CN111821735A - Fully-closed autologous bone marrow mononuclear cell and platelet rapid circulating filtration concentrator for regenerative medicine - Google Patents
Fully-closed autologous bone marrow mononuclear cell and platelet rapid circulating filtration concentrator for regenerative medicine Download PDFInfo
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- B01D29/016—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements with corrugated, folded or wound filtering elements
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- B01D29/50—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition
- B01D29/56—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection
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Abstract
The invention discloses a totally-enclosed autologous bone marrow mononuclear cells and platelets rapid circulating filtration concentrator for regenerative medicine, which comprises a filtration component and a circulation component, wherein the filtration component is used for obtaining required cells, the circulation component is used for introducing bone marrow blood to the filtration component in a circulating manner, the filtration component comprises three stages of filter screens, the filter apertures are sequentially arranged from large to small, the aperture of the largest stage of filter screen is used for filtering mononuclear cells, the stage aperture filter screen of the middle filter screen is used for filtering red blood cells, and the smallest stage filter screen is used for filtering platelets; the circulation component circulates the bone marrow blood and sequentially passes through the middle first-stage filter screen from the largest first-stage filter screen and finally passes through the smallest first-stage filter screen. The enrichment device can effectively improve the enrichment efficiency.
Description
Technical Field
The invention relates to an apparatus for extracting autologous mononuclear cells and platelet cells, in particular to a rapid circulating filtration concentrator for autologous bone marrow mononuclear cells and platelets.
Background
Bone marrow is rich in mononuclear cells, the cells have osteogenic differentiation potential and can secrete cytokines which are beneficial to bone and blood vessel regeneration, the bone marrow also contains platelets which can secrete a large amount of growth factors after being activated, a report that mononuclear cells are enriched by lymphocyte separation liquid for treating cardio-cerebral ischemia disorder diseases is provided, and a better effect is obtained. However, clinical cell biotherapy requires both high cell purity and sufficient cell number, and therefore mononuclear cells in bone marrow must be sorted and enriched, the currently most internationally applied enrichment method is lymphocyte separation liquid density centrifugation represented by Ficoll-Paque, but the method has low cell recovery efficiency, can reduce the original mononuclear cell number by 15-30% and further influences the curative effect, and the lymphocyte separation liquid is laboratory preparations at present, does not obtain clinical application permission, and has questionable biological safety; the flow cell sorting method has high accuracy, high sorting speed, high cost, difficulty in sorting a small amount of cells, difficulty in guaranteeing the aseptic requirement in the sorting process, simple and convenient operation of the immunomagnetic bead sorting method, high separation efficiency, high price, and possibility of interfering with the cell function and mechanical damage due to the combination of the antigen and the antibody on the cell membrane, and adverse effect on the cell activity due to the combination of the magnetic bead on the cell. Based on the limitations of the enrichment methods, the enrichment methods are difficult to popularize and apply in clinic, and compared with the enrichment methods, the enrichment methods design a fully-closed autologous bone marrow mononuclear cell and platelet rapid circulating filter enricher for regenerative medicine, the enriched cells come from the patient and have no immune rejection; the cells are not cultured in vitro, so that the biological risk is avoided; the enrichment process is quick and efficient, and the operation time is not delayed; no chemical agent is added in the operation, so that the medical ethics is not violated.
In the prior art, the extraction of the mononuclear cells is mainly adsorption filtration, the filtration process is single-stage filtration or adsorption, such as the prior art CN108785779A, the filtration cassettes are the same, and the mononuclear cells are mainly adsorbed by using an adsorbing material.
For single-stage filtration, three main cells (mononuclear cells, platelets and erythrocytes) in bone marrow blood are not specifically screened in a grading way, so that the efficiency is not high, and the enriched cells are mixed more, thereby being not beneficial to the treatment effect of subsequent in vitro culture or direct injection into patients.
