CN211111991U - Tissue source cell filter equipment - Google Patents
Tissue source cell filter equipment Download PDFInfo
- Publication number
- CN211111991U CN211111991U CN201921998157.2U CN201921998157U CN211111991U CN 211111991 U CN211111991 U CN 211111991U CN 201921998157 U CN201921998157 U CN 201921998157U CN 211111991 U CN211111991 U CN 211111991U
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- annular
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- vertical rod
- box body
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- 238000001914 filtration Methods 0.000 claims abstract description 31
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 210000001519 tissue Anatomy 0.000 abstract description 29
- 238000002360 preparation method Methods 0.000 abstract description 10
- 238000000926 separation method Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 7
- 210000004877 mucosa Anatomy 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 23
- 238000005070 sampling Methods 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 3
- 210000003701 histiocyte Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000001085 differential centrifugation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000009461 vacuum packaging Methods 0.000 description 1
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Abstract
The utility model discloses a tissue source cell filter equipment, relate to histocyte and draw separation technical field, the on-line screen storage device comprises a base, the montant, the horizontal pole, the mounting panel, the box body, miniature vibrating motor, the receiver, a plurality of filter screens and at least three annular filter tube, a plurality of annular filter tubes from top to bottom joint in proper order, be provided with a filter screen between two adjacent annular filter tubes, the mesh number of a plurality of filter screens increases from top to bottom in proper order, the box body is fixed in on the bottom surface of one side that the mounting panel is close to the horizontal pole, the box body side is provided with the opening, miniature vibrating motor installs in the box body, the one end that the horizontal pole was kept away from to the mounting panel is provided with the through-hole, the annular filter tube of lower extreme. The device easy operation, effective filtration area is big, improves preparation efficiency, has effectively avoided tissue piece or mucosa etc. to pile up and blocks up the filter screen mouth and lead to influencing the filter effect.
Description
Technical Field
The utility model relates to a histocyte draws separation technical field, especially relates to a tissue source cell filter equipment.
Background
When the cell separation and extraction is performed from a tissue, the obtained cell product generally has original tissues, cell masses, connective tissues, mucous membranes, and the like which are not completely dispersed, in addition to the cells obtained. At present, most of separation methods commonly used in laboratories filter a histiocyte solution to be separated into a centrifugal tube for multiple times or naturally settle and perform differential centrifugation, but when tissue blocks are more and the volume is larger, the filtration times are more and the time consumption is long, longer time is needed for completely separating and obtaining a cell suspension, the operation steps of separation and filtration are complicated, microbial pollution is easily caused, and the separation and the culture of the primary cells of the subsequent tissue source are not facilitated.
Therefore, it is an urgent technical problem to provide a cell filtration device, which can filter and screen out tissue blocks and other large particulate matters, not only can reduce complexity in the operation process, but also can greatly reduce microbial contamination caused by complicated operation and long operation time in the cell preparation process, and improve preparation efficiency.
SUMMERY OF THE UTILITY MODEL
For solving above technical problem, the utility model provides a tissue source cell filter equipment, easy operation, effective filtration area is big, improves preparation efficiency, has effectively avoided tissue piece or mucosa etc. to pile up and blocks up the filter screen mouth and lead to influencing the filter effect.
In order to achieve the above object, the utility model provides a following scheme:
the utility model provides a tissue source cell filtering device, which comprises a base, a vertical rod, a cross rod, a mounting plate, a box body, a micro vibration motor, a receiver, a plurality of filter screens and at least three annular filter tubes, wherein the annular filter tubes are sequentially clamped from top to bottom, one filter screen is arranged between two adjacent annular filter tubes, the mesh number of the filter screens is sequentially increased from top to bottom, the vertical rod is fixed on the base, the cross rod is arranged on the upper part of the vertical rod, the mounting plate is fixed at one end of the cross rod, the mounting plate is positioned above the base, the box body is fixed on the bottom surface of one side of the mounting plate close to the cross rod, the side surface of the box body is provided with an opening, the micro vibration motor is arranged in the box body, one end of the mounting plate, which is far, the annular filter pipe at the lowermost end penetrates through the through hole and extends to the lower part of the mounting plate, the receiver is arranged at the bottom end of the annular filter pipe at the lowermost end, and the receiver is placed on the base.
