CN215262726U - Filter and negative pressure balance centrifugal bottle capable of preventing overflow - Google Patents

Abstract

The utility model provides a filter, which comprises a filter body, wherein the filter body is of a structure with two circumferentially closed ends and two openings, and the upper part of the filter body is provided with a liquid inlet; the outer surface of the filter body is provided with an annular plate, and the lower part of the filter body is used for installing a filter membrane. Furthermore, the utility model also provides a prevent balanced centrifugal flask of negative pressure of overflow, including above-mentioned filter, centrifugal flask body, bottle lid, the filter sets up this is internal for the centrifugal flask, bottle lid detachably sets up on the centrifugal flask body, the outer wall of filter body the annular slab with the inner wall of centrifugal flask body encloses synthetic annular overflow launder. The utility model discloses with the filter setting this internally at the centrifuge bottle, the outer wall of filter body, annular slab and the inner wall of centrifuge bottle body encloses synthetic annular overflow launder, can prevent that the liquid in the centrifuge bottle from overflowing outside the bottle, still can carry out multistage filtration.

Description

Filter and negative pressure balance centrifugal bottle capable of preventing overflow

Technical Field

The utility model relates to a cell film-making equipment technical field specifically relates to a filter and prevent negative pressure balance centrifugal bottle of overflow.

Background

For some exfoliative cytological specimens with high impurity content and complex components, such as cyst puncture fluid, stool specimen, etc., at present, a method is known, which firstly carries out multi-stage filtration, centrifuges the obtained filtrate, then carries out specific gravity separation, cell concentration, processes the cell concentrated solution with a small amount of cell treatment solution to obtain high-concentration cell suspension, and finally adds the cell suspension into a slide making bin for making a slide. Therefore, the negative pressure balance centrifugal bottle is produced by transportation, during the flaking, the negative pressure balance centrifugal bottle is clamped on the flaking bin to form a centrifugal kit, the centrifugal kit is installed on the centrifugal machine, the centrifugal machine can effectively complete the whole processes of specific gravity separation, cell concentration and flaking of the cell specimen liquid through multiple times of low-speed and high-speed circulating rotation, and the problem of cell loss caused by multiple times of cell specimen liquid transfer is effectively avoided.

However, in the process of slicing by using the existing negative pressure balance centrifuge bottle, in order to separate impurities, a filter membrane must be additionally arranged in the centrifuge bottle, in order to ensure the filtering effect, the diameter of the filter membrane cannot be too small, which requires that the diameter of the centrifuge bottle cannot be too small, liquid in the centrifuge bottle can flow into a slicing bin under the action of centrifugal force, under the condition of a certain rotating speed, the larger the air space in the centrifuge bottle after being filled with sample liquid is, the more liquid flows into the slicing bin, in order to increase the rotating speed and not to cause the excessive liquid flowing into the slicing bin to overflow, the more sample liquid must be filled as much as possible to reduce the air space in the centrifuge bottle, and the excessive sample filling liquid easily causes the sample liquid to overflow to the outside of the centrifuge bottle to cause pollution.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solving one of the technical problem that exists among the prior art at least. Therefore, the utility model provides a filter, include:

the filter body (2) is of a structure with two circumferentially closed ends opened, and a liquid inlet (201) is formed in the upper part of the filter body (2); the outer surface of the filter body (2) is provided with an annular plate (202), and the lower part of the filter body (2) is used for installing a filter membrane.

Further, the upper surface of the annular plate (202) is provided with an annular groove (211).

Further, the upper portion of filter body (2) is sample liquid chamber (206), the lower part of filter body (2) is processing liquid chamber (207), communicate through pore (205) between sample liquid chamber (206) and the processing liquid chamber (207).

Further, the pore diameter of the fine pore (205) is 1 to 3 mm.

Further, a first filtering membrane (208) is arranged in the treatment liquid cavity (207).

Furthermore, a second filtering membrane (209) is also arranged in the treatment liquid cavity (207).

Furthermore, the utility model also provides a prevent balanced centrifugal flask of negative pressure of overflow, including above-mentioned arbitrary filter, centrifugal flask body (1), bottle lid (101), the filter sets up in centrifugal flask body (1), bottle lid (101) detachably sets up on the centrifugal flask body (1), the outer wall of filter body (2) annular board (202) with the inner wall of centrifugal flask body (1) encloses and closes annular overflow launder (203).

Further, the inner wall of the top of the bottle cap (101) is attached to the liquid inlet (201), and a first sealing ring (204) is arranged between the annular plate (202) and the inner wall of the centrifuge bottle body (1).

