CN208791633U - A kind of separation of high-quality sperm and extraction element - Google Patents
A kind of separation of high-quality sperm and extraction element Download PDFInfo
- Publication number
- CN208791633U CN208791633U CN201821251002.8U CN201821251002U CN208791633U CN 208791633 U CN208791633 U CN 208791633U CN 201821251002 U CN201821251002 U CN 201821251002U CN 208791633 U CN208791633 U CN 208791633U
- Authority
- CN
- China
- Prior art keywords
- tube
- deposition portion
- sperm
- separation
- extraction element
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Centrifugal Separators (AREA)
- External Artificial Organs (AREA)
Abstract
The utility model discloses a kind of separation of high-quality sperm and extraction elements.To overcome existing separation and extraction element, suction pipe is easy the defect polluted by sperm inferior during the extraction process, the technical solution adopted in the utility model includes centrifuge tube and suction pipe, centrifuge tube includes the tube body of upper end opening and covers the pipe lid at body open, wherein, the tube body includes the pipe shaft of straight wall and the tube bottom of back taper, the bottom of tube bottom rides out to form deposition portion downwards, and the deposition portion is in U-shape；The outer diameter of the suction nozzle of the suction pipe is less than the internal diameter in the deposition portion, draws sperm so that suction nozzle protrudes into deposition portion.Its advantage is that: the case where suction pipe is polluted by sperm inferior can be efficiently reduced, the probability that suction pipe extracts high-quality sperm is improved.
Description
Technical field
The utility model belongs to field of medical device, and in particular to a kind of separation of high-quality sperm and extraction element.
Background technique
In recent years, with the gradually postponement of childbearing age, the disease incidence of infertility is risen year by year.Insémination intrautérine
It (IUI) is reproductive center as the prefered method for treating slight dysspermatism or unknown sterility.The success rate of IUI is by a variety of
The influence of factor: including clinical factor, such as seminal parameters, female age；Technical factor, such as IUI conduit type, fertilization time
Deng.Wherein liquefacient duration is the important link of IUI.
Liquefacient duration, the separation and extraction of also referred to as high-quality sperm, in particular to: according to manually operated alternative reduce or remove
The substances such as Necrospermia, active oxygen in sperm obtain sperm high-quality and with fertility, to increase sperm and egg cell
Rate of fertilization improves male sterility status, and then improves pregnancy rate.
Currently, the separation and extraction of high-quality sperm mostly use greatly centrifuge tube that discontinuous density gradient method is cooperated to complete.Density
Gradient media is made of the relatively high substance of molecular weight, and the characteristic with Hyposmolality, when preparing liquefacient duration culture solution
Need to reach osmotic pressure identical with female genital tract juice.Density gradient media and sperm to be processed are successively added to
In centrifuge tube, centrifugally operated is carried out.Due to other cell components in high-quality sperm and defective sperm, vigor difference sperm and sperm
Locomitivity, motion profile and in terms of there is difference, after centrifugation, the various composition in sperm is in density gradient
Reach balance in solution column, rests on respective equal buoyant densities point, and top-quality sperm, that is, high-quality sperm,
The bottom can be rested on.It reuses matched suction pipe to draw, the extraction of high-quality sperm can be completed.
But this separation and extraction element existing defects in actual use: high-quality sperm layer and sperm layer inferior it
Between there are interfaces, when the head of suction pipe is inserted into centrifuge tube, the interface will necessarily be stirred, cause high-quality sperm with it is bad
Matter sperm mixes once again, moreover, interface area is consistent with the cross-sectional area of the position centrifuge tube, and the cross-sectional area of centrifuge tube
Usually larger, what suction pipe insertion process bring interface was stirred involve, and range is big, and the suction nozzle of suction pipe is polluted by sperm inferior
A possibility that it is big, thus can doped with sperm inferior in the high-quality sperm extracted.Also having part operation is to complete centrifugation step
After rapid, first upper layer mixed solution is sopped up, it is still, this complex for operation step, and the upper layer mixed solution with sperm inferior
It not can guarantee and all suck, the contaminated probability of the suction nozzle of suction pipe is still high yet.
