CN215308954U - Piston type biological liquid separation cup with inner core - Google Patents

Abstract

The utility model provides a piston type biological liquid separation cup with an inner core, which comprises a liquid inlet, the inner core, a separation cavity and a liquid outlet; the liquid inlet is arranged separately from the liquid outlet; the biological fluid enters the separation chamber through the fluid inlet and the inner core; after the biological liquid is separated in the separation cavity, part of the liquid is discharged through the liquid outlet, and the rest of the liquid is discharged through the inner core and the liquid inlet. The piston type biological liquid separating cup with the inner core can efficiently and continuously treat biological liquid, and the amount of liquid treated once is not limited; the centrifugal path is long, the centrifugal time is sufficient, the centrifugal effect is better, and the cell recovery rate is higher; the inlet and the outlet are arranged separately, so that the liquid inlet, the separation cavity, the flow guide cavity, the liquid outlet and the like can be cleaned, and the loss of target cells is avoided.

Description

Piston type biological liquid separation cup with inner core

Technical Field

The utility model belongs to the field of biological liquid separation, and particularly relates to a piston type biological liquid separation cup with an inner core.

Background

In modern biotechnology, it is often necessary to process a biological fluid to obtain one or several target cells therein. Such as removing redundant plasma and red blood cells in peripheral blood and umbilical cord blood to obtain stem cells or immune cells in the peripheral blood and the umbilical cord blood; or concentrating and washing the cultured cell sap, and removing redundant waste liquid to obtain the cell sap with the target concentration. Since the volume of biological fluid is generally not fixed, for example, the volume of cord blood to be treated is generally 40ml-150ml, and the volume of cell sap to be concentrated and washed is generally 200ml-50L, a currently popular way for treating biological fluid with non-fixed volume is to use a "piston-type" separation cup with variable volume. For example, patent No. CN1331610A discloses a system for separating components of biological fluids, wherein the 'piston type' separating cup matched with the system is described with emphasis. The structure of the separating cup comprises a hollow centrifugal processing chamber which is provided with an axial biological fluid inlet/outlet; the treatment chamber is provided with a movable piston, under the action of an air pump of matched equipment, quantitative biological liquid is sucked into the treatment chamber, and after treatment is finished, the piston moves upwards and extrudes the treated biological liquid component through an outlet; the piston position is monitored by optical means of the associated apparatus, and pressure regulating valve means on the associated apparatus selectively communicate or interrupt communication between the process chamber and the vessel. Patent No. CN109294899A discloses a "preparation method of PBMC cells", which also comprises a "piston centrifuge cylinder" matched with a pneumatic device on the equipment to suck/discharge biological liquid, and the structure of the separation cup is basically the same as that of the separation cup disclosed in patent No. CN 1331610A.

The piston type separating cup can flexibly treat biological liquid with an unfixed volume, but the separating cup in the prior art has the following problems:

(1) the liquid volume of single treatment is small, and biological liquid cannot be continuously treated. Since the single volume of the "piston" separating cup cannot exceed its maximum volume (typically 220ml to 250ml), when the volume of the biological fluid to be treated exceeds its maximum volume, multiple cycles must be performed; when the amount of the treated umbilical cord blood is 40ml to 150ml, the treatment can be directly completed only 1 time if the maximum volume is 250ml (1 cycle comprising suction liquid → centrifugation → discharge waste liquid → collection of target cells); when the amount of the cell fluid to be treated is 2000ml, at least 8 cycles are required, and the efficiency is very low;

(2) the target cells near the inlet are wasted, reducing the target cell recovery. Since the inlet and the outlet of the separating cup are the same, each time the biological fluid to be separated is sucked, some biological fluid is necessarily remained at the inlet. After centrifugation is complete, the "waste" is first removed and the biological fluid containing the target cells at the inlet is also removed, wasting valuable target cells. The greater the number of cycles, the more target cells are wasted.

(3) The more the biological fluid sucked later in each cycle is, the shorter the centrifugal time (with respect to the start of sucking the biological fluid) is, the more the centrifugal is insufficient. Whether the biological liquid is centrifuged sufficiently or not is closely related to the centrifugal time and the centrifugal force; since the centrifugal force G is ω 2r (where ω is the rotational speed and r is the centrifugal radius), the smaller the centrifugal radius of the portion of the liquid closer to the axial center of the top end of the separation cup at the same rotational speed, the smaller the centrifugal force applied thereto, and the less the centrifugal force is sufficient. At the end of centrifugation, a small number of target cells that have not been sufficiently centrifuged are mixed in the waste liquid and discharged together, resulting in low target cell recovery.

