CN216537164U - Single cell suspension filter equipment - Google Patents
Single cell suspension filter equipment Download PDFInfo
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- CN216537164U CN216537164U CN202123011289.1U CN202123011289U CN216537164U CN 216537164 U CN216537164 U CN 216537164U CN 202123011289 U CN202123011289 U CN 202123011289U CN 216537164 U CN216537164 U CN 216537164U
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- filter
- single cell
- cell suspension
- base part
- opening
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- 239000006285 cell suspension Substances 0.000 title claims abstract description 25
- 238000001914 filtration Methods 0.000 claims abstract description 23
- 239000003814 drug Substances 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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Abstract
The utility model relates to a single cell suspension filtering device, and belongs to the technical field of biological medicine experimental tools. Comprises a filter and a filter funnel; the filter is in a hollow cylinder shape, the upper end surface of the filter is provided with a first opening, and the lower end surface and the side surface of the filter are provided with filter screens; the filter funnel comprises a base part and a circulating part, wherein the base part is provided with a hollow inverted truncated cone-shaped cavity, and the circulating part is a hollow cone-shaped pipeline; the base part is communicated with the circulation part; a filter is arranged in the hollow inverted truncated cone-shaped cavity of the base part. The single cell suspension filter device is simple to install, convenient to filter and applicable to collecting pipes of various specifications, and the diameter of the filtering collecting pipe is variable.
Description
Technical Field
The utility model relates to a single cell suspension filtering device, and belongs to the technical field of biological medicine experimental tools.
Background
As single cell sequencing technologies mature, more and more research has focused on the single cell field. It is crucial how to obtain a suitable single cell suspension from biological tissue. The currently prevailing tissue digestion methods are enzymatic or combined mechanical and enzymatic methods, with more or less undigested tissue regardless of which method is chosen to digest the tissue; from animal tissue to single cell suspensions, there may be residual undigested tissue, which results in the need for separation during subsequent processing, separation of tissue and cells by using filters; most filters on the market are shown in fig. 1, and the main body is a filter screen. The filter 1 can only be matched with a 50ml centrifuge tube 2 due to large diameter, and cannot be matched with other centrifuge tubes with smaller diameter specifications. If the tissue is small and the cells are few, the waste is inevitably caused by using a large container for recovery; the filter 1 is placed in a 50ml centrifuge tube 2, and the leakage detection phenomenon is very serious when the filter cloth is blocked. Since the side wall of the filter is attached to the 50ml tube, the cell suspension will overflow from the joint of the outer side wall of the filter 1 and the inner side wall of the 50ml centrifuge tube 2, which will waste a large amount of specimen. Even without spillage, the filter needs to be shaken continuously by hand to allow the cell suspension to flow more smoothly into the collection tube. Therefore, this technical field needs to solve from practical how to obtain a simple installation, and it is convenient to filter, filters the changeable unicellular suspension filter equipment's of collecting pipe diameter size technical problem.
Disclosure of Invention
The utility model aims to solve the technical problem of how to obtain a single cell suspension filter device which is simple to install, convenient to filter and variable in diameter of a filter collecting pipe.
In order to solve the above problems, the technical scheme adopted by the utility model is to provide a single cell suspension filtering device, which comprises a filter and a filtering funnel; the filter is in a hollow cylinder shape, the upper end face of the filter is provided with a first opening, and the lower end face and the side face of the filter are provided with filter screens; the filter funnel comprises a base part and a circulating part, wherein the base part is provided with a hollow inverted truncated cone-shaped cavity, and the circulating part is a hollow cone-shaped pipeline; the base part is communicated with the circulation part; a filter is arranged in the hollow inverted truncated cone-shaped cavity of the base part.
Preferably, the upper end of the base part is provided with a circular second opening, and the diameter of the second opening is larger than that of the first opening on the upper end face of the filter.
Preferably, the lower end of the base part is provided with a circular lower bottom surface, and the center of the lower bottom surface is provided with a circular opening III; the third opening is communicated with the circulating part.
Preferably, the diameter of the lower bottom surface is larger than the diameter of the lower end surface of the filter.
Preferably, a gap is provided between a side surface of the filter and a side surface of the base portion.
Preferably, the height of the filter is equal to or less than the height of the base portion.
Preferably, the central axes of the base part and the flow part coincide.
Compared with the prior art, the utility model has the following beneficial effects:
the single cell suspension filter device which is simple to install, convenient to filter and variable in diameter of the filter collecting pipe is obtained; the device is simple and easy to install, and can be used as it is. Can be directly discarded after use. The size of the collection container can also be determined according to actual specific conditions; if the tissue is small and the cells are few, a small container is adopted for recycling, so that waste is avoided; otherwise, if the large tissues and cells are more, the large containers are used for recycling, so that the complex work of using a plurality of small containers for multiple times is avoided.
By applying the filter funnel, on one hand, the filter is fixed, and on the other hand, the filtering performance of the filter, namely 'four-way and eight-way', is fully exerted. The combined filter device is suitable for different adapters such as 15ml tubes or 50ml tubes.
Drawings
FIG. 1 is a schematic diagram of a filter and a 50ml centrifuge tube in the prior art.