Preferably, the annular filter pipe includes first ring pipe and second ring pipe, first ring pipe is fixed in second ring pipe upper portion, the external diameter of first ring pipe is greater than the external diameter of second ring pipe, the upper portion of the inside wall of first ring pipe is provided with annular groove, the second ring pipe with annular groove structure phase-match, the filter screen joint in upper portion annular filter pipe the second ring pipe and lower part annular filter pipe between the annular groove.
Preferably, the second circular ring tube of the lowermost annular filter tube is capable of extending into the receptacle.
Preferably, a handle is fixed on the outer wall of the first circular ring pipe.
Preferably, the mounting panel includes connecting plate and support ring, the connecting plate is fixed in horizontal pole one end, support ring is fixed in the connecting plate is kept away from the one end of horizontal pole, support ring with second ring tubular construction phase-match, the box body is fixed in the connecting plate bottom surface.
Preferably, the fixing device further comprises a bolt, the vertical rod is of a hollow structure, a mounting hole penetrating through the side face of the vertical rod is formed in the upper portion of the vertical rod, the cross rod penetrates through one side of the vertical rod and extends to the other side of the vertical rod, and the bolt penetrates through the top face of the vertical rod and extends to the inside of the vertical rod and is in contact with the cross rod.
Preferably, the miniature vibration motor further comprises a button switch, a rechargeable battery and a charging port, the miniature vibration motor is connected with the rechargeable battery through a wire, the button switch is arranged on the wire, the charging port is connected with the rechargeable battery, the base is of a hollow structure, the rechargeable battery is located inside the base, the charging port is arranged on the side face of the base, and the button switch is arranged on the upper surface of the base.
Preferably, the receptacle is a disposable sterile sampling bottle.
The utility model discloses for prior art gain following technological effect:
the utility model provides a tissue source cell filter equipment, a plurality of annular filter pipes from top to bottom joint in proper order is provided with a filter screen between two adjacent annular filter pipes, and the mesh number of a plurality of filter screens increases from top to bottom in proper order, and on the box body was fixed in the bottom surface of one side that the mounting panel was close to the horizontal pole, the box body side was provided with the opening, and miniature vibrating motor installed in the box body. The device is simple to operate, and effective filtration area is big, carries out multiple filtration simultaneously, and has connected vibrating device, has effectively avoided tissue piece or mucosa etc. to pile up and blocks up the filter screen mouth and lead to influencing the filter effect, under relative sealed condition, accomplishes multistep filtration in the short time, improves preparation efficiency, is applicable to the preparation of tissue source primary cell and the separation of tissue source cell.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a front view of a tissue-derived cell filtration device provided by the present invention;
FIG. 2 is a top view of the tissue-derived cell filtration device provided by the present invention without the annular filter tube and the receiver;
FIG. 3 is a front view of the ring filter tube and the handle of the present invention;
FIG. 4 is a cross-sectional view of the middle ring filter tube of the present invention;
FIG. 5 is a top view of the middle ring filter tube and the handle of the present invention;
fig. 6 is a schematic view of the middle ring-shaped filtering pipe of the present invention when a filtering net is installed therein.
Description of reference numerals: 1. a base; 2. a vertical rod; 3. a cross bar; 4. a connecting plate; 5. a support ring; 6. an annular filter tube; 61. a first annular tube; 62. a second circular tube; 63. a circular groove; 7. a handle; 8. a receiver; 9. a box body; 10. a micro vibration motor; 11. an electric wire; 12. a bolt; 13. a charging port; 14. a push button switch; 15. and (4) a filter screen.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model aims at providing a tissue source cell filter equipment, easy operation, effective filtration area is big, improves preparation efficiency, has effectively avoided tissue piece or mucosa etc. to pile up and blocks up the filter screen mouth and lead to influencing the filter effect.
In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.
