CN106269302A - Cell centrifugation extraction machine is used in a kind of experiment - Google Patents
Cell centrifugation extraction machine is used in a kind of experiment Download PDFInfo
- Publication number
- CN106269302A CN106269302A CN201610643707.3A CN201610643707A CN106269302A CN 106269302 A CN106269302 A CN 106269302A CN 201610643707 A CN201610643707 A CN 201610643707A CN 106269302 A CN106269302 A CN 106269302A
- Authority
- CN
- China
- Prior art keywords
- test tube
- memotron
- rotating cylinder
- mounting disc
- sealing member
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 24
- 238000005119 centrifugation Methods 0.000 title claims abstract description 19
- 238000002474 experimental method Methods 0.000 title claims abstract description 17
- 238000012360 testing method Methods 0.000 claims abstract description 75
- 238000007789 sealing Methods 0.000 claims abstract description 31
- 230000000694 effects Effects 0.000 abstract description 5
- 238000011065 in-situ storage Methods 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- 238000005457 optimization Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
Abstract
The invention discloses a kind of experiment cell centrifugation extraction machine, rotating cylinder is vertically arranged in body, rotating cylinder is provided with the multiple test tubes being positioned on same circumference and accommodates chamber, mounting disc is horizontally set on above rotating cylinder and is coaxially disposed with rotating cylinder, mounting disc periphery is provided with multiple test tube sealing member, the memotron of withdrawal device is arranged on a test tube sealing member, and extraction end connects with test tube, plunger at one end is in memotron is stretched in memotron upper end and the other end is fixed with mounting disc, and drive mechanism and memotron are connected to drive memotron to move along plunger direction.The experiment cell centrifugation extraction machine designed by above-mentioned optimization, reasonable in design, on the one hand, by the test tube sealing member in mounting disc, the opening of test tube is sealed, it is ensured that time centrifugal, test tube does not shakes, on the other hand, memotron stretches in test tube after centrifugal, realize in situ extraction to separate, mixing again to layer material when preventing test tube from taking out, be greatly improved separating effect.
Description
Technical field
The present invention relates to biological cell assays technical field, particularly relate to a kind of experiment cell centrifugation extraction machine.
Background technology
Centrifuge is the separate apparatus that biology laboratory is basic, is usually used in the solid phase in separation gel liquid solution and liquid phase, day
When often testing, circumferencial direction uniform force during in order to ensure centrifugal, need manually to be placed and taken out one by one by test tube, largely effect on
Centrifugal efficiency.The liquid simultaneously taken out needs to separate one by one, on the one hand affects work efficiency, on the other hand, after separation
After liquid places a period of time, it may occur that melt the most mutually, affect centrifugal effect.
Summary of the invention
For solving technical problem present in background technology, the present invention proposes a kind of experiment cell centrifugation extraction machine.
Cell centrifugation extraction machine is used in a kind of experiment that the present invention proposes, including: body, rotating cylinder, multiple withdrawal device, installation
Dish, driving means；
Being provided with centrifugal chamber in body, rotating cylinder is vertically arranged in centrifugal chamber, and driving means and rotating cylinder are connected to drive and turn
Cylinder rotates, and rotating cylinder is provided with multiple test tube and accommodates chamber, and described test tube accommodates chamber from cylinder sidewall to away from tube rotaring axis direction
Downwardly extending, multiple test tubes accommodate chamber and are positioned on the same circumference of tube rotaring axis, and cylinder bottom is provided with the first connector；
Mounting disc is horizontally set on above rotating cylinder and is coaxially disposed with rotating cylinder, and mounting disc periphery is provided with multiple test tube and seals
Part, each test tube sealing member and a test tube accommodate chamber cooperation and are used for sealing test tube opening, set in the middle part of each test tube sealing member
There is through hole, be provided with the second connector, the second connector and the first connector bottom mounting disc and connect；
Withdrawal device includes memotron, plunger, drive mechanism, and memotron is arranged on through hole and the edge of a test tube sealing member
Test tube accommodates cavity direction and arranges, between memotron and mounting disc axis, institute is a in angle, and described test tube accommodates chamber and tube rotaring axis
Between institute be b in angle, a=b, plunger extends along memotron direction, plunger at one end in memotron is stretched in memotron upper end and with
Memotron inner wall sealing coordinates, and plunger is fixed with mounting disc away from memotron one end, and drive mechanism and memotron are connected to drive
Dynamic memotron moves along plunger direction.
