CN102260628A - Device for filtering, screening, enriching and quick-compositing stem cells - Google Patents
Device for filtering, screening, enriching and quick-compositing stem cells Download PDFInfo
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- CN102260628A CN102260628A CN2010101872146A CN201010187214A CN102260628A CN 102260628 A CN102260628 A CN 102260628A CN 2010101872146 A CN2010101872146 A CN 2010101872146A CN 201010187214 A CN201010187214 A CN 201010187214A CN 102260628 A CN102260628 A CN 102260628A
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- stem cell
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- filter cartridge
- set composite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/38—Removing constituents from donor blood and storing or returning remainder to body, e.g. for transfusion
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/02—Blood transfusion apparatus
- A61M1/0281—Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0429—Red blood cells; Erythrocytes
- A61M2202/0437—Blood stem cells
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- Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract
The invention relates to a device for filtering, screening, enriching and quick-compositing stem cells, which comprises a filter, a two-link three-way plate, an extended pipe, a blood circulation driver and porous materials. The filter comprises an outer case and a filter case. The outer case comprises an outer shell and a rear cover which are detachably jointed. The outer shell is cylindrical with a front end bottom wall and the rear cover is cylindrical with a rear end bottom wall. The centers of the bottom walls are provided with a through hole respectively. The filter case is cylindrical and provided with a filter screen-like case cover and a filter screen-like case bottom. The cavity is a stem cell screening and enriching chamber with adjustable volume and inserted in the rear part of the outer shell. The porous materials are placed in the stem cell screening and enriching chamber of the filter case and matched with the stem cell screening and enriching chamber in shape. The compositing device of the invention can obtain marrow stem cells which are highly enriched on the materials in operation without cultivating marrow stem cells in vitro.
Description
Technical field
The present invention relates to a kind of medicine equipment, be specifically related to enrichment of a kind of bone marrow stem cell filtering screening and quick set composite.
Background technology
The autologous stem cells beneficiation technologies (without vitro culture) that Recent study is found, direct Hui Zhi, not only can avoid immunological rejection, and the autologous stem cells of enrichment can utilize partial microenvironment to realize its directional induction and tissue regeneration, and the treatment to some diseases has brought hope thus.Its biggest advantage is to need not vitro culture, can carry out the same period in operation, has avoided the technical problem of complex operations and relevant ethnics Problem, and this is that traditional method can't substitute and compare.
At present by hematopoietic stem cell transplantation treatment disease in the blood system; By in-vitro screening, the common cultivation and purification technique improvement lymphocyte, take precautions against graft versus host or carry out adoptive immunotherapy hematological system tumor or solid tumor; The enrichment of mesenchymal stem cells MSCs and amplification in vitro technology promote the reparation of bone and cartilage; The transplantation treatment coronary heart disease of endothelial progenitor cell, the theory and technology of all many-sides such as disease such as dendritic cell transplantation treatment tumour, islet cell transplantation treatment type i diabetes, limbal stem cell transplantation repairing corneal, neural stem cells transplantation treatment cerebral trauma and Parkinson is all in continuous development research.This shows that the prospect of the medical use of stem cell is huge, is a revolution to the traditional treatment pattern.
Along with deepening continuously of stem-cell research, (bone marrow stem cells, BMSCs) cell therapy as seed cell receives increasing concern with bone marrow stem cell.BMSCs promotes the experimentation on animals of bone reparation to obtain comparatively sure effect, still is subject to many limitations but carry out clinical treatment.The first, when treating with autologous bone marrow blood merely, because of BMSCs amount in the unit volume marrow blood makes curative effect reduce less.The second, if adopt cells in vitro to cultivate amplification, then the patient will wait for a period of time just can return and plant operation, generally needs 3 time-of-weeks approximately, can not satisfy the needs of clinical emergency treatment or timely treatment; And vitro culture must be avoided various microbiological contamination, to having relatively high expectations of environment and equipment, has therefore increased medical expense; Vitro culture often is substratum with the foetal calf serum in addition, and this has also brought the ethics dispute of clinical cell therapy; BMSCs as if can obtain high density in art without vitro culture then might provide a kind of new approach, promotes cell therapy in Clinical Application.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, enrichment of a kind of stem cell filtering screening and quick set composite are provided, it can realize can obtaining without vitro culture the bone marrow stem cell of high density in art.
The technical scheme that realizes above-mentioned purpose is: enrichment of a kind of stem cell filtering screening and quick set composite, comprise that two threeway plates that a strainer, is connected with described strainer, the prolongation pipe, that two ends are connected with described two threeway plates and described strainer respectively are located at the blood circulation drive unit on the described prolongation pipe, and a porous material.