Summary of the invention
The utility model provides separation and the extraction element of a kind of high-quality sperm, can be improved suction pipe and extracts high-quality sperm
Probability.
In order to solve the above technical problems, the utility model adopts the following technical solution: a kind of separation of high-quality sperm and mentioning
Device, including centrifuge tube and suction pipe are taken, centrifuge tube includes the tube body of upper end opening and covers the pipe lid at body open, wherein
The tube body includes the pipe shaft of straight wall and the tube bottom of back taper, and the bottom of tube bottom rides out to form deposition portion downwards, the deposition portion
It is in U-shape；The outer diameter of the suction nozzle of the suction pipe is less than the internal diameter in the deposition portion, draws sperm so that suction nozzle protrudes into deposition portion.
The biggish pipe shaft of cross-sectional area can be gradually reduced from top to bottom for the tube bottom of back taper, make the cross section in deposition portion
Product is small.Density gradient media and sperm to be processed are successively added in centrifuge tube, after carrying out centrifugally operated, high-quality sperm energy
It is enough accumulated in deposition portion by density gradient media, concentrates position of the high-quality sperm in centrifuge tube more, convenient for extracting.
Range that bring interface was stirred moreover, the cross-sectional area in deposition portion is small, when the suction nozzle insertion of suction pipe involve is small, high-quality essence
A possibility that son is mixed with sperm inferior is small, ensure that the quality that high-quality sperm extracts.In addition, deposition portion from the bottom of tube bottom to
Under ride out to be formed, that is to say, that be it is with tube body integrally formed, it is easy to process, it is at low cost.
As an improvement, the deposition portion and the pipe shaft are coaxially disposed.This setting, so that when centrifugally operated, high-quality essence
Son can more successfully reach deposition portion, and the suction nozzle for being also convenient for suction pipe is inserted into deposition portion, in addition, the structural strength of tube body
It is higher.
Preferably, the length in the deposition portion is 3mm~12mm；The internal diameter in the deposition portion is 2mm~6mm.Deposition
The moderate length in portion, not only can guarantee the capacity in deposition portion, but also be avoided that structural strength is low caused by deposition portion is too long, it is broken to be easy
The problem of damage；The internal diameter in deposition portion is moderate, both can guarantee the capacity in deposition portion, is also convenient for the suction nozzle insertion of suction pipe, inhales to suction pipe
The size of head requires low, moreover it is possible to avoid because deposition portion it is excessive caused by cross-sectional area is excessive, interface size is big problem, protect
High-quality sperm is demonstrate,proved to be extracted.
As an improvement, the separation and the isolated tube that extraction element includes hollow and upper and lower ends are open, isolated tube position
In in centrifuge tube, the lower end of isolated tube extends to tube bottom and lower port is connected to tube bottom, and the lower port of the isolated tube is equipped with downward
The convex ribs of extension, the convex ribs extend into deposition portion and are interference fitted with the inner wall in deposition portion, and the internal diameter of the isolated tube is big
In the outer diameter of the suction nozzle of the suction pipe, so that suction nozzle is inserted into isolated tube.
Convex ribs and deposition portion are interference fitted, and are stood in centrifuge tube so that isolated tube is able to be supported, isolated tube and centrifugation
The structure of pipe is all simple, and the assembly, fractionation of the two also facilitate.
