CN204219887U - A kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation - Google Patents
A kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation Download PDFInfo
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- CN204219887U CN204219887U CN201420591885.2U CN201420591885U CN204219887U CN 204219887 U CN204219887 U CN 204219887U CN 201420591885 U CN201420591885 U CN 201420591885U CN 204219887 U CN204219887 U CN 204219887U
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- filter
- mesh screen
- gradient centrifugation
- density gradient
- seminal fluid
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Abstract
The utility model discloses a kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation, the top of described mesh screen is filter house, described filter house cylindrically structure, the inside of described filter house is provided with screen pack, described screen pack is mutually superposed by the identical filter of two panels and forms, and two panels filter adjusts the change of relative position between the two by the aperture adjustment knob be arranged on outside filter house; The bottom of described mesh screen is conduction part, and the upper end of described conduction part is connected with the bottom of described filter house, and the opening of the lower end of described conduction part reduces to side gradually and forms a draining hole in bottom, and the below of described draining hole is connected with drainage tube.The utility model is practical, and structure is simple, and cost is low, is easy to apply.
Description
Technical field
The utility model belongs to biochemical instrument equipment, is specifically related to a kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation.
Background technology
Be used for the conventional steps in Assisted Reproductive Techniques Treatment during sperm optimization process, object removes refining and impurity, reduces the impact of the material that impact fertilization implantation is grown, be separated the normal activity sperm with plentiful sufficient amount, improve the mobility of sperm.At present, the method that supplementary reproduction laboratory is usually used in sperm optimization process has upper reaches method and density-gradient centrifugation method, and often kind of a method has quality, also has to use in conjunction with two kinds of methods.Upper reaches method is the travelling ability utilizing sperm, enters different nutrient solutions reach sperm optimization separation volume object by liquid surface.The method have simple to operate, save the low and feature such as to have no side effect of nutrient solution, cost, but sperm motility increases not obvious, reclaims sperm concentration low, and defective sperm number is higher.This method can wash away in refining the volume material affecting sperm motility, as leucocyte, bacterium, AsAb and prostaglandin etc., is relatively applicable to the separation of eupyrene sperm.Density-gradient centrifugation method utilizes the density gradient centrifugation liquid with separate activities sperm to optimize sperm, the rate of recovery is higher, and outside process normal semen, to weak sperm less, defective sperm can obtain the higher sperm of vigor to greatest extent, be convenient to the Clinical Pregnancy Rate in improving IUI, therefore, in the few weak essence of process or defective sperm, density-gradient centrifugation method is optimized sperm and is more and more applied, density-gradient centrifugation method optimizes the general step of sperm for high density centrifugate and low-density centrifugate to be joined respectively in separator tube first respectively, then the seminal fluid to be optimized of liquefaction is added, carry out centrifugal treating, then the sperm of bottom is obtained.
But, find in the clinical testing of reality, the seminal fluid of some patients contains crystallization, containing the seminal fluid do not liquefied in the seminal fluid of some patients, this just causes these effects containing crystallization or affect density gradient centrifugation containing the seminal fluid do not liquefied, even if make, abundant high-quality sperm is also difficult to be effectively separated high-quality sperm.If the crystallization in seminal fluid or the seminal fluid that do not liquefy can be screened out before gradient density centrifugal, will greatly improve the effect of optimization of seminal fluid.
Patent CN102120111B discloses a kind of disposable filtering formula seminal fluid and is separated centrifuge tube, this utility model utilizes the semen sample of pure physical method to abnormal liquefaction to filter by arranging filter membrane in sleeve pipe, this centrifuge tube can remove fiber protein yarn, gel particle, cell component in seminal fluid, seminal fluid is liquefied completely, thus make sperm freely movable, also can remove part Xingtai abnormal spermium and part Necrospermia simultaneously.But the Main Function of this centrifuge tube only rests in the pretreatment to seminal fluid.Want it to carry out density gradient centrifugation further, need the seminal fluid after by process to transfer to further in density gradient centrifugation pipe and operate, one adds operating procedure, also easily causes damage or pollutes on the other hand in transfer process.
Utility model content
Utility model object: for solving problems of the prior art, the utility model provides a kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation, is particularly useful for the process of the seminal fluid of abnormal liquefaction.
Technical scheme: for realizing above-mentioned technical purpose, the utility model proposes a kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation, the top of described mesh screen is filter house, described filter house cylindrically structure, the inside of described filter house is provided with screen pack, described screen pack is mutually superposed by the identical filter of two panels and forms, and two panels filter adjusts the change of relative position between the two by the aperture adjustment knob be arranged on outside filter house; The bottom of described mesh screen is conduction part, and the upper end of described conduction part is connected with the bottom of described filter house, and the opening of the lower end of described conduction part reduces to side gradually and forms a draining hole in bottom, and the below of described draining hole is connected with drainage tube.
