CN207775232U - Excretion body quick-speed extraction apparatus in a kind of cells and supernatant - Google Patents
Excretion body quick-speed extraction apparatus in a kind of cells and supernatant Download PDFInfo
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- CN207775232U CN207775232U CN201721879262.5U CN201721879262U CN207775232U CN 207775232 U CN207775232 U CN 207775232U CN 201721879262 U CN201721879262 U CN 201721879262U CN 207775232 U CN207775232 U CN 207775232U
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- pipe shaft
- fixed block
- pipe
- cells
- excretion body
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Abstract
The utility model discloses excretion body quick-speed extraction apparatus in a kind of cells and supernatant, including pipe lid and bottom portion fixed block, it is connected with the first pipe shaft below the pipe lid, and first is provided with the second pipe shaft below pipe shaft, it is provided with third pipe shaft below second pipe shaft, and the 4th pipe shaft is connected with below third pipe shaft, the middle inside of first pipe shaft is provided with primary filtration network, it is provided with exquisite filter screen below the middle rank filter screen, it is provided with side fixed block above the bottom fixed block, the inner upper of second pipe shaft is equipped with screw thread mouth, and second the lower inside of pipe shaft be provided with threaded post, gasket is installed on the outside of the threaded post.Excretion body quick-speed extraction apparatus is provided with side fixed block and bottom fixed block in the cells and supernatant, and side fixed block can remind the twisting resistance of operator's equipment during tightening, and preventing from overexerting leads to the rupture of the equipment, increases the practicability of equipment.
Description
Technical field
The utility model is related to excretion body extractor technical field, excretion body is quick in specially a kind of cells and supernatant
Extractor.
Background technology
Excretion body extractor is a kind of equipment for extracting excretion body in supernatant, and existing excretion body extractor is due to being all one
Structure needs to use multiple excretion body extractors, causes inconvenient problem during operation, and extraction efficiency is not high,
And extract when be easy influence excretion body bioactivity, and place when be susceptible to shaking the phenomenon that.
Therefore we have devised a kind of clothes automatic shearing bits-suction device, in order to solve it is above-mentioned proposed in ask
Topic.
Utility model content
It is above-mentioned to solve the purpose of this utility model is to provide excretion body quick-speed extraction apparatus in a kind of cells and supernatant
The excretion body extractor currently on the market that background technology proposes is inconvenient, and extraction efficiency is not high and is easy to influence excretion body
The problem of bioactivity.
To achieve the above object, the utility model provides the following technical solutions：Excretion body is fast in a kind of cells and supernatant
Fast extractor, including pipe lid and bottom portion fixed block are connected with the first pipe shaft below the pipe lid, and are set below the first pipe shaft
It is equipped with the second pipe shaft, third pipe shaft is provided with below second pipe shaft, and be connected with the 4th pipe shaft below third pipe shaft,
The middle inside of first pipe shaft is provided with primary filtration network, and intermediate filter screen is equipped with below primary filtration network, institute
It states and is provided with exquisite filter screen below intermediate filter screen, be provided with side fixed block above the bottom fixed block, described
The inner upper of two pipe shafts is equipped with screw thread mouth, and the lower inside of the second pipe shaft is provided with threaded post, the threaded post it is outer
Side is equipped with gasket.
Preferably, the pipe lid is threadedly coupled with the first pipe shaft composition, and pipe exterior surface is in wave structure.
Preferably, first pipe shaft, the second pipe shaft, third pipe shaft and the 4th pipe shaft constitute tube body, and between four
To be threadedly coupled, four be hollow structure, and the first pipe shaft, the second pipe shaft, third pipe shaft three's shape size are consistent, and
And the 4th pipe shaft bottom be in conical structure.
Preferably, the mesh diameter of the primary filtration network is 0.22um, and the mesh diameter of intermediate filter screen is 100nm,
The mesh diameter of exquisite filter screen is 40nm, and three constitutes filtration system, and the center line of three is parallel to each other.
Preferably, the side fixed block is provided with eight, and 8 side fixed blocks are divided into four groups, and opposite side fixed block closes
It is symmetrical in the center line of the 4th pipe shaft, and 8 side fixed blocks are in the same plane.
Preferably, the bottom fixed block is in " ten " word structure, and bottom fixed block is symmetrical about the center line of threaded post,
And the outer diameter of threaded post matches with the internal diameter of screw thread mouth.
Compared with prior art, the utility model has the beneficial effects that：Excretion body rapid extraction in the cells and supernatant
Device,
It is provided with the first pipe shaft, the second pipe shaft, third pipe shaft and the 4th pipe shaft, using sectional design, convenient for using
When dismounting to the equipment, increase the practicability of equipment；
Filter screen there are three types of different size is set, the equipment extraction efficiency is increased；
It is provided with side fixed block and bottom fixed block, and side fixed block can remind operator's equipment during tightening
Twisting resistance, preventing from overexerting leads to the rupture of the equipment, increases the practicability of equipment, and side fixed block and bottom fixed block
The phenomenon that improving the stablizing effect of equipment, coordinating the 4th pipe shaft bottom conical structure, prevent equipment from falling.
