CN111603833A - Closed autologous bone marrow mononuclear cell and platelet centrifugal filtering enricher - Google Patents
Closed autologous bone marrow mononuclear cell and platelet centrifugal filtering enricher Download PDFInfo
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- CN111603833A CN111603833A CN202010567327.2A CN202010567327A CN111603833A CN 111603833 A CN111603833 A CN 111603833A CN 202010567327 A CN202010567327 A CN 202010567327A CN 111603833 A CN111603833 A CN 111603833A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/06—Filters with filtering elements which move during the filtering operation with rotary cylindrical filtering surfaces, e.g. hollow drums
- B01D33/11—Filters with filtering elements which move during the filtering operation with rotary cylindrical filtering surfaces, e.g. hollow drums arranged for outward flow filtration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/35—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition
- B01D33/41—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition in series connection
- B01D33/42—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition in series connection concentrically or coaxially
Abstract
The invention discloses a totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator, which comprises a centrifugal tube and is characterized in that the centrifugal tube comprises a primary tube, a secondary tube and a tertiary tube; the bottom of the primary tube is provided with a primary filtering part for filtering mononuclear cells; the bottom of the secondary tube is provided with a secondary filtering part for filtering red blood cells; the bottom of the tertiary tube is a sealed bottom and is used for containing plasma and filtered platelets; the primary tube, the diode and the triode are mutually fixed, and the marrow blood sequentially passes through the primary tube and the diode and finally enters the triode; the primary filtering part at the bottom of the primary tube has a mesh pore size smaller than the minimum diameter of mononuclear cells and larger than the maximum diameter of erythrocytes; the second filtering part at the bottom of the second tube has a mesh pore size smaller than the minimum diameter of red blood cells and larger than the maximum diameter of platelets. Because a three-stage centrifugal tube is adopted, three cells are rapidly filtered and separated by utilizing centrifugal force and a filter screen.
Description
Technical Field
The invention relates to an apparatus for enriching mononuclear cells and platelets in bone marrow blood, in particular to a totally-closed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtering enricher.
Background
Bone marrow is rich in mononuclear cells, the cells have osteogenic differentiation potential and can secrete cytokines which are beneficial to bone and blood vessel regeneration, the bone marrow also contains platelets which can secrete a large amount of growth factors after being activated, a report that mononuclear cells are enriched by lymphocyte separation liquid for treating cardio-cerebral ischemia disorder diseases is provided, and a better effect is obtained. However, clinical cell biotherapy requires both high cell purity and sufficient cell number, and therefore mononuclear cells in bone marrow must be sorted and enriched, the currently most internationally applied enrichment method is lymphocyte separation liquid density centrifugation represented by Ficoll-Paque, but the method has low cell recovery efficiency, can reduce the original mononuclear cell number by 15-30% and further influences the curative effect, and the lymphocyte separation liquid is laboratory preparations at present, does not obtain clinical application permission, and has questionable biological safety; the flow cell sorting method has high accuracy, high sorting speed, high cost, difficulty in sorting a small amount of cells, difficulty in guaranteeing the aseptic requirement in the sorting process, simple and convenient operation of the immunomagnetic bead sorting method, high separation efficiency, high price, and possibility of interfering with the cell function and mechanical damage due to the combination of the antigen and the antibody on the cell membrane, and adverse effect on the cell activity due to the combination of the magnetic bead on the cell. Based on the limitations of the enrichment methods, the enrichment methods are difficult to popularize and apply in clinic, and compared with the enrichment methods, the enrichment methods design a totally-closed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator, and the enriched cells come from patients and have no immune rejection; the cells are not cultured in vitro, so that the biological risk is avoided; the enrichment process is quick and efficient, and the operation time is not delayed; the operation mode is physical centrifugation, no chemical reagent is added, and medical ethics are not violated.
In the prior art, the extraction of the mononuclear cells is mainly adsorption filtration, the filtration process is single-stage filtration or adsorption, such as the prior art CN108785779A, the filtration cassettes are the same, and the mononuclear cells are mainly adsorbed by using an adsorbing material.
