CN113398654B - Micro-centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method - Google Patents
Micro-centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method Download PDFInfo
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- CN113398654B CN113398654B CN202110575309.3A CN202110575309A CN113398654B CN 113398654 B CN113398654 B CN 113398654B CN 202110575309 A CN202110575309 A CN 202110575309A CN 113398654 B CN113398654 B CN 113398654B
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- centrifugal
- cavity
- filtrate
- filter
- centrifuge tube
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- 238000001914 filtration Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000005192 partition Methods 0.000 title claims abstract description 13
- 239000002245 particle Substances 0.000 claims abstract description 62
- 239000000706 filtrate Substances 0.000 claims abstract description 34
- 239000011521 glass Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 4
- 239000013049 sediment Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims 2
- 239000007788 liquid Substances 0.000 abstract description 20
- 239000002244 precipitate Substances 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005245 sintering Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
- B01D36/04—Combinations of filters with settling tanks
- B01D36/045—Combination of filters with centrifugal separation devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/15—Filters with filtering elements which move during the filtering operation with rotary plane filtering surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D37/00—Processes of filtration
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/38—Treatment of water, waste water, or sewage by centrifugal separation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Mechanical Engineering (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention relates to a micro-centrifugal filter pipe with a built-in vertical partition board and a centrifugal filtering method. The centrifugal filtration method comprises the following steps: 1) Adding the solution to be separated into a cavity to be filtered in the centrifuge tube; 2) Placing the centrifugal filter pipe in a centrifugal machine, and starting the centrifugal machine to perform centrifugal filtration; 3) And after centrifugal filtration is finished, sucking the filtrate in the filtrate cavity, and pouring out the residual precipitated particles in the cavity to be filtered. Compared with the prior art, the centrifugal filter pipe can be matched with a conventional centrifugal machine to be conveniently used, integrates a centrifugal function and a filtering function, can effectively separate clear liquid and precipitate particles, and is convenient for absorbing the separated clear liquid subsequently.
Description
Technical Field
The invention belongs to the technical field of solid-liquid separation, and relates to a micro-centrifugal filter tube with a built-in vertical partition plate and a centrifugal filter method.
Background
Currently, conventional techniques for separating liquids and precipitating particulates in the laboratory include centrifugation and filtration techniques.
The centrifugal technology utilizes the strong centrifugal force generated when the object rotates at high speed to make suspended particles in the rotating body subside or float, so that some particles can be concentrated or separated from other particles. Suspended particles herein are often referred to as cells, organelles, viruses, and biological macromolecules, etc., which are made into suspension. When the centrifuge rotor rotates at a high speed, when the density of suspended particles is greater than that of surrounding media, the particles move away from the axis direction and are settled; if the particle density is lower than that of the surrounding medium, the particles move toward the axial direction and float. Centrifugal techniques often employ centrifuge tubes, which are tubular specimen containers, with either a sealing cap or a gland. After centrifugation, the solid particles and liquid in the centrifuge tube are separated.
Filtration technology is the operation of separating solids and other substances from liquids (or gases) by pushing or other external forces through a medium, where the liquids (or gases) in suspension (or containing solid particles) are retained by the filter medium. Filtration techniques often use a filter plate through which solid particles and liquid are separated.
At present, when suspended particles in liquid are separated, centrifugal technology or filtering technology is often used singly, and the following defects exist: (1) When using centrifugation techniques, the centrifuged liquid and the sediment particles are not well layered or separated in the centrifuge tube, and more or less sediment particles are sucked up to the lower layer when sucking up the upper layer liquid. (2) The filtration technology often requires the use of other external power to improve the filtration effect, such as vacuum pump filtration, etc., which requires high demands on the device.
In order to solve the above problems, in the prior art, a centrifugal technique and a filtering technique are combined together, and a flow path with an upper structure and a lower structure is designed by adopting a sleeve structure so as to promote solid-liquid separation (for example, a high-speed centrifugal filter tube, a centrifugal filter tube and a centrifugal filter tube, which are disclosed in the publication No. CN205659797U, the publication No. CN204182492U and the publication No. CN 205253381U). However, such structures and flow paths cannot withstand large centrifugal forces, and therefore, the separation and filtration effects are often not ideal.
