CN210945589U

CN210945589U - Centrifugal sleeve for separating mixed spots

Info

Publication number: CN210945589U
Application number: CN201921935409.7U
Authority: CN
Inventors: 况林泽
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-11-11
Filing date: 2019-11-11
Publication date: 2020-07-07
Anticipated expiration: 2029-11-11

Abstract

The utility model relates to the field of laboratory separation equipment, in particular to a centrifugal sleeve for separating mixed spots, which comprises a filter and a digestion tube arranged on the upper side of the filter, the centrifugal sleeve further comprises a collecting tube, the digestion tube is detachably connected with the filter, a cell enrichment column is detachably connected inside the collecting tube, and the orifice of the cell enrichment column is detachably fixed on the filter; the filter comprises a filter body, a first drainage tank and a second drainage tank, wherein the middle part of a tube cavity of the filter is provided with a plurality of drainage holes, and the bottom of the tube cavity of the filter is paved with a first filtering membrane; and a second filtering membrane is arranged on the cell enrichment column. The experiment operation is simple, the time is saved, the loss of the sperm cells is reduced, the repeated digestion is avoided, and the loss of the sperm cells caused by improper operation in the digestive juice transfer tube and the washing process is avoided.

Description

Centrifugal sleeve for separating mixed spots

Technical Field

The utility model relates to an equipment field is used in the laboratory separation, specifically is a centrifugal sleeve pipe for separating mixed spot.

Background

At present, the forensic DNA inspection technology plays a crucial role in criminal judicial practice, can provide clues for case investigation and powerful material evidence for criminal case judgment. In sexual criminal cases, a spot formed by the mixing of male semen and female vaginal secretions and epithelial cells, known as a mixed spot, is often encountered. Often the mixed spot is presented on various types of carriers such as vaginal swab, underwear, paper towel, bed linen, etc., where the importance of vaginal swab as crime evidence is irreplaceable.

How to obtain single sperm cells from the mixed spots is the key for testing and identification, and the detection of STR type of DNA in the single sperm cells for individual identification is the key for case detection and litigation.

At present, most laboratories adopt a differential lysis method to separate mixed spots, but the method has the defects of long experimental time, complicated steps, insufficient differential lysis, high requirement on the operation technology of an identifying person and the like, and particularly, when a large number of female vaginal epithelial cells exist and the number of spermatids is small, the STR typing of complete and single spermatid DNA is difficult to obtain, and sometimes mixed STR typing or even STR typing of spermatid DNA cannot be detected. There are also many researches on mixed spot separation methods in forensic science, such as laser capture microdissection, immunomagnetic bead directed capture, microfluidic chip technology, etc. Although the methods can accurately capture target cells, the methods have the problems of complicated sample pretreatment, expensive equipment consumables, difficulty in basic popularization and the like.

Based on the above problem, the utility model provides a centrifugal sleeve pipe for separating mix spot.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a centrifugal sleeve pipe for separating mix spot to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

a centrifugal sleeve for separating mixed spots comprises a filter, a digestion tube arranged on the upper side of the filter, a collecting tube, a cell enriching column detachably connected in the collecting tube, and a tube opening of the cell enriching column detachably fixed on the filter; the filter comprises a filter, a first drainage tank, a first filtering membrane and a second filtering membrane, wherein the first drainage tank is provided with a plurality of drainage holes and is arranged in the middle of a tube cavity of the filter, and the first filtering membrane with the aperture of 5-40 mu m is paved at the bottom of the tube cavity of the filter; and a second filtering membrane is arranged on the cell enrichment column, and the aperture of the second filtering membrane is 0.2-5 um.

Assembling a digestion tube, a filter, a cell enrichment column and a collecting tube together, wherein a first drainage tank of a drainage hole on the filter blocks the carrier, and the carrier is removed by a primary centrifugation method; when the digestive juice passes through the first filtering membrane on the filter, the female vaginal epithelial cells and cell debris which are not completely digested in the first step of digestion are intercepted, and meanwhile, sperm cells are allowed to pass through; when the digestive juice passes through the second filtering membrane on the cell enriching column, sperm cells are intercepted, so that the sperm cells are enriched on the second filtering membrane, and finally the digestive juice is stored in the collecting pipe.

The experimental operation is simple, and a technician does not need to have a higher operation level; the time is saved, and the female vaginal epithelial cells which are not completely digested do not need to be repeatedly digested; the loss of the sperm cells is reduced, the loss of the sperm cells caused by repeated digestion and long-time digestion is avoided, and the loss of the sperm cells caused by improper operation in the digestive fluid transfer tube and the washing process can also be avoided; the proportion of male spermatids is obviously improved, the mixed STR typing is converted into single STR typing of male spermatids, and the single STR typing of female vaginal epithelial cells is converted into two mixed STR typing.