For single-stage filtration, three main cells (mononuclear cells, platelets and erythrocytes) in bone marrow blood are not specifically screened in a grading way, so that the efficiency is not high, and the enriched cells are mixed more, thereby being not beneficial to the treatment effect of subsequent in vitro culture or direct injection into patients.
In addition, when carrying out centrifugal filtration, when using the test tube centrifugation, there are two kinds of modes, one is that the centrifuging tube is around axis rotation, and one is that the centrifuging tube rotates around the off-line axis. However, all the centrifuge tubes contain bone marrow plasma, and under the action of centrifugal force, cells with different densities or masses will gradually layer, as shown in fig. 1. This layering can be random. First, the boundaries between the various types of cells are not clear, the number of mononuclear cells is small, and other cells are easily mixed during extraction. Secondly, cells are not standard products, cells of the same type have different quality and different stages, red blood cells with the quality equivalent to that of mononuclear cells possibly exist, or mononuclear cells with larger quality enter the red blood cells or are left in plasma, so that the enrichment ratio of enriched mononuclear cells and platelets is low, and the difficulty of secondary extraction is high.
But the advantage of centrifugal enrichment is also obvious, namely the speed is high, and the centrifuge can rapidly separate various cells in a short time.
Disclosure of Invention
The embodiment of the application solves the technical problem which can be solved by the exclusive right in the prior art by providing the name of the invention, and realizes the technical effect.
The embodiment of the application provides a totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator, which comprises a centrifugal tube and is characterized in that the centrifugal tube comprises a primary tube, a secondary tube and a tertiary tube;
the bottom of the primary tube is provided with a primary filtering part for filtering mononuclear cells;
the bottom of the secondary tube is provided with a secondary filtering part for filtering red blood cells;
the bottom of the tertiary tube is a sealed bottom and is used for containing plasma and filtered platelets;
the primary tube, the diode and the triode are mutually fixed, and the marrow blood sequentially passes through the primary tube and the diode and finally enters the triode;
the primary filtering part at the bottom of the primary tube has a mesh pore size smaller than the minimum diameter of mononuclear cells and larger than the maximum diameter of erythrocytes;
the second filtering part at the bottom of the second tube has a mesh pore size smaller than the minimum diameter of red blood cells and larger than the maximum diameter of platelets.
Furthermore, the primary tube is provided with a primary rubber film for inserting the needle of the injector;
the secondary diode is provided with a secondary adhesive film, and the projection of the secondary adhesive film part and the projection of the primary adhesive film in the same direction are overlapped;
the three-stage tube is provided with a three-stage adhesive film, and projections of the three-stage adhesive film, the first-stage adhesive film and the second-stage adhesive film in the same direction are partially overlapped.
Preferably, the three-level adhesive film is one to three square centimeters;
the second-level adhesive film is one to three square centimeters;
the first-level adhesive film is one to three square centimeters.
Further, the primary tube is sleeved in the diode;
the diode is sleeved in the tertiary tube.
Preferably, the first and second liquid crystal materials are,
the primary tube, the diode and the triode are all straight tubes.
Furthermore, the upper side of the outer wall of the primary tube is provided with external threads, and the upper side of the inner wall of the diode is in threaded sealing connection with the outer wall of the primary tube;
the upper side of the outer wall of the diode is provided with external threads, and the upper side of the inner wall of the triode is in threaded sealing connection with the outer wall of the diode;
the top cover comprises a top cover lower thread which is in sealing connection with the thread on the inner wall of the primary pipe;
the top cover also comprises top cover upper threads, and the top cover upper threads are in threaded sealing connection with the outer wall of the tertiary pipe.
