CN216427281U

CN216427281U - Novel cell filtering centrifuge tube capable of being combined

Info

Publication number: CN216427281U
Application number: CN202122703273.0U
Authority: CN
Inventors: 孙巍群; 赵振波; 夏信群; 叶大林
Original assignee: Zhejiang Tailin Medical Engineering Co ltd
Current assignee: Zhejiang Tailin Medical Engineering Co ltd
Priority date: 2021-11-03
Filing date: 2021-11-03
Publication date: 2022-05-03
Anticipated expiration: 2031-11-03

Abstract

The utility model discloses a novel cell filtration centrifuging tube that can make up, including collecting pipe and pipe cap, be equipped with the filter tube between collecting pipe and the pipe cap, the filter tube lower extreme is connected with the upper end of collecting pipe, and filter tube upper end and pipe cap releasable connection are equipped with millipore filtration membrane in the collecting pipe, and one side of millipore filtration membrane is equipped with through the breach. The utility model provides a novel cell filtration centrifuging tube that can make up washes the filtration many times through increasing, has reduced required centrifugation number of times of isolation cell and time, has reduced the loss of centrifugation back cell.

Description

Novel cell filtering centrifuge tube capable of being combined

Technical Field

The utility model belongs to the technical field of cell centrifuging tube technique and specifically relates to a novel cell filtration centrifuging tube that can make up is related to.

Background

In research in the field of life science, a separation operation of a biological sample using a centrifugal separation technique is the most basic operation. In operation, a centrifugal machine generates huge centrifugal force so as to completely separate micro-particle substances and supernatant in the biological sample. During cell recovery, for cells in the frozen stock solution, a centrifuge is usually used to settle the cells at the bottom to form a precipitate, and the supernatant is aspirated. However, in the operation process, the loss of the bottom cells is often caused by the overlarge action range of manual operation, which is unavoidable, and thus the collection of the frozen cells is greatly influenced. And because the cells are centrifuged for many times, the loss is brought to the number of the cells, the experimental time is increased, and the experimental efficiency is influenced. In addition, in the purification and filtration of biological samples, the filter screens with different pore sizes are generally used for separating and purifying particles with different diameters, but the filter screens are often required to be replaced repeatedly due to complex sample components, so that the loss of the sample is caused, the pollution risk is also caused, and the experiment efficiency is reduced. Therefore, it is necessary to design a new cell filtration centrifuge tube that can be combined to solve the above problems.

Disclosure of Invention

The utility model discloses an overcome among the prior art not enough of cell recovery in-process because of the cell quantity reduction that causes easily behind the cell centrifugation, provide a novel cell filtration centrifuging tube that can make up, wash the filtration many times through increasing, reduced the required centrifugation number of times of isolation cell and time, reduced the loss of centrifugation back cell.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a novel cell filtration centrifuging tube that can make up, includes collecting pipe and pipe cap, be equipped with the filter tube between collecting pipe and the pipe cap, the filter tube lower extreme is connected with the upper end of collecting pipe, and filter tube upper end and pipe cap releasable connection are equipped with millipore filtration membrane in the filter tube, and one side of millipore filtration membrane is equipped with through the breach.

Among the above-mentioned technical scheme, the filter tube sets the microfiltration membrane in multiple apertures such as 1um, 5um or 10um, can select the filter tube in different apertures as required, and microfiltration membrane adopts nylon material or nitrocellulose material, and microfiltration membrane occupies about half of the cross-sectional area of the body of filtering, and remaining part and body inner wall form the breach that passes through of adding solution, and the cell instrument of getting can pass through the breach and take the cell. The filter pipe can be connected with the pipe cap through threads to form a closed container. The collecting pipe can be connected with the filter tube, the cell suspension can be washed and filtered for a plurality of times before centrifugation, then the tube cap is covered for centrifugal operation, the supernatant is poured out after centrifugation, and the microporous filter membrane can avoid the cells to be poured out. According to the technical scheme, the number of times of centrifugation and the time required for separating cells are reduced by increasing the number of times of washing and filtering, and the loss of the centrifuged cells is reduced. The gas can smoothly enter the collecting pipe through the notch, so that the pouring speed is increased.