Described strainer comprises an overcoat and a filter cartridge, described overcoat comprises shell and the bonnet that removably is bonded together, described shell is the round shape that the front end diapire is arranged, and described bonnet is the round shape that the rear end diapire is arranged, and their diapire central authorities are respectively equipped with a through hole; Described filter cartridge is a round shape, and it has at the bottom of filter screen shape lid and the filter net box, and its inner chamber is the stem cell screening enrichment chamber of adjustable volume size, and it is plugged on the inner chamber rear portion of described shell;
Described porous material is placed in the stem cell screening enrichment chamber of described filter cartridge, the form fit in the shape of this porous material and stem cell screening enrichment chamber.
Above-mentioned stem cell filtering screening enrichment and quick set composite, wherein, described porous material is the cytoskeleton material of allograph bone, collagen, degradable artificial bone or surface modification.
Above-mentioned stem cell filtering screening enrichment and quick set composite, wherein, the adjusted volume size is come by a regulating part in the stem cell screening enrichment chamber of described filter cartridge, this regulating part is that a length is 2/3rds to 1/2nd round shape of described filter cartridge length, its outside surface is provided with negative thread, the inner peripheral surface of described filter cartridge is provided with the positive thread that the negative thread with described regulating part is screwed, so that described regulating part is bonded in the inner chamber of described filter cartridge, be positioned at the front end of described regulating part at the bottom of the described filter net box.
Above-mentioned stem cell filtering screening enrichment and quick set composite, wherein, described shell and described bonnet removably are bonded together by engagement device, and this engagement device comprises the positive thread that the negative thread with on the described shell on the inner peripheral surface that is located at the negative thread on the periphery of shell rear portion and is located at lid is screwed.
Above-mentioned stem cell filtering screening enrichment and quick set composite, wherein, the two ends of described prolongation pipe are connected with described two threeway plates with the through hole of described strainer front end by public female Luer.
Above-mentioned stem cell filtering screening enrichment and quick set composite, wherein, described blood circulation drive unit can be a peristaltic pump.
The technical scheme of stem cell filtering screening of the present invention enrichment and quick set composite, it integrates the enrichment of autologous stem cells in-vitro screening, composite device, can realize can obtaining to be compounded with in art without vitro culture the biological active materials of high density bone marrow stem cell, it has following characteristics:
(1) adopts totally-enclosed, disposable pipeline, all operate in the Operation theatre and finish, guaranteed aseptic, safety, no cross infection;
(2) stem cell filtering screening of the present invention enrichment and set composite fast, by adopting different porous materials, can from marrow blood, screen and obtain the enrichment bone marrow stem cell, simultaneously with artificial bone or other biological material compound after, return and plant the affected part, carry out the bone reparation, promote osteanagenesis; This kind method has improved the efficient of treatment, has expanded use range;
(3) adopt stem cell filtering screening of the present invention enrichment and quick set composite, need not carry out cells in vitro and cultivate, the case of emergency treatment need not to wait for can directly carry out stem cell enriched treatment;
(4) stem cell filtering screening of the present invention enrichment and fast set composite can be used for the common treatment of diseases that needs bone to repair such as orthopaedics, plastic surgery, Neurological Surgery, Oral and Maxillofacial Surgery,, nonunion damaged, backbone and joint fusion, bone cyst, Periprosthetic bone grafting, facial bones reparation etc. as bone.
Description of drawings
Fig. 1 is the front view of stem cell filtering screening of the present invention enrichment and quick set composite;
Fig. 2 is the sectional view of stem cell filtering screening of the present invention enrichment and quick set composite middle filtrator;
Fig. 3 is the working state figure of stem cell filtering screening of the present invention enrichment and quick set composite.
Embodiment
Below by embodiment particularly and in conjunction with the accompanying drawings technical scheme of the present invention is described:
See also Fig. 1, stem cell filtering screening of the present invention enrichment and quick set composite comprise a strainer 1, two threeway plates 2, prolong pipe 3, blood circulation drive unit 4 and porous material 5, and wherein, an end of two threeway plates 2 is connected with strainer 1 rear end; The length that prolongs pipe 3 can be according to the use occasion transfer; The two ends that prolong pipe are connected with the through hole of strainer 1 front end and the other end of two threeway plates 2 by public female Luer 30 respectively; Blood circulation drive unit 4 can be a peristaltic pump; Public female Luer 30 preferably can lock, and it is too high and come off to prevent to prolong pipe 3 internal pressures at peristaltic pump in the process of wriggling pressurization.