SUMMERY OF THE UTILITY MODEL

In order to solve at least one of the technical problems, the utility model adopts the technical scheme that the piston type biological liquid separating cup with the inner core is provided, biological liquid can be continuously treated with high efficiency, and the amount of liquid treated once is not limited; the loss of target cells can be avoided; the centrifugal path is long, the centrifugal time is sufficient, the centrifugal effect is good, and the cell recovery rate is higher.

In order to at least achieve one of the above purposes, the utility model adopts the technical scheme that:

the utility model provides a piston type biological liquid separation cup with an inner core, which comprises a liquid inlet, the inner core, a separation cavity and a liquid outlet; the liquid inlet is arranged separately from the liquid outlet; the biological fluid enters the separation chamber through the fluid inlet and the inner core; after the biological liquid is separated in the separation cavity, part of the liquid is discharged through the liquid outlet, and the rest of the liquid is discharged through the inner core and the liquid inlet.

Furthermore, the inner core is a hollow columnar revolving body, and comprises a hemispherical flow gathering hole, a flow guiding conical surface, an annular baffle, a waist-shaped hole and a strip-shaped hole; the biological liquid sequentially enters the separation cavity through the hemispherical flow gathering hole, the flow guiding conical surface, the annular baffle and the waist-shaped hole, and the hemispherical flow gathering hole, the flow guiding conical surface, the annular baffle and the waist-shaped hole form a flow guiding channel; the strip shaped aperture fluidly communicates the separation chamber with the liquid outlet.

Further, the cup body and the piston are also included; the cup body is a hollow transparent cylinder, the inner core is fixed in the cup body, and the piston is arranged between the inner core and the cup body in a sliding and sealing way and is matched with the inner core and the cup body in a sliding and sealing way; the cup body, the inner core and the piston are coaxially arranged, and the separation cavity is formed by a cavity between the inner surface of the cup body, the drainage conical surface above the piston and the outer surface of the inner core.

Furthermore, a first flange and a second flange are arranged on the outer side surface of the bottom of the cup body, and a step end surface is arranged on the end surface of the bottom; the piston is characterized by further comprising a base, wherein the base is disc-shaped, an air inlet hole is formed in the bottom of the base, a limiting ring, a limiting groove and a third flange are arranged on the upper portion of the base, a plurality of small holes for ventilation are uniformly distributed in the limiting ring, and the air inlet hole is communicated with a pneumatic cavity below the piston through fluid in the small holes; the outer side of the base is provided with a hollow annular bottom cover, the edge of the bottom cover is provided with a plurality of uniformly distributed buckles, and the buckles are matched with the second flange to fix the base at the bottom of the cup body.

The fixing head is a hollow multi-step columnar body and comprises a first step shaft, a second step shaft and a circular disc; the liquid inlet is arranged on the first stepped shaft in a penetrating mode, and the liquid outlet is arranged on the second stepped shaft in a penetrating mode; the bottom of circular disk is provided with first bulge loop, second bulge loop and third bulge loop outer flange, first slot and second slot have set gradually from outside to inside between first bulge loop, second bulge loop and the third bulge loop.

Further, the novel anti-falling device comprises a first lining and a second lining, wherein the first lining and the second lining are in inverted funnel-like shapes; the first inner liner comprises a first small-end hollow cylinder and a first large-end conical body, and a step shaft and an annular groove are arranged on the outer side of the first small-end hollow cylinder; a plurality of first supporting strips which are uniformly distributed are arranged on the inner surface of the first small-end hollow cylinder along the axial direction; the second liner comprises a second small-end hollow cylinder and a second large-end conical body; a hollow stepped hole is formed in the second small-end hollow cylinder; a plurality of second supporting strips which are uniformly distributed are arranged on the outer side surface of the second large-end conical body; a second movable sealing ring is arranged on the annular groove of the first liner; the stepped shaft of the first small-end hollow cylinder on the first inner liner is inserted into the second stepped shaft of the fixing head in a shape matching manner and is abutted and fixed with the third convex ring; after the second small-end hollow cylinder of the second lining penetrates through the first small-end hollow cylinder, the stepped shaft at the end part is bonded on the stepped hole of the fixing head, the outer surface of the second small-end hollow cylinder is abutted with the first supporting strip inside the first lining hollow cylinder, and meanwhile, the second supporting strip of the second lining is abutted with the inner side of the first large-end conical body of the first lining; the honeycomb duct is bonded in the hollow stepped hole; the draft tube, the inner surface of the first small-end hollow cylinder and the liquid inlet form a liquid inlet channel; the liquid outlet channel is formed by the strip-shaped hole, the outer side surface of the second big-end conical body, the inner surface of the first small-end hollow cylinder, the outer side of the second small-end hollow cylinder, the inner side of the first small-end hollow cylinder and the liquid outlet.

