CN212335187U

CN212335187U - Blood component automatic balance separator

Info

Publication number: CN212335187U
Application number: CN202022201183.7U
Authority: CN
Inventors: 李灵溪
Original assignee: Jinximeng Tianjin Artificial Intelligence Co ltd
Current assignee: Jinximeng Tianjin Artificial Intelligence Co ltd
Priority date: 2020-09-30
Filing date: 2020-09-30
Publication date: 2021-01-12
Anticipated expiration: 2030-09-30

Abstract

The utility model relates to a blood composition handles technical field, the utility model discloses a blood composition automatic balance separator, including binary channels rotary seal and single channel rotary seal, binary channels rotary seal intercommunication centrifuging tube, two effect air pumps, balancing pipe and quality controller, quality controller electric connection shock transducer, the first piston of centrifuging tube active fit, balancing pipe active fit second piston, single channel rotary seal intercommunication centrifuging tube and first three-way pipe. The utility model discloses the realization improves the separation efficiency and the goods quality of blood composition with the flow direction that the blood composition direction of difference corresponds to through vibrations sensor and quality controller, keep rotor mass distribution's balance constantly, guarantee centrifugal effect.

Description

Blood component automatic balance separator

Technical Field

The utility model relates to a blood composition handles technical field, specifically is a blood composition automatic balance separator.

Background

The current cell technology is developed rapidly, and the research and application of the cell technology still have a large space. The essential steps of the research and application related to cell technology are the acquisition of stem cells or immune cells, such as cord blood stem cells and peripheral blood mononuclear cells, and the centrifugal separation of cord blood or peripheral blood by using a centrifugal technology.

The quality and efficiency of centrifugation directly affect the results of related research work and applications. At present, a simple centrifuge and an open centrifuge tube are generally adopted as an operation method for separating blood components, and in order to reduce the risk of bacterial contamination in the operation process, an operator needs to perform complex and accurate transfer operation of the blood components and reagents under equipment such as an ultra-clean workbench. The operation process is long in time, the separation quality is difficult to guarantee, waste of blood resources is easily caused by misoperation, the risk of contamination still exists, hidden dangers are buried for application of blood components, and waste of more resources is caused.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a blood composition automatic balance separator to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a blood composition automatic balance separator, includes the frame, set firmly in motor on the frame, set firmly in the epaxial main shaft of output of motor, set firmly in epaxial rotor is the annular evenly set firmly in bucket on the rotor, insert locate in the bucket and about the main shaft is the centrifuging tube and the balancing pipe of symmetric distribution, set firmly binary channels rotary seal on the frame, the both ends difference pipe connection of a binary channels rotary seal's a passageway has the lower port and the double-acting air pump of centrifuging tube, just the both ends difference pipe connection of another passageway of binary channels rotary seal has the lower port and the quality control ware of balancing pipe, the inner wall clearance fit of centrifuging tube has first piston, the inner wall clearance fit of balancing pipe has the second piston, just be located on the centrifuging tube the port pipe connection of second piston top has single channel rotary seal, the single-channel rotary sealing pipeline is connected with a first three-way pipe, the first three-way pipe is connected with a second three-way pipe and a third three-way pipe, the second three-way pipe is connected with a blood bag and a plasma bag, and the third three-way pipe is connected with a reagent bag and a nucleated cell bag;

the bottom of frame has set firmly the damping bumper shock absorber, the bottom of damping bumper shock absorber has set firmly the frame, set firmly the vibrations sensor in the frame, the output electric connection of vibrations sensor has quality control ware's input.

Optionally, a fluid component sensor is disposed on a pipeline between the single-channel rotary seal and the first three-way pipe, and the fluid component sensor includes a blood component sensor.

Optionally, a bacterial filter is fixedly arranged at a port in the centrifugal tube above the first piston, and the bacterial filter comprises a cellulose membrane filter.

Optionally, the shock sensor comprises an inertial moving coil type electrodynamic sensor.

Optionally, the double-channel rotary seal is fixedly arranged on the frame; the frame has set firmly centrifuge cover, centrifuge cover's top articulates there is the centrifuge upper cover, centrifuge cover's top has set firmly single channel rotary seal, single channel rotary seal runs through the centrifuge upper cover.