Preferably, the inside of described filter house is provided with three layer filtration net from top to bottom, and be respectively the first screen pack, the second screen pack and the 3rd screen pack, the distance between every layer of screen pack is 1 ~ 5mm.
Particularly, the filter opening aperture of each filter of described first screen pack is 30 ~ 35 μm, and the filter opening aperture of each filter of the second screen pack is 10 ~ 13 μm, and the filter opening aperture of each filter of the 3rd screen pack is 6 ~ 9 μm.
Further preferably, in order to the density gradient centrifugation pipe making described mesh screen be applicable to different bore, described mesh screen has bracing frame in the arranged outside of the junction of filter house and conduction part.
Preferably, described bracing frame protrudes from the outside 1 ~ 2cm of described filter house.
Preferably, the diameter of described drainage tube is 2 ~ 5mm.
The utility model further provides a kind of density gradient centrifugation pipe for abnormal liquefaction seminal fluid density gradient centrifugation, described density gradient centrifugation pipe comprises above-mentioned special mesh screen and density gradient centrifugation tube body, wherein, described special mesh screen is removably erected on the upper end of density gradient centrifugation tube body.
Beneficial effect: compared with prior art, private network sifter device of the present utility model has the following advantages:
(1) by arranging the adjustable screen pack in aperture, different pore sizes can be selected according to actual needs on the one hand to different semen sample, on the other hand, also can control by the size of adjustment aperture the speed that seminal fluid adds density gradient centrifugation pipe, thus make seminal fluid join the top of density gradient centrifugation liquid more slowly.
(2) pretreatment of seminal fluid and density gradient centrifugation can be integrated together by the utility model easily, directly carry out density gradient centrifugation, save the step of transfer, avoid the waste of seminal fluid after to liquefacient duration.
(3) design of mesh screen gripper shoe makes mesh screen can be applied to the test tube of various caliber size, applied range.
(4) design of drainage tube avoids seminal fluid waste residual on tube wall on the one hand, and the seminal fluid after can making filtration on the other hand joins the top of density gradient centrifugation liquid slowly, is convenient to the operation of testing.
(5) the utility model is practical, and structure is simple, and cost is low, is easy to utilization and extention.
Accompanying drawing explanation
Fig. 1 is the structural representation of the special mesh screen of the utility model;
Fig. 2 is the using state figure of special mesh screen of the present utility model;
Fig. 3 is the schematic diagrames of two filters of screen pack of the present utility model when overlapping completely;
Fig. 4 is the schematic diagram after the relative position change of two filters of screen pack of the present utility model.
Detailed description of the invention
The utility model proposes a kind of density gradient centrifugation pipe for abnormal liquefaction seminal fluid density gradient centrifugation, comprise special mesh screen and common density gradient centrifugation tube body 9, this private network screen frame (unit) is set to the upper end of density gradient centrifugation tube body 9.
As shown in Figure 1, the top of special mesh screen is filter house 1, filter house 1 cylindrically structure, the inside of filter house is provided with three layer filtration net, be respectively the first screen pack 2, second screen pack 3 and the 3rd screen pack 4, wherein, vertical range between first screen pack and the second screen pack is 5mm, distance between second screen pack and the 3rd screen pack is 3mm, every layer of screen pack is mutually superposed by the identical filter 10 of two panels and forms, two panels filter 10 adjusts the change of relative position between the two by the aperture adjustment knob 11 be arranged on outside filter house, as shown in Figure 3 and Figure 4, by changing the relative position of two filters 10, reach the object in the aperture of modified filter screen.The filter opening aperture of each filter of the first screen pack is 35 μm, and the filter opening aperture of each filter of the second screen pack is 12 μm, and the filter opening aperture of each filter of the 3rd screen pack is 8 μm
The bottom of mesh screen is conduction part 6, and the upper end of conduction part 6 is connected with the bottom of filter house 1, and the opening of the lower end of conduction part 6 reduces to side gradually and forms a draining hole 7 in bottom, and the below of draining hole 7 is connected with drainage tube 8.The diameter of drainage tube is 3mm.The shown side of drainage tube 8 and the side of filter house are on same vertical curve, and namely when being positioned on density gradient centrifugation tube body 9 by mesh screen, this drainage tube 8 is inscribe state with the inner side of density gradient centrifugation tube body 9.Arranging of drainage tube 8 avoids seminal fluid waste residual on tube wall on the one hand, and the seminal fluid after can making filtration on the other hand joins the top of density gradient centrifugation liquid slowly, is convenient to the operation of testing.