The bearing seat is a cylindrical hollow revolving body, the upper part of the bearing seat comprises an upper convex ring and a third groove, the middle of the bearing seat is provided with a through hole, and an upper step ring surface is arranged between the upper convex ring and the through hole; the lower part of the bearing seat is provided with an annular step surface; a first static sealing ring is arranged in the third groove, and a first dynamic sealing ring is arranged on the ring surface of the upper step; the upper convex ring is bonded on the first groove, and the first convex ring is bonded in the third groove; the first convex ring and the second convex ring are respectively abutted with the first static sealing ring and the first dynamic sealing ring.

The cup cover comprises a third small-end hollow cylinder and a third large-end conical body, and a fourth convex ring and a fourth groove are arranged on the bottom end face of the third large-end conical body; a fifth convex ring and a fifth groove are arranged at the upper end of the cup body; the fourth bulge loop is bonded in the fifth groove, and the fifth bulge loop is bonded in the fourth groove.

The bearing is installed in a bearing hole of the bearing seat in an interference fit mode through an outer ring; a third small-end hollow cylinder of the cup cover is in interference fit with and positioned in the inner ring of the bearing and is inserted into the second groove; the outer surface of the third small-end hollow cylinder is in interference fit with the first dynamic sealing ring, and the third small-end hollow cylinder and the first dynamic sealing ring are in rotary sealing; and the inner surface of the third small-end hollow cylinder is in interference fit with the second dynamic sealing ring, and the third small-end hollow cylinder and the second dynamic sealing ring are in rotary sealing.

Compared with the prior art, the piston type biological liquid separating cup with the inner core has the beneficial effects that:

(1) compared with the common piston type separating cup, the inner core is added, and the fluid channel from the inlet to the outlet is established in the separating cup, so that the biological liquid can be efficiently and continuously treated, and the amount of liquid treated once is not limited; can separate biological liquid such as small-volume umbilical cord blood, peripheral blood, bone marrow and the like, and can also concentrate and wash large-volume cell sap and the like;

(2) by adding the inner core, the minimum centrifugal force of the biological liquid in the separation cavity is limited, a better centrifugal effect is obtained, and the recovery rate of the target cells is higher;

(3) establishing a fluid channel through which the biological liquid flows from the inlet of the separation cup, the flow guide pipe in the center and the flow guide cavity to the bottom of the separation cavity and then upwards diffuses to the liquid outlet; from the inlet to the outlet, the centrifugal path is long, the centrifugal time is sufficient, the centrifugal effect is better, and the cell recovery rate is higher;

(4) the inlet and the outlet of the separation cup are separately arranged, so that the liquid inlet, the separation cavity, the flow guide cavity, the liquid outlet and the like can be cleaned, the loss of target cells is avoided, and the recovery rate of the cells is also improved.

Drawings

FIG. 1 is a schematic structural view of a piston-type biological fluid separation cup with an inner core according to the present invention;

FIG. 2 is an exploded view of the piston-type biological fluid separation cup with an inner core of the present invention;

FIG. 3 is a schematic structural view of a retaining head of the present invention;

FIG. 4 is a schematic view of a first liner of the present invention;

FIG. 5 is a cross-sectional view taken along line B-B of FIG. 4;

FIG. 6 is a schematic diagram of a second liner of the present invention;

FIG. 7 is a view in the direction M of FIG. 6;

FIG. 8 is a schematic view of a bearing housing of the present invention;

FIG. 9 is a schematic structural view of the lid of the present invention;

FIG. 10 is a schematic view of the construction of the cup of the present invention;

FIG. 11 is a schematic view of the piston of the present invention;

FIG. 12 is a schematic diagram of the structure of the kernel of the present invention;

FIG. 13 is a schematic structural view of a base according to the present invention;

FIG. 14 is a schematic view of the internal structure of the base of the present invention;

FIGS. 15-19 are schematic views of the process of the separation cup of the present invention when the volume of separated biological fluid can be processed in a single pass;

FIGS. 20-23 are schematic views of the separation cup of the present invention during continuous processing of separated volumes of biological fluid.

