CN205386371U - Unicellular quick separation device - Google Patents
Unicellular quick separation device Download PDFInfo
- Publication number
- CN205386371U CN205386371U CN201521098018.6U CN201521098018U CN205386371U CN 205386371 U CN205386371 U CN 205386371U CN 201521098018 U CN201521098018 U CN 201521098018U CN 205386371 U CN205386371 U CN 205386371U
- Authority
- CN
- China
- Prior art keywords
- sleeve pipe
- cell
- screen cloth
- centrifuge tube
- unicellular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Centrifugal Separators (AREA)
Abstract
The utility model provides an unicellular quick separation device, including the centrifuging tube, be equipped with the sleeve pipe in the centrifuging tube, the sleeve pipe is connected for dismantling with the centrifuging tube, and the sleeve pipe upper end is equipped with the lid, lid one end and sleeve pipe fixed connection, the lid other end is connected for the buckle with the sleeve pipe opening is marginal, and the sleeve pipe bottom is equipped with the cell screen cloth, and the cell screen cloth is connected for dismantling with the sleeve pipe, and the sheathed tube bottom is equipped with the opening, and there is certain distance in the centrifuging tube bottom the sleeve pipe. The beneficial effects of the utility model are that: filter the cell that is less than screen net hole footpath through the cell screen cloth, the cell that is greater than the screen net hole footpath promptly stops on cell screen cloth upper strata, in the cell that is less than the screen net hole footpath then gets into the centrifuging tube of lower floor through the opening. Need not centrifugal operation like this, only the control through screen net hole footpath size in born of the same parents' screen cloth alright directly obtain corresponding dimensional requirement's single cell suspension. Single cell separation's step has been simplified in this design, has improved separation efficiency.
Description
Technical field
This utility model relates to biological cell separation technology field, especially relates to a kind of unicellular fast separation device.
Background technology
In biotic experiment process, it is often necessary to biological cell is easily separated, the target cell to obtain corresponding size carries out scientific research.Traditional separation method is as, in centrifuge tube, making larger-size cell be separated with smaller cell by the centrifugal action of centrifuge by cell sample.But, carrying out rough for cell can only be classified by this operation, and the target that can not obtain corresponding size is unicellular.
As can be seen here, how to work out a kind of unicellular fast separation device, there is the function of rapid screening target cell, be current those skilled in the art's problem demanding prompt solution.
Summary of the invention
In order to solve the problems referred to above, this utility model provides a kind of simple in construction and the unicellular fast separation device having good stability.
A kind of unicellular fast separation device of this utility model includes centrifuge tube, it is provided with sleeve pipe in described centrifuge tube, described sleeve pipe and described centrifuge tube are for removably connecting, the upper end of described sleeve pipe is provided with lid, one end of described lid is fixing with described sleeve pipe to be connected, the edge of opening of the other end of described lid and described sleeve pipe is for snapping connection, the bottom of described sleeve pipe is provided with cell screen cloth, described cell screen cloth and described sleeve pipe are for removably connecting, the bottom of described sleeve pipe is provided with the opening for being flowed into by cell solution in described centrifuge tube, certain distance is there is with described sleeve bottom bottom described centrifuge tube.
Further, the sieve screen apertures of described cell screen cloth is square, and the length of side of each described sieve screen apertures is 59.5-60.4 μm.
Further, being placed in described centrifuge tube in described sleeve pipe, described sleeve pipe and described centrifuge tube are for being slidably connected.
Further, described cell screen cloth overlaps mutually with the bottom of described sleeve pipe.
Further, the distance between bottom described sleeve bottom and described centrifuge tube is be more than or equal to 2cm.
A kind of unicellular fast separation device of this utility model, compared with prior art has the advantage that
First, described sleeve pipe is first placed in described centrifuge tube by this unicellular fast separation device, cell suspension will be grown again or flesh tissue lapping liquid will add in described sleeve pipe, by described cell screen cloth, the cell less than mesh size is screened, namely more than the cellular retention of the mesh size upper strata at described cell screen cloth, less than in the described centrifuge tube that the cell of mesh size then enters lower floor by described opening.So without centrifugally operated, only just can directly obtain, by the control of mesh size size in described cell screen cloth, the individual cells suspension that corresponding size requires.This design simplifies the step of unicellular separation, improves separation efficiency.