Disclosure of Invention
The invention aims to provide a micro-centrifugal filter pipe with a built-in vertical partition board and a centrifugal filtering method. The centrifugal filter tube can be conveniently used in combination with a conventional centrifugal machine, integrates a centrifugal function and a filtering function, can effectively separate clear liquid and precipitated particles, and is convenient for absorbing the separated clear liquid subsequently.
The aim of the invention can be achieved by the following technical scheme:
the utility model provides a micro-centrifugal filter tube of built-in vertical baffle, this centrifugal filter tube includes the centrifuging tube body of vertical setting and vertical setting at the inside filter of centrifuging tube, is equipped with the filtration pore on this filter, the inside of filter with the centrifuging tube body be waiting to filter chamber and filtrate chamber, the inside of waiting to filter chamber be equipped with the granule supporting shoe. During centrifugal filtration, liquid in the solution to be separated in the cavity to be filtered passes through the filter holes and enters the filtrate cavity, and residual precipitated particles remain in the cavity to be filtered, so that solid-liquid separation is realized. Meanwhile, as the particle supporting blocks are arranged in the cavities to be filtered, the residual precipitated particles are all located on the particle supporting blocks, and the residual precipitated particles are lifted through the particle supporting blocks, so that the residual precipitated particles are prevented from being contacted and mixed with filtrate on the other side of the filter plate again. The particle support block is of a solid structure, so that liquid infiltration is avoided.
Further, the top of the centrifuge tube body is provided with an opening. The solution to be separated is added to the chamber to be filtered through the top opening of the centrifuge tube.
Further, the top opening of the centrifuge tube body is detachably provided with a sealing cover.
Further, a plurality of filter holes are uniformly formed in the filter plate, and the aperture of each filter hole is in a micron level or a millimeter level. The filter plate is connected with the side surface and the bottom of the centrifuge tube body.
Further, the bottom of the particle supporting block is connected with the bottom of the centrifuge tube body, and the side face of the particle supporting block is connected with the side face of the centrifuge tube body and the side face of the filter plate respectively.
Further, the top surface of the particle support block is higher than the bottom of the filtrate cavity.
Further, the centrifuge tube body, the filter plate and the particle supporting block are all made of glass. The glass material is easy to clean and can be reused, and the glass material is easy to obtain and low in cost.
A centrifugal filtration method based on the micro-centrifugal filtration tube with the built-in vertical partition plate, which comprises the following steps:
1) Adding the solution to be separated into a cavity to be filtered in the centrifuge tube;
2) Placing the centrifugal filter pipe in a centrifugal machine, and starting the centrifugal machine to perform centrifugal filtration;
3) And after centrifugal filtration is finished, sucking the filtrate in the filtrate cavity, and pouring out the residual precipitated particles in the cavity to be filtered.
Further, in step 3), the filtrate in the filtrate chamber is sucked up by a pipette or syringe.
Further, in step 3), after the centrifugal filtration is finished, the top surface of the particle support block is higher than the top surface of the filtrate in the filtrate cavity, and the residual precipitated particles in the filtrate cavity to be filtered are positioned on the particle support block.
Compared with the prior art, the invention has the following characteristics:
1) The centrifugal filter pipe integrates a centrifugal function and a filtering function, can effectively separate clear liquid and precipitate particles, and is convenient for absorbing the separated clear liquid subsequently.
2) The inside of the centrifuge tube body is vertically provided with the filter plate, the flow path of the upper structure and the lower structure in the prior art is changed into a left-right structure, the centrifuge tube body can bear larger centrifugal force, meanwhile, through the arrangement of the particle supporting block in the inside of the filter cavity to be filtered, the re-contact mixing of the filtered sediment particles and the filtrate on the other side of the filter plate is avoided, and the centrifuge tube has an excellent filtering effect.
Drawings
FIG. 1 is a schematic view of a schematic cross-sectional front view of a centrifugal filter tube in accordance with the present invention;
FIG. 2 is a schematic top view of a centrifugal filter tube (with particle support blocks omitted) according to the present invention;
the figure indicates:
1-centrifuge tube, 2-filter plate, 3-filter cavity, 4-filtrate cavity, 5-particle supporting block, 6-filtrate and 7-precipitated particles.
