CN216237009U - Cell supernatant's draw-out device - Google Patents

Abstract

The utility model relates to the technical field of biological medicines, and discloses a cell supernatant extraction device, which has the technical scheme that the device comprises an extractor, wherein the extractor comprises a guide rod which is arranged in a hollow manner, one end of the guide rod is a connecting end, the connecting end can be connected with a power part for generating suction force for extracting supernatant, and the other end of the guide rod is provided with a suction head.

Description

Cell supernatant's draw-out device

Technical Field

The utility model relates to the technical field of biological medicines, in particular to a cell supernatant extracting device.

Background

When stem cells are cultured, the stem cells secrete components containing more than 500 proteins, the secretion is a supernatant of the stem cells, the components contain growth factors rich in information transfer substances, the growth factors are key for activating the cells, play an important role in restoring damaged tissues and cell functions in vivo, and particularly have an effect on delaying cell aging. The prior art method for extracting the supernatant of the stem cells generally uses a needle tube to extract a membrane for separating the supernatant from the cells, but the extraction method is complicated.

SUMMERY OF THE UTILITY MODEL

In view of the shortcomings of the prior art, the present invention provides a cell supernatant extraction device, which overcomes the above-mentioned shortcomings of the prior art.

In order to achieve the purpose, the utility model provides the following technical scheme: the cell supernatant extraction device comprises an extractor, wherein the extractor comprises a guide rod which is arranged in a hollow mode, one end of the guide rod is a connecting end, the connecting end can be connected with a power part which generates supernatant extraction suction, a suction head is installed at the other end of the guide rod, an extraction surface is formed on the surface of the suction head, a plurality of small holes used for separating supernatant from cells are formed on the surface of the extraction surface, and the diameter of each small hole is 0.1-0.2 micrometer.

As a further improvement of the utility model, the suction head is arranged in a hollow way, and the diameter of the suction head is larger than that of the guide rod.

As a further improvement of the utility model, one side surface of the sucker, which is far away from the guide rod, is convexly arranged outwards, so that the circular arc-shaped suction surface is formed, and the diameter of the suction surface is 10cm-20 cm.

As a further improvement of the utility model, the suction head is arranged in a column shape, and the diameter of the suction head is 3cm-8 cm.

As a further improvement of the utility model, the suction head and the guide rod are integrally formed or detachably connected.

As a further improvement of the utility model, the power part comprises a collecting bottle, a first conduit and a second conduit, the first conduit and the second conduit are both communicated with the interior of the collecting bottle, one end of the first conduit far away from the collecting bottle is connectable with the connecting end, and one end of the second conduit far away from the collecting bottle is connected with an air pump.

As a further improvement of the utility model, one side of the collecting bottle is provided with an insertion end communicated with the inside of the collecting bottle, the insertion end is in a hollow tubular shape, and one end of the second conduit can be sleeved on the insertion end.

As a further improvement of the utility model, the top opening of the collecting bottle is arranged to form a bottle mouth, a bottle stopper is arranged at the bottle mouth, and one end of the second conduit is arranged on the bottle stopper and communicated with the inside of the collecting bottle.

As a further improvement of the utility model, the connecting end is a pagoda head.

The utility model has the beneficial effects that: the suction head designed in the way can be provided with the suction surface with a larger area, so that the quick supernatant extraction is finished, and the suction amount is several times of that of the traditional needle tube type suction.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic perspective view of the present invention;

fig. 3 is a perspective view of the mechanism of the extractor of fig. 1 of the present invention.

Reference numerals: 11. a suction head; 12. a guide bar; 13. a connecting end; 2. a power member; 22. an insertion end; 23. a bottle stopper; 3. a first conduit; 4. a second conduit.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples. In which like parts are designated by like reference numerals. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "bottom" and "top," "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.