Wherein, 1 fixed head, 1A first step shaft, 1A1 step hole, 1B second step shaft, 1C round disc, 1C1 first convex ring, 1C2 second convex ring, 1C3 third convex ring, 1C4 outer flange, 1C5 first groove, 1C6 second groove, 1D liquid inlet, liquid outlet 1E, 2 first static sealing ring, 3 first dynamic sealing ring, 4 bearing seat, 4A upper convex ring, 4B third groove, 4C through hole, 4D upper step ring surface, 4E annular step surface, 5 bearing, 6 cup cover, 6A third small end hollow cylinder, 6B third large end cone, 6B1 fourth convex ring, 6B2 fourth groove, 7 second dynamic sealing ring, 8 first inner lining, 8A first small end hollow cylinder, 8A1 step shaft, 8A2 annular groove, 8B first large end cone, 8C 9 second supporting bar, 9 small bar hollow cylinder end bar, 9A small bar hollow cylinder end bar, 9A1 hollow step hole, 9B second big end cone, 9B1 second support bar, 10 honeycomb duct, 11 inner core, 11A first step column, 11B second step column, 11C third step column, 11D step end face, 11E strip hole, 11F waist hole, 11G circular ring baffle, 11H guide cone, 11J semispherical convergence hole, 12 cup body, 12A fifth bulge loop, 12B fifth groove, 12C first flange, 12D second flange, 12E stepped end face, 13 third piston sealing ring, 14 first piston sealing ring, 15 piston, 15A sixth groove, 15B seventh groove, 15C eighth groove, 15D ninth groove, 15E drainage conical surface, 15F air pressure cavity, 16 second piston sealing ring, 17 second static sealing ring, 18 base, 18A air inlet hole, 18B spacing ring, 18C spacing groove, 18D third flange, 18E small hole and 19 bottom cover.

Detailed Description

In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to specific examples. Note that the following described embodiments are illustrative only for explaining the present invention, and are not to be construed as limiting the present invention. The examples, where specific techniques or conditions are not indicated, are to be construed according to the techniques or conditions described in the literature in the art or according to the product specifications.

In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; the connection can be mechanical connection or electrical connection; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

The piston type biological liquid separating cup with an inner core provided by the utility model is described in detail by the following specific embodiments:

as shown in the attached drawings 1 to 14, the piston type biological liquid separation cup with the inner core mainly comprises a fixed head 1, a first static sealing ring 2, a first dynamic sealing ring 3, a bearing seat 4, a bearing 5, a cup cover 6, a second dynamic sealing ring 7, a first inner liner 8, a second inner liner 9, a flow guide pipe 10, an inner core 11, a cup body 12, a third piston sealing ring 13, a first piston sealing ring 14, a piston 15, a second piston sealing ring 16, a second static sealing ring 17, a base 18, a bottom cover 19 and other parts.

As shown in fig. 3, the main body of the fixed head 1 is a hollow multi-step columnar body, and includes a first stepped shaft 1A, a second stepped shaft 1B, and a circular disk 1C. A hollow columnar liquid inlet 1D is arranged on the first stepped shaft 1A in a penetrating manner, and a stepped hole 1A1 is also arranged in the first stepped shaft 1A; a hollow columnar liquid outlet 1E is arranged on the second stepped shaft 1B in a penetrating way; the bottom of the circular disc 1C is provided with a first convex ring 1C1, a second convex ring 1C2 and a third convex ring 1C3, and an outer flange 1C4, a first groove 1C5 and a second groove 1C6 are sequentially arranged between the convex rings from outside to inside.

As shown in fig. 4-5, the first liner 8 is shaped like an inverted funnel and includes a first small end hollow cylinder 8A and a first large end cone 8B. A step shaft 8A1 and an annular groove 8A2 are arranged outside the first small-end hollow cylinder 8A; the inner surface of the first small-end hollow cylinder 8A is provided with three first supporting strips 8C which are uniformly distributed along the axial direction.

As shown in fig. 6-7, the second liner 9 is also shaped like an inverted funnel, comprising a second small end hollow cylinder 9A and a second large end cone 9B. A hollow step hole 9A1 is formed in the second small-end hollow cylinder 9A; three second supporting strips 9B1 which are uniformly distributed are arranged on the outer side surface of the second big-end conical body 9B.

As shown in fig. 8, the bearing seat 4 is a cylindrical hollow solid of revolution, the upper portion of the bearing seat 4 includes an upper convex ring 4A and a third groove 4B, a through hole 4C is provided in the middle, and an upper step ring surface 4D is provided between the upper convex ring 4A and the through hole 4C; the lower portion of the bearing housing 4 is provided with an annular step face 4E.