Detailed Description
The invention will now be described in detail with reference to the drawings and specific examples. The present embodiment is implemented on the premise of the technical scheme of the present invention, and a detailed implementation manner and a specific operation process are given, but the protection scope of the present invention is not limited to the following examples.
Examples:
the micro-centrifugal filter tube with the built-in vertical partition plates is shown in fig. 1 and 2, and comprises a centrifuge tube body 1 which is vertically arranged and a filter plate 2 which is vertically arranged in the centrifuge tube body 1, wherein filter holes are formed in the filter plate 2, the filter plate 2 divides the inner part of the centrifuge tube body 1 into a to-be-filtered cavity 3 and a filtrate cavity 4, and a particle supporting block 5 is arranged in the to-be-filtered cavity 3.
Wherein, the top of centrifuging tube body 1 is equipped with the opening. The top opening of the centrifuge tube body 1 is detachably provided with a sealing cover.
The filter plate 2 is uniformly provided with a plurality of filter holes, and the aperture of each filter hole is in a micron level or a millimeter level. The bottom of the particle supporting block 5 is connected with the bottom of the centrifuge tube body 1, and the side surface of the particle supporting block 5 is respectively connected with the side surface of the centrifuge tube body 1 and the side surface of the filter plate 2. The top surface of the pellet support block 5 is higher than the bottom of the filtrate chamber 4. The centrifuge tube body 1, the filter plate 2 and the particle supporting block 5 are all made of glass.
The centrifugal filtration method based on the centrifugal filtration pipe comprises the following steps:
(1) Preparing a centrifuge tube body 1 with a proper tube diameter, such as a volume of 20mL, 50mL, 100mL and the like, according to the capacity of the centrifuge; the filter plate 2 is connected with proper sintering according to the particle size of the particles to be centrifuged and filtered, and the pore diameter can be in micron or millimeter level.
(2) The solution to be separated is added into the cavity to be filtered 3 in the centrifuge tube body 1, and the liquid level cannot exceed the height of the filter plate 2 so as to prevent the solution to be separated from overflowing the filter plate 2.
(3) Placing the centrifugal filter tube in a centrifuge, starting the centrifuge to perform centrifugal filtration, and selecting proper rotation speed according to the properties of the solution to be separated, such as 800r/min, 1000r/min, 1200r/min and the like; the centrifugation time is 20min, 30min, 40min, etc.
(4) After centrifugal filtration is finished, the top surface of the particle support block 5 is higher than the top surface of the filtrate 6 in the filtrate cavity 4, and the residual precipitated particles 7 in the filtrate cavity 3 are all positioned on the particle support block 5. The filtrate 6 in the filtrate chamber 4 is sucked up by means of a pipette or syringe to be subjected to subsequent test analysis.
(5) And pouring out the residual precipitated particles 7 in the cavity 3 to be filtered, cleaning the centrifugal filter tube, and airing for later use.
The previous description of the embodiments is provided to facilitate a person of ordinary skill in the art in order to make and use the present invention. It will be apparent to those skilled in the art that various modifications can be readily made to these embodiments and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments, and those skilled in the art, based on the present disclosure, should make improvements and modifications without departing from the scope of the present invention.
Claims (7)
1. The micro-centrifugal filter tube with the built-in vertical partition plates is characterized by comprising a centrifuge tube body (1) which is vertically arranged and a filter plate (2) which is vertically arranged in the centrifuge tube body (1), wherein filter holes are formed in the filter plate (2), the filter plate (2) divides the inner part of the centrifuge tube body (1) into a to-be-filtered cavity (3) and a filtrate cavity (4), and a particle supporting block (5) is arranged in the to-be-filtered cavity (3);
a plurality of filter holes are uniformly formed in the filter plate (2), and the aperture of each filter hole is in a micron level or a millimeter level;
the top surface of the particle supporting block (5) is higher than the bottom of the filtrate cavity (4);
the bottom of the particle supporting block (5) is connected with the bottom of the centrifuge tube body (1), and the side surface of the particle supporting block (5) is respectively connected with the side surface of the centrifuge tube body (1) and the side surface of the filter plate (2);
after centrifugal filtration is finished, the top surface of the particle support block (5) is higher than the top surface of the filtrate (6) in the filtrate cavity (4), and the residual precipitation particles (7) in the to-be-filtered cavity (3) are positioned on the particle support block (5), so that the filtered precipitation particles are prevented from being contacted and mixed with the filtrate on the other side of the filter plate (2) again.