Referring to fig. 1-3, the cell supernatant extracting device of the present embodiment includes an extractor, the extractor includes a hollow guide rod 12, one end of the guide rod 12 is a connecting end 13, the connecting end 13 can be connected to a power component 2 for generating suction force for extracting supernatant, the other end of the guide rod 12 is provided with a suction head 11, the surface of the suction head 11 is provided with a suction surface, the suction surface is provided with a plurality of small holes for separating supernatant from cells, the diameter of the small holes is 0.1-0.2 μm, the small holes with such size can effectively separate supernatant from dry cells, the extracting device for extracting cell supernatant is designed in the present invention, compared with the existing extracting method using a needle, the extracting device for extracting cell supernatant is provided with a suction surface with a certain number of small holes for directly absorbing, without modifying the needle and installing a filter membrane, the efficiency of drawing up of individual supernatant is improved.

Specifically, the suction surface is made of metal materials or alloy materials, so that the suction surface has the advantages of pressure resistance, uniform stress, good high-temperature resistance, high intercommunicating pore content and the like.

Referring to fig. 1-3, the suction head 11 is hollow and has a diameter larger than that of the guide rod 12, and the suction head 11 with such a design can have a suction surface with a larger area, so as to achieve rapid supernatant extraction, and the suction amount is several times of that of the traditional needle tube type suction.

Referring to fig. 1, one side of the suction head 11, which is away from the guide rod 12, is arranged to protrude outwards so as to form an arc-shaped suction surface, the diameter of the suction surface is 10cm-20cm, and for some cases (such as cell dishes) adopting a basin body to load cell sap, the suction surface arranged in this way can obtain a larger contact surface with the cell sap so as to finish quick suction.

Referring to FIG. 2, the pipette tip 11 is a column-shaped configuration, the diameter of the pipette tip 11 is 3cm-8cm, and for some cases (such as beaker and graduated cylinder) using a cylinder for loading cellular fluids, the pipette tip 11 in this configuration can be extended into these containers smoothly, and the suction surface on the pipette tip 11 can be surrounded on the side and bottom of the pipette tip 11, so as to obtain a large suction area.

In one embodiment, the suction head 11 and the guide 12 are integrally formed or detachably connected.

Specifically, under the great condition of extraction supernatant velocity of flow, can adopt integrated into one piece's design for the self intensity of suction means is higher, when being difficult to damage, the problem of weeping still can not appear.

Specifically, under the condition that the flow rate of the extracted supernatant is low, the cell culture medium can be detachably connected (such as threads, splicing and the like, and the cell culture medium is matched with a leakage-proof device such as a sealing gasket and the like as much as possible), so that the cell culture medium can adapt to different containers for containing cell sap.

Referring to fig. 1-2, the power member 2 includes a collecting bottle, a first conduit 3 and a second conduit 4, both the first conduit 3 and the second conduit 4 are communicated with the inside of the collecting bottle, one end of the first conduit 3 far away from the collecting bottle is connectable with a connecting end 13, and one end of the second conduit 4 far away from the collecting bottle is connected with an air pump.

Specifically, the air pump can be replaced by a manual device with an air suction function, such as a syringe, so as to be used under the condition that the power cannot be supplied.

Referring to fig. 1-2, an insertion end 22 communicating with the inside of the collection bottle is arranged at one side of the collection bottle, the insertion end 22 is in a hollow tubular shape, one end of the second conduit 4 can be sleeved on the insertion end 22, the insertion end 22 is arranged at one side of the collection bottle, so that the collection of supernatant can be facilitated, the setting of the bottle mouth of the collection bottle cannot be influenced, and in the process of pouring out supernatant from the bottle mouth, the collection bottle only needs to be inclined away from the insertion end 22, and the supernatant cannot flow out from the insertion end 22.

Specifically, the top opening of the collecting bottle is arranged to form a bottle mouth, a bottle stopper 23 is arranged at the bottle mouth, and one end of the second conduit 4 is arranged on the bottle stopper 23 and communicated with the inside of the collecting bottle.

Furthermore, a hard tube penetrating through the upper end and the lower end of the bottle stopper 23 is arranged in the bottle stopper 23, and the second guide tube 4 is sleeved on the hard tube so as to be communicated with the inside of the collecting bottle, so that the design has the advantage of facilitating the installation between the second guide tube 4 and the bottle stopper 23.