The adhesive material such as collagen has low use efficiency, and the use modes of the adhesive material are basically two types, namely a, the adhesive material is used as a filter material to seal a flow pipeline and bear positive pressure or negative pressure, and b, the adhesive material acts as a filling material and does not have the requirement on sealing property.
When used as a sealing material, such as the sealing material of the prior art (CN101469321B), on the one hand, the adhesion material, especially a film-like material, especially a macromolecular material such as collagen film, has a change in porosity under positive and negative pressure, which causes the partially large sized mononuclear cells to pass through the porosity, thereby reducing the collection ratio and efficiency.
When the material is used as a filling adhesive material, the requirement on the material property is high if the material does not play a role in filtering, and the material property is basically relied on for adhering the mononuclear cells. At this time, it is obvious that the flow rate of bone marrow blood and the time and material characteristics and material structure are critical to the enrichment efficiency, and since there is no ultra-high efficiency adhesive material, it is necessary to use a large amount of adhesive material to increase the contact area and probability with bone marrow blood or to extend the cycle time at a certain flow rate. The first option, on the one hand, is costly and, on the other hand, is not suitable when the adhesive material is also to be inserted into the defective bone, since the insertion of the defective bone places requirements on the structure and formation of the adhesive material; in the second option, the enrichment time is limited, and the final option is a compromise, i.e. the highest enrichment effect is achieved at a certain time.
Disclosure of Invention
The embodiment of the application provides a fully-closed autologous bone marrow mononuclear cell and platelet rapid circulating filtration concentrator for regenerative medicine, solves the problems of low mononuclear cell enrichment efficiency, low enrichment proportion and high cost in the prior art, and realizes high enrichment efficiency, high enrichment proportion, low cost and convenient subsequent operation.
The embodiment of the application provides a totally-closed autologous bone marrow mononuclear cell and platelet rapid circulating filter concentrator for regenerative medicine, which comprises a filter component and a circulating component, wherein the filter component is used for acquiring required cells, and the circulating component is used for circularly introducing bone marrow blood into the filter component,
the filter component comprises three filter screens, the filter apertures are sequentially arranged from large to small, the aperture of the largest first filter screen is used for filtering mononuclear cells, the first filter screen in the middle is used for filtering red blood cells, and the smallest first filter screen is used for filtering platelets;
the circulation component circulates the bone marrow blood and sequentially passes through the middle first-stage filter screen from the largest first-stage filter screen and finally passes through the smallest first-stage filter screen.
Further, filtering component includes casing 10, tertiary filter screen is the stereoplasm filter screen, and the diameter is the same, and the external diameter all seals fixedly with casing 10 inner wall.
Further, tertiary filter screen is open-ended cylindric, and the sealed fixed connection that can dismantle of the face of cylinder inner wall of the biggest one-level filter screen cylinder outer wall and middle one-level filter screen, the cylindrical inner wall of the smallest one-level filter screen and the face of cylinder outer wall of middle one-level filter screen seal and can dismantle fixed connection.
Furthermore, the bottom surfaces of the three-stage filter screens are all cambered surfaces protruding downwards.
5. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid cycle filter concentrator for regenerative medicine according to any one of claims 2 to 4, wherein the inside of the largest primary filter screen is covered with a collagen membrane for adsorbing mononuclear cells.
Further, the inner side of the largest first-stage filter screen is detachably fixed with a pressing net 50 in a sealing manner, the pore diameter of the pressing net is larger than that of the largest first-stage filter screen, and a collagen membrane is pressed and covered on the inner side of the largest first-stage filter screen by the bottom surface of the pressing net.
Furthermore, the inner side of the largest first-stage filter screen is covered with a collagen membrane, the periphery of the collagen membrane is attached to the filter screen without filter holes to form an attaching part, the lower side of the attaching part of the collagen membrane is a covering part, and the total area of the covering part is larger than the area of the filter screen on the lower side of the attaching part of the collagen membrane to enable the collagen membrane to form folds.