Preferably, the lower end of the filter pipe is detachably and hermetically connected with the upper end of the collecting pipe, and the upper end of the filter pipe is detachably and hermetically connected with the pipe cap. The collecting tube can be connected with the tube cap through threads and used as a conventional centrifugal tube. The detachable sealing connection can be a threaded connection with a sealing effect, a clamping connection and other connection structures.

Preferably, a sealing ring is arranged between the filtering pipe and the collecting pipe. The structure can increase the sealing effect between the filter pipe and the collecting pipe.

Preferably, a sealing ring is arranged between the upper end of the filter pipe and the pipe cap. The structure can increase the sealing effect between the upper end of the filter pipe and the pipe cap.

Preferably, the number of the filter pipes is 2, 2 filter pipes are stacked up and down, the 2 filter pipes are in detachable sealing connection, and the aperture of the micro-filtration membrane in the filter pipe above is larger than that of the micro-filtration membrane in the filter pipe below. The aperture of the micro-filtration membrane in the upper filtration tube is 30-50um, 60-80um or 80-120um, the aperture is larger than the diameter of the cells to be separated, the micro-filtration membrane is made of nylon or nitrocellulose, and after the upper filtration tube is connected with the collection tube, the large-volume cell mass in the cell suspension can be separated when the cell suspension is added, so that the large-volume cell mass is filtered on the micro-filtration membrane.

Preferably, the filter tube located above can be rotated by 180 ° relative to the filter tube located below by the action of an external force. The structure can ensure that the micro-porous filtering membrane of the upper filtering pipe is aligned to the passing notch of the lower filtering pipe when cell suspension is added, thereby avoiding the cell from being blocked by the lower micro-porous filtering membrane. When taking the cells, the two passage notches can be aligned again. Or the passing notch of the upper filter tube is aligned with the passing notch of the lower filter tube when the device is installed, and the upper filter tube is detached after the cell suspension is added.

Preferably, the height of the micro-filtration membrane close to the passing notch is lower than that of the micro-filtration membrane far away from the passing notch.

Preferably, the collecting pipe and the filtering pipe are transparent structures.

The utility model has the advantages that: (1) by adding multiple times of washing and filtering, the centrifugation times and time required for separating cells are reduced, and the loss of the cells after centrifugation is reduced; (2) the separated cells are convenient to take and use through the gap, and the gas can smoothly enter the collecting pipe, so that the pouring speed is increased; (3) after the upper filtering pipe is connected with the collecting pipe, the large-volume cell mass in the cell suspension can be separated when the cell suspension is added, so that the large-volume cell mass is filtered on the micro-porous filtering membrane.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is a schematic structural view of example 2.

In the figure: the collecting pipe 1, the pipe cap 2, the filter pipe 3, the micro-porous filter membrane 3.1 and the passing notch 3.2.

Detailed Description

The invention is further described with reference to the accompanying drawings and specific embodiments.

Example 1:

as shown in figure 1, a novel cell filtration centrifuging tube that can make up, including collecting pipe 1 and pipe cap 2, be equipped with filter tube 3 between collecting pipe 1 and the pipe cap 2, the upper end threaded connection of filter tube 3 lower extreme and collecting pipe 1, filter tube 3 upper end and pipe cap 2 threaded connection are equipped with millipore filtration membrane 3.1 in the collecting pipe 1, and one side of millipore filtration membrane 3.1 is equipped with through breach 3.2. The collecting pipe 1 and the filtering pipe 3 are transparent structures.

Among the above-mentioned technical scheme, the filter tube 3 sets 1um, the microfiltration membrane 3.1 in multiple apertures such as 5um or 10um, can select the filter tube 3 in different apertures as required, microfiltration membrane 3.1 adopts nylon material or nitrocellulose material, microfiltration membrane 3.1 approximately accounts for the half of 3 body cross-sectional areas of filter tube, the remainder forms the passing through breach 3.2 of adding solution with the body inner wall, cell instrument of taking can pass and take the cell through breach 3.2. The filter pipe 3 can be connected with the pipe cap 2 through threads to form a closed container. Collecting pipe 1 can connect filter tube 3, can be through carrying out a lot of washing to the cell suspension before the centrifugation and filter, then cover pipe cap 2 and carry out centrifugal operation, pour out the supernatant after the centrifugation again, and microfiltration membrane 3.1 can avoid the cell to pour out. According to the technical scheme, the number of times of centrifugation and the time required for separating cells are reduced by increasing the number of times of washing and filtering, and the loss of the centrifuged cells is reduced. The gas can smoothly enter the collecting pipe 1 through the notch 3.2, and the pouring speed is increased.

