CN205115470U - Belt screen board separation and extraction PBMC's cell separator tube - Google Patents
Belt screen board separation and extraction PBMC's cell separator tube Download PDFInfo
- Publication number
- CN205115470U CN205115470U CN201520796640.8U CN201520796640U CN205115470U CN 205115470 U CN205115470 U CN 205115470U CN 201520796640 U CN201520796640 U CN 201520796640U CN 205115470 U CN205115470 U CN 205115470U
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- sieve plate
- separator tube
- tube
- isolation
- sieve
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Abstract
The utility model relates to a belt screen board separation and extraction PBMC's cell separator tube, the separator tube includes tube cap, body, isolation sieve and parting liquid, the coaxial thread engagement of tube cap seals up suit in the top of body, the sieve is kept apart in lower part and pipe inner wall coaxial sealing installation in the body, and equipartition interval system has the several sieve mesh on the isolation sieve, should keep apart the interior bottom encapsulation cell parting liquid of body of sieve below. The isolation sieve of this separator tube can be kept apart the blood sample of adding fast in the separator tube in the sieve top, and can prevent the parting liquid phase mixing of blood preparation and sieve below, and keep apart the sieve and still avoid the injection to place upside down at the separator tube at the parting liquid of keeping apart the sieve below effectively, under the jolt vibrations or the collision condition of transportation, the top of the isolation sieve of separator tube is arrived to " palirrhea ", guarantee that this separator tube cell parting liquid before launching is in in the separator tube of the below of isolation sieve always, thereby make the cell extraction operation more reliable, it is convenient.
Description
Technical field
The utility model belongs to medical technology class technical field of medical instruments, relates to the apparatus of cellular segregation, especially a kind of cell Separating tube with sieve plate separation and Extraction PBMC.
Background technology
Peripheral blood lymphocytes (Periphearalbloodmononuclearcell---PBMC) mainly refers to lymphocyte, is the cell mass that immunological experiment is commonly used the most.In the in vitro study of Preclinic and clinic, monocyte is a kind of cell comparatively commonly used.How from peripheral blood, to isolate the higher monocyte of purity quickly and efficiently, be the problem that medicine technology field is constantly inquired into always.The method of traditional separating monocytic cell is Ficoll density gradient separation, but the difficult point of density gradient layering is: in application of sample and centrifugal process, if operator's application of sample is too fast, centrifuge tube is uneven or the instantaneous brake under high speed rotating of whizzer, capital causes the layering of monocyte and parting liquid unintelligible, all can affect separating experiment effect.In order to solve this difficult problem, existing patent " a kind of cell Separating tube (patent publication No. is: CN204022830U) for separating of extracting PBMC " can address this problem, and this cell Separating tube is with funnel dividing plate.Because the cell Separating tube of PBMC is that need to annotate after quantitative cellular segregation liquid could medical device as a whole, medical science is provided to use.PBMC cell Separating tube with funnel dividing plate shakes in transport, jolt or in inverted situation, be infused in the cellular segregation liquid of the lower partition likely by " funnel hole " anti-first half flowing into dividing plate.Such experiment operator just can not be directly injected in separator tube needing the blood preparation be separated, must also will occurring that the separator tube of this type of situation first carries out a centrifugal process, make cellular segregation liquid whole " recovery " to the bottom of separator tube, can continue to be undertaken by operating process, not only waste a large amount of human and material resources, operate also very loaded down with trivial details, bring great inconvenience to user.
By retrieval, find patent publication us as relevant to present patent application in next chapter:
A kind of cell Separating tube (CN204022830U) for separating of extracting PBMC, relate to a kind of cell Separating tube for separating of extracting PBMC, comprise pipe lid and body, described pipe lid coaxial threaded covers the top being installed on body, middle and lower part co-axial seal in described body installs a sealing coat, described sealing coat is the funnel-form of open-topped hollow, and the tapered bottom, bottom of this sealing coat, the bottom centre of described sealing coat coaxially arranges a liquid filling hole.Blood sample, due to the design of sealing coat, can be added on the liquid level of parting liquid by this cell Separating tube rapidly, and can the mixing of anti-Hemostatic Oral Liquid layer and parting liquid, and when centrifugal, brakes without the need to closing whizzer; Directly isolated PBMC easily can be poured in new centrifuge tube after centrifugal, to save time and easy to operate, achieve at short notice convenient, fast, extract PBMC efficiently, simplify operation steps.
By contrast, there are the different of essence in present patent application and above-mentioned patent publication us.
Utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, there is provided that a kind of structure is simple, design science is reliable, after the cellular segregation liquid of annotating quantitative bottom cell Separating tube, not only convenient transportation, and it can use by direct control, has saved a large amount of human and material resources, easy to use, bring the cell Separating tube of the band sieve plate separation and Extraction PBMC of great convenience to user, and this separator tube use simple operation, more can extract PBMC fast and efficiently.
The utility model realizes by the following technical solutions:
A kind of cell Separating tube with sieve plate separation and Extraction PBMC, described separator tube comprises pipe lid, body, isolation sieve plate and cellular segregation liquid, described pipe lid coaxial threaded engages and seals is set in the top of body, bottom in described body and inboard wall of tube body co-axial seal are installed and are isolated sieve plate, on isolation sieve plate, uniform interval is shaped with several sieve aperture, the body inner bottom part encapsulate cells parting liquid below this isolation sieve plate.
And the diameter of described sieve aperture is 1 ~ 3mm.
And, the bottom conically shape of described body, described isolation sieve plate is oblate column, bottom coaxial spaced in described body is upper and lower inwardly makes pressed on ring ramp shaped projection and lower annular projection, be arranged in parallel between this pressed on ring ramp shaped projection and lower annular projection, chimeric edge section of installing isolation sieve plate between this pressed on ring ramp shaped projection and lower annular projection.
And, the width of described lower annular projection, be greater than the width, highly of pressed on ring ramp shaped projection highly respectively, the nominal diameter of described isolation sieve plate is consistent with the nominal bore diameter of cell Separating tube, the diameter of isolation sieve plate is minus tolerance, the internal diameter of cell Separating tube is plus tolerance, and the two inside and outside footpath cooperatively interacts.
And the volume of described separator tube is 50ml scale loading amount, and the 15ml place of this separator tube inwall arranges isolation sieve plate.
And the bottom of described body is arc-shaped, described isolation sieve plate is in the arc-shaped raised up.
And the volume of described separator tube is 10ml scale loading amount, and the 3ml place of this separator tube inwall arranges isolation sieve plate.
And the nominal diameter of described isolation sieve plate is consistent with the nominal bore diameter of cell Separating tube, the diameter of isolation sieve plate is plus tolerance, with separator tube internal diameter phase shrink-fit each other.
Advantage of the present utility model and positively effect are:
1, this separator tube is owing to being provided with isolation sieve plate, body is divided into by isolation sieve plate, lower two parts, parting liquid is encapsulated in the body inner bottom part below isolation sieve plate, after the filling parting liquid of separator tube, pass through centrifugally operated, due to sieve plate being covered with sieve aperture, parting liquid is all collected in the separator tube of below sieve plate by sieve aperture, namely the design of this isolation sieve plate can be isolated above sieve plate on the liquid level joining separator tube inner blood sample fast and be isolated in parting liquid, and blood preparation can be prevented and be separated liquid-phase mixing below sieve plate, and, isolation sieve plate also efficiently avoid the parting liquid be infused in below isolation sieve plate and is inverted at separator tube, under jolt vibrations or the collision situation of transport, " reflux " is to the top of the isolation sieve plate of separator tube, ensure that cellular segregation liquid is in the separator tube of the below of isolation sieve plate before this separator tube is enabled always, thus it is more reliable to make cell extraction operate, convenient, cell Separating tube transport is also more convenient.
2, this separator tube is in centrifugation blood preparation process, rapidly red corpuscle in blood sample etc. can be deposited in the below of cell Separating tube through the micro-pore of sieve plate, centrifugal rear required most PBMC cells then separated liquid and isolation sieve plate are retained in the separator tube above isolation sieve plate, directly isolated PBMC can be poured in new container fast, like a cork after centrifugal, take out light, easy to operate, quick, also save time, improve the quality of isolated cell, simplify operation steps, extract PBMC efficiently.
3, the bottom of the body of this separator tube conically shape, reaction solution is mixed to get more abundant, even, the setting of pressed on ring ramp shaped projection and lower annular projection, can make to isolate sieve plate accurately can locate and can prevent from isolating the bottom that sieve plate enters body, thus lose its effect, ensure that the stability of the structure of this separator tube, also extend the work-ing life of this separator tube simultaneously.
4, the structure of this separator tube is more accurate, and separation and Extraction is more accurate.
Accompanying drawing explanation
Fig. 1 is a kind of anatomical connectivity schematic diagram of the present utility model;
Fig. 2 be isolate sieve plate in Fig. 1 overlook enlarged view;
Fig. 3 is the A bilge construction enlarged diagram in Fig. 1;
Fig. 4 is another kind of anatomical connectivity schematic diagram of the present utility model;
Fig. 5 be isolate sieve plate in Fig. 4 overlook enlarged view.
