CN110665651A - Automatic draw type cell experiment centrifuge - Google Patents

Automatic draw type cell experiment centrifuge Download PDF

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Publication number
CN110665651A
CN110665651A CN201910974295.5A CN201910974295A CN110665651A CN 110665651 A CN110665651 A CN 110665651A CN 201910974295 A CN201910974295 A CN 201910974295A CN 110665651 A CN110665651 A CN 110665651A
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CN
China
Prior art keywords
test tube
centrifugal
type cell
belt wheel
rotating frame
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910974295.5A
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Chinese (zh)
Inventor
钱卫斌
蔡欣蕊
钱秋海
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Affiliated Hospital of Shandong University of Traditional Chinese Medicine
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Affiliated Hospital of Shandong University of Traditional Chinese Medicine
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Publication date
Application filed by Affiliated Hospital of Shandong University of Traditional Chinese Medicine filed Critical Affiliated Hospital of Shandong University of Traditional Chinese Medicine
Priority to CN201910974295.5A priority Critical patent/CN110665651A/en
Publication of CN110665651A publication Critical patent/CN110665651A/en
Pending legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B2005/0435Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles with adapters for centrifuge tubes or bags

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  • Centrifugal Separators (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses an automatic extraction type cell experiment centrifugal machine, and mainly relates to the technical field of biological cell experiments. Comprises a base, a bracket, a centrifugal base shell, a centrifugal rotating stand, a rotating stand driving device assembly, a test tube placing rack and a test tube to be centrifuged; the test tube automatic rotating device assembly is also included; the test tube autorotation device assembly comprises a hub motor, a driving gear, a driven gear, a driving belt wheel, a transmission belt and a driven belt wheel; driven pulley rotates to connect and sets up on test tube rack, it places the round hole to be equipped with the test tube on the driven pulley, treat that centrifugal test tube bottom is equipped with the sealing plug. The invention has the beneficial effects that: the test tube to be centrifuged can be more thoroughly mixed and stirred by optimizing the motion track of the test tube to be centrifuged; and the material of test tube bottom is effectual taken out completely behind can more thorough will centrifuging, improves centrifugal efficiency greatly.

