CN215878360U

CN215878360U - Separator for gradient liquid separation

Info

Publication number: CN215878360U
Application number: CN202121022067.7U
Authority: CN
Inventors: 万杰
Original assignee: Chongqing Sichengrui Technology Co ltd
Current assignee: Chongqing Sichengrui Technology Co ltd
Priority date: 2021-05-13
Filing date: 2021-05-13
Publication date: 2022-02-22
Anticipated expiration: 2031-05-13

Abstract

The utility model belongs to the technical field of linear gradient liquid, in particular to a separator for separating gradient liquid; aiming at solving the problems of complicated gradient liquid separation method or high requirement on a test tube in the prior art; the separator comprises a pipe body with a tightened pipe orifice and a liquid discharge pipe arranged at the upper end of the pipe body; the lower edge of the pipe body is the same as the container for containing the gradient liquid in shape and size; when layered gradient liquid needs to be extracted, in the process that the hydraulic pipe is gradually pressed down, the upper-layer liquid flows out of the separator through the liquid discharge pipe, and the lower-layer liquid cannot be disturbed, so that the purity of the separated liquid is ensured; the utility model discloses the washing liquid is poured into to accessible scavenge pipe after using to discharge by the fluid-discharge tube after wasing the completion, accomplish the washing of fluid-discharge tube, the ball valve of setting between fluid-discharge tube and scavenge pipe then can avoid gradient liquid separation to take place by the scavenge pipe exhaust condition in the process.

Description

Separator for gradient liquid separation

Technical Field

The utility model relates to a linear gradient liquid technical field specifically is a separator for gradient liquid separation.

Background

Density gradient centrifugation is a separation means commonly used in laboratories for separating small molecules or smaller organelles, and the operation of the laboratories is generally that linear gradient liquid with a certain concentration range is prepared manually, then a sample needing centrifugal stratification is added slightly on the uppermost layer of the gradient liquid, and then the sample is centrifuged by an ultra-speed centrifuge. Because the gradient liquid with large concentration settles at the bottom of the test tube and the gradient liquid with small concentration settles at the upper part of the test tube, the sedimentation coefficient of the sample particles with large density is large, the downward sedimentation is fast, the sedimentation coefficient of the sample particles with small density is small, and the downward sedimentation is slow. Eventually, each sample particle will stay at the same density gradient position as it does not move down.

After centrifugation, the samples are layered, and the final key is how to take out the layered samples layer by layer, the upper layer and the lower layer of the gradient liquid are not disturbed, and the samples of each layer are separated out to ensure the purity of each layer. The methods mainly adopted at present comprise the following methods:

1. cutting method

Firstly putting the centrifuged centrifugal tube and the sample in the tube into a refrigerator for quick freezing, then cutting the frozen plastic tube into slices by using a centrifugal tube cutter, and taking out the samples layer by layer. This method is time consuming and many thick walled or metal tubes are not suitable.

2. Puncture method

The bottom of the centrifuge tube is punctured by a needle, so that the gradient freely drips out by gravity, and then the gradients are sequentially distributed and collected. The operation centrifuge tube can not be used, is not suitable for recovering gradient of sediment at the bottom of the tube, and is also not suitable for stainless steel or thick-wall plastic tubes.

3. Siphon method

A polyethylene vial was inserted into the bottom of the tube and the gradient was siphoned off, which caused the separation zone to be disrupted.

The above methods are complicated or have various requirements on the test tube in use, so that the development of experimental work is inconvenient.

SUMMERY OF THE UTILITY MODEL

The utility model provides a separator for gradient liquid separation has solved the loaded down with trivial details or problem that requires highly to the test tube of gradient liquid separation method among the prior art.

The technical scheme of the utility model is realized in this way, the utility model provides a separator for gradient liquid separation, the separator comprises a pipe body with a tightened pipe orifice and a liquid discharge pipe arranged at the upper end of the pipe body; the lower edge of the pipe body is the same as the container for containing the gradient liquid in shape and size.

Further, the body includes pressure liquid pipe, straight tube and connecting portion, the pressure liquid pipe is tubaeform hollow pipe, the pressure liquid pipe with the straight tube passes through connecting portion and connects detachablely, the through-hole of intercommunication pressure liquid pipe and straight tube is seted up to connecting portion.

Furthermore, a ball valve with the same radius as that of the through hole is arranged in the through hole of the connecting part.

Furthermore, the middle part of the connecting part is provided with a baffle plate with a hole, and the far ball valve side of the connecting part is provided with a cleaning pipe.

Further, the drain pipe is arranged on the side of the connecting part close to the ball valve.

Further, a sealing ring is arranged at the joint of the connecting part and the hydraulic pipe.

Furthermore, the middle part of the straight pipe is provided with an air duct, and two ends of the straight pipe are provided with connecting rods.