Second, the sieve screen apertures of cell screen cloth described in this unicellular fast separation device is square, and the length of side of each described sieve screen apertures is 59.5-60.4 μm.Why so design is to be sized larger than the cell of 60 μm according to experiment demand by described disposable elimination, obtaining being smaller in size than the cell suspension of 60 μm.
3rd, it is placed in described centrifuge tube in sleeve pipe described in this unicellular fast separation device, described sleeve pipe and described centrifuge tube are for being slidably connected.This design is on the one hand in order to the assembling of described sleeve pipe Yu described centrifuge tube is simpler；It is on the other hand described sleeve pipe taken out from described centrifuge tube thus from described centrifuge tube, extracting directly is out by the single cell suspension of gained in order to convenient.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation of this utility model middle sleeve.
Fig. 3 is the structural representation of cell screen cloth in this utility model.
In figure:
1, lid 2, sleeve pipe 3, cell screen cloth
4, centrifuge tube
Detailed description of the invention
In order to be better understood from this utility model, below in conjunction with specific embodiments and the drawings, this utility model is conducted further description.
As Figure 1-3, a kind of unicellular fast separation device includes centrifuge tube 4, it is provided with sleeve pipe 2 in described centrifuge tube 4, described sleeve pipe 2 and described centrifuge tube 4 are for removably connecting, the upper end of described sleeve pipe 2 is provided with lid 1, one end of described lid 1 is fixing with described sleeve pipe 2 to be connected, the edge of opening of the other end of described lid 1 and described sleeve pipe 2 is for snapping connection, the bottom of described sleeve pipe 2 is provided with cell screen cloth 3, described cell screen cloth 3 and described sleeve pipe 2 are for removably connecting, the bottom of described sleeve pipe 2 is provided with for cell solution flows into the opening in described centrifuge tube 4, certain distance is there is with bottom described sleeve pipe 2 bottom described centrifuge tube 4.
By described sleeve pipe 2 is first placed in described centrifuge tube 4 in the present embodiment, then will grow in cell suspension or the flesh tissue lapping liquid described sleeve pipe 2 of addition, by described cell screen cloth 3, the cell less than mesh size be screened.Namely more than the cellular retention of the described mesh size upper strata at described cell screen cloth 3, less than in the described centrifuge tube 4 that the cell of described screen cloth then enters lower floor by described opening.So without centrifugally operated, only just can directly obtain, by the control of mesh size size in described cell screen cloth 3, the individual cells suspension that corresponding size requires.This design simplifies the step of unicellular separation, improves separation efficiency.
The sieve screen apertures of described cell screen cloth 3 is square, and the length of side of each described sieve screen apertures is 59.5-60.4 μm.Why so design is to be sized larger than the cell of 60 μm according to experiment demand by described disposable elimination, obtaining being smaller in size than the cell suspension of 60 μm.
Being placed in described sleeve pipe 2 in described centrifuge tube 4, described sleeve pipe 2 and described centrifuge tube 4 are for being slidably connected.This design is on the one hand in order to described sleeve pipe 2 is simpler with the assembling of described centrifuge tube 4；It is on the other hand described sleeve pipe 2 taken out from described centrifuge tube 4 thus from described centrifuge tube 4, extracting directly is out by the single cell suspension of gained in order to convenient.
In order to facilitate installation and the dismounting of described cell screen cloth 3, so described cell screen cloth 3 is connected for overlap joint with the bottom design of described sleeve pipe 2.So quickly described cell screen cloth 3 can be taken out from described sleeve pipe 2, provide convenience for cleaning described cell screen cloth 3.
In order to make the single cell suspension of gained have enough accommodation spaces, so the distance between bottom described sleeve pipe 2 and bottom described centrifuge tube 4 is designed as be more than or equal to 2cm.So it is designed to the requirement meeting experiment to gained single cell suspension volume.