The annular groove 8A2 of the first liner 8 is provided with a second dynamic sealing ring 7; the stepped shaft 8A1 of the first small-end hollow cylinder 8A of the first liner 8 is inserted into the second stepped shaft 1B of the fixed head 1 in a form-fitting manner and is fixed in abutment with the third collar 1C 3. After the second small end hollow cylinder 9A of the second liner 9 passes through the first small end hollow cylinder 8A, the stepped shaft at the end part is bonded on the stepped hole 1a1 of the fixed head 1, the outer surface of the second small end hollow cylinder 9A is abutted with the three first supporting strips 8C inside the first liner hollow cylinder 8A, and simultaneously, the three second supporting strips 9B1 of the second liner 9 are abutted with the inner side of the first large end conical body 8B of the first liner 8. The draft tube 10 is a hollow long round tube, and the outer wall and the upper end face of the draft tube 10 are bonded on the hollow step hole 9A1 in the second liner 9. The bearing 5 is arranged in a bearing hole of the bearing seat 4 through the interference fit of a bearing outer ring; the first static sealing ring 2 is arranged in a third groove 4B of the bearing seat 4, and the first dynamic sealing ring 3 is arranged on an upper step ring surface 4D of the bearing seat 4. The upper collar 4A of the bearing housing 4 is bonded to the first groove 1C5 of the fixed head 1, and the first collar 1C1 of the fixed head 1 is bonded to the third groove 4B of the bearing housing 4. The first convex ring 1C1 and the second convex ring 1C2 of the fixed head 1 are respectively in contact with the first static seal ring 2 and the first dynamic seal ring 3 which are installed on the bearing seat 4 in a squeezing manner, so that the space between the fixed head 1 and the bearing seat 4 is sealed. To sum up, the fixed head 1, the first lining 8, the second lining 9, the flow guide pipe 10 and the bearing seat 4 are fixedly connected together to form a static component of the separation cup, namely, the static component is in a static state when the separation cup is separated.

As shown in fig. 9, the cup cover 6 is an inverted funnel-like revolving body, and includes a third small end hollow cylinder 6A and a third large end conical body 6B, and a fourth convex ring 6B1 and a fourth groove 6B2 are provided on a bottom end surface of the third large end conical body 6B.

As shown in fig. 10, cup 12 is a hollow transparent cylinder. The upper end of the cup body 12 is provided with a fifth convex ring 12A and a fifth groove 12B which are matched with the cup cover, and the inner side of the upper end is provided with a stepped cylindrical surface. The outer side surface of the bottom of the cup body 12 is provided with a first flange 12C and a second flange 12D, and the end surface of the bottom is provided with a step end surface 12E.

As shown in fig. 11, the piston 15 is a hollow columnar rotator. A sixth groove 15A and a seventh groove 15B for installing sealing rings are arranged on the outer side of the piston 15; an eighth groove 15C and a ninth groove 15D for installing sealing rings are arranged on the inner side of the piston 15; a drainage conical surface 15E is arranged above the piston 15; the bottom of the piston 15 is provided with a pneumatic chamber 15F.

As shown in fig. 12, the inner core 11 is a stepped cylindrical shell. The inner core 11 comprises a first step pillar 11A, a second step pillar 11B and a third step pillar 11C; there is step terminal surface 11D between first step post 11A and the second step post 11B, and second step post 11B is provided with four bar holes 11E of evenly distributed with third step post 11C's junction, is provided with the waist shape hole 11F of equipartition on the third step post 11C, and third step post 11C's inboard is provided with ring baffle 11G, water conservancy diversion conical surface 11H and hemisphere and gathers discharge orifice 11J.

As shown in fig. 13-14, the base 18 is disc-shaped. The bottom of base 18 is provided with inlet port 18A, and the upper portion of base 18 is provided with spacing ring 18B, spacing groove 18C and third flange 18D, and the equipartition has four apertures 18E that are used for ventilating on the spacing ring 18B.

The first stepped column 11A, the second stepped column 11B and the step end face 11D of the core 11 are bonded to the stepped cylindrical surface of the cup body 12 and positioned. A first piston sealing ring 14 and a second piston sealing ring 16 are respectively arranged in a sixth groove 15A and a seventh groove 15B of the piston 15; the eighth groove 15C and the ninth groove 15D of the piston 15 are respectively provided with a third seal ring 13 therein. Piston 15 is slidably disposed on inner core 11 and is slidable between inner core 11 and cup 12, and first and second piston seals 14 and 16 contact and seal with the inner surface of cup 12, and third seal 13 contacts and seals with the outer surface of inner core 11. The base 18 is arranged at the bottom of the cup body 12, wherein a second static sealing ring 17 is arranged on a step end surface 12E at the bottom of the cup body 12, and a third flange 18D of the base 18 is contacted with the end surface of the cup body 12 and presses the second static sealing ring 17; the bottom end face of the core 11 is inserted into the stopper groove 18C of the base 18. A hollow annular bottom cover 19 is arranged on the outer side of the base 18, a plurality of evenly distributed buckles are arranged on the edge of the bottom cover 19, and the buckles are matched with the second flange 12D of the cup body 12 to fix the base 18 on the bottom of the cup body 12. Lid 6 is bonded to the upper portion of cup 12 with fourth collar 6B1 of lid 6 bonded in fifth groove 12B of cup 12 and fifth collar 12A of cup 12 bonded in fourth groove 6B2 of lid 6. In summary, the cup cover 6, the inner core 11, the cup body 12, the piston 15, the base 18 and the bottom cover 19 form a rotating assembly of the separating cup, namely, the separating cup is in a high-speed rotating state when being separated.