Further, be provided with conical body (104) on centrifuge bottle body (1), be provided with tiny opening (102) on conical body (104), the tip of tiny opening (102) is blind end (105), still includes joint portion (3), joint portion (3) set up the surface of centrifuge bottle body (1).

The utility model discloses with the filter setting at this internally of centrifuge bottle, the outer wall of filter body, annular slab and centrifuge bottle's inner wall enclose synthetic annular overflow launder, can prevent that the liquid in the centrifuge bottle from overflowing outside the bottle, still can carry out multistage filtration.

Drawings

FIG. 1 is a schematic structural view of a negative pressure balance centrifuge bottle for preventing overflow provided by the present invention;

fig. 2 is a schematic structural diagram of the filter provided by the present invention;

FIG. 3 is a schematic structural view of the negative pressure balancing centrifuge bottle filled with the treating fluid;

FIG. 4 is a schematic structural view of the storage or transportation state of the negative pressure balance centrifuge bottle filled with the treatment liquid according to the present invention;

fig. 5 is a schematic structural diagram of the present invention after pouring the processing liquid in the specimen liquid chamber;

fig. 6 is a schematic structural diagram of the operation of the negative pressure balance centrifuge bottle for preventing overflow in the centrifuge provided by the present invention;

wherein, 1, centrifuging the bottle body; 101. a bottle cap; 102. a fine opening; 103. a thin tube; 104. a conical body; 105. a blind end; 2. a filter body; 201. a liquid inlet; 202. an annular plate; 203. an annular overflow trough; 204. a first sealing ring; 205. fine pores; 206. a specimen fluid chamber; 207. a treatment fluid chamber; 208. a first filtering membrane; 209. a second filtering membrane; 210. a second sealing ring; 211. an annular groove; 3. a clamping part; 4. a treatment liquid; 5. a cell sample solution; 6. a tablet making bin; 7. a slide rack; 8. a glass slide; 9. a centrifugal shaft; 901. a centrifugal arm; 902. a hanging basket.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected" and "disposed" are to be construed broadly, and may for example be fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in fig. 1 and 2, the utility model provides a negative pressure balance centrifugal bottle for preventing overflow, which comprises: filter, centrifuge bottle body 1, bottle lid 101.

The filter comprises a filter body 2, wherein the filter body 2 is of a structure with two circumferentially closed ends opened, and a liquid inlet 201 is formed in the upper part of the filter body 2; an annular plate 202 is arranged on the outer surface of the filter body 2 and is close to the liquid inlet, and a filter membrane is arranged at the lower part of the filter body 2; the upper surface of the annular plate 202 can be a plane structure and can also be provided with an annular groove 211; the upper part of the filter body 2 is a sample liquid cavity 206, the lower part of the filter body 2 is a treatment liquid cavity 207, the sample liquid cavity 206 is communicated with the treatment liquid cavity 207 through a fine hole 205, and the aperture of the fine hole 205 is 1-3 mm; and a first filtering membrane 208 and a second filtering membrane 209 are arranged in the treatment liquid cavity 207, the second filtering membrane is positioned below the first filtering membrane, and the aperture of the filtering hole on the second filtering membrane is smaller than that of the filtering hole on the first filtering membrane.

The filter is arranged in the centrifuge bottle body 1, the bottle cap 101 is detachably arranged on the centrifuge bottle body 1, an annular overflow groove 203 is defined by the outer wall of the filter body 2, the annular plate 202 and the inner wall of the centrifuge bottle body 1, the inner wall of the top of the bottle cap 101 is attached to the liquid inlet 201, no seal is formed between the bottle cap and the liquid inlet, and a first seal ring 204 is arranged between the side surface of the annular plate 202 and the inner wall of the centrifuge bottle body 1. Specifically, the sealing ring is sleeved on the side surface of the annular plate, and when the filter is installed in the centrifuge bottle, the first sealing ring is tightly attached to the inner wall of the centrifuge bottle body; the centrifugal filter comprises a filter body and a second sealing ring 210, wherein the second sealing ring is sleeved on the outer surface of the filter body close to the second filter membrane, and when the filter is installed in a centrifugal bottle, the second sealing ring is tightly attached to the inner wall of the centrifugal bottle body.

One end of the centrifuge bottle body 1 is provided with tiny opening 102, tiny opening 102 with be provided with between the centrifuge bottle body 1 with tubule 103 and conical body 104 that tiny opening 102 links to each other, the main aspects of conical body 104 with centrifuge bottle body 1 links to each other, the tip of conical body 104 with tubule 103 links to each other, the internal diameter of tubule 103 can with tiny opening 102's internal diameter is the same, tiny opening 102's internal diameter is 1 ~ 3 mm. Specifically, the end of the fine opening 102 is a blind end 105 of plastic or the like, which is easily cut off, and the blind end is cut off to expose the fine opening during centrifugal sheet production.

