CN113398654A - Micro centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method - Google Patents
Micro centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method Download PDFInfo
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- CN113398654A CN113398654A CN202110575309.3A CN202110575309A CN113398654A CN 113398654 A CN113398654 A CN 113398654A CN 202110575309 A CN202110575309 A CN 202110575309A CN 113398654 A CN113398654 A CN 113398654A
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- centrifugal
- filter
- built
- filtrate
- vertical partition
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- 238000001914 filtration Methods 0.000 title claims abstract description 40
- 238000005192 partition Methods 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title abstract description 11
- 239000002245 particle Substances 0.000 claims abstract description 52
- 239000000706 filtrate Substances 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 239000011521 glass Substances 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 8
- 239000008187 granular material Substances 0.000 description 8
- 239000007787 solid Substances 0.000 description 6
- 238000000926 separation method Methods 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000005245 sintering Methods 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
- B01D36/04—Combinations of filters with settling tanks
- B01D36/045—Combination of filters with centrifugal separation devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/15—Filters with filtering elements which move during the filtering operation with rotary plane filtering surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D37/00—Processes of filtration
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/38—Treatment of water, waste water, or sewage by centrifugal separation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Mechanical Engineering (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention relates to a micro centrifugal filter tube with a built-in vertical partition plate and a centrifugal filtering method. The centrifugal filtration method comprises the following steps: 1) adding the solution to be separated into a cavity to be filtered in a centrifuge tube body; 2) placing the centrifugal filter pipe in a centrifugal machine, and starting the centrifugal machine to carry out centrifugal filtration; 3) after the centrifugal filtration is finished, sucking the filtrate in the filtrate cavity, and pouring out the residual precipitated particles in the cavity to be filtered. Compared with the prior art, the centrifugal filter pipe can be matched with a conventional centrifuge for convenient use, integrates the centrifugal function and the filtering function, can effectively separate clear liquid and precipitated particles, and is convenient for subsequent absorption of the separated clear liquid.
Description
Technical Field
The invention belongs to the technical field of solid-liquid separation, and relates to a micro centrifugal filter tube with a built-in vertical partition plate and a centrifugal filtering method.
Background
Currently, common techniques for separating liquids and precipitating particulate matter in laboratories include centrifugation techniques and filtration techniques.
The centrifugal technology is to utilize the strong centrifugal force generated by the high-speed rotation of an object to make suspended particles in a rotating body settle or float, so that some particles can be concentrated or separated from other particles. The suspension particles are cells, organelles, viruses, biological macromolecules and the like which are prepared into a suspension state. When the rotor of the centrifuge rotates at a high speed and the density of suspended particles is greater than that of surrounding media, the particles move away from the axis direction and are settled; if the density of the particles is lower than that of the surrounding medium, the particles move in the axial direction and float. Centrifuge tubes are often used in centrifugation techniques, and are tubular sample containers that may have a sealed lid or gland. After centrifugation, the solid particles and liquid in the centrifuge tube are separated.
The filtration technology is an operation that under the action of a driving force or other external force, liquid (or gas) in suspension (or containing solid particles) permeates through a medium, and the solid particles and other substances are intercepted by the filter medium, so that the solid and other substances are separated from the liquid (or gas). Filtration techniques are often used with filter plates through which solid particles and liquid are separated.
At present, when suspended particles in liquid are separated, a centrifugal technology or a filtering technology is often used independently, and the following defects exist: (1) when the centrifugation technology is used, the centrifuged liquid and the precipitated particles cannot be well separated or separated in the centrifugal tube, and more or less precipitated particle substances in the lower layer can be sucked when the liquid in the upper layer is sucked. (2) The filtration technology often needs to use other external power to improve the filtration effect, such as using a vacuum pump to pump and filter, which has high requirements on the device.
In view of the above problems, the prior art combines the centrifugal technology and the filtration technology, and designs a flow path of upper and lower structures by adopting a double pipe structure to promote solid-liquid separation (for example, publication No. CN205659797U, a high-speed centrifugal filtration pipe; publication No. CN204182492U, a centrifugal filtration pipe; publication No. CN205253381U, a centrifugal filtration pipe). However, such structures and flow paths cannot withstand large centrifugal forces, and thus separation and filtration effects are often not desirable.
