CN115340933A - Biological sample separation and extraction device - Google Patents

Abstract

The invention relates to the technical field of biological sample separation, in particular to a biological sample separation and extraction device which comprises a sampling box, a homogenizer, a balance liquid container, a pump assembly, a pipeline system, a centrifugal assembly and an anaerobic system for creating an anaerobic environment for the separation and extraction device, wherein the sampling box is arranged in the homogenizer, the centrifugal assembly comprises a centrifugal disc communicated with the pipeline system, the sampling box, the balance liquid container and the centrifugal disc are communicated with each other through the pipeline system, and the pump assembly is connected with the pipeline system to provide power for pumping liquid in the pipeline system. The centrifugal disc type liquid sampler pumps liquid through the pump assembly, and separates and extracts a biological sample through the centrifugal disc, so that the centrifugal disc type liquid sampler is sanitary and environment-friendly, and can quickly and efficiently extract the biological sample; in addition, the invention separates and extracts the biological sample in the anaerobic environment, which can effectively avoid the adverse effect of oxygen on the quality of the biological sample.

Description

Biological sample separation and extraction device

Technical Field

The invention relates to the technical field of biological sample separation, in particular to a biological sample separation and extraction device.

Background

In human intestinal tracts, a large number of flora maintain normal running of the intestinal tracts, some pathological changes and intestinal digestion dysfunction are caused by some undesirable flora, and intestinal bacteria are extracted from excrement to judge whether the flora in the intestinal tracts is balanced or not clinically. At present, the conventional examination of feces is to esterify feces bacteria, dilute the feces bacteria according to a certain proportion, and then culture and calculate to obtain the total number of various cell flora. However, this method is complicated to operate, long in detection time, unhygienic, not environment-friendly, and harsh in operation environment, and is not favorable for the product quality of the intestinal flora solution.

Chinese patent 201520959785.5 discloses an extraction system for obtaining intestinal microbial flora from human excrement, which comprises a sampling bag, a homogenizing and filtering device and a centrifuge; the homogenizing filtering device comprises a homogenizing filter bag and a homogenizer; and a filter membrane interlayer is arranged in the homogeneous filter bag. Although the scheme is sanitary and environment-friendly and can quickly and efficiently extract intestinal flora, when the scheme is used for separating biological samples, the samples are easily exposed in the air in the processes of transferring the samples and adding diluent, so that the product quality of the biological samples cannot be guaranteed.

Disclosure of Invention

The invention aims to overcome the defects in the prior art, and provides a biological sample separation and extraction device which is sanitary and environment-friendly, can quickly and efficiently extract a biological sample, and can improve and ensure the product quality of the biological sample.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

the biological sample separation and extraction device comprises a sampling box, a homogenizer, a balance liquid container, a pump assembly, a pipeline system, a centrifugal disc and an anaerobic system for creating an anaerobic environment for the separation and extraction device, wherein the sampling box is arranged in the homogenizer, the sampling box, the balance liquid container and the centrifugal disc are communicated with each other through the pipeline system, and the pump assembly is connected to the pipeline system to provide power for pumping liquid in the pipeline system; the sampling box comprises a box body, a cover body and a filtering basket, the box body is detachably connected with the cover body, the cover body is connected with the filtering basket, and the filtering basket is located in the box body.

According to the biological sample separation and extraction device, before separation, an anaerobic environment is formed in the separation and extraction device by adopting an anaerobic system, and the whole pipeline system is closed; diluting a biological sample in a balance liquid, loading the biological sample in a sampling box, crushing and homogenizing the biological sample by a homogenizer, filtering the biological sample, pumping the obtained product to a centrifugal disc under the action of a pump assembly, and centrifuging the obtained product by the centrifugal disc to separate impurities from the biological sample; and pumping the balance liquid to a centrifugal disc for washing and filtering under the action of a pump assembly so as to improve the separation quality of the biological sample. The centrifugal disc type liquid sampler pumps liquid through the pump assembly, and separates and extracts a biological sample through the centrifugal disc, so that the centrifugal disc type liquid sampler is sanitary and environment-friendly, and can quickly and efficiently extract the biological sample; in addition, the invention can effectively avoid the adverse effect of oxygen on the quality of the biological sample by extracting the biological sample in an anaerobic environment.

