CN220166204U - Culture flask convenient to stem cell exosome is collected - Google Patents
Culture flask convenient to stem cell exosome is collected Download PDFInfo
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- CN220166204U CN220166204U CN202320517027.2U CN202320517027U CN220166204U CN 220166204 U CN220166204 U CN 220166204U CN 202320517027 U CN202320517027 U CN 202320517027U CN 220166204 U CN220166204 U CN 220166204U
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- bottle body
- collecting
- culture
- stem cell
- collecting bottle
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 42
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 28
- 239000011148 porous material Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 abstract description 7
- 239000006228 supernatant Substances 0.000 description 5
- 210000004748 cultured cell Anatomy 0.000 description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229960003160 hyaluronic acid Drugs 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- -1 L-type Polymers 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229920002845 Poly(methacrylic acid) Polymers 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000035992 intercellular communication Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000001995 reticulocyte Anatomy 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a culture bottle convenient for collecting stem cell exosomes, which comprises a culture bottle body and a collecting bottle body, wherein the collecting bottle body is detachably arranged above the culture bottle body, a first filter screen is arranged at the top of the culture bottle body, a second filter screen is arranged at the top of the collecting bottle body, an exosome collecting ball is arranged in the collecting bottle body, and the culture bottle further comprises a bottle cap assembly, and the bottle cap assembly is respectively connected with the culture bottle body and the collecting bottle body; through setting up separable blake bottle body and collecting bottle body, stem cell exosome can cultivate in the blake bottle body, and the accessible collecting bottle body is direct to be collected after cultivateing ripely, need not to transfer the container many times and separates, has effectively improved collection efficiency, has avoided the pollution of collecting the in-process simultaneously, has improved collection quality.
Description
Technical Field
The utility model relates to the technical field of exosome collection, in particular to a culture bottle convenient for collecting stem cell exosomes.
Background
Exosomes refer to small vesicles containing complex RNAs and proteins, which were first found in sheep reticulocytes in 1983. A variety of cells secrete exosomes under normal and pathological conditions, and exosomes are naturally found in body fluids, including blood, saliva, urine, cerebrospinal fluid and milk. Exosomes are considered as specifically secreted vesicles that are involved in intercellular communication and thus are important for research of exosomes.
In the prior art, the exosome is collected by sucking the supernatant of the cultured cells and then moving the supernatant to a separation device for multiple separation, and the process is complicated and the efficiency is low.
Therefore, a need exists for a culture flask that facilitates the collection of stem cell exosomes, and that can solve the problems of complicated process and low efficiency caused by the need of extracting the supernatant of the cultured cells and then separating the supernatant multiple times in the conventional exosome collection.
Disclosure of Invention
The utility model aims to provide a culture bottle convenient for collecting stem cell exosomes, so as to solve the problems of complicated process and low efficiency caused by the fact that the supernatant of cultured cells is required to be sucked and separated for multiple times in the conventional exosome collection.
In order to achieve the above object, the present utility model provides the following solutions:
the utility model provides a culture bottle convenient for collecting stem cell exosomes, which comprises a culture bottle body and a collecting bottle body, wherein the collecting bottle body is detachably arranged above the culture bottle body, a first filter screen is arranged at the top of the culture bottle body, a second filter screen is arranged at the top of the collecting bottle body, an exosome collecting ball is arranged in the collecting bottle body, and the culture bottle further comprises a bottle cap assembly, and the bottle cap assembly is respectively connected with the culture bottle body and the collecting bottle body.
Preferably, the upper part of the culture bottle body is of a frustum-shaped structure, and the top of the culture bottle body is provided with a culture bottle body opening.
Preferably, the culture bottle body opening is connected with the first filter screen, and the culture bottle body opening is connected with the collecting bottle body through threads.
Preferably, the inner side wall of the culture bottle body is provided with a spiral bulge.
Preferably, the lower part of the collecting bottle body is of a frustum-shaped structure and is matched with the upper part of the culture bottle body, the bottom of the collecting bottle body is provided with a collecting bottle body lower opening, and the collecting bottle body lower opening is connected with the culture bottle body opening through threads.
Preferably, the upper part of the collecting bottle body is of a frustum-shaped structure, an upper opening of the collecting bottle body is arranged at the top of the collecting bottle body, and the upper opening of the collecting bottle body is connected with the second filter screen through threads.