The utility model discloses further scheme: the aperture of the first filtering membrane is 5-40 um.

The utility model discloses further scheme again: the aperture of the first filtering membrane is 10 um.

The utility model discloses further scheme again: the aperture of the second filtering membrane is 0.45 um.

The utility model discloses further scheme again: the digestion tube and the collection tube are both EP tubes. The tube cover of the collecting tube can cover the tube opening of the collecting tube and can also cover the tube opening of the cell enrichment column.

The utility model discloses further scheme again: the first filtering membrane is made of a nylon mesh membrane, a PC (polycarbonate) membrane or a PE (polyethylene) membrane; and the material of the second filtering membrane is a nylon membrane.

The utility model discloses further scheme again: the digestion tube is detachably connected with the filter through threads; the collecting pipe and the cell enriching column are also detachably connected through threads; and the cell enrichment column and the filter are also detachably connected through threads.

The utility model discloses further scheme again: first clamping ring is laid to first filtration membrane upside, and first filtration membrane downside is installed and is seted up the second bleeder groove in a plurality of weeping holes.

The utility model discloses further scheme again: the third bleeder groove of seting up a plurality of weeping holes is installed to second filtration membrane downside, and is equipped with the filtration sieve between second filtration membrane and the third bleeder groove, and the second clamping ring of connection in cell enrichment post downside has been laid to second filtration membrane upside.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the experimental operation is simple, and a technician does not need to have a higher operation level;

2. the time is saved, and the female vaginal epithelial cells which are not completely digested do not need to be repeatedly digested;

3. the loss of the sperm cells is reduced, the loss of the sperm cells caused by repeated digestion and long-time digestion is avoided, and the loss of the sperm cells caused by improper operation in the digestive fluid transfer tube and the washing process can also be avoided;

4. obviously improving the proportion of male spermatids, converting the mixed STR typing into single STR typing of male spermatids, and converting the single STR typing of female vaginal epithelial cells into two mixed STR typing.

Drawings

Fig. 1 is a schematic structural diagram of a centrifugal sleeve for separating mixed spots according to the present invention.

Fig. 2 is a schematic sectional structure view of the filter of fig. 1.

In the figure:

1-a digestion tube; 2-a filter; 3-a cell enrichment column; 4-a collection tube;

31-a first drain tank; 32-a second drain tank;

41-a first filter membrane; 42-a first clamping ring;

71-a third drain tank; 72-a filter sieve plate; 73-a second filter membrane; 74-second clamping ring.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

Example 1

Referring to fig. 1 and 2, a centrifugal cannula for separating mixed spots includes a filter 2, and a digestion tube 1 disposed on the upper side of the filter 2, the centrifugal cannula further includes a collection tube 4, the digestion tube 1 is detachably connected with the filter 2, a cell enrichment column 3 is detachably connected inside the collection tube 4, and the orifice of the cell enrichment column 3 is detachably fixed on the filter 2;

the middle part of the tube cavity of the filter 2 is provided with a first liquid drainage tank 31 provided with a plurality of liquid drainage holes, and the bottom of the tube cavity of the filter 2 is paved with a first filtering membrane 41 with the aperture of 5-40 um;

the cell enrichment column 3 is provided with a second filtering membrane 73, and the aperture of the second filtering membrane 73 is 0.2-5 um.

Assembling a digestion tube 1, a filter 2, a cell enrichment column 3 and a collection tube 4 together, wherein a first drainage tank 31 of a drainage hole on the filter 2 blocks carriers, and carrying out primary centrifugation to remove the carriers; when the digestive juice passes through the first filtering membrane 41 on the filter 2, the female vaginal epithelial cells and cell debris which are not completely digested in the first step of digestion are retained, and meanwhile, the sperm cells pass through; when the digestive juice passes through the second filtering membrane 73 on the cell enriching column 3, sperm cells are intercepted, so that the sperm cells are enriched on the second filtering membrane 73, and finally the digestive juice is stored in the collecting tube 4.

Optimally, the pore diameter of the first filtering membrane 41 is 5-40 um; further, the pore diameter of the first filter membrane 41 is 10 um. That is, the pore size of the first filter membrane 41 is between the size of the sperm cell head and the size of the vaginal epithelium.

Preferably, the pore size of the second filter membrane 73 is 0.45 um. That is, the pore size of the second filter membrane 73 should be smaller than the smallest diameter of the sperm cell head.

Preferably, the digestion tube 1 and the collection tube 4 are both EP tubes. The tube cover of the collecting tube 4 can cover the tube opening of the collecting tube 4 and can also cover the tube opening of the cell enrichment column 3.

Preferably, the first filter membrane 41 is made of a nylon mesh membrane, a PC membrane or a PE membrane; and the second filter membrane 73 is made of a nylon membrane.