Disclosure of Invention
The invention aims to provide a micro centrifugal filter tube with a built-in vertical partition plate and a centrifugal filtering method. The centrifugal filter pipe can be matched with a conventional centrifuge for convenient use, integrates the centrifugal function and the filtering function, can effectively separate clear liquid and precipitated particles, and facilitates subsequent absorption of the separated clear liquid.
The purpose of the invention can be realized by the following technical scheme:
the utility model provides a built-in vertical baffle's trace centrifugal filter pipe, this centrifugal filter pipe includes the centrifugal tube body of vertical setting and the filter of vertical setting in centrifugal tube body inside, is equipped with the filtration pore on this filter, the filter divide into the inside of centrifugal tube body and treat filter chamber and filtrating chamber, the inside of treating the filter chamber be equipped with the granule supporting shoe. During centrifugal filtration, liquid in the solution to be separated in the cavity to be filtered passes through the filter holes and enters the filtrate cavity, and the residual precipitated particles are remained in the cavity to be filtered, so that solid-liquid separation is realized. Simultaneously, because treat that the inside of filter chamber is equipped with the granule supporting shoe, remaining deposit the granule all be located the granule supporting shoe, raise remaining deposit the granule through the granule supporting shoe, avoid mixing with the filtrating of filter opposite side contaction again. The particle supporting block is of a solid structure, so that liquid infiltration is avoided.
Further, the top of the centrifugal pipe body is provided with an opening. The solution to be separated is added into the chamber to be filtered through the top opening of the centrifuge tube.
Furthermore, a sealing cover is detachably arranged at the opening at the top of the centrifugal pipe body.
Furthermore, a plurality of filter holes are uniformly formed in the filter plate, and the aperture of each filter hole is in a micron or millimeter level. The filter plate is connected with the side surface and the bottom of the centrifugal tube body.
Further, the bottom of the particle supporting block is connected with the bottom of the centrifugal pipe body, and the side faces of the particle supporting block are respectively connected with the side face of the centrifugal pipe body and the side face of the filter plate.
Further, the top surface of the particle supporting block is higher than the bottom of the filtrate cavity.
Furthermore, the centrifugal tube body, the filter plate and the particle supporting block are all made of glass. The glass material is easy to clean and reuse, and is easy to obtain and low in cost.
A centrifugal filtration method based on a microcentrifuge filter tube with a built-in vertical partition plate comprises the following steps:
1) adding the solution to be separated into a cavity to be filtered in a centrifuge tube body;
2) placing the centrifugal filter pipe in a centrifugal machine, and starting the centrifugal machine to carry out centrifugal filtration;
3) after the centrifugal filtration is finished, sucking the filtrate in the filtrate cavity, and pouring out the residual precipitated particles in the cavity to be filtered.
Further, in step 3), a pipette or a syringe is used for sucking the filtrate in the filtrate cavity.
Further, in step 3), after the centrifugal filtration is finished, the top surface of the particle supporting block is higher than the top surface of the filtrate in the filtrate chamber, and the remaining sediment particles in the chamber to be filtered are located on the particle supporting block.
Compared with the prior art, the invention has the following characteristics:
1) the centrifugal filter pipe integrates a centrifugal function and a filtering function, so that clear liquid and precipitated particles can be effectively separated, and the clear liquid after separation can be conveniently absorbed subsequently.
2) The inside of centrifugal pipe body is vertical to be equipped with the filter, changes the flow path of structure about among the prior art into, not only can bear great centrifugal force, sets up the granule supporting shoe through treating the inside of filter chamber simultaneously, has avoided the sediment granule after the filtration to contact the mixture again with the filtrating of filter opposite side, has excellent filter effect.
