CN203625369U

CN203625369U - Filter gun head of pipette for cell sphere experiment

Info

Publication number: CN203625369U
Application number: CN201320831825.9U
Authority: CN
Inventors: 朱廷准; 梁国标; 李晓明; 罗力涵
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-12-17
Filing date: 2013-12-17
Publication date: 2014-06-04
Anticipated expiration: 2023-12-17

Abstract

The utility model discloses a filter gun head of a pipette for a cell sphere experiment. The filter gun head comprises a suction tube (1), wherein the diameter of one end of the suction tube (1) is gradually reduced along the axial direction, and a gun head suction port (11) is formed in the end of the suction tube; a filter screen (2) is fixedly connected to the gun head suction port (11), and the aperture of the filter screen (2) is less than 50 microns. The filter gun head has the advantages of simple structure, reasonable design, convenience in use, good separating effect, low time consumption, low cost, simplicity in manufacturing and the like.

Description

A kind of pipettor for cell ball experiment filters rifle head

Technical field

The utility model relates to cell ball test tool, provides especially a kind of pipettor for cell ball experiment to filter rifle head.

Background technology

To the research of neural stem cell and tumor stem cell, become focus and the focus in current International Medical field.And above-mentioned two class cells all have the also characteristic of balling-up growth that suspends in special culture media, conventionally in the time that cell ball grows into 50 ~ 100 microns of diameters (approximately 60 ~ 100 cells), not only need it to carry out the various experiments such as immunofluorescence and detect, and need to keep the state of cell ball simultaneously.In experiment, usually need with all ingredients, cell ball to be suspended wherein, cell ball processed, this with regard to needs repeated multiple times by various liquid and cell ball draw separate after reject.

The method using at present is both at home and abroad generally centrifuging, that is: with whizzer by after cell ball centrifugation, use pipettor that supernatant liquid is absorbed, retain cell ball precipitation.But, due to the structural limitations of rifle head in existing pipettor, as easy as rolling off a log cell ball is sucked in rifle head, cause completing reagent and cell ball are carried out to effective object separating.In actually operating, the drawback of centrifuging mainly contains the following aspects:

(1) waste in a large number cell ball: in real work, find, centrifugal halfway situation usually occurs centrifuging.In supernatant liquor after each centrifugal, be still mixed with a small amount of cell ball and by reject, after repeated multiple times " centrifugal-to absorb " operation, can cause a large amount of loss of cell ball, cause subsequent experimental cell ball quantity not sufficient.Because the growth cycle of neural stem cell and tumor stem cell is grown (conventionally needing about 1 week), and special culture media (needing to add various kinds of cell somatomedin and nutritional additive etc.) is expensive, and centrifuging has also caused a large amount of wastes of reasearch funds.

(2) large to the damage of cell ball: in the time of the new reagent of each centrifugal rear interpolation, need to repeatedly to blow and beat throw out, so that cell ball is disconnected from each other and new reagent adding uniform contact, can make like this cell of cell ball periphery constantly come off, so that cell ball is more and more less, even the untight cell ball of some structure is dispelled, and loses original cell ball form.

(3) centrifugation step is loaded down with trivial details, consuming time: take the immunofluorescence experiment of cell ball as example, inhale centrifugal general needs while changing reagent 3 ~ 5 minutes at every turn, whole process need to through approximately 10 times centrifugal, only centrifugal consuming time just need to approximately 30 ~ 50 minutes.

Therefore, how the rifle head of pipettor is carried out to texture improvement, can realize and separating effective to reagent and cell ball, solve the variety of problems existing due to centrifuging, become people's problem demanding prompt solution.

Summary of the invention

Given this, the purpose of this utility model is to provide a kind of pipettor for cell ball experiment to filter rifle head, and to solve, the cost that centrifuging existed is in the past high, inferior separating effect, elapsed time are long and traditional pipettor gun head cannot be realized the problem that reagent is separated with cell ball.

The pipettor for cell ball experiment that the utility model provides filters rifle head, described filtration rifle head comprises suction pipe 1, successively decrease gradually and be provided with rifle head suction port 11 along its axial direction due diameter in described suction pipe 1 one end, it is characterized in that: on described rifle head suction port 11, be fixedly connected with filter screen 2, and the aperture of described filtering net 2 is less than 50 microns.