A third small-end hollow cylinder 6A of a cup cover 6 in the rotating assembly is in interference fit with an inner ring of a bearing 5 and is positioned, and the third small-end hollow cylinder is inserted into a second groove 1C6 of the fixed head 1; the outer surface of the third small-end hollow cylinder 6A is in interference fit with the first dynamic seal ring 3, and the third small-end hollow cylinder and the first dynamic seal ring can be rotationally sealed; the inner surface of the third small-end hollow cylinder 6A is in interference fit with the second dynamic seal ring 7, and the third small-end hollow cylinder and the second dynamic seal ring can be rotationally sealed; thereby realize carrying out rotation support sealing connection through the bearing between rotatory subassembly and the stationary assembly.

In the separating cup provided by the utility model, a liquid inlet channel is formed by the honeycomb duct 10, the inner surface of the first small end hollow cylinder 8A of the second lining 8 and the liquid inlet 1D at the top of the fixing head 1; the hemispherical convergence hole 11J, the flow guide conical surface 11H, the annular baffle 11G and the waist-shaped hole 11F of the inner core 11 form a flow guide channel; the inner surface of the cup body 12, the drainage conical surface 15E and the outer surface of the inner core 11 enclose a separation cavity, and when the piston 15 is positioned at the bottommost part, the dynamic volume of the separation cup is the largest, and is preferably 250 ml; the separation chamber becomes progressively smaller as the piston 15 moves upwards, and the dynamic volume of the separation cup is at a minimum, preferably 30ml, as the piston 15 moves to the top; a liquid outlet channel is formed among the strip-shaped hole 11E above the inner core 11, the outer side surface of the second big-end conical body 9B, the inner surface of the first small-end hollow cylinder 8A, the outer side of the second small-end hollow cylinder 9A, the inner side of the first small-end hollow cylinder 8A and the liquid outlet 1E of the fixed head 1; an air inlet hole 18A of the base 18, a small hole 18E on the limiting ring 18B and an air pressure cavity 15F at the bottom of the piston 15 form an air inlet channel and an air outlet channel, and the air inlet channel and the air outlet channel are not communicated with the separation cavity at any time. In conclusion, when the separation cup provided by the utility model is used for separation, biological liquid sequentially passes through the liquid inlet channel, the flow guide channel, the separation cavity and the liquid outlet channel for separation, so that a fluid channel from the liquid inlet to the liquid outlet is established in the separation cup, the centrifugal path is long, the centrifugal time is sufficient, and the centrifugal effect is better; the inlet and the outlet are separately arranged, so that a liquid inlet, a separation cavity, a flow guide cavity, a liquid outlet and the like can be cleaned, and the loss of target cells is avoided; meanwhile, the piston is actuated through the air inlet channel and the air outlet channel, so that the volume of the separation cavity can be changed, the biological liquid can be efficiently and continuously treated, and the amount of the liquid treated once is not limited.

The first static sealing ring 2 and the second static sealing ring 17 provided by the utility model are preferably common rubber rings, preferably NBR materials; the first dynamic sealing ring 3 and the second dynamic sealing ring 7 are preferably fluororubber rings with smooth surfaces; the first piston seal ring 14, the second piston seal ring 16 and the third piston seal ring 13 are preferably silicone rings with smooth surfaces.

The separation method of the piston type biological liquid separation cup with the inner core provided by the utility model is specifically introduced as follows:

1. as shown in the attached figures 15-19, when the volume of the biological fluid to be separated is 40-250ml, taking the separation of 120ml cord blood +30ml gradient fluid as an example, the method comprises the following steps:

a, a centrifuge on the separation equipment drives a rotating component of a separation cup to rotate at a high speed;

b, taking a peristaltic pump or other power sources as power, and conveying a certain amount of gradient liquid ficoll to a hemispherical flow gathering hole at the bottom of the inner core through a liquid inlet channel; under the action of centrifugal force, the gradient liquid quickly climbs to the position of the circular baffle plate along the diversion channel and then quickly climbs to the separation cavity along the kidney-shaped hole and the drainage conical surface;

c, conveying the umbilical cord blood to be separated to a hemispherical flow gathering hole at the bottom of the inner core from the liquid inlet channel by using a peristaltic pump; similarly, the umbilical cord blood can rapidly enter the separation cavity along the diversion channel; when the cord blood completely enters the separation cup, all cord blood will be in the separation chamber. After a period of centrifugation, the heavier red blood cells are positioned at the outermost side of the separation cavity and then are sequentially provided with gradient liquid, a leucocyte layer and plasma inwards;