Mesh screen has bracing frame in the arranged outside of the junction of filter house and conduction part, and bracing frame protrudes about 2cm outside filter house.For easily mesh screen rack being located at the top of density gradient centrifugation tube body 9, meanwhile, by arranging bracing frame, this special mesh screen can be used for the top of the density gradient centrifugation tube body of different bore.
In use, as shown in Figure 2, first as requested density gradient centrifugation liquid is joined in density gradient centrifugation tube body respectively, observe semen sample, judge that this seminal fluid belongs to containing the seminal fluid of crystallization or containing the seminal fluid as liquefaction, then the aperture of each layer filter of mesh screen is regulated, such as, if contain the seminal fluid of crystallization, then the position of the two-layer filter of the first screen pack can regulate, namely both overlap completely, shown in Fig. 3, slightly regulate the position (as shown in Figure 4) of the two-layer filter of the second screen pack, to slow down the falling speed of seminal fluid, then as the case may be, the relative position of two filters of the 3rd screen pack can be selected further, then mesh screen rack is set to the upper end of density gradient centrifugation tube body, movable support plate, make the madial wall of drainage tube and density gradient centrifugation pipe tangent, thus the seminal fluid of process can be added drop-wise in density gradient centrifugation liquid slowly, after dropwising completely, described mesh screen is taken out, then described density gradient centrifugation pipe is directly gone centrifugally to carry out subsequent operation.For containing the seminal fluid do not liquefied, then regulate the relative position of the filter of each layer screen pack further, thus make the aperture of each layer screen pack reduce gradually to reach separation requirement.
Claims (6)
1. the special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation, it is characterized in that, the top of described mesh screen is filter house (1), described filter house (1) cylindrically structure, the inside of described filter house is provided with screen pack, described screen pack is mutually superposed by the identical filter of two panels (10) and forms, and two panels filter (10) adjusts the change of relative position between the two by the aperture adjustment knob (11) be arranged on outside filter house; The bottom of described mesh screen is conduction part (6), the upper end of described conduction part (6) is connected with the bottom of described filter house (1), the opening of the lower end of described conduction part (6) reduces to side gradually and forms a draining hole (7) in bottom, and the below of described draining hole (7) is connected with drainage tube (8).
2. the special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation according to claim 1, it is characterized in that, the inside of described filter house (1) is provided with three layer filtration net from top to bottom, be respectively the first screen pack (2), the second screen pack (3) and the 3rd screen pack (4), the distance between every layer of screen pack is 1 ~ 5mm.
3. the special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation according to claim 2, it is characterized in that, the filter opening aperture of each filter of the first screen pack (2) is 30 ~ 35 μm, the filter opening aperture of each filter of the second screen pack (3) is 10 ~ 13 μm, and the filter opening aperture of each filter of the 3rd screen pack (4) is 6 ~ 9 μm.
4. the special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation according to claim 1, is characterized in that, described mesh screen has bracing frame (6) in the arranged outside of the junction of filter house (1) and conduction part (2).
5. the special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation according to claim 4, is characterized in that, described bracing frame (6) protrudes from the outside 1 ~ 2cm of described filter house (1).
6. the special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation according to claim 1, is characterized in that, the diameter of described drainage tube (8) is 2 ~ 5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420591885.2U CN204219887U (en) | 2014-10-13 | 2014-10-13 | A kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420591885.2U CN204219887U (en) | 2014-10-13 | 2014-10-13 | A kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation |
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| CN204219887U true CN204219887U (en) | 2015-03-25 |
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| CN201420591885.2U Expired - Fee Related CN204219887U (en) | 2014-10-13 | 2014-10-13 | A kind of special mesh screen for abnormal liquefaction seminal fluid density gradient centrifugation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108717111A (en) * | 2018-04-24 | 2018-10-30 | 金华市人民医院 | Full-automatic sperm detection device and method |
| CN114991189A (en) * | 2022-06-07 | 2022-09-02 | 广东耀南建设集团有限公司 | Deep basal pit of intelligence integration drainage system that falls |
-
2014
- 2014-10-13 CN CN201420591885.2U patent/CN204219887U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108717111A (en) * | 2018-04-24 | 2018-10-30 | 金华市人民医院 | Full-automatic sperm detection device and method |
| CN108717111B (en) * | 2018-04-24 | 2023-11-10 | 金华市人民医院 | Full-automatic semen detection device and method |
| CN114991189A (en) * | 2022-06-07 | 2022-09-02 | 广东耀南建设集团有限公司 | Deep basal pit of intelligence integration drainage system that falls |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150325 Termination date: 20191013 |