Description of the drawings
Fig. 1 is the utility model positive structure diagram；
Fig. 2 is the new second pipe shaft dimensional structure diagram of this practicality；
Fig. 3 is the utility model present invention looks up structural representation.
In figure：1, Guan Gai, the 2, first pipe shaft, the 3, second pipe shaft, 4, third pipe shaft, the 5, the 4th pipe shaft, 6, primary filtration network,
7, intermediate filter screen, 8, exquisite filter screen, 9, side fixed block, 10, gasket, 11, bottom fixed block, 12, screw thread mouth, 13, spiral shell
Line column.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
- 3 are please referred to Fig.1, the utility model provides a kind of technical solution：Excretion body quickly carries in a kind of cells and supernatant
Take device, including pipe lid 1, the first pipe shaft 2, the second pipe shaft 3, third pipe shaft 4, the 4th pipe shaft 5, primary filtration network 6, intermediate filter screen
7, exquisite filter screen 8, side fixed block 9, gasket 10, bottom fixed block 11, screw thread mouth 12 and threaded post 13, the lower section of pipe lid 1
It is connected with the first pipe shaft 2, and the lower section of the first pipe shaft 2 is provided with the second pipe shaft 3, pipe lid 1 and the first pipe shaft 2 constitute screw thread and connect
It connects, and 1 outer surface of pipe lid is in wave structure, is convenient for the dismounting of pipe lid 1 and the first pipe shaft 2, the first pipe shaft 2, the second pipe shaft 3, the
Three pipe shafts 4 and the 4th pipe shaft 5 constitute tube body, and are to be threadedly coupled between four, and four be hollow structure, and first manages
Body 2, the second pipe shaft 3,4 three's shape size of third pipe shaft are consistent, and 5 bottom of the 4th pipe shaft is in conical structure, and increase is set
Standby versatility, and 5 bottom of the 4th pipe shaft is in conical structure, is convenient for the placement of equipment, and the lower section of the second pipe shaft 3 is provided with the
Three pipe shafts 4, and the lower section of third pipe shaft 4 is connected with the 4th pipe shaft 5, the middle inside of the first pipe shaft 2 is provided with primary filtration network
6, and the lower section of primary filtration network 6 is equipped with intermediate filter screen 7, the mesh diameter of primary filtration network 6 is 0.22um, middle rank filtering
The mesh diameter of net 7 is 100nm, and the mesh diameter of exquisite filter screen 8 is 40nm, and three constitutes filtration system, and three
Center line it is parallel to each other, improve the practicability of the equipment, the lower section of intermediate filter screen 7 is provided with exquisite filter screen 8, and bottom is solid
The top for determining block 11 is provided with side fixed block 9, and side fixed block 9 is provided with eight, and 8 side fixed blocks 9 are divided into four groups, opposite
Side fixed block 9 is symmetrical about the center line of the 4th pipe shaft 5, and 8 side fixed blocks are in the same plane, convenient for consolidating for equipment
Fixed, bottom fixed block 11 is in " ten " word structure, and bottom fixed block 11 is symmetrical about the center line of threaded post 13, and threaded post
13 outer diameter matches with the internal diameter of screw thread mouth 12, and the dismounting of equipment, the inner upper of the second pipe shaft 3 is convenient for be equipped with screw thread mouth
12, and the lower inside of the second pipe shaft 3 is provided with threaded post 13, the outside of threaded post 13 is equipped with gasket 10.
Operation principle：When using excretion body quick-speed extraction apparatus in the cells and supernatant, first, by the first of the equipment
Pipe shaft 2, the second pipe shaft 3, third pipe shaft 4 and the 4th pipe shaft 5 are assembled, then the pipe lid of the equipment in clean bench
1 opens, then pipe shaft is put on rack for test tube, and equipment is fixed by side fixed block 9 and bottom fixed block 11, increases equipment
Stability, then collect cell culture supernatant 3000g, 10 minutes centrifugal operations are carried out in centrifuge, when completion from
After heart operation, supernatant is taken out, removes precipitation, then cell culture supernatant is transferred to the inside of the equipment, the equipment is each
Separable extraction cell culture supernatant is less than or equal to 15ml, and the pipe lid 1 of the equipment and the first pipe shaft 2 are tightened 3000g, and 15
The centrifugal operation of minute, after centrifugation, unscrews threaded post 13 and screw thread mouth 12 respectively, and 1mlPBS is drawn repeatedly with sterile 1ml pipette tips
The primary filtration network 6 inside the first pipe shaft 2 is purged, then discards suspension, then 1mlPBS is drawn with the sterile 1ml pipette tips of another
The intermediate filter screen 7 inside the second pipe shaft 3 is purged repeatedly, and the suspension containing excretion body is put into excretion body receiving flask, then is used
The sterile 1ml pipette tips of another draw 1mlPBS and purge the exquisite filter screen 8 inside third pipe shaft 4 repeatedly, will contain excretion body
Suspension is put into another excretion body receiving flask, if the volume of supernatant is more than 15ml, needs the pipe shaft the equipment
It is assembled, is repeated the above process again, until extracting the content successfully, not being described in detail in this specification belongs to this field
The prior art well known to professional technician.