Drawings
FIG. 1 is a schematic sectional view of a centrifugal filter tube according to the present invention;
FIG. 2 is a schematic top view of the centrifugal filter tube (particle support block omitted) of the present invention;
the notation in the figure is:
1-centrifugal tube body, 2-filter plate, 3-to-be-filtered cavity, 4-filtrate cavity, 5-particle supporting block, 6-filtrate, 7-precipitated particle.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments. The present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, but the scope of the present invention is not limited to the following embodiments.
Example (b):
the microcentrifuge filter tube with the built-in vertical partition plate comprises a vertically-arranged centrifugal tube body 1 and a vertically-arranged filter plate 2 arranged inside the centrifugal tube body 1, wherein filter holes are formed in the filter plate 2, the filter plate 2 divides the inside of the centrifugal tube body 1 into a to-be-filtered cavity 3 and a filtrate cavity 4, and a particle supporting block 5 is arranged inside the to-be-filtered cavity 3.
Wherein, the top of the centrifugal tube body 1 is provided with an opening. The top opening of the centrifugal tube body 1 is detachably provided with a sealing cover.
A plurality of filter holes are uniformly arranged on the filter plate 2, and the aperture of the filter holes is in a micron or millimeter level. The bottom of the particle supporting block 5 is connected with the bottom of the centrifugal pipe body 1, and the side surface of the particle supporting block 5 is respectively connected with the side surface of the centrifugal pipe body 1 and the side surface of the filter plate 2. The top surface of the particle support block 5 is higher than the bottom of the filtrate chamber 4. The centrifugal tube body 1, the filter plate 2 and the particle supporting block 5 are all made of glass.
The centrifugal filtration method based on the centrifugal filtration pipe comprises the following steps:
(1) preparing a centrifugal pipe body 1 with a proper pipe diameter according to the capacity of a centrifugal machine, wherein the volume of the centrifugal pipe body is 20mL, 50mL, 100mL and the like; the filter plate 2 is connected with a proper filter plate in a sintering way according to the particle size of the particles needing to be centrifuged and filtered, and the pore diameter can be micron-sized or millimeter-sized.
(2) The solution to be separated is added into the chamber 3 to be filtered in the centrifugal tube body 1, and the liquid level can not exceed the height of the filter plate 2, so that the solution to be separated can not overflow the filter plate 2.
(3) Placing the centrifugal filter tube in a centrifuge, starting the centrifuge to carry out centrifugal filtration, and selecting proper rotating speed according to the properties of the solution to be separated, such as 800r/min, 1000r/min, 1200r/min and the like; centrifuging for 20min, 30min, 40min, etc.
(4) After the centrifugal filtration is finished, the top surface of the particle supporting block 5 is higher than the top surface of the filtrate 6 in the filtrate cavity 4, and the sediment particles 7 remaining in the filter cavity 3 are all positioned on the particle supporting block 5. The filtrate 6 in the filtrate chamber 4 is aspirated by a pipette or syringe for subsequent test analysis.
(5) And pouring out the residual precipitated particles 7 in the cavity 3 to be filtered, cleaning the centrifugal filter tube, and airing for later use.
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (10)
1. The utility model provides a micro-centrifugal filter pipe of built-in vertical baffle, its characterized in that, this centrifugal filter pipe include centrifugal body (1) and the vertical filter (2) of setting in centrifugal body (1) inside of vertical setting, be equipped with the filtration pore on this filter (2), filter (2) divide into the inside of centrifugal body (1) and treat filter chamber (3) and filtrate chamber (4), the inside of treating filter chamber (3) be equipped with particle support piece (5).
2. The microcentrifuge filter tube with the built-in vertical partition plate of claim 1, is characterized in that the top of the centrifugal tube body (1) is provided with an opening.
3. The microcentrifuge filter tube with the built-in vertical partition plate is characterized in that a sealing cover is detachably arranged at the top opening of the centrifugal tube body (1).
4. The microcentrifuge filter tube with the built-in vertical partition plate according to claim 1, is characterized in that a plurality of filter holes are uniformly arranged on the filter plate (2), and the pore diameter of the filter holes is in micron or millimeter level.