As shown in fig. 1-2, this embodiment provides a tissue-derived cell filtering apparatus, which includes a base 1, a vertical rod 2, a horizontal rod 3, a mounting plate, a box 9, a micro-vibration motor 10, a receiver 8, a plurality of filter screens 15, and at least three annular filter pipes 6, wherein the plurality of annular filter pipes 6 are sequentially clamped from top to bottom, one filter screen 15 is disposed between two adjacent annular filter pipes 6, the mesh number of the plurality of filter screens 15 increases from top to bottom, the vertical rod 2 is fixed on the base 1, the horizontal rod 3 is mounted on the upper portion of the vertical rod 2, the mounting plate is fixed at one end of the horizontal rod 3 and is located above the base 1, the box 9 is fixed on the bottom surface of the mounting plate near one side of the horizontal rod 3, an opening is disposed on the side surface of the box 9, the micro-vibration motor 10 is mounted in the box 9, specifically, the annular filter pipe 6 at the lowest end passes through the through hole and extends to the lower part of the mounting plate, a receiver 8 is arranged at the bottom end of the annular filter pipe 6 at the lowest end, and the receiver 8 is placed on the base 1. The device is simple to operate, and effective filtration area is big, carries out multiple filtration simultaneously, and has connected vibrating device, has effectively avoided tissue piece or mucosa etc. to pile up and blocks up the filter screen mouth and lead to influencing the filter effect, under relative sealed condition, accomplishes multistep filtration in the short time, improves preparation efficiency, is applicable to the preparation of tissue source primary cell and the separation of tissue source cell.
As shown in fig. 3-6, annular filter pipe 6 includes first ring pipe 61 and second ring pipe 62, first ring pipe 61 is fixed in second ring pipe 62 upper portion, the external diameter of first ring pipe 61 is greater than the external diameter of second ring pipe 62, the internal diameter of first ring pipe 61 is the same with the internal diameter of second ring pipe 62, the upper portion of the inside wall of first ring pipe 61 is provided with annular groove 63, second ring pipe 62 and annular groove 63 structural phase-match, specifically, filter screen 15 in this embodiment is circular filter screen, filter screen 15 joint is between annular filter pipe 6's second ring pipe 62 on upper portion and annular filter pipe 6's of lower part annular groove 63, play firm support, press from both sides tight firm effect to filter screen 15. When the filter screen 15 needs to be taken down, the first circular ring pipe 61 is only required to be rotated to separate the adjacent circular filter pipes 6.
In the present embodiment, the outer diameter of the first circular tube 61 is 56.15mm, the inner diameter is 54.36mm, and the height is 20 mm; the outer diameter of the second circular tube 62 is 55mm, and the diameter of the circular groove 63 is 55 mm.
In particular, the second circular ring tube 62 of the lowermost annular filter tube 6 can extend into the receptacle 8. The mouth of the receptacle 8 for receiving the filtrate has an inner diameter slightly larger than the outer diameter of the second circular tube 62 connected to the receptacle 8 so that the lowermost circular filter tube 6 can be inserted and stably seated on the receptacle 8.
In this embodiment, the receiver 8 is a disposable sterile sampling bottle. The disposable sterile sampling bottle is a 500ml sterile single vacuum packaging disposable sampling bottle with the inner diameter of 56mm and the outer diameter of 60mm, and can be sterilized under high pressure and resist high temperature. The receiver 8 can be other receiving bottles with the inner diameter of the bottle mouth of 56mm to 59mm, and can move horizontally in any direction.
In order to facilitate the handling of the annular filter tube 6 and to ensure aseptic handling, a handle 7 is fixed to the outer wall of the first annular tube 61. In this embodiment, the length of the handle 7 is 60 mm.
The mounting panel includes connecting plate 4 and support ring 5, and connecting plate 4 is fixed in 3 one ends of horizontal pole, and support ring 5 is fixed in the one end that horizontal pole 3 was kept away from to connecting plate 4, supports ring 5 and second ring pipe 62 structure phase-matches, and box body 9 is fixed in the bottom surface of connecting plate 4. In this embodiment, the inner diameter of the support ring 5 is 55mm, and the outer diameter is 59 mm; the connecting plate 4 has a length of 50mm and a width of 34 mm.