Example 2:

as shown in fig. 2, on the basis of example 1, the number of the filter pipes 3 is 2, the 2 filter pipes 3 are stacked up and down, the 2 filter pipes 3 are detachably and hermetically connected, and the pore diameter of the microfiltration membrane 3.1 in the upper filter pipe 3 is larger than that of the microfiltration membrane 3.1 in the lower filter pipe 3. The aperture of the micro-filtration membrane 3.1 in the filter tube 3 above is 30-50um, 60-80um or 80-120um, the aperture is larger than the diameter of the cell to be separated, the micro-filtration membrane 3.1 is made of nylon material or nitrocellulose material, and after the filter tube 3 above is connected with the collecting tube 1, the large-volume cell mass in the cell suspension can be separated when the cell suspension is added, so that the large-volume cell mass is filtered on the micro-filtration membrane 3.1.

Example 3:

as shown in fig. 2, in example 2, a sealing ring is provided between the filtering tube 3 and the collecting tube 1, a sealing ring is provided between the upper end of the filtering tube 3 and the cap 2, and the height of the microfiltration membrane 3.1 near the passing notch 3.2 is lower than the height of the microfiltration membrane 3.1 far from the passing notch 3.2. The filtering pipe 3 positioned above can rotate 180 degrees relative to the filtering pipe 3 positioned below under the action of external force. The structure can ensure that the micro-porous filtering membrane 3.1 of the upper filtering tube 3 is aligned with the passing notch 3.2 of the lower filtering tube 3 when the cell suspension is added, and cells are prevented from being blocked by the micro-porous filtering membrane 3.1. When taking the cells, the two passage notches 3.2 can be aligned again. It is also possible to align the passage notch 3.2 of the upper filter tube 3 with the passage notch 3.2 of the lower filter tube 3 during the installation and to remove the upper filter tube 3 after the cell suspension has been added.

The utility model has the advantages that: (1) by adding multiple times of washing and filtering, the centrifugation times and time required for separating cells are reduced, and the loss of the cells after centrifugation is reduced; (2) the separated cells are conveniently taken and used through the gap 3.2, and gas can smoothly enter the collecting pipe 1, so that the pouring speed is increased; (3) after the upper filter tube 3 is connected to the collection tube 1, the cell suspension can be added to separate the cell mass from the cell suspension, so that the cell mass is filtered through the microfiltration membrane 3.1.

Claims

1. The utility model provides a novel cell filtration centrifuging tube that can make up, includes collecting pipe and pipe cap, characterized by, be equipped with the filter tube between collecting pipe and the pipe cap, the filter tube lower extreme is connected with the upper end of collecting pipe, and filter tube upper end and pipe cap releasable connection are equipped with millipore filtration membrane in the collecting pipe, and one side of millipore filtration membrane is equipped with through the breach.

2. The novel cell filtering centrifuge tube capable of being combined according to claim 1, wherein the lower end of the filter tube is detachably and hermetically connected with the upper end of the collecting tube, and the upper end of the filter tube is detachably and hermetically connected with the tube cap.

3. The novel combinable cell-filtering centrifuge tube of claim 1, wherein a seal ring is disposed between the filter tube and the collection tube.

4. The novel cell filtration centrifuge tube of claim 1, wherein said filter tube has a seal ring at its upper end and said cap.

5. The novel combinable cell filtering centrifuge tube according to claim 1, 2, 3 or 4, wherein the number of the filter tubes is 2, the 2 filter tubes are stacked up and down, the 2 filter tubes are connected in a detachable sealing manner, and the pore diameter of the micro-filtration membrane in the upper filter tube is larger than that of the micro-filtration membrane in the lower filter tube.

6. The novel combinable cell filtration centrifuge tube of claim 5 wherein the upper filter tube is rotatable 180 ° relative to the lower filter tube by an external force.

7. A novel cell filtration centrifuge tube assembly as claimed in claim 1, 2 or 3 wherein the height of the microfiltration membrane adjacent the gap is less than the height of the microfiltration membrane remote from the gap.

8. A novel cell-filtration centrifuge tube according to claim 1, 2 or 3 wherein said collection tube and said filtration tube are transparent.