Although the utility model is described in detail with reference to the foregoing embodiments, those skilled in the art is come
Say, still can with technical scheme described in the above embodiments is modified, or to which part technical characteristic into
Row equivalent replacement, within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on should all
It is included within the scope of protection of this utility model.
Claims (6)
1. excretion body quick-speed extraction apparatus in a kind of cells and supernatant, including pipe lid（1）With bottom fixed block（11）, feature exists
In：The pipe lid（1）Lower section be connected with the first pipe shaft（2）, and the first pipe shaft（2）Lower section be provided with the second pipe shaft（3）, institute
State the second pipe shaft（3）Lower section be provided with third pipe shaft（4）, and third pipe shaft（4）Lower section be connected with the 4th pipe shaft（5）, institute
State the first pipe shaft（2）Middle inside be provided with primary filtration network（6）, and primary filtration network（6）Lower section intermediate mistake is installed
Strainer（7）, the middle rank filter screen（7）Lower section be provided with exquisite filter screen（8）, the bottom fixed block（11）Top set
It is equipped with side fixed block（9）, second pipe shaft（3）Inner upper screw thread mouth is installed（12）, and the second pipe shaft（3）Inside
Lower section is provided with threaded post（13）, the threaded post（13）Outside gasket is installed（10）.
2. excretion body quick-speed extraction apparatus in a kind of cells and supernatant according to claim 1, it is characterised in that：The pipe
Lid（1）With the first pipe shaft（2）It constitutes and is threadedly coupled, and pipe lid（1）Outer surface is in wave structure.
3. excretion body quick-speed extraction apparatus in a kind of cells and supernatant according to claim 1, it is characterised in that：Described
One pipe shaft（2）, the second pipe shaft（3）, third pipe shaft（4）With the 4th pipe shaft（5）Tube body is constituted, and is that screw thread connects between four
It connects, four be hollow structure, and the first pipe shaft（2）, the second pipe shaft（3）, third pipe shaft（4）Three's shape size is consistent,
And the 4th pipe shaft（5）Bottom is in conical structure.
4. excretion body quick-speed extraction apparatus in a kind of cells and supernatant according to claim 1, it is characterised in that：It is described first
Grade filter screen（6）Mesh diameter be 0.22um, intermediate filter screen（7）Mesh diameter be 100nm, exquisite filter screen（8）'s
Mesh diameter is 40nm, and three constitutes filtration system, and the center line of three is parallel to each other.
5. excretion body quick-speed extraction apparatus in a kind of cells and supernatant according to claim 1, it is characterised in that：The side
Fixed block（9）It is provided with eight, and 8 side fixed blocks（9）It is divided into four groups, opposite side fixed block（9）About the 4th pipe shaft（5）
Center line it is symmetrical, and 8 side fixed blocks are in the same plane.
6. excretion body quick-speed extraction apparatus in a kind of cells and supernatant according to claim 1, it is characterised in that：The bottom
Portion's fixed block（11）In " ten " word structure, and bottom fixed block（11）About threaded post（13）Center line it is symmetrical, and screw thread
Column（13）Outer diameter and screw thread mouth（12）Internal diameter match.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721879262.5U CN207775232U (en) | 2017-12-28 | 2017-12-28 | Excretion body quick-speed extraction apparatus in a kind of cells and supernatant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721879262.5U CN207775232U (en) | 2017-12-28 | 2017-12-28 | Excretion body quick-speed extraction apparatus in a kind of cells and supernatant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207775232U true CN207775232U (en) | 2018-08-28 |
Family
ID=63225323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721879262.5U Active CN207775232U (en) | 2017-12-28 | 2017-12-28 | Excretion body quick-speed extraction apparatus in a kind of cells and supernatant |
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| Country | Link |
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| CN (1) | CN207775232U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363680A (en) * | 2018-12-26 | 2020-07-03 | 浙江大学 | Pulse stirring type bioreactor for secretion, separation and collection of exosome |
-
2017
- 2017-12-28 CN CN201721879262.5U patent/CN207775232U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363680A (en) * | 2018-12-26 | 2020-07-03 | 浙江大学 | Pulse stirring type bioreactor for secretion, separation and collection of exosome |
| CN111363680B (en) * | 2018-12-26 | 2023-10-13 | 浙江大学 | Pulse stirring type bioreactor for secretion, separation and collection of exosomes |
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Legal Events
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210104 Address after: Room 307, area C, 3 / F, building 1, 95 Lane 85, Cailun Road, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Patentee after: Shanghai hanheng Gene Technology Co.,Ltd. Address before: 201100 Shanghai Hengyuan Biotechnology Co., Ltd., room a148, building 1, 5500 Yuanjiang Road, Minhang District, Shanghai Patentee before: SHANGHAI HENGYUAN BIOTECHNOLOGY Co.,Ltd. |