Embodiment
For content of the present utility model, Characteristic can be understood further, hereby exemplify following examples, and coordinate accompanying drawing to be described in detail as follows.It should be noted that, the present embodiment is descriptive, is not determinate, can not limit protection domain of the present utility model thus.
A kind of cell Separating tube with sieve plate separation and Extraction PBMC, as shown in Figure 1 and Figure 4, described separator tube comprises pipe lid 1, body 2, isolation sieve plate 4 and cellular segregation liquid 6, described pipe lid coaxial threaded engages and seals is set in the top of body, bottom in described body and inboard wall of tube body co-axial seal are installed and are isolated sieve plate, on isolation sieve plate, uniform interval is shaped with several sieve aperture 8 (such as small sieve aperture, its diameter range is 1 ~ 3mm, be such as 2mm), body inner bottom part encapsulate cells parting liquid below this isolation sieve plate, after the filling quantify cellular parting liquid of separator tube, pass through centrifugally operated, cellular segregation liquid is all collected in the separation tube chamber of below isolation sieve plate by sieve aperture, the extraction PBMC cell Separating tube now pre-installing cellular segregation liquid medical device can be supplied to demanding party as a whole.
This separator tube is owing to being provided with isolation sieve plate, body is divided into by isolation sieve plate, lower two parts, cellular segregation fluid-tight is contained in the body inner bottom part below isolation sieve plate, after the filling parting liquid of separator tube, pass through centrifugally operated, due to sieve plate being covered with sieve aperture, parting liquid is all collected in the separator tube of below sieve plate by sieve aperture, namely the design of this isolation sieve plate can be isolated above sieve plate on the liquid level joining separator tube inner blood sample fast and be isolated in parting liquid, and blood preparation can be prevented and be separated liquid-phase mixing below sieve plate, and, isolation sieve plate also efficiently avoid the parting liquid be infused in below isolation sieve plate and is inverted at separator tube, under jolt vibrations or the collision situation of transport, " reflux " is to the top of the isolation sieve plate of separator tube, ensure that cellular segregation liquid is in the separator tube below isolation sieve plate always before this separator tube is enabled, thus it is more reliable to make cell extraction operate, convenient, cell Separating tube transport is also more convenient.
This separator tube is in centrifugation blood preparation process, rapidly red corpuscle in blood sample etc. can be deposited in the below of cell Separating tube through the hole of sieve plate, centrifugal rear required most PBMC cells then separated liquid and isolation sieve plate are retained in the separator tube above isolation sieve plate, directly isolated PBMC can be poured in new container fast, like a cork after centrifugal, take out light, easy to operate, quick, also save time, improve the quality of isolated cell, simplify operation steps, extract PBMC efficiently.
In the present embodiment, as shown in Figure 1, Figure 2 and Figure 3, the bottom 7 conically shape of described body, reaction solution is mixed to get more abundant, even, described isolation sieve plate is oblate column, bottom coaxial spaced in described body is upper and lower inwardly makes pressed on ring ramp shaped protruding 3 and lower annular projection 5, be arranged in parallel between this pressed on ring ramp shaped projection and lower annular projection, chimeric edge section of installing isolation sieve plate between this pressed on ring ramp shaped projection and lower annular projection.The setting of this pressed on ring ramp shaped projection and lower annular projection, can make to isolate sieve plate accurately can locate and can prevent from isolating the bottom that sieve plate enters body, thus lose its effect, ensure that the stability of the structure of this separator tube, also extend the work-ing life of this separator tube simultaneously.
The width of described lower annular projection, be greater than the width, highly of pressed on ring ramp shaped projection highly respectively, the nominal diameter of described isolation sieve plate is consistent with the nominal bore diameter of cell Separating tube, the diameter of isolation sieve plate is minus tolerance, the internal diameter of cell Separating tube is plus tolerance, the two inside and outside footpath cooperatively interacts, make this separator tube structure more accurate, more accurately; The volume of described separator tube can be 50ml scale loading amount, and the 15ml place of this separator tube inwall arranges isolation sieve plate.
In the present embodiment, as shown in Figure 4 and Figure 5, the bottom of described body is arc-shaped, and described isolation sieve plate is in the arc-shaped raised up, and the inner wall lower of body closely cooperates mutually with isolation sieve plate, makes to enable in the pipe of front parting liquid below isolation sieve plate.
The volume of described separator tube can be 10ml scale loading amount, and the 3ml place of this separator tube inwall arranges isolation sieve plate; The nominal diameter of described isolation sieve plate is consistent with the nominal bore diameter of cell Separating tube, and the diameter of isolation sieve plate is plus tolerance, and with separator tube internal diameter phase shrink-fit each other, make this separator tube structure more accurate, separation and Extraction is more accurate.