Above embodiment of the present utility model is described in detail, but described content has been only preferred embodiment of the present utility model, it is impossible to be considered for limiting practical range of the present utility model.All equalizations made according to this utility model scope change and improvement etc., all should still belong within this patent covering scope.
Claims (5)
1. a unicellular fast separation device, including centrifuge tube (4), it is characterized in that: in described centrifuge tube (4), be provided with sleeve pipe (2), described sleeve pipe (2) and described centrifuge tube (4) are for removably connecting, the upper end of described sleeve pipe (2) is provided with lid (1), one end of described lid (1) is fixing with described sleeve pipe (2) to be connected, the edge of opening of the other end of described lid (1) and described sleeve pipe (2) is for snapping connection, the bottom of described sleeve pipe (2) is provided with cell screen cloth (3), described cell screen cloth (3) and described sleeve pipe (2) are for removably connecting, the bottom of described sleeve pipe (2) is provided with for cell solution flows into the opening in described centrifuge tube (4), certain distance is there is in described centrifuge tube (4) bottom with described sleeve pipe (2) bottom.
2. unicellular fast separation device according to claim 1, it is characterised in that: the sieve screen apertures of described cell screen cloth (3) is square, and the length of side of each described sieve screen apertures is 59.5-60.4 μm.
3. unicellular fast separation device according to claim 1, it is characterised in that: being placed in described centrifuge tube (4) in described sleeve pipe (2), described sleeve pipe (2) and described centrifuge tube (4) are for being slidably connected.
4. unicellular fast separation device according to claim 1, it is characterised in that: the bottom of described cell screen cloth (3) and described sleeve pipe (2) overlaps mutually.
5. the unicellular fast separation device according to any one in claim 1-4, it is characterised in that: the distance between described sleeve pipe (2) bottom and described centrifuge tube (4) bottom is be more than or equal to 2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521098018.6U CN205386371U (en) | 2015-12-25 | 2015-12-25 | Unicellular quick separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521098018.6U CN205386371U (en) | 2015-12-25 | 2015-12-25 | Unicellular quick separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205386371U true CN205386371U (en) | 2016-07-20 |
Family
ID=56380992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521098018.6U Expired - Fee Related CN205386371U (en) | 2015-12-25 | 2015-12-25 | Unicellular quick separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205386371U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553962A (en) * | 2018-05-06 | 2018-09-21 | 贺琼 | A kind of separation of solid and liquid EP pipes |
| CN109762726A (en) * | 2018-12-27 | 2019-05-17 | 临海市科晶生物科技有限公司 | A kind of cell separation apparatus and its application method of simple and efficient |
| CN111073819A (en) * | 2020-01-16 | 2020-04-28 | 厦门鹭港兆康生物科技有限公司 | Plant cell screening device and method for screening synchronized plant cell lines |
| CN111603833A (en) * | 2020-06-19 | 2020-09-01 | 徐州市中心医院 | Closed autologous bone marrow mononuclear cell and platelet centrifugal filtering enricher |
-
2015
- 2015-12-25 CN CN201521098018.6U patent/CN205386371U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553962A (en) * | 2018-05-06 | 2018-09-21 | 贺琼 | A kind of separation of solid and liquid EP pipes |
| CN109762726A (en) * | 2018-12-27 | 2019-05-17 | 临海市科晶生物科技有限公司 | A kind of cell separation apparatus and its application method of simple and efficient |
| CN111073819A (en) * | 2020-01-16 | 2020-04-28 | 厦门鹭港兆康生物科技有限公司 | Plant cell screening device and method for screening synchronized plant cell lines |
| CN111603833A (en) * | 2020-06-19 | 2020-09-01 | 徐州市中心医院 | Closed autologous bone marrow mononuclear cell and platelet centrifugal filtering enricher |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170717 Address after: 102200 No. 2, building 36, Zhenxing Road, Changping District science and Technology Park, Beijing, 2, 203 Patentee after: BEIJING TDY BIOTECH CO.,LTD. Address before: 300384 the Binhai New Area of Tianjin Huayuan Industrial Park Haitai development six road 6 Haitai green industry base building I room 712 Patentee before: Tianjin REAL-AB Biological Technology Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160720 Termination date: 20211225 |