Specifically, the insertion end 22 is disposed near the mouth of the collection bottle, so that the supernatant does not easily flow through the connection between the insertion end 22 and the collection bottle during the extraction process.

Specifically, the insertion end 22 is integrally formed with the collection bottle to ensure that no leakage occurs therebetween.

Referring to fig. 3, the connecting end 13 is a pagoda head which has good sealing performance and can effectively prevent the turning-over of the conditions such as liquid leakage and the like.

Specifically, the material of the collecting bottle includes but is not limited to glass, and it has transparent characteristic, can observe the height of liquid level in the collecting bottle in real time to it has certain rigidity, can not damage in the process of air pump extraction.

The working principle is as follows: when the supernatant of the stem cells needs to be collected, a container filled with stem cell liquid is firstly kept stand to ensure that the layered stem cell liquid is obtained;

then, two ends of the first conduit 3 are respectively sleeved and connected with the connecting end 13 and the inserting end 22, then the second conduit 4 is installed to one side, away from the collecting bottle, of the bottle plug 23, then an air pump is installed on the second conduit 4, after the preparation work is finished, the suction head 11 is immersed into the layered stem cell liquid, the air pump is opened, air in the collecting bottle is pumped outwards, so that negative pressure is generated in the collecting bottle, the stem cell supernatant sequentially passes through the suction head 11, the guide rod 12, the connecting end 13, the first conduit 3 and the inserting end 22 and enters the collecting bottle, large particles such as stem cells in the stem cell liquid cannot pass through small holes in the suction surface, and finally the extraction of the stem cell supernatant is finished.

The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above-mentioned embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the utility model may occur to those skilled in the art without departing from the principle of the utility model, and are considered to be within the scope of the utility model.

Claims (8)

1. An extraction device of cell supernatant is characterized in that: including the aspirator, the aspirator includes guide arm (12) that cavity set up, the one end of guide arm (12) is link (13), link (13) can be connected with power spare (2) that produce the suction of extraction supernatant, suction head (11) are installed to the other end of guide arm (12), suction head (11) surface is formed with the face of drawing, it is formed with a plurality of apertures that are used for supernatant and cell separation to draw the face surface, the diameter of aperture is 0.1-0.2 micron.

2. A cell supernatant extracting device according to claim 1, wherein: the sucker (11) is arranged in a hollow mode, and the diameter of the sucker is larger than that of the guide rod (12).

3. A cell supernatant extracting device according to claim 2, wherein: one side surface of the suction head (11) departing from the guide rod (12) is outwards protruded to form a circular arc-shaped suction surface, and the diameter of the suction surface is 10cm-20 cm.

4. A cell supernatant extracting device according to any one of claims 1 to 3, wherein: the suction head (11) and the guide rod (12) are integrally formed or detachably connected.

5. A cell supernatant extracting device according to claim 1, wherein: the power part (2) comprises a collecting bottle, a first conduit (3) and a second conduit (4), the first conduit (3) and the second conduit (4) are communicated with the inside of the collecting bottle, one end of the first conduit (3) far away from the collecting bottle can be connected with the connecting end (13), and one end of the second conduit (4) far away from the collecting bottle is connected with an air pump.

6. A cell supernatant extracting device according to claim 5, wherein: one side of the collecting bottle is provided with an inserting end (22) communicated with the inside of the collecting bottle, the inserting end (22) is in a hollow tubular shape, and one end of the second conduit (4) can be sleeved on the inserting end (22).

7. A cell supernatant extracting device according to claim 5, wherein: the collecting bottle is characterized in that an opening is formed in the top of the collecting bottle, a bottle stopper (23) is installed at the bottle opening, and one end of the second guide pipe (4) is installed on the bottle stopper (23) and communicated with the inside of the collecting bottle.

8. A cell supernatant extracting device according to any one of claims 5 to 7, wherein: the connecting end (13) is a pagoda head.

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