Optionally, a fluid pressure difference sensor is arranged on the first three-way pipe; electromagnetic valves or manual valves are arranged on the second three-way pipe and the third three-way pipe.

Compared with the prior art, the utility model provides a blood composition automatic balance separator possesses following beneficial effect:

1. the utility model realizes guiding different blood components to corresponding flow directions, improves the separation efficiency of the blood components and the product quality, constantly keeps the balance of the rotor mass distribution through the vibration sensor and the quality controller, and ensures the centrifugal effect;

2. the utility model discloses a separator adopts totally closed to the process of blood separation, and no bacterial contamination risk in the operation process, the transfer easy operation and the high efficiency of blood composition and reagent through fluid composition sensor discernment blood composition, avoid leading to blood extravagant for the maloperation.

Drawings

Fig. 1 is a schematic overall structure diagram of the present invention;

FIG. 2 is a schematic structural view of a centrifuge tube according to the present invention;

fig. 3 is a schematic structural view of the leveling pipe of the present invention.

In the figure: the device comprises a motor 1, a machine base 2, a main shaft 3, a rotor 4, a bucket 5, a double-channel rotary seal 6, a single-channel rotary seal 7, a centrifuge tube 8, a balance tube 9, a damping shock absorber 10, a vibration sensor 11, a fluid pressure difference sensor 12, a centrifuge cover 13, a centrifuge upper cover 14, a machine frame 15, a first three-way pipe 16, a fluid component sensor 17, a quality controller 18, a double-acting air pump 19, a first piston 20, a second piston 21, a second three-way pipe 22, a third three-way pipe 23, a blood bag 24, a blood plasma bag 25, a reagent bag 26, a nucleated cell bag 27 and a bacterial filter 28.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example (b): referring to fig. 1, the present invention provides an automatic blood component balancing and separating device, which comprises a base 2, a motor 1 fixed on the base 2, a main shaft 3 fixed on an output shaft of the motor 1, a rotor 4 fixed on the main shaft 3, a bucket 5 uniformly fixed on the rotor 4 in an annular shape, a centrifuge tube 8 inserted into the bucket 5 and symmetrically distributed about the main shaft 3, and a balancing tube 9. The bottom of the base 2 is fixedly provided with a damping shock absorber 10, the bottom of the damping shock absorber 10 is fixedly provided with a frame 15, the frame 15 is fixedly provided with a vibration sensor 11, and the vibration sensor 11 is an inertial moving coil type electric sensor.

Two-channel rotary seal 6 is fixedly arranged on the machine base 2, two ends of one channel of the two-channel rotary seal 6 are respectively connected with a lower port of a centrifugal tube 8 and a double-acting air pump 19 through high-pressure tubes, two ends of the other channel of the two-channel rotary seal 6 are respectively connected with a lower port of a balancing tube 9 and a quality controller 18 through high-pressure tubes, and the output end of the vibration sensor 11 is electrically connected with the input end of the quality controller 18. The quality controller 18 selects a pump body with a comparator, the comparator stores parameters in advance, the vibration sensor 11 transmits a vibration signal acquired from the rack 15 to the quality controller 18, the comparator compares the vibration signal size data with the parameters, and when the vibration signal is larger than the parameter value, the pump body pumps a certain amount of liquid from the outside to balance the mass of the rotor 4; when the vibration signal is less than the parameter value, the pump body pumps a certain amount of liquid from the trim tube 9 so that the rotor 4 is mass balanced.

Referring to fig. 2, the inner wall of the centrifugal tube 8 is movably fitted with a first piston 20, a bacteria filter 28 is fixedly disposed at a port of the centrifugal tube 8 above the first piston 20, the bacteria filter 28 is a cellulose membrane filter, and the diameter of a filtration pore of the cellulose membrane filter is less than 0.22 μm, which can filter bacteria and prevent bacteria from contaminating blood. Referring to fig. 3, the inner wall of the trim tube 9 is movably fitted with a second piston 21.