Furthermore, the folds of the collagen membrane are regular folds, the covering part of the collagen membrane is spherical, the spherical part is provided with the folds protruding towards the inner cavity, and the edges of the folds protruding towards the inner cavity form a right angle, an obtuse angle, an arc or edges formed by three straight lines;
the symmetrical surface of the fold passes through the axis of the collagen film, the horizontal section of the fold is fan-shaped, and the vertex angle of the fan-shaped is 5-35 degrees;
the number of the folds is 6-32, and the gap between the folds is preferably not more than 2 mm;
the covering net is composed of a plurality of arc-shaped strips, the arc-shaped strips can be inserted into gaps among the folds, and the covering part of the collagen membrane is covered on the filter net in a pressing mode; the number of arc-shaped strips is not more than the number of fold gaps.
Further, the liquid inlet end and the liquid discharge end of the filtering component are communicated with the circulating pump through pipelines;
the pipeline is close to filter assembly feed inlet department and is set up two three-way valves, and the branch pipe on the three-way valve of next-door neighbour filter assembly feed inlet department is used for the feed liquor, and the branch pipe of another three-way valve is used for the flowing back.
Preferably, the pipe is a hose with a clamp for blocking the flow of liquid in the pipe.
One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:
1. because the three-stage filtration is adopted, the technical problem in the prior art is effectively solved, the mononuclear cells and the platelets are further quickly enriched, the cells are not mixed, and the mononuclear cells and the platelets are beneficial to subsequent culture or are directly injected into an affected part for treatment operation.
2. Because the filter screen is covered with the collagen membrane, the adhesion effect of the collagen membrane is utilized, and the adhesion and the extraction of the mononuclear cells are accelerated.
3. Because the pressure that utilizes covers the net, can be with collagen membrane more stable location on the filter screen, the deformation diminishes, and the adhesion is all more than unfixed condition with filterable effect, and the filter time that needs can effectively reduce, and the extraction quantity of same marrow blood is more, under the condition of shortening the time, can reduce the follow-up operation degree of difficulty, reduces by environmental pollution's risk.
4. The folds, especially the regular folds, are arranged on the collagen membrane, so that the filtering and adhering effects of the collagen membrane are better, the circulation time can be further shortened, the blood volume is the same, and the number of the enriched mononuclear cells is more. The shape of the folds has larger deformability, and is favorable for being injected into the affected part for treatment as a carrier of mononuclear cells.
5. Because of adopting two three-way valves, can be more convenient carry out operations such as feed liquor and play plasma.
Drawings
FIG. 1 is a schematic view of an embodiment of the present application;
FIG. 2 is a schematic view of a second embodiment of the present application;
fig. 3 is a third schematic diagram of the present application.
Fig. 4 is a schematic view of a collagen membrane with folds.
Figure 5 is a schematic view of a pleat having a right angle inner edge.
Figure 6 is a schematic view of the inner edge of a pleat being a circular arc.
Figure 7 is a schematic view of the inner edge of a pleat at an obtuse angle.
Figure 8 is a schematic view of a three-sided inner edge of a pleat.
Figure 9 is a schematic view of the tube clamp structure.
In the figure, a housing 10, a first filter 20, a second filter 30, a third filter 40, a press-coated mesh 50, a collagen film 21, a bonding part 211 and a fold 22 are shown.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
In order to more rapidly and effectively enrich mononuclear cells and platelets, three cells in bone marrow blood are screened by utilizing different cell sizes, wherein the outer diameter of the mononuclear cells is 10-20 microns, the outer diameter of red blood cells is 6-8 microns, and the outer diameter of the platelets is 2-4 microns. The screen cloth of different apertures of design sieves three kinds of cells in grades, can be quick effectual three kinds of different cells of screening, is taking respectively. Wherein the mononuclear cells and the platelet cells can be used for the subsequent repair of the defected bone. The plasma and red blood cells may be re-infused into the patient.
