CN219129569U

CN219129569U - Real-time countercurrent continuous centrifuge system

Info

Publication number: CN219129569U
Application number: CN202320064857.4U
Authority: CN
Inventors: 吕新志; 刘沐芸; 杜祥熙; 郭永幸; 彭小川; 张艺; 邓云
Original assignee: Shenzhen Saidong Intelligent Manufacturing Technology Co ltd
Current assignee: Shenzhen Saidong Intelligent Manufacturing Technology Co ltd
Priority date: 2023-01-09
Filing date: 2023-01-09
Publication date: 2023-06-06
Anticipated expiration: 2033-01-09

Abstract

The embodiment of the utility model discloses a real-time countercurrent continuous centrifuge system, which comprises: the centrifugal device comprises a shell, a centrifugal structure and a liquid discharge and discharge structure, wherein the liquid discharge and discharge structure is connected with the centrifugal structure, the centrifugal structure and the liquid discharge and discharge structure are respectively connected with the shell, the centrifugal structure comprises a rotary sealing assembly and a centrifugal cone bucket, and the centrifugal cone bucket is connected with the rotary sealing assembly. The system of the embodiment of the utility model can realize the expandable centrifugal radius, acquire larger centrifugal force, reduce the manufacturing difficulty and reduce the use cost of disposable consumables.

Description

Real-time countercurrent continuous centrifuge system

Technical Field

The utility model relates to the technical field of centrifugal structures, in particular to a real-time countercurrent continuous centrifugal machine system.

Background

In biological products such as blood cells, it is often necessary to use centrifugation techniques for the separation of the different components, and in the last decades essentially conventional centrifuges have been used, i.e. several bottled containers are filled with equal amounts of the suspension to be centrifuged, and the suspension is placed in an adapter of the centrifuge for centrifugation.

The continuous flow centrifugation technology generated in recent years is designed based on the concept of a closed pipeline, can perform continuous centrifugation, and is suitable for concentrating and enriching high-capacity suspension. The typical way is two kinds, namely, a centrifugal barrel capable of rotating around the central axis of the centrifugal barrel, a piston in the centrifugal barrel is pushed by air pressure to move to suck and discharge liquid, the centrifugal barrel and other pipelines are connected through a single-channel rotary sealing structure, and the other way is counter-current centrifugation, namely, a conical centrifugal hopper is contained, the bottom and the top of the centrifugal hopper are respectively provided with two pipelines and an external pipeline which are connected through a double-channel rotary sealing structure, the liquid is pumped inwards and outwards through the bottom and the top of the peristaltic pump control cone hopper, and a camera which is arranged at the bottom and can trigger photographing is further used for acquiring the sediment aggregation condition so as to determine the time for controlling the liquid pumping. The traditional centrifuge needs to be opened, when aseptic or highly pathogenic stock solution is needed to be treated, the centrifuge needs to be strictly and normally operated by an experienced operator in a high-standard laboratory, the risk is higher, the operation is interrupted, and only a small amount of solution with fixed volume can be treated at a time, so that the efficiency is difficult to improve and the automation is difficult to realize. Axial continuous centrifugation has limited centrifuge bowl diameter due to its centrifugal force, is difficult to achieve large centrifugal forces, and is not suitable for use when the liquid volume is small. The existing centrifugal cone bucket and rotary sealing assembly integrated design of countercurrent continuous centrifugation is difficult to expand centrifugal radius, obtains larger centrifugal force, has less volume which can be accommodated by centrifugal cavity angle and shape, and has high requirements on manufacturing process and high consumable cost.

Therefore, it is necessary to design a new system to achieve an expandable centrifugal radius and obtain a larger centrifugal force, thereby reducing the manufacturing difficulty and reducing the use cost of disposable consumables.

Disclosure of Invention

The utility model aims to provide a real-time countercurrent continuous centrifugal machine system.

In order to solve the technical problems, the aim of the utility model is realized by the following technical scheme: there is provided a real-time countercurrent continuous centrifuge system comprising: the centrifugal device comprises a shell, a centrifugal structure and a liquid discharge and discharge structure, wherein the liquid discharge and discharge structure is connected with the centrifugal structure, the centrifugal structure and the liquid discharge and discharge structure are respectively connected with the shell, the centrifugal structure comprises a rotary sealing assembly and a centrifugal cone bucket, and the centrifugal cone bucket is connected with the rotary sealing assembly.

The further technical scheme is as follows: the centrifugal structure comprises a base and a driving shaft, the centrifugal cone hopper and the rotary sealing assembly are respectively connected with the driving shaft, the driving shaft is connected with the base, the liquid discharging and discharging structure is connected with the centrifugal cone hopper, and the base is connected with the shell.

The further technical scheme is as follows: the centrifugal structure further comprises a camera and a controller, wherein the camera is connected with the controller, and the camera and the controller are respectively connected to the base.

The further technical scheme is as follows: the base is arranged in the shell.

The further technical scheme is as follows: the liquid discharge structure comprises a multi-way pipeline sleeve, a pinch valve and a peristaltic pump, wherein the multi-way pipeline sleeve is connected with a liquid bag, the pinch valve is connected to the multi-way pipeline sleeve, and the peristaltic pump is connected with the multi-way pipeline sleeve.