The use step of separation and extraction element with isolated tube are as follows: isolated tube is mounted in centrifuge tube；It pours into close
Gradient media is spent, since the lower port of isolated tube is connected to tube bottom, density gradient media can be between centrifuge tube and isolated tube
Flowing, keeps unified liquid level；, centrifuge tube in (isolation pipe outer wall and the space that is centrifuged inside pipe wall formation) injection outer toward isolated tube
Sperm to be processed, due to the setting of isolated tube, before centrifugally operated, sperm will not be entered inside isolated tube；It is centrifuged
Operation, high-quality sperm can reach tube bottom by density gradient media, and it is most of concentrate the deposition portion that accumulates in, and sperm inferior
It then rests on the outside of upper layer, that is, isolated tube, can not enter in isolated tube via the bottom of isolated tube；The suction nozzle of suction pipe is inserted into
Isolated tube, and the lower port for passing through isolated tube reaches deposition portion, extracts high-quality sperm.In this process, the suction nozzle of suction pipe only connects
Contact the density gradient media in isolated tube, and without poor quality sperm in density gradient media herein, it is heavy then directly to reach
Product portion and high-quality sperm contact avoid the problem of being polluted in extraction process by sperm inferior, improve and extract high-quality sperm
Success rate and probability.
In addition, the setting of isolated tube can also play the role of guidance to the insertion of suction pipe, so that extraction operation is more just
Benefit.
As an improvement, the convex ribs there are two, it is separately positioned on the opposite sides of the lower port of isolated tube.The knot of isolated tube
Structure is simple, and symmetrically arranged convex ribs can better play supporting role, make position of the isolated tube in centrifuge tube it is stable, can
It leans on.
As an improvement, the width of the convex ribs is gradually reduced from top to bottom.The convex ribs that width is gradually reduced from top to bottom are more
It is easily deformable, so that isolated tube is inserted into deposition portion more quickly.
As an improvement, the caliber of the isolated tube is gradually reduced from top to bottom.This setting, one come be convenient for isolated tube at
Type, demoulding, two are convenient for the suction nozzle of suction pipe to be inserted into.
Detailed description of the invention
Fig. 1 is the explosive view of the utility model；
Fig. 2 is the installation diagram of the utility model；
Fig. 3 is the schematic diagram of another angle of isolated tube of the utility model；
Fig. 4 is schematic diagram when the utility model is used (isolated tube is attached in centrifuge tube and density gradient media is added)；
Fig. 5 is schematic diagram (sperm to be processed is injected on the basis of Fig. 4) when the utility model is used；
Fig. 6 is schematic diagram (centrifugally operated is carried out on the basis of Fig. 5) when the utility model is used；
It is as shown in the figure: 1, centrifuge tube；11, Guan Gai；12, pipe shaft；13, tube bottom；14, deposition portion；2, isolated tube；21, convex ribs；
3, suction pipe；4, density gradient media；5, sperm.
Specific embodiment
Below with reference to the attached drawing of the utility model embodiment, the technical solution of the utility model embodiment is explained and
Illustrate, but following embodiments are only the preferred embodiment of the utility model, and not all.Based on the implementation example in the implementation mode,
Those skilled in the art's other embodiments obtained without making creative work, belong to the utility model
Protection scope.
A kind of separation of high-quality sperm as shown in Figure 1 and extraction element, including centrifuge tube 1, isolated tube 2 and suction pipe 3.
The centrifuge tube 1 includes the tube body of upper end opening and covers the pipe lid 11 at body open.The tube body includes straight
The pipe shaft 12 of wall and the tube bottom 13 of back taper, the bottom of tube bottom 13 ride out to form deposition portion 14 downwards, and the deposition portion 14 is in U
Shape.The deposition portion 14 is coaxially disposed with the pipe shaft 12.
The length in the deposition portion 14 is 3mm~12mm；The internal diameter in the deposition portion 14 is 2mm~6mm.Specifically in this example
In, the length in the deposition portion 14 is 7.2mm, and the internal diameter in the deposition portion 14 is 3.3mm.