Still include joint portion 3, joint portion 3 sets up in tubule 103, centrifuge bottle body 1 or conical body 104 the outward appearance all can for cooperate so that fix a position, support the centrifuge bottle in film-making storehouse 6 with film-making storehouse 6, joint portion 3 is for having the locating piece of convex shoulder.

The utility model discloses a theory of operation does:

when the factory leaves the factory, the treatment liquid 4 is added into the centrifuge bottle from the liquid inlet until the centrifuge bottle is full, then the bottle cap 101 is covered on the centrifuge bottle body 1, and the centrifuge bottle full of the treatment liquid is shown in figure 3. Fig. 4 is a structural diagram of a storage or transportation state of a centrifuge bottle filled with a processing liquid, the centrifuge bottle is horizontally arranged, the liquid in the specimen liquid cavity overflows to the annular overflow groove, but both ends of the pore are still in the liquid, and air in the specimen liquid cavity still cannot enter the processing liquid cavity due to the small diameter of the pore.

Before filling the sample liquid, the bottle cap needs to be opened to pour the liquid in the sample liquid cavity, the liquid overflowing from the sample liquid cavity into the annular overflow groove is also poured, and due to the action of surface tension, even in an inverted state, if the bottle cap does not shake violently, air in the sample liquid cavity cannot enter the processing liquid cavity through the fine hole, as shown in fig. 5.

The specimen liquid 5 is added into the specimen liquid cavity until the specimen liquid cavity is filled with the specimen liquid 5, the bottle cap is tightly covered, slight shaking is inevitable in the process of tightly covering the bottle cap, less liquid in the specimen liquid cavity overflows to the annular overflow groove, but the liquid in the annular overflow groove does not obviously shake under the action of liquid surface tension due to the narrow annular overflow groove, so that the specimen liquid is limited to overflow out of the centrifuge bottle, and the pollution of the specimen liquid to the outer wall of the centrifuge bottle is avoided.

Then, the adhesive-coated slide 8 is prepared and mounted in the slide holder 7. Insert the card ear on the film-making storehouse 6 in the slide frame 7, the sealing washer on the film-making storehouse 6 is hugged closely on slide 8, cut the end cover on the centrifuge bottle and expose tiny opening 102, because the effect of surface tension, liquid in the centrifuge bottle can not follow a large amount of effluences in tiny opening 102 department, later will be equipped with treatment fluid 4, the centrifuge bottle of cell specimen liquid 5 installs and forms centrifugal external member on the other end in the film-making storehouse, wherein tiny opening 102 on the centrifuge bottle towards the slide and with the slide distance between the upper surface be 0.5-15mm, because 3 locating pieces of joint portion, do not form sealing contact between centrifuge bottle and the film-making storehouse 6, the inside and external atmosphere in film-making storehouse 6 can keep unobstructed.

Next, the centrifuge kit is placed in the basket 902 of a basket centrifuge. The basket type centrifuge comprises a centrifugal shaft 9, a centrifugal arm 901 and a hanging basket 902, wherein the centrifugal arm 901 is horizontally and fixedly arranged on the centrifugal shaft 9, the hanging basket 902 is hinged to the centrifugal arm 901, the centrifuge is started, the centrifugal shaft 9 rotates to drive the centrifugal arm 901 to rotate, the hanging basket 902 is gradually close to a horizontal state under the action of centrifugal force, and as shown in fig. 6, liquid in a centrifuge bottle flows into a tablet making bin under the action of centrifugal force.