Further, the primary pipe comprises a primary straight pipe part, a primary bent pipe part, a primary positioning ring, a top cover lower thread and a top cover upper thread;
the primary bent pipe part is positioned at the lower side of the primary straight pipe part, and the included angle alpha between the axis of the primary bent pipe part and the axis of the primary straight pipe part is between 125 degrees and 160 degrees;
the volume of the first-stage straight pipe part is 1-1.5 times of that of the first-stage bent pipe part;
the top cover is in threaded sealing connection with the inner wall of the first-stage straight pipe part through the lower threads of the top cover;
the diode comprises a secondary straight pipe part, a secondary bent pipe part, a secondary positioning ring, a secondary top cover lower thread, a secondary top cover upper thread and a secondary top cover internal thread;
the second-stage bent pipe part is positioned at the lower side of the second-stage straight pipe part, and the included angle between the axis of the second-stage bent pipe part and the axis of the second-stage straight pipe part is the same as the angle alpha;
the volume of the second-stage straight pipe part is 1-1.5 times of that of the second-stage bent pipe part;
the inner thread of the secondary top cover is in threaded sealing connection with the outer wall of the primary straight pipe part;
the lower threads of the second-stage top cover are in threaded sealing connection with the inner wall of the second-stage straight pipe part;
the upper threads of the top cover are in threaded sealing connection with the inner threads of the secondary top cover;
the triode comprises a three-level straight pipe part, a three-level bent pipe part, a three-level top cover lower thread and a three-level top cover internal thread;
the three-stage bent pipe part is positioned at the lower side of the three-stage straight pipe part, and the included angle between the axis of the three-stage bent pipe part and the axis of the three-stage straight pipe part is the same as alpha;
the volume of the three-stage straight pipe part is 1-1.5 times of that of the three-stage bent pipe part;
the inner thread of the third-stage top cover is in threaded sealing connection with the outer wall of the second-stage straight pipe part; the external thread of the secondary top cover is in threaded sealing connection with the internal thread of the tertiary top cover;
the lower threads of the three-level top cover are in threaded sealing connection with the inner wall of the triode;
the first-stage straight pipe part, the second-stage straight pipe part and the third-stage straight pipe part are coaxial;
the first-stage bent pipe part, the second-stage bent pipe part and the third-stage bent pipe part are coaxial.
Furthermore, one end of the primary positioning ring is fixed on the outer side wall of the primary bent pipe part, and the other end of the primary positioning ring is fixed on the inner wall of the secondary bent pipe part;
and one end of the second-stage positioning ring is fixed with the outer wall of the second-stage bent pipe part, and the other end of the second-stage positioning ring is fixed on the inner wall of the third-stage bent pipe part.
Further, a collagen membrane is adhered to the inner side of the primary filtering part 101, and is used for adhering mononuclear cells;
the collagen membrane covers all the mesh of the filter house 101.
Furthermore, a cascade tube, a diode and a sealing ring on the top edge of the triode are fixed on the inner side of the top cover.
One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:
1. because a three-stage centrifugal tube is adopted, three cells are quickly filtered and separated by utilizing centrifugal force and a filter screen;
2. the three-stage filtration can separate three cells clearly, on one hand, all kinds of cells can be basically and completely obtained, and on the other hand, the cells are prevented from being mixed.
3. The centrifuging tube can be totally enclosed, avoids contacting with external environment, and the security is higher.
Drawings
FIG. 1 is a schematic view of a prior art centrifuge tube.
FIG. 2 is a schematic diagram of a manifold centrifuge tube configuration.
Fig. 3 is a schematic view of a bent-tube centrifuge tube structure.
Fig. 4 is a cross-sectional view of fig. 3.
Fig. 5 is a cross-sectional view of the top cover.
FIG. 6 is a cross-sectional view of the secondary overcap.
FIG. 7 is a cross-sectional view of the three-stage lid.
In the figure, a primary pipe 10, a primary filtering part 101, a primary straight pipe part 11, a primary bent pipe part 12, a primary positioning ring 13, a top cover 14, a top cover lower thread 141, a top cover upper thread 142 and a drawing and releasing pipe 15; a first-stage adhesive film 16;
the diode 20, the secondary filtering part 201, the secondary straight pipe part 21, the secondary bent pipe part 22, the secondary positioning ring 23, the secondary top cover 24, the secondary top cover lower thread 241, the secondary top cover upper thread 242, the secondary top cover internal thread 243 and the secondary adhesive film 26;
the three-stage rubber film sealing structure comprises a three-stage pipe 30, a three-stage sealing bottom 301, a three-stage straight pipe part 31, a three-stage bent pipe part 32, a three-stage positioning ring 33, a three-stage top cover 34, a three-stage top cover lower thread 341, a three-stage top cover internal thread 343 and a three-stage rubber film 36.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
This application utilizes three-stage centrifuging tube to filter screen cloth on two-stage centrifuging tube separates mononuclear cell, platelet and red blood cell. And the centrifugation mode can enrich various cells more quickly.
A totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration enricher comprises a centrifuge tube, and is characterized in that the centrifuge tube comprises a primary tube 10, a secondary tube 20 and a tertiary tube 30;
the bottom 10 of the primary tube is provided with a primary filtering part 101 for filtering mononuclear cells; the diameter of a screen hole of the first-stage filtering part 101 is 8-9 microns, (the diameter of a mononuclear cell is 10-20 microns);
the bottom 20 of the secondary tube is provided with a secondary filtering part 201 for filtering red blood cells; the second stage filter 201 has mesh diameter of 4-5 μm (erythrocyte diameter of 6-8 μm)
The bottom 30 of the tertiary tube is a sealed bottom 301 for containing plasma and filtered platelets;
the primary tube, the diode and the triode are mutually fixed, and the marrow blood sequentially passes through the primary tube 10 and the diode 20 and finally enters the triode 30;
the primary filtering part at the bottom of the primary tube has a mesh pore size smaller than the minimum diameter of mononuclear cells and larger than the maximum diameter of erythrocytes;
the second filtering part at the bottom of the second tube has a mesh pore size smaller than the minimum diameter of red blood cells and larger than the maximum diameter of platelets.
Injecting 150-200ml bone marrow blood into the tube, centrifuging by a centrifuge, injecting physiological saline into the primary tube 10, and washing the mononuclear cells out of the primary tube 10. The platelets have good improvement effect on wound healing, and can also be extracted from blood plasma. Of course, a four-stage tube can be added to filter platelets. The plasma and red blood cells may be re-infused into the patient. The whole process is closed, the pumping and discharging tube 15 can be fixedly arranged at the top of the primary tube 10 to directly receive the bone marrow and the plasma of the patient, and the whole centrifugal process is closed to avoid pollution.
Example two
The simple centrifugation often fails to satisfy the separation requirement without mixing. It is often necessary to inject an auxiliary fluid, such as saline, into each tube to flush the walls of the tube. Therefore, rubber membranes or rubber plugs are arranged on the stage pipes, so that the needle pipes can be conveniently inserted, and liquid leakage cannot be caused.
The primary tube is provided with a primary rubber film 16 for inserting the needle of the injector;
the secondary diode is provided with a secondary adhesive film 26, and the projection of the secondary adhesive film 26 and the projection of the primary adhesive film 16 in the same direction are overlapped;
the third-stage tube is provided with a third-stage adhesive film 36, and projections of the third-stage adhesive film 36, the first-stage adhesive film 16 and the second-stage adhesive film 26 in the same direction are partially overlapped.
Of course, if the tops of the centrifugal tubes at all stages are separated from each other, the top cover can be provided with an adhesive film.
When the adhesive film is arranged on the pipe wall, the size is preferably
The third-level adhesive film 36 is one to three square centimeters;
the second-level adhesive film 26 is one to three square centimeters;
the first-level adhesive film 16 is one to three square centimeters.
The shape of the plastic film is square, oval or round. Of course, if the rubber plugs are used, the rubber plugs are preferably cylindrical or conical, and the rubber plugs on the centrifuge tubes of all stages are coaxial.
EXAMPLE III
The centrifugal tubes at different levels have various positions and connection relations, and are sleeved together or share a straight tube, and the two-level or three-level filtration is realized.
When the centrifugal tubes of all levels are sleeved together, the primary tube 10 is sleeved in the diode 20; the diode 20 is sleeved in the triode 30.
Example four
The primary tube 10, the diode 20 and the triode 30 are all straight tubes;
the bottom of the tube can be flat, tapered, or spherical.
The diameter of the primary tube 10 is 1.5-3.5 cm. Based on this, the axial length of the tube is adjusted in accordance with the blood volume.
EXAMPLE five
In order to facilitate fixing and sealing and convenient disassembly after centrifugal separation, the centrifugal tubes at all levels are designed into threaded sealing connection.