Description

Automatic draw type cell experiment centrifuge
Technical Field
The invention relates to the technical field of biological cell experiments, in particular to an automatic extraction type cell experiment centrifugal machine.
Background
Centrifuges are the basic separation apparatus of biological laboratories and are commonly used to separate the solid and liquid phases of colloidal solutions.
The prior art is basically the technology of the invention patent with the application number of 201610643787.2, in which a centrifugal process is performed by rotating a centrifugal rotating frame to drive a test tube to be centrifuged to revolve around the center of the centrifugal rotating frame. But the effect of inertia is received, when waiting centrifugal test tube uniform velocity circular motion, the acceleration is all centripetal acceleration, does not have the acceleration in tangential direction, just can not play fine stirring mixing's effect. And the material with large specific gravity at the bottom of the test tube after centrifugation is extracted by controlling the lifting mechanism in the technology, thereby ensuring the separation effect. The lifting mechanism and the extraction port are unreasonable in arrangement, the lifting mechanism is complex in structure, the extraction effect can not be well achieved basically, the extraction port is not arranged at the bottommost part of the test tube, the substances at the bottom of the test tube can not be effectively extracted completely, and the centrifugal efficiency is greatly influenced.
Disclosure of Invention
The invention aims to provide an automatic extraction type cell experiment centrifuge which can more thoroughly mix and stir test tubes to be centrifuged by optimizing the motion track of the test tubes to be centrifuged; and the material of test tube bottom is effectual taken out completely behind can more thorough will centrifuging, improves centrifugal efficiency greatly.
In order to achieve the purpose, the invention is realized by the following technical scheme:
an automatic extraction type cell experiment centrifuge comprises a base, a bracket, a centrifugal base shell, a centrifugal rotating stand, a rotating stand driving device assembly, a test tube placing rack and a test tube to be centrifuged; the test tube rack is arranged around the centrifugal rotating stand in an annular array manner, and the test tube with the centrifugal tubes is arranged on the test tube rack;
the test tube automatic rotating device assembly is also included; the test tube autorotation device assembly comprises a hub motor, a driving gear, a driven gear, a driving belt wheel, a transmission belt and a driven belt wheel; the test tube placing rack is characterized in that the hub motor is arranged in the centrifugal rotating rack, the hub motor drives the driving gear to rotate, the driving gear drives the driven gear to rotate, the driven gear and the driving belt wheel are arranged in a coaxial and integrated mode, the driving belt wheel drives the driven belt wheel to rotate through a transmission belt, the driven belt wheel is rotatably connected and arranged on the test tube placing rack, a circular hole is formed in the test tube placing rack, a test tube placing circular hole is formed in the driven belt wheel, the circular hole and the test tube placing circular hole are arranged in a concentric mode, and the diameter of the circular hole and the diameter of the test tube placing circular hole are larger than the diameter of; the bottom of the test tube to be centrifuged is provided with a sealing rubber plug, and the centrifuge base shell is provided with an extraction guide tube corresponding to the sealing rubber plug.
The centrifugal seat is characterized in that a shaft through hole is formed in the bottom of the shell of the centrifugal seat, the rotating frame driving device assembly comprises a servo motor and a main shaft, the servo motor is arranged on the base and drives the main shaft to rotate, and the top end of the main shaft penetrates through the shaft through hole to be connected with the bottom of the centrifugal rotating frame.
The centrifugal base machine is characterized in that a bearing end cover is arranged at the bottom of the centrifugal base machine shell, a thrust bearing which is matched with the main shaft is arranged in the bearing end cover, a flange which is matched with the thrust bearing is arranged on the main shaft, and the two thrust bearings are respectively arranged on two sides of the flange.
And a pressing wheel is arranged at the top of the side wall of the centrifugal rotating frame and used for pressing the transmission belt.
The test tube placing rack is provided with a circular sliding groove, the driven belt wheel is provided with a sliding rail which is in sliding connection with the circular sliding groove, and the sliding rail moves circumferentially along the circular sliding groove.
The test tube is placed round hole internal surface and is equipped with the elastic rubber packing ring.
And a shell cover is arranged at the top of the centrifugal seat shell.
Compared with the prior art, the invention has the beneficial effects that:
the test tube motion of treating of this device includes rotation and revolution, and the rotation drives through test tube rotation device assembly, and the revolution is exactly that the rotation of centrifugation revolving rack drives, and this just makes the motion of treating the centrifugal test tube more become complicated, treats the optimization of centrifugal test tube movement track through adopting this kind of mode, and continuous through changing in-wheel motor, servo motor's rotational speed moreover, just can be more thorough treat that centrifugal test tube mixes and stir.
And through inserting the extraction needle tubing along the extraction stand pipe, the syringe needle is inserted and is worn the plug that seals of treating centrifugation test tube bottom, just can extract the material of test tube bottom, seals the plug setting at the test tube bottommost, can be more thorough with the complete effectual extraction of material of centrifugation back test tube bottom, improves centrifugal efficiency greatly.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
FIG. 2 is an enlarged view of portion A of FIG. 1 according to the present invention.