The using method of the cell Separating tube of this band sieve plate separation and Extraction PBMC is as follows:
Due to band sieve plate separation and Extraction PBMC cell Separating tube inoculated install Quantitative Separation liquid, after twisting lower tube cap, directly injected in separator tube by the inboard wall of tube body of blood preparation above isolation sieve plate, lid upper tube cap, puts into whizzer this centrifuge tube and is separated with the centrifugal force of 1200*g; After separation, cell Separating tube internal cause density difference presents liquid layered, red corpuscle is at the lowest layer, up the blood plasma of granulocyte, parting liquid, mononuclearcell (tunica albuginea layer), dilution successively, wherein tunica albuginea layer is exactly the monocyte needing to extract, and directly can be poured into easily in new centrifuge tube by isolated PBMC, clean secondary again with diluent after centrifugal, the required PBMC extracted just is deposited in the bottommost of test tube, takes out.
Claims (8)
1. the cell Separating tube with sieve plate separation and Extraction PBMC, it is characterized in that: described separator tube comprises pipe lid, body, isolation sieve plate and cellular segregation liquid, described pipe lid coaxial threaded engages and seals is set in the top of body, bottom in described body and inboard wall of tube body co-axial seal are installed and are isolated sieve plate, on isolation sieve plate, uniform interval is shaped with several sieve aperture, the body inner bottom part encapsulate cells parting liquid below this isolation sieve plate.
2. the cell Separating tube of band sieve plate separation and Extraction PBMC according to claim 1, is characterized in that: the diameter of described sieve aperture is 1 ~ 3mm.
3. the cell Separating tube of band sieve plate separation and Extraction PBMC according to claim 1, it is characterized in that: the bottom conically shape of described body, described isolation sieve plate is oblate column, bottom coaxial spaced in described body is upper and lower inwardly makes pressed on ring ramp shaped projection and lower annular projection, be arranged in parallel between this pressed on ring ramp shaped projection and lower annular projection, chimeric edge section of installing isolation sieve plate between this pressed on ring ramp shaped projection and lower annular projection.
4. the cell Separating tube of band sieve plate separation and Extraction PBMC according to claim 3, it is characterized in that: the width of described lower annular projection, be greater than the width, highly of pressed on ring ramp shaped projection highly respectively, the nominal diameter of described isolation sieve plate is consistent with the nominal bore diameter of cell Separating tube, the diameter of isolation sieve plate is minus tolerance, the internal diameter of cell Separating tube is plus tolerance, and the two inside and outside footpath cooperatively interacts.
5. the cell Separating tube of the band sieve plate separation and Extraction PBMC according to claim 3 or 4, is characterized in that: the volume of described separator tube is 50ml scale loading amount, and the 15ml place of this separator tube inwall arranges isolation sieve plate.
6. the cell Separating tube of the band sieve plate separation and Extraction PBMC according to any one of Claims 1-4, is characterized in that: the bottom of described body is arc-shaped, and described isolation sieve plate is in the arc-shaped raised up.
7. the cell Separating tube of band sieve plate separation and Extraction PBMC according to claim 6, is characterized in that: the volume of described separator tube is 10ml scale loading amount, and the 3ml place of this separator tube inwall arranges isolation sieve plate.
8. the cell Separating tube of band sieve plate separation and Extraction PBMC according to claim 6, it is characterized in that: the nominal diameter of described isolation sieve plate is consistent with the nominal bore diameter of cell Separating tube, the diameter of isolation sieve plate is plus tolerance, with separator tube internal diameter phase shrink-fit each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520796640.8U CN205115470U (en) | 2015-10-15 | 2015-10-15 | Belt screen board separation and extraction PBMC's cell separator tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520796640.8U CN205115470U (en) | 2015-10-15 | 2015-10-15 | Belt screen board separation and extraction PBMC's cell separator tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205115470U true CN205115470U (en) | 2016-03-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520796640.8U Withdrawn - After Issue CN205115470U (en) | 2015-10-15 | 2015-10-15 | Belt screen board separation and extraction PBMC's cell separator tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205115470U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132278A (en) * | 2015-10-15 | 2015-12-09 | 任峰 | Cell separation pipe with screening plate for separation extracting of PBMCs |
-
2015
- 2015-10-15 CN CN201520796640.8U patent/CN205115470U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132278A (en) * | 2015-10-15 | 2015-12-09 | 任峰 | Cell separation pipe with screening plate for separation extracting of PBMCs |
| CN105132278B (en) * | 2015-10-15 | 2017-12-05 | 斯坦姆（天津）细胞工程技术有限公司 | Cell Separating tube with sieve plate separation and Extraction PBMC |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20160330 Effective date of abandoning: 20171205 |