Furthermore, the filtering basket is arranged close to the inner wall of one side of the box body, the filtering basket is provided with a top surface and a first side surface, a second side surface, a third side surface and a fourth side surface which are connected with the top surface respectively, the first side surface, the second side surface, the third side surface and the fourth side surface are sequentially connected end to end, the first side surface is arranged close to the inner wall of the box body, filtering holes are formed in the top surface, the first side surface, the second side surface and the fourth side surface, and filtering holes are not formed in the third side surface.

Further, the inner wall of the box body is provided with a plurality of first ribs, and the inner wall of the cover body is connected with a vertical plate and a plurality of second ribs.

Furthermore, the cover body is provided with an installation block, the installation block is connected with the filter basket in a sealing mode, and the installation block is provided with an installation step used for installing the first filter membrane.

Furthermore, the anaerobic system comprises an inert gas bottle, an air pump and a valve, the air pump is communicated between the inert gas bottle and the pipeline system, and the valve is arranged between the air pump and the pipeline system.

Further, the pump assembly includes first peristaltic pump and second peristaltic pump, tubing includes connecting in the first pipe return circuit between sample box and centrifugal disc and connecting in the second pipe return circuit between balanced liquid container and centrifugal disc, first peristaltic pump, second peristaltic pump are located first pipe return circuit, second pipe return circuit respectively.

Furthermore, the pipeline system comprises a first pipeline and a second pipeline which are communicated, the sampling box and the balance liquid container are both communicated with the first pipeline, the centrifugal disc is communicated with the end part of the second pipeline, and a rotary joint is arranged at the joint of the first pipeline and the second pipeline.

Further, the centrifugal assembly comprises a motor and a rotating arm, the rotating arm is connected to the output end of the motor, and the centrifugal disc is connected to one end, far away from the motor, of the rotating arm.

Further, the centrifugal disc comprises a first shell, a second shell and a second filter membrane connected between the first shell and the second shell, a collecting cavity is formed between the first shell and the second filter membrane, a waste liquid cavity is formed between the second shell and the second filter membrane, the collecting cavity is communicated with a liquid inlet communicated with a pipeline system, the waste liquid cavity is communicated with a liquid outlet, and the aperture of the second filter membrane is smaller than the diameter of a biological sample.

Further, the collection cavity is of an inverted cone structure, and the cross-sectional area of the inverted cone structure is gradually reduced towards the direction away from the second filter membrane.

Furthermore, the aperture of the second filter membrane is 0.1-0.45 μm, the centrifugal speed of the centrifugal module is 7000-15000 rpm, and the centrifugal acceleration is 3000-30000 g.

Compared with the prior art, the invention has the beneficial effects that:

the biological sample separation and extraction device is sanitary and environment-friendly, can quickly and efficiently extract a biological sample, and can effectively avoid adverse effects of oxygen on the quality of the biological sample.

Drawings

FIG. 1 is a system diagram of a biological sample separation and extraction device;

FIG. 2 is a schematic structural diagram of a centrifugal disc of the biological sample separation and extraction device;

FIG. 3 is a schematic system diagram of another biological sample separation and extraction device;

FIG. 4 is a schematic view of a cartridge configuration;

FIG. 5 is a schematic view of an alternative angular cartridge configuration;

in the drawings: 1. a sample cartridge; 11. a box body; 111. a first rib; 12. a cover body; 121. a vertical plate; 122. a second rib; 123. mounting blocks; 124. mounting a step; 14. a filter basket; 141. a top surface; 142. a first side; 143. a second side surface; 144. a third side; 145. a fourth side; 146. a filtration pore; 2. a homogenizer; (ii) a 4. A balancing liquid container; 5. a pump assembly; 51. a first peristaltic pump; 52. a second peristaltic pump; 6. a piping system; 7. a centrifuge assembly; 71. a motor; 72. a rotating arm; 73. a centrifugal pan; 731. a first housing; 732. a second housing; 733. a second filter membrane; 734. a collection chamber; 735. a waste fluid chamber; 736. a liquid inlet; 737. a liquid outlet; 8. an anaerobic system; 81. an inert gas bottle; 82. an air pump; 83. a valve; 9. a rotary joint; 10. a waste liquid tank; 110. a waste liquid bag.

