliding member 411: coupling groove
420: second sliding member 421: coupling protrusion
430: sliding sealing material 431: inlet part
432: blocking part 440: fixing member
441: fixed magnet 442: body magnet
443: fixed body 444: fixed inlet
445: separation prevention part 446: separation prevention stopping part
500: rotation unit 510: rotation member
520: rotating sealing material 530: Velcro part
531: the first velcro 532: the second velcro
600: pressure unit 610: pressure plate
611: first pressure plate 612: second pressure plate
620: pressure sealing material 621: sealing lower part
622: sealing side part 630: holding rod
640: bending member 650: bending connecting member

Claims (7)

An inlet part having a hollow top with an open top so that the mixed sample is introduced into the inside; and
and a housing formed at a lower portion of the inlet to communicate with the inside of the inlet.
The housing portion includes a housing body having a hollow shape in which the cross section is narrowed along the direction of centrifugal force applied during centrifugation;
and a filtering body formed so as to be able to mount a filter paper on one side of the housing body,
The filtration body is characterized in that a plurality of filtration holes are formed to penetrate the inner and outer peripheral surfaces on one side so that the first sample that has passed through the filter paper can be discharged to the outside,
Membrane Centrifuge Concentrator. According to claim 1,
It further includes; a collecting part formed in the lower part of the housing body to communicate with the inner space of the housing body, and to collect the second sample remaining without passing through the filter paper.
The collecting part is characterized in that the upper part is opened and communicated with the inner space of the housing part, and it comprises a collecting body having a shape that becomes narrower as it extends in the direction of the centrifugal force,
Membrane Centrifuge Concentrator. 3. The method of claim 2,
The filtration body is provided on the inner surface and characterized in that it includes a guide at one end connected to the filtration hole so that the first sample passing through the filter paper can be guided to the filtration hole,
Membrane Centrifuge Concentrator. 4. The method of claim 3,
and a sliding unit formed such that the collecting unit moves along the upper surface of the collecting body to open a collecting space in which the second sample is collected in the collecting unit.
The sliding unit includes a first sliding member in which a coupling groove is formed in the housing body along an upper surface of the collecting body;
a second sliding member provided with a coupling protrusion so as to be engaged with the coupling groove along the formation direction of the coupling recess on the collecting body so that the collecting body slides on the first sliding member;
A sliding sealing material having an L-shaped cross section filling a gap that occurs after the coupling protrusion and the coupling groove are fastened so that the collecting space is hermetically sealed;
a fixing member formed so as to be able to ascend and descend on the housing body, and contact the rear of the collecting body when descending, and block the collecting body from sliding in an open direction;
a fixing magnet formed on the upper end of the fixing member;
When the fixing member is raised, it is provided in a contact portion that comes into contact with the housing body, characterized in that it is provided with a body magnet coupled to the fixing magnet and magnetic force so as to maintain the raised state of the fixing member,
Membrane Centrifuge Concentrator. 4. The method of claim 3,
and a rotation unit formed so that the collecting part is rotated to open the upper part of the collecting body.
The rotation unit includes a rotation member installed on a corresponding surface of the housing body and the collection body, and formed to rotate the collection body;
A rotating sealing material formed along the corresponding surfaces of the housing body and the collecting body so that the collecting body and the housing body are airtight during centrifugation by the centrifugal separator;
It includes a Velcro part so that the collecting body is not opened during centrifugation,
The velcro portion and a first velcro formed on the lower surface of the housing body,
Characterized in that the second velcro formed so that the collecting body is fixed to the outer surface of the collecting body by fastening with the first velcro,
Membrane Centrifuge Concentrator. 4. The method of claim 3,
and a pressurizing unit which is introduced through the upper part of the inlet to pressurize the mixed sample.
The pressure unit includes a pressure plate formed so as to be able to move up and down inside the inlet;
a pressure sealing material formed on a contact surface between the inside of the inlet and the pressure plate so that the mixed sample does not remain inside the inlet;
and a holding rod fixed to the inside of the housing body so that the pressing plate moves in the direction of the centrifugal force, and guiding the elevation of the pressing plate,
The holding rod is characterized in that it is fastened to penetrate the pressure plate up and down,
Membrane Centrifuge Concentrator. 7. The method of claim 6,
The pressure plate includes a first pressure plate formed to correspond to the inner upper end of the housing body so that the mixed sample accommodated in the housing body does not flow back into the inlet during centrifugation;
a second pressure plate formed along an outer extension direction of the first pressure plate and provided with a bending member to be bent at a predetermined angle with respect to the first pressure plate along an inner surface of the housing body;
and a bending connecting member formed on a lower surface of the bending member such that one end of the bending member is connected to the first pressing plate and the other end is connected to the second pressing plate,
Membrane Centrifuge Concentrator.

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