The further technical scheme is as follows: the multi-way pipeline suite comprises an annular pipeline and a plurality of first pipelines, wherein the first pipelines are communicated with the annular pipeline, the centrifugal cone bucket is connected to the annular pipeline, the peristaltic pump is connected to the annular pipeline, and the pinch valve is connected with the first pipelines.

The further technical scheme is as follows: the liquid bag weighing device further comprises a weighing structure, wherein the weighing structure is connected with the shell, and the weighing structure is connected with the liquid bag.

The further technical scheme is as follows: the shell is provided with a through hole, and a visible protective cover is covered on the through hole.

The further technical scheme is as follows: the base is connected in the shell through a connecting rod.

The further technical scheme is as follows: the weighing structure is connected to the upper side of the shell through an upright post.

Compared with the prior art, the utility model has the beneficial effects that: according to the centrifugal cone bucket type centrifugal pump, the shell, the centrifugal structure and the liquid discharge-in and discharge structure are arranged in a separated mode, and the centrifugal cone bucket and the rotary sealing assembly are also arranged in a separated mode, so that the expandable centrifugal radius is realized, a larger centrifugal force is obtained, the manufacturing difficulty is reduced, and the use cost of disposable consumables is reduced.

The utility model is further described below with reference to the drawings and specific embodiments.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic perspective view of a real-time countercurrent continuous centrifuge system according to an embodiment of the present utility model;

fig. 2 is a schematic perspective view of a centrifugal structure according to an embodiment of the present utility model;

fig. 3 is a schematic perspective view of a multi-way pipeline set according to an embodiment of the present utility model;

the figure identifies the description:

10. a housing; 11. a visual protective cover; 20. a peristaltic pump; 30. a pinch valve; 40. a multi-channel tubing set; 50. a liquid bag; 60. a weighing structure; 61. a column; 70. a base; 80. centrifugal cone hopper; 90. a drive shaft; 91. a controller; 92. a camera; 93. a connecting rod; 41. a first pipe; 42. an annular duct.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be understood that the terms "comprises" and "comprising," when used in this specification and the appended claims, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

It is also to be understood that the terminology used in the description of the utility model herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. As used in this specification and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

It should be further understood that the term "and/or" as used in the present specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations.

Referring to fig. 1, fig. 1 is a schematic perspective view of a real-time countercurrent continuous centrifuge system according to an embodiment of the present utility model, where the system can be applied to any scene requiring centrifugation to achieve an expandable centrifugal radius, obtain a larger centrifugal force, reduce manufacturing difficulty, and reduce use cost of disposable consumables.

Referring to fig. 1, the real-time countercurrent continuous centrifuge system includes: the centrifugal cone bucket 80 is connected with the rotary seal assembly.

The centrifugal structure and the liquid discharge structure are arranged to be separated, the centrifugal radius of the centrifugal structure can be set to be any value, the centrifugal structure is not limited by the liquid discharge structure, and larger centrifugal force is obtained or the centrifugal structure is realized at a smaller rotation speed. In addition, to centrifugal structure, can design the taper angle that enrichment efficiency is higher and the bigger shape of volume, reduced the manufacturing degree of difficulty simultaneously, reduce the use cost of disposable consumptive material.

In one embodiment, referring to fig. 2, the centrifugal structure includes a base 70, a rotary seal assembly, a driving shaft 90, and a centrifugal cone 80, wherein the centrifugal cone 80 and the rotary seal assembly are respectively connected to the driving shaft 90, the driving shaft 90 is connected to the base 70, the liquid inlet and outlet structure is connected to the centrifugal cone 80, and the base 70 is connected to the housing 10.

In this embodiment, the rotary seal assembly includes a seal housing and a connection bracket, the connection bracket is connected with the centrifugal cone 80, the seal housing is connected with the connection bracket, and the connection bracket is connected with the driving shaft 90, and the cone and the rotary seal assembly which are components of the disposable consumable are designed to be separated, so that the centrifugal radius can be arbitrarily expanded, and a larger centrifugal force can be obtained or realized at a smaller rotation speed.

In an embodiment, referring to fig. 2, the centrifugal structure further includes a camera 92 and a controller 91, the camera 92 is connected to the controller 91, and the camera 92 and the controller 91 are respectively connected to the base 70.

The camera 92 is used for acquiring the aggregation condition of the sediment in the centrifugal cone 80, the sensor triggers photographing when the centrifugal cone 80 rotates to a specific angle, the controller 91 acquires an analysis picture to determine a concentration layering boundary, and the controller 91 is used for controlling the driving shaft 90 in a feedback manner according to an operation command and a sensor signal.

In one embodiment, referring to fig. 2, the base 70 is disposed in the housing 10. Specifically, the base 70 is connected within the housing 10 by a connecting rod 93.

In one embodiment, referring to fig. 1, the fluid intake and exhaust structure includes a multi-way tubing set 40, a pinch valve 30, and a peristaltic pump 20, wherein the multi-way tubing set 40 is connected with a fluid bag 50, the pinch valve 30 is connected to the multi-way tubing set 40, and the peristaltic pump 20 is connected to the multi-way tubing set 40.