Referring to fig. 1, a dual-channel rotary seal 6 is fixedly arranged on a frame 15, a centrifuge cover 13 is fixedly arranged on the frame 15, an upper cover 14 of the centrifuge is hinged to the top of the centrifuge cover 13, a single-channel rotary seal 7 is fixedly arranged on the top of the centrifuge cover 13, and the single-channel rotary seal 7 penetrates through the upper cover 14 of the centrifuge. The port that just is located second piston 21 top on the centrifuging tube 8 has single channel rotary seal 7 through the hose connection, and single channel rotary seal 7 has first three-way pipe 16 through the hose connection, and first three-way pipe 16 passes through hose connection second three-way pipe 22 and third three-way pipe 23, and second three-way pipe 22 has blood bag 24 and plasma bag 25 through the hose connection, and third three-way pipe 23 has reagent bag 26 and nucleated cell bag 27 through the hose connection.

The pipeline between the single-channel rotary seal 7 and the first three-way pipe 16 is a transparent hose, a fluid component sensor 17 is mounted on the transparent hose through binding, the fluid component sensor 17 adopts an American Introtek blood component sensor, non-invasive detection based on optics is carried out on blood components in the transparent hose, and platelet-rich plasma, nucleated cells, reagents, red blood cells and the like can be distinguished. The first three-way pipe 16 is provided with a fluid pressure difference sensor 12, the second three-way pipe 22 and the third three-way pipe 23 are provided with manual valves, and the on-off of the pipelines of the second three-way pipe 22 and the third three-way pipe 23 can be changed through manually operating the manual valves.

The blood component automatic balancing separation method comprises the following steps:

s1, bagging blood samples: filling the blood bag 24 with a blood sample to be separated, and filling the reagent bag 26 with a reagent for assisting in separating the blood sample;

s2, blood sample inlet tube: starting the double-acting air pump 19 to form negative pressure at the upper part of the centrifugal tube 8, so that the blood sample and the reagent enter the upper part of the centrifugal tube 8;

s3, centrifugal balancing: starting the motor 1, and filling or removing liquid with certain mass into or from the balancing pipe 9 by the quality controller 18 according to the vibration signal detected by the vibration sensor 11;

s4, centrifugation: keeping the rotating speed of the motor 1 unchanged until the blood sample in the centrifuge tube 8 is layered, and reducing the rotating speed of the motor 1;

s5, separation and balancing: starting the double-acting air pump 19, pushing the blood sample to be discharged out of the centrifuge tube 8 by the first piston 20, and filling or discharging liquid with certain mass into the balancing tube 9 by the quality controller 18 according to the vibration signal detected by the vibration sensor 11;

s6, separating and bagging: the fluid component sensor 17 generates a blood sample component signal, and the on/off of the second three-way pipe 22 and the third three-way pipe 23 is adjusted according to the signal.

The double-acting air pump 19 is started and operated in a degassing state, and the first piston 20 moves downwards by generating enough pressure difference between the two sides of the first piston 20 in the centrifugal tube 8 through the high-pressure tube. The upper part of the centrifugal tube 8 forms a negative pressure state, and blood samples or reagents in the blood bag 24 and the reagent belt 25 enter the centrifugal tube 8 through the second three-way pipe 22, the third three-way pipe 23 and the single-channel rotary seal 7

The motor 1 drives the rotor 4 to rotate away from the main shaft 3, and vibration is caused by unbalanced mass distribution of the rotor 4 in an initial state. A vibration signal is generated via the vibration sensor 11. After the vibration sensor 11 generates the vibration signal, the quality controller 18 fills or removes a certain mass of liquid into or from the balancing pipe 9 through a high-pressure pipe, and when the rotor 4 tends to be balanced, the vibration signal of the vibration sensor 11 is weakened or disappears, and the quality controller 18 stops.

The rotor 4 with balanced mass distribution centrifuges the blood sample at a required rotation speed, and after centrifugation, the blood sample and the reagent in the centrifuge tube 8 are separated into components with different height positions, such as a platelet-rich plasma layer, a nucleated cell layer, a reagent layer, a red blood cell layer and the like, according to the densities of different components under the action of centrifugal force and other resultant forces.

