CN211255955U - On-line continuous centrifugal device for cell culture - Google Patents
On-line continuous centrifugal device for cell culture Download PDFInfo
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- CN211255955U CN211255955U CN201921924883.XU CN201921924883U CN211255955U CN 211255955 U CN211255955 U CN 211255955U CN 201921924883 U CN201921924883 U CN 201921924883U CN 211255955 U CN211255955 U CN 211255955U
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Abstract
The utility model provides an online continuous centrifugal device for cell culture, it can solve current centrifugal device complex operation, the continuity is poor, with high costs, the technical problem that the contamination risk is high. The technical scheme is that the on-line continuous centrifugal device for cell culture comprises an end cover, a shell, a transmission mechanism, a motor and a centrifugal container, and is characterized in that: the end cover passes through first bearing rotation and sets up on the casing and with the coaxial setting of casing, drive mechanism's input and motor drive cooperation, output and end cover and casing transmission cooperation respectively, the direction of rotation of end cover and casing is the same and the rotational speed ratio is 2: 1, fixing a centrifugal container on an end cover; the on-line continuous centrifugal device further comprises a liquid inlet pipe and a liquid outlet pipe, one end of the liquid inlet pipe and one end of the liquid outlet pipe are respectively communicated with the centrifugal container, and the other end of the liquid inlet pipe and the other end of the liquid outlet pipe are arranged in parallel and jointly extend into the sleeve to be connected with the delivery pump.
Description
Technical Field
The utility model relates to a cell culture technical field, concretely relates to an online continuous centrifugal device for cell culture.
Background
The cellular immunity treatment method is to collect the human body's own immune cells, to make targeted modification, to make the number reach a certain scale through in vitro culture, then to be transfused back to the human body to kill the pathogens, cancer cells and mutated cells in the blood and tissues, to break the immune tolerance, to activate and enhance the body's immunity.
The genetically modified cell culture device for clinical cellular immunity can realize modification, screening and culture of cells. In the culture process, the separation and collection of cells are realized by a centrifugal device. Current centrifugal device is when using, adopt the centrifuging container of centrifuging tube as cell suspension, at first, after the centrifuging tube that will be equipped with the cell suspension is put into centrifuge, secondly, start centrifuge, under the effect of certain rotational speed and time, the layering is realized with culture medium or other materials to the cell, again, take out the centrifuging tube, artifical manual use pipettor separation required cell layer, relate to the transfer of centrifuging tube between different equipment and the relapse of centrifuging tube and open, close during the operation, the continuity is poor, experimenter operation error is great, and the great sample of unable continuity processing volume, and the cost is higher, and the contamination risk is high.
SUMMERY OF THE UTILITY MODEL
The utility model provides an online continuous centrifugal device for cell culture, it can solve current centrifugal device complex operation, the continuity is poor, with high costs, the technical problem that the contamination risk is high, applicable to T cell separation, cell elutriation, processes such as continuous centrifugation. To realize on-line continuous centrifugation, the problem of pipeline winding of a centrifugal mechanism is solved firstly, because the following mechanism is designed to solve the problem.
The technical scheme is that the on-line continuous centrifugal device for cell culture comprises an end cover, a shell, a transmission mechanism, a motor and a centrifugal container, and is characterized in that: the end cover is rotatably arranged on the shell through a first bearing and is coaxially arranged with the shell, the input end of the transmission mechanism is in transmission fit with the motor, the output end of the transmission mechanism is in transmission fit with the end cover and the shell respectively, the rotation directions of the end cover and the shell are the same, and the rotation speed ratio is 2: 1, the centrifugal container is fixed on the end cover;
the on-line continuous centrifugal device further comprises a liquid inlet pipe and a liquid outlet pipe, one end of the liquid inlet pipe and one end of the liquid outlet pipe are respectively communicated with the centrifugal container, the other end of the liquid inlet pipe and the other end of the liquid outlet pipe are arranged in parallel and jointly extend into the sleeve to be connected with the delivery pump, the sleeve comprises a first end part, a second end part and a main body, the first end part and the second end part are distributed from bottom to top and are coaxially arranged with the axis of the end cover, the first end part penetrates through the end cover and is movably supported by a first pipe fixing support, the first pipe fixing support is fixedly connected with the end cover, the lower part of the main body is connected with the first end part after extending into the side wall of the casing, the upper part of the main body bypasses the side wall and the end cover of the casing and is connected with the second end part, and the upper part of the main body and the adjacent part of the second end part are, the second pipe fixing bracket is fixedly connected with the shell;
the liquid inlet pipe, the liquid outlet pipe and the sleeve are hoses.