See also Fig. 2 again, strainer 1 comprises overcoat 11, a filter cartridge 12 and a regulating part 13, wherein,
Overcoat 11 comprises a shell 111 and a bonnet 112 that removably is bonded together, the material of shell 111 is PC (polycarbonate), it is the round shape that the front end diapire is arranged, and bonnet 112 is the round shape that the rear end diapire is arranged, and their diapire central authorities are respectively equipped with a through hole 1110,1120; The rear portion periphery of shell 111 is provided with negative thread, and the inner peripheral surface of bonnet 112 is provided with the positive thread that the negative thread with shell 111 is screwed;
Regulating part 13 is used for regulating the volume size in the stem cell screening enrichment chamber of filter cartridge 12, this regulating part 13 is that a length is 2/3rds to 1/2nd round shape of filter cartridge 12 length, its outside surface is provided with negative thread, the inner peripheral surface of filter cartridge 12 is provided with the positive thread that the negative thread with regulating part 120 is screwed, so that regulating part 13 is bonded in the inner chamber of filter cartridge 12,122 front ends that are positioned at regulating part 13 at the bottom of the filter net box;
Porous material 5 is as the screening enrichment media of cytoskeleton as bone marrow stem cell; Porous material 5 can be the cytoskeleton material of allograph bone, collagen, degradable artificial bone and surface modification, and the form fit in the screening enrichment chamber of its shape and filter cartridge 12 also is positioned in the enrichment chamber of filter cartridge 12;
When porous material 5 is placed in the screening enrichment chamber of filter cartridge 12,122 front and back ends that lay respectively at porous material 5 at the bottom of filter screen shape lid 121 and the filter net box, in order to fixing porous material 5, prevent moving of porous material 5, and marrow blood is passed through smoothly.
122 material is PE or nylon at the bottom of filter screen shape lid 121 and the filter net box.
In one embodiment, the rear end inside bottom wall face of bonnet 112 be provided with an external diameter and regulating part 13 the internal diameter coupling nested 1121, when bonnet 112 screwed on shell 111, nested 1121 sleeves on the bonnet 112 were on the inner cavity surface of the rear portion of regulating part 13, to stablize filter cartridge 12.
See also Fig. 3 again, the principle of work of stem cell filtering screening of the present invention enrichment and quick set composite is:
Select at the bottom of the filter screen shape lid 121 of certain pore size and the filter net box 122 as required.Screw off the bonnet 112 on the strainer 1 earlier, and unload filter cartridge 12 porous material 5 is put into screening enrichment chamber, regulating part 13 in the rotating filter box 12,122 compress porous material 5 at the bottom of can regulating the size in screening enrichment chamber and making the filter net box, in the overcoat 11 of again filter cartridge 12 being packed into then.Open a switch in the two threeway plates 2 as blood inlet 21, this blood inlet 21 connects a puncture outfit 6 to connect bags of blood, another switch is also opened as venting port 22, the marrow blood of band anti-freezing is slowly pushed prolongation pipe 3 from inlet 21, blood is along entering strainer 1 as the direction of arrow among Fig. 3 by prolonging pipe 3, strainer 1 fills with the blood inlet 21 and the venting port 22 of cutting out two threeway plates 2 behind the blood, after making the filter screen shape lid 121 of front end of blood through the screening enrichment chamber that is positioned at filter cartridge 12, in the screening enrichment chamber of strainer 1, carry out screening enrichment and the while composite biological material of stem cell by porous material 5, through tens of approximately time circulate finish screening enrichment to bone marrow stem cell after, one of them switch of opening two threeway plates 2 is as reclaiming mouth, make through the blood behind the stem cell enrichment and reclaim, open strainer 1 at last and take out stem cell enriched porous material 5 standby through reclaiming mouth.
This shows, the present invention is enrichment of stem cell filtering screening and quick set composite, can be by adopting different porous materials, screening obtains the enrichment bone marrow stem cell from marrow blood, simultaneously can be compound with biological stephanoporate material such as artificial bone, Hui Zhi can carry out the bone reparation, promote osteanagenesis in the affected part.Adopt stem cell filtering screening of the present invention enrichment and quick set composite, need not to carry out cells in vitro and cultivate, can simplify the operation of clinical cell therapy carrying out the same period on hand, safe and effective.The case of emergency treatment need not to wait for can directly carry out stem cell enriched treatment.
Technician in the art will be appreciated that, above embodiment is used for illustrating the present invention, and be not to be used as limitation of the invention, as long as in connotation scope of the present invention, all will drop in claims scope of the present invention variation, the modification of the above embodiment.
Claims (6)
1. stem cell filtering screening enrichment and set composite fast, comprise that two threeway plates that a strainer, is connected with described strainer, the prolongation pipe and that two ends are connected with described two threeway plates and described strainer respectively are located at the blood circulation drive unit on the described prolongation pipe, it is characterized in that, described set composite also comprises a porous material, described strainer comprises an overcoat and a filter cartridge
Described overcoat comprises shell and the bonnet that removably is bonded together, and described shell is the round shape that the front end diapire is arranged, and described bonnet is the round shape that the rear end diapire is arranged, and their diapire central authorities are respectively equipped with a through hole;
Described filter cartridge is a round shape, and it has at the bottom of filter screen shape lid and the filter net box, and its inner chamber is the stem cell screening enrichment chamber of adjustable volume size, and it is plugged on the inner chamber rear portion of described shell;
Described porous material is placed in the stem cell screening enrichment chamber of described filter cartridge, the form fit in the shape of this porous material and stem cell screening enrichment chamber.