Further, the straight pipe, the connecting part and the hydraulic pipe are detachably connected through threads.

The utility model discloses a separator for gradient liquid separation has following beneficial effect:

1. the utility model discloses a separator for gradient liquid separation, when the gradient liquid of layering is extracted to needs, at the in-process that the hydraulic pressure pipe pushed down gradually, the upper strata liquid passes through the fluid-discharge tube and gushes out the separator, and lower floor's liquid then can not disturbed to guarantee the purity of separation liquid.

2. The utility model discloses the washing liquid is poured into to accessible scavenge pipe after using to discharge by the fluid-discharge tube after wasing the completion, accomplish the washing of fluid-discharge tube, the ball valve of setting between fluid-discharge tube and scavenge pipe then can avoid gradient liquid separation to take place by the scavenge pipe exhaust condition in the process.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic diagram of a separator for gradient liquid separation according to the present invention;

FIG. 2 is a cross-sectional view of the present application;

fig. 3 is a partially enlarged view of fig. 2.

In the figure: the device comprises a straight pipe 1, an air guide pipe 11, a connecting rod 12, a connecting part 2, a ball valve 21, a cleaning pipe 22, a liquid discharge pipe 23, a liquid pressing pipe 3 and a sealing ring 31.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by persons skilled in the art based on the embodiments in the present application without any creative work belong to the protection scope of the present application.

Examples

A separator for gradient liquid separation comprises a pipe body with a tightened pipe orifice and a liquid discharge pipe 23 arranged at the upper end of the pipe body; the lower limb of body and the shape of the splendid attire gradient liquid container, the size is the same, the body includes pressure liquid pipe 3, straight tube 1 and connecting portion 2, pressure liquid pipe 3 is tubaeform hollow pipe, pressure liquid pipe 3 passes through connecting portion 2 with the straight tube and is connected detachablely, connecting portion 2 has seted up the through-hole of intercommunication pressure liquid pipe 3 and straight tube 1, be equipped with radius and the same ball valve 21 of through-hole radius in the through-hole of connecting portion 2, connecting portion 2 middle part is equipped with foraminiferous baffle, 2 ball valve 21 side of far away of connecting portion 2 is seted up scavenge pipe 22, fluid-discharge tube 23 sets up in the nearly ball valve 21 side of connecting portion 2, connecting portion 2 is equipped with sealing washer 31 with pressure liquid pipe 3 junction, straight tube 1 middle part is equipped with air duct 11, and straight tube 1 both ends are equipped with connecting rod 12.

The embodiment has the beneficial effects through the following processes:

referring to fig. 2, this practicality is when the gradient liquid of layering needs to be extracted, and in the in-process that fluid-pressing pipe 3 pushed down gradually, upper strata liquid is squeezed out the separator through fluid-discharge tube 23, and lower floor's liquid then can not disturbed to guarantee the purity of separation liquid, and at the in-process of drawing, ball valve 21 is blocked by sealing washer 31 and can not fall, and after the upwelling of gradient liquid, ball valve 21 then is by jack-up, plays sealed effect, avoids gradient liquid to get into straight tube 1.

After use, a cleaning liquid can be injected through the cleaning pipe 22 to clean the connection part 2 and the pipe body, and discharged through the discharge pipe 23, and the cleaning liquid also plays a role of cleaning the discharge pipe 23 at the time of discharge.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. A separator for gradient liquid separation is characterized by comprising a pipe body with a tightened pipe orifice and a liquid discharge pipe arranged at the upper end of the pipe body; the lower edge of the pipe body is the same as the container for containing the gradient liquid in shape and size.

2. The separator for gradient liquid separation of claim 1, wherein the tube body comprises a pressure liquid tube, a straight tube and a connecting part, the pressure liquid tube is a trumpet-shaped hollow conduit, the pressure liquid tube is detachably connected with the straight tube through the connecting part, and the connecting part is provided with a through hole for communicating the pressure liquid tube and the straight tube.

3. The separator for gradient liquid separation of claim 2, wherein a ball valve having the same radius as the radius of the through hole is provided in the through hole of the connection part.

4. The separator for gradient liquid separation of claim 3, wherein the connecting part is provided with a baffle plate with a hole in the middle, and a cleaning pipe is arranged on the far ball valve side of the connecting part.

5. The separator for gradient liquid separation of claim 3, wherein the drain pipe is disposed on a ball valve side of the connection portion.

6. The separator for gradient liquid separation of claim 2, wherein a sealing ring is arranged at the joint of the connecting part and the hydraulic pipe.

7. The separator for gradient liquid separation of claim 2, wherein the straight tube is provided with a gas guide tube at the middle part and connecting rods at both ends.

8. The separator for gradient liquid separation of claim 2, wherein the straight tube, the connecting portion and the hydraulic tube are detachably connected by a screw thread.