5. The microcentrifuge filter tube with the built-in vertical partition plate is characterized in that the bottom of the particle supporting block (5) is connected with the bottom of the centrifugal tube body (1), and the side surfaces of the particle supporting block (5) are respectively connected with the side surface of the centrifugal tube body (1) and the side surface of the filter plate (2).
6. The microcentrifuge filter tube with built-in vertical partition of claim 1, which is characterized in that the top surface of the particle supporting block (5) is higher than the bottom of the filtrate chamber (4).
7. The microcentrifuge filter tube with the built-in vertical partition plate according to claim 1, is characterized in that the centrifugal tube body (1), the filter plates (2) and the particle supporting block (5) are all made of glass.
8. A centrifugal filtration method based on a microcentrifuge filter tube with a built-in vertical partition plate according to any one of claims 1 to 7, which is characterized by comprising the following steps:
1) adding a solution to be separated into a chamber (3) to be filtered in the centrifugal tube body (1);
2) placing the centrifugal filter pipe in a centrifugal machine, and starting the centrifugal machine to carry out centrifugal filtration;
3) after the centrifugal filtration is finished, sucking the filtrate (6) in the filtrate cavity (4), and pouring out the residual precipitated particles (7) in the cavity (3) to be filtered.
9. The centrifugal filtration method of the microcentrifuge filter tube based on the built-in vertical partition plate is characterized in that in the step 3), a liquid suction device or a syringe is used for sucking the filtrate (6) in the filtrate cavity (4).
10. The centrifugal filtration method of the microcentrifuge filter tube based on the built-in vertical partition plate is characterized in that in the step 3), after the centrifugal filtration is finished, the top surface of the particle supporting block (5) is higher than the top surface of the filtrate (6) in the filtrate cavity (4), and the rest of the precipitated particles (7) in the cavity (3) to be filtered are positioned on the particle supporting block (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110575309.3A CN113398654B (en) | 2021-05-26 | 2021-05-26 | Micro-centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110575309.3A CN113398654B (en) | 2021-05-26 | 2021-05-26 | Micro-centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method |
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| Publication Number | Publication Date |
|---|---|
| CN113398654A true CN113398654A (en) | 2021-09-17 |
| CN113398654B CN113398654B (en) | 2023-11-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110575309.3A Active CN113398654B (en) | 2021-05-26 | 2021-05-26 | Micro-centrifugal filter pipe with built-in vertical partition plate and centrifugal filtering method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170001203A1 (en) * | 2015-07-03 | 2017-01-05 | Seven Juice Co., Ltd. | Centrifugal filtering device and method for operating the same |
| CN207770061U (en) * | 2017-11-28 | 2018-08-28 | 贵州开磷质量检测中心有限责任公司 | A kind of double-deck centrifuge tube for solid-liquid quick separating |
| CN109794366A (en) * | 2019-01-14 | 2019-05-24 | 上海化工研究院有限公司 | A kind of flexible type micro high speed centrifugation device of umbrella shape |
| CN111408428A (en) * | 2020-05-02 | 2020-07-14 | 南京大学 | Double-cavity centrifuge tube integrating functions of suspension separation and supernatant extraction |
-
2021
- 2021-05-26 CN CN202110575309.3A patent/CN113398654B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170001203A1 (en) * | 2015-07-03 | 2017-01-05 | Seven Juice Co., Ltd. | Centrifugal filtering device and method for operating the same |
| CN207770061U (en) * | 2017-11-28 | 2018-08-28 | 贵州开磷质量检测中心有限责任公司 | A kind of double-deck centrifuge tube for solid-liquid quick separating |
| CN109794366A (en) * | 2019-01-14 | 2019-05-24 | 上海化工研究院有限公司 | A kind of flexible type micro high speed centrifugation device of umbrella shape |
| CN111408428A (en) * | 2020-05-02 | 2020-07-14 | 南京大学 | Double-cavity centrifuge tube integrating functions of suspension separation and supernatant extraction |
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| Publication number | Publication date |
|---|---|
| CN113398654B (en) | 2023-11-28 |
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