d, using an air pump to push the piston through the air inlet and outlet channels, so that the volume of the separation cavity is gradually reduced, and the plasma and the tunica albuginea layer on the inner side are pushed out in sequence; the required components are collected by matching with a valve, a color sensor, a pipeline component sensor, a component collecting bag and a waste liquid bag on the separation equipment;

e, when only the red blood cells and the gradient liquid are left in the separation cavity, reversely exhausting air by the air pump, actuating the piston to move downwards, and slowly stopping the centrifuge; the liquid in the separation cavity flows into the hemispherical flow gathering hole under the action of gravity; and under the action of the peristaltic pump, the residual liquid is sucked away from the guide pipe, and the separation is completed.

2. As shown in FIGS. 20-23, when the volume of the biological fluid to be separated is larger than 250ml, the cell concentration of 1000ml is counted as 1X 10⁷The cell sap of each ml was concentrated to a volume of 100ml with a cell count of 1X 10⁸The method comprises the following steps of:

a, a centrifugal machine on the separation equipment drives a rotating assembly of a separation cup to rotate at a high speed;

the device B takes a peristaltic pump or other power sources as power to convey cell liquid from a liquid inlet channel to the hemispherical flow gathering hole at the bottom of the inner core; under the action of centrifugal force, cell sap quickly climbs to the position of the circular baffle along the diversion channel, then quickly climbs to the separation cavity along the kidney-shaped hole and the drainage conical surface, and the separation cavity is gradually filled with the cell sap; in the process that the cell sap fills the separation cavity, cells in the cell sap are distributed on the outermost side of the separation cavity under the action of centrifugal force, and the waste liquid is positioned on the innermost side of the separation cavity;

c, along with the continuous input of the cell sap, extruding the waste liquid without cells at the innermost side in the separation cavity; when the cell sap is completely input into the separation cup, the cell is arranged at the outer side of the separation cavity, the waste liquid is arranged at the inner side of the separation cavity, and the total volume is 250ml of the maximum dynamic volume of the separation cup;

d, starting an air pump to push the piston, matching with components such as a gravity sensor, an optical sensor and a valve on the separation equipment, and stopping pushing the piston until the residual volume in the separation cup is 100 ml;

stopping the centrifuge, starting the air pump, and reversely pumping air to move the piston to the bottommost part; under the action of gravity, all cell sap in the separation cup is collected to the hemispherical flow collecting hole at the bottom of the inner core; then starting a peristaltic pump to suck the residual cell sap away from the flow guide pipe;

f, sucking a small amount of cleaning liquid from the liquid outlet, starting the centrifugal machine, and cleaning the liquid outlet, the liquid outlet channel, the separation cavity and the flow guide channel; then starting the peristaltic pump again to suck the residual cells into the product bag from the liquid inlet channel; completing the continuous concentration of the biological fluid.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Claims (9)

1. A piston type biological liquid separation cup with an inner core is characterized in that the separation cup comprises a liquid inlet, the inner core, a separation cavity and a liquid outlet; the liquid inlet is arranged separately from the liquid outlet; the biological fluid enters the separation chamber through the fluid inlet and the inner core; after the biological liquid is separated in the separation cavity, part of the liquid is discharged through the liquid outlet, and the rest of the liquid is discharged through the inner core and the liquid inlet.

2. The piston-type biological liquid separation cup with the inner core according to claim 1, wherein the inner core is a hollow columnar rotator and comprises a hemispherical flow gathering hole, a flow guiding conical surface, a circular baffle, a waist-shaped hole and a strip-shaped hole; the biological liquid sequentially enters the separation cavity through the hemispherical flow gathering hole, the flow guiding conical surface, the annular baffle and the waist-shaped hole, and the hemispherical flow gathering hole, the flow guiding conical surface, the annular baffle and the waist-shaped hole form a flow guiding channel; the strip shaped aperture fluidly communicates the separation chamber with the liquid outlet.

3. The piston-type biological fluid separator cup with an inner core of claim 2 further comprising a cup body and a piston; the cup body is a hollow transparent cylinder, the inner core is fixed in the cup body, and the piston is arranged between the inner core and the cup body in a sliding and sealing way and is matched with the inner core and the cup body in a sliding and sealing way; the cup body, the inner core and the piston are coaxially arranged, and the separation cavity is formed by a cavity between the inner surface of the cup body, the drainage conical surface above the piston and the outer surface of the inner core.