The upper side of the outer wall of the primary tube 10 is provided with external threads, and the upper side of the inner wall of the secondary tube 20 is in threaded sealing connection with the outer wall of the primary tube 10;
the upper side of the outer wall of the diode 20 is provided with external threads, and the upper side of the inner wall of the triode 30 is in threaded sealing connection with the outer wall of the diode 20;
the top cover 14 comprises top cover lower threads 141 which are in threaded sealing connection with the inner wall of the primary pipe 10;
top 14 also includes top threads 142, and top threads 142 are in threaded sealing engagement with the outer wall of tertiary tube 30.
The top cap 14 functions primarily as a seal, thus sealing the nozzle at the top of all centrifuge tubes.
EXAMPLE six
The centrifugal mode is usually that the centrifuging tube rotates around the axis of non self, and the branch pipe can rotate around the pivot with certain angle of inclination, is that the centrifugal force that lets liquid receive can have more stress to point to the centrifuging tube bottom for the separation.
However, the tilt angle is limited because the volume of the centrifuge tube is limited, and the liquid with an excessive tilt angle can contaminate the top cover or more of the tube wall. The contaminated tube wall and the top cover are often only contacted with liquid when centrifugation is not started, and once the centrifugation is started, the liquid leaves, which possibly causes some cells which are not expected to be left in the primary tube to be attached to the tube wall and the top cover due to the tension of water or the adhesion force of the cell surface, so that the separation is not clear.
Therefore, the centrifugal tube is in a branch tube shape and can also be in a bent tube shape; the return bend is vertical setting in time, and the bent pipe portion of bottom still can be to liquid conduction and the parallel power of return bend axial. On the other hand, the straight pipe portion of the bent pipe is arranged obliquely, and the bent pipe portion may even be substantially parallel to the horizontal plane. This is the most efficient centrifuge and does not contaminate the top cover or more of the tube wall.
The primary pipe 10 comprises a primary straight pipe part 11, a primary bent pipe part 12, a primary positioning ring 13, a top cover 14, a top cover lower thread 141 and a top cover upper thread 142;
the primary bent pipe part 12 is positioned at the lower side of the primary straight pipe part 11, and an included angle alpha between the axis of the primary bent pipe part 12 and the axis of the primary straight pipe part 11 is between 125 degrees and 160 degrees;
the volume of the first-stage straight pipe part 11 is 1-1.5 times of the volume of the first-stage bent pipe part 12;
the top cover 14 is in threaded sealing connection with the inner wall of the first-stage straight pipe part 11 through a top cover lower thread 141;
the diode 20 comprises a secondary straight pipe part 21, a secondary bent pipe part 22, a secondary positioning ring 23, a secondary top cover 24, a secondary top cover lower thread 241, a secondary top cover upper thread 242 and a secondary top cover inner thread 243;
the secondary bent pipe part 22 is positioned at the lower side of the secondary straight pipe part 21, and the included angle between the axis of the secondary bent pipe part 22 and the axis of the secondary straight pipe part 21 is the same as the angle alpha;
the volume of the second-stage straight pipe part 21 is 1-1.5 times of that of the second-stage bent pipe part 22;
the secondary top cover internal thread 243 of the secondary top cover 24 is in threaded sealing connection with the outer wall of the primary straight pipe part 11;
the lower threads 241 of the secondary top cover are in threaded sealing connection with the inner wall of the secondary straight pipe part 21;
the top cover upper threads 142 are in threaded sealing connection with the secondary top cover internal threads 243;
the triode 30 comprises a three-stage straight pipe part 31, a three-stage bent pipe part 32, a three-stage top cover 34, a three-stage top cover lower thread 341 and a three-stage top cover internal thread 343;
the third-stage bent pipe part 32 is positioned at the lower side of the third-stage straight pipe part 31, and the included angle between the axis of the third-stage bent pipe part 32 and the axis of the third-stage straight pipe part 31 is the same as alpha;
the volume of the three-stage straight pipe part 31 is 1-1.5 times of that of the three-stage bent pipe part 32;
the third-stage top cover internal thread 343 is in threaded sealing connection with the outer wall of the second-stage straight pipe part 21; the secondary top cover external threads 241 are in threaded sealing connection with the tertiary top cover internal threads 343;
the lower thread 341 of the third-level top cover is in threaded sealing connection with the inner wall of the triode 30;
the first-stage straight pipe part 11, the second-stage straight pipe part 21 and the third-stage straight pipe part 31 are coaxial;
the primary bent pipe portion 12, the secondary bent pipe portion 22 and the tertiary bent pipe portion 32 are coaxial.