FIG. 3 is an enlarged view of portion B of FIG. 1 in accordance with the present invention.
Reference numerals shown in the drawings:
1. a base; 2. a support; 3. a centrifuge base housing; 4. centrifuging and rotating the frame; 5. placing a test tube rack; 6. centrifuging the test tube; 7. a hub motor; 8. a driving gear; 9. a driven gear; 10. a drive pulley; 11. a drive belt; 12. a driven pulley; 13. a circular hole; 14. placing the test tube in the round hole; 15. sealing the rubber plug; 16. extracting the guide pipe; 17. shaft perforation; 18. a servo motor; 19. a main shaft; 20. a bearing end cap; 21. a thrust bearing; 22. a flange; 23. a pinch roller; 24. a circular chute; 25. a slide rail; 26. an elastic rubber gasket; 27. and a shell cover.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and these equivalents also fall within the scope of the present application.
The invention relates to an automatic extraction type cell experiment centrifugal machine, which comprises a main body structure, a centrifugal base shell, a centrifugal rotating frame, a rotating frame driving device assembly, a test tube placing frame and a test tube to be centrifuged 6, wherein the main body structure comprises a base 1, a support 2, a centrifugal base shell 3, a centrifugal rotating frame 4, a rotating frame driving device assembly, a test tube placing frame and a test tube to be centrifuged; the rack 2 is arranged on the base 1, the bottom of the centrifugal seat shell 3 is supported by the rack 2, the centrifugal rotating frame 4 is arranged in the centrifugal seat shell 3, the rotating frame driving device assembly drives the centrifugal rotating frame 4 to rotate, the test tube placing frames 5 are arranged around the centrifugal rotating frame 4 in an annular array mode, and the test tube placing frames 5 with the centrifugal tubes are arranged on the test tube placing frames 5; the bottom of centrifugal seat casing 3 is equipped with axle perforation 17, revolving rack drive arrangement assembly includes servo motor 18 and main shaft 19, servo motor 18 sets up on base 1, servo motor 18 drive main shaft 19 rotates, the main shaft 19 top is passed axle perforation 17 and is connected with centrifugal revolving rack 4 bottom. The centrifugal seat is characterized in that a bearing end cover 20 is arranged at the bottom of the centrifugal seat shell 3, a thrust bearing 21 matched with the main shaft 19 is arranged in the bearing end cover 20, a flange 22 matched with the thrust bearing 21 is arranged on the main shaft 19, and the two thrust bearings 21 are respectively arranged on two sides of the flange 22.
The above is the technical principle of the revolution of the test tube 6 to be centrifuged. The device also comprises a test tube rotation device assembly.
The test tube rotation device assembly comprises a hub motor 7, a driving gear 8, a driven gear 9, a driving belt wheel 10, a transmission belt 11 and a driven belt wheel 12; the test tube placing device is characterized in that the hub motor 7 is arranged in the centrifugal rotating frame 4, the hub motor 7 drives the driving gear 8 to rotate, the driving gear 8 rotates to drive the driven gear 9 to rotate, the driven gear 9 and the driving pulley 10 are coaxially and integrally arranged, the driving pulley 10 drives the driven pulley 12 to rotate through the transmission belt 11, the driven pulley 12 is rotatably connected and arranged on the test tube placing frame 5, a circular hole 13 is formed in the test tube placing frame 5, a test tube placing circular hole 14 is formed in the driven pulley 12, the circular hole 13 and the test tube placing circular hole 14 are concentrically arranged, and the diameter of the circular hole 13 and the test tube placing circular hole 14 is larger than that of a test tube 6 to be centrifuged; the bottom of the test tube 6 to be centrifuged is provided with a sealing rubber plug 15, and the centrifugal seat shell 3 is provided with an extraction guide tube 16 corresponding to the sealing rubber plug 15.
And a pressing wheel 23 is arranged at the top of the side wall of the centrifugal rotating frame 4, and the driving belt 11 is pressed by the pressing wheel 23.
Be equipped with circular spout 24 on the test tube rack 5, be equipped with on the driven pulley 12 with 24 sliding connection's of circular spout slide rail 25, slide rail 25 is circular motion along circular spout 24. In this way, the driven pulley 12 can be placed on the test tube placing rack 5 and can concentrically rotate.
The test tube placing round hole 14 is internally provided with an elastic rubber gasket 26, and the elastic deformation capacity of the elastic rubber gasket 26 can stably fix the test tube 6 to be centrifuged.
The top of the centrifugal seat shell 3 is provided with a shell cover 27.
Methods of use and principles:
the movement of the test tube 6 to be centrifuged of the device comprises rotation and revolution. The rotation drives through test tube rotation device assembly, and in-wheel motor 7 drives driving gear 8 and rotates, and driving gear 8 drives driven gear 9 and rotates, thereby driven pulley 12 sets up with drive pulley 10 integration and drives drive pulley 10 and rotate, and drive pulley 10 drives driven pulley 12 through driving belt 11 and rotates to treat centrifugal test tube 6 rotation on the drive driven pulley 12. The revolution is driven by the rotation of the centrifugal rotating frame 4, and the servo motor 18 drives the centrifugal rotating frame 4 to rotate through the main shaft 19.
The movement of the test tube 6 to be centrifuged comprises rotation and revolution, so that the movement of the test tube 6 to be centrifuged is more complicated, the movement track of the test tube 6 to be centrifuged is optimized by adopting the mode, and the rotation speeds of the hub motor 7 and the servo motor 18 are continuously changed, so that the test tube 6 to be centrifuged can be more thoroughly mixed and stirred.
And through inserting the extraction needle tubing along extraction stand pipe 16, the syringe needle is inserted and is worn the plug 15 that seals of treating 6 bottoms of centrifugal test tube, just can extract the material of test tube bottom, seals plug 15 and sets up at the test tube bottommost, and the material that will centrifuge back test tube bottom that can be more thorough is effectual taking out completely, improves centrifugal efficiency greatly.