2. stem cell filtering screening according to claim 1 enrichment and quick set composite is characterized in that described porous material is the cytoskeleton material of allograph bone, collagen, degradable artificial bone or surface modification.
3. stem cell filtering screening according to claim 1 enrichment and quick set composite, it is characterized in that, the adjusted volume size is come by a regulating part in the stem cell screening enrichment chamber of described filter cartridge, this regulating part is that a length is 2/3rds to 1/2nd round shape of described filter cartridge length, its outside surface is provided with negative thread, the inner peripheral surface of described filter cartridge is provided with the positive thread that the negative thread with described regulating part is screwed, so that described regulating part is bonded in the inner chamber of described filter cartridge, be positioned at the front end of described regulating part at the bottom of the described filter net box.
4. stem cell filtering screening according to claim 1 enrichment and quick set composite, it is characterized in that, described shell and described bonnet removably are bonded together by engagement device, and this engagement device comprises the positive thread that the negative thread with on the described shell on the inner peripheral surface that is located at the negative thread on the periphery of shell rear portion and is located at lid is screwed.
5. stem cell filtering screening according to claim 1 enrichment and quick set composite is characterized in that the two ends of described prolongation pipe are connected with described two threeway plates with the through hole of described strainer front end by public female Luer.
6. stem cell filtering screening according to claim 1 enrichment and quick set composite is characterized in that described blood circulation drive unit can be a peristaltic pump.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101872146A CN102260628A (en) | 2010-05-31 | 2010-05-31 | Device for filtering, screening, enriching and quick-compositing stem cells |
| PCT/CN2011/073262 WO2011150726A1 (en) | 2010-05-31 | 2011-04-25 | Stem cell filtering screening enrichment and quickly compounding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101872146A CN102260628A (en) | 2010-05-31 | 2010-05-31 | Device for filtering, screening, enriching and quick-compositing stem cells |
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| Publication Number | Publication Date |
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| CN102260628A true CN102260628A (en) | 2011-11-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101872146A Pending CN102260628A (en) | 2010-05-31 | 2010-05-31 | Device for filtering, screening, enriching and quick-compositing stem cells |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN102260628A (en) |
| WO (1) | WO2011150726A1 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251988A (en) * | 2012-02-15 | 2013-08-21 | 上海交通大学医学院附属第九人民医院 | Treatment system for in-vivo perfusion screening enrichment of stem cells |
| CN104212703A (en) * | 2014-03-17 | 2014-12-17 | 南方科技大学 | System and method for in-vitro separation of acute myeloblastic leukemia cells and hemopoietic stem cells |
| CN105950457A (en) * | 2016-03-15 | 2016-09-21 | 绍兴市人民医院 | Apparatus for enriching stem cells |
| CN108159499A (en) * | 2017-12-06 | 2018-06-15 | 广西医科大学 | A kind of plural gel and its preparation method and application |
| CN111821735A (en) * | 2020-06-20 | 2020-10-27 | 徐州市中心医院 | Fully-closed autologous bone marrow mononuclear cell and platelet rapid circulating filtration concentrator for regenerative medicine |
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| CN1713951A (en) * | 2002-06-19 | 2005-12-28 | 西北生物治疗药物公司 | Tangential flow filtration devices and methods for leukocyte enrichment |
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| CN1713951A (en) * | 2002-06-19 | 2005-12-28 | 西北生物治疗药物公司 | Tangential flow filtration devices and methods for leukocyte enrichment |
| CN1886498A (en) * | 2003-11-24 | 2006-12-27 | 西北生物治疗药物公司 | Tangential flow filtration devices and methods for stem cell enrichment |
| CN200940146Y (en) * | 2006-08-18 | 2007-08-29 | 王茜 | Sieve like large intestine cells collector |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103251988A (en) * | 2012-02-15 | 2013-08-21 | 上海交通大学医学院附属第九人民医院 | Treatment system for in-vivo perfusion screening enrichment of stem cells |
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| CN105950457A (en) * | 2016-03-15 | 2016-09-21 | 绍兴市人民医院 | Apparatus for enriching stem cells |
| CN108159499A (en) * | 2017-12-06 | 2018-06-15 | 广西医科大学 | A kind of plural gel and its preparation method and application |
| CN111821735A (en) * | 2020-06-20 | 2020-10-27 | 徐州市中心医院 | Fully-closed autologous bone marrow mononuclear cell and platelet rapid circulating filtration concentrator for regenerative medicine |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011150726A1 (en) | 2011-12-08 |
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Application publication date: 20111130 |