Further, the transmission mechanism comprises a first transmission shaft, a second transmission shaft, a third transmission shaft, a first-stage gear, a second-stage driving gear, a second-stage driven gear, a third-stage driving gear, a third-stage driven gear and a fourth-stage gear, the first transmission shaft is rotatably supported on a fixed seat through a second bearing and coaxially connected with the bottom of the shell, the second transmission shaft is rotatably supported on a gear set fixed support through a third bearing, the gear set fixed support is fixed at the bottom of the inner cavity of the shell, the third transmission shaft is rotatably supported on the upper portion of the gear set fixed support through a fourth bearing, the first-stage gear is fixed on the fixed seat and coaxially arranged with the transmission shaft, the second-stage driving gear and the second-stage driven gear are fixed on the second transmission shaft and distributed from bottom to top, the third-stage driving gear and the third-stage driven gear are fixed on, the four-stage gear is fixed on the end cover and coaxially arranged with the end cover, the first-stage gear is meshed with the second-stage driving gear, the second-stage driven gear is meshed with the third-stage driving gear, and the third-stage driven gear is meshed with the four-stage gear.
Furthermore, the first pipe fixing support and the second pipe fixing support are respectively provided with a movable sleeve matched with the sleeve.
Further, the second pipe fixed bolster includes fixed part, spacing portion and supporting part, the fixed part with casing fixed connection, spacing portion set up in the lateral wall outside of casing is equipped with the lower perforation that supplies the sleeve pipe to pass, the supporting part set up in the upside of end cover is equipped with the last perforation that supplies the sleeve pipe to pass, the movable sleeve install in go up the perforation.
Further, centrifugal container is the toper structure, centrifugal container axis is followed the radial setting of end cover and major part one end sets up inwards, the one end of feed liquor pipe with the diapire intercommunication of centrifugal container's stub end, the drain pipe includes cell collecting pipe and waste liquid collecting pipe, the one end of cell collecting pipe with centrifugal container's lateral wall intercommunication, waste liquid collecting pipe's one end with the roof intercommunication of centrifugal container's major part end, the other end of feed liquor pipe the other end of cell collecting pipe the other end parallel arrangement of waste liquid collecting pipe stretches into in the cover jointly.
Furthermore, the cell collecting pipe and the waste liquid collecting pipe are respectively connected with a conveying pump.
The utility model discloses an online continuous centrifugation device has following beneficial effect:
(1) through the arrangement of the liquid inlet pipe, the liquid outlet pipe and the delivery pump, the online input of cell suspension and the online collection of layered culture medium, other substances and cells are realized, and the transfer of a centrifugal container between different devices and the repeated opening and closing of the centrifugal container are avoided, so that on one hand, the centrifugal operation can be simplified, the continuous operation can be realized, the centrifugal efficiency is improved, further, the complicated mechanical arm device or corresponding manual operation is not needed, the cost is reduced, and on the other hand, the contamination risk can be reduced;
(2) the novel structural design can avoid the winding and screwing of the pipeline during centrifugation. When the device is used, the fixed seat is fixed on a shell of corresponding equipment, the shell rotates around the first transmission shaft under the action of the transmission mechanism to drive the liquid inlet pipe and the liquid outlet pipe to rotate, and the other ends of the liquid inlet pipe and the liquid outlet pipe are connected with the conveying pump, namely the other ends of the liquid inlet pipe and the liquid outlet pipe are fixed, and the other ends of the liquid inlet pipe and the liquid outlet pipe can be twisted automatically; the end cover is arranged on the shell in a rotating mode, and the centrifugal container fixed on the end cover drives one end of the liquid inlet pipe and one end of the liquid outlet pipe to be unscrewed under the action of the transmission mechanism; the first end part and the second end part are distributed from bottom to top and are coaxially arranged with the axis of the end cover, so that the liquid inlet pipe, the liquid outlet pipe and the sleeve pipe can be prevented from being pulled in the rotating process of the shell and the end cover; the liquid inlet pipe, the liquid outlet pipe and the sleeve are hoses, and the first pipe fixing support and the second pipe fixing support are movably matched with the sleeve, so that the untwisting can be transmitted to the other end through the corresponding pipe bodies of the liquid inlet pipe, the liquid outlet pipe and the sleeve; because the end cover is movably arranged on the shell, the rotation directions of the end cover and the shell are the same, and the rotation speed ratio is 2: 1 so that the untwisting and the self-twisting can be completely counteracted.
Drawings
Fig. 1 is a schematic sectional view of the present invention.