Preferably, multiple test tube sealing members are distributed along mounting disc even circumferential.
Preferably, memotron is removably mounted at the through hole of test tube sealing member.
Preferably, being provided with the connecting rod arranged along its axis vertical bottom mounting disc, connecting rod top is fixing with mounting disc to be connected,
Second connector is arranged on connecting rod bottom.
In the present invention, the experiment cell centrifugation extraction machine proposed, rotating cylinder is vertically arranged in body, and rotating cylinder is provided with
The multiple test tubes being positioned on same circumference accommodate chamber, and mounting disc is horizontally set on above rotating cylinder and is coaxially disposed with rotating cylinder, install
Dish periphery is provided with multiple test tube sealing member, and the memotron of withdrawal device is arranged on a test tube sealing member, and extracts end and test tube
Connection, plunger at one end is in memotron is stretched in memotron upper end and the other end is fixed with mounting disc, and drive mechanism is with memotron even
Connect for driving memotron to move along plunger direction.The experiment cell centrifugation extraction machine designed by above-mentioned optimization, structure sets
Meter is rationally, on the one hand, sealed by the opening of test tube by the test tube sealing member in mounting disc, it is ensured that time centrifugal, test tube does not occurs
Rocking, on the other hand, memotron stretches in test tube after centrifugal, it is achieved extraction separates, to layering when preventing test tube from taking out in situ
The mixing again of material, is greatly improved separating effect.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation testing use cell centrifugation extraction machine that the present invention proposes.
Detailed description of the invention
As it is shown in figure 1, Fig. 1 is a kind of structural representation testing use cell centrifugation extraction machine that the present invention proposes.
With reference to Fig. 1, cell centrifugation extraction machine is used in a kind of experiment that the present invention proposes, and including: body 1, rotating cylinder 2, multiple takes out
Take device, mounting disc 4, driving means；
Being provided with centrifugal chamber in body 1, rotating cylinder 2 is vertically arranged in centrifugal chamber, and driving means and rotating cylinder 2 are connected to drive
Rotating cylinder 2 rotates, and rotating cylinder 2 is provided with multiple test tube and accommodates chamber, and described test tube accommodates chamber from rotating cylinder 2 sidewall to away from rotating cylinder 2 axis
Downwardly extending on direction, multiple test tubes accommodate chamber and are positioned on the same circumference of rotating cylinder 2 axis, are provided with the first connection bottom rotating cylinder 2
Part；
Mounting disc 4 is horizontally set on above rotating cylinder 2 and is coaxially disposed with rotating cylinder 2, and it is close that mounting disc 4 periphery is provided with multiple test tube
Sealing 41, each test tube sealing member 41 accommodates chamber and coordinates and be used for sealing test tube opening with test tube, each test tube sealing member
It is provided with through hole in the middle part of in the of 41, is provided with the second connector, the second connector and the first connector bottom mounting disc 4 and connects；
Withdrawal device includes memotron 31, plunger 32, drive mechanism, and memotron 31 is arranged on the logical of a test tube sealing member 41
At hole and accommodating cavity direction along test tube to arrange, between memotron 31 and mounting disc 4 axis, institute is a in angle, and described test tube accommodates
Between chamber and rotating cylinder 2 axis, institute is b in angle, a=b, and plunger 32 extends along memotron 31 direction, and plunger 32 one end is from memotron 31
In memotron 31 is stretched in upper end and coordinating with memotron 31 inner wall sealing, plunger 32 is solid with mounting disc 4 away from memotron 31 one end
Fixed, drive mechanism and memotron 31 are connected to drive memotron 31 to move along plunger 32 direction.