Preferably, the diameter of described rifle head suction port 11 is 1 millimeter.

Further preferably, the aperture of described filtering net 2 is 40 microns.

The pipettor for cell ball experiment that the utility model provides filters rifle head, by being provided with at rifle head suction port place the filtering net that aperture is less than cell ball diameter, realizes the object effectively reagent being separated with cell ball.

The pipettor for cell ball experiment that the utility model provides filters rifle head, can prevent on the one hand that cell ball from being sucked by rifle head with reagent and reject reduces the loss of cell ball; Can reduce repeatedly on the other hand centrifugal experimental implementation, this rifle head can make cell ball experiment more efficient, convenient, have simple in structure, reasonable in design, easy to use, good separating effect, elapsed time is short, cost is low, makes the advantages such as simple.

Accompanying drawing explanation

Below in conjunction with drawings and the embodiments, the utility model is described in further detail:

Fig. 1 is the structural representation that filters rifle head for the pipettor of cell ball experiment;

Fig. 2 is the use state graph of filtering rifle head for the pipettor of cell ball experiment;

Fig. 3 is the structural representation of filtering net.

Embodiment

Be illustrated in figure 1 a kind of pipettor for cell ball experiment and filter rifle head, filter rifle head and comprise suction pipe 1, wherein, successively decrease gradually and be provided with rifle head suction port 11 along its axial direction due diameter in suction pipe 1 one end, the other end is opening end, on rifle head suction port 11, be fixedly connected with and be useful on the filter screen 2 that cell ball is filtered out, and the aperture of filtering net 2 is to be less than 50 microns of cell ball diameter.

Wherein, Fig. 2 is the use state graph of this filtration rifle head, while using the mask work that it carries out reagent and cell ball, cell ball solution can be without centrifugal, only the suction pipe opening end of this filtration rifle head need be sleeved on to the front end of pipettor, then the rifle head suction port that filters rifle head is inserted in cell ball solution and directly absorbed, owing to being provided with at rifle head suction port the filtering net that aperture is less than cell ball diameter, can avoid cell ball to be inhaled into reject after rifle head with liquid, thereby reduce the loss of cell ball, again while adding reagent after effective minimizing is simultaneously each centrifugal, because repeatedly blowing and beating the damage that precipitation causes cell ball, thereby the spherical state of Cell protection is not destroyed, but also while greatly reducing experimental implementation because of the number of times of the required centrifugally operated of reject liquid, can obviously reduce experiment consuming time, increase work efficiency.

While as shown in Figure 1 the diameter of rifle head suction port 11 being designed to 1 millimeter, can improve rate of liquid aspiration, and can effectively avoid cell ball in absorption liquid process to block in the phenomenon of cell screen cloth, and in compounding practice process, softly rock rifle head, can make the operation of imbibition complete smoothly.

Because the diameter of general neural stem cell ball or tumor spheres is about 50 ~ 100 microns, the cell screen cloth of 40 micron pore size is fixed on to rifle head suction port place, can play and prevent that cell ball is inhaled into the effect in rifle head.

By reference to the accompanying drawings embodiment of the present utility model is described in detail above, but the utility model is not limited to above-mentioned embodiment, in the ken possessing those of ordinary skills, can also under the prerequisite that does not depart from the utility model aim, make various variations.

Claims

1. the pipettor for cell ball experiment filters rifle head, described filtration rifle head comprises suction pipe (1), successively decrease gradually and be provided with rifle head suction port (11) along its axial direction due diameter in described suction pipe (1) one end, it is characterized in that: on described rifle head suction port (11), be fixedly connected with filter screen (2), and the aperture of described filtering net (2) is less than 50 microns.

2. according to filtering rifle head for the pipettor of cell ball experiment described in claim 1, it is characterized in that: the diameter of described rifle head suction port (11) is 1 millimeter.

3. according to filtering rifle head for the pipettor of cell ball experiment described in claim 1, it is characterized in that: the aperture of described filtering net (2) is 40 microns.