In one embodiment, referring to fig. 3, the multi-way tubing set 40 includes an annular tube 42 and a plurality of first tubes 41, the first tubes 41 are in communication with the annular tube 42, the centrifugal cone 80 is connected to the annular tube 42, the peristaltic pump 20 is connected to the annular tube 42, and the pinch valve 30 is connected to the first tubes 41.

In one embodiment, referring to fig. 1, the real-time countercurrent continuous centrifuge system further includes a weighing structure 60, wherein the weighing structure 60 is connected to the housing 10, and the weighing structure 60 is connected to the fluid bag 50.

Specifically, the casing 10 is provided with a through hole, and the through hole is covered with a visible protective cover 11.

In one embodiment, referring to FIG. 1, the weighing structure 60 is attached to the top of the housing 10 by posts 61.

The liquid bags 50 such as cell suspension bags, harvesting bags, waste liquid bags and pure water bags are mounted on the weighing structure 60, the peristaltic pump 20 extrudes the multi-way pipeline suite 40 to pump the suspension into the centrifugal cone 80, the driving shaft 90 drives the centrifugal cone 80 to rotate and centrifuge, sediment is gathered at the bottom, cell suspension is pumped continuously and gathered continuously, the sediment is discharged to the product bags at one time, the rotary sealing assembly ensures that liquid can be added and discharged in the rotating process, the pinch valve 30 is used for changing a pipeline passage, different liquid bag sources are switched, the display screen is used for displaying a software interface and operating, the weighing structure 60 is used for acquiring the weight of the liquid bags and used for closed-loop feedback of pump liquid, the camera 92 is used for acquiring the gathering condition of the sediment in the centrifugal cone 80, the centrifugal cone 80 rotates to a specific angle, photographing is triggered through a sensor, the upper computer software acquires an analysis picture to determine a concentration layering dividing line, the controller 91 is used for controlling the driving shaft 90 according to an operating instruction and sensor signal feedback, the shell 10 is used for installing and supporting internal structures, and the protective cover 11 is used for protecting safety of personnel and equipment in operation.

The real-time countercurrent continuous centrifugal machine system comprises a shell 10, a centrifugal structure and a liquid discharge structure, wherein the centrifugal structure and the liquid discharge structure are arranged in a separated mode, and a centrifugal cone bucket 80 and a rotary sealing assembly are arranged in a separated mode, so that the centrifugal radius can be expanded, a larger centrifugal force is obtained, the manufacturing difficulty is reduced, and the use cost of disposable consumables is reduced.

While the utility model has been described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes and substitutions of equivalents may be made and equivalents will be apparent to those skilled in the art without departing from the scope of the utility model. Therefore, the protection scope of the utility model is subject to the protection scope of the claims.

Claims

1. A real-time countercurrent continuous centrifuge system, comprising: the centrifugal device comprises a shell, a centrifugal structure and a liquid discharge and discharge structure, wherein the liquid discharge and discharge structure is connected with the centrifugal structure, the centrifugal structure and the liquid discharge and discharge structure are respectively connected with the shell, the centrifugal structure comprises a rotary sealing assembly and a centrifugal cone bucket, and the centrifugal cone bucket is connected with the rotary sealing assembly.

2. The real-time counter-current continuous centrifuge system of claim 1, wherein said centrifuge structure comprises a base and a drive shaft, said centrifuge cone and said rotary seal assembly are respectively connected to said drive shaft, said drive shaft is connected to said base, said liquid discharge structure is connected to said centrifuge cone, and said base is connected to said housing.

3. The real-time countercurrent continuous centrifuge system of claim 2, wherein said centrifuge structure further comprises a camera and a controller, said camera being connected to said controller, said camera and said controller being connected to said base respectively.

4. The real time counter current continuous centrifuge system of claim 2, wherein said base is built into said housing.

5. The real-time counter-current continuous centrifuge system of claim 2, wherein said liquid discharge structure comprises a multi-pass tubing set, a pinch valve and a peristaltic pump, said multi-pass tubing set being connected with a liquid bag, said pinch valve being connected to said multi-pass tubing set, said peristaltic pump being connected to said multi-pass tubing set.

6. The real-time countercurrent continuous centrifuge system of claim 5, wherein said multi-pass tubing set comprises an annular tube and a plurality of first tubes, said first tubes being in communication with said annular tube, said centrifuge cone being connected to said annular tube, and said peristaltic pump being connected to said annular tube, said pinch valve being connected to said first tubes.

7. The real time counter current continuous centrifuge system of claim 5 further comprising a weighing structure, said weighing structure being coupled to said housing, said weighing structure being coupled to said fluid bag.

8. The real-time countercurrent continuous centrifuge system of claim 1, wherein said housing is provided with a through-hole covered with a visual protective cover.

9. The real-time counter-current continuous centrifugal system according to claim 2, wherein said base is connected in the housing by a connecting rod.

10. The real time counter current continuous centrifugal machine system according to claim 7, wherein said weighing structure is connected above said housing by means of a column.