During the specific works of cell centrifugation extraction machine is used in the experiment of the present embodiment, first will fill liquid to be separated
Test tube is placed on the test tube of rotating cylinder and accommodates intracavity, mounting disc is arranged on rotating cylinder so that the opening of each test tube is by test tube
Sealing member seals, and the memotron of withdrawal device is positioned at ullage, along with the rotation of rotating cylinder, it is achieved centrifugal point of examination liquid in pipe
From；After Li Xin, drive mechanism drives memotron to stretch into below liquid level, and now plunger produces negative pressure by test tube point in memotron
In upper strata material suction memotron after from, thus in realizing test tube, liquid in-situ separates.
In the present embodiment, the experiment cell centrifugation extraction machine proposed, rotating cylinder is vertically arranged in body, on rotating cylinder
Being provided with the multiple test tubes being positioned on same circumference and accommodate chamber, mounting disc is horizontally set on above rotating cylinder and is coaxially disposed with rotating cylinder,
Mounting disc periphery is provided with multiple test tube sealing member, and the memotron of withdrawal device is arranged on a test tube sealing member, and extraction end with
Test tube connects, and plunger at one end is in memotron is stretched in memotron upper end and the other end is fixed with mounting disc, drive mechanism and storage
Pipe is connected to drive memotron to move along plunger direction.The experiment cell centrifugation extraction machine designed by above-mentioned optimization, knot
Structure is reasonable in design, on the one hand, sealed by the opening of test tube by the test tube sealing member in mounting disc, it is ensured that time centrifugal, test tube is not
Shaking, on the other hand, memotron stretches in test tube after centrifugal, it is achieved extraction separates in situ, when preventing test tube from taking out pair
The mixing again of layer material, is greatly improved separating effect.
In a specific embodiment, multiple test tube sealing members 41 are distributed along mounting disc 4 even circumferential.
In other detailed description of the invention, memotron 31 is removably mounted at the through hole of test tube sealing member 41.
In other detailed description of the invention, test tube stability during in order to improve centrifugal further, it is provided with edge bottom mounting disc 4
The connecting rod 42 that its axis vertical is arranged, connecting rod 42 top is fixing with mounting disc 4 to be connected, and the second connector is arranged on connecting rod 42 end
End.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (4)
1. cell centrifugation extraction machine is used in an experiment, it is characterised in that including: body (1), rotating cylinder (2), multiple withdrawal device, peace
Sabot (4), driving means；
Being provided with centrifugal chamber in body (1), rotating cylinder (2) is vertically arranged in centrifugal chamber, and driving means is connected to drive with rotating cylinder (2)
Dynamic rotating cylinder (2) rotates, and rotating cylinder (2) is provided with multiple test tube and accommodates chamber, and described test tube accommodates chamber from rotating cylinder (2) sidewall to away from turning
Downwardly extending on cylinder (2) axis direction, multiple test tubes accommodate chamber and are positioned on the same circumference of rotating cylinder (2) axis, rotating cylinder (2) bottom
It is provided with the first connector；
Mounting disc (4) is horizontally set on rotating cylinder (2) top and is coaxially disposed with rotating cylinder (2), and mounting disc (4) periphery is provided with multiple examination
Seals (41), each test tube sealing member (41) accommodates chamber and coordinates and be used for sealing test tube opening, Mei Geshi with test tube
Seals (41) middle part is provided with through hole, and mounting disc (4) bottom is provided with the second connector, the second connector and the first connector even
Connect；
Withdrawal device includes memotron (31), plunger (32), drive mechanism, and memotron (31) is arranged on a test tube sealing member (41)
Through hole and accommodate cavity direction along test tube and arrange, between memotron (31) and mounting disc (4) axis be a in angle, described
It is b in angle that test tube accommodates institute between chamber and rotating cylinder (2) axis, a=b, and plunger (32) extends along memotron (31) direction, plunger
(32) one end is stretched in memotron (31) from memotron (31) upper end and coordinates with memotron (31) inner wall sealing, and plunger (32) is remote
Fixing with mounting disc (4) from memotron (31) one end, drive mechanism and memotron (31) are connected to drive memotron (31) edge
Plunger (32) direction is moved.