4. The piston-type biological liquid separation cup with the inner core according to claim 3, wherein the outer side surface of the bottom of the cup body is provided with a first flange and a second flange, and the end surface of the bottom is provided with a step end surface; the piston is characterized by further comprising a base, wherein the base is disc-shaped, an air inlet hole is formed in the bottom of the base, a limiting ring, a limiting groove and a third flange are arranged on the upper portion of the base, a plurality of small holes for ventilation are uniformly distributed in the limiting ring, and the air inlet hole is communicated with a pneumatic cavity below the piston through fluid in the small holes; the outer side of the base is provided with a hollow annular bottom cover, the edge of the bottom cover is provided with a plurality of uniformly distributed buckles, and the buckles are matched with the second flange to fix the base at the bottom of the cup body.

5. The piston-type biological fluid separation cup with an inner core according to claim 3, further comprising a fixed head, wherein the fixed head is a hollow multi-step cylindrical body, and the fixed head comprises a first step shaft, a second step shaft and a circular disk; the liquid inlet is arranged on the first stepped shaft in a penetrating mode, and the liquid outlet is arranged on the second stepped shaft in a penetrating mode; the bottom of circular disk is provided with first bulge loop, second bulge loop and third bulge loop outer flange, first slot and second slot have set gradually from outside to inside between first bulge loop, second bulge loop and the third bulge loop.

6. The piston-type biological liquid separation cup with a core of claim 5 further comprising a first liner and a second liner, wherein the first liner and the second liner are each in the shape of an inverted funnel; the first inner liner comprises a first small-end hollow cylinder and a first large-end conical body, and a step shaft and an annular groove are arranged on the outer side of the first small-end hollow cylinder; a plurality of first supporting strips which are uniformly distributed are arranged on the inner surface of the first small-end hollow cylinder along the axial direction; the second liner comprises a second small-end hollow cylinder and a second large-end conical body; a hollow stepped hole is formed in the second small-end hollow cylinder; a plurality of second supporting strips which are uniformly distributed are arranged on the outer side surface of the second large-end conical body; a second movable sealing ring is arranged on the annular groove of the first liner; the stepped shaft of the first small-end hollow cylinder on the first inner liner is inserted into the second stepped shaft of the fixing head in a shape matching manner and is abutted and fixed with the third convex ring; after the second small-end hollow cylinder of the second lining penetrates through the first small-end hollow cylinder, the stepped shaft at the end part is bonded on the stepped hole of the fixing head, the outer surface of the second small-end hollow cylinder is abutted with the first supporting strip inside the first lining hollow cylinder, and meanwhile, the second supporting strip of the second lining is abutted with the inner side of the first large-end conical body of the first lining; the honeycomb duct is bonded in the hollow stepped hole; the draft tube, the inner surface of the first small-end hollow cylinder and the liquid inlet form a liquid inlet channel; the liquid outlet channel is formed by the strip-shaped hole, the outer side surface of the second big-end conical body, the inner surface of the first small-end hollow cylinder, the outer side of the second small-end hollow cylinder, the inner side of the first small-end hollow cylinder and the liquid outlet.

7. The piston-type biological liquid separation cup with the inner core according to claim 6, further comprising a bearing seat, wherein the bearing seat is a cylindrical hollow revolving body, the upper part of the bearing seat comprises an upper convex ring and a third groove, the middle part of the bearing seat is provided with a through hole, and an upper step ring surface is arranged between the upper convex ring and the through hole; the lower part of the bearing seat is provided with an annular step surface; a first static sealing ring is arranged in the third groove, and a first dynamic sealing ring is arranged on the ring surface of the upper step; the upper convex ring is bonded on the first groove, and the first convex ring is bonded in the third groove; the first convex ring and the second convex ring are respectively abutted with the first static sealing ring and the first dynamic sealing ring.

8. The piston-type biological liquid separation cup with the inner core according to claim 7, further comprising a cup cover, wherein the cup cover comprises a third small-end hollow cylinder and a third large-end conical body, and a fourth convex ring and a fourth groove are arranged on the bottom end face of the third large-end conical body; a fifth convex ring and a fifth groove are arranged at the upper end of the cup body; the fourth bulge loop is bonded in the fifth groove, and the fifth bulge loop is bonded in the fourth groove.

9. The piston-type biological fluid separation cup with an inner core of claim 8 further comprising a bearing mounted in a bearing bore of the bearing housing by an outer race interference fit; a third small-end hollow cylinder of the cup cover is in interference fit with and positioned in the inner ring of the bearing and is inserted into the second groove; the outer surface of the third small-end hollow cylinder is in interference fit with the first dynamic sealing ring, and the third small-end hollow cylinder and the first dynamic sealing ring are in rotary sealing; and the inner surface of the third small-end hollow cylinder is in interference fit with the second dynamic sealing ring, and the third small-end hollow cylinder and the second dynamic sealing ring are in rotary sealing.

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