Therefore, the fully-closed autologous bone marrow mononuclear cell and platelet rapid circulating filtration concentrator for regenerative medicine comprises a filtration component and a circulation component, wherein the filtration component is used for obtaining required cells, the circulation component is used for introducing bone marrow blood to the filtration component in a circulating manner, the filtration component comprises three stages of filter screens, the filter apertures are sequentially arranged from large to small, the aperture of the largest stage of filter screen is used for filtering mononuclear cells, the stage aperture filter screen of the middle filter screen is used for filtering red blood cells, and the smallest stage filter screen is used for filtering platelets;
the circulation component circulates the bone marrow blood and sequentially passes through the middle first-stage filter screen from the largest first-stage filter screen and finally passes through the smallest first-stage filter screen.
In the first embodiment, the filter assembly includes the casing 10, three level of filter screens are the stereoplasm filter screen, are the first filter screen 20 of biggest one-level respectively, the second filter screen 30 of middle one-level, and the third filter screen 40 of minimum one-level, and three filter screen diameters are the same, and the external diameter all seals fixedly with casing 10 inner wall. Wherein the first filter 20 has a mesh size of 10 microns, the second filter 30 has a mesh size of 6 microns, and the third filter has a mesh size of 2 microns.
In the second embodiment, the three-stage filter screen is a cylinder with an open top, the outer wall of the cylindrical surface of the first filter screen 20 at the largest stage is hermetically and fixedly connected with the inner wall of the cylindrical surface of the filter screen of the second filter screen 30 at the middle stage, and the inner wall of the cylindrical surface of the third filter screen 40 at the smallest stage is hermetically and fixedly connected with the outer wall of the cylindrical surface of the filter screen of the second filter screen 30 at the middle stage. The filter screens are also all hard filter screens. All the cylindrical surfaces can be connected in a sealing mode through threads, and disassembly is convenient. Of course, the modes of plugging and buckling can be adopted.
The tertiary filter screen all is located casing 10, and the opening in first filter screen 20 top seals in casing 10 top surface inboard can dismantle fixed connection, buckle or threaded connection all can. The top surface of the shell 10 is provided with a liquid inlet, and the bottom surface is provided with a liquid outlet.
In the third embodiment, the bottom surfaces of the three stages of filter screens are all cambered surfaces protruding downwards.
In the fourth embodiment, the inside of the first filter 20 of the largest order is covered with a collagen membrane 21 for adsorbing mononuclear cells. The collagen membrane 21 is preferably provided with an upwardly convex flange 211, and the outer wall of the flange 211 is attached to the inner wall of the first filter 20 to prevent bone marrow blood from flowing out of the gap between the collagen membrane 21 and the first filter 20. Of course, this approach is essentially satisfactory for achieving rapid monocyte containment. But has requirements for placing the filter screen. The filter is preferably used in a vertical state so that the bone marrow blood flows toward the mesh surface of the filter, and the bone marrow blood does not flow between the flange 211 of the collagen membrane 21 and the first filter 20 from the side under the circulation pressure.
In the fifth embodiment, the collagen membrane can stably maintain the predetermined position in order to avoid the high flow rate or the large circulation pressure. The inner side of the first filter screen 20 at the largest stage is detachably fixed with a pressing net 50 in a sealing way, and the aperture of the pressing net is 2-5 times larger than that of the first filter screen 20 at the largest stage. And the bottom surface of the filter presses and covers the collagen membrane on the inner side of the largest filter screen. At this time, the shape and size of the bottom surface of the pressing net 50 may be the same as those of the inner side of the first filter 20, so that the collagen film can be stably attached to the inner side surface of the first filter 20. The reason for this is that the collagen membrane is a macromolecular organic membrane, and if there is no limit to the deformation of the membrane under pressure, the pores of the collagen membrane become large, and it is possible to pass mononuclear cells, which reduces the enrichment efficiency. Therefore, the collagen membrane is limited by two nets, and the deformation of the collagen membrane is reduced or avoided, so that mononuclear cells are more effectively enriched and adhered to the collagen membrane.