Experiment cell centrifugation extraction machine the most according to claim 1, it is characterised in that multiple test tube sealing member (41) edges
Mounting disc (4) even circumferential is distributed.
Experiment cell centrifugation extraction machine the most according to claim 1, it is characterised in that memotron (31) is detachably installed
Through hole in test tube sealing member (41).
Experiment cell centrifugation extraction machine the most according to claim 1, it is characterised in that mounting disc (4) bottom is provided with edge
The connecting rod (42) that its axis vertical is arranged, connecting rod (42) top is fixing with mounting disc (4) to be connected, and the second connector is arranged on connecting rod
(42) bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610643707.3A CN106269302B (en) | 2016-08-08 | 2016-08-08 | A kind of experiment cell centrifugation extraction machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610643707.3A CN106269302B (en) | 2016-08-08 | 2016-08-08 | A kind of experiment cell centrifugation extraction machine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106269302A true CN106269302A (en) | 2017-01-04 |
| CN106269302B CN106269302B (en) | 2018-10-12 |
Family
ID=57666665
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610643707.3A Active CN106269302B (en) | 2016-08-08 | 2016-08-08 | A kind of experiment cell centrifugation extraction machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106269302B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110102413A (en) * | 2019-05-15 | 2019-08-09 | 林伟阳 | A kind of continuous centrifugal machine for cell separation |
| CN110813557A (en) * | 2019-11-25 | 2020-02-21 | 安徽科门生物科技有限公司 | Cell centrifuge |
| CN112973975A (en) * | 2021-02-24 | 2021-06-18 | 安徽中科中佳科学仪器有限公司 | High-speed centrifuge is used in laboratory of self-cleaning filtration separation |
Citations (12)
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|---|---|---|---|---|
| DE2939099A1 (en) * | 1979-09-27 | 1981-04-09 | Heraeus-Christ Gmbh, 3360 Osterode | Sample tube used in centrifuges, esp. ultracentrifuges - where tube is easily sealed by metal cap consisting of two parts joined by O=ring seal |
| DE3703514A1 (en) * | 1987-02-05 | 1988-08-18 | Hettich Andreas Fa | ANGLE HEAD FOR CENTRIFUGES |
| US5562583A (en) * | 1995-09-07 | 1996-10-08 | E. I. Du Pont De Nemours And Company | Tube adapter for centrifuge shell type rotor |
| EP1084772A1 (en) * | 1999-09-17 | 2001-03-21 | Fresenius HemoCare GmbH | Magnetic gearing and centrifuge with a magnetic gearing |
| US20040023778A1 (en) * | 2002-07-31 | 2004-02-05 | Hitachi Koki Co., Ltd. | Rotor driving apparatus |
| CN2656015Y (en) * | 2003-09-17 | 2004-11-17 | 聂建堂 | Medical centrifugal machine |
| CN201625588U (en) * | 2010-04-12 | 2010-11-10 | 瑞基海洋生物科技股份有限公司 | Centrifugal machine capable of being used for test tubes with different tube diameters |
| CN202366795U (en) * | 2011-12-26 | 2012-08-08 | 天津市东丽区疾病预防控制中心 | Fat centrifuge |
| CN203209191U (en) * | 2013-04-19 | 2013-09-25 | 应城市新都化工有限责任公司 | Test tube centrifuge |
| CN203355899U (en) * | 2013-07-25 | 2013-12-25 | 中山市生科试剂仪器有限公司 | Rotary table for test tubes of centrifugal machine |
| CN203664026U (en) * | 2014-01-17 | 2014-06-25 | 台州博曼克科学仪器有限公司 | High-speed centrifugal machine |
| CN203711166U (en) * | 2014-03-12 | 2014-07-16 | 刘树生 | Medical centrifuge |
-
2016