The rotating speed of the centrifuge is gradually increased to a high rotating speed and maintained for a certain time, liquid in the centrifuge bottle continuously flows into the tablet making bin under the action of centrifugal force along with the increase of the rotating speed, when the liquid in the tablet making bin flows through the fine opening, outside air cannot enter the centrifuge bottle, the space volume in the centrifuge bottle continuously increases along with the continuous outflow of the liquid, the air pressure continuously decreases along with the increase of the space volume, the negative pressure in the centrifuge bottle is gradually increased relative to the atmospheric pressure, when the centrifugal force is balanced with the negative pressure, the liquid in the centrifuge bottle does not flow into the tablet making bin any more, and when the centrifugal force is balanced with the negative pressure in the centrifuge bottle, the liquid level in the centrifuge bottle is below the filter membrane I, as shown in fig. 6; under the action of centrifugal force, the mucus block on the first filtering membrane is subjected to huge centrifugal force due to the loss of buoyancy support, and as a result, the mucus block is torn and passes through the first filtering membrane to enter a sample liquid cavity between the second filtering membrane and the first filtering membrane; due to the elastic deformation of mucus, a mucus block which is larger than the aperture of the membrane hole of the first filtering membrane can also enter a sample liquid cavity between the second filtering membrane and the first filtering membrane, but the mucus block is positioned in the liquid in the sample liquid cavity between the second filtering membrane and the first filtering membrane, the centrifugal force applied to the mucus block is very limited due to the buoyancy effect of the liquid, the larger mucus block is stopped by the second filtering membrane and stays on the second filtering membrane and blocks the membrane hole, and the diameter of cells and cell clusters is smaller than the aperture of the membrane hole of the second filtering membrane, so that part of the cells and the cell clusters enter the conical body through the second filtering membrane and further move towards the glass slide; after the high rotating speed of the centrifuge lasts for a certain time, the second filtering membrane is blocked by the mucus block, a large number of cells are also left in the sample liquid cavity between the second filtering membrane and the first filtering membrane, the rotating speed of the centrifuge is reduced, the centrifuge is operated at a low rotating speed and maintained for a certain time, the centrifugal force is reduced, the negative pressure in the centrifuge bottle is large, and the liquid level in the slide making bin overflows the fine openings to form sealing.

The cells and small impurity particles filtered into the conical body continuously move towards the tubule direction of the centrifuge bottle under the action of centrifugal force, but because the specific gravity of the impurity particles is smaller than that of the cells, the movement speed of the impurity particles is slower than that of the cells, when the cells reach the outside of the tubule of the centrifuge bottle, the impurity particles still need a certain time to reach the outside of the tubule and move to the cells outside the tubule of the centrifuge bottle, and finally the cells in front of the tubule of the centrifuge bottle are pushed to the whole slide making bin through high and low rotation speed circulation of the centrifuge bottle so as to be uniformly adsorbed on a slide glass, thereby completing the three processes of filtering, specific gravity separation and cell slide at one time. Wherein, the range of the high rotation speed of the centrifuge is 200-1000 r/min, and the range of the low rotation speed is 100-900 r/min. The utility model provides a balanced centrifugal bottle of negative pressure still is suitable for handling the more sample of impurity, like cyst puncture sample and shit sample, and at this moment, the aperture of the membrane pore of filtration membrane one is great to with the characteristic phase-match of sample impurity, the film-making method is with last, adopts high low rotational speed circulation, no longer gives details here.

While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims (9)

1. A filter, comprising:

the filter body (2) is of a structure with two circumferentially closed ends opened, and a liquid inlet (201) is formed in the upper part of the filter body (2); the outer surface of the filter body (2) is provided with an annular plate (202), and the lower part of the filter body (2) is used for installing a filter membrane.

2. A filter according to claim 1, characterised in that the upper surface of the annular plate (202) is provided with an annular groove (211).

3. The filter of claim 1, wherein the upper portion of the filter body (2) is a sample liquid chamber (206), the lower portion of the filter body (2) is a processing liquid chamber (207), and the sample liquid chamber (206) and the processing liquid chamber (207) are communicated through the fine hole (205).

4. The filter according to claim 3, wherein the pores (205) have a pore size of 1 to 3 mm.

5. A filter according to claim 3, wherein a first filter membrane (208) is arranged in the treatment liquid chamber (207).

6. A filter according to claim 3, wherein a second filter membrane (209) is also arranged in the treatment liquid chamber (207).

7. A negative pressure balancing centrifuge bottle for preventing overflow, comprising a filter according to any of claims 1 to 6, a centrifuge bottle body (1), a bottle cap (101), wherein the filter is arranged in the centrifuge bottle body (1), the bottle cap (101) is detachably arranged on the centrifuge bottle body (1), and an outer wall of the filter body (2), the annular plate (202) and an inner wall of the centrifuge bottle body (1) enclose an annular overflow tank (203).

8. The negative pressure balance centrifuge bottle for preventing overflow according to claim 7, wherein the inner wall of the top of the bottle cap (101) is attached to the liquid inlet (201), and a first sealing ring (204) is arranged between the annular plate (202) and the inner wall of the centrifuge bottle body (1).

9. The negative pressure balance centrifuge bottle for preventing overflow according to claim 7, wherein the centrifuge bottle body (1) is provided with a cone (104), the cone (104) is provided with a fine opening (102), the end of the fine opening (102) is a blind end (105), and the centrifuge bottle further comprises a clamping portion (3), and the clamping portion (3) is arranged on the outer surface of the centrifuge bottle body (1).

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