The motor 1 is decelerated and stopped and the manual valves on the second 22 and third 23 tee are adjusted to the corresponding states according to the known order of the blood components in the centrifuge tube 8. The double-acting air pump 19 is operated to the air-supplementing state, and the first piston 20 moves upwards by generating enough pressure difference on the upper side and the lower side of the first piston 20 in the centrifugal tube 8 through the high-pressure tube. The blood component in the upper 8 parts of the centrifuge tube is discharged outwards. When the blood component flows through the fluid component sensor 17, the fluid component sensor 17 generates a correlation signal corresponding to the blood component, and the operator adjusts the correlation signal and the time according to the correlation signal generated by the fluid component sensor 17 to direct different blood components to corresponding flow directions, such as the plasma bag 25, the nucleated cell bag 26, and the reagent bag 27.

When the motor 1 is decelerated and not stopped, the blood component separation process is consistent with the above process, but the mass distribution balance of the rotor 4 is broken in the process to cause vibration, the vibration sensor 11 generates a vibration signal, the quality controller 18 fills or removes liquid with certain mass into the balancing pipe 9 through a high-pressure pipe after the vibration sensor 11 generates the vibration signal, and when the rotor 4 is balanced, the vibration signal of the vibration sensor 11 is weakened or disappears, and the quality controller 18 stops. The operation of mass balance adjustment of the rotor 4 is performed in the whole blood component discharge process or the whole centrifuge operation process, the balance of the mass distribution of the rotor 4 is constantly maintained, the stability of the centrifugation process is controlled, and the centrifugation effect is ensured.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a blood composition automatic balance separator, includes frame (2), set firmly in motor (1) on frame (2), set firmly in main shaft (3) on the output shaft of motor (1), set firmly in rotor (4) on main shaft (3) are the annular evenly set firmly in well bucket (5) on rotor (4) are inserted and are located in bucket (5) and about main shaft (3) are the centrifuging tube (8) and the balance pipe (9) of symmetric distribution, its characterized in that: the machine base (2) is fixedly provided with a double-channel rotary seal (6), two ends of one channel of the double-channel rotary seal (6) are respectively connected with a lower port of a centrifugal tube (8) and a double-acting air pump (19) through pipelines, two ends of the other channel of the double-channel rotary seal (6) are respectively connected with a lower port of a leveling tube (9) and a quality controller (18) through pipelines, the inner wall of the centrifugal tube (8) is movably matched with a first piston (20), the inner wall of the leveling tube (9) is movably matched with a second piston (21), a port pipeline on the centrifugal tube (8) and above the second piston (21) is connected with a single-channel rotary seal (7), the pipeline of the single-channel rotary seal (7) is connected with a first three-way pipe (16), and the first three-way pipe (16) is connected with a second three-way pipe (22) and a third three-way pipe (23), the second three-way pipe (22) is connected with a blood bag (24) and a plasma bag (25) through pipelines, and the third three-way pipe (23) is connected with a reagent bag (26) and a nucleated cell bag (27) through pipelines;

the bottom of frame (2) has set firmly damping shock absorber (10), the bottom of damping shock absorber (10) has set firmly frame (15), set firmly vibrations sensor (11) on frame (15), the output electric connection of vibrations sensor (11) has the input of quality control ware (18).

2. A blood component automatic balancing and separating apparatus according to claim 1, wherein: a fluid component sensor (17) is arranged on a pipeline between the single-channel rotary seal (7) and the first three-way pipe (16), and the fluid component sensor (17) comprises a blood component sensor.

3. A blood component automatic balancing and separating apparatus according to claim 1, wherein: the centrifuge tube (8) is internally provided with a bacterial filter (28) at a port above the first piston (20), and the bacterial filter (28) comprises a cellulose membrane filter.

4. A blood component automatic balancing and separating apparatus according to claim 1, wherein: the shock sensor (11) comprises an inertial moving coil type electric sensor.

5. A blood component automatic balancing and separating apparatus according to claim 1, wherein: the double-channel rotary seal (6) is fixedly arranged on the frame (15); frame (15) have set firmly centrifuge cover (13), the top of centrifuge cover (13) articulates there is centrifuge upper cover (14), the top of centrifuge cover (13) has set firmly single channel rotary seal (7), single channel rotary seal (7) run through centrifuge upper cover (14).

6. A blood component automatic balancing and separating apparatus according to claim 1, wherein: a fluid pressure difference sensor (12) is arranged on the first three-way pipe (16); electromagnetic valves or manual valves are arranged on the second three-way pipe (22) and the third three-way pipe (23).