Example 2

As shown in fig. 1, the embodiment of the present invention is further defined based on embodiment 1.

Specifically, the digestion tube 1 and the filter 2 are detachably connected through threads; the collecting pipe 4 and the cell enrichment column 3 are also detachably connected through threads; and the cell-enriching column 3 and the filter 2 are also detachably connected by a screw.

A first pressing ring 42 is laid on the upper side of the first filtering membrane 41, and a second drainage tank 32 with a plurality of drainage holes is arranged on the lower side of the first filtering membrane 41.

The third drainage tank 71 provided with a plurality of drainage holes is installed on the lower side of the second filtering membrane 73, a filtering sieve plate 72 is arranged between the second filtering membrane 73 and the third drainage tank 71, a second pressing ring 74 connected to the lower side of the cell enrichment column 3 is paved on the upper side of the second filtering membrane 73, and under the condition of no centrifugal force, the second pressing ring 74 can ensure that liquid cannot flow into the collecting pipe 4.

The utility model discloses a theory of operation is: the utility model discloses a centrifugal sleeve pipe for separating mixed spot, when using, cut the carrier of taking the mixed spot of appropriate amount and put into alimentary canal 1, add appropriate amount digestive juice, cover the tube cover and digest for a certain time; then assembling the digestion tube 1, the filter 2, the cell enrichment column 3 and the collection tube 4 together, and placing the collection tube 4 into a centrifuge to centrifuge at 7000rpm for 2 minutes; wherein the first liquid drainage tank 31 of the liquid drainage hole on the filter 2 blocks the carrier, and the carrier is removed by a primary centrifugation method; when the digestive juice passes through the first filtering membrane 41 on the filter 2, the female vaginal epithelial cells and cell debris which are not completely digested in the first step of digestion are retained, and meanwhile, the sperm cells pass through; when the digestive juice passes through the second filtering membrane 73 on the cell enriching column 3, the sperm cells are intercepted, so that the sperm cells are enriched on the second filtering membrane 73, and finally the digestive juice is stored in the collecting tube 4; taking a proper amount of digestive juice for female DNA detection; the digestion tube 1 and the filter 2 are detached, the applicable digestion solution is added into the sperm cell enrichment column 3 to digest the sperm cells on the second filter membrane 73, the collection tube 4 is placed into a centrifuge to be centrifuged at 7000rpm for 2 minutes, and the digestion solution is used for male DNA detection.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A centrifugal sleeve for separating mixed spots comprises a filter (2) and a digestion tube (1) arranged on the upper side of the filter (2), and is characterized by further comprising a collecting tube (4), wherein the digestion tube (1) is detachably connected with the filter (2), a cell enrichment column (3) is detachably connected inside the collecting tube (4), and the orifice of the cell enrichment column (3) is detachably fixed on the filter (2); wherein the lumen mid-mounting of filter (2) has first bleeder (31) of seting up a plurality of effusion holes, and the first filtration membrane that the aperture is 5-40um has been laid to the lumen bottom of filter (2), be equipped with second filtration membrane on cell enrichment post (3), and the aperture of second filtration membrane (73) is 0.2-5 um.

2. A centrifugal cannula for separating mixed spots according to claim 1 wherein the pore size of the first filter membrane (41) is 10 um.

3. A centrifugal cannula for separating mixed spots according to claim 1 wherein the pore size of the second filter membrane (73) is 0.45 um.

4. A centrifugal cannula for separating mixed plaque according to claim 1 characterized by that the digestion tube (1) and the filter (2) are removably connected by screw thread; the collecting pipe (4) and the cell enrichment column (3) are also detachably connected through threads; and the cell enrichment column (3) and the filter (2) are also detachably connected through threads.

5. A centrifugal cannula for separating mixed plaques according to claim 1 or 4, wherein both the digestion tube (1) and the collection tube (4) are EP tubes.

6. The centrifugal cannula for separating mixed spots according to claim 1, wherein the material of the first filter membrane (41) is nylon mesh membrane or PC membrane or PE membrane; and the material of the second filtering membrane (73) is a nylon membrane.

7. The centrifugal sleeve for separating mixed spots according to claim 1, wherein a first pressing ring (42) is laid on the upper side of the first filtering membrane (41), and a second drainage groove (32) with a plurality of drainage holes is arranged on the lower side of the first filtering membrane (41).

8. The centrifugal sleeve for separating mixed spots according to claim 1, wherein a third drainage tank (71) provided with a plurality of drainage holes is installed on the lower side of the second filtering membrane (73), a filtering screen plate (72) is arranged between the second filtering membrane (73) and the third drainage tank (71), and a second pressing ring (74) connected to the lower side of the cell enrichment column (3) is paved on the upper side of the second filtering membrane (73).