Detailed Description

The present invention will be further described with reference to the following embodiments. Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.

The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", etc., based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of the description, but it is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation and operate, and therefore the terms describing the positional relationship in the drawings are only used for illustrative purposes and are not to be construed as limiting the present patent, and it is possible for one of ordinary skill in the art to understand the specific meaning of the above terms according to the specific situation.

Examples

Fig. 1 to 5 show an embodiment of a biological sample separation and extraction device according to the present invention, which includes a sampling box 1, a homogenizer 2, a balancing liquid container 4, a pump assembly 5, a piping system 6, a centrifugal assembly 7, and an anaerobic system 8 for creating an anaerobic environment for the separation and extraction device, wherein the sampling box 1 is disposed in the homogenizer 2, the centrifugal assembly 7 includes a centrifugal disk 73 communicated with the piping system 6, the sampling boxes 1, 3, the balancing liquid container 4, and the centrifugal disk 73 are communicated with each other via the piping system 6, the pump assembly 5 is connected to the piping system 6 to provide power for pumping liquid in the piping system 6, the sampling box 1 includes a box body 11, a cover 12, and a filter basket 14, the box body 11 is detachably connected to the cover 12, the filter basket 14 is connected to the cover 12, and the filter basket 14 is disposed in the box body 11, the homogenizer 2 of this embodiment may be an oscillating disk, a swing homogenizer, or a beater homogenizer, and may also be used as an auxiliary for improving the homogenization effect of a sample. It should be noted that, the pipeline system 6 of the present embodiment may be provided with a plurality of valves to control the flow of the liquid, and the plurality of valves may all adopt electromagnetic valves to realize the automatic control of the valves at various positions.

In the implementation of the embodiment, before the biological sample is separated, an anaerobic environment is formed in the separation and extraction device by adopting the anaerobic system 8, and the whole pipeline system 6 is closed; the sample is diluted in the balance liquid and is contained in the sampling box 1, the sample is crushed and homogenized by the homogenizer 2, and then is filtered and pumped to the centrifugal disc 73 under the action of the pump assembly 5, and the centrifugal disc 73 centrifuges to separate impurities from the biological sample; under the action of the pump assembly 5, the balance liquid is pumped to a centrifugal disc for washing and filtering and repeated for a plurality of times to improve the separation quality of the biological sample. The invention pumps liquid through the pump assembly 5 and separates and extracts biological samples through the centrifugal disc 73, thereby being sanitary and environment-friendly and being capable of extracting biological samples quickly and efficiently; in addition, the invention separates and extracts the biological sample in the anaerobic environment, which can effectively avoid the adverse effect of oxygen on the quality of the biological sample. The embodiment is particularly suitable for separating and extracting intestinal flora samples, and certainly can also be suitable for separating and extracting cell samples with activities not affected by anaerobic environment, such as tumor cells, cancer cells and the like.

In one embodiment, the filter basket 14 is disposed near an inner wall of one side of the box body 11, the filter basket 14 has a top surface 141, and a first side surface 142, a second side surface 143, a third side surface 144, and a fourth side surface 145 respectively connected to the top surface 141, the first side surface 142, the second side surface 143, the third side surface 144, and the fourth side surface 145 are sequentially connected end to end, the first side surface 142 is disposed near the inner wall of the box body, the top surface 141, the first side surface 142, the second side surface 143, and the fourth side surface 145 are all provided with filter holes 146, and the third side surface 144 is not provided with filter holes, as shown in fig. 4 and 5. The larger sized solid particles are retained within the filter cartridge by the filtering action of the filter basket 14, while the smaller sized solid particles and liquid flow to the next stage for fine filtration. In this embodiment, the side of the filter basket 14 away from the inner wall of the box body 11 is not provided with filter holes, so that the larger solid particles trapped in the sampling box 1 can smoothly fall down to the bottom of the sampling box along the side to prevent the solid particles from accumulating and blocking the filter holes. The pore size of the filtration pores is 0.8mm, but the pore size is not intended to limit the present invention, and the pore size may be changed depending on the filtration object.

In one embodiment, the inner wall of the box 11 is provided with a plurality of first ribs 111, and the inner wall of the cover 12 is connected with a vertical plate 121 and a plurality of second ribs 122, as shown in fig. 4 and 5. Second bead 122, riser 121, first bead 111 play the effect the same, all play vortex and broken effect, mutually support in order to possess better vortex and crushing effect with first bead 111 and riser 121.