Fig. 2 is a schematic perspective view of a first viewing angle according to the present invention.
Fig. 3 is a schematic perspective view of a second perspective view of the present invention, in which the end cap and the first bearing are omitted.
Detailed Description
As shown in fig. 1 to 3, an on-line continuous centrifuge for cell culture comprises an end cap 2, a housing 1, a transmission mechanism, a motor and a centrifuge container 21, wherein the end cap 2 is rotatably arranged on the housing 1 through a first bearing 3 and is coaxially arranged with the housing 1, in fig. 3, 1b is a bearing assembly groove arranged at the upper end of the housing, and the centrifuge container 21 is fixed on the end cap 2 through a base 22;
the on-line continuous centrifugal device also comprises a liquid inlet pipe 23 and a liquid outlet pipe 24, one end of the liquid inlet pipe 23 and one end of the liquid outlet pipe 24 are respectively communicated with the centrifugal container 21, the other end of the liquid inlet pipe and the other end of the liquid outlet pipe 24 are arranged in parallel and jointly extend into the sleeve pipe 20 and then are connected with the delivery pump, the liquid inlet pipe 23, the liquid outlet pipe 24 and the sleeve pipe 20 are all hoses, the sleeve pipe 20 comprises a first end part 20a, a second end part 20b and a main body 20c, the first end part 20a and the second end part 20b are distributed from bottom to top and are coaxially arranged with the axis of the end cover 2, the first end part 20a penetrates through a through hole 1a in the center of the end cover and is movably supported by a first pipe fixing support 17, the first pipe fixing support 17 is fixedly connected with the end cover 2, the lower part of the main body 20c extends into the first end part 20a from the side wall of the shell 1, the, the upper part of the main body 20c and the part adjacent to the second end 20b are movably supported by a second pipe fixing bracket 19, and the second pipe fixing bracket 19 is fixedly connected with the shell 1;
the input end of the transmission mechanism is in transmission fit with the motor, the output end of the transmission mechanism is in transmission fit with the shell 1 respectively, concretely, the transmission mechanism comprises a first transmission shaft 4, a second transmission shaft 13, a third transmission shaft 14, a first-stage gear 7, a second-stage driving gear 8, a second-stage driven gear 9, a third-stage driving gear 10, a third-stage driven gear 11 and a fourth-stage gear 12, the first transmission shaft 4 is rotatably supported on a fixed seat 6 through a second bearing 5 and is coaxially connected with the bottom of the shell 1, the second transmission shaft 13 is rotatably supported on a gear set fixed support 15 through a third bearing 16, the gear set fixed support 15 is fixed at the bottom of an inner cavity of the shell 1, the third transmission shaft 14 is rotatably supported on the upper portion of the gear set fixed support 15 through a fourth bearing, the first-stage gear 7 is fixed on the fixed seat 6 and is coaxially, tertiary driving gear 10 and tertiary driven gear 11 are fixed in on third transmission shaft 14 and distribute from bottom to top, level four gear 12 is fixed in end cover 2 and with the coaxial setting of end cover 2, first transmission shaft 4 is connected with the pivot of motor, the meshing of one-level gear 7 and the meshing of second grade driving gear 8, second grade driven gear 9 and the meshing of tertiary driving gear 10, tertiary driven gear 11 and the meshing of level four gear 12, the direction of rotation of end cover 2 and casing 1 is the same and rotational speed (linear velocity) than being 2: 1.
the first pipe fixing bracket 17 and the second pipe fixing bracket 19 are respectively provided with a movable sleeve 18 which is matched with a sleeve 20 so as to fix the sleeve.
The second tube fixing support 19 comprises a fixing portion, a limiting portion 19a and a supporting portion 19b, the fixing portion is fixedly connected with the shell 1, the limiting portion 19a is arranged on the outer side of the side wall of the shell 1 and provided with a lower through hole for the sleeve 20 to pass through, the supporting portion 19b is arranged on the upper side of the end cover 2 and provided with an upper through hole for the sleeve 20 to pass through, and the movable sleeve is arranged on the upper through hole. Through the specific arrangement of the second tube fixing support 19, the sleeve 20 and the liquid inlet tube and the liquid outlet tube inside the sleeve are prevented from rubbing against the shell 1 and the end cover 2.