As shown in Figure 2 and Figure 3, the isolated tube 2 is hollow, and upper and lower ends are open.Isolated tube 2 is located at centrifuge tube 1
Interior, the lower end of isolated tube 2 extends to tube bottom 13 and lower port is connected to tube bottom 13.The lower port of the isolated tube 2 is equipped with to downward
The convex ribs 21 stretched, the convex ribs 21 extend into deposition portion 14 and are interference fitted with the inner wall in deposition portion 14, the isolated tube 2
Internal diameter is greater than the outer diameter of the suction nozzle of the suction pipe 3, so that suction nozzle is inserted into isolated tube 2.
The outer diameter of the suction nozzle of the suction pipe 3 is less than the internal diameter in the deposition portion 14, so that suction nozzle protrudes into 14 interior suction of deposition portion
Take sperm.
The convex ribs 21 have two, are separately positioned on the opposite sides of the lower port of isolated tube 2.
The width of the convex ribs 21 is gradually reduced from top to bottom.
The caliber of the isolated tube 2 is gradually reduced from top to bottom.
The separation of the utility model and the use step of extraction element are as follows:
1) isolated tube 2 is inserted into centrifuge tube 1, until the inner wall mistake of the convex ribs 21 of 2 bottom of isolated tube and deposition portion 14
It is full of cooperation, to support isolated tube 2；
2) as shown in figure 4, pouring into density gradient media 4；Since the lower port of isolated tube 2 is connected to tube bottom 13, density level bands
Degree medium 4 can flow between centrifuge tube 1 and isolated tube 2, keep unified liquid level；
3) as shown in figure 5, centrifuge tube 1 in (sky that isolated tube 2 outer wall and centrifuge tube 1 inner wall formed outer toward isolated tube 2
Between) injection sperm 5 to be processed, due to the setting of isolated tube 2, before centrifugally operated, sperm 5 will not be entered in isolated tube 2
Portion；
4) centrifugally operated of a period of time is carried out；After centrifugation, as shown in fig. 6, high-quality sperm can be situated between by density gradient
Matter 4 reaches tube bottom 13, and most of concentration accumulates in deposition portion 14, and sperm inferior then rests on upper layer, that is, isolated tube 2
Outside can not enter in isolated tube 2 via the bottom of isolated tube 2；
5) suction nozzle of suction pipe 3 is inserted into isolated tube 2, and the lower port for passing through isolated tube 2 reaches deposition portion 14, extracts high-quality
Sperm；In this process, the suction nozzle of suction pipe 3 only touches the density gradient media 4 in isolated tube 2, and density gradient herein
Without poor quality sperm in medium 4, deposition portion 14 and high-quality sperm contact are then directly reached, is avoided inferior in extraction process
The problem of sperm pollutes, improves the success rate and probability for extracting high-quality sperm.
Above description is only a specific implementation of the present invention, but the protection scope of the utility model is not limited to
In this, being familiar with the those skilled in the art should be understood that the utility model includes but is not limited to attached drawing and specific embodiment party above
Content described in formula.The modification of any function and structure principle without departing from the utility model is intended to be included in claims
Range in.
Claims (7)
1. separation and the extraction element of a kind of high-quality sperm, including centrifuge tube and suction pipe, centrifuge tube include the tube body of upper end opening
With cover the pipe lid at body open, it is characterised in that: the tube body includes the pipe shaft of straight wall and the tube bottom of back taper, tube bottom
Bottom rides out to form deposition portion downwards, and the deposition portion is in U-shape；The outer diameter of the suction nozzle of the suction pipe is less than the interior of the deposition portion
Diameter draws sperm so that suction nozzle protrudes into deposition portion.
2. separation and the extraction element of a kind of high-quality sperm according to claim 1, it is characterised in that: the deposition portion with
The pipe shaft coaxial arrangement.
3. separation and the extraction element of a kind of high-quality sperm according to claim 1, it is characterised in that: the deposition portion
Length is 3mm~12mm；The internal diameter in the deposition portion is 2mm~6mm.