In one embodiment, the cover 12 is provided with a mounting block 123, the mounting block 123 is connected with the filter basket 14 in a sealing manner, and the mounting block 123 is provided with a mounting step 124 for mounting the first filter membrane, as shown in fig. 4 and 5. In this embodiment, the two-stage installation steps 124 may be arranged to install two layers of first filter membranes with different apertures, or the multi-stage installation steps 124 may be arranged to install multiple layers of first filter membranes with different apertures, where the aperture of the first filter membrane decreases from top to bottom, and the filtering precision is gradually decreased from coarse to fine. When the embodiment is used for extracting intestinal flora from human excrement, two layers of first filter membranes are adopted for filtering, and the pore diameters of the two layers of first filter membranes are respectively 80 microns and 0.5 micron. In one embodiment, the anaerobic system 8 includes an inert gas bottle 81, an air pump 82 and a valve 83, the air pump 82 is connected between the inert gas bottle 81 and the pipeline system 6, and the valve 83 is disposed between the air pump 82 and the pipeline system 6. In this embodiment, the inert gas in the inert gas bottle 81 is fed into the pipeline system 6 and the sample box 1 and the equilibrium liquid container 4 by the air pump 82, so as to form an inert oxygen-free atmosphere in the separation and extraction device, thereby avoiding the influence of oxygen on the biological sample.

In another embodiment, the anaerobic system 8 may not be a mechanical improvement, but may be a biomass modification, in which a substance consuming oxygen is added into the sample container 1, and the filtered fluid flows in the pipeline system 6, and the substance consuming oxygen consumes oxygen in the pipeline system 6 to form an anaerobic environment, so as to avoid adverse effects of oxygen on the biological sample product.

In one embodiment, the pump assembly 5 comprises a first peristaltic pump 51 and a second peristaltic pump 52, and the tubing system 6 comprises a first tubing circuit connected between the sample cartridge 1 and the centrifuge disk 73 and a second tubing circuit connected between the equilibration fluid container 4 and the centrifuge disk 73, the first peristaltic pump 51 and the second peristaltic pump 52 being provided in the first tubing circuit and the second tubing circuit, respectively. In this embodiment, the first peristaltic pump 51 pumps the sample solution in the sample box 1 to the centrifugal disc 73, and the centrifugal disc 73 performs centrifugal separation; the second peristaltic pump 52 pumps the balancing solution in the balancing solution container 4 to the centrifugal disc 73 to wash and then centrifuge the obtained sample, and the process is repeated for multiple times, so that the quality of the obtained biological sample can be effectively improved.

In another embodiment, besides the first peristaltic pump 51 and the second peristaltic pump 52 as the pump assembly 5, a syringe pump may be used as the pump assembly, and the syringe pump first sucks the liquid in the sample box 1 or the liquid in the equilibrium liquid container into the syringe pump through the control valve, and then pushes the liquid in the syringe pump out to the centrifugal disk through the control valve.

In one embodiment, the piping system 6 comprises a first pipe and a second pipe which are arranged in communication, the sampling box 1 and the balancing liquid container 4 are both communicated with the first pipe, the centrifugal disc 73 is communicated with the end of the second pipe, and a rotary joint 9 is arranged at the joint of the first pipe and the second pipe. The rotary joint 9 is provided to prevent the centrifugal disk 73 from causing the winding of the pipe line when centrifugally rotated. The rotary joint 9 includes a first connection portion and a second connection portion rotatably connected, the first connection portion is fixed and connected to the first pipeline, the second connection portion is rotatable relative to the first connection portion, and a rotation axis thereof is parallel to a rotation axis of the rotary arm 72. Thus, during the centrifugal rotation, the second duct and the centrifugal disc 73 rotate as a unit, and the second duct does not intertwine during rotation.

In one embodiment, the centrifugal assembly 7 comprises a motor 71 and a rotating arm 72, the rotating arm 72 is connected to an output end of the motor 71, and the centrifugal disc 73 is connected to an end of the rotating arm 72 away from the motor 71. In this embodiment, the motor 71 drives the rotating arm 72 to rotate around the axis of the motor 71, the centrifugal disc 73 rotates along the circumference to perform centrifugal separation of the filtrate of the biological sample, and the biological sample is accumulated at one end of the centrifugal disc 73 away from the axis of the motor 71. In this embodiment, the centrifugal discs 73 may be two sets, two sets of centrifugal discs 73 are respectively disposed at two ends of the rotating arm 72, and the two sets of centrifugal discs 73 are used for centrifugal separation at the same time, so as to improve the separation efficiency of the biological sample. Of course, the number of the centrifugal discs 73 in this embodiment is not limited to two, and a plurality of sets of centrifugal discs 73 may be provided to further improve the separation efficiency of the biological samples.

In one embodiment, the centrifugal plate 73 comprises a first housing 731, a second housing 732, and a second filter membrane 733 connected between the first housing 731 and the second housing 732, wherein a collection chamber 734 is formed between the first housing 731 and the second filter membrane 733, a waste liquid chamber 735 is formed between the second housing 732 and the second filter membrane 733, the collection chamber 734 is connected to a liquid inlet 736 communicated with the pipe system 6, the waste liquid chamber 735 is connected to a liquid outlet 737, and the aperture of the second filter membrane 733 is smaller than the diameter of the biological sample, as shown in fig. 2. In this embodiment, when the liquid to be filtered enters the collecting cavity 734 from the liquid inlet 736, under the action of centrifugal force and liquid propelling force, the filtered waste liquid enters the waste liquid cavity 735 through the second filter membrane 733 and is discharged through the liquid outlet 737, in this embodiment, an annular waste liquid tank 10 may be disposed below the liquid outlet 737, and the waste liquid tank 10 is located below the rotation path of the liquid outlet 737 to collect the waste liquid; during the centrifugation process, the target biological sample is blocked by the second filter membrane 733 and is thrown away from the second filter membrane 733 by the centrifugal force to settle in the collection chamber 734, so that after the centrifugation is finished, the operator can directly take away the centrifugal plate 73 to obtain the biological sample. The second filter film 733 is hydrophilic.

In another embodiment, to ensure a strict anaerobic environment of the separation and extraction apparatus, a waste liquid bag 110 is connected to the liquid outlet 737 for collecting waste liquid, as shown in fig. 3. So set up, except forming sealed environment, still can avoid the waste liquid to cause the pollution to the environment.

In one embodiment, the collection chamber 734 is an inverted cone shaped structure having a cross-sectional area that decreases away from the second filter membrane 733. The cross-sectional shape of the collection chamber 734 may be square or circular, and since the centrifugal force farthest from the spindle is the largest, the cells may be better deposited in the collection chamber 734, so that the cells are not easily blocked to the holes of the second filter membrane 733, thereby ensuring smooth filtration.

In one embodiment, when the present embodiment is used for separating and extracting intestinal flora, the pore size of the second filter 733 is 0.1 μm to 0.45 μm, the centrifugation speed of the centrifugation assembly 7 is 7000rpm to 15000rpm, and the centrifugation acceleration is 3000g to 30000g. The size of the intestinal bacteria is above 0.22 μm, the pore size of the second filter membrane 733 is smaller than that of the intestinal bacteria to retain the intestinal bacteria in the collecting cavity 734, and at the same time, the pore size of the second filter membrane 733 cannot be too small, and the second filter membrane 733 with too small pore size is easy to reduce the flow rate of the waste liquid from the collecting cavity 734 to the waste liquid cavity 735. In addition, as the size of the intestinal bacteria is much smaller than that of the cells, the centrifugal speed for separating the intestinal bacteria is set to 7000 rpm-15000 rpm so as to obtain better centrifugal effect; when the present embodiment is used for the separation and extraction of tumor cells, the pore size of the second filter 733 and the centrifugation speed of the centrifugation component 7 can be adaptively changed according to the size of tumor cells.

In the detailed description of the embodiments, various technical features may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. This need not be, nor should it be exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. A biological sample separation and extraction device is characterized in that: the device comprises a sampling box (1), a homogenizer (2), a balancing liquid container (4), a pump assembly (5), a pipeline system (6), a centrifugal assembly (7) and an anaerobic system (8) for creating an anaerobic environment for a separation and extraction device, wherein the sampling box (1) is arranged in the homogenizer (2), the centrifugal assembly (7) comprises a centrifugal disc communicated with the pipeline system (6), the sampling box (1), the balancing liquid container (4) and the centrifugal disc are communicated with each other through the pipeline system (6), and the pump assembly (5) is connected to the pipeline system (6) to provide power for pumping liquid in the pipeline system (6); sampling box (1) includes box body (11), lid (12), box body (11) and lid (12) can be dismantled and be connected, lid (12) are connected with filter basket (14) just filter basket (14) are located box body (11).

2. The biological sample separating and extracting device according to claim 1, wherein the filter basket (14) is disposed near an inner wall of one side of the box body (11), the filter basket (14) is provided with a top surface (141) and a first side surface (142), a second side surface (143), a third side surface (144) and a fourth side surface (145) which are respectively connected with the top surface (141), the first side surface (142), the second side surface (143), the third side surface (144) and the fourth side surface (145) are sequentially connected end to end, the first side surface (142) is disposed near the inner wall of the box body, the top surface (141), the first side surface (142), the second side surface (143) and the fourth side surface (145) are all provided with filter holes (146), and the third side surface (144) is not provided with filter holes.

3. The device for separating and extracting biological samples according to claim 1, wherein the inner wall of the box body (11) is provided with a plurality of first ribs (111), and the inner wall of the cover body (12) is connected with a vertical plate (121) and a plurality of second ribs (122).

4. The device for separating and extracting biological samples as claimed in claim 1, wherein the cover body (12) is provided with a mounting block (123), the mounting block (123) is connected with the filter basket (14) in a sealing way, and the mounting block (123) is provided with a mounting step (124) for mounting the first filter membrane.

5. The device for separating and extracting biological samples according to claim 1, wherein the anaerobic system (8) comprises an inert gas bottle (81), an air pump (82) and a valve (83), the air pump (82) is communicated between the inert gas bottle (81) and the pipeline system (6), and the valve (83) is arranged between the air pump (82) and the pipeline system (6).

6. The device for separating and extracting biological samples according to claim 1, wherein the pump assembly (5) comprises a first peristaltic pump (51) and a second peristaltic pump (52), the tubing system (6) comprises a first tubing circuit connected between the sample cartridge (1) and the centrifugal disk (73) and a second tubing circuit connected between the equilibration fluid container (4) and the centrifugal disk (73), and the first peristaltic pump (51) and the second peristaltic pump (52) are respectively arranged in the first tubing circuit and the second tubing circuit.

7. The biological sample separation and extraction device according to claim 1, wherein the pipeline system (6) comprises a first pipeline and a second pipeline which are arranged in a communication manner, the sample cartridge (1) and the balancing liquid container (4) are both communicated with the first pipeline, the centrifugal disc (73) is communicated with the end part of the second pipeline, and a rotary joint (9) is arranged at the joint of the first pipeline and the second pipeline.

8. The biological sample separation and extraction device according to any one of claims 1 to 7, characterized in that the centrifugal assembly (7) comprises a motor (71) and a rotating arm (72), the rotating arm (72) is connected to an output end of the motor (71), and the centrifugal disc (73) is connected to an end of the rotating arm (72) far away from the motor (71).

9. The device for separating and extracting biological samples according to claim 8, wherein the centrifugal plate (73) comprises a first housing (731), a second housing (732), and a second filter membrane (733) connected between the first housing (731) and the second housing (732), a collecting chamber (734) is formed between the first housing (731) and the second filter membrane (733), a waste liquid chamber (735) is formed between the second housing (732) and the second filter membrane (733), the collecting chamber (734) is connected to a liquid inlet (736) communicated with the duct system (6), the waste liquid chamber (735) is connected to a liquid outlet (737), and the aperture of the second filter membrane (733) is smaller than the diameter of the biological sample.

10. The device for separating and extracting biological samples according to claim 9, wherein the collecting chamber (734) is an inverted cone structure, the cross-sectional area of the inverted cone structure gradually decreases in a direction away from the second filter membrane (733); the aperture of the second filter membrane (733) is 0.1-0.45 μm, the centrifugation speed of the centrifugation component (7) is 7000-15000 rpm, and the centrifugation acceleration is 3000-30000 g.

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