In the sixth embodiment, the inner side of the largest filter screen is covered with a collagen film 21, the periphery of the collagen film 21 is attached to the filter screen without filter holes to form an attaching part 21, the lower side of the attaching part 21 of the collagen film is a covering part, and the total area of the covering part is larger than the area of the filter screen on the lower side of the attaching part of the collagen film, so that the collagen film is folded. This is mainly the case when the bottom surface of the filter screen is curved. The folds at this time are mainly to increase the contact area with the mononuclear cells. The area of the covering part is 1.5-3 times of the filtering area of the first filter screen. Thus, the collagen membrane is substantially free from the problem of the increase of pores due to internal tension under the cyclic pressure because there is enough collagen membrane to buffer the tension stress. Thus, the collagen membrane 21 can function more to adhere to the mononuclear cells. At the same time, the filtering function is also more effective because there is no deformation. The thickness of the collagen film is preferably selected to be 1-2 um.
The folds of the collagen film are preferably regular folds, as shown in fig. 4-5, the covering part of the collagen film is spherical, the spherical part is provided with the folds 22 protruding towards the inner cavity, and the edges of the folds 22 protruding towards the inner cavity form a right angle, an obtuse angle, an arc or edges formed by three straight lines;
the symmetrical surface of the fold passes through the axis of the collagen film, the horizontal section of the fold is fan-shaped, and the vertex angle of the fan-shaped is 5-35 degrees;
the number of the folds is 6-32, and the gap between the folds is preferably not more than 2 mm;
the overlaying net 50 is composed of a plurality of arc-shaped strips, the arc-shaped strips can be inserted into gaps among the folds, and the overlaying part of the collagen membrane is overlaid on the filter net in an overlaying mode; the number of arc-shaped strips is not more than the number of fold gaps.
Therefore, when bone marrow blood flows downwards, the folds firstly adsorb mononuclear cells, and then the mononuclear cells are filtered by the collagen membrane in the gaps of the folds and the first filter screen 20, so that the mononuclear cells can be quickly retained and adsorbed on the collagen membrane. Compared with the method of simply using a stretched or filled adsorption material, the collagen membrane can play a role in filtering and adsorbing at the same time. The collagen film has small deformation and large adsorption area, and the acquisition of the mononuclear cells is faster and the proportion is higher. In addition, the fold shape can also be used as a carrier of the nucleus cells directly during the operation, and the fold 22 is still arranged in the inner cavity by changing the overall shape of the collagen membrane, so that the operation efficiency can be further improved.
And the circulating assembly comprises a circulating pump and a pipeline, and the pipeline is communicated with the liquid inlet end and the liquid discharge end of the filtering assembly.
The liquid inlet end and the liquid discharge end of the filtering component are communicated with the circulating pump through pipelines;
the pipeline is close to filter assembly feed inlet department and is set up two three-way valves, and the branch pipe on the three-way valve of next-door neighbour filter assembly feed inlet department is used for the feed liquor, and the branch pipe of another three-way valve is used for the flowing back.
For ease of installation and use of the circulation assembly, the conduit is preferably a hose with a clamp for blocking the flow of fluid in the conduit. The circulating pump is a peristaltic pump. Peristaltic pumps can create suction pressure by squeezing a hose. The circulation speed is 10-60 ml/min.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A totally-enclosed autologous bone marrow mononuclear cells and platelets rapid circulating filter concentrator for regenerative medicine comprises a filter component and a circulating component, wherein the filter component is used for obtaining required cells, and the circulating component is used for circularly introducing bone marrow blood into the filter component,
the filter component comprises three filter screens, the filter apertures are sequentially arranged from large to small, the aperture of the largest first filter screen is used for filtering mononuclear cells, the first filter screen in the middle is used for filtering red blood cells, and the smallest first filter screen is used for filtering platelets;
the circulation component circulates the bone marrow blood and sequentially passes through the middle first-stage filter screen from the largest first-stage filter screen and finally passes through the smallest first-stage filter screen.
2. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid circulating filter concentrator for regenerative medicine according to claim 1, wherein the filter assembly comprises a casing 10, the third filter screen is a hard filter screen, and has the same diameter and the same outer diameter as the inner wall of the casing 10.
3. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid circulating filtration and enrichment device for regenerative medicine according to claim 1, characterized in that the three-stage filter screens are cylindrical with an open top end, the cylindrical outer wall of the largest first-stage filter screen is hermetically, detachably and fixedly connected with the cylindrical inner wall of the middle first-stage filter screen, and the cylindrical inner wall of the smallest first-stage filter screen is hermetically, detachably and fixedly connected with the cylindrical outer wall of the middle first-stage filter screen.
4. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid circulating filter concentrator for regenerative medicine according to claim 3, wherein the bottom surfaces of the three-stage filter screens are all cambered surfaces protruding downwards.
5. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid cycle filter concentrator for regenerative medicine according to any one of claims 2 to 4, wherein the inside of the largest primary filter screen is covered with a collagen membrane for adsorbing mononuclear cells.
6. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid circulating filtration and enrichment device for regenerative medicine according to claim 5, wherein the inside of the largest primary filter screen is detachably, hermetically and fixedly provided with a pressing and covering net 50, the pore size of the pressing and covering net is larger than that of the largest primary filter screen, and the bottom surface of the pressing and covering net presses and covers a collagen membrane on the inside of the largest primary filter screen.
7. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid cycle filter concentrator for regenerative medicine according to any one of claims 2 to 4 or 6, wherein a collagen membrane is covered on the inner side of the largest-order filter screen, the periphery of the collagen membrane is attached to the filter screen without filter holes to form an attachment part, the lower side of the attachment part of the collagen membrane is a covering part, and the total area of the covering part is larger than the area of the filter screen on the lower side of the attachment part of the collagen membrane to fold the collagen membrane.
8. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid circulation filtering and enriching device for regenerative medicine according to claim 7, wherein the folds of the collagen membrane are regular folds, the covering part of the collagen membrane is spherical, the spherical part is provided with folds protruding towards the inner cavity, and the edges of the folds protruding towards the inner cavity form a right angle, an obtuse angle, an arc or three straight lines;
the symmetrical surface of the fold passes through the axis of the collagen film, the horizontal section of the fold is fan-shaped, and the vertex angle of the fan-shaped is 5-35 degrees;
the number of the folds is 6-32, and the gap between the folds is preferably not more than 2 mm;
the covering net is composed of a plurality of arc-shaped strips, the arc-shaped strips can be inserted into gaps among the folds, and the covering part of the collagen membrane is covered on the filter net in a pressing mode; the number of arc-shaped strips is not more than the number of fold gaps.
9. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid circulating filter concentrator for regenerative medicine according to claim 1, wherein a liquid inlet end and a liquid outlet end of the filter assembly are communicated with a circulating pump through pipelines;
the pipeline is close to filter assembly feed inlet department and is set up two three-way valves, and the branch pipe on the three-way valve of next-door neighbour filter assembly feed inlet department is used for the feed liquor, and the branch pipe of another three-way valve is used for the flowing back.
10. The totally-enclosed autologous bone marrow mononuclear cell and platelet rapid circulating filter concentrator for regenerative medicine according to claim 1, wherein the tube is a flexible tube with a tube clamp for blocking the liquid flow in the tube.
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| CN202010569603.9A CN111821735A (en) | 2020-06-20 | 2020-06-20 | Fully-closed autologous bone marrow mononuclear cell and platelet rapid circulating filtration concentrator for regenerative medicine |
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