Claims (7)

1. An automatic extraction type cell experiment centrifuge comprises a base (1), a bracket (2), a centrifuge base shell (3), a centrifuge rotating frame (4), a rotating frame driving device assembly, a test tube placing frame (5) and a test tube (6) to be centrifuged;
the test tube rack is characterized in that the support (2) is arranged on the base (1), the bottom of the centrifugal seat shell (3) is supported by the support (2), the centrifugal rotating frame (4) is arranged in the centrifugal seat shell (3), the rotating frame driving device assembly drives the centrifugal rotating frame (4) to rotate, the test tube rack (5) is arranged around the centrifugal rotating frame (4) in an annular array mode, and the test tube rack (5) with the centrifugal tubes is arranged on the test tube rack (5);
the method is characterized in that: the test tube automatic rotating device assembly is also included; the test tube rotation device assembly comprises a hub motor (7), a driving gear (8), a driven gear (9), a driving belt wheel (10), a transmission belt (11) and a driven belt wheel (12);
the test tube placing device is characterized in that the hub motor (7) is arranged in the centrifugal rotating frame (4), the hub motor (7) drives the driving gear (8) to rotate, the driving gear (8) drives the driven gear (9) to rotate, the driven gear (9) and the driving belt wheel (10) are arranged in an integrated mode with the same axis, the driving belt wheel (10) drives the driven belt wheel (12) to rotate through a transmission belt (11), the driven belt wheel (12) is rotatably connected and arranged on the test tube placing frame (5), a circular hole (13) is formed in the test tube placing frame (5), a test tube placing circular hole (14) is formed in the driven belt wheel (12), the circular hole (13) and the test tube placing circular hole (14) are arranged in a concentric mode, and the diameter of the circular hole (13) and the test tube placing circular hole (14) is larger than the diameter of a test tube (;
treat that centrifugal test tube (6) bottom is equipped with seals plug (15), be equipped with on centrifugal seat casing (3) and seal corresponding extraction stand pipe (16) of plug (15).
2. The automatic extraction type cell experiment centrifuge as claimed in claim 1, wherein: centrifugal seat casing (3) bottom is equipped with axle perforation (17), revolving rack drive arrangement assembly includes servo motor (18) and main shaft (19), servo motor (18) set up on base (1), servo motor (18) drive main shaft (19) rotate, main shaft (19) top is passed axle perforation (17) and is connected bottom centrifugal revolving rack (4).
3. The automatic extraction type cell experiment centrifuge as claimed in claim 2, wherein: the centrifugal seat is characterized in that a bearing end cover (20) is arranged at the bottom of the centrifugal seat shell (3), a thrust bearing (21) matched with the main shaft (19) is arranged in the bearing end cover (20), a flange (22) matched with the thrust bearing (21) is arranged on the main shaft (19), and the two thrust bearings (21) are respectively arranged on two sides of the flange (22).
4. The automatic extraction type cell experiment centrifuge as claimed in claim 1, wherein: and a pressing wheel (23) is arranged at the top of the side wall of the centrifugal rotating frame (4), and the driving belt (11) is pressed by the pressing wheel (23).
5. The automatic extraction type cell experiment centrifuge as claimed in claim 1, wherein: be equipped with circular spout (24) on test tube rack (5), be equipped with on driven pulley (12) with circular spout (24) sliding connection's slide rail (25), circular motion is done along circular spout (24) in slide rail (25).
6. The automatic extraction type cell experiment centrifuge as claimed in claim 1, wherein: the inner surface of the test tube placing round hole (14) is provided with an elastic rubber gasket (26).
7. The automatic extraction type cell experiment centrifuge as claimed in claim 1, wherein: the top of the centrifugal seat shell (3) is provided with a shell cover (27).
CN201910974295.5A 2019-10-14 2019-10-14 Automatic draw type cell experiment centrifuge Pending CN110665651A (en)