4. separation and the extraction element of a kind of high-quality sperm according to any one of claim 1 to 3, it is characterised in that:
The separation and the isolated tube that extraction element includes hollow and upper and lower ends are open, isolated tube are located in centrifuge tube, isolated tube
Lower end extend to tube bottom and lower port is connected to tube bottom, the lower port of the isolated tube is equipped with the convex ribs extended downwardly, described convex
Muscle extend into deposition portion and is interference fitted with the inner wall in deposition portion, and the internal diameter of the isolated tube is greater than the suction nozzle of the suction pipe
Outer diameter, so that suction nozzle is inserted into isolated tube.
5. separation and the extraction element of a kind of high-quality sperm according to claim 4, it is characterised in that: the convex ribs have two
Item is separately positioned on the opposite sides of the lower port of isolated tube.
6. separation and the extraction element of a kind of high-quality sperm according to claim 4, it is characterised in that: the width of the convex ribs
Degree is gradually reduced from top to bottom.
7. separation and the extraction element of a kind of high-quality sperm according to claim 4, it is characterised in that: the isolated tube
Caliber is gradually reduced from top to bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821251002.8U CN208791633U (en) | 2018-08-03 | 2018-08-03 | A kind of separation of high-quality sperm and extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821251002.8U CN208791633U (en) | 2018-08-03 | 2018-08-03 | A kind of separation of high-quality sperm and extraction element |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208791633U true CN208791633U (en) | 2019-04-26 |
Family
ID=66204452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821251002.8U Active CN208791633U (en) | 2018-08-03 | 2018-08-03 | A kind of separation of high-quality sperm and extraction element |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208791633U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110533672A (en) * | 2019-08-22 | 2019-12-03 | 杭州德适生物科技有限公司 | A kind of chromosome sort method based on band identification |
-
2018
- 2018-08-03 CN CN201821251002.8U patent/CN208791633U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110533672A (en) * | 2019-08-22 | 2019-12-03 | 杭州德适生物科技有限公司 | A kind of chromosome sort method based on band identification |
| CN110533672B (en) * | 2019-08-22 | 2022-10-28 | 杭州德适生物科技有限公司 | Chromosome sorting method based on strip recognition |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208791633U (en) | A kind of separation of high-quality sperm and extraction element | |
| CN211585301U (en) | Draw jar and pilot scale and draw jar unit | |
| CN210151065U (en) | Leukemia cell separation and extraction device | |
| CN217438172U (en) | Cell sample draws subassembly | |
| CN210170942U (en) | Suction filtration device | |
| CN212404068U (en) | Fruit wine fermentation device capable of increasing aroma substances | |
| CN204910929U (en) | Laboratory is with constant temperature filter equipment | |
| CN220166204U (en) | Culture flask convenient to stem cell exosome is collected | |
| CN210221933U (en) | Food additive detects uses suction filter device | |
| CN209005266U (en) | A kind of high efficiency extraction tank | |
| CN203803147U (en) | Device for extracting effective components of dendrobium nobile | |
| CN111849683A (en) | Fruit wine fermentation device capable of increasing aroma substances | |
| CN219334259U (en) | Anti-overflow formula centrifuging tube | |
| CN219890817U (en) | Fodder detects sampling device | |
| CN210674322U (en) | Sucralose mother liquor workshop uses segmentation ware | |
| CN201141805Y (en) | Cell acquisition bottle | |
| CN106053150B (en) | Liquid acquisition device | |
| CN216237009U (en) | Cell supernatant's draw-out device | |
| CN220618879U (en) | Barrel-shaped sperm optimizing device | |
| CN104840223A (en) | Saliva sample collection container | |
| CN209202038U (en) | A kind of tealeaves soup extractor | |
| CN216798797U (en) | Pressurized column chromatography device | |
| CN215308774U (en) | Automatic continuous fat collecting system | |
| CN215195463U (en) | Liquid medicine concentrator | |
| CN218793962U (en) | A enrichment facility for chemical detection reagent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |