CN212904920U

CN212904920U - Stem cell separation and detection device

Info

Publication number: CN212904920U
Application number: CN202021416985.3U
Authority: CN
Inventors: 赵晓彤; 袁牧
Original assignee: Weihai Jiansheng Biotechnology Co ltd
Current assignee: Weihai Jiansheng Biotechnology Co ltd
Priority date: 2020-07-17
Filing date: 2020-07-17
Publication date: 2021-04-06
Anticipated expiration: 2030-07-17

Abstract

The utility model belongs to the technical field of stem cell detection and separation, and particularly discloses a stem cell separation and detection device, which comprises a box body, wherein the bottom end in the box body is provided with a rotating motor, the output end of the rotating motor is provided with a rotating shaft, the top end of the rotating shaft is provided with a clamping seat, a cup body is clamped in the clamping seat, a thermostatic bath is arranged on the table-board at the top of the box body, and the top of the thermostatic bath is provided with sealing glass through a rotating shaft, one end of the thermostatic bath is provided with a connecting pipe, the bottom end of the thermostatic bath is provided with an electric valve, the bottom end of the electric valve is provided with a sleeve, one side of the thermostatic bath is provided with a support frame, and support frame one side is provided with the connecting rod, is provided with the microscope on the connecting rod, and the microscope top is provided with the camera, and support frame one side is provided with computer display, is provided with total controller on the side of box one end, and bottom half four corners position all is provided with the supporting pad, and the box front surface is provided with the chamber door. The utility model discloses can go out stem cell through high-speed centrifuge flash separation, the practicality is strong.

Description

Stem cell separation and detection device

Technical Field

The utility model relates to a stem cell separation detection device belongs to stem cell and detects separation technical field.

Background

Stem cells are original cells, namely original undifferentiated cells with self-replication capacity and multidirectional differentiation potential, play a key role in human epidermis and hematopoietic system, most of the prior stem cell separation still adopts manual separation, the labor intensity is high, the efficiency is low, the consumed time in the process of extracting the stem cells is long, the activity of the cells is not maintained, and the extracted stem cells are not pure enough due to the fact that plasma pollution is easily caused during extraction and separation.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a stem cell separation detection device solves the problem of proposing among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a stem cell separation and detection device comprises a box body, wherein a high-speed centrifuge is arranged at the bottom end in the box body, a clamping seat is arranged at the top end of the high-speed centrifuge, grooves are formed in two ends of the clamping seat, a fixing device is arranged in each groove, a cup body is clamped in the clamping seat, clamping blocks are arranged at two ends of each cup body, the clamping blocks at two ends are respectively arranged in the corresponding grooves of the clamping seat, a thermostatic bath is arranged on a table board at the top of the box body, sealing glass is arranged at the top of the thermostatic bath through a rotating shaft and is rotationally connected with one end of the thermostatic bath, a connecting pipe is arranged at one end of the thermostatic bath and is communicated with the interior of the thermostatic bath, an electric valve is arranged at the bottom end of the thermostatic bath, a filter screen is arranged in the electric valve, a sleeve is arranged, one side that the connecting pipe was kept away from to the constant temperature tank is provided with the support frame, the one end that is close to the constant temperature tank on the support frame has been seted up the slide, and support frame one side is provided with the connecting rod, the connecting rod passes through the slider setting in the slide of support frame, and connecting rod and support frame sliding connection, it is provided with elevator motor to be close to support frame one end on the connecting rod, be provided with the microscope on the connecting rod, and the microscope runs through the connecting rod upper and lower extreme, microscope top both ends are provided with the supporting shoe, and both ends supporting shoe top is provided with the camera, the support frame is kept away from constant temperature tank one side and is provided with computer display, be provided with total controller on the side of box one end, bottom half four corners position.

Preferably, the computer display is electrically connected with the camera through a connecting wire.

Preferably, a heater is arranged on the inner wall of one end of the thermostatic bath.

Preferably, the fixing device comprises a spring A and a connecting block, the spring A is arranged in the groove, and two ends of the spring A are respectively connected with the inner wall of one side of the groove and the connecting block.

Preferably, the inner walls of the two ends of the clamping seat are provided with vibrators.

Preferably, the master controller is electrically connected with the lifting motor, the high-speed centrifuge, the electric valve, the vibrator and the heater.

Preferably, the bottom end of the electric valve is provided with a circle of limiting ring.

Preferably, the bottom end of the sleeve is provided with a circular groove, the top end of the cup body is embedded with the circular groove, and the width of the circular groove is larger than the thickness of the cup body.

Preferably, the supporting pad contains screw rod, fixation nut, spring B and vibration-damping rubber pad, be provided with the screw rod on the vibration-damping rubber pad, and screw rod one end passes the bottom half and sets up in the box, the screw rod top is provided with fixation nut, and fixation nut and screw rod threaded connection, spring B sets up on the screw rod between vibration-damping rubber pad and the bottom half.

Preferably, a liquid outlet is formed in the top end of one side of the cup body, a piston plate is arranged in the cup body, leakproof rubber is arranged on the outer circle of the piston plate, the piston plate and the leakproof rubber are both in sliding connection with the cup body, and the cup body is hollow.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model belongs to the technical field of stem cell detection and separation, in particular to a stem cell separation and detection device, which can separate impurities from an extracted substance through a filter screen, thereby improving the purity of the extracted substance; the cup body can be firmly fixed in the clamping seat by the arranged fixing device, so that the cup body is prevented from falling off the clamping seat during rotation and vibration; the supporting pads can reduce the vibration generated when the centrifugal machine works so as to improve the stability of the machine; the circular groove on the sleeve is embedded with the top end of the cup body, so that the blood in the cup body can be prevented from overflowing from the cup opening in the centrifugal and vibration processes; the arranged sealing glass can prevent other objects from entering the thermostatic bath while observing by a microscope; the arranged heater can play a constant temperature role, and the survival rate of stem cells of the extract in the constant temperature tank can be improved; the arranged vibrator can vibrate the cup body, so that the stem cell separation speed is increased; the piston plate is arranged to separate stem cells, red blood cells and plasma by moving in the cup body.

Drawings

Fig. 1 is a sectional elevation view of the present invention;

fig. 2 is a front view of the present invention;

FIG. 3 is a schematic view of the connection relationship between the damping device and the U-shaped swivel body of the present invention;

FIG. 4 is a schematic view of the connection relationship between the supporting pad and the box body;

fig. 5 is a cross-sectional view of the bottom of the sleeve of the present invention;

in the figure: 1. a box body; 2. a high speed centrifuge; 3. a clamping seat; 4. a groove; 5. a fixing device; 51. A spring A; 52. connecting blocks; 6. a cup body; 7. a clamping block; 8. a thermostatic bath; 9. sealing glass; 10. a connecting pipe; 11. an electrically operated valve; 12. filtering with a screen; 13. a sleeve; 14. a support frame; 15. leakage-proof rubber; 16. a connecting rod; 17. a slider; 18. a slideway; 19. a lifting motor; 20. a microscope; 21. a support block; 22. a camera; 23. a computer display; 24. a master controller; 25. a support pad; 251. a screw; 252. Fixing a nut; 253. a spring B; 254. a shock-absorbing rubber pad; 26. a box door; 27. a connecting wire; 28. A heater; 29. a vibrator; 30. a limiting ring; 31. a circular groove; 32. a liquid outlet; 33. a piston plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Referring to fig. 1-5, the present invention provides a technical solution: a stem cell separation and detection device comprises a box body 1, wherein a high-speed centrifuge 2 is arranged at the bottom end in the box body 1, a clamping seat 3 is arranged at the top end of the high-speed centrifuge 2, grooves 4 are respectively formed in two ends of the clamping seat 3, a fixing device 5 is arranged in each groove 4, a cup body 6 is clamped in each clamping seat 3, fixture blocks 7 are respectively arranged at two ends of each cup body 6, the fixture blocks 7 at two ends are respectively arranged in the corresponding grooves 4 of each clamping seat 3, a thermostatic bath 8 is arranged on a table top at the top of the box body 1, sealing glass 9 is arranged at the top of the thermostatic bath 8 through a rotating shaft, the sealing glass 9 is rotatably connected with one end of the thermostatic bath 8, a connecting pipe 10 is arranged at one end of the thermostatic bath 8, the connecting pipe 10 is connected and communicated with the thermostatic bath 8, an electric valve 11 is arranged at the bottom, the bottom end of the electric valve 11 is provided with a sleeve 13, the electric valve 11 penetrates through a center hole of the sleeve 13 to be connected with the sleeve 13 in a sliding manner, one side of the thermostatic bath 8, which is far away from the connecting pipe 10, is provided with a support frame 14, one end, which is close to the thermostatic bath 8, of the support frame 14 is provided with a slide way 18, one side of the support frame 14 is provided with a connecting rod 16, the connecting rod 16 is arranged in the slide way 18 of the support frame 14 through a slide block 17, the connecting rod 16 is connected with the support frame 14 in a sliding manner, one end, which is close to the support frame 14, of the connecting rod 16 is provided with a microscope 20, the microscope 20 penetrates through the upper end and the lower end of the connecting rod 16, two ends of the top of the microscope 20 are provided with support blocks 21, the tops of the two support blocks 21 are, supporting pads 25 are arranged at four corners of the bottom of the box body 1, a box door 26 is arranged on the front surface of the box body 1 through hinges, and the box door 26 is rotatably connected with the box body 1.

Further, the computer display 23 is electrically connected to the camera 22 through a connection line 27.

Further, a heater 28 is disposed on an inner wall of one end of the thermostatic bath 8.

Further, the fixing device 5 comprises a spring A51 and a connecting block 52, the spring A51 is arranged in the groove 4, and two ends of the spring A51 are respectively connected with the inner wall of one side of the groove 4 and the connecting block 52.

Further, the inner walls of the two ends of the clamping seat 3 are provided with vibrators 29.

Further, the general controller 24 is electrically connected with the lifting motor 19, the high-speed centrifuge 2, the electric valve 11, the vibrator 29 and the heater 28.

Further, a circle of limiting ring 30 is arranged at the bottom end of the electric valve 11.

Further, the bottom end of the sleeve 13 is provided with a circular groove 31, the top end of the cup body 6 is engaged with the circular groove 31, and the width of the circular groove 31 is greater than the thickness of the cup body 6.

Further, the support pad 25 includes a screw 251, a fixing nut 252, a spring B253 and a shock-absorbing rubber pad 254, the shock-absorbing rubber pad 254 is provided with the screw 251, one end of the screw 251 penetrates through the bottom of the box 1 and is disposed in the box 1, the top end of the screw 251 is provided with the fixing nut 252, the fixing nut 252 is in threaded connection with the screw 251, and the spring B253 is disposed on the screw 251 between the shock-absorbing rubber pad 254 and the bottom of the box 1.

Further, a liquid outlet 32 is arranged at the top end of one side of the cup body 6, a piston plate 33 is arranged in the cup body 6, a circle of the outer side of the piston plate 33 is provided with leakage-proof rubber 15, the piston plate 33 and the leakage-proof rubber 15 are both in sliding connection with the cup body 6, and the cup body 6 is hollow.

The working principle is as follows: the utility model relates to a stem cell separation detection device, during operation, firstly, a box door 26 is opened, a sleeve 13 is moved upwards, then a clamping block 7 arranged on a cup body 6 is aligned with a groove 4 on a clamping seat 3, the clamping block 7 and the groove 4 are mutually embedded to prevent the cup body 6 from falling off the clamping seat 3, thereby the stability of the cup body 6 is improved, then the sleeve 13 is moved downwards, a circular groove 31 at the bottom of the sleeve 13 is embedded with a cup mouth to prevent the extracted blood from leaking from the cup mouth, then the extracted blood flows into a constant temperature groove 8 through a connecting pipe 10, a heater 28 arranged in the constant temperature groove 8 is heated at the moment, when the set temperature is reached, the heater 28 stops heating, the extracted blood in the constant temperature groove 8 is amplified through a microscope 20 until the extracted blood is clearly observed, a lifting motor 19 arranged can vertically move on a support frame 14 to adjust the object distance of the microscope 20, the camera 22 shoots again, the camera 22 transmits the picture to the computer display 23 for observation and storage after shooting is finished, then the electric valve 11 is opened through the master controller 24, the blood flows into the filter screen 12, the filter screen 12 filters impurities of the extracted blood to ensure the purity of the extracted blood, the filtered blood flows into the cup body 6, then the separating liquid is added into the cup body 6 through the connecting pipe 10, the master controller 24 controls the high-speed centrifuge 2 to work, then the vibrator 29 vibrates the cup body 6 to accelerate the separation speed of the extracted blood, after the separation of the dry cells is finished, the vibrator 29 and the high-speed separator 2 stop working, the electric valve 11 is closed through the master controller 24, the blood is divided into a plasma layer, a dry cell layer and a red cell layer from top to bottom after standing, then the cup body 6 is taken out of the clamping seat 3, and then the box door 26 is closed, observing the upper plasma layer of the cup body 6 after being taken out, then placing a plasma storage cup in the liquid outlet 32, then pushing the piston plate 33 to move upwards in the cup body 6 to enable the plasma layer to flow out of the liquid outlet 32, when the plasma in the cup body 6 is completely separated, then the stem cell storage cup is placed in the liquid outlet 32, the piston plate 33 is pushed upwards, the stem cell layer completely flows into the stem cell storage cup through the liquid outlet 32, only the red blood cell layer remains in the cup body 6, the stem cell separation work is completed from this, high speed centrifuge 2 during operation, and the vibrations that high speed centrifuge 2 during operation brought can be cushioned to spring B253 on the supporting pad 25 of setting, promotes the stability of machine, and in the sealed glass 9 of setting can keep away debris and get into thermostatic bath 8 when microscope 20 observes, the heater 28 of setting can play homothermal effect in to thermostatic bath 8, can improve the stem cell survival rate of the interior extract product of thermostatic bath 8.

It is worth noting that: because the equipment that master controller matches is equipment commonly used, belongs to current mature technology, its electric connection relation and specific circuit structure are no longer repeated herein.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A stem cell separation detection device is characterized in that: comprises a box body (1), a high-speed centrifuge (2) is arranged at the bottom end in the box body (1), a clamping seat (3) is arranged at the top end of the high-speed centrifuge (2), grooves (4) are respectively arranged at two ends of the clamping seat (3), a fixing device (5) is arranged in each groove (4), a cup body (6) is clamped in each clamping seat (3), clamping blocks (7) are respectively arranged at two ends of each cup body (6), the clamping blocks (7) at the two ends are respectively arranged in the corresponding grooves (4) of the clamping seats (3), a thermostatic bath (8) is arranged on the table-board at the top of the box body (1), sealing glass (9) is arranged at the top of the thermostatic bath (8) through a rotating shaft, the sealing glass (9) is rotationally connected with one end of the thermostatic bath (8), a connecting pipe (10) is arranged at one end of the thermostatic bath (8), and the connecting pipe (10) is communicated with, the device is characterized in that an electric valve (11) is arranged at the bottom end of the thermostatic bath (8), a filter screen (12) is arranged in the electric valve (11), a sleeve (13) is arranged at the bottom end of the electric valve (11), the electric valve (11) penetrates through a center hole of the sleeve (13) to be in sliding connection with the sleeve (13), a support frame (14) is arranged on one side, away from the connecting pipe (10), of the thermostatic bath (8), a slide way (18) is arranged at one end, close to the thermostatic bath (8), of the support frame (14), a connecting rod (16) is arranged on one side of the support frame (14), the connecting rod (16) is arranged in the slide way (18) of the support frame (14) through a sliding block (17), the connecting rod (16) is in sliding connection with the support frame (14), a lifting motor (19) is arranged at one end, close to the support, and microscope (20) run through connecting rod (16) upper and lower extreme, microscope (20) top both ends are provided with supporting shoe (21), and both ends supporting shoe (21) top is provided with camera (22), constant temperature tank (8) one side is kept away from in support frame (14) is provided with computer display (23), be provided with total controller (24) on the side of box (1) one end, box (1) bottom four corners position all is provided with supporting pad (25), box (1) positive surface is provided with chamber door (26) through the hinge, and chamber door (26) rotate with box (1) and be connected.

2. The stem cell separation and detection device according to claim 1, wherein: the computer display (23) is electrically connected with the camera (22) through a connecting wire (27).

3. The stem cell separation and detection device according to claim 1, wherein: and a heater (28) is arranged on the inner wall of one end of the thermostatic bath (8).

4. The stem cell separation and detection device according to claim 1, wherein: fixing device (5) contain spring A (51) and connecting block (52), spring A (51) set up in recess (4), and spring A (51) both ends are connected with recess (4) one side inner wall and connecting block (52) respectively.

5. The stem cell separation and detection device according to claim 1, wherein: and vibrators (29) are arranged on the inner walls of the two ends of the clamping seat (3).

6. The stem cell separation and detection device according to claim 1, wherein: and the master controller (24) is electrically connected with the lifting motor (19), the high-speed centrifuge (2), the electric valve (11), the vibrator (29) and the heater (28).

7. The stem cell separation and detection device according to claim 1, wherein: the bottom end of the electric valve (11) is provided with a circle of limiting ring (30).

8. The stem cell separation and detection device according to claim 1, wherein: the bottom end of the sleeve (13) is provided with a circular groove (31), the top end of the cup body (6) is embedded with the circular groove (31), and the width of the circular groove (31) is larger than the thickness of the cup body (6).

9. The stem cell separation and detection device according to claim 1, wherein: the supporting pad (25) comprises a screw rod (251), a fixing nut (252), a spring B (253) and a shock-absorbing rubber pad (254), the screw rod (251) is arranged on the shock-absorbing rubber pad (254), one end of the screw rod (251) penetrates through the bottom of the box body (1) and is arranged in the box body (1), the fixing nut (252) is arranged at the top end of the screw rod (251), the fixing nut (252) is in threaded connection with the screw rod (251), and the spring B (253) is arranged on the screw rod (251) between the shock-absorbing rubber pad (254) and the bottom of the box body (1).

10. The stem cell separation and detection device according to claim 1, wherein: cup (6) one side top is provided with liquid outlet (32), and is provided with piston plate (33) in cup (6), piston plate (33) outside round is provided with leak protection rubber (15), piston plate (33) and leak protection rubber (15) all with cup (6) sliding connection, and cup (6) are hollow form.