FIG. 2 is a schematic diagram of a single cell suspension filtration device according to the present invention;
FIG. 3 is a schematic structural diagram of a single cell suspension filtration device according to the present invention;
FIG. 4 is a schematic structural diagram of a single cell suspension filtration device of the present invention;
reference numerals: 1. a filter; 2.50ml centrifuge tube; 3. filtering the mixture by a funnel; 4.15ml centrifuge tube.
Detailed Description
In order to make the utility model more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings:
as shown in FIGS. 1-4, the technical scheme adopted by the utility model is to provide a single cell suspension filtering device, which comprises a filter 1 and a filtering funnel 3; the filter 1 is in a hollow cylindrical shape, the upper end surface of the filter is provided with a first opening, and the lower end surface and the side surface of the filter are provided with filter screens; the filter funnel 3 comprises a base part and a circulating part, wherein the base part is provided with a hollow inverted truncated cone-shaped cavity, and the circulating part is a hollow cone-shaped pipeline; the base part is communicated with the circulation part; the hollow cavity of the base part with the inverted truncated cone shape is provided with a filter 1. The upper end of the base part is provided with a second round opening, and the diameter of the second opening is larger than that of the first opening on the upper end face of the filter 1. The lower end of the base part is provided with a circular lower bottom surface, and the center of the lower bottom surface is provided with a circular opening III; the third opening is communicated with the circulating part. The diameter of the bottom surface of the base part is larger than that of the lower end surface of the filter. A gap is provided between the side surface of the filter 1 and the side surface of the base portion. The height of the filter 1 is equal to or less than the height of the base portion. The central axes of the base part and the flow part are overlapped.
During filtration, collecting pipes with different specifications are determined to be adopted according to actual specific conditions, namely the size of tissues and the number of cells, if the tissues are small and the cells are few, a small container such as a 15ml centrifuge tube 4 in the figure 4 is adopted for recovery, so that waste is avoided; otherwise, if the large tissues and cells are more, the large containers are used for recycling, so that the complex work of using a plurality of small containers for multiple times is avoided.
The single cell suspension filter device which is simple to install, convenient to filter and variable in diameter of the filter collecting pipe is obtained; the device is simple and easy to install, and can be used as it is. Can be directly discarded after use. The size of the collection container can also be determined according to actual specific conditions; if the tissue is small and the cells are few, a small container is adopted for recycling, so that waste is avoided; otherwise, if the large tissues and cells are more, the large containers are used for recycling, so that the complex work of using a plurality of small containers for multiple times is avoided.
By applying the filter funnel, on one hand, the filter is fixed, and on the other hand, the filtering performance of the filter in a 'four-way and eight-reach' mode is fully exerted. The combined filter device is suitable for adapters with different specifications.
Be equipped with the clearance between the side of filter and the side of filtering funnel basilar part, avoided the outside overflow of the lateral wall of filter 1 and the inside wall junction cell suspension of 50ml centrifuging tube 2 that appear among the prior art, the condition of extravagant sample.
While the utility model has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the utility model. Those skilled in the art can make various changes, modifications and equivalent arrangements, which are equivalent to the embodiments of the present invention, without departing from the spirit and scope of the present invention, and which may be made by utilizing the techniques disclosed above; meanwhile, any changes, modifications and variations of the above-described embodiments, which are equivalent to those of the technical spirit of the present invention, are within the scope of the technical solution of the present invention.
Claims (7)
1. A single cell suspension filtering device is characterized by comprising a filter and a filtering funnel; the filter is in a hollow cylinder shape, the upper end face of the filter is provided with a first opening, and the lower end face and the side face of the filter are provided with filter screens; the filter funnel comprises a base part and a circulating part, wherein the base part is provided with a hollow inverted truncated cone-shaped cavity, and the circulating part is a hollow cone-shaped pipeline; the base part is communicated with the circulation part; a filter is arranged in the hollow inverted truncated cone-shaped cavity of the base part.
2. A single cell suspension filtration device according to claim 1 wherein: the upper end of basilar part is equipped with circular shape opening two, and the diameter of opening two is greater than the diameter of filter up end opening one.
3. A single cell suspension filtration device according to claim 2, wherein: the lower end of the base part is provided with a circular lower bottom surface, and the center of the lower bottom surface is provided with a circular opening III; the third opening is communicated with the circulating part.
4. A single cell suspension filtration device according to claim 3, wherein: the diameter of the lower bottom surface is larger than that of the lower end surface of the filter.
5. The single cell suspension filtration device of claim 4, wherein: a gap is arranged between the side face of the filter and the side face of the base part.
6. A single cell suspension filtration device according to claim 5, wherein: the height of the filter is less than or equal to that of the base part.
7. A single cell suspension filtration device according to claim 6, wherein: the central axis of the basal part and the central axis of the circulating part coincide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123011289.1U CN216537164U (en) | 2021-12-02 | 2021-12-02 | Single cell suspension filter equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123011289.1U CN216537164U (en) | 2021-12-02 | 2021-12-02 | Single cell suspension filter equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216537164U true CN216537164U (en) | 2022-05-17 |
Family
ID=81538180
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123011289.1U Expired - Fee Related CN216537164U (en) | 2021-12-02 | 2021-12-02 | Single cell suspension filter equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216537164U (en) |
-
2021
- 2021-12-02 CN CN202123011289.1U patent/CN216537164U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220517 |