- 2016-08-08 CN CN201610643707.3A patent/CN106269302B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2939099A1 (en) * | 1979-09-27 | 1981-04-09 | Heraeus-Christ Gmbh, 3360 Osterode | Sample tube used in centrifuges, esp. ultracentrifuges - where tube is easily sealed by metal cap consisting of two parts joined by O=ring seal |
| DE3703514A1 (en) * | 1987-02-05 | 1988-08-18 | Hettich Andreas Fa | ANGLE HEAD FOR CENTRIFUGES |
| US5562583A (en) * | 1995-09-07 | 1996-10-08 | E. I. Du Pont De Nemours And Company | Tube adapter for centrifuge shell type rotor |
| EP1084772A1 (en) * | 1999-09-17 | 2001-03-21 | Fresenius HemoCare GmbH | Magnetic gearing and centrifuge with a magnetic gearing |
| US20040023778A1 (en) * | 2002-07-31 | 2004-02-05 | Hitachi Koki Co., Ltd. | Rotor driving apparatus |
| CN2656015Y (en) * | 2003-09-17 | 2004-11-17 | 聂建堂 | Medical centrifugal machine |
| CN201625588U (en) * | 2010-04-12 | 2010-11-10 | 瑞基海洋生物科技股份有限公司 | Centrifugal machine capable of being used for test tubes with different tube diameters |
| CN202366795U (en) * | 2011-12-26 | 2012-08-08 | 天津市东丽区疾病预防控制中心 | Fat centrifuge |
| CN203209191U (en) * | 2013-04-19 | 2013-09-25 | 应城市新都化工有限责任公司 | Test tube centrifuge |
| CN203355899U (en) * | 2013-07-25 | 2013-12-25 | 中山市生科试剂仪器有限公司 | Rotary table for test tubes of centrifugal machine |
| CN203664026U (en) * | 2014-01-17 | 2014-06-25 | 台州博曼克科学仪器有限公司 | High-speed centrifugal machine |
| CN203711166U (en) * | 2014-03-12 | 2014-07-16 | 刘树生 | Medical centrifuge |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110102413A (en) * | 2019-05-15 | 2019-08-09 | 林伟阳 | A kind of continuous centrifugal machine for cell separation |
| CN110102413B (en) * | 2019-05-15 | 2021-11-12 | 哈尔滨工大国际干细胞工程研究院有限公司 | Continuous centrifuge for cell separation |
| CN110813557A (en) * | 2019-11-25 | 2020-02-21 | 安徽科门生物科技有限公司 | Cell centrifuge |
| CN112973975A (en) * | 2021-02-24 | 2021-06-18 | 安徽中科中佳科学仪器有限公司 | High-speed centrifuge is used in laboratory of self-cleaning filtration separation |
| CN112973975B (en) * | 2021-02-24 | 2022-08-02 | 安徽中科中佳科学仪器有限公司 | High-speed centrifuge is used in laboratory of self-cleaning filtration separation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106269302B (en) | 2018-10-12 |
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| C06 | Publication | ||
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20170104 Assignee: Anhui Xingtai Financial Leasing Co.,Ltd. Assignor: ANHUI FROM BIOLOGICAL TECHNOLOGY Co.,Ltd. Contract record no.: X2021340000019 Denomination of invention: A cell centrifugal extractor for experiment Granted publication date: 20181012 License type: Exclusive License Record date: 20210625 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A cell centrifugal extractor for experiment Effective date of registration: 20210625 Granted publication date: 20181012 Pledgee: Anhui Xingtai Financial Leasing Co.,Ltd. Pledgor: ANHUI FROM BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2021980005366 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220718 Granted publication date: 20181012 Pledgee: Anhui Xingtai Financial Leasing Co.,Ltd. Pledgor: ANHUI FROM BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2021980005366 |
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| EC01 | Cancellation of recordation of patent licensing contract | ||
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Anhui Xingtai Financial Leasing Co.,Ltd. Assignor: ANHUI FROM BIOLOGICAL TECHNOLOGY Co.,Ltd. Contract record no.: X2021340000019 Date of cancellation: 20220801 |