Preferably, the bottle cap assembly comprises a cap body, wherein the cap body is connected with the opening of the culture bottle body and the upper opening of the collecting bottle body through threads, a guide pipe is arranged at the top of the cap body, and a sealing plug is arranged on the guide pipe.
Preferably, the pore size of the first filter screen is 100nm, and the pore size of the second filter screen is 30nm.
Compared with the prior art, the utility model has the following beneficial technical effects:
the utility model provides a culture bottle convenient for collecting stem cell exosomes, which comprises a culture bottle body and a collecting bottle body, wherein the collecting bottle body is detachably arranged above the culture bottle body, a first filter screen is arranged at the top of the culture bottle body, a second filter screen is arranged at the top of the collecting bottle body, an exosome collecting ball is arranged in the collecting bottle body, and the culture bottle further comprises a bottle cap assembly, and the bottle cap assembly is respectively connected with the culture bottle body and the collecting bottle body; through setting up separable blake bottle body and collecting bottle body, stem cell exosome can cultivate in the blake bottle body, and the accessible collecting bottle body is direct to be collected after cultivateing ripely, need not to transfer the container many times and separates, has effectively improved collection efficiency, has avoided the pollution of collecting the in-process simultaneously, has improved collection quality.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a culture flask for facilitating collection of stem cell exosomes according to the present utility model;
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The utility model aims to provide a culture bottle convenient for collecting stem cell exosomes so as to solve the problems in the prior art.
In order that the above-recited objects, features and advantages of the present utility model will become more readily apparent, a more particular description of the utility model will be rendered by reference to the appended drawings and appended detailed description.
Example 1:
this embodiment provides a blake bottle convenient to stem cell exosome is collected, as shown in fig. 1, including blake bottle body 1 and collecting bottle body 2, collecting bottle body 2 detachably sets up in the top of blake bottle body 1, and the top of blake bottle body 1 is equipped with first filter screen 3, and the top of collecting bottle body 2 is equipped with second filter screen 4, and the inside of collecting bottle body 2 is equipped with exosome collecting ball 5, still includes bottle lid subassembly 6, and bottle lid subassembly 6 cooperatees with blake bottle body 1 and collecting bottle body 2 respectively.
Specifically, the upper portion of culture bottle 1 is the frustum shape structure, and its top is equipped with culture bottle opening 7, and frustum shape structure can make things convenient for the discharge of stem cell in the culture bottle 1.
Further, the culture bottle opening 7 and the first filter screen 3 and the culture bottle opening 7 and the collecting bottle 2 are connected through threads, so that effective and tight connection is convenient.
Further, the inside wall of the culture bottle body 1 is provided with a spiral bulge, so that the area of the inside wall of the culture bottle body 1 can be effectively increased, the culture area of adherent cells is further increased, the culture quantity of stem cells is increased, and the concentration of secreted cytokines is improved.
Further, the lower part of the collecting bottle body 2 is of a frustum-shaped structure and is matched with the upper part of the culture bottle body 1, a collecting bottle body lower opening 8 is arranged at the bottom of the collecting bottle body 2, and the collecting bottle body lower opening 8 is connected with the culture bottle body opening 7 through threads.
Further, the upper portion of the collecting bottle body 2 is of a frustum-shaped structure, and in order to facilitate the discharge of the exosome collecting balls 5, an upper collecting bottle body opening 9 is formed in the top of the collecting bottle body, and the upper collecting bottle body opening 9 is connected with the second filter screen 4 through threads.
Further, the bottle cap assembly 6 comprises a cap body 61, the cap body 61 is connected with the culture bottle body opening 7 and the cap body 61 is connected with the collecting bottle body upper opening 9 through threads, a guide pipe 62 is arranged at the top of the cap body 61, and a sealing plug 63 is arranged on the guide pipe 62.
Further, the pore size of the first filter 3 is 100nm, and the pore size of the second filter 4 is 30nm.