EXAMPLE seven
The bent pipe is not as good as the straight pipe, and the pipe wall of the outer layer and the pipe wall of the inner layer can be used for threaded sealing connection. Because of the bent pipe, the inner wall of the outer layer pipe wall must be larger than the diameter of the outer wall of the centrifugal pipe adjacent to the inner layer, and the threaded connection cannot be directly performed. After the internal and external screw threads of the top cover are sequentially installed, the bent pipe is likely to move under the centrifugal action, or the liquid amount in the centrifugal pipe is different under the external force action, and the screw threads of the branch pipe part can be driven to rotate. Additional fixing is therefore required.
Therefore, one end of the primary positioning ring 13 is fixed on the outer side wall of the primary elbow part 12, and the other end is fixed on the inner wall of the secondary elbow part 22;
one end of the second-stage positioning ring 23 is fixed to the outer wall of the second-stage bent pipe portion 22, and the other end of the second-stage positioning ring is fixed to the inner wall of the third-stage bent pipe portion 32.
Example eight
Mononuclear cells readily adhere to collagen membranes. Therefore, a collagen membrane for adhering mononuclear cells is adhered to the inner side of the primary filter portion 101; the collagen membrane covers all the mesh of the filter house 101. The shape of the collagen membrane is the same as that of the bottom end of the centrifugal tube.
Example nine
And the safety is improved for the convenience of sealing. A transistor 10, a diode 20 and a sealing ring on the top edge of the triode 30 are fixed on the inner side of the top cover 14.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration enricher comprises a centrifuge tube, and is characterized in that the centrifuge tube comprises a primary tube, a secondary tube and a tertiary tube;
the bottom of the primary tube is provided with a primary filtering part for filtering mononuclear cells;
the bottom of the secondary tube is provided with a secondary filtering part for filtering red blood cells;
the bottom of the tertiary tube is a sealed bottom and is used for containing plasma and filtered platelets;
the primary tube, the diode and the triode are mutually fixed, and the marrow blood sequentially passes through the primary tube and the diode and finally enters the triode;
the primary filtering part at the bottom of the primary tube has a mesh pore size smaller than the minimum diameter of mononuclear cells and larger than the maximum diameter of erythrocytes;
the second filtering part at the bottom of the second tube has a mesh pore size smaller than the minimum diameter of red blood cells and larger than the maximum diameter of platelets.
2. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to claim 1,
the primary tube is provided with a primary rubber film for inserting the needle head of the injector;
the secondary diode is provided with a secondary adhesive film, and the projection of the secondary adhesive film part and the projection of the primary adhesive film in the same direction are overlapped;
the three-stage tube is provided with a three-stage adhesive film, and projections of the three-stage adhesive film, the first-stage adhesive film and the second-stage adhesive film in the same direction are partially overlapped.
3. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to claim 2,
the third-level adhesive film is one to three square centimeters;
the second-level adhesive film is one to three square centimeters;
the first-level adhesive film is one to three square centimeters.
4. The totally-closed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to any one of claims 1 to 3, comprises a centrifuge tube, and is characterized in that,
the primary pipe is sleeved in the diode;
the diode is sleeved in the tertiary tube.
5. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to claim 4, which is characterized in that,
the primary tube, the diode and the triode are all straight tubes.
6. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to claim 5, which is characterized in that,
the upper side of the outer wall of the primary tube is provided with external threads, and the upper side of the inner wall of the diode is in threaded sealing connection with the outer wall of the primary tube;
the upper side of the outer wall of the diode is provided with external threads, and the upper side of the inner wall of the triode is in threaded sealing connection with the outer wall of the diode;
the top cover comprises a top cover lower thread which is in sealing connection with the thread on the inner wall of the primary pipe;
the top cover also comprises top cover upper threads, and the top cover upper threads are in threaded sealing connection with the outer wall of the tertiary pipe.
7. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to claim 4, which is characterized in that,
the primary pipe comprises a primary straight pipe part, a primary bent pipe part, a primary positioning ring, a top cover lower thread and a top cover upper thread;
the primary bent pipe part is positioned at the lower side of the primary straight pipe part, and the included angle alpha between the axis of the primary bent pipe part and the axis of the primary straight pipe part is between 125 degrees and 160 degrees;
the volume of the first-stage straight pipe part is 1-1.5 times of that of the first-stage bent pipe part;
the top cover is in threaded sealing connection with the inner wall of the first-stage straight pipe part through the lower threads of the top cover;
the diode comprises a secondary straight pipe part, a secondary bent pipe part, a secondary positioning ring, a secondary top cover lower thread, a secondary top cover upper thread and a secondary top cover internal thread;
the second-stage bent pipe part is positioned at the lower side of the second-stage straight pipe part, and the included angle between the axis of the second-stage bent pipe part and the axis of the second-stage straight pipe part is the same as the angle alpha;
the volume of the second-stage straight pipe part is 1-1.5 times of that of the second-stage bent pipe part;
the inner thread of the secondary top cover is in threaded sealing connection with the outer wall of the primary straight pipe part;
the lower threads of the second-stage top cover are in threaded sealing connection with the inner wall of the second-stage straight pipe part;
the upper threads of the top cover are in threaded sealing connection with the inner threads of the secondary top cover;
the triode comprises a three-level straight pipe part, a three-level bent pipe part, a three-level top cover lower thread and a three-level 30-level top cover internal thread;
the three-stage bent pipe part is positioned at the lower side of the three-stage straight pipe part, and the included angle between the axis of the three-stage bent pipe part and the axis of the three-stage straight pipe part is the same as alpha;
the volume of the three-stage straight pipe part is 1-1.5 times of that of the three-stage bent pipe part;
the inner thread of the third-stage top cover is in threaded sealing connection with the outer wall of the second-stage straight pipe part; the external thread of the secondary top cover is in threaded sealing connection with the internal thread of the tertiary top cover;
the lower threads of the three-level top cover are in threaded sealing connection with the inner wall of the triode;
the first-stage straight pipe part, the second-stage straight pipe part and the third-stage straight pipe part are coaxial;
the first-stage bent pipe part, the second-stage bent pipe part and the third-stage bent pipe part are coaxial.
8. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to claim 7,
one end of the primary positioning ring is fixed on the outer side wall of the primary bent pipe part, and the other end of the primary positioning ring is fixed on the inner wall of the secondary bent pipe part;
and one end of the second-stage positioning ring is fixed with the outer wall of the second-stage bent pipe part, and the other end of the second-stage positioning ring is fixed on the inner wall of the third-stage bent pipe part.
9. The totally-closed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to any one of claims 1 to 8,
a collagen membrane is adhered to the inner side of the primary filtering part 101 and is used for adhering mononuclear cells;
the collagen membrane covers all the mesh of the filter house 101.
10. The fully closed autologous bone marrow mononuclear cell and platelet rapid centrifugal filtration concentrator according to claim 9, wherein a sealing ring with a cascade tube, a diode and a triode top edge is fixed on the inner side of the top cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010567327.2A CN111603833A (en) | 2020-06-19 | 2020-06-19 | Closed autologous bone marrow mononuclear cell and platelet centrifugal filtering enricher |
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| US6291249B1 (en) * | 1999-03-02 | 2001-09-18 | Qualigen, Inc. | Method using an apparatus for separation of biological fluids |
| CN201337913Y (en) * | 2009-01-06 | 2009-11-04 | 李作水 | Novel test tube |
| CN202237480U (en) * | 2011-10-01 | 2012-05-30 | 别海文 | Cell washer for examination |
| CN203768278U (en) * | 2014-03-27 | 2014-08-13 | 郑州大学 | Plant intercellular fluid protein preparation and concentration composite pipe |
| CN205386371U (en) * | 2015-12-25 | 2016-07-20 | 天津锐尔康生物科技有限公司 | Unicellular quick separation device |
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