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Application Number Priority Date Filing Date Title
CN201910974295.5A CN110665651A (en) 2019-10-14 2019-10-14 Automatic draw type cell experiment centrifuge

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Application Number Priority Date Filing Date Title
CN201910974295.5A CN110665651A (en) 2019-10-14 2019-10-14 Automatic draw type cell experiment centrifuge

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Publication Number Publication Date
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112210531A (en) * 2020-09-10 2021-01-12 威海见生生物技术有限公司 Stem cell induction culture solution and preparation method and application thereof
CN113617542A (en) * 2021-07-28 2021-11-09 上海乐纯生物技术有限公司 Centrifugal device for separating and preparing biological samples
CN113731650A (en) * 2021-09-08 2021-12-03 深圳市森盈生物科技有限公司 Integrated centrifugal system and method for full-automatic sheet making machine
CN115508295A (en) * 2022-10-28 2022-12-23 武汉市农业科学院 Rapid detection instrument for activity of feed enzyme preparation
CN117339772A (en) * 2023-12-06 2024-01-05 江苏康为世纪生物科技股份有限公司 Test tube centrifuge for reagent preparation

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4449964A (en) * 1983-02-17 1984-05-22 Separex Teknik Ab Decanting centrifuge
CN2920518Y (en) * 2006-06-06 2007-07-11 东莞丰裕电机有限公司 Automatic rotating table
JP4520004B2 (en) * 1999-09-17 2010-08-04 フレセニウス・ヘモケア・ベタイリグンクス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Magnetic drive device and centrifuge with magnetic drive device
CN102481493A (en) * 2009-08-11 2012-05-30 株式会社写真化学 Agitation/defoaming Device
CN204234220U (en) * 2014-10-08 2015-04-01 株式会社新基 Centrifuge and gear unit
CN106824560A (en) * 2017-03-14 2017-06-13 骏实生物科技(上海)有限公司 A kind of automatic mixing device for centrifugal platform
CN207703590U (en) * 2017-12-02 2018-08-07 深圳光彩生命工程技术有限公司 A kind of disposable centrifuge tube conveniently taking bottom liquid

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4449964A (en) * 1983-02-17 1984-05-22 Separex Teknik Ab Decanting centrifuge
JP4520004B2 (en) * 1999-09-17 2010-08-04 フレセニウス・ヘモケア・ベタイリグンクス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Magnetic drive device and centrifuge with magnetic drive device
CN2920518Y (en) * 2006-06-06 2007-07-11 东莞丰裕电机有限公司 Automatic rotating table
CN102481493A (en) * 2009-08-11 2012-05-30 株式会社写真化学 Agitation/defoaming Device
CN204234220U (en) * 2014-10-08 2015-04-01 株式会社新基 Centrifuge and gear unit
CN106824560A (en) * 2017-03-14 2017-06-13 骏实生物科技(上海)有限公司 A kind of automatic mixing device for centrifugal platform
CN207703590U (en) * 2017-12-02 2018-08-07 深圳光彩生命工程技术有限公司 A kind of disposable centrifuge tube conveniently taking bottom liquid

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112210531A (en) * 2020-09-10 2021-01-12 威海见生生物技术有限公司 Stem cell induction culture solution and preparation method and application thereof
CN112210531B (en) * 2020-09-10 2022-11-18 威海见生生物技术有限公司 Stem cell induction culture solution and preparation method and application thereof
CN113617542A (en) * 2021-07-28 2021-11-09 上海乐纯生物技术有限公司 Centrifugal device for separating and preparing biological samples
CN113731650A (en) * 2021-09-08 2021-12-03 深圳市森盈生物科技有限公司 Integrated centrifugal system and method for full-automatic sheet making machine
CN115508295A (en) * 2022-10-28 2022-12-23 武汉市农业科学院 Rapid detection instrument for activity of feed enzyme preparation
CN117339772A (en) * 2023-12-06 2024-01-05 江苏康为世纪生物科技股份有限公司 Test tube centrifuge for reagent preparation
CN117339772B (en) * 2023-12-06 2024-03-05 江苏康为世纪生物科技股份有限公司 Test tube centrifuge for reagent preparation

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Application publication date: 20200110