Still include bolt 12 in this embodiment, montant 2 is hollow structure, and 2 upper portions of montant are provided with the mounting hole that runs through 2 sides of montant, and horizontal pole 3 passes the mounting hole by one side of montant 2 and extends the opposite side of montant 2, and bolt 12 passes the top surface of montant 2 and extends montant 2 inside and contact with horizontal pole 3. When the cross rod 3 needs to be moved, the bolt 12 is screwed upwards to be separated from the cross rod 3, the cross rod 3 can slide in the mounting hole according to requirements, and after the position is adjusted, the bolt 12 is screwed downwards to be pressed against the cross rod 3.
The micro vibration motor comprises a button switch 14, a rechargeable battery and a charging port 13, wherein the micro vibration motor 10 is connected with the rechargeable battery through a wire 11, the button switch 14 is arranged on the wire 11, the charging port 13 is connected with the rechargeable battery, the base 1 is of a hollow structure, the rechargeable battery is located inside the base 1, the charging port 13 is arranged on the side surface of the base 1, the button switch 14 is arranged on the upper surface of the base 1, and the button switch 14 is used for controlling the micro vibration motor 10 to be opened and closed. Specifically, the electric wire 11 passes through the stem 2 and extends through the inside of the stem 2 into the base 1.
In this embodiment, the base 1 has a length of 160mm, a width of 135mm and a thickness of 12 mm; the height of the vertical rod 2 is 160mm, and the thickness is 12 mm; the cross bar 3 is 80mm in length. Base 1, montant 2, horizontal pole 3, mounting panel, box body 9, annular filter tube 6 and handle 7 all adopt stainless steel. It should be noted that the enlargement or reduction of the size of the device is also within the scope of the present invention.
In this embodiment, the number of the annular filter pipes 6 is three, the number of the filter screens 15 is two, the number of the filter screens 15 at the upper part is 30, and the number of the filter screens 15 at the lower part is 80, so that the number of the filter screens 15 can be changed according to the use requirement.
During the use, at first, install annular filter tube 6, filter screen 15 and receiver 8, then slowly pour into the histiocyte suspension that treats the separation into the lumen of annular filter tube 6, press button switch 14, start miniature vibrating motor 10, double-deck filter screen 15 filters the tissue liquid, thereby realize that great tissue piece and the great material of volume are held back on the filter screen 15 of upper strata, and less tissue piece and cell mass are held back on the filter screen 15 of lower floor, the cell suspension flows to supporting receiver 8, when receiving certain volume, take off receiver 8, change new receiver 8 and continue to collect the purpose cell suspension, miniature vibrating motor 10 makes this filter equipment vibrate in the horizontal direction, shake annular filter tube 6, accelerate the filter speed.
To sum up, the tissue source cell filter equipment in this embodiment simple structure, convenient operation, the commonality is strong, and the replacement simple installation, convenient equipment, convenient to detach washs, and the antioxidant capacity is strong, is difficult for getting rusty, and is corrosion-resistant, high temperature resistant autoclaving, can effectively solve many times and filter the problem that brings the operating procedure complicacy, can effectively avoid the cell pollution problem, is one kind and is applicable to multiple tissue source unicellular preparation and filters, and solid particle in blood or other specimens realizes solid-liquid separation's filter equipment.
The principle and the implementation mode of the present invention are explained by applying specific examples in the present specification, and the above descriptions of the examples are only used to help understanding the method and the core idea of the present invention; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the concrete implementation and the application scope. In summary, the content of the present specification should not be construed as a limitation of the present invention.