The utility model provides a culture bottle convenient for collecting stem cell exosomes, which comprises the following using method: injecting culture solution into the culture bottle 1 through the culture bottle opening 7, inoculating required stem cells, wherein the stem cells can be embryonic stem cells, induced pluripotent stem cells, fat stem cells, nerve stem cells, mesenchymal stem cells, tumor stem cells and the like, sealing the culture bottle opening 7 by using the bottle cap assembly 6 after inoculation is finished, shaking the culture bottle 1 lightly, enabling the stem cells to be uniformly attached to spiral bulges on the inner side wall of the culture bottle 1, taking down the bottle cap assembly 6 after culture is finished, screwing the first filter screen 3 into the lower opening 8 of the collection bottle, screwing the second filter screen 4 into the upper opening 9 of the collection bottle, and finally sealing the upper opening 9 of the collection bottle by using the bottle cap assembly 6, wherein the pore diameter of the first filter screen 3 is 100nm, so that water and exosome in the culture solution can all enter the collection bottle 2 through the first filter screen 3, the exosome can be adhered to the exosome collection ball 5, and the exosome collection ball 5 are macromolecule (such as macromolecule, hyaluronic acid, L-type, hyaluronic acid, etc.) formed by chemical or physical cross-linking; the synthesized hydrophilic polymer comprises alcohol, acrylic acid and derivatives thereof (polyacrylic acid, polymethacrylic acid, polyacrylamide, poly-N-poly-acrylamide, polyvinyl alcohol and the like), at this time, the sealing plug 63 is opened, moisture in the culture solution can flow out of the guide pipe 62, and as the aperture of the second filter screen 4 is 30nm, the secretion does not penetrate through the second filter screen 4, the bottle cap assembly 6 is unscrewed after the moisture is discharged, and the exosome collecting ball 5 is discharged and subjected to the next operation.
The principles and embodiments of the present utility model have been described with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present utility model; also, it is within the scope of the present utility model to be modified by those of ordinary skill in the art in light of the present teachings. In summary, the present description should not be construed as limiting the utility model.
Claims (8)
1. Culture bottle convenient to stem cell exosome is collected, its characterized in that: including blake bottle body and collecting bottle body, collecting bottle body detachably set up in the top of blake bottle body, the top of blake bottle body is equipped with first filter screen, the top of collecting bottle body is equipped with the second filter screen, the inside of collecting bottle body is equipped with the exosome and collects the ball, still includes the bottle lid subassembly, the bottle lid subassembly respectively with the blake bottle body with the collecting bottle body.
2. The culture flask for facilitating collection of stem cell exosomes of claim 1, wherein: the upper part of the culture bottle body is of a frustum-shaped structure, and the top of the culture bottle body is provided with a culture bottle body opening.
3. The culture flask for facilitating collection of stem cell exosomes of claim 2, wherein: the culture bottle body opening is connected with the first filter screen, and the culture bottle body opening is connected with the collecting bottle body through threads.
4. A culture flask for facilitating collection of stem cell exosomes according to claim 3, characterized in that: the inside wall of the culture bottle body is provided with a spiral bulge.
5. The culture flask for facilitating collection of stem cell exosomes of claim 4, wherein: the lower part of the collecting bottle body is of a frustum-shaped structure and is matched with the upper part of the culture bottle body, the bottom of the collecting bottle body is provided with a collecting bottle body lower opening, and the collecting bottle body lower opening is connected with the culture bottle body opening through threads.
6. The culture flask for facilitating collection of stem cell exosomes of claim 5, wherein: the upper portion of the collecting bottle body is of a frustum-shaped structure, an upper opening of the collecting bottle body is formed in the top of the collecting bottle body, and the upper opening of the collecting bottle body is connected with the second filter screen through threads.
7. The culture flask for facilitating collection of stem cell exosomes of claim 6, wherein: the bottle cap assembly comprises a cap body, wherein the cap body is connected with the opening of the culture bottle body and the upper opening of the collecting bottle body through threads, a guide pipe is arranged at the top of the cap body, and a sealing plug is arranged on the guide pipe.
8. The culture flask for facilitating collection of stem cell exosomes according to any one of claims 1-7, wherein: the pore diameter of the first filter screen is 100nm, and the pore diameter of the second filter screen is 30nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320517027.2U CN220166204U (en) | 2023-03-16 | 2023-03-16 | Culture flask convenient to stem cell exosome is collected |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320517027.2U CN220166204U (en) | 2023-03-16 | 2023-03-16 | Culture flask convenient to stem cell exosome is collected |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220166204U true CN220166204U (en) | 2023-12-12 |
Family
ID=89053328
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320517027.2U Active CN220166204U (en) | 2023-03-16 | 2023-03-16 | Culture flask convenient to stem cell exosome is collected |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220166204U (en) |
-
2023
- 2023-03-16 CN CN202320517027.2U patent/CN220166204U/en active Active
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