Claims (6)
1. The utility model provides an online continuous centrifugal device for cell culture, its includes end cover, casing, drive mechanism, motor and centrifugation container, its characterized in that: the end cover is rotatably arranged on the shell through a first bearing and is coaxially arranged with the shell, the input end of the transmission mechanism is in transmission fit with the motor, the output end of the transmission mechanism is in transmission fit with the end cover and the shell respectively, the rotation directions of the end cover and the shell are the same, and the rotation speed ratio is 2: 1, the centrifugal container is fixed on the end cover;
the on-line continuous centrifugal device further comprises a liquid inlet pipe and a liquid outlet pipe, one end of the liquid inlet pipe and one end of the liquid outlet pipe are respectively communicated with the centrifugal container, the other end of the liquid inlet pipe and the other end of the liquid outlet pipe are arranged in parallel and jointly extend into the sleeve to be connected with the delivery pump, the sleeve comprises a first end part, a second end part and a main body, the first end part and the second end part are distributed from bottom to top and are coaxially arranged with the axis of the end cover, the first end part penetrates through the end cover and is movably supported by a first pipe fixing support, the first pipe fixing support is fixedly connected with the end cover, the lower part of the main body is connected with the first end part after extending into the side wall of the casing, the upper part of the main body bypasses the side wall and the end cover of the casing and is connected with the second end part, and the upper part of the main body and the adjacent part of the second end part are, the second pipe fixing bracket is fixedly connected with the shell;
the liquid inlet pipe, the liquid outlet pipe and the sleeve are hoses.
2. An in-line continuous centrifuge apparatus for cell culture according to claim 1, wherein: the transmission mechanism comprises a first transmission shaft, a second transmission shaft, a third transmission shaft, a first-stage gear, a second-stage driving gear, a second-stage driven gear, a third-stage driving gear, a third-stage driven gear and a fourth-stage gear, the first transmission shaft is rotatably supported on a fixed seat through a second bearing and coaxially connected with the bottom of the shell, the second transmission shaft is rotatably supported on a gear set fixed support through a third bearing, the gear set fixed support is fixed at the bottom of the inner cavity of the shell, the third transmission shaft is rotatably supported on the upper part of the gear set fixed support through a fourth bearing, the first-stage gear is fixed on the fixed seat and coaxially arranged with the transmission shaft, the second-stage driving gear and the second-stage driven gear are fixed on the second transmission shaft and distributed from bottom to top, the third-stage driving gear and the third-stage driven gear are, the four-stage gear is fixed on the end cover and coaxially arranged with the end cover, the first-stage gear is meshed with the second-stage driving gear, the second-stage driven gear is meshed with the third-stage driving gear, and the third-stage driven gear is meshed with the four-stage gear.
3. An in-line continuous centrifuge apparatus for cell culture according to claim 1, wherein: the first pipe fixing support and the second pipe fixing support are respectively provided with a movable sleeve matched with the sleeve.
4. An in-line continuous centrifuge apparatus for cell culture according to claim 1, wherein: the second pipe fixed bolster includes fixed part, spacing portion and supporting part, the fixed part with casing fixed connection, spacing portion set up in the lateral wall outside of casing is equipped with the lower perforation that supplies the sleeve pipe to pass, the supporting part set up in the upside of end cover is equipped with the last perforation that supplies the sleeve pipe to pass, the movable sleeve install in go up the perforation.
5. An in-line continuous centrifuge apparatus for cell culture according to claim 1, wherein: centrifugal container is the toper structure, centrifugal container axis is followed the radial setting of end cover and major part one end sets up inwards, the one end of feed liquor pipe with the diapire intercommunication of centrifugal container's minor part end, the drain pipe includes cell collecting pipe and waste liquid collecting pipe, the one end of cell collecting pipe with centrifugal container's lateral wall intercommunication, the one end of waste liquid collecting pipe with the roof intercommunication of centrifugal container's major part end, the other end of feed liquor pipe the other end of cell collecting pipe the other end parallel arrangement of waste liquid collecting pipe stretches into the cover intraductally jointly.
6. An in-line continuous centrifuge apparatus for cell culture according to claim 5, wherein: the cell collecting pipe and the waste liquid collecting pipe are respectively connected with a delivery pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921924883.XU CN211255955U (en) | 2019-11-10 | 2019-11-10 | On-line continuous centrifugal device for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921924883.XU CN211255955U (en) | 2019-11-10 | 2019-11-10 | On-line continuous centrifugal device for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211255955U true CN211255955U (en) | 2020-08-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921924883.XU Active CN211255955U (en) | 2019-11-10 | 2019-11-10 | On-line continuous centrifugal device for cell culture |
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| Country | Link |
|---|---|
| CN (1) | CN211255955U (en) |
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- 2019-11-10 CN CN201921924883.XU patent/CN211255955U/en active Active
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