Claims (8)
1. A tissue source cell filtering device is characterized by comprising a base, a vertical rod, a transverse rod, a mounting plate, a box body, a micro vibration motor, a receiver, a plurality of filter screens and at least three annular filter tubes, wherein the annular filter tubes are sequentially clamped from top to bottom, one filter screen is arranged between every two adjacent annular filter tubes, the mesh number of the filter screens is sequentially increased from top to bottom, the vertical rod is fixed on the base, the transverse rod is mounted on the upper portion of the vertical rod, the mounting plate is fixed at one end of the transverse rod and is positioned above the base, the box body is fixed on the bottom surface of one side, close to the transverse rod, of the mounting plate, an opening is formed in the side surface of the box body, the micro vibration motor is mounted in the box body, a through hole is formed in one end, far away from the, the annular filter pipe at the lowermost end penetrates through the through hole and extends to the lower part of the mounting plate, the receiver is arranged at the bottom end of the annular filter pipe at the lowermost end, and the receiver is placed on the base.
2. The tissue source cell filtering device according to claim 1, wherein the annular filtering tube comprises a first annular tube and a second annular tube, the first annular tube is fixed on the upper portion of the second annular tube, the outer diameter of the first annular tube is larger than that of the second annular tube, an annular groove is formed in the upper portion of the inner side wall of the first annular tube, the second annular tube is matched with the annular groove in structure, and the filtering net is clamped between the second annular tube of the annular filtering tube on the upper portion and the annular groove of the annular filtering tube on the lower portion.
3. The tissue-derived cell filtration device of claim 2, wherein the second circular ring of the lowermost annular filtration tube is capable of extending into the receptacle.
4. The tissue-derived cell filtration device of claim 2, wherein a handle is fixed to the outer wall of the first circular tube.
5. The tissue-derived cell filtration device of claim 2, wherein the mounting plate comprises a connecting plate and a support ring, the connecting plate is fixed to one end of the cross bar, the support ring is fixed to the end of the connecting plate away from the cross bar, the support ring is structurally matched with the second ring, and the box body is fixed to the bottom surface of the connecting plate.
6. The tissue-derived cell filtration device of claim 1, further comprising a bolt, wherein the vertical rod is a hollow structure, the upper portion of the vertical rod is provided with a mounting hole penetrating through the side surface of the vertical rod, the cross rod penetrates through the mounting hole from one side of the vertical rod to the other side of the vertical rod, and the bolt penetrates through the top surface of the vertical rod to extend into the vertical rod and contact with the cross rod.
7. The tissue-derived cell filtration device according to claim 6, further comprising a button switch, a rechargeable battery and a charging port, wherein the micro-vibration motor is connected to the rechargeable battery through a wire, the button switch is disposed on the wire, the charging port is connected to the rechargeable battery, the base is hollow, the rechargeable battery is located inside the base, the charging port is disposed on a side surface of the base, and the button switch is disposed on an upper surface of the base.
8. The tissue-derived cell filtration device of claim 1, wherein the receptacle is a disposable sterile sample bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921998157.2U CN211111991U (en) | 2019-11-19 | 2019-11-19 | Tissue source cell filter equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921998157.2U CN211111991U (en) | 2019-11-19 | 2019-11-19 | Tissue source cell filter equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211111991U true CN211111991U (en) | 2020-07-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921998157.2U Active CN211111991U (en) | 2019-11-19 | 2019-11-19 | Tissue source cell filter equipment |
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| Country | Link |
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| CN (1) | CN211111991U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112358958A (en) * | 2020-11-23 | 2021-02-12 | 浙江省人民医院 | Sorting and filtering device for tumor cell mass for oncology department |
| CN113834799A (en) * | 2021-07-29 | 2021-12-24 | 杭州佰迈医疗科技有限公司 | Separation system for target substance in biological liquid sample |
-
2019
- 2019-11-19 CN CN201921998157.2U patent/CN211111991U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112358958A (en) * | 2020-11-23 | 2021-02-12 | 浙江省人民医院 | Sorting and filtering device for tumor cell mass for oncology department |
| CN112358958B (en) * | 2020-11-23 | 2023-10-27 | 浙江省人民医院 | Separation and filtration device for tumor cell mass for oncology department |
| CN113834799A (en) * | 2021-07-29 | 2021-12-24 | 杭州佰迈医疗科技有限公司 | Separation system for target substance in biological liquid sample |
| CN113834799B (en) * | 2021-07-29 | 2024-12-13 | 杭州佰迈医疗科技有限公司 | Separation system of target substances in biological fluid samples |
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