2. The micro-centrifugal filter tube with the built-in vertical partition plate according to claim 1, wherein an opening is arranged at the top of the centrifuge tube body (1).
3. The micro-centrifugal filter tube with the built-in vertical partition plate according to claim 2, wherein a sealing cover is detachably arranged at the top opening of the centrifuge tube body (1).
4. The micro-centrifugal filter tube with the built-in vertical partition plate according to claim 1, wherein the centrifuge tube body (1), the filter plate (2) and the particle supporting block (5) are all made of glass.
5. A centrifugal filtration method based on the micro centrifugal filtration tube with built-in vertical partition plate according to any one of claims 1 to 4, characterized in that the method comprises the steps of:
1) Adding the solution to be separated into a to-be-filtered cavity (3) in the centrifuge tube body (1);
2) Placing the centrifugal filter pipe in a centrifugal machine, and starting the centrifugal machine to perform centrifugal filtration;
3) After centrifugal filtration is finished, the filtrate (6) in the filtrate cavity (4) is sucked, and then the residual sediment particles (7) in the cavity (3) to be filtered are poured out.
6. The centrifugal filtration method of a micro centrifugal filtration tube based on a built-in vertical separator according to claim 5, wherein in step 3), the filtrate (6) in the filtrate chamber (4) is sucked up by a pipette or a syringe.
7. The centrifugal filtration method of the micro-centrifugal filtration tube based on the built-in vertical partition plate according to claim 5, wherein in the step 3), after the centrifugal filtration is finished, the top surface of the particle supporting block (5) is higher than the top surface of the filtrate (6) in the filtrate cavity (4), and the residual precipitated particles (7) in the cavity (3) to be filtered are positioned on the particle supporting block (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110575309.3A CN113398654B (en) | 2021-05-26 | 2021-05-26 | Micro-centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110575309.3A CN113398654B (en) | 2021-05-26 | 2021-05-26 | Micro-centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method |
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| Publication Number | Publication Date |
|---|---|
| CN113398654A CN113398654A (en) | 2021-09-17 |
| CN113398654B true CN113398654B (en) | 2023-11-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110575309.3A Active CN113398654B (en) | 2021-05-26 | 2021-05-26 | Micro-centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207770061U (en) * | 2017-11-28 | 2018-08-28 | 贵州开磷质量检测中心有限责任公司 | A kind of double-deck centrifuge tube for solid-liquid quick separating |
| CN109794366A (en) * | 2019-01-14 | 2019-05-24 | 上海化工研究院有限公司 | A kind of flexible type micro high speed centrifugation device of umbrella shape |
| CN111408428A (en) * | 2020-05-02 | 2020-07-14 | 南京大学 | Double-cavity centrifuge tube integrating functions of suspension separation and supernatant extraction |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170001203A1 (en) * | 2015-07-03 | 2017-01-05 | Seven Juice Co., Ltd. | Centrifugal filtering device and method for operating the same |
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- 2021-05-26 CN CN202110575309.3A patent/CN113398654B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207770061U (en) * | 2017-11-28 | 2018-08-28 | 贵州开磷质量检测中心有限责任公司 | A kind of double-deck centrifuge tube for solid-liquid quick separating |
| CN109794366A (en) * | 2019-01-14 | 2019-05-24 | 上海化工研究院有限公司 | A kind of flexible type micro high speed centrifugation device of umbrella shape |
| CN111408428A (en) * | 2020-05-02 | 2020-07-14 | 南京大学 | Double-cavity centrifuge tube integrating functions of suspension separation and supernatant extraction |
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| Publication number | Publication date |
|---